Refining Margarine

Crypthecodinium cohnii:A natural DHA Factory

•20 % lipid content by weight which is 40 % DHA•Facultative heterotroph, which does not perform

photosynthesis •Growth rate of 0.0375 h-1 on glucose substrate•Uses glucose as a carbon source •Uses dead yeast from ethanol bottoms as a nitrogen

source•Requires dissolved oxygen for optimal growth•Grows in brackish (saline) water.

Fermentation

Travis Dallas Eric Graves Joaquin Martinez Chris McNinch Ramune O. Meskyte Charu SainiMentors: Dan Rusinak, Jeffery Perl, John Micheli

•Lack of DHA is associated with poor or slower retinal

development and brain development•Helps infants gain weight faster•Recommended 300 mg/day for pregnant or lactating

women

•A lack of DHA is associated with Alzheimer’s disease•Inhibits the growth of human colon carcinoma cells•Increases the efficacy of chemotherapy

•Infant Health

•Adult Health

C22 H32 O2

INGREDIENTS: INTERESTERIFIED SOYBEAN OIL, WATER, ALGAE OIL, NATURAL SEA SALT, MONO/DIGLYCERIDES, NATURAL LECITHIN, SORBIC ACID, BETA-CAROTENE (VITAMIN A), ERGOCALCIFEROL (VITAMIN D), DIACETYL (FLAVORING)

DegummingBleaching

Removal FFADeodorization

CentrifugeCrude SBO

Citric Acid

GumWater

Citric Acid

Citric AcidAcid Activated Clay

Enzyme Acid ActivateClay

Interesterification

Hardfat SBO

Emulsification Pasteurization

Water

Sea Salt

Lecithin

Diacetyl

Mono/Diglycerides

B-Carotenes

Sorbic Acid

Ergocaliferols

Sodium methoxide

Citric Acid

Soft Water

Sodium methoxideCitric AcidSoft Water

ScrapedSurface HeatExchanger

Packaging

Refrigerant

To Wholesaler

DissolvedAir

FloatationFermentation Centrifuge

CO2

Lysing

Glucose

Ethanol StillBottom

Antifoam

Water

Algae

Glucanex

SteamCoolingWater

Sterile air

Vent

Water

Air CO2

Water

NaOH

Steam

Steam

Salt

CIPSystem

DryerAlgae Cake to

Wholesaler

Docosahexaenoic Acid

HO

O

Main Fermenter

Cooling Water

Sparged Steam

Vented Air13

Sterile Air

Sterile Glucanex Sterile

Anti-foam

7

8

11

Filter 1

Filter 2

P- 8

Filter 3

TIC

14

Compressor 1Air

PIC

PIC

FIC

QIC

QIC

9

P- 3

TIC

10QIC

Visc

Foam

QIC

P- 6

Turbidity

QIC

QIC

HX-1Fermentation Controls

35,000 gallon Fermentor

Height 50 feet

Diameter 12 feet

Total Project Capital Cost 37,328,232.77 USD

Operating Costs 18,962,032.85 USD/Year Raw Materials 6,031,280.00 USD/Year Utilities 3,532,157.83 USD/Year Maintenance 2,030,000.00 USD/Year Operating Labor Costs 2,828,000.00 USD/Year Operating Charges 707,000.00 USD/Year Plant Overhead 2,429,000.00 USD/Year G and A Costs 1,404,594.96 USD/Year

Total Product Sales 25,963,238.43 USD/Year

Payback Period 5.7 Years

Net Present Value 84,727,200.17 USD

Internal Rate or Return 25.5%

-$50

$0

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0 5 10 15

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Cum

ula

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Cas

hflo

w

Cumulative Cash FlowBasis: 1% of Market

Year of Plant Life

-$100

-$50

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$50

$100

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$300

$350

0 5 10 15

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ns

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Cumulative Cash FlowBasis: 2% in 6 Years

Year of Plant Life

-$100

-$50

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$50

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$350

$400

0 5 10 15

Millio

ns

Cum

ula

tive

Cas

hflo

w

Cumulative Cash FlowBasis: 2% of Market

Year of Plant Life

Expand to 2% Market Share in 6 Years

Payback Period 7.13 Years

Net Present Value 158,394,244.01 USD

Internal Rate or Return 34.5%

2% Market Share from Start

Payback Period 5.00 Years

Net Present Value 198,627,853.09 USD

Internal Rate or Return 34.5%

Growth Phase

Maintenance

What is Sanitary Design?Preventing microbial growth and simply being clean

How do you go about this?Physical separations of the different plantsAll equipment composed of stainless steelMargarine Plant composed of polished stainless steelLinear and horseshoe shaped plant designClean in Place system (CIP) on the insideSpray cleaning foam on the outside

Who cares?You do, and so do we. We do not want you getting sick from our product.

Algae Oil

Alpha Algae Co.1928 SE Otis Rd.Cedar Rapids, IA 52401

Alpha Algae Co.

Alpha Algae Co.

Truck Engines

Just Spread It On!

Sooo Healthy!0g Trans Fatty Acids100% Vegan100% Daily Omega-3 Fatty Acids per Serving

Eat Premium Golden rather than me for your essential fatty acids. Butter your biscuits!

We like it clean!

You should get DHA from algae just like me. It is tons healthier for both of us.

Algae Growth Cycle

0

5

10

15

20

25

30

0 20 40 60 80 100

Time (Hours)

Ce

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sit

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g/L

)