Innovation with IntegrityMALDI-TOF

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rapifleX MALDI Tissuetyper™ – Customer Feedback

For Mass Spectrometric Imaging

rapifleX™MALDI Tissuetyper™

MALDI Tissuetyper™Speed | Robustness | Definition

The rapifleX instrument re-defines the key performance measures for MALDI Imaging:

• Up to 50 true pixels / second for faster and better images

• smartbeam™ 3D laser for better pixel-to-pixel reproducibility

• Pixel size < 20µm for highest spatial resolution to retrieve biological information

• Novel ion source design for increased robustness

Lipids in rat testis, pixel size 10 µm, 395000 pixels, 200 shots per pixel, measurement time 165 minutes.

smartbeam™ 3D laser

During the image acquisition the sample stage moves continually to achieve high pixel rates. The newly developed smart-beam™ 3D laser fires at a repetition rate of up to 10 kHz.

The new smartbeam™ 3D laser features a a laser beam diameter of 5µm. As illu-strated below, the laser can move inde-pendently from the sample stage to scan the full area of each pixel. This produces truly square pixels which utilizes all of the available sample area for maximum sen-sitivity and pixel-to-pixel reproducibility.

Laser and Ion Source Designed for MALDI Tissue Typing

Ablated matrix showing square pixels.

Dual rotating mirrors raster the laser beam in an x-y axis which is independent of the x-y motion of the target. The two independent movements combine to create the ideal sampling environment for Imaging: the true, well-defined pixels, as seen above, acquired at maximum acquisition rate.

Novel Ion Source Design for Robust Day-to-Day Operation

• Extends uptime between cleaning by orders of magnitude.• Easy access to self-aligning ion lenses when cleaning is in fact needed.

Proteins in sagittal rat brain section measured at 30 µm pixel size, ~150000 pixel.

• The whole lens pack can be rinsed with Ethanol.

flexImaging

allows to set up and analyze individual datasets.

• Wizard driven user-friendly set up of new experiments• True 64-bit application allows efficient handling of megapixel datasets• Direct access to single mass spectra• Batch acquisition• Co-register and super-impose virtual microscopic slides at full resolution for detailed histological annotation

SCiLS Lab

statistical analysis software for mining MALDI Tissuetyper data. It allows both the analysis of individual datasets and the comprehensive analysis of clinical and pre-clinical studies.

• User interface to handle multiple datasets• Univariate analysis: Mean intensities, standard deviations, uncovering discriminative m/z-markers (ROC analysis), hypothesis tests and discovery of co-localized m/z values• Unsupervised multivariate analysis: Spatial segmentation of large datasets, component analysis for extraction of underlying trends• Supervised tissue typing: Calculation of classifiers based on training data and classification of new observations / samples

SCiLS Lab 3D

With the unparalleled speed of the rapifleX, 3D MALDI Tissuetyper studies are within reach. The SCiLS Lab 3D option allows the reconstruction, visualization, and statistical analysis of 3D Imaging datasets.

Leading Software for MALDI Tissuetyper

MALDI Imaging data overlaid with virtual microscopic slide in flexImaging

3D volume reconstruction and spatial segmentation of a kidney sample in SCiLS Lab.

Analysis of a clinical dataset with 21 independent patient samples in SCiLS Lab.

Tissue Typing – A Clinical Discovery Workflow

Tissue samples are measured in the rapifleX MALDI Tissuetyper.

Statistical analysis of MALDI Tissuetyper data in SCiLS Lab.

MALDI Imaging dataset overlaid with micro-scopic image allows detailed annotation.

Tissue is scanned in a microscopic slide scanner.

Tissue is histologically stained after the MALDI measurement.

Overlay of MALDI Tissuetyper image with microscopic image acquired from the same tissue section provides a clear link to

histology.

• MALDI time-of-flight system• New ion source for robust day-to-day operation• Novel smartbeam™ 3D laser• Fast acquisition of up to 50 true square pixel / second• Laser repetition rate 10 kHz• Pixel size <20µm

rapifleX MALDI Tissuetyper™ – Designed for MALDI Imaging

Integrated software solutions

• flexImaging dedicated molecular histology software controls data acquisition, data visualization, annotation and integration of virtual microscopic slides• SCiLS Lab is the dedicated software for statistical analysis. From individual imaging datasets to complete clinical biomarker studies, it features univariate and multivariate methods to mine the data and to find relevant information.

Proteins in gastric cancer, pixel size 30 μm, 99000 pixels, 500 laser shots per pixel, measure-ment time 82 minutes.

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“The ideal imaging MS system needs high data acquisition speeds, high spatial resolution capabilities, and high mass resolving power. With this new system, Bruker re-imagined the MALDI source and laser optics to deliver very impressive performance and information throughput. This is a revolutionary instrument which has the potential to significantly improve sample throughput and offer useful informa-tion in real time in clinical research.”

Dr. Pierre Chaurand, Associate Professor, Université de Montréal, Canada

“Translational clinically oriented research with imaging mass spectrometry is critically dependent on speed and robust operation. Current instrumentation is limiting its full adaptation. The rapifleX MALDI Tissuetyper allows us to finally overcome that limitation. It enables us to engage in high throughput clinical studies and establishes mass spectrometry imaging as a powerful and reliable information source for personalized medicine research.“

Dr. Ron M.A. Heeren, Director M4I, Maastricht University, The Netherlands

rapifleX MALDI Tissuetyper™ – Customer Feedback

“The analysis of tumor heterogeneity is one of the most important biomedical research challenges in oncology and personalized medi-cine. MALDI imaging is perfectly suited to get an unbiased molecular view on tumor heterogeneity. The speed and performance of the rapifleX MALDI Tissuetyper gives us for the first time the possibility to analyze even large tumor specimen over huge patient cohorts with sufficient spatial resolution to get a comprehensive understanding of tumor heterogeneity. “

Dr. Axel Walch, Institute of Pathology, Helmholtz-Centre Munich, Germany

Bruker Daltonik GmbH

Bremen · GermanyPhone +49 (0)421-2205-0

Bruker Scientific LLC

Billerica, MA · USA Phone +1 (978) 663-3660

ms.sales.bdal@bruker.com - www.bruker.com

For research use only. Not for use in diagnostic procedures.