Recombinant DNA Technology

Bacteria, Yeast, & Virus Plasmids

What is a Plasmid?

• Circular DNA found in bacteria, viruses, and yeast

Recombinant Plasmids

• Plasmids that have foreign DNA inserted into them in a process called Recombinant DNA Technology

Methods of Recombinant Technology

• Transformation– Plasmid and foreign DNA

are cleaved by restriction enzymes

– Uses DNA ligase to insert DNA into vector

– Vector inserted into host cell (usually E. coli)

Methods of Recombinant Technology

• Non-Bacterial Transformation– Similar to transformation

except E. coli/bacteria is not used as a host cell

– DNA is injected directly into the nucleus of the cell being transformed

OR– The host cells are

bombarded with high velocity microprojectiles such as gold or tungsten coated with DNA

Methods of Recombinant Technology

• Phage Introduction– Process of transfection

(equivalent to transformation) except a phage is used instead of bacteria

– Lambda or M13 phages are used to produce phage plaques which contain recombinants

Antibiotic ResistanceThe Evolutionary Arms Race

http://www.youtube.com/watch?v=9CuhqQzBACQ (Ep1)

http://www.youtube.com/watch?v=2L82V6VPJkQ (Ep2)

http://www.youtube.com/watch?v=D0_FTJnhzXA (Ep3)

http://www.youtube.com/watch?v=ofL52tWhacY (Ep4)

http://www.youtube.com/watch?v=Chmb2g7atZ4 (Ep5)

http://www.youtube.com/watch?v=J-VOvideRZA (Ep6)