Receiving Sample in Histo Lecture New

7/30/2019 Receiving Sample in Histo Lecture New

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INTRODUCTION

What is HistopathologyHistopathology(compound of two Greek word's: Histo",pathology ")Histo mean tissue and Pathology which mean thestudy of the essential nature of a disease and the abnormalityof structure and function .histopathology refers to the

examination of a biopsy or surgical specimen by a pathologist,.

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Check following things and match them on slip,

container and in history

Patient nameAgeGenderPatient mobile or Land lined #Patients Doctor nameSpecimen type

Clinical detailComplete history of patient

IMPORTANT POINTS

FOR SAMPLE COLLECTION AND TRANSPORTION

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Complete historyComplete history mean tissue biopsy site should be mentioned

what type of tissue is sending for histopathology and what is theclinical diagnosis of patient.

Must match the tissue type mentioned on container and on

form (both should be same)If on form it is written that tissue for histopathology is not a

complete historyWhat is sent for histopathology and why (specific diagnosis)In clinical history must make sure that cytology sample is not

for histopathology (as body fluid)Similarly make sure that biopsy sample is not for cytology

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Surgical sample should be

in formalinIf you received biopsy sample must check it is

received in formalin or without formalin.If received without formalin then add formalin

Always check the level of formalinTissue should be properly dip in formalinHowever there should not be over loaded

formalin that may cause spill due to leakage

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History check for biopsy

containerIf in history two containers are mentioned make sure that you

received two containers.Make sure both containers are for histopathology

Check history and request if both are for histopathology or oneis for cytology or microbiologyIf both containers are for histopathology make sure that both

should be in formalin.Above policy is applicable if multiple containers are received .Must check coding of each containers

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Proper containerSample should be in proper container.Preferred containers are open mouth plastic container withformalin up to appropriate level.

If needed change container such conditions could be when tissue islarge size but container is small ,sharp bin ,plastic bags or glassbottle should be replaced .While changing container be careful for placing all tags and tagshaving sample information on bottle put them on new transferredcontainer.All detailed information should match with those written oncontainer.If there is no tissue or empty container it could be return andrefund as well.proper handling of tiny tissue

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Requirement of SamplePBCR --- pathological block cutting report(block and previous report required)

SC ------------Slide for consultationRequest slip and previous report required it is basically FNAC related.

SL-G ------- slide given to patient (slide and blocks are return to patient)

BONE----------- x-ray is required.

Autopsy ----------- Consent form is required

FROZEN SAMPLE ----------Fresh sample

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ER.PR -----block or tissue

Her-2new -------block or tissue

ANAG---------2ml blood or serum in jell tube

Flow cytometry ---------3-5cc blood /bone marrow in EDTAor Sodium heparin

PH-MUSCLE --------- Tissue should be placed in acontainer having gauze piece soaked with N/S to avoid

tissue from dryness PI- IMMUNO FLOURESCENCE ----- Tissue should be placed in

a container having gauze piece soaked with N/S to avoidtissue from dryness or Zeus fixative--

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ALWAYS CHARGE ACCORDING TO LIST IFNEEDED ASK FROM DEPARTMENT BUT CHARGECORRECTLY

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PREPRATION OF

BUFFERED FORMALIN

37% to 40% formalin 100 mlD/W 900ML

Sodium phosphate mono basic 4.0 gmSodium phosphate dibasic 6.5gm

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Retention and storage

policy (surgical)Request slips --- 6 monthSpecimen ---5 weeksSlides ---20 yearsBlocks -- Indefinite periodX-rays -----return with reportPBCR ----blocks return to patient

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CYTOLOGY SAMPLE AND STORAGE POLICYSPUTUM ,URINE, PAP SMEAR ,FNA,FNACP,BODY

FLUIDNO ADDED ANY PRESERVATIVE

REQ FORMS 6 MONTH

SAMPLE-- TILL REPORTING

SLIDES----10 YEARS

BLOCKS== Indefinite period

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SPECIAL PATHOLOGY

IF --------slides stored up to reporting

Enzyme slides ---------1 yearBlock -------------Indefinite period

ANA ---------- positive samples stored up to 1 month

Negative sample ------------- stored up to 1 week

Flowcytometry sample ----------Till Reporting

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PROTOCOL OF REPORTING DATE

SMALL BX---4 WORKING DATE

MEDIUM BX----------5 WORKING DATELARGE BX--------------6 WORKING DATE

BONE BX------------------10WORKING DATE

EYE---------------10WORKING DATE

PBCR---------------7 WORKING DATE

SL-G---------------3 WORKING DATE

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Thank you

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Receiving Sample in Histo Lecture New