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QoD (HB) 5-31-11QoD (HB) 5-31-11
Do you remember what a plasmid Do you remember what a plasmid is?is?
Plan of the Day (HB)Plan of the Day (HB)How engineering bacteria works Overview and points to consider for
pGLO bacterial transformation lab tomorrow
PresentationsPresentations
How can bacteria exchange genes?
Through tunnel like connectionsViruses carry bacterial genesBacteria take up DNA from
surrounding environment
Recombinant DNA Technology
• Some Bacteria are used to mass produce specific desirable genes and proteins
• Restriction enzymes cut DNA molecules at specific locations
• Fragments of DNA spliced together from two different sources produces a recombinant DNA molecule
Applications of recombinant DNA technology
• Bacteria engineered to clean up toxic waste• Mass-production of useful chemicals• Mass production of proteins and hormones for
medical uses• Develop vaccines against disease-causing
microbes• Develop genetically modified multicellular
organisms
pGLO bacterial transformation lab preparation
http://biologywithsaxe.blogspot.com/2009/01/lab-9-bacterial-transformation-using.html
Allows bacteria to survive in the presence of ampicillin
In the presence of arabinose produces a protein that will turn on the GFP gene
When turned on will produce a fluorescent green protein that will glow under UV light