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QUALITY ASSURANCE
PROJECT PLAN
For
Baseline Water Quality Assessment for Levelock, Alaska
Prepared by
Levelock Village Council Tribal Environmental Program
P.O. Box 70, Levelock, Alaska 99625 Phone: (907) 287-3067 Fax: (907) 287-3167
E-mail: [email protected]
with assistance from
Zender Environmental Science and Planning Services 308 G Street, Suite 312, Anchorage, AK 99501
Prepared for
U.S. Environmental Protection Agency Region 10
and
State of Alaska
Department of Environmental Conservation
Final
January 2007
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A2. TABLE OF CONTENTS ELEMENT SECTION PAGE
PROJECT MANAGEMENT:
A1 TITLE AND APPROVAL PAGE 2
ACKNOWLEDGEMENTS 2
A2 TABLE OF CONTENTS 3
A3 DISTRIBUTION LIST 5
A4 PROJECT TASK / ORGANIZATION 6
A5 PROBLEM DEFINITION/ BACKGROUND 8
A6 PROJECT / TASK DESCRIPTION 9
A7 DATA QUALITY OBJECTIVES FOR MEASUREMENT DATA 10
A8 TRAINING REQUIREMENTS/CERTIFICATIONS 13
A9 DOCUMENTATION AND RECORDS 13
MEASUREMENT / DATA ACQUISITION:
B10 SAMPLING PROCESS DESIGN 16
B11 SAMPLING METHODS REQUIREMENTS 17
B12 SAMPLE HANDLING AND CUSTODY REQUIREMENTS 18
B13 ANALYTICAL METHODS REQUIREMENTS 18
B14 QUALITY CONTROL REQUIREMENTS 19
B15 INSTRUMENT / EQUIPMENT TESTING, INSPECTION, AND MAINTAINENCE REQUIREMENTS
19
B16 INSTRUMENT CALIBRATION AND FREQUENCY 19
B17 INSPECTION / ACCEPTANCE OF SUPPLIES AND CONSUMABLES 20
B18 DATA ACQUISITION REQUIREMENTS 20
B19 DATA MANAGEMENT 20
ASSESSMENT AND OVERSIGHT:
C20 ASSESSMENT AND RESPONSE ACTIONS 21
C21 REPORTS TO MANAGEMENT 21
DATA VALIDATION AND USABILITY:
D22 DATA REVIEW, VALIDATION AND VERIFICATION 21
D23 VALIDATION AND VERIFICATION METHODS 21
D24 RECONCILIATION WITH DATA QUALITY OBJECTIVES 22
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ELEMENT SECTION PAGE
REFERENCES 22
FIGURES
F1 ORGANIZATIONAL CHART 6
TABLES
T1 ANNUAL SCHEDULE OF TASKS 10
T2 DATA QUALITY OBJECTIVES AND METHOD REFERENCES 14
T3 SAMPLING SITES AND GPS COORDINATES 16
T4 SAMPLE PARAMETERS, METHODS, CONTAINER SIZE, PRESERVATIVES AND HOLD TIMES
18
APPENDICES
A FUTURE SAMPLING SITES AND PARAMETER METHOD ADDITIONS 24
B INSTRUMENT CALIBRATION/INSPECTION PROCEDURES AND RECORDKEEPING
26
C SAMPLE ANALYSIS AND COLLECTION PROCEDURES 32
D ICP-MS SCAN DETECTION AND REPORTING LIMITS 35
E MAP OF SAMPLING AREAS 36
F DATA COLLECTION FIELD SHEET 38
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A3. DISTRIBUTION LIST Bristol Bay Native Association Susan Flensburg, Environmental Program Manager P.O. Box 310 Dillingham, AK 99576 Tel: (907) 842-5257 Nondalton Tribal Council Charlotte Balluta, IGAP Coordinator PO Box 49 Nondalton, AK 99640 Tel: (907) 294-2288 Pedro Bay Village Council Ben Foss, Environmental Coordinator PO Box 47020 Pedro Bay, AK 99644 Tel: (907) 850-2342 Kokhanok Village Council Tammy Mann, Environmental Coordinator PO Box 1007 Kokhanok, AK 99606 Tel: (907) 282-2202 Newhalen Tribal Council Ray Wassillie, IGAP/EPA Coordinator PO Box 207 Newhalen AK 99606 Tel: (907) 571-1720 Igiugig Tribal Village Council Roy Andrew, IGAP Coordinator PO Box 4008 Igiugig, AK 99613 Tel: (907) 533-3211 Lake & Peninsula Borough Marv Smith, Community Development Coordinator P.O. Box 495 King Salmon, AK 99613 Tel: (907) 246-3421 Lake Clark National Park and Preserve John Branson Park Historian 1 Park Road Port Alsworth, AK 99653 Tel: (907) 271-3751 Dan Young, Fisheries Biologist Tel: (907) 781-2113 Alaska Department of Fish & Game Division of Subsistence Ted Krieg, Subsistence Resource Specialist PO BOX 1030 Dillingam, AK 99576-1030 Tel: (907) 842-5925 Davin Holin, Subsistence Resource Specialist Tel: (907) 267-2807 US Fish and Wildlife Service Office of Subsistence Management 3601 C Street, Suite 1030, Anchorage, AK 99503 Tel: (907) 786-3888
Lynn Zender, Principal Lisa Ferber, Environmental Scientist Zender Environmental Science and Planning Services 308 G Street, Suite 312 Anchorage, AK 99501 Tel: (907) 277-2111 Include contact information of granting agency, foundation, or organization that funds this work. TECHNICAL RESOURCES Forest Taylor, Laboratory Project Manager SGS Environmental Services 200 W. Potter Drive Anchorage, AK 99518 Tel: (907) 562-2343 Dan Bogan, Bioassessment Project Manager UAA-Environment and Natural Resources Institute 707 A Street Anchorage, AK 99501 Tel: (907) 257-2744 Courtenay Carty, QA Officer/Science Advisor Natural Resources-Environmental Specialist Bristol Bay Native Association P.O. Box 310 Dillingham, AK 99576 Tel: (907) 842-5257 Mark Jen, IGAP Project Officer US EPA Alaska Tribal IGAP Program 222 West 7th Ave. #19 Anchorage, AK 99513 Tel: (907) 271-6324 Jim Gendron, ADEC QA Officer 410 Willoughby Ave, Suite 303 Juneau, AK 99801 Tel: (907) 465-5305 Roy Araki, US EPA QA Officer US EPA Region 10 1200 Sixth Avenue (MailStop OEA-095) Seattle, WA 98101 Tel: (206) 553-6395
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A4. PROJECT ORGANIZATION
Figure F1
Project Coordinator S ndrew L Village
cil
Science Advisor/ Project QA Officer
Courtenay Carty Bristol Bay Native Association
W uality T ians
(To b
US EPA QA OfficerRoy Araki
US EPA Region 10
Laboratory Project Manager Forest Taylor
SGS Environmental
Bioassessment Project ManagerDan Bogan
US EPA IGAP Project OfficerMark Jen
Alaska Tribal IGAP Program
Local Community EldersPeter Apokedak, Sr. Nick Apokedak, Sr.
ADEC QA Officer Jim Gendron
ADEC
hirley Aevelock
Coun
ater Qechnic
6
e named by LVC)
UAA-ENRI
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Responsibilities Community Resources Peter Apokedak, Sr. and Nick Apokedak, Sr. Local Community Elders The main responsibility of the local community elder shall include providing historical and cultural information about the water resources that is being monitored based on their observations and experiences with the water resources and surrounding natural environment. Elders will assist the tribe in making decisions that will affect the tribe’s cultural heritage. Shirley Andrew, Project Coordinator Maintains and organizes water quality training and recertification records for water quality technicians and self. Supervises water quality technicians. Maintains inventory of supplies and replaces as necessary. Manages project data by importing into Microsoft Excel. Collects baseline data with assistance from field samplers. Reports water quality information to the Council, community, and to EPA on an annual basis. Water Quality Technicians (to be named by Levelock Village Council) Participates in water quality training and annual recertification for data collection. Prepares for and initiates data collection events. Responsible for bringing watershed/water quality issues identified during sampling to the attention of the project coordinator and/or Levelock Village Council. Caretakers of community sampling equipment and supplies. Performs other tasks as directed by the Project Coordinator Technical Resources Forest Taylor, Laboratory Project Manager, SGS Environmental Services Provides data results to project coordinator in a timely manner. Reports any hold time exceedences or irregularities of data. Dan Bogan, Bioassessment Project Manager, UAA-Environment and Natural Resources Institute Provides benthic macroinvertebrate sample results and brief interpretation to project coordinator in electronic format. Supplies project coordinator with preserved specimens for educational purposes. Courtenay Carty, Project Quality Assurance Officer Works with project coordinator to ensure QAPP addresses project objectives and community needs. Assists project coordinator in obtaining water quality training and technical assistance. Works with project coordinator to ensure that data meet data quality objectives. Reviews assessment program, training procedures, and data collected on an annual basis. Mark Jen, IGAP Project Officer, US EPA Alaska Tribal IGAP Program Provides direction regarding IGAP program requirements and allowable grant expenditures.
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Jim Gendron, ADEC Quality Assurance Officer Provides guidance regarding QAPP requirements and is responsible for its approval. Roy Araki, US EPA Quality Assurance Officer, US EPA Region 10 Provides guidance regarding QAPP requirements and is responsible for its approval. A5. PROBLEM DEFINITION/BACKGROUND Background The Levelock Village Council is a federally-recognized Tribe located on the Kvichak River in Southwest Alaska, approximately ten miles inland from Kvichak Bay. The Kvichak River drains Lake Iliamna, the largest lake in Alaska, and is generally ice-free from June to mid-November. The river intersects the mouth of the Alagnak (Branch) River about ten miles south of Levelock. Levelock has a primarily maritime climate, receiving an average of 26 inches of rain and 70 inches of snow per year. Subsistence and commercial fishing activities are mainstays for residents of Levelock (ADCA, 2006). Protecting the excellent water quality of the waters surrounding Levelock is a high priority for the community. Water quality studies have been carried out in nearby Lake Clark and Katmai National Parks, both of which have areas that drain into the Kvichak River (e.g., NPS, 1997; Weeks, 1999; others). Recently, extensive baseline environmental studies of hydrology, subsistence, fish, and other environmental aspects have been conducted north of Lake Iliamna in an area known as the “Pebble Deposit”. Northern Dynasty Minerals is pursuing development of an open pit mine at this site, which is a large, copper-gold-molybdenum porphyry deposit located near the headwaters of several anadromous streams. The project is currently preparing to apply for permits from the Alaska Department of Natural Resources. A number of studies have been carried out by agencies and organizations on the Kvichak and/or Alagnak Rivers regarding fish distribution, salmon management, and subsistence harvesting (e.g., Morris, 1985; Collins and Dye, 2003; Fair, 2003; others). One such study by researchers from the University of Alaska Fairbanks assessed bioaccumulation of mercury in salmon on the Kvichak and other rivers in Western Alaska. The study found that Alaska salmon do not exceed the critical human consumption value of 200 ng/g, and that glacial-fed and non-glacial-fed rivers had mercury concentrations below the Alaska state drinking water standard of 2 µg/L (average of 26.4 and 2.0 ng/L respectively) (Duffy and Zhang, 2001). The U.S. Geological Survey measured a suite of water quality parameters thirteen times on the Kvichak River near Igiugig (Site 15300500) between 1957 and 1972. The USGS also measured stream discharge between 1967 and 1987 at this site (USGS, 2006). Using the USGS stream discharge data, the Alaska Department of Fish and Game filed an instream flow reservation for the Kvichak River in July 2003, which is awaiting adjudication from the Alaska Department of Natural Resources (Estes, 1998; Tom Cappiello, ADF&G Fisheries Biologist, oral commun., 2006). Water quality was measured at several locations on the Alagnak River as part of another USGS study that focused on channel stability of the river (Curran, 2003). The study found the Alagnak
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River to be a “nutrient poor, calcium-bicarbonate water with low suspended sediment concentrations” (Curran, 2003). No adverse human impacts on water quality were detected at three sites sampled along the river, though the study was not designed to measure localized impacts (Curran, 2003). While a number of studies have been completed in our region, we wish to perform a water quality assessment at specific sites that are important to us for subsistence purposes. We can then monitor the quality of our water and better understand any changes that occur in the near future or in the years to come. A6. PROJECT/TASK DESCRIPTIONS The primary focus of our sampling plan is to perform a baseline water quality assessment of the rivers and streams to determine and document the health of the Lake Iliamna watershed near Levelock. We will sample monthly in summer (total of three sampling events per year) for the following field parameters: air temperature, water temperature, dissolved oxygen, pH, specific conductance, turbidity, E. Coli, and total coliform. In addition, samples will be sent to a for heavy metals analysis. Benthic macroinvertebrate samples will be collected twice per year at Site 2 (Yellow Creek). The water quality results will be compared to Alaska DEC and EPA water quality standards. Objectives The objectives of the Levelock baseline water quality assessment project are:
• To assess impacts from present activities • To develop a baseline water quality data set to protect water from future impacts • To screen water quality data for any adverse impacts to water quality.
River and stream surface water samples will be collected at low tide, at elbow-depth, and at the point of highest flow (likely midstream) at three locations (see Section B10—Sampling Site Selection). The Levelock Village Council has selected additional sites and sampling methods (Appendix A) that may to be added to our assessment project as time and funding allow. Any changes to this plan will be submitted to ADEC and EPA as an addendum. The annual schedule of tasks for this project is listed in Table T1. The parameters measured, methods used, and method references for this project are listed in Table T2. All personnel will follow the instrument calibration and recordkeeping procedures (Appendix B) as well as sample analysis and collection procedures (Appendix C). The Levelock Water Quality Assessment Project is an on-going project designed to continue as long as funding allows. We hope to collect water quality data for at least three years.
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Table T1 SCHEDULE OF BASELINE ASSESSMENT EVENTS
MAJOR TASK CATEGORIES J F M A M J J A S O N D
Inventory supplies, order as needed
X
Training/Re-certification X
Water quality testing & equipment calibration checks
X X X X
Macroinvertebrate sample collection
X X
Data entry X X X X
Data validation X X
Annual report X
A7. DATA QUALITY OBJECTIVES FOR MEASUREMENT DATA Objectives for precision, accuracy, representativeness, comparability, and completeness are summarized below, and Table T2 lists the data quality objectives for this project. The sample matrix for all parameters is surface water except air temperature (air) and macroinvertebrates (biota). These data quality objectives (DQOs) were selected to support the project objectives as detailed in Section A6. Precision Precision is the degree of agreement among repeated measurements of the same characteristic, or parameter, and gives information about the consistency of methods. The project coordinator will perform duplicate sample analyses during all sample collection events and annual quality control (QC) sessions. Variation of duplicate values for each parameter must not exceed the range of precision specified in Table T2. If the precision range is exceeded, a third measurement will be taken. Relative percent difference between duplicate samples will be calculated for all data, and coefficient of variation (CV) will be calculated for triplicate samples. Field analytical precision will be evaluated by the relative percent differences (RPD) between field duplicate samples using the following formula:
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RPD = (R1 - R2) x 100 ((R1 + R2)/2)
Where: R1 = the larger of the two replicate values R2 = the smaller of the two replicate values Coefficient of Variation (CV, triplicate samples) is defined: CV=(S/Xbar) x 100, where s = standard deviation of repeated samples and Xbar = mean of repeated samples. Field accuracy will be routinely checked according to the instrument and analytical method accuracy requirements (see Table T2 - Data Quality Objectives) of each parameter. Commercial laboratory Accuracy and Precision: QC samples for accuracy and precision in a laboratory setting may include the analysis of the following: duplicate samples, laboratory control check and laboratory control check duplicate samples (LCS/LCSD) and/or matrix spike and matrix spike duplicate (MS/MSD) sample analyses and addition of surrogate spike. LCS and LCSD analyses are blank samples (from the lab) injected (spiked) with a known concentration of target compounds processed on the same date as the routine samples and analyzed with the routine samples. LCS/LCSD are usually performed in cases where insufficient amount of routine samples are available for the MS/MSD QC analyses. MS/MSD analyses are routine samples injected with a known concentration of target compounds processed on the same date and same way and analyzed with the routine samples. Surrogate spike is a compound that is not one of the target compounds but belongs to the same chemical category and has the same characteristics as the target compounds. Accuracy are determined by calculating the recoveries (%R) of the target compounds spiked into the LCS/LCSD and/or MS/MSD samples or the surrogate spiked into the sample using the following formula: % R = SQ – NS x 100 spike where: % R = percent recovery SQ = the concentration of the spiked compound measured in the routine or blank sample NS = concentration of the target compound native to the unspiked routine or blank sample Spike = the concentration of the target compound spiked in the routine or blank sample Laboratory precision values are calculated as follows: RPD = (R1 - R2) x 100 ((R1 + R2)/2) where: RPD= Relative Percent Difference and R1 and R2 are the initial and duplicate measurement values, respectively
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in case of MS/MSD and/or LCSD/LCSD
R1 = % Recovery of the target compound in the initial analysis (from MS or LCS) R2 = % Recovery of the target compound in duplicate analysis (from MSD or LCSD)
Accuracy
Accuracy is a measure of confidence that describes how close a measurement is to its “true” value. In this program, accuracy is measured by comparative sampling. Values must fall within the specific range for each parameter as indicated in Table T2. Accuracy of equipment used in the assessment program will be verified using standard testing materials for pH, conductivity, and turbidity. Calibration methods are listed in Table T2 and described in further detail in Section B16 and Appendix A.
Representativeness
Representativeness is the extent to which measurements represent the true environmental condition. It is the degree to which the data from the project accurately represent a particular characteristic in a watershed. Representativeness of data is considered in project design and sampling site selection. Representativeness will not be routinely monitored throughout the project, but is incorporated when necessary in interpreting the data.
Comparability
Comparability is the degree to which data can be compared directly to similar studies. Using standardized sampling, analytical methods and units of reporting with comparable sensitivity helps ensure comparability. All testing methods are EPA-approved and/or currently being employed by other water-quality assessment programs throughout the state.
Completeness
Completeness is the comparison between the amount of usable data collected versus the amount of data called for in the sampling plan. In this assessment program, completeness is measured as the percentage of total samples collected and analyzed as a whole and for individual parameters and sites as compared to the goals set out by the project design. Percent completeness is calculated using the following formula:
% Completeness (per parameter) = # of valid results x 100 # of samples taken We recognize that due to unforeseen circumstances (weather, illness, wildlife, fire, etc.), one of our five sampling events may need to be delayed, partially completed, or canceled. We therefore are setting a minimum completeness goal of 80% for our assessment.
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A8. Special Training/Certifications The project coordinator and water quality technicians will receive training prior to collecting baseline water quality data. We plan to secure funding to either attend a certifiable water quality training held by another organization in Alaska (Soil and Water Conservation District, Citizen’s Environmental Monitoring Program, or other) or to hire an instructor to provide a certifiable water quality training in Levelock. If the training is held in Levelock, it will coincide with the first sampling event. Project staff will be re-certified annually. A9. Documentation and Records All data will be recorded on site at the time of sampling using the data sheet, which will be filled out completely and legibly. The comment section of the data sheet will be used to report any problems or abnormalities with sampling procedures or equipment. The equipment calibration and maintenance information will be entered into the appropriate log (Appendix B) at the time of calibration/maintenance, which will occur minimally at the beginning of each day that sampling is performed. Digital photographs of sample sites will be kept on the project coordinator’s computer and backed up on CD. All documents will be kept on file at the project coordinator’s office for a minimum of 10 years. The expected turnaround time for macroinvertebrate results from UAA-ENRI is six to eight weeks.
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Table T2: DATA QUALITY OBJECTIVES AND METHOD REFERENCES (Sample matrix is water for all samples except air temperature) Field Parameter
Method/Range Units Precision Accuracy CalibrationMethod
EPA Method Reference
Thermometer –5.0 + 50.0 °C
Degrees Celsius (°C)
±1.0 °C ±0.5 °C NIST Certified Thermometer
170.1 Temperature
Hanna Combo Meter (HI 98129) 0 to 40 °C
Degrees Celsius (°C)
±0.5 °C ±0.5 °C NIST Certified Thermometer
170.1
pH Hanna ComboMeter
Standard pH units (at 20°C)
(HI 98129) 0.0 to 14.0
±0.3 units ±0.01 units Standard Solutions Method
150.1
Specific Conductance
Hanna Combo Meter (HI 98129) 0 to 3999 µS/cm
µS/cm (converted to 25 °C)
2% of full scale (80 µS/cm) 2% of full scale (80 µS/cm)
Standard Solutions Method
120.1
Dissolved Oxygen
Colorimetric (CHEMetrics kit K-7512)
Parts per million (ppm)
+ 1 ppm Accuracy: + 0.5 ppm (1-6 ppm) and + 1.0 ppm (6-12 ppm)
Standard Solutions Method
(a)
Turbidity Nephelometric(LaMotte 2020)
Nephelometric Turbidity Units (NTU) 0.00 to 1100
NTU
1 NTU for readings below 100 NTU. ±3% above 100 NTU
1 NTU for readings below 100 NTU. ±3% above 100 NTU
Standard Solutions (NTU)
180.1
E. Coli and Total Coliform
Coliscan Easygel
# of fecal coliform (E.coli) & other coliform colonies
1 CFU/100mL 1 CFU/100mL N/A Micrology Laboratories, LLC 1996.
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Laboratory Parameter
Method/Range Units Detection Limit Precision CalibrationMethod
EPA Method Reference
Dissolved metals (Al, Sb, As, Ba, Be, Ca, Cd, Co, Cr, Cu, Pb, Fe, K, Mg, Mn, Mo, Na, Ni, P, Se, Th, Sn, Ti, V, Si, Ag, Zn)
ICP-MS scan µg/L See Appendix D RPD Limit = 10 Laboratory calibration: blanks & standards; initial calibration verification / quality control sample
200.8
Benthic macro-invertebrate bioassessment
Biological Assessment-- Technical Level. Samples collected in 1L Nalgene containers and preserved with 90% ethanol.
NA NA NA (b)
Method References:
(a) Indigo Carmine--ASTM D 888-87, Test Method A, Gilbert, T.W., Behymer, T.D., Castaneda, H.B., "Determination of Dissolved Oxygen in Natural and Wastewaters," American Laboratory, March 1982, pp. 119-134, and Rhodazine D method--(Method developed by CHEMetrics, Inc.) Power Plant Manual, First ed., p. 169 (1984)
(b) Macroinvertebrate Technical Level Biomonitoring Methods (AK SOP 001). April 1999. (UAA-ENRI)
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DATA GENERATION AND ACQUISITION B10. Sampling Process Design Sample Site Selection The Levelock Village Council selected three sample sites by reviewing topographic maps and discussing the importance of sample locations with respect to subsistence uses, potential impacts from development, and current use. Next, sample sites were visited by boat in July 2006 to verify sites, select a suitable stream reach, and to obtain GPS coordinates (Table T3): Table T3: Sample Sites and GPS Coordinates
Site Number
Site Description Latitude Longitude Notes
Site 1 Kvichak River, Below Egg Island
59º 15.055 N 156º 21.229 W Site ~200 yards below Egg Island.
Site 2 Yellow Creek 59º 10.813 N 156º 41.383 W Site is approximately 3 miles up Yellow Creek.
Site 3 Alagnak (Branch) River Above Old Village
59º 01.965 N 156º 39.207 W Site ~300 yards upriver from Old Village b/c gravel bar is present at Old Village.
Our sample sites are distributed throughout our watershed in areas that have seen little impact from development thus far. Our sites are safe and accessible in summer and winter when we will sample. Appendix E contains a topographic map marked with our sample sites. Sample sites will be located during sampling events using a hand-held GPS unit (minimum accuracy 20 feet). Sampling Parameters and Collection Frequency Sampling parameters have been selected based on their usefulness in determining the general "health" of our watershed. We will sample monthly in summer (June, July, and August) for a total of three sampling events per year. The following field parameters will be measured: air temperature, water temperature, dissolved oxygen, pH, conductivity, and turbidity. In addition, surface water samples will be sent to a laboratory samples for analysis of heavy metals. Benthic macroinvertebrate samples will be collected at the volunteer level twice per year at site 2. The water quality results will be compared to Alaska DEC and EPA water quality standards.
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Site Safety Plans The project coordinator and water quality technicians will exercise caution when monitoring and remember that safety comes first. Weather can be unpredictable throughout Alaska. Sampling will be stopped immediately if the field party feels the situation is unsafe due to weather, wildlife (bear) activity, or any other factor. The sampling team will wear shoulder-length rubber gloves when collecting samples at elbow-depth. Rubber boots or chest waders may be worn while sampling, as well as warm, waterproof clothing and insect protection. B11. Sampling Methods A tide prediction chart will be consulted prior to sampling so that it can correspond with the mid-day low tide (http://alaskaoutdoorjournal.com/References/Tides/bristoltides.html). Sampling will take place within the first seven days of the month on a Tuesday, Thursday, or Sunday to allow samples to be shipped on a scheduled Monday, Wednesday, or Friday PenAir flight to the laboratory. The sampling team will depart Levelock two hours before the mid-day low tide, boat to Site 1 (Kvichak River Below Egg Island), then sample Sites 2 (Yellow Creek) and 3 (Branch River Above Old Village) and return to Levelock. We estimate that each site will take one and a half hours to complete (except when benthic macroinvertebrate samples are collected). Thus, including transit time, the sampling will take approximately eight hours. Laboratory samples will be kept chilled and packaged to remain so until they reach the laboratory. Sampling methods are described in detail in Appendix C. Water quality parameters will be measured and lab samples collected at the point of highest flow of the stream (likely the mid-point). Field parameters and lab samples will be collected either from the upstream-side of a boat, or the upstream-side of sampling personnel standing in the stream wearing waders. Field measurements will be made in duplicate at the surface of the water with 10% distilled water field blanks. Lab samples will be collected at elbow-depth. All field equipment will be rinsed with distilled water between sample locations and stored in the proper storage solution at the end of each sampling day. Macroinvertebrate collection will occur after water quality measurements are made according to the “Standard Operating Procedures for the Alaska Stream Condition Index: A Modification of the U.S. EPA Rapid Bioassessment Protocols” guide that was produced by UAA-ENRI. The University of Alaska-Anchorage ENRI will be the support facility for bioassessment components of this project. The project coordinator is responsible for corrective actions for any improperly working equipment when sampling. Used specific conductance and pH calibration solutions will be combined in a leak-proof container and properly labeled. Spent CHEMetrics dissolved oxygen ampoules will be placed in a separate leak-proof container and also properly labeled. Waste storage containers will be stored in the Levelock Natives Limited building equipment cabinet until they are transported to Anchorage for disposal.
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B12. Sample Handling and Custody Laboratory samples will be collected in the field, labeled with sampling information, and immediately placed on ice/gel packs. Information from the sample label will be transferred to the data sheet for our records (Appendix F). Samples will be cared for (refrigerated or fresh gel packs added insure samples remain at 4 ºC) by the project coordinator and/or water quality technicians until they are released to the PenAir agent using the sample custody form provided by the laboratory. The samples will be sent to the laboratory who will record the date and time the samples arrive the lab and process samples according to standard operating procedures. Table T4: Sample parameter, method, container size, preservatives and hold time
Parameter Method Recommended
Container Preservative Hold Time
Metals, Dissolved (other than Hg or Hex.Cr) EPA 200.8
1x250 or 500 ml HDPE
Field = 4ºC, unpreserved Lab = filtered (0.45 µM) and then preserved with HNO3 180 days
Macroinvertebrate samples will be collected, labeled and preserved with ethanol as specified by ENRI. Field data sheets, sample bottles, and labels will be obtained from ENRI prior to sampling. The bottle label will include the site identification code, stream name, date and time of sample collection, number of each jar out of the total, the sampler’s name, and the preservation method (submersion in ethanol). This information will be recorded onto the field data sheet and kept in the project coordinator’s records. Samples will be kept in the possession of the project coordinator until they are released to the PenAir agent using a sample custody form, as detailed above for laboratory analysis. B13. Analytical Methods Documentation of methods used, along with precision and accuracy information, is provided in Table T2. Appendix C describes the analytical methods and equipment used in detail. All methods are EPA-approved and/or are used by numerous volunteer monitoring programs (e.g., Cook Inlet Keeper, Anchorage Waterways Council, etc.) and follow manufacturer instructions. The project coordinator is responsible for corrective actions for any improperly working equipment when sampling. Table T2 also identifies laboratory parameters that we will sample, as well as method detection limits for the lab samples. The dissolved metals samples will be filtered (0.45 µM) when they are received at SGS and subsequently preserved with HN03 until analyzed. The project officer is responsible for corrective actions for field measurements and for laboratory samples until they are released to SGS. Once in SGS’s custody, Heather Hall, SGS’s Quality Assurance officer
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will be responsible for any corrective actions. We have requested the standard turnaround time of 10 working days for this project. B14. Quality Control Prior to each sampling event, equipment will be calibrated and/or inspected. Replicate analyses will be performed for all field measurements, and 10% distilled water field blanks will be measured. Data that do not meet project accuracy and precision standards will not be entered into the Microsoft Excel spreadsheet. At the water quality training and annual recertification, the project coordinator and water quality technicians will be tested against known standards for field parameters. Techniques for all other parameters will also be reviewed. B15. Instrument/Equipment Testing, Inspection and Maintenance All equipment will be checked upon receipt by the project coordinator and/or water quality technicians to ensure that operations are within technical specifications before use. The expiration date of each calibration solution and test kit reagent will be noted prior to use. Before each sampling event, the project coordinator and water quality technicians will inspect all equipment to insure that it is clean and in good working order before being used for data collection. Spare parts will be brought to the field including extra turbidity tubes and calibration solutions. Equipment will also be evaluated at annual recertification sessions. Calibration solutions and reagents will be replaced prior to their expiration dates. Spare parts (turbidity tubes, calibration solutions, etc.) will be stored at the project coordinator’s office. An instrument calibration and maintenance log will be updated each time equipment is calibrated or maintained. This log will be kept on file in the project coordinator’s office. B16. Instrument/Equipment Calibration and Frequency The project coordinator and water quality technicians calibrate the Hanna Combo meter and turbidimeter as detailed in Appendix B. Equipment will be calibrated daily on sampling days, and if any data are questionable, the equipment will be recalibrated in the field. Calibration results will be recorded in the instrument calibration and maintenance log. Equipment will also be checked by the project QA officer at annual recertification session. All commercial laboratory instrumentation and equipment used in the analysis for this project will be calibrated prior to sample analysis according to the technical specifications and procedures specified in each analytical method used.
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B17. Inspection/Acceptance of Supplies and Consumables It is the responsibility of the project coordinator to ensure that all supplies and consumables are of adequate supply and quality to meet program needs. Supplies will be thoroughly checked upon arrival for any damage, and expiration dates of any calibration solutions will be recorded. Supplies and equipment are purchased under the supervision of the Levelock Village Council members, IGAP project officer, and any granting agencies or foundations that provide funding for this project. Equipment purchase receipts will be kept with the project coordinator records. B18. Data Acquisition Required longitude and latitude information for sample sites is derived from GPS data collected using a handheld unit and from USGS 1:63,360 topographic maps. Data gathered from sources other than selected site locations are used for information only and not for data entry or decision-making. Therefore, quality assurance of data acquired from other sources is not considered in this QAPP. B19. Data Management All field measurements will be recorded at the time of sampling on field data sheets. Data from the sheets will be entered into a Microsoft Excel spreadsheet by the project coordinator where accuracy/precision checks will be made. Data will be checked annually by the project QA officer to insure that they meet data quality objectives.
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ASSESSMENTS AND OVERSIGHT C20. Assessments and Response Actions The project coordinator and water quality technicians will be trained in water quality data collection and then recertified annually. After each sampling event, the project coordinator will review all data sheets before data are accepted and entered into the Microsoft Excel spreadsheet. Procedures for inspection, acceptance, calibration and maintenance of equipment and supplies are in place and described in previous sections and/or in Appendices B and C. The project QA officer will review the datasheets, Excel spreadsheet, and calibration logs on a semi-annual basis to confirm that data meet data quality objectives. Data that do not meet data quality objectives will be evaluated to determine the cause of the deficiency, (equipment malfunction, operator error, unrealistic DQOs, etc.) and a solution to the problem will be determined. Data that have been validated and appear not to meet state or national water quality standards will be flagged, and ADEC and EPA will be notified. C21. Reports to Management Reports satisfying IGAP workplan requirements will be written by the project coordinator to summarize the sampling activities at regular intervals. These reports will describe sampling activities, provide sample data in a table, note any data that do not meet ADEC or EPA water quality standards, and outline any changes that will be made to the QAPP to improve the sampling design. Summary reports will be made available to the Levelock Village Council members and sent to the IGAP Project Officer and all persons listed as technical resources in section A3. DATA VALIDATION AND USABILITY D22. Data Review, Validation and Verification Data will be reviewed by the project QA officer to confirm that the data meet QAPP objectives. Decisions to reject or qualify data will be made by the project coordinator and/or project QA officer. D23. Validation and Verification Methods All data from field data sheets will be entered into a Microsoft Excel spreadsheet by the project coordinator and reviewed. Data will be evaluated to insure they are within accuracy/precision limits in the spreadsheet. Verification of the data, decimal point, formulae, and completeness checks, will be performed by the Project QA officer on an annual basis.
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Equipment calibration and maintenance logs will be recorded and checked for deviations from typical calibration readings prior to sampling. Logs will also be entered into Microsoft Excel spreadsheets to back-up the information. D24. Reconciliation with Data Quality Objectives If data do not meet data quality objectives, the data will not be entered into the Excel spreadsheet. The cause of the problem will be determined. If the cause is found to be equipment failure, calibration and maintenance procedures will be reassessed and improved. If the problem is found to be the project coordinator’s protocols, she will seek guidance from the instructor. If accuracy and precision goals are frequently not being met, QC sessions will be scheduled more often. If failure to meet program specifications is found to be unrelated to equipment, methods or project coordinator/water quality technician error, specifications may be revised. Any revisions to the QAPP will be submitted to the ADEC and EPA QA Officers for approval. REFERENCES Alaska Division of Commerce Advocacy (ADCA), 2006. Alaska Community Database Information Summary for Levelock, Alaska. http://www.commerce.state.ak.us/dca/commdb/CIS.cfm Collins, C.N., and J.E. Dye, 2003. Survey of the Chinook and Coho Salmon Sport Fishery in the Alagnak River, Alaska, 2001 and 2002. Alaska Department of Fish and Game Division of Subsistence. Fisheries Data Series 03-12. Final report for study 01-173. Curran, J.H., 2003. Channel Stability and Water Quality of the Alagnak River, Southwest Alaska. USGS Water Resources Investigations Report 02-4184. Duffy, L.K. and X. Zhang, 2001. US Geological Survey Annual Report for project, “Mercury Levels in Alaska Rivers: Relationship Between Hg Levels and Salmon.” University of Alaska-Fairbanks. http://water.usgs.gov/wrri/AnnualReports/2001/FY2001_AK_Annual_Report.pdf Estes, C., 1998. Annual Summary of Instream Flow Reservations and Applications in Alaska. Alaska Department of Fish and Game Division of Sport Fish. Fisheries Data Series 98-40. Fair, L.F., 2003. Critical Elements of Kvichak River Sockeye Salmon Management. Alaska Fishery Research Bulletin. Vol. 10, No. 2, Winter 2003. Morris, J.M., 1985. The Use of Fish and Wildlife Resources by Residents of the Bristol Bay Borough, Alaska. Alaska Department of Fish and Game Division of Subsistence. Technical Paper Number 123.
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National Park Service (NPS) Water Resources Division, 1997. Baseline water quality, Data inventory and analysis: Lake Clark National Park and Preserve. NPS/NRWRD/NRTR-97/102. Fort Collins, CO. Sanders, L., 1998. A Manual of Field Hydrogeology. Prentice Hall: Upper Saddle River, 381 pp. USGS, 1984. Discharge Measurements at Gaging Stations, Techniques of Water-Resources Investigations of the USGS, Chapter A8. USGS, 2006. USGS surface water data for Alaska website: http://nwis.waterdata.usgs.gov/ak/nwis/sw UAA-ENRI, 1999. Macroinvertebrate Technical Level Biomonitoring Methods (AK SOP 001). Weeks, D.P., 1999. Katmai National Park and Preserve Water Resources Scoping Report. NPS Technical Report NRTR-99/226, 61 pp.
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Appendix A: Future Sampling Sites and Parameter Method Additions The Levelock Village Council hopes to add additional sampling sites and parameters once our sampling program is established. We know that we will gain a lot of insight in our first year of sampling, so we wish to increase the depth of our project after the first year, contingent on funding. These sites and parameters were recommended at the April 2006 community meeting in Levelock. Sites to be added Site Number Site Description Latitude Longitude Notes
Site 4 Kvichak River, Above Levelock 59º 06.976 N 156º 50.757 W Site ~500 yards upriver from Levelock boat landing. Site 5 Ben Courtney Creek 59º 15.551 N 156º 22.292 W Many aquatic plants at mouth of creek (Potamogeton spp.) Site 6 Alagnak (Branch) River Above
Jones’ 59º 04.576 N 156º 29.121 W Site ~300 yards upriver from Jones’.
Site 7 Kvichak River, Below Levelock 59º 06.181 N 156º 51.735 W Site ~200 yards below an anchored boat near George Wilson’s shop. Field Parameters If so desired, a YSI 556 multi-probe instrument may be purchased or rented for this project. Training to calibrate the probe would be required prior to its use. This probe would change the methods of the following parameters as detailed below: Parameter Method/Range Units Precision Accuracy Calibration
Method Method Reference
Temperature, water
YSI 556 -5.0 + 45.0 °C
Degrees Celsius (°C)
± 0.3 °C ± 0.15 °C NIST Certified Thermometer (-8 to 32°C)
EPA 170.1, SM 2550 B
pH YSI 556PH 0 to 14
Standard pH units (at 20°C)
± 0.3 units ± 0.2 units Standard Solutions (pH 7 and 4 or 10)
EPA 150.1, SM 4500-H+
Specific Conductance
YSI 556 0 to 100 mS/cm
µS/cm
± 0.1 mS/cm ± 0.5% of reading or ± 0.001 mS/cm, whichever is greater (4 m cable)
Standard Solutions (1413 µS/cm)
EPA 0120.1, SM 2510 B
Dissolved Oxygen
YSI 556 0 to 50 mg/L
mg/L
± 0.3 mg/L 0 to 20 mg/L, ± 2% of the reading or 0.2 mg/L, whichever is greater; 20 to 50 mg/L, ± 6% of the reading
Saturated Air Calibration
EPA 0360.1, SM 4500-O G
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Stream flow Global Flow Probe (FP-101) 0.3 to 25 (fps)
Feet per second (fps)
NA 0.1 fps Computercalibration and mechanical friction calibration of propeller bushing (factory)
003.4
Laboratory Parameters Parameter Method/Range Units Precision Accuracy Calibration Method Method Reference Total phosphorus Colorimetric mg/L MD = 0.0031 mg/L
PQ = 0.1 mg/L RPD Limit = 25 365.2
Total Nitrate-nitrogen Colorimetric mg/L MD = 0.031 mg/L PQ = 0.1 mg/L
RPD Limit = 25 353.2
Total Alkalinity Titration mg/L CaCO3 MD = 3.1 mg/L PQ = 10 mg/L
RPD Limit = 20
Laboratory calibration: blanks &
standards; initial calibration verification
/ quality control sample
SM 2320 B
Total Hardness Calculation mg/L CaCO3 Calculation Calculation N/A Calculation Sample parameters, methods, container size, preservatives and hold times
Parameter Method Recommended Container Preservative Hold Time Total Nitrate-nitrogen EPA 353.2 1x60ml Nalgene 4ºC 48 hours Total Phosphorus EPA 365.2 1x250 mL HDPE H2SO4; 4ºC 28 daysTotal Alkalinity as CaCO3 SM 2320B 1x250 or 500 ml HDPE 4ºC 48 hours Total Hardness Calculation N/a N/a N/a
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Appendix B: Instrument Calibration/Inspection Procedures and Recordkeeping The guide below outlines the procedures for instrument calibration and equipment inspection, which will be performed at the beginning of each day that surface water sample data collection takes place. Results from these checks will be recorded in the instrument calibration log. In addition, the dissolved oxygen test kit, flow meter, and Coliscan Easygel consumables will be checked to ensure good condition. If macroinvertebrates are to be collected, sample jars, ethanol, and sampling equipment will also be inspected.
Hanna Combo Meter (HI98129) pH
1. Meter should be stored in pH 7.01 solution. 2. Rinse clean, labeled beakers with small amounts of 4.01, 7.01 and 1413 µS/cm
calibration solutions and fill to 20 mL line. 3. Rinse meter with distilled water and place in beaker of 7.01 solution. 4. Turn meter on by pressing the MODE button. If meter is not in pH mode, press
SET/HOLD button until it is. 5. Allow meter to stabilize for two minutes in the 7.01 solution. 6. Record the pre-calibration pH and temperature reading in the calibration log 7. Press MODE button through the “off” reading until “cal” is shown on display, then release
button. 8. Display will read “7.01 use,” and “cal” will flash in the lower left-hand corner 9. Swirl meter and solution while instrument is calibrating 10. When display reads “4.01 use,” rinse probe with distilled water (over waste container) 11. Place probe in 4.01 calibration solution and swirl until “cal” stops flashing and probe goes
to measurement mode 12. Immediately record the post-calibration pH and temperature reading in the calibration log
Conductivity
1. Rinse probe again with distilled water and place in conductivity solution 2. Press SET/HOLD button once to reach conductivity mode 3. When reading is stable, record pre-calibration conductivity and temperature in calibration
log 4. Press MODE button through the “off” reading until “cal” is shown on display, then release
button. 5. Display will read “1413 use” and “cal” will flash in the lower left-hand corner 6. Swirl meter and solution while instrument is calibrating 7. When “cal” stops flashing, probe goes into measurement mode 8. Record the post-calibration conductivity and temperature readings in calibration log 9. Rinse probe with distilled water 10. Fill the small cylinder in the probe cap with the used pH 7.01 solution for storage. 11. Wash beakers with tap water and rinse with distilled water for storage.
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Turbidity Meter (LaMotte 2020) The utmost care will be taken to ensure cleanliness and prevent scratching of the turbidity tubes. A one-point calibration will be performed with either the 1.0 or 10.0 NTU standard, which will be selected according to the expected sample reading. If streams of varying turbidity are sampled, the LaMotte 2020 turbidimeter will be recalibrated accordingly between streams. Vials containing standards will be refilled with calibration solution if instrument readings/calibration are suspect. The operating mode will be set to EPA-mode for proper rounding of turbidity measurements.
1. Inspect turbidimeter, turbidity standards, and sample turbidity tubes for any damage 2. Ensure that all equipment and standards are at room temperature (or the same
temperature) to avoid condensation on the tubes. 3. Select the standard nearest the expected stream turbidity values, either 1.0 or 10.0 NTU 4. Clean selected standard using a Kimwipe 5. Align mark on turbidity tube with alignment mark in turbidimeter and insert tube 6. Close lid and push “read” button 7. If reading is the same as the standard value, turbidimeter is calibrated 8. If reading is different from the standard value, push “cal” button for 5 seconds until “cal” is
displayed 9. Display will flash. Use up or down arrows to adjust reading to the standard value. 10. Push “cal” button again. 11. Display stops flashing and calibration is complete 12. Turn unit off by pressing the “read” button for at least a second
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Hanna Combo Meter (HI 98129) Calibration Log pH 4 expiration date: pH 7 expiration date: Conductivity expiration date: Date Location Pre-
calibration pH 7
Post-calibration pH 4
Pre-calibration conductivity
Post-calibration conductivity
Comments
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Turbidity Meter (LaMotte 2020) Date standards received: Standard expiration dates (or 1 yr. from opening): Date Location Standard selected
(1 or 10 NTU) Initial standard reading Reading after
calibration
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CHEMetrics Dissolved Oxygen Vacuvials and Single Analyte Photometer Date Received: Expiration Date: Date Location Vacuvials condition Photometer Calibration Comments
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Coliscan Easygel Supplies Inspection Date received: Expiration date (1 yr. from receipt): Date Location Vials frozen? Petri Dish plates
OK? Comments
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Appendix C: Sample Analysis and Collection Procedures Upon arrival to the field sites, observations regarding the sample site will be recorded. A sketch of the sample location will also be made and digital photos taken of the sample site. The stream, river, or lake will subsequently be sampled by sampling the following parameters at elbow-depth. If the difference between two replicates exceeds the range of precision, a third measurement will be made immediately. Nitrile gloves will be worn during collection of all parameters. Dissolved Oxygen using CHEMetrics Kit (K-7512)
1. Fill the sample cup to the 25 mL mark with sample water collected at elbow depth. 2. Ensure that ampoules and comparator always remain in shade. 3. Place the CHEMet ampoule in the sample cup. Snap the tip by pressing the ampoule
against the side of the cup. 4. Mix the contents of the ampoule by inverting it several times, allowing the bubble to travel
from end to end each time. Wipe all liquid from exterior of ampoule. 5. Place ampoule in CHEMetrics dissolved oxygen single analyte meter compartment. 6. Read result.
Temperature, pH, and Specific Conductance
1. Turn meter on by pressing the “mode” button 2. Press “set/hold” button until probe is in pH mode 3. Allow probe to stabilize for approximately one minute 4. Record temperature reading (replicate 1). Wait 15 seconds, then record temperature
again (replicate 2) 5. Record pH reading (replicate 1). Wait 15 seconds, then record pH again (replicate 2) 6. Press “set/hold” button to change to conductivity mode 7. Record conductivity reading (replicate 1). Wait 15 seconds, then record conductivity
again (replicate 2) 8. Rinse probe with distilled water and fill cap reservoir with pH 7.01 solution for storage.
Turbidity
1. Ensure that turbidity tubes are clean. 2. Turn on turbidimeter 3. Rinse turbidity tubes with sample water three times 4. Holding necks of vials, place turbidity tube in sample water for approximately 1 minute.
This will allow the tubes’ temperatures to equilibrate to the water and will prevent condensation.
5. Fill and cap turbidity tubes under water to prevent air bubbles 6. Wipe turbidity tube thoroughly with a Kimwipe 7. Align indexing tube and meter arrows, insert tube into meter, and close lid 8. Insert tube 1 and press “read” button. 9. Repeat this action four additional times, recording each reading. 10. Turn meter off by pressing the “read” button for two seconds 11. Rinse turbidity tubes with distilled water prior to storage.
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Coliscan Easygel—Water Sample Collection 1. Label vial of Coliscan medium, put on clean nitrile gloves, and open sterile pipette 2. Collect water sample by inserting pipette directly into the water to elbow depth 3. Two samples should be collected per site, one sample of 1 mL of sample and one of 5
mL of sample 4. Place water samples directly into Coliscan medium vials 5. Store samples on ice in cooler until return to LVC.
Laboratory Sample Collection Procedures
1. Locate the highest point of flow of the sample site. 2. Dip sample bottle on the upstream side of sample collector (site 2) or boat (sites 1& 3) to
elbow-depth. 3. Fill sample bottle to shoulder of bottle and place in cooler. Samples will remain chilled
until arrival to the laboratory. Macroinvertebrate Collection and Preservation (Technical Level) Full method description and example data sheets can be found at http://aquatic.uaa.alaska.edu/BiolMonitoringResources.htm
1. Macroinvertebrates will be collected following the “Macroinvertebrate Technical Level Biomonitoring Methods (AK SOP 001)”. April 1999. (UAA-ENRI)
2. Select a 100-meter stream reach near the sample site that is suitable for bioassessment 3. Complete the “Physical Characterization” and “Benthos/Water Quality” sheets. 4. Ensure that nets are clean 5. Starting at the downstream part of the stream reach, collect samples with twenty
kicks/jabs 6. Place all sediment in a 1-L Nalgene bottle (2-3 bottles may be needed) 7. Carefully drain any excess water from the bottles 8. Preserve the sediment with 95% ethanol so that samples are completely covered in
ethanol 9. Label sample containers with the information listed on the field data sheets 10. Keep samples cool and out of sunlight while transporting for ENRI 11. ENRI will analyze samples and provide the LVC with results.
Coliscan Easygel—Plating and Enumerating
1. Upon return to Levelock, prepare a work area for Coliscan Easygel sample processing. Samples should be plated within six hours of sample collection.
2. Sterilely transfer contents of each Coliscan medium vial (with sample water) to a correctly-labeled Petri dish
3. Cover Petri dish, and gently swirl contents to cover bottom of dish 4. Allow dish to become firm for approximately 40 minutes. 5. Invert Petri dishes, place in large Ziplock bag, and place in incubator 6. Incubate at 35 ºC (95 ºF)for 24 hours 7. After 24 hours, count bacteria. Categorize to blue/purple (E. Coli), pink (other coliforms),
and teal (non-coliform) bacteria.
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8. Upon completion of counting, place one teaspoon of bleach on the medium of each dish and allow to sit for five minutes
9. Place in leak-proof bag and discard in trash. 10. Calculate number of colonies per 100 mL of water using the following formula:
(100 mL / sample volume) x number of colonies = number of colonies / 100 mL
So, for a 2 mL water sample that produced 6 coliform colonies:
100 mL / 2 mL = 50 x 6 coliform colonies = 300 coliform colonies / 100 mL
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Appendix D: ICP-MS Scan (EPA 200.8) Detection and Reporting Limits
Analyte Method Detection Limit (MD) (µg/L) Reporting Limit (PQ) (µg/L)
Aluminum 6.2 20 Antimony 0.31 1 Arsenic 1.5 5 Barium 0.94 3
Beryllium 0.13 0.4 Calcium 150 500
Cadmium 0.05 0.5 Cobalt 1.2 4
Chromium 0.31 1 Copper 0.31 1 Lead 0.062 0.2 Iron 78 250
Potassium 150 500 Magnesium 15 50 Manganese 0.31 1 Molybdenum 3.1 10
Sodium 150 500 Nickel 0.62 2
Phosphorus 62 200 Selenium 1.5 5 Thallium 0.31 1
Tin 0.31 1 Titanium 1.5 5
Vanadium 6.2 20 Silicon 62 200 Silver 0.31 1 Zinc 1.5 5
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Appendix E: Map of Sampling Sites
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Appendix F: Water Quality Data Collection Field Sheet
Date: Site name: Time arrived at site:
Latitude (GPS): Waypoint name (GPS): Longitude (GPS): GPS Accuracy (min. 20 ft.): Site description: Data collectors:
Air temperature (°C, shade): Weather condition (circle one):
Cloudless. Thunderstorms. Squalls. Rain, sleet, snow, or hail. Rain showers. Drifting snow, or dust/sand storm. Visibility less than 1000 M Fog or dust. Visibility less than 1000 M. Drizzle or light rain. Snow, sleet, or hail. Rain. Overcast. Cloudy or partly cloudy. Other ___________________________________
Water Physical Appearance (circle one): Clear - crystal clear, transparent water
Green OR Muddy plus extensive floating scum/foul odor Green - algal coloration evident Foamy - natural or from pollution Tea-colored - clear, natural coloration from wetland Muddy - cloudy brown due to high sediment levels Milky - not quite crystal clear; cloudy white or gray Other ___________________________________
Wildlife Observed:
Comments:
Photographs taken at site?
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Site Sketch (mark sample site):
Dissolved oxygen (CHEMetrics DO reading on SAM) Start time: Replicate 1 Replicate 2 Replicate 3 Dissolved Oxygen (ppm) Water Temperature/pH/Specific Conductance (Hanna Combo Meter) Start time: Replicate 1 Replicate 2 Replicate 3 DI Blank Water Temperature °C pH Specific conductance (µS/cm)
Turbidity (LaMotte 2020) Start time: Measurement
1 Measurement
2 Measurement
3 Measurement
4 DI Blank
Turbidity (NTU) Turbidity (NTU) Lab Samples Start time: Time Bottle Filled Time Bottle Filled
(Replicate) Metals Coliscan Easygel Start time: Time Bottle Filled Time Bottle Filled
(Replicate) Time Bottle Filled
(DI blank) Coliscan Easygel Macroinvertebrates Samples collected? Yes _______ No ________