Question 1 1 / 1 point

Figure 1: Plasma membrane labeled with RFP, protein tagged with GFP

Where in the cell are the GFP tagged proteins localized?

Mitochondria

Cytosol

Golgi

RER

Question 2 1 / 1 point

Figure 2. Nuclei are stained with DAPI, secreted protein tagged with GFP

Where in the cell are the GFP-tagged proteins localized?

Golgi

Cytosol

Nucleus

RER

Question 3 1 / 1 pointA mutated PTS1 (Ser-Lys-Leu) targeting sequence on a catalase protein would result in....

Inability for the protein to be transported into the peroxisome

Inability for the protein to be embedded in the peroxisomal membrane

The protein to be transported into the peroxisome, but become non-functional

No change in the proteins normal activity

Targeting of the protein to the mitochondria

Question 4

1 / 1 point

The mitochondiral matrix targetting motif is...

Necessary, but not sufficient

Sufficient, but not necessary

Necessary and sufficient

Neither necessary nor sufficient

question set motifs

Question 5 1 / 1 pointA protein that has a serine-lysine-leucine motif at its C-terminus will likely end up in which organelle?

Peroxisome

Mitochondria

Nucleus

ER

Chloroplast

Question 6 1 / 1 pointA protein that has a motif rich in valines, threonines, and serines at its N-terminus will likely end up in which organelle?

Peroxisome

Mitochondria

Nucleus

ER

Chloroplast

Question 7 1 / 1 point

Researchers have the DNA for a new protein. They do some investigating and find that the DNA sequence is similar to a known mitochondrial protein in mouse. They want to determine if this new protein is also a localized to the mitochondria in humans. They decide to create a DNA construct which, when translated, will create a fusion protein with their new protein and GFP. They also purchase from a supplier a RFP (red fluorescence protein) with the mitochondrial matrix targetting motif at its N-terminus. They introduce these two proteins into cultured HeLa (human cancer cells).

Based on their hypothesis, they expect to see red and green in the same areas of the cell.

Based on their hypothesis, they expect to see red and green in different areas of the cell.

question set mito expQuestion 8 0 / 1 point

The images the researchers obtained from the experiment described in Q7 are shown here. What can they conclude?

Their protein is not targetted to the mitochondria.

Their protein is targetted to the mitochondia, but there is not enough evidence to indicate where in the mitochondria it is localized.

Their protein is targetted to the mitochondial matrix.

Their protein is targetted to the inner membrane of the mitochondia.

Their protein is targetted to the inter-membrane space of the mitochondia.

Their protein is targetted to the outer membrane space of the mitochondia.

Question 9

1 / 1 point

In the video presentation on mitochondrial targeting Dr. Lithgow described protein complexes in the mitochondrial membranes that "direct traffic" into the various parts of the mitochondria. Which of the following is not one of the complexes named.

TIMbit

SAM

TIM22

TOM

Lithgow setQuestion 10 1 / 1 point

In the video presentation on protein import to the mitochondria Dr. Lithgow described protein complexes in the mitochondrial membranes that "direct traffic" to the various parts of the mitochondria. To reach the inner membrane a protein must go through which sequence.

through the TOM complex, to tiny TIMs, to the TIM22 complex.

through the TOM complex, to tiny TIMs to the SAM complex.

through the TOM complex to the TIM23 complex, then MPP cleaves the target sequence.

through the TOM complex, to the TIM23 complex, then get processed by IMP.

question set locationQuestion 11 0 / 1 pointA researcher has added an amphipathic alpha-helix motif to the C-terminus of GFP, which is normally a cytoplasmic protein. Where would you hypothesize this protein might end up in the cell?

Cytoplasm

Mitochondria

Nucleus

ER

Peroxisome

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Check which end of the polypeptide the signal sequence needs to be!

question setQuestion 12 0 / 1 pointA researcher adds the mitochondrial hydrophobic stop-transfer sequence to GFP. Where would you expect to see GFP when visualizing with fluorescence microscopy?

In the cytosol

In the mitochondrial matrix

In the inner membrane of the mitochondria

In the inter-membrane space of the mitochondria

In the outer membrane of the mitochondria

palade questionQuestion 13 0 / 1 point

From the video lecture by Dr. Nicchitta on the endoplasmic reticulum the experiments of Jamieson and Palade showed movement of radiolabelled proteins through the cell. In this experiment, called a pulse-chase, they determined the path these proteins take to be:

From the nucleus to the RER, to the Golgi to the secretory vesicles.

From the RER to the peripheral Golgi, to the condensing vacuoles of the Golgi, to secretory vesicles.

From the RER to the condensing vacuoles of the Golgi, to the peripheral Golgi, to the mitochondria.

From the RER to the condensing vacuoles of the Golgi, to the peripheral Golgi, to secretory vesicles.

Question 14

1 / 1 point

From the video lecture by Dr. Nicchitta, the experiments of Blobel and Sabatini showed that:

secreted proteins were synthesized in the cytosol and transported to the ER.

secreted proteins were susceptible to protease during synthesis

the RER is so named because it is free of ribosomes.

secreted proteins were synthesized at the RER and passed into the lumen of the ER.

Attempt Score:

10 / 14

(71.43 %)

Overall Grade (highest attempt):

10 / 14

(71.43 %)

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