J ALLERGY CLIN IMMUNOL

FEBRUARY 2014

AB196 Abstracts

MONDAY

677 Racial Differences In The Relationship Of Total and Food-Specific IgE To Atopic Dermatitis In Childhood

Dr. Gillian Bassirpour, MD1, Dr. Edward M. Zoratti, MD, FAAAAI2,

Ganesa Wegienka, PhD3, Ms. Suzanne Havstad, M.A.3, Alexandra

Sitarik, MS3, Dr. Haejin Kim, MD1, Dr. Dennis Ownby, MD, FAAAAI4,

Dr. Christine Cole Johnson, PhD, MPH, FAAAAI3; 1Division of Allergy

and Clinical Immunology, Henry Ford Hospital, Detroit, MI, 2Henry

Ford Health System, Detroit, MI, 3Department of Public Health Sciences,

Henry Ford Hospital, Detroit, MI, 4Department of Pediatrics Georgia Re-

gents University, Augusta, GA.

RATIONALE: Elevated IgE is a hallmark of atopic dermatitis (AD).

Although AD and elevated IgE are more prevalent in African American

(AA) compared to Caucasian (C) children, it is unknown if the relationship

between AD and IgE is similar between these groups.

METHODS: Data were analyzed from 371 AA and 138 C children in the

DetroitWHEALS birth cohort. ADwas defined as current or past physician

diagnosis as determined at a study-related encounter at 2-3 years of age.

Total and food-specific IgE (sIgE) levels for peanut, egg, and milk were

determined. Univariate and multiple logistic regression models, stratified

by race of child, were used to assess the association of AD with both total

IgE and food sensitization. Food sensitization was included in the model as

either the sum of the 3 food sIgEs or, separately, having at least one positive

sIgE (>_0.35 kU/L) to food.

RESULTS: Elevated total IgEwas independently associatedwith ADonly

among Caucasian children [adjusted odds ratio (aOR) 1.81, 95% CI 1.03,

3.15, p5 0.038]. In contrast, the sum of the sIgEs for the foods [aOR 1.46,

95% CI 1.15, 1.86, p 5 0.002] or the presence of >_ 1 positive food sIgE

[aOR 2.05, 95% CI 1.09, 3.87, p 5 0.027] was independently associated

with AD among AA children only.

CONCLUSIONS: The association of IgE to AD differs among AA and C

children. Food sIgE is independently linked to AD among AA children.

Such an association is not evident in C children after adjustment for total

IgE.

678 Major Culprit Allergen Sensitization Patterns According ToAge In Korean Atopic Dermatitis Patients

Hye Jung Park1, Jae-Hyun Lee1,2, Kyung-Yong Jeong2, Kyung-Hee

Park1,2, Yoon-Ju Kim2, Jung-Won Park1,2; 1Division of Allergy and Clin-

ical Immunology, Department of Internal Medicine, Yonsei University

College of Medicine, Seoul, South Korea, 2Institute of Allergy, Yonsei

University College of Medicine, Seoul, South Korea.

RATIONALE: Atopic dermatitis (AD) usually occur in childhood and

some may be remitted but others persist to adulthood. Culprit allergens

may be changed as the disease progress. In this study, we tried to

distinguish important culprit allergens according to age in AD patients.

METHODS: Total 110 AD patients’ sera were tested to detect allergen

specific immunoglobulin E (sIgE) for each 11 allergens - milk, egg white,

peanut, shrimp, wheat,Dermatophagoides farinae (DF),Candida albicans

(CA), Trichophyton rubrum (TR), Pityrosporum ovale(PO),

Staphylococcal enterotoxin B (SBE), recombinant Hom s 1. Mean age of

the enrolled patients was 12.2 years olds (range: 1;47).

RESULTS: Sensitization rates of allergens were varied from 17.3% (for

TR) to 52.7% (for DF). Milk and egg white sIgEs were more detected in

young aged patients and their sensitization rates decreased according to

age increment. Conversely, sensitization rates of shrimp, DF, SBE, CA,

TR, PO and SBE increased according to age increment. There were no

differences in peanut and wheat sensitization rates. Interestingly, rHom s 1

sIgE was detected more frequently in younger patients (36.5% in age 1-5

vs. 12.1% in age >_ 18).

CONCLUSIONS: There was distinct difference of sensitization pattern.

Milk and egg white are more important allergens in young aged patients.

On the contrary, microbial and house dust mite allergens were more

important in adult patients. Autoantigen can also be important cause at

early stage of AD.

679 Protein Microarray: IgE-Profiling Of Brazilians With AtopicDermatitis

Lucila Camargo Lopes de Oliveira1, Roberta Faria Camilo-Ara�ujo1, Isa-

bel Rugue Genov1, Dr. Renata R. Cocco2, Dr. Marcia Mallozi, MD3,

Prof. Nelson A. Rosario, MD, PhD, FAAAAI4, Prof. Dirceu Sole, MD,

PhD5; 1UNIFESP, 2Federal University of S~ao Paulo, 3Federal University

of S~ao Paulo, Brazil, 4Federal University of Parana, Curitiba, Brazil, 5Fed-

eral University of Sao Paulo, Sao Paulo, Brazil.

RATIONALE: to identify for the first time the IgE-sensitization profile

among Brazilian patients with different severity of atopic dermatitis (AD)

using protein microarray techniques. We hypothesized the greater the

severity the disease, the more common the sensitization to food allergens.

METHODS: 59AD patients classified according to the severity scoring of

AD index (SCORAD,SI) took part on this study. Peripheral blood samples

were collected after consent approval and their IgE-sensitizing profile was

determined using ImmunoCAP-ISACTM (Thermo Scientific). The study

was approved by the Brazilian ethical committee (#

12551413.2.0000.5505 on www.saude.gov.br/plataformabrasil, funded

by FAPESP no 2009/53303-3). Data were expressed as mean, median

and range. Kruskal-Wallis test was used to analyze differences among

groups since they had no normal distribution.

RESULTS: patients aged from 0.75 to 23.4 years (mean 5 8.25y) were

distributed as following: mild (A, n517, median age5 12.2y, mean SI 523.7), moderate (B, n513, median age 5 8.5y, mean SI 5 42.25), and

severe (C, n529, median age58.0y, mean SI5 54.64).Mean sensitization

(number of allergens per individual) to all allergens [A:8,6 (range 2-38),

B:14,7 (6-41), C:13,3 (1-39)] and just to food allergens [A:1,7 (0-18);

B:3,8 (0-21); 3,4 (0-19)] were not significant different between groups

(Kruskal-Wallis test, p50.0641).

CONCLUSIONS: food sensitization was not related to intensity of atopic

dermatitis on this Brazilian studied group.

680 Relationship Between Dietary Food and Nutrient Intakes andBone Mineral Density In Childhood Eczema

Dr. Ting Fan Leung, MD, FRCPCH, FAAAAI, Ms. Flora Yin-ying

Kwok, MPhil, Dr. Yvonne Yi-fong Ho, MPhil, Dr. Susan Shuxin

Wang, PhD, Ms. Patty Pui-pui Tse, MSc, Prof. Gary Wing-kin

Wong, MD, FRCPC, Dr. Kam Lun Ellis Hon, MD, FAAP; Department

of Paediatrics, The Chinese University of Hong Kong, Hong Kong.

RATIONALE: Food avoidance is common among eczema children, but

there is limited data regarding effects of such practice on bone mineral

density (BMD). This study evaluated nutrient intakes and BMD in Hong

Kong eczema children.

METHODS: Chinese children with and without eczema were recruited

from allergy or dermatology clinics. Their nutrient intakes were assessed

by food frequency questionnaire and analyzed using Foodworks

Professional software (Xyris, Brisbane, Australia). BMD was measured

atmid-point of radius in the non-dominant arm and left tibia by quantitative

ultrasound bone sonometry (Omnisense 7000P, BeamMed, Tikva, Israel).

RESULTS: 114 eczema children and 60 controls were recruited. Calcium

and vitamin D intakes were lower in moderate-to-severe eczema (50) and

mild (64) eczema than controls (medians for calcium in mg/MJ: 68.1, 74.4

and 89.5, P50.001 for trend; for vitamin D in mg/MJ: 0.10, 0.14 and 0.27,

P50.001 for trend). The mean (SD) BMD z-scores in eczema and controls

were 0.52 (0.90) and 0.55 (1.12) at radius (P50.889) and 0.02 (1.03) and

-0.03 (1.13) at tibia (P50.886). Among eczema children, BMD at radius

was higher in those with first and third tertiles of calcium intake than those

in the second tertile (P50.016). BMD at tibia correlated positively with

calcium intakes (P50.015).

CONCLUSIONS: This study characterizes pattern of dietary restriction

in Hong Kong eczema children, who have low calcium and vitamin D

intakes. Despite this, BMD is similar between eczema and controls. Higher

BMD is found in patients with higher calcium intakes. Funding: Research

Committee Group Research Scheme (3110087) and Direct Grant for

Research (2011.1.058), CUHK