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Quantification des structures 3D à partir de coupes histologiques sériées R. Kraftsik. Programmes disponibles : Neurolucida - PC structures arborescentes, coupes sériée. Volume - SGI Indigo + G.T. coupes sériées. Neurolucida - PowerPoint PPT Presentation
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Quantification des structures 3D à partir de coupes histologiques
sériées
R. Kraftsik.
Programmes disponibles :Neurolucida - PC
structures arborescentes, coupes sériée
Volume - SGI Indigo + G.T.coupes sériées
Neurolucida
• Neuron Tracing: Trace and analyze branching neuron processes.
• Brain Mapping: Map, count cells, and measure their locations and morphology
• Morphometry: Make accurate anatomical maps and measurements of histologically distinct structures and regions
Neurolucida system:
-Neurolucida Software -Image Overlay Device
(Optical and/or Video based) -Microscope Stage Control Device -Tracing Device -Graphics Output Device -PC Computer System.
Neurolucida system, room 127
- color camera 640 X 480
- stepping stage + controller
- Zeiss microscope, light and fluorescence
Contact:
R. Kraftsik
FEATURES: - Accurately overlays a computer
graphics or text display onto the microscope image
- High brightness intensity - Up to 1024x668 pixels resolution (PC
and MAC) - Accessible controls for contrast and
brightness - Does not interfere with conventional
use of the microscope
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DRG - CR
Sciatic nerve
infra-granular cells supra-granular cells
Adult ferret
Volume Program, SGI
Contact: R. Kraftsik
Volume program, SGI. Contact R. Kraftsik
Reconstruction of cortical neurons from EM sections
Volume program, SGI. Contact R. Kraftsik
Reconstruction of dendritic segments from EM sections