R540 Series

User Manual V1.1

Enhanced Small Animal

Anesthesia Machine

© 2016 Shenzhen RWD Life Science Co., Ltd, All rights reserved.

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The intellectual property rights of this product and its instruction manual belong to RWD Life

Science Co., Ltd (hereinafter referred to as the RWD), including but not limited to patent,

trademark, copyright etc..

RWD reserves final interpretation right of this instruction manual.

RWD have the right to use the instruction as confidential information. Any individual and

organization shall not disclose the instruction of all or parts of the information by any means

without RWD’s written permission. Nor shall any other person or organization be allowed to

obtain all or part of the information of this instruction manual by any means.

No individual and organization shall but not limited to publish, modify, reproduce, issue, rental,

adapted, and translated into other languages with RWD’s writen permission.

is RWD’s registered trademark or mark, these trademarks and the related security

mark belong to RWD’s intangible property. The use of non RWD’s trademark or mark in this

instruction manual are only for editing purposes, without other purposes, the rights belong to their

respective rights owners.

Statement

RWD reserves the right to modify the content of this manual without prior notice.

RWD reserves the right to change the technology without prior notice.

RWD reserves the right to modify the product specifications without prior notice.

RWD is not to guarantee the information in any forms, including (but not limited to) the guarantee

responsibility of proposing the implied merchantability and suitability for a specific purpose.

RWD only in the following conditions is considered to be responsible for the safety, reliability

and performance of the instruments, i.e.:

Assembly operation, expansion, adjustment, improvements and repairs were carried out by RWD

authorized personnel;

Relevant electrical equipments in line with national standards;

The instrument is operated according to the instruction manual.

RWD is not responsible for the products’ safety, reliability and operation status in the following

conditions:

Components are disassembled, stretched and debugged;

Non RWD authorized personnel repairs or alterations to the instruments;

Product using not in accordance with the manual.

CONTENTS 1、INTRODUCTION ................................................................................................. 1

1.1 Overview ................................................................................................................................. 1

1.2 Safety ...................................................................................................................................... 1

1.3 General description ................................................................................................................. 1

1.4 Equipment environment requirements .................................................................................. 2

1.5 Features of main components of equipment ......................................................................... 3

2、Safety ....................................................................................................................... 5

2.1 Overview ............................................................................................................................... 5

2.2 Usage restriction ..................................................................................................................... 5

2.3 Description of safety symbols ................................................................................................. 5

2.4 Basic safety precautions and practices .................................................................................... 6

2.5 System malfunction ................................................................................................................ 7

2.6 Emergency shutdown .............................................................................................................. 7

3、Unpacking and Assembly ...................................................................................... 7

3.1 Overview ............................................................................................................................... 7

3.2 Unpacking ............................................................................................................................. 7

3.3 System assembling ................................................................................................................ 8

4、System Preparation ................................................................................................ 9

4.1 Overview ............................................................................................................................... 9

4.2 Materials and Goods ............................................................................................................... 9

4.3 Initial system preparation ...................................................................................................... 10

5、Operation Guide .................................................................................................. 14

5.1 Overview ............................................................................................................................... 14

5.2 Pre-use checkout ................................................................................................................... 14

5.3 Animal anesthesia procedures ............................................................................................... 14

5.4 Post-anesthesia procedures ................................................................................................. 16

6、Trouble Shooting .................................................................................................. 17

6.1 Overview ............................................................................................................................... 17

6.2 Safety .................................................................................................................................... 17

6.3 Equipment Status .................................................................................................................. 17

6.4 Record keeping ..................................................................................................................... 17

6.5 Problem (fault) - solution summary .................................................................................... 17

7、Maintenance ......................................................................................................... 20

7.1 Overview ............................................................................................................................. 20

7.2 Safety .................................................................................................................................. 20

7.3 Annual inspection ............................................................................................................... 20

7.4 Equipment status ................................................................................................................. 20

7.5 Record keeping ................................................................................................................... 20

7.6 Parts and materials ................................................................................................................ 20

7.7 Equipment Cleaning ............................................................................................................ 21

8、Product Information ............................................................................................ 23

8.1 Overview ............................................................................................................................... 23

8.2 Product warranty ................................................................................................................... 23

8.3 Product features .................................................................................................................... 23

8.4 Product technology specifications ........................................................................................ 24

9、Other Reference Information ............................................................................. 25

9.1 Overview ............................................................................................................................... 25

9.2 Pressure measurement ........................................................................................................... 25

9.3 Pressure unit conversion ..................................................................................................... 25

9.4 Maintain anesthesia concentration reference ...................................................................... 25

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1-CONTENTS

1.1 Overview

First of all, sincerely thanks for selecting the R500 series Small Animal Anesthesia

Machine made by RWD Company.

Please read this instruction manual and all other auxiliary materials carefully before

installing and using the product, which will be helpful to work with it better.

RWD has been always dedicated to improving the product function and the service

quality, and will reserve the rights to revise the product itself and contents described

in the instruction manual at any time without notice in advance.

If you find the practical situations about the supplied goods do not agree with the

contents described in the manual, or have any questions or ideas about our products

and service, welcome to contact us. For latest information, please visit our web site

(www.rwdstco.com) or contact us immediately.

This manual is applicable to:

R540IP Enhanced Small Animal Anesthesia Machine - Isoflurane,Pour Fil

R540IE Enhanced Small Animal Anesthesia Machine - Isoflurane,Easy Fil

R540SP Enhanced Small Animal Anesthesia Machine - Sevoflurane,Pour Fil

R540SE Enhanced Small Animal Anesthesia Machine - Sevoflurane,Easy Fil

1.2 Safety

Operation of Anesthesia System involves oxygen gas with high pressure and the use

of potentially hazardous materials. In order to prevent injury to animals and/or

operators and damage to the anesthesia system, please read carefully Section 2 -

System security before performing any of the procedures contained in this manual.

If you have any safety questions or advises, please contact us for support.

1.3 General description

R540 enhanced anesthesia machines are compact and stable equipments newly

designed by RWD Life Science Co., Ltd. It can withstand daily rigorous test use,

and ensure trouble-free operation for a long time.

The principle is that the anesthetic agent transforms from liquid to gas and mixes

with oxygen according to a certain proportion for inspiration of animals in order to

make them anesthetized.

Note: Because of the different type of RWD produced small animal anesthesia

This small animal anesthesia system should only be operated

and administered by professional personnel.

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machine, its internal structure may also be different, the following principle

description and principle charts just for reference, please carefully read the

content of the instructions for specific parts name and detailed operation.

Figure 1-1 shows a basic working principle diagram of the system. The operator

regulates the valve of the oxygen supply to set the desired flow rate according to the

indication of oxygen meter float. The operator toggles gas switch or the three-way

valve and makes sure one outlet of valve is accessed to the anesthesia induction box,

and sets the concentration of the anesthetic gas (vol%) by turning the knob on the

vaporizer to the desired setting, then puts small animal into the induction box to

start induction anesthesia. After the animal is fully anesthetized, the operator

regulates the concentration of anesthetic gas to maintaining concentration, takes out

the small animal to the end of anesthesia mask, make it to continue inhaling

anesthetic gas, and the exhaled waste gas can be recycled by an optional recovery

unit.

Figure 1-1 Basic working principle diagram of the system

No. Description No. Description

○1 Anesthetic vaporizer ○5 Gas switch

○2 O2 flowmeter ○6 Anesthesia induction box

○3 O2 flowmeter control valve

○7 Anesthesia mask

○4 O2 flush button ○8 Small animal

1.4 Equipment Environment Requirements

Please prepare equipment operation status as Table 1-1 to ensure reliable operation

and safety.

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Table 1-1 Equipment Environment Requirements

1.5 Features of main components of equipment

Figure 1-2 Equipment main body

No. Description No. Description

○1 Handle ○6 Anesthesia induction chamber interface

○2 O2 flowmeter ○7 Gas switch

○3 Anesthetic vaporizer ○8 Anesthesia mask interface

○4 O2 Flow control valve ○9 O2 flush button

○5 Main Suport ○10 Air connecion

Detail Description

Gas Supply Medical oxygen or air pump

Pressure: below 0.4MPa

Working

Environment

Storage temperature：-40 - 55℃

Operating temperature：10-35℃

Relative Humidity：5-90%

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○1 Handle

Provides hand-hold for steadying the anesthesia system while it is being moved.

○2 ○4 O2 flowmeter assembly

Used to control the oxygen or air flow that goes into the anesthesia system. Turn the

O2flowmeter control valve to regulate the oxygen flow between 0.1~4L/min.

○3 Anesthetic vaporizer

The vaporizer is the most core part of the whole anesthesia system. More attention

should be paid to its maintenance and operation.

The vaporizer converts liquid anesthetic into a gaseous state, and add into oxygen in

volume percentage (vol.%). The volume percentage is adjusted by the digital dial

on the upper part of the vaporizer, and the gas out of the outlet is the set

concentration mixed gas

RWD produce and design vaporizer can ensure stable anesthesia gas concentration

output, without influencing by external environment (pressure, temperature,

humidity etc.).

The inner design of the vaporizer is different according to the anesthetic agent.

Please use the correct anesthetic agent following the tag on the vaporizer. The

misuse may cause the damage to the equipment and animal.

○5 Main support

Solid metal support for mounting and supporting all of the above components。

○6 Anesthesia induction chamber interface

Used for connecting animal anesthesia induction chamber.

○7 Gas switch

Pull the gas switch to the direction of the "Chamber", the anesthetic gas flows to the

anesthesia induction chamber. Pull to the direction of the "Mask", the anesthetic gas to

flow to the anesthesia mask.

○8 Anesthesia mask interface

Used for connecting the anesthesia mask.

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○9 Quick flush button.

Press to deliver the oxygen or air directly to induction box. It is generally used

while emergency or excluding residual anesthetic gas in the induction box.

○10 Air connection

Used for connecting oxygen or air pumps.

2- Safety

2.1 Overview

This section provides basic safety information necessary for operating our R540

enhanced small animal anesthesia machine. If you have any questions or advise,

please contact us for more support.

2.2 Usage restriction

RWD R540 enhanced anesthesia machine is only for laboratory or veterinary use. All

the operation and maintenance should follow the instructions of this manual. Misuse

of your system may result in injury to animals and operators and/or damage to

property. Misuse includes:

Using inappropriate gases and anesthetic agents

Making unauthorized modifications to the equipment

Operating the system using gas pressure exceeding maximum ratings

2.3 Description of safety symbols

The following types of symbols are included in this manual with notes that alert the

reader to potential hazards.

Property Damage Caution.This symbol appears in a shaded text block to

warn that your actions could cause damage to the system or the facility.

Personal and Animal Safety Warning. This symbol appears in a shaded text

block to warn that your actions could cause personal injury or death.

This small animal anesthesia system should only be operated

and administered by professional personnel.

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2.4 Basic safety precautions and practices

This section provides requirements necessary to ensure safe and reliable operation of

your R540 enhanced anesthesia machine.

2.4.1 Animal and operator safety

If possible. please prepare a backup set anesthesia machine in case an emergency occur;

Any person responsible for the installation or operation of the system shall carefully

read and familiar with the instruction manual.

To ensure proper function of your system, perform a pre-use checkout procedure in

accordance with Section 5 - Operation.

The system must always be attended by a veterinarian or trained anesthesia technician.

After guarantee period, annual service inspections to ensure proper operation is

recommended. Only RWD-certified technicians should be permitted to service the system

or replace internal parts.

Keep open flames and combustibles (e.g., ether and acetone) away from the anesthesia

system.

Do not place any heavy things on the anesthesia system.

Make sure that the oxygen hose is unobstructed to avoid blockage.

Wearing personal protective equipments is suggested.

Make sure the oxygen hose is securely connected to the system before starting a procedure.

Make sure that any exhaled gas containing anesthetic is vented outside of the operating

room or recycled by other proper equipment.

RWD recommends the use of an electrocardiograph, and equipment capable of monitoring

the animal’s pulse, oxygen (O2) saturation, carbon dioxide (CO2) level, and anesthetic

agent level at all times when operating the system.

Remove the system from service if any indications of improper function exist, and contact

RWD.

Never pour water or any other fluids into the anesthetic vaporizer. Fill vaporizer only with

the anesthetic agent for which it is designed, note the identification outside the vaporizer

before use.

Avoid use of oil or grease on any anesthesia or respiratory equipment where oxygen is

used. An explosion may occur.

Make sure the pressure of the oxygen is below 400kPa。

2.4.2 Preventing system and facility damage

Immediately collect and clean up spilled anesthetic.

Isoflurane is very caustic, avoid it contact with mask and other components if spillage

occurs. If spillage should occur, allow it to evaporate. Do not attempt to wipe it with a

cloth.

Do not place anything on top of the anesthesia equipment.

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Follow all recommended system maintenance procedures specified in Section 7 -

Maintenance.

Keep appropriate distance from the wall to ensure a stable gas supply.

Ensure the stability of gas supply.

2.4.3 Environmental health

Immediately collect and clean up any spilled anesthetic agent.

If the system is used in a confined space, ensure that there is adequate ventilation.

Dispose of any hazardous materials and items contaminated with hazardous materials

should be in accordance with local regulations.

2.5 System malfunction

If your system does not operate properly, refer to Section 6 - Trouble shooting which

includes descriptions, possible causes, and suggested solutions.

2.6 Emergency shutdown

In the event of a major malfunction, please proceed as follow procedures:

1) Turn off the oxygen supply and the vaporizer；

2) Disconnect the line between the equipment and the air source；

3) Switch the animal to a backup anesthesia delivery system and continue the

experiment.；

4) Contact RWD for after-sale service.

3- Unpacking and assembling

3.1 Overview

This section introduces the unpacking and assembly procedures for your R540

anesthesia machine.

3.2 Unpacking

Your RWD R540 enhanced anesthesia machine has been carefully packed in our

factory for safe delivery to you. When you have received your product, please do the

as follow:

Check the shipping documents to ensure that all boxes have been received.

Examine the shipping boxes for damage. Immediately make a damage claim to

the carrier if there is serious damage and contact RWD. Taking photos is

suggested for keeping the evidence.

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Carefully open each box and remove each individual component. Save all boxes

and packing materials for future shipments.

Check the packing list or invoice to ensure all components ordered are included.

In case there is any doubt or need any help, contact RWD or local dealer

immediately.

3.3 Assembling the system

3.3.1 Install Vaporizer

Please refer Figure 3-1、3-2、3-3 install vaporizerr on main support

Figure 3-1 Twist two screw in the vaporizer, do not twist all in

Figure 3-2 Hang the vaporizer on the screw holes of the main frame

Some components of the system are heavy. Use caution while

lifting these components to avoid injury.

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Figure 3-3 Use screwdriver to tighten the screw

3.3.2 Vaporizer line connection

Please refer to Figure 3-4 Connect the tube of the anesthesia machine to the gas

INLET and OUTLET of the vaporizer.

Figure3-4 Vaporizer tube connection

4-System setup

4.1 Overview

This section provides the setup procedures required to prepare your R540 enhanced

animal anesthesia machine.

4.2 Materials and goods

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Oxygen or air source（below 400kPa）

Anesthetics (optional, according to the type of vaporizer)

Anesthesia induction chamber（optional）

Anesthesia mask (optional)

The anesthetic gas recovery system (optional)

4.3 Initial system preparation

4.3.1 Setting up the system

1) Move anesthesia machine to use area

2) Connect the oxygen supply or air pump to the inlet port of anesthesia

machine (shown in 错误！未找到引用源。) and make sure the gas supply is

turned off.

Figure 4-1

3) Make sure the volume percent dial on the anesthetic vaporizer to the zero

(0) position（Figure 4-2）；

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Figure 4-2

4.3.2 Fill in the anesthetic

User choose different vaporizer, the anesthetic filling method is also different.

RWD supply two kinds of filling type vaporizer-Pour Fil and Easy Fil.

4.3.2.1 Pour Fil

1) Turn and remove the sealing cap of the vaporizer. Do not leave any debris.

(Figure4-3)

Figure4-3

If anesthetic agent spills on system surfaces, allow it to evaporate. Rubbing

spilled anesthetic agent with a cloth may damage the system finish.

Make sure to pour the correct anesthetic agent into the filler port. Filling with

the incorrect anesthetic agent could cause serious injury or death to the animal.

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2) Carefully pour the appropriate agent

into the filler port. Keep the agent level

inside the tube between the two marks.

（Figure 4-4）；

Figure 4-4

3) Tighten the sealing cap（Figure 4-5）

Figure 4-4

4.3.2.2 Easy Fil

1) Turn and remove the sealing cap

of the vaporizer. Do not leave

any debris. Check the red

sealing ring.（Figure 4-6）；

Figure 4-6

2) screw dosing adapter on the

anesthetic bottle, and insert it

into the vaporizer inlet and

press in the end（Figure 4-7）；

Figure 4-7

3) Carefully pour the appropriate

agent into the filler port. Observe

the agent level sight glass on the

vaporizer.Keep the agent level

between the two marks(Figure4-8)；

Figure 4-8

4) Tighten the sealing cap（Figure

4-9）.

Figure 4-9

- 13 -

4.3.3 Connecting the breathing tube

Connect the anesthesia induction chamber(optional) and anesthesia mark(optional)

onto the relevant portal of the anesthesia machine.

Figure 4-10 Breathing tube connection

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5- Operation

5.1 Overview

This section introduces how to use the R540 enhanced animal anesthesia machine

to handle animal anesthesia experiments after the preparation works.

5.2 Pre-use checkout

RWD recommend you to check below items before using R540 enhanced animal

anesthesia machine to ensure the stability of the equipment.

Ensure anesthetic agent is filled into the vaporizer.

Make sure the vaporizer volume-percent dial is set to zero (0).

Ensure the oxygen source or air pump is connected with anesthesia system

closely.

Ensure the pressure of air supply is below 400 kPa and it is enough during the

complete experiment.

Make sure the O2 control valve functions normally.

Confirm that the breathing circuit is clear and clean and check the ventilation

and cleanliness of the experimental environment.

5.3 Anesthesia procedures

1) Turn on the oxygen supply or air pump（below 400 kPa）；

2) Turn the O2 control valve counter clockwise rotation and observe the

float position, adjust to suitable flow rate.

Only trained professionals should operate the anesthesia system.

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3) Put animal into the induction chamber, push the air switch to the

direction of Chamber,in the mean time, the anesthesia gas flow to the

induction chamber.

4) Press the dial lock key, rotate the evaporator dial, set the vaporizer

concentration to 4-5% until the animals become immobile. To be sure of

complete anesthesia, animals should remain in the induction chamber for

1 minutes at least after initial induction before moving them to the mask.

Pressing of dial lock key is necessary only when the concentration is set

from position “0”.

5) Reduce the vaporizer setting to the species-appropriate level while the

induction anesthesia is completed.Turn the gas switch to the direction of

“Mask”, in the mean time, the anesthesia gas flow to the anesthesia

masks (the types of the masks may be different)

- 16 -

6) Take out animal from the induction chamber, put its head/nose in the

anesthesia mask (detail procedures depend on your anesthesia mask type),

make sure the animal anesthesia status, and then handle the subsequent

experimental operation.

RWD recommends you to push the quick flush button before taking

out the animal from the anesthesia induction chamber, to exhaust

anesthetic agent in the induction chamber, so as to reduce the anesthetic

gas while opening the induction chamber.

Figure 5-5

5.4 Post-anesthesia procedure

Once the anesthesia procedure is completed, perform the following steps in order:

1) Turn off the vaporizer by turning the volume-percent dial clockwise to

zero (0).

2) Press the oxygen flush button to clean the anesthetic agent in the

induction chamber.

3) Adjust O2 flowmeter valve，until the oxygen flow read zero“0”；

4) Cut off the oxygen supply source;

5) If the system will be vacant for a long time, drain the anesthetic agent

inside the vaporizer as described in Section 7 – Maintenance;

6) Thoroughly clean the anesthesia system as described in Section 7 -

Maintenance.

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6 Troubleshooting

6.1 Overview

This section will help you determine the origin of common problems/alarms you

may experience with your R500 series Anesthesia System and recommended

corrective actions. If you experience problems not listed in this section, or

continue experiencing the problem after trying the suggested corrective actions,

please contact RWD or your local dealer for support.

6.2 Safety

Some troubleshooting procedures may involve the use of hazardous materials and

contact with biological hazards. Always follow all applicable local regulations

and the material manufacturer’s Material Safety Data Sheet (MSDS)

recommendations. During the procedure, basic personal protective is necessary,

such as wearing gloves, mask and eye protection.

6.3 Machine status

Unless otherwise specified, the anesthesia system may be connected to gas

supplies during the performance of the troubleshooting procedures described in

this manual. Make sure the gas supplies and vaporizer are turned off before the

procedure.

6.4 Record keeping

A record of problems and their resolution should be kept. Such records should

include the date, the nature of the problem encountered, and the actions that

resolved the problem.

6.5 Problem – Solution matrix

Table 6-1 contains problems that may occur during operation of your R500 series

Anesthesia System and their corrective actions. If you continue experiencing a

problem after trying the suggested corrective actions, please contact RWD or

local dealer for support.

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Table 6-1 R500 series Anesthesia System: problem - solution matrix

No. Problem Cause Solution

1

No or low

anesthetic

vapor output

a. Vaporizer is

functioning properly,

but machine output is

not getting to animal

Check breathing system

components for leaks, tears, holes,

etc. Ensure the mask/endotracheal

tube makes a good seal with the

animal.

b. Anesthetic agent

reservoir is empty

Fill the reservoir with appropriate

anesthetic agent.

c. Vaporizer is turned

off

Turn the dial on the vaporizer and

adjust it to the desired

volume-percent of anesthetic.

d. Leak around the

vaporizer filler port

Make sure the sealing cap on the

vaporizer is fully closed.

e. Vaporizer

malfunction - internal

fault

Contact RWD or local dealer for

servicing.

2

Animal sleep

level seems too

light

a. Vaporizer is empty Fill vaporizer with the appropriate

anesthetic agent.

b. Anesthetic

concentration is set

too low

Adjust the dial to increase the

volume-percent of anesthetic.

c. Leak in animal

breathing circuit

Check breathing system

components for leaks, tears, holes,

etc. Ensure the mask/endotracheal

tube makes a good seal with the

animal.

d. Leak around the

vaporizer filler port

Make sure the sealing cap on the

vaporizer is fully closed.

3

Animal sleep

level seems too

deep

a. Anesthetic

concentration is set

too high

Adjust the dial to reduce the

volume-percent of anesthetic.

b. Vaporizer

malfunction

Contact RWD or local dealer for

servicing.

4 No gas flow

a. Facility or cylinder

gas supply valve is

closed

Open gas supply valve.

b. Gas cylinder is

empty Replace empty gas cylinder.

c. Gas supply hose is

disconnected

1. Ensure gas supply hose is

connected to the cylinder or

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facility gas system.

2. Ensure the gas supply hose is

securely connected to the

anesthesia system.

d. Oxygen flow

control turned off

Turn the flowmeter valve knob

counterclockwise to increase the

oxygen flow rate.

e. Gas supply

regulator malfunction

Connect the system to an alternate

oxygen supply.

5 Gas flow is not

sufficient

a. Oxygen flow is set

too low

Turn the flowmeter valve knob

counterclockwise to increase the

oxygen flow rate.

b. Leak around

vaporizer inlet port

1. Make sure the sealing cap at the

inlet port is fully closed.

2. Verify that inlet port is not

obstructed by animal hair, etc.

6

Float in oxygen

flowmeter

sticks

Dirty flow tube Flow tube needs cleaning.

7

Oxygen flow

control valve

knob is hard to

turn

Dirty or damaged

needle valve

Flow valve needs cleaning or

replacement. Contact RWD or

local dealer for servicing.

8 Oxygen flow

failure Internal fault

Contact RWD or local dealer for

servicing.

9

Audible

leakage around

oxygen hose

connector

a. Loose oxygen hose

connection

Tighten the connection with a

crescent wrench.

b. Tube does not fit. Replace the tube.

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7 Maintenance

7.1 Overview

This section provides the maintenance procedures to keep your R500 series

Anesthesia System in good operating condition.

7.2 Safety

Some maintenance procedures may involve the use of hazardous materials and

contact with biological hazards. Always follow all applicable local regulations and

the material manufacturer’s Material Safety Data Sheet (MSDS) recommendations.

During the procedure, basic personal protective is necessary, such as wearing

gloves, mask and eye protection.

7.3 Annual inspection

RWD recommends that your R500 series Anesthesia System be inspected annually

for proper function. Contact RWD or your local dealer for service.

7.4 Machine status

Unless otherwise specified, the anesthesia system may be connected to gas supplies

during the performance of the maintenance procedures described in this manual.

However, make sure the oxygen supply is OFF and the vaporizer is set to zero

volume-percent.

7.5 Record keeping

A record of system service and maintenance should be kept. Such records should

include service and maintenance dates, part numbers of any replaced parts, dates

when consumables are replenished, and other pertinent data.

7.6 Parts and materials

Contact RWD or your local dealer if parts or materials are needed during the

maintenance.

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7.7 Cleaning the equipment

7.7.1 Required materials and supplies

Germicidal cleaner

Water and clean cloth

Personal Protective Equipment (masks, eye protection, gloves, etc.)

7.7.2 Weekly maintenance

Wipe all surfaces of the system with a cloth dampened with germicidal

cleaning solution.

Remove hair, dust, and debris from all vaporizer surfaces; especially around

the volume-percent dial and the anesthetic fill port.

Check the weight of gas filter canister, replace it if more than 1000 g.

Perform a pre-use checkout as specified in Section 5 - Operation.

Check the cleanliness of the oxygen supply.

7.7.3 Draining the vaporizer

If the system will be vacant for a long time, drain the anesthetic agent inside the

vaporizer.

1) Make sure the oxygen or air supply is OFF and the vaporizer is set to zero

volume-percent.

2) Make sure the anesthesia system is in a well-ventilated area.

Do not wipe spilled anesthetic agent from any painted surfaces, otherwise

the finish may be damaged. Allow the anesthetic agent to evaporate.

Perform this procedure in a well-ventilated location.

Refer to the anesthetic agent manufacturer’s MSDS for required

personal protective equipment and handling and disposal of waste

anesthetic agent.

Do not mix the anesthetic agent with other liquid.

Maintenance of the anesthesia system involves possible contact with

biological and chemical hazards. Wear gloves, mask, and eye protection

during all cleaning

procedures.

- 22 -

3) Attach a drain tube to the drain port. Place the other end of the tube in a

receptacle to catch the drained anesthetic agent.

4) Loosen the sealing cap, then loosen the bottom screw with a screwdriver to

make the anesthetic agent flows naturally.

5) When the anesthetic agent stops draining from the vaporizer, tighten the

bottom screw and sealing cap.

6) Remove the drain tube and deal with the waste anesthetic agent according to

the regulations.

7) If the anesthesia system is vacant more than 6 months, use oxygen to flush

the vaporizer thoroughly.

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8 Product information

8.1 Overview

This section provides the warranty information, features and specifications of R500

series Anesthesia System.

8.2 Product warranty

This warranty is only applicable to those new products purchased from RWD or

dealers authorized by RWD, as well as the first person to whom it extends.Within

one year from the invoice date of the original purchase, the defects in material and

workmanship are fee to be repaired and replaced by RWD.

All conditions of this warranty become null and void if the equipment is abused and

misused or is repaired and replaced by companies other than RWD.

RWD should not be responsible for any damages of product due to the accidental,

indirect damage and special operation.

8.3 Product features

Standard open circuit breathing loop design.

Applicable to rats, mice, rabbits, cats, hamsters and other species.

Single breathing circuit design, non-breathing circuit system.

Unipue pipe design inside, ensure the output of anesthetic gas without effect by

pressure variations.

The oxygen flow meter adjustable ranges from 0 to 1000 ml/min.

Oxygen or air is alternative as the gas source.

Pour Fil style anesthetic vaporizer, with capacity ≥ 120 ml, automatic flow and

temperature compensation.

The concentration of anesthetic gas from the vaporizer can be adjusted(0 -

5%,Isoflurane;0-8%,Sevoflurane） , without effect by fluctuation of flux,

temperature, flow rate and pressure. The safety locking device well prevents

accidental volatilization of anesthetic agent.

The optional multi-channel component meets the induction and maintenance

anesthesia for up to 5 animals.

With the locking structure for preventing accidental stating and protection

structure in closed state, ensure the system security.

Accessories with various specifications (induction box, mask, anesthetic gas

recovery system etc,) will meet the need of animal with different species and

size.

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8.4 Product specifications

Material Mainly aluminum alloy

Working condition Temperature：10 ~ 35 °C Humidity：5 ~90%

Storage condition Temperature：-40 ~ 55 °C Humidity：5 ~ 90%

Oxygen flow rate 0.2~15 L/min, 0.2~8 L/min when concentration >4%

Oxygen grade Medical grade or air

Concentration range Isoflurane:0.2～5% (v/v); Sevoflurane: 0.2～8% (v/v)

Settable concentration

point

Isoflurane:0 ~ 0.2 ~ 0.5 ~ 1.0 ~ 1.5 ~ 2.0 ~ 2.5 ~ 3.0 ~ 3.5 ~

4.0 ~ 4.5 ~ 5.0%(v/v)

Sevoflurane: 0 ~ 0.2 ~ 0.5 ~ 1.0 ~ 1.5 ~ 2.0 ~ 2.5 ~ 3.0 ~ 3.5 ~

4.0 ~ 4.5 ~ 5.0 ~ 6.0 ~ 7.0 ~ 8.0%(v/v)

Accuracy of concentration

control ±5% of full scale or ±15% of set value

Perfusion volume of

anesthetic agent

The volume between max and min visible liquid level is about

120mL.

Consumption of

anesthetic agent

About 3 × oxygen flow rate (L/min) × set concentration

value(%(v/v))

e.g. When the Isoflurane concentration is set at 2% and the

oxygen flow rate is set at 600 mL/min, a bottle of Isoflurane of

100 mL could be consumed for 28~30 hours.

Loss of anesthetic agent 22 °C, dial at 0%, <0.5 mL/24 h.

Max inclined angle 30°

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9 Useful information

9.1 Overview

This section provides information that may be helpful to the users of R500 series

Anesthesia Systems.

9.2 Pressure equivalents

1 atm = 1033 cmH2O = 760 mmHg = 760 Torr = 1013 mb = 14.7 psi

1 psi = 70.3 cmH2O = 51.7 mmHg = 68.9 mb = 6.9 kPa

1 mmHg = 1.36 cmH2O = 1.33 mb

1 cmH2O = 0.736 mmHg = 0.981 mb

9.3 Pressure unit conversions

Table 9-1 Pressure unit conversions

Unit psi inchH2O kPa millibar cmH2O mmHg

psi 27.680 6.8947 68.947 70.308 51.715

inch H2O 3.6127x10-2

0.2491 2.491 2.5400 1.8683

kPa 0.14504 4.0147 10.000 10.1973 7.5006

millibar 0.01450 0.40147 0.100 1.01973 0.75006

cmH2O 1.4223x10-2

0.3937 0.09806 0.9806 0.7355

mmHg 1.9337x10-2

0.53525 0.13332 1.3332 1.3595

RWD Life Science

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Add: No.11 Hi Tech North Rd, Nan Shan Dist., Shenzhen, P.R.China

Tel: 001-858-900-6602 86-755-86111286

Email: sales@rwdstco.com

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