Craigiebuckler, Aberdeen, AB15 8QH, UK

RECIPE Baupte Progress meeting

08-10/11/2004

UK progress

Rebekka Artz

Stephen Chapman

Colin Campbell

I. WP01 UK Field site data

• Middlemuir Moss, between Strichen and New Pitsligo, NE Scotland

Dark CO2 production

> 50 years

-1000

0

1000

2000

3000

4000

5000

6000

7000

0 100 200 300 400

time (d)

CO

2 (

mg

m-2 d

-1)

D1 D2 D3

Sphagnum 5 - 10 years

-1000

0

1000

2000

3000

4000

5000

6000

7000

0 100 200 300 400

time (d)

CO

2 (

mg

m-2 d

-1)

B1 B2 B3

1st data point: 30/09/03

Bare surface

-1000

0

1000

2000

3000

4000

5000

6000

7000

0 100 200 300 400

time (d)

CO

2 (

mg

m-2 d

-1)

A1 A2 A3

Eriophorum 5 - 10 years

-1000

0

1000

2000

3000

4000

5000

6000

7000

0 100 200 300 400

time (d)

CO

2 (

mg

m-2 d

-1)

C1 C2 C3

CO2 fluxes during photosynthesis

Eriophorum 5 - 10 years

-6000

-5000

-4000

-3000

-2000

-1000

0

1000

0 100 200 300 400

time (d)

CO

2 (

mg

m-2

d-1

)

C1 C2 C3

Bare surface

-6000

-5000

-4000

-3000

-2000

-1000

0

1000

0 100 200 300 400

time (d)

CO

2 (

mg

m-2 d

-1)

A1 A2 A3 Sphagnum 5-10 years

-6000

-5000

-4000

-3000

-2000

-1000

0

1000

0 100 200 300 400

time (d)

CO

2 (

mg

m-2

d-1)

B1 B2 B3

> 50 years

-6000

-5000

-4000

-3000

-2000

-1000

0

1000

0 100 200 300 400

time (d)

CO

2 (

mg

m-2

d-1

)

D1 D2 D3

CO2 Net Ecosystem ExchangeBare surface

-5000

-4000

-3000

-2000

-1000

0

1000

2000

3000

0 100 200 300 400time (d)

CO

2 (

mg

m-2 d

-1)

A1 A2 A3 Sphagnum 5 - 10 years

-5000

-4000

-3000

-2000

-1000

0

1000

2000

3000

0 100 200 300 400time (d)

CO

2 (

mg

m-2 d

-1)

B1 B2 B3

Eriophorum 5 - 10 years

-5000

-4000

-3000

-2000

-1000

0

1000

2000

3000

0 100 200 300 400time (d)

CO

2 (

mg

m-2 d

-1)

C1 C2 C3 > 50 years

-5000

-4000

-3000

-2000

-1000

0

1000

2000

3000

0 100 200 300 400time (d)

CO

2 (

mg

m-2 d

-1)

D1 D2 D3

CH4 ProductionBare surface

-5

0

5

10

15

20

0 100 200 300 400

time (d)

CH

4 (

mg

m-2

d-1

)A1 A2 A3

-100

0

100

200

300

0 100 200 300 400

A1 A2 A3

Sphagnum 5 - 10 years

-5

0

5

10

15

20

0 100 200 300 400

time (d)

CH

4 (

mg

m-2

d-1

)

B1 B2 B3

Eriophorum 5 - 10 years

-5

0

5

10

15

20

0 100 200 300 400

time (d)

CH

4 (

mg

m-2

d-1

)

C1 C2 C3 > 50 years

-5

0

5

10

15

20

0 100 200 300 400

time (d)

CH

4 (

mg

m-2

d-1

)

D1 D2 D3

exetainers

exetainers

exetainers

exetainers

Water tableBare surface

-100

-80

-60

-40

-20

0

20

0 30 60 90 120 150 180 210 240 270 300 330 360 390 420

time (d)

wat

er t

able

(cm

)well 1 well 2 well 3 Sphagnum 5-10 years

-100

-80

-60

-40

-20

0

20

0 30 60 90 120 150 180 210 240 270 300 330 360 390 420

time (d)

wat

er t

able

(cm

)

well 1 well 2 well 3

Eriophorum 5-10 years

-100

-80

-60

-40

-20

0

20

0 30 60 90 120 150 180 210 240 270 300 330 360 390 420

time (d)

wat

er t

able

(cm

)

well 1 well 2 well 3 > 50 years

-100

-80

-60

-40

-20

0

20

0 30 60 90 120 150 180 210 240 270 300 330 360 390 420

time (d)

wat

er t

able

(cm

)

well 1 well 2 well 3

11/08/2003

13/11/2003

6/05/2004

Other parametersair temperature

-5

05

10

1520

25

0 50 100 150 200 250 300 350 400

time (d)

A1 A2 A3 B1 B2 B3 C1 C2 C3 D1 D2 D3

soil surface temperature

-5

05

10

1520

25

0 50 100 150 200 250 300 350 400

time (d)

A1 A2 A3 B1 B2 B3 C1 C2 C3 D1 D2 D3

soil temperature @ -1 cm

-5

0

5

10

15

20

0 50 100 150 200 250 300 350 400

time (d)

A1 A2 A3 B1 B2 B3 C1 C2 C3 D1 D2 D3

soil temperature @ -5 cm

-5

0

5

10

15

20

0 50 100 150 200 250 300 350 400

time (d)

A1 A2 A3 B1 B2 B3 C1 C2 C3 D1 D2 D3

1st data point: 18/12/03

PAR

0

500

1000

1500

2000

0 50 100 150 200 250 300 350 400

time (d)

um

ol/

m2/

s

A1 A2 A3 B1 B2 B3 C1 C2 C3 D1 D2 D3

1st data point: 12/02/04

Calluna vulgaris

Erica tetralix

Molinia caerulea

Agrostis canina

Deschampsia f lexuosa

Eriophorum vaginatum

Eriophorum angustifolium

Juncus bulbosis

Sphagnum palustre

Sphagnum capillifolium

Sphagnum auriculatum

Sphagnum cuspidatum

Aulacomium palustre

Hypnum jutlandicum

Polytrichum commune

Campylopus introflexus

liverw orts

Narthecium ossifragum

Potentilla erecta

Drosera rotundifolia

Dryopteris (dilata?)

Pleurozium schreberi

bare

litter

A

B

C

D

Vegetation cover: Collars (0.07 m2)

Calluna vulgaris

Erica tetralix

Molinia caerulea

Agrostis canina

Deschampsia f lexuosa

Eriophorum vaginatum

Eriophorum angustifolium

Juncus bulbosis

Sphagnum palustre

Sphagnum capillifolium

Sphagnum auriculatum

Sphagnum cuspidatum

Aulacomium palustre

Hypnum jutlandicum

Polytrichum commune

Campylopus introflexus

liverw orts

Narthecium ossifragum

Potentilla erecta

Drosera rotundifolia

Dryopteris (dilata?)

Pleurozium schreberi

bare

litter

A

B

C

D

Vegetation cover: Grids (1 m2)

II. WP2 Progress

-50

-40

-30

-20

-10

0

10

20

0 20 40 60 80 100

Time (d)

wat

er t

able

bel

ow

su

rfac

e (c

m)

A low B low C low

-50

-40

-30

-20

-10

0

10

20

0 20 40 60 80 100

Time (d)

wat

er t

able

bel

ow

su

rfac

e (c

m)

A median B median C median

-50

-40

-30

-20

-10

0

10

20

0 20 40 60 80 100

Time (d)

wat

er t

able

bel

ow

su

rfac

e (c

m)

A high B high C high

dark CO2 fluxes - HIGH WT

-1000

-800

-600

-400

-200

0

200

400

600

800

0 25 57 90 121

time (d)

BARESPHAGNUM E. VAGINATUM E. ANGUSTIFOLIUM

dark CO2 fluxes - LOW WT

-1000

-500

0

500

1000

0 25 57 90 121

time (d)

BARESPHAGNUM E. VAGINATUM E. ANGUSTIFOLIUM

dark CO2 fluxes - MEDIAN WT

-800

-600

-400

-200

0

200

400

600

0 25 57 90 121

time (d)

BARESPHAGNUM E. VAGINATUM E. ANGUSTIFOLIUM

Dark CO2 production

CO2 fluxes during photosynthesis

light CO2 fluxes - LOW WT

-2000

-1500

-1000

-500

0

500

1000

0 25 57 90 121

time (d)

BARESPHAGNUM E. VAGINATUM E. ANGUSTIFOLIUM

light CO2 fluxes - HIGH WT

-1800-1600-1400-1200-1000-800-600-400-200

0200400

0 25 57 90 121

time (d)

BARESPHAGNUM E. VAGINATUM E. ANGUSTIFOLIUM

light CO2 fluxes - MEDIAN WT

-2000

-1500

-1000

-500

0

500

1000

0 25 57 90 121

time (d)

BARESPHAGNUM E. VAGINATUM E. ANGUSTIFOLIUM

CH4 Productiondark CH4 fluxes - LOW WT

-1

1

3

5

7

9

11

0 25 57 90 121time (d)

mg

CH

4 m

-2 d

-1

BARESPHAGNUM E. VAGINATUM E. ANGUSTIFOLIUM

dark CH4 fluxes - HIGH WT

-1

1

3

5

7

9

11

0 25 57 90 121time (d)

mg

CH

4 m

-2 d

-1

BARESPHAGNUM E. VAGINATUM E. ANGUSTIFOLIUM

dark CH4 fluxes - MEDIAN WT

-1

1

3

5

7

9

11

0 25 57 90 121time (d)

mg

CH

4 m

-2 d

-1

BARESPHAGNUM E. VAGINATUM E. ANGUSTIFOLIUM * caused by

single outlier (195 mg m-2 d-1); other two <10.

Further work:

• 1st harvest expected to take place either end of november or mid march (pending decision)

• Clare Trinder will use the field site for litter bag experiments

III. Fungal community structure analysisby DGGE of ribosomal 18S and/or ITS sequences

SC

M

FR

FI

FBCH

Canonical variate 1

0

6

2

-2

42

4

0

-4

8

-2-4-6

Ca

no

nica

l varia

te 2

E

A

D

C

B

Canonical variate 1

0-2 4

-4

2

2

-4

-6

0

-2

4

-6

Ca

no

nica

l varia

te 2

D

A

C

B

Canonical variate 1

3

10

-1

1

3-1-2

2

-2

0

2

Ca

no

nica

l varia

te 2

D

A

C

B

Canonical variate 1

2

42

-1

8

1

0-2

0

-2

6

Ca

no

nica

l varia

te 2

D

A

CB

Canonical variate 1

2

-1

0-1

1

3

-2

-2 3-3

4

0

2

1

Ca

no

nica

l varia

te 2

E

A

D

C

B

Canonical variate 1

0-2 4

-4

2

2

-4

-6

0

-2

4

-6

Ca

no

nica

l varia

te 2

D

A

C

B

Canonical variate 1

3

10

-1

1

3-1-2

2

-2

0

2

Ca

no

nica

l varia

te 2

D

A

C

B

Canonical variate 1

2

42

-1

8

1

0-2

0

-2

6

Ca

no

nica

l varia

te 2

D

A

CB

Canonical variate 1

2

-1

0-1

1

3

-2

-2 3-3

4

0

2

1

Ca

no

nica

l varia

te 2

A B

C D

CVA of fungal ITS DGGE patterns per country, grouped by site.

Figures show Finland (A); Scotland (B); France Russey (C) and Switzerland (D).

III. Fungal community structure analysis

• Clone library from DNA-derived ITS fragments has been prepared

• now cloned fragments are typed for RFLP type (in order to minimise no. of clones to be sequenced)

IV. Community level physiological profiling

• Method optimised for use with peat; 48 h incubation period; 14C-labeled C sources used:

N-Acetyl-Glucosamine

Glucose Benzoic acid

Galactose Phenylethylamine

Arabinose Glycine

Xylose Lysine

Sucrose Arginine

D-Mannitol Aspartic acid

Glucosamine Glutamic acid

SC

CH

FRFI

FB

Canonical variate 1

4

-2

-2 2

4

0

2

0

-4

Ca

no

nical varia

te 2

Other data are needed to perform CCA.

E

D

C

B

A

Canonical variate 1

-2

3

1

-1

21

4

0

0

-1

2

-2-3-4

Ca

non

ical varia

te 2

D

A

C

B

Canonical variate 1

210

-1

1

3

3

-1 4-2

2

-2

0

Ca

non

ical va

riate

2

D

A

C

B

Canonical variate 1

-3

-1

1

0-2

3

4

-2

0

-4-6

2

4

2

-4

Can

on

ical varia

te 2

D

ACB

Canonical variate 1

2

-2

0-2

0

2

4

-3

-4-6

3

-1

1

Ca

no

nical va

riate

2

A B

C D

CVA of CLPP patterns per country, grouped by site.

Figures show Finland (A); Scotland (B); France Russey (C) and Switzerland (D).

V. FTIR (preliminary analysis)

SC

CHFRFI

FB

Canonical variate 1

820

-4

-2

0

-4

4

-6

8

4

-2

6

2

10

6

Can

onica

l variate

2

E

D

C

B

A

Canonical variate 1

3

1

-1

-3

321

2

0

-2

-1-2

-4

0

-3-4-5

Can

onica

l variate 2

D

A

C

B

Canonical variate 1

4

-3

-1

0-2

1

3

-4

-2

-4-6

0

2

2

Ca

non

ical variate

2

D

A

C

B

Canonical variate 1

-2

2

-4 0-6

-4

0

-2 2

Can

onical variate

2

DA

C

B

Canonical variate 1

-2

0

0-2

2

4

4

-3

-1

-4 6-6

1

3

2

Can

onical varia

te 2

A B

C D

CVA of FTIR patterns per country, grouped by site.

Figures show Finland (A); Scotland (B); France Russey (C) and Switzerland (D).