Reduced background pollination?Ulfstand Wennström

Curt AlmqvistBengt Andersson

Why bother about BP?

10 %

Plus tree testing

• Large scale progeny-tests• Many locations• Different environments

25 %

40% of the pollen comes from outside the orchard

25 %20 %

20 %12.5 %

Our idea

15 dec 2009

15 dec 2009

15 dec 2009

15 dec 2009

28 apr 2010

07 maj 2010

05 maj 2010

Sex tunnels, three treatments + control

Treatments AntalControl 2Tunnel 2Tunnel + fan 2Tunnel + suplimental pollination (SP) 2

Control

Tunnel

Tunnel + fan

Tunnel + supplemental pollen (SP)

Clone Dedicated to: Clone Dedicated to:AC1006 Run Peng Wei Y3012 Jan-Erik NilssonAC1075 Johan Kroon Y3014 Tore EricssonAC2047 Katarina Lindgren Y4016 Finnvid PrescherAC2064 Seppo Ruotsalainen Y4103 Urban ErikssonAC3015 Torbjörn Lestander Y4507 Gösta ErikssonAC3033 Takeshi Torimaru Y4508 Nebi BilirAC3040 Curt Almqvist Z2081 Ola RosvallAC3056 Zheng Yongqi Z3007 Anders FriesAC3065 Adalsteinn Sigurgeirsson Z3009 Xiao-Ru WangAC4221 Shen Xi-Huan Z3029 Yoshinari MorugushiX4203 Yousry El-Kassaby Z4003 Lennart AckzellY2004 Eleonor White Z4019 Mohan VargheseY2005 Kyu-Suk Kang Z4022 Darius DanuseviciusY3001 Bengt Andersson Z4032 Ulfstand Wennström

Clone Dedicated to: Clone Dedicated to:AC1006 Run Peng Wei Y3012 Jan-Erik NilssonAC1075 Johan Kroon Y3014 Tore EricssonAC2047 Katarina Lindgren Y4016 Finnvid PrescherAC2064 Seppo Ruotsalainen Y4103 Urban ErikssonAC3015 Torbjörn Lestander Y4507 Gösta ErikssonAC3033 Takeshi Torimaru Y4508 Nebi BilirAC3040 Curt Almqvist Z2081 Ola RosvallAC3056 Zheng Yongqi Z3007 Anders FriesAC3065 Adalsteinn Sigurgeirsson Z3009 Xiao-Ru WangAC4221 Shen Xi-Huan Z3029 Yoshinari MorugushiX4203 Yousry El-Kassaby Z4003 Lennart AckzellY2004 Eleonor White Z4019 Mohan VargheseY2005 Kyu-Suk Kang Z4022 Darius DanuseviciusY3001 Bengt Andersson Z4032 Ulfstand Wennström

Same clonal mix in all treatments

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Flower development in seedorchard Västerhus 2011

Pollen catch 400 m NW

Pollen catch mid orchard 1

Pollen catch mid orchard 2

Pollen catch 400 m SE

Pollen shed tunnels

Pollen shed control

Receptive ♀-flowers tunnels

Receptive ♀-flowers con-trol

Tunnels ♀-max

Control ♀-max

T-sum Järved (nearby)

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Temperature-sum (+5°C)

1-May 11-May 21-May 31-May 10-Jun 20-Jun 30-Jun0

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ControlTunnel

One week

Cones and seeds, effect of treatment

Treatment Cones/graft Seeds/graft Seeds/cone 1000-sw

Control 39 A 595 A 12,7 A 7,57 C

Tunnel 29 A 255 A 6,8 B 8,51 A

Tunnel + fan 51 A 501 A 8,6 B 8,26 AB

Tunnel + SP 54 A 685 A 10,7 A 8,05 BC

CloneCones/

treeFilled seeds/

tree (g)Filled seeds/

tree (no) SeedweightSeeds/ cone

AC1075 11 1,3 170 8,35 5,9AC3015 20 1,3 154 8,89 4,5AC3040 27 1,4 214 7,40 6,5AC3056 53 5,4 721 7,65 12,9AC3065 90 5,6 869 6,52 10,7Y3012 21 0,6 80 7,46 3,4Y3014 33 2,0 234 8,90 7,2Z2081 59 6,3 845 7,90 16,0Y4103 3 1,3 175 8,33 14,9Z4032 113 15,7 1628 9,56 14,8

Cones and seeds, effect of clone

Pollen (g/graft),effect of treatment

Treatment Pollen

Control 51,5 A

Tunnel 64,3 A

Tunnel + fan 64,6 A

Tunnel + SP 58,8 A

Data from Xiau-Ru Wang, Torimaru Takeshi and Jinlong Zhang at EMG Umeå University

Preliminary results (480 seeds, 4 clones) anlyzed with microsatellites (SSRs)

Do the tunnels work?

* 48% of the fathers originated from the SP

Treatment Background pollination

Other than tunnel clones

Control 23.8 34.3Tunnel 2.2 2.1Tunnel + fan 0 0

Tunnel + SP 4.5 0*

Reduced background pollination,does it work?

Yes!