Analysis of neural stem/ progenitor cell proliferation

and fateJune 23-26, 2014

Salzburg, Austria

INMiNDTraining Course

Imaging of Neuroinflammation in Neurodegenerative Diseases

Registration

Fee• INMiND members: 200€• Academic institutions: 400€• Industry: 800€

DeadlineMay 23, 2014

Number of participants• 4 participants minimum

(below which the training course will be cancelled)• 6 participants maximum

For registration please contact:

INMiND Training OfficeCorinne CARREAUX Phone: +33 1 69 08 25 02 Email: inmind.training@cea.fr

Venue

Institute of Molecular Regenerative Medicine & Institute of Experimental Neuroregeneration from the Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University, Salzburg, Austria

© Paracelsus Universität/Wild+Team

Cover PhotoAdult neurogenesis in the dentate gyrus of the hippocampus (P. ROTHENEICHNER , L. AIGNER, S. COUILLARD-DESPRES)

In the hippocampal dentate gyrus of adult DCX promoter-DsRed reporter rats, newly generated neurons show an active DCX promoter (DsRed+, red), and associated DCX protein expression s detected by a goat polyclonal antibody (green) (nuclear counterstain DAPI in blue), published in L. Jiang et al. 2013, J Proteomics; 80C: 268-280.0

Training Site

The training site at the Paracelsus Medical University Salzburg offers several transgenic reporter mouse models for neuro-/gliogenesis and a core facility for histological analyses. This site developed expertise in optical in vivo and ex vivo imaging of neurogenesis and gliogenesis under physiological and neurodegenerative conditions and in cell culture systems for the analysis of neural progenitor biology.

© Salzburg .info

This training course is organized in co-operation with FWF Special Research Program (SFB) F44 „Cell Signaling in Chronic CNS Disorders”www.uibk.ac.at/pharmazie/pharmakologie/sfb-f44/

Training Objectives

Teaching of immunohistochemical techniques for the study of neural stem/progenitor cells (NPCs) proliferation and fate in vivo and in vitro, including the preparation and maintenance of neural progenitor cultures and the analysis of relevant parameters.

Duration4-days (28 hours) teaching packages in small groups

Methods25% lectures and 75% practical work

Lectures content and practical work• in vivo imaging of neurogenesis using

bioluminescence imaging• in vivo labeling and immunohistochemical

detection of proliferating cells using BrdU• survival and cell fate analyses using

immunohistochemistry• survival and cell fate analyses in transgenic mice

using tamoxifen-activable Cre recombinase and fluorescent reporter systems

• isolation and culture of NPCs

CreditsUp to 2 ECTS points can be given to the course participants.

Teachers

Lectures will be given by scientists from the Paracelsus Medical University (PMU) Salzburg, Austria.

Course Leaders• Ludwig AIGNER• Francisco J. RIVERA• Barbara KLEIN

Phone: + 43 662 44 2002 1281 Email: barbara.klein@pmu.ac.at

Scientific Advisors• Ludwig AIGNER• Sebastien COUILLARD-DESPRES• Francisco J. RIVERA• Sebastien ILLES• Julia MARSCHALLINGER• Barbara KLEIN

For further details on the course please visit www.uni-muenster.de/INMiND/training