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Recombinant DNA technology. DNA isolated from two sources and cut by the same enzyme. Plasmid (vector). Gene of interest. Some uses of genes:. Some uses of proteins:. Recombinant DNA. Gene for pest resistance. Protein for dissolving clots. Gene for toxic-cleanup bacteria. - PowerPoint PPT Presentation
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Recombinant DNA technologyDNA isolated from
two sources and cut by the same enzyme
Gene of interest
RecombinantDNA
Plasmid(vector)
Transgenic organisms
Useful products
Gene for pestresistance
Gene fortoxic-cleanup
bacteria
Some uses of proteins:
Protein for“stone-washing”
jeans
Protein fordissolving
clots
Some uses of genes:
Restriction Endonucleases
Recombinant DNA technology
First DNA Cloning ExperimentCohen et al., 1973“Recombinant DNA”
Recombinant DNA technology
Cloning Vectors: pBR322 plasmid
Recombinant DNA technology
Genetically Modified (GM) FoodsIn the United States today, roughly one-half of the corn crop and over three-quarters of the soybean and cotton crops are genetically modified.
●Corn has been genetically modified to resist insect
infestation.
Golden RiceGenetically Modified (GM) Foods
Palmiter et al. (1982) Dramatic growth of mice that develop from eggs microinjected with metallothionine-growth hormone fusion genes. Nature 300: 611-615
“Supermice”
Recombinant DNA technology
Weeding Out Land Mines
First Glowing Pet
Recombinant DNA technology
Molecular Techniques•Nucleic Acid Extraction
Buffer Components:
SDS (sodium dodecyl sulfate)NaClSodium citrateEDTA
•Polymerase Chain Reaction
