QMAS - Quality in Meat and Fish Analysis PT Scheme Round: 233siteform.tonynetcn.com/X_UpDown/10000852-D1015664ECBD2A21/... · [1] ISO 13528 (2005), ‘Statistical methods for use

QMAS - Quality in Meat and Fish Analysis PTScheme

Round: 233

Issue Number 1

Issued 23 October 2015

LGC | 1 Chamberhall Business Park | Chamberhall Green | Bury | Lancashire | BL9 0AP | UK |+44(0)161 762 2500 | [email protected] | www.lgcstandards.com

Aims of SchemeThe primary aim of the Quality in Meat Analysis Scheme (QMAS) is to enable laboratories performing the analysis of meat and fish products to monitor their performance and compare it with that of their peers. QMAS also aims to provide information to participants on technical issues and methodologies relating to the chemical and microbiological examination of meat.

Further information on the scheme organisation, the test materials, and the statistical analysis of data are available in the QMAS Scheme Description and the LGC PT General Protocol.

Performance AssessmentOnce a PT round has closed, the results will be analysed and the assigned value determined for each analyte, according to the criteria provided in the Scheme Description. Information regarding the traceability of each calculated assigned value is also provided in the Scheme Description.

For quantitative data, the participant’s result, x, (or log10 x for microbiological data) is converted into a z score using the following formula;

z = (x - X) SDPA

X = Assigned valueSDPA = Standard deviation for proficiency assessment

For quantitative data, the uncertainty of the assigned value is calculated to ensure that it would have a negligible effect on participants’ performance scores. If the uncertainty of the assigned value is greater than 0.3 x SDPA, then this is not considered negligible. In this situation, a z’ (z prime) performance score is automatically calculated rather than a z score, in order to take account of the measurement uncertainty of the assigned value. The z’ score is calculated using the following formula;

z’ = (x - X) √(SDPA2 + UxAV2)

X = Assigned value SDPA = Standard deviation for proficiency assessmentUxAV= Uncertainty of the assigned value ____ Expanded SDPA = √(SDPA2 + UxAV2)

Trend graphs will use a mixture of z and z’ scores, i.e. the ‘performance score’ for the round.

For quantitative data, gross errors or blunders are removed from the data by removal of any results that are greater than the assigned value ± 5 x SDPA. These results are not used in the final calculation of the assigned value and other summary statistics and will be included in the number of ‘Excluded Results’. All results, including excluded results, will be given a performance score.

For the purposes of performance assessment for a single round, z and z’ scores are interpreted as follows:

z/z’ score Interpretation Colour codingçzç≤ 2.00 Satisfactory result Green2.00 < çzçand < 3.00 Questionable result Amberçzç≥ 3.00 Unsatisfactory result RedNo score given See below No colour coding

Performance scores will not be given for the following:

§ For qualitative results, where satisfactory performance is based on the participants reporting the same result as the assigned result. e.g. detected, not detected. For these results, colour coding of green (satisfactory) or red (unsatisfactory) will apply.

§ For results of zero; such a result is not normally appropriate and should not be reported, the result should be reported as less than the detection limit rather than zero.Note: for a very small number of analytes it may be appropriate to report a result of zero, depending on the type of measurement scale being used.

§ For quantitative results where the analyte under test is present in the test material but participants report non-numerical results e.g. 0, <1, >300. In these cases, it is not possible to allocate a performance score and participants should assess their performance based on the assigned value and satisfactory range given.

§ For quantitative results, for microbiological test materials, where the analyte under test is not present in the test material, the assigned value will be classified as ‘Absent’. Results reported as ‘less than’ at or below the detection level for our method of confirmation will be assessed as satisfactory (green colour code). Results reported at a higher detection level will not be assessed and participants will need to use their own judgement to determine whether their result is fit for its intended use. Results reporting a positive count will be assessed as unsatisfactory (red colour code).

In some cases, performance scores may not be provided or may be provided but with colour coding suspended (indicating that scores need to be interpreted with caution). For example:

§ For small data sets where less than 8 results have been submitted and the assigned value is derived using a consensus value from the participants’ results. In these circumstances, there may be increased uncertainty of the assigned value, given the low number of participants, and performance scores will be given for information only.

§ In cases where the distribution of the results gives cause for concern e.g. bi-modal data sets. These circumstances will be dependent on the statistical design that is in place.

Round: 233Scheme: QMAS - Quality in Meat and Fish Analysis PT Scheme

Scheme Information

Issue: 1 Page 2 of 69 23 October 2015


§ If the assigned value falls below a concentration threshold (only applies to some schemes).

In these or similar circumstances, further explanation as to the reasons for suspension of performance scoring or colour coding, and on the interpretation of results, will be given in the report.

Note: Data displayed in the report will have been rounded to the required number of decimal places. However statistical calculations will have been performed on unrounded data. For this reason, there may appear to be differences between displayed data and calculated data, but this does not affect results in any way.

ConfidentialityA unique laboratory reference code is used to report results in order to ensure confidentiality.

Contact detailsThe technical scheme coordinator is (Micro) Andrew Cheetham.The technical scheme coordinator is (Chem) Wayne Gaunt.

Please contact [email protected] if you have any questions or comments regarding the scheme.

Authorisation

This report was authorised by Karen Cliff, Microbiologist on the 23 October 2015



Samples were despatched on 21 September 2015The reporting deadline was 16 October 2015

The following samples were distributed in QMAS Round 233:

Sample Matrix Contents735 10g Lyophilised meat Escherichia coli

Klebsiella oxytoca

736 25g Lyophilised meat Salmonella Panama

737 25g Lyophilised meat Listeria monocytogenes

738 10g Lyophilised meat Clostridium perfringensStaphylococcus aureus

739 Lyophilised vial with 10g Staphylococcus aureuslyophilised shellfish Escherichia coli

740 Lyophilised vial with 25g Salmonella Hadarlyophilised shellfish Escherichia coli

743 Lyophilised vial with 25g Campylobacter hyointestinalislyophilised meat

744 Lyophilised vial with 25g Escherichia coli O157 (Non-toxigenic strain)lyophilised meat

745 Lyophilised vial with 25g Vibrio parahaemolyticuslyophilised shellfish

746 Lyophilised vial with 10g Pseudomonas aeruginosalyophilised meat Pichia fermentans

Fusarium moniliforme

748 Foil bag with 150g fish

Further information regarding assigned values, performance assessment and technical comments can be found under the individual sample and analyte results.

Sample Details

All homogeneity assessments have been conducted in accordance with the principles stipulated in ISO 13528 [1] and the IUPAC [2] Harmonized PT Protocol. Further details regarding the assessment of homogeneity can be found in the LGC Standards Proficiency Testing General Protocol.

Sample Analyte/Test Method Result735 Total viable count PCA PP 30 Pass736 Salmonella species detection MKTT/RV + XLD + BGA 37 Pass737 Listeria species detection FRASER + ALOA 37 Pass

Listeria monocytogenes detection FRASER + ALOA 37 Pass738 Clostridium perfringens enumeration TSC PP 37 Pass739 Total viable count PCA PP 30 Pass740 Salmonella species detection MKTT/RV + XLD + BGA 37 Pass743 Campylobacter species detection BOLTON + CCDA/SKIR 42 Pass744 Escherichia coli O157 detection BROTH + CT-SMAC Pass

(non-toxigenic strain)745 Vibrio species detection APW + TCBS 37 Pass746 Total viable count PCA PP 30 Pass7481 TVN Titration Pass

1Analyses were performed, by Exova, in duplicate on randomly selected test materials.

For quantitative testing, one-way ANOVA is used to estimate the analytical and sampling variance. For the sample to be considered sufficiently homogeneous for use in the PT scheme, the calculated sampling variance must be less than a critical value calculated according to the procedure described in the IUPAC [2] Harmonized PT Protocol.

For qualitative testing, the target analyte must be detected in 100% of test materials analysed.

Often a particular test material does not require homogeneity assessment prior to distribution. Such sample types include standard solutions and aqueous solutions.

Test materials provided under the LGC scope of accreditation are assessed to ensure that they are sufficiently stable for the duration of the PT round. If test materials are subsequently shown to have changed significantly through the time of the PT round either, the effect of the instability will be quantified and taken into account in the performance evaluation or participant performance will not be evaluated.

Quality Control



[1] ISO 13528 (2005), ‘Statistical methods for use in proficiency testing by inter-laboratory comparisons’.

[2] M Thompson, S L R Ellison, R Wood, ‘International Harmonised Protocol for the Proficiency Testing of Analytical Chemistry Laboratories’, Pure Appl. Chem., 2006, 78, 145-196.


Sample: 735 - Meat Indicator Combination

Analyte: Total aerobic mesophilic count


z scoreResult (cfu/g)MethodLab ID

0.8116,600PetrifilmMT0003

-0.545,578PCA PP 30MT0013


-0.147,700PCA PP 30MT0029

0.2710,700PCA PP 30MT0036

0.5813,727OtherMT0052

0.1810,000PCA PP 30MT0068

-0.177,500PCA PP 30MT4040

-0.266,980PCA SP 30MT4065

0.6314,300PCA PP 30MT4086

0.059,000PCA SP 30MT4102

0.3011,000OtherMT4102

0.6915,000PCA PP 30MT4214

0.089,180PCA PP 30MT4261

0.3511,400PCA PP 30MT4263

0.2710,700PCA PP 30MT4266

-0.167,600OtherMT4280

-0.217,300PCA PP 30MT4305

-0.137,750PetrifilmMT4332

0.3611,500PCA SP 30MT4334

-0.028,500PCA PP 30MT4346

0.3011,000OtherMT4354

0.1810,000PCA PP 37MT4388

0.179,900OtherMT4397

0.3111,090PCA PP 30MT4398

-0.098,000PCA SP 30MT4417


-0.028,500PCA PP 30MT4463

0.089,200PCA PP 30MT4486

0.1810,000PCA SP 30MT4541


0.4212,100PCA SP 30MT4562

0.1810,000PCA PP 30MT4564

-0.088,100PCA SP 30MT4564

-0.336,600PCA PP 30MT4580

0.1810,000PCA PP 30MT4589

-0.655,100PCA PP 30MT4593

0.079,100PCA PP 30MT4639

-0.247,100PCA PP 30MT4645

-0.028,500PCA PP 30MT4664

-0.535,600PCA PP 30MT4739


-0.058,300TEMPOMT4779

1.1121,000OtherMT4800

0.169,800PCA PP 30MT4803

2.4160,000PCA PP 30MT4836

2.4160,000PCA SP 30MT4836


-0.137,750PCA PP 30MT4842


-0.495,800OtherMT4894

0.5012,909PCA PP 30MT4903

-0.098,000PCA PP 30MT4959

0.129,500PCA PP 37MT4964

0.5613,500PCA PP 30MT4988

-0.286,900PCA PP 30MT5026

-0.127,800TEMPOMT5026



-0.127,800TEMPOMT5097

0.6314,363PCA PP 30MT5138

-0.127,830PCA PP 37MT5138

Page 6 of 69Issue: 1 23 October 2015






-0.028,500PCA PP 37MT5157

0.099,300PCA PP 37MT5175

-0.814,500PCA PP 30MT5191

0.2610,640PCA PP 30MT5192

0.6814,909PCA PP 37MT5212

-0.237,167OtherMT5221

-0.336,600PCA PP 30MT5221


0.008,636PCA PP 30MT5321

0.2810,772PCA PP 37MT5321

0.1810,000PCA SP 30MT5337

-0.177,500PCA PP 30MT5342

0.129,500PCA PP 30MT5343

0.139,600PCA PP 30MT5345

-0.655,100PCA PP 30MT5384



0.018,700PCA PP 30MT5440


0.7716,000PCA PP 30MT5469


-0.167,600PCA PP 30MT5483

-0.247,100OtherMT5536

-0.127,800PCA PP 37MT5597


-0.207,364PCA PP 30MT5698

-0.495,800PCA PP 37MT5731


0.008,600PCA PP 30MT5780

0.4112,000PCA PP 30MT5781


-2.311,340PCA PP 37MT5839

0.2110,200PCA SP 30MT5839

2.0244,000PCA PP 30MT5863

-0.267,000OtherMT5915

-3.51510OtherMT5955

-4.67200OtherMT5971

0.4112,000PCA PP 30MT5985


-0.267,000PCA SP 30MT6022









-0.316,700OtherMT6047

0.2910,864PCA PP 30MT6051

-2.98780PCA PP 30MT6067



-0.535,610OtherMT6077

-0.296,800MPCA PP 30MT6105


5.09520,000PCA PP 30MT6126




0.1810,000OtherMT6156





Value

Data Statistics

124Number of Results

2Number of Excluded Results

3.92 log10Mean

3.94 log10Median

0.31 log10Standard Deviation

0.13 log10Robust Standard Deviation

200 to 60000 cfu/gResult Range

Performance Statistics

Value

8,618 cfu/gAssigned Value

0.02 log10Uncertainty of Assigned Value

0.35 log10SDPA

1,720 to 43,192 cfu/gSatisfactory Range

91.1%Satisfactory z scores

5.6%Questionable z scores

3.2%Unsatisfactory z scores





Methodology Summary

%Log10TotalResultsResultsMethod

Sat.RangeRobust SDMedian% ofExcludedNumber of

92.0780 to 600000.123.9640.32150PCA PP 30

90.96980 to60000

0.124.008.87011PCA SP 30

90.01340 to14909

0.083.958.06010PCA PP 37

100.06800 to 68000.003.830.8101MPCA PP 30

91.21000 to60000

0.143.9227.42134Petrifilm

100.07800 to 83000.013.892.4203TEMPO

86.7200 to 210000.213.8612.1015Other

91.1200 to 600000.133.941002124All

Comments

The sample contained Escherichia coli and Klebsiella oxytoca. The assigned value for total aerobic mesophilic count is 8,618 cfu/g.

Total aerobic mesophilic counts are used to determine the general microbial population of a sample. Bacterial swabs performed on meattypically contain 102 to 104 bacteria per cm2, counts greater than 105 per cm2 may indicate poor hygiene.



Analyte: Enterobacteriaceae




-0.604,275VRBGA PP 37MT0013


0.067,300VRBGA PP 37MT0029

-0.086,500VRBGA PP 37MT0036

-0.385,136VRBGA PP 37MT0052

-0.853,500VRBGA PP 37MT0068

0.017,000VRBGA PP 37MT4040

-0.624,200VRBGA PP 30MT4065

-0.126,300VRBGA PP 30MT4086

-0.036,800OtherMT4102

0.5711,000MPNMT4102

0.339,090VRBGA PP 37MT4261

-0.464,800VRBGA PP 37MT4263

0.318,900VRBGA PP 37MT4266

-0.275,600OtherMT4280

0.037,100VRBGA PP 37MT4305


0.439,840VRBGA PP 30MT4334

-0.315,400OtherMT4354

0.147,800VRBGA PP 37MT4388

0.017,000OtherMT4397

0.288,727VRBGA PP 37MT4398

-0.146,200VRBGA PP 30MT4417


-0.434,900VRBGA PP 37MT4463

0.097,500VRBGA PP 37MT4486

0.047,200VRBGA PP 30MT4541


0.218,200VRBGA PP 37MT4562

0.329,000VRBGA PP 37MT4564

-1.003,100VRBGA PP 37MT4580

-0.604,300VRBGA PP 30MT4589

-0.415,000VRBGA PP 30MT4593

0.137,700VRBGA PP 37MT4639

-0.295,500VRBGA PP 37MT4645

-0.385,100VRBGA PP 37MT4664

0.037,100VRBGA PP 37MT4739


-0.544,500TEMPOMT4779

-0.146,200VRBGA PP 30MT4803


2.2041,000VRBGA PP 37MT4836

-0.016,900VRBGA PP 37MT4842

-0.205,900OtherMT4894

0.5110,500VRBGA PP 37MT4903

-0.823,600VRBGA PP 30MT4959

0.318,900VRBGA PP 37MT4964

0.6812,000VRBGA PP 37MT4988

-0.434,900VRBGA PP 37MT5026

0.339,100TEMPOMT5026


-0.255,700TEMPOMT5097

1.0215,818VRBGA PP 37MT5138

-0.086,520OtherMT5138

<10PetrifilmMT5148


0.399,500VRBGA PP 37MT5191

0.097,490VRBGA PP 30MT5192

0.7512,727VRBGA PP 37MT5212

-0.046,718VRBGA PP 37MT5221







-0.793,682VRBGA PP 37MT5321

-0.624,227OtherMT5321

0.339,100OtherMT5328

0.137,700VRBGA PP 30MT5337

-0.036,800VRBGA PP 37MT5342

0.6812,000VRBGA PP 37MT5343

-0.205,900VRBGA PP 37MT5345

-0.783,700VRBGA PP 37MT5384


-0.753,800VRBGA PP 30MT5384


0.097,500VRBGA PP 37MT5440

0.7813,000VRBGA PP 37MT5469


0.147,800VRBGA PP 30MT5483

-0.345,300VRBGA PP 37MT5597


-0.255,700VRBGA PP 37MT5698

-0.036,800VRBGA PP 37MT5780

0.409,600VRBGA PP 37MT5781

0.7412,600VRBGA PP 37MT5839

-0.415,000OtherMT5915

-4.60170PetrifilmMT5971

0.6812,000VRBGA PP 37MT5985

0.017,000VRBGA PP 37MT6022









-0.494,700OtherMT6047

0.359,182VRBGA PP 37MT6051

-2.87690VRBGA PP 37MT6067





0.9515,000VRBGA PP 37MT6126



-1.981,411VRBGA PP 37MT6136

-0.255,700OtherMT6156

Value

Data Statistics



3.81 log10Mean

3.84 log10Median









Value



0.35 log10SDPA





Methodology Summary



95.9690 to 410000.193.8545.79049VRBGA PP 37

100.03600 to 98400.143.7912.15013VRBGA PP 30

89.7170 to 450000.163.8528.04130Petrifilm

100.011000 to11000

0.004.040.9301MPN

100.04500 to 91000.153.762.803TEMPO

100.04227 to 91000.093.7610.28011Other

95.3170 to 450000.173.841001107All

Comments

The sample contained the Enterobacteriaceae Escherichia coli and Klebsiella oxytoca at an assigned value of 6,950 cfu/g.

Members of the Enterobacteriaceae family are widely found in soil, water, fruits, vegetables, grains, flowering plants and a wide range of animals, from insects to humans.



Analyte: Coliforms



-0.036,600VRBA PP 37MT0029

0.409,300VRBA PP 37MT0036

0.439,545Chromogenic agarMT0052

-0.375,000VRBA PP 37MT0068

-0.893,300VRBA PP 37MT4040

-0.604,160VRBA PP 30MT4065

-0.404,900VRBA PP 37MT4086


0.258,270VRBA PP 37MT4261

-0.424,800VRBA PP 37MT4263


0.067,100VRBA PP 30MT4305

0.5510,500COLI ID PP 37MT4334

0.057,000VRBA PP 37MT4346

-0.215,700OtherMT4354

0.067,100VRBA PP 30MT4388


0.187,818VRBA PP 37MT4398


0.248,200VRBA PP 37MT4486




0.389,200VRBA PP 37MT4564

-0.325,200VRBA PP 37MT4580


-1.512,000Chromogenic agarMT4593

0.157,600VRBA PP 37MT4639

-0.285,400VRBA PP 37MT4645

-0.654,000VRBA PP 30MT4739


0.036,900OtherMT4779

0.9915,000VRBA PP 37MT4800

-0.056,500COLI ID PP 37MT4803

2.4649,000COLI ID PP 37MT4836

2.3545,000OtherMT4836


-2.371,000OtherMT4842


-0.195,800OtherMT4894

0.429,500OtherMT4903

0.298,500VRBA PP 37MT4964

0.7112,000VRBA PP 30MT4988

0.057,000COLI ID PP 37MT5003

-0.404,900VRBA PP 37MT5026

-0.156,000OtherMT5026



0.389,200OtherMT5097

0.6911,727VRBA PP 37MT5138


<10PetrifilmMT5148

0.087,200VRBA PP 37MT5157


0.137,500VRBA PP 37MT5191

0.6111,000MPNMT5192

0.228,091VRBA PP 37MT5212

-0.195,794VRBA PP 37MT5221


-0.444,727VRBA PP 37MT5321

-0.295,364OtherMT5321

0.409,300MPNMT5328



Analyte: Coliforms




-0.504,500VRBA PP 37MT5342

-0.056,500VRBA PP 37MT5343

-0.136,100VRBA PP 37MT5345

0.7112,000OtherMT5384




0.278,400VRBA PP 30MT5440


0.6111,000VRBA PP 37MT5469


0.207,900VRBA PP 37MT5483

-0.285,400OtherMT5536

-0.116,200OtherMT5597


-0.325,200VRBA PP 37MT5698

-0.484,600MPNMT5731


0.016,800VRBA PP 30MT5780

0.4910,000VRBA PP 37MT5781


0.4910,000VRBA PP 37MT5839

0.8113,000VRBA PP 30MT5863

0.4910,000OtherMT5915

-5.3690OtherMT5955

-10.082Chromogenic agarMT5971










-0.624,100OtherMT6047

-2.76732VRBA PP 30MT6067


-0.793,580OtherMT6077

-0.305,300VRBA PP 37MT6105


-2.251,100MPNMT6126




-0.136,100OtherMT6156

Value

Data Statistics



3.80 log10Mean

3.83 log10Median






Analyte: Coliforms



Value



0.35 log10SDPA





Methodology Summary



100.03300 to15000

0.183.8529.36032VRBA PP 37

88.9732 to 130000.343.858.2609VRBA PP 30

75.06500 to49000

0.153.933.6704COLI ID PP 37

75.01100 to11000

0.283.823.6704MPN

89.7500 to 450000.193.7327.52130Petrifilm

92.32000 to11000

0.113.9011.93113Chromogenicagar

82.41000 to45000

0.173.7815.6117Other

90.7500 to 490000.193.831003109All

Comments

The sample contained the coliforms Escherichia coli and Klebsiella oxytoca at an assigned value of 6,750 cfu/g.

Coliforms are found in animal intestines, and their presence in foods is used as an indicator of faecal contamination. However, manycoliforms are also found throughout the environment, in air, soil, water, plants and animals. The presence of coliform indicator organisms inmeat may, therefore, be indicative of faecal contamination, but may also be environmental in origin.



Analyte: Escherichia coli




-0.881,970TBX PP 37-44MT0013



0.425,600COLI ID PP 37-44MT0036


0.004,000TBX PP 37-44MT0068

-0.103,700TBX PP 37-44MT4040

-0.223,360TBX PP 37-44MT4065

0.034,100VRBA PP 37-44MT4086


0.355,300MPNMT4102

0.837,800OtherMT4102

0.285,000TBX PP 37-44MT4261

-0.133,600TBX PP 37-44MT4263


0.485,880OtherMT4305

0.686,920COLI ID PP 37-44MT4334

-0.532,600OtherMT4354


0.546,200OtherMT4397

0.285,000TBX PP 37-44MT4398



0.204,700TBX PP 37-44MT4486



0.204,700TBX PP 37-44MT4562

0.375,400TBX PP 37-44MT4564


0.375,400TBX PP 37-44MT4593

0.355,300TBX PP 37-44MT4639

0.405,500TBX PP 37-44MT4645

-0.802,100COLI ID PP 37-44MT4664

-0.402,900TBX PP 37-44MT4739


0.004,000OtherMT4779

1.4613,000VRBA PP 37-44MT4800

-0.103,700COLI ID PP 37-44MT4803

2.7336,000COLI ID PP 37-44MT4836


2.6935,000TBX PP 37-44MT4836

-0.103,700TBX PP 37-44MT4842

-0.492,700OtherMT4894

0.717,100OtherMT4903


-2.68460MPNMT4964


0.285,000COLI ID PP 37-44MT5003

0.405,500OtherMT5026



0.757,300OtherMT5097


-0.093,710TBX PP 37-44MT5138

<10PetrifilmMT5148



-0.033,900TBX PP 37-44MT5191

-0.063,800TBX PP 37-44MT5192

1.039,182COLI ID PP 37-44MT5212

-0.402,900OtherMT5221






-0.173,500TBX PP 37-44MT5221


-0.323,091TBX PP 37-44MT5321

0.164,545OtherMT5321

1.059,300MPNMT5328


0.405,500TBX PP 37-44MT5342

-0.103,700OtherMT5343

0.375,400TBX PP 37-44MT5343

0.174,600TBX PP 37-44MT5345


-0.442,800OtherMT5384



0.405,500TBX PP 37-44MT5440


-0.173,500TBX PP 37-44MT5469


-0.402,900TBX PP 37-44MT5483

-4.9375MPNMT5536



0.054,181TBX PP 37-44MT5698

-1.221,500MPNMT5731



0.606,500TBX PP 37-44MT5781


0.697,000TBX PP 37-44MT5839

0.898,200TBX PP 37-44MT5863

0.606,500OtherMT5915

-8.076OtherMT5955


0.405,500TBX PP 37-44MT5985

-0.173,500TBX PP 37-44MT6022









-0.632,400OtherMT6047

0.506,000TBX PP 37-44MT6051

-2.94375TBX PP 37-44MT6067




-0.632,400MPNMT6104


-2.68460MPNMT6126



0.485,900OtherMT6156





Value

Data Statistics



3.60 log10Mean

3.60 log10Median





Value



0.35 log10SDPA

798 to 20,047 cfu/gSatisfactory Range








Methodology Summary



94.3375 to 350000.143.6629.91035TBX PP 37-44

85.72100 to36000

0.273.755.9807COLI ID PP 37-44

100.04100 to13000

0.373.861.7102VRBA PP 37-44

94.3300 to 350000.183.5430.77136Petrifilm

57.175 to 93000.763.185.9807MPN

92.33300 to10636

0.143.6711.11113Chromogenicagar

94.12400 to 78000.213.7014.53117Other

91.475 to 360000.213.601003117All

Comments

The sample contained Escherichia coli at an assigned value of 4,000 cfu/g.

Escherichia coli is found in animal intestines, therefore the presence of the organism in meat indicates poor hygiene practice as the meatmay have become contaminated during the slaughtering process. It is also historically used as an 'indicator' organism, to indicate whethermore serious pathogens, such as Salmonella are also likely to be present. However, this organism is also widespread throughout theenvironment, being isolated from air, soils, water, plants and animals. Therefore the presence of Escherichia coli in meat may be faecal orenvironmental in origin.


Analyte: Salmonella species

Sample: 736 - Meat Salmonella (P/A)


ResultMethodLab ID

DetectedMKTT/RV + XLD + BGA 37MT0013

DetectedVIDASMT0022

DetectedBroth 37/agar 37 (Various)MT0044


DetectedPCRMT0052


DetectedVIDASMT4040

DetectedOtherMT4065

DetectedPCRMT4065

DetectedVIDASMT4102


DetectedChromogenic agarMT4240

DetectedPCRMT4240




DetectedELISAMT4354

DetectedOtherMT4386



DetectedELISAMT4408

Not DetectedBroth 37/agar 37 (Various)MT4417


DetectedPCRMT4446

DetectedOtherMT4448

DetectedOtherMT4486

DetectedVIDASMT4486



DetectedOtherMT4578

DetectedPCRMT4589





DetectedPCRMT4775

DetectedOtherMT4779

DetectedOtherMT4824


DetectedPCRMT4836

DetectedVIDASMT4836


DetectedVIDASMT4903

DetectedOtherMT4953



DetectedOtherMT5047

DetectedPCRMT5047


DetectedOtherMT5072

DetectedPCRMT5077

DetectedPCRMT5104

DetectedVIDASMT5175



DetectedPCRMT5192


DetectedPCRMT5212

DetectedELISAMT5221


Not DetectedMKTT/RV + XLD + BGA 37MT5271

DetectedOtherMT5286






ResultMethodLab ID

DetectedPCRMT5322

DetectedOtherMT5328



DetectedVIDASMT5343


DetectedVIDASMT5415


DetectedOtherMT5584



DetectedPCRMT5652


DetectedELISAMT5716


DetectedRapid test (Various)MT5731

DetectedVIDASMT5731


DetectedPCRMT5781


DetectedOtherMT5812



DetectedVIDASMT5872

DetectedPCRMT5894

Not DetectedChromogenic agarMT5971



DetectedOtherMT6006

DetectedPCRMT6017

DetectedOtherMT6054

DetectedOtherMT6056



DetectedOtherMT6059

DetectedOtherMT6060

DetectedVIDASMT6074

DetectedOtherMT6075

DetectedOtherMT6082

DetectedOtherMT6084

DetectedVIDASMT6104

DetectedOtherMT6105

DetectedPCRMT6121

DetectedOtherMT6134


DetectedOtherMT6156

Result

Data Statistics

DetectedAssigned Value


97%Satisfactory

Methodology Summary

% SatisfactoryMethod

97%MKTT/RV + XLD + BGA 37

83%Broth 37/agar 37 (Various)

100%VIDAS

100%Rapid test (Various)

80%Chromogenic agar

100%PCR

100%ELISA

100%Other





Comments

The sample contained Salmonella Panama at an approximate inoculum level of 20 cfu/g. The assigned value is therefore Salmonellaspecies ‘Detected’.

Salmonella Panama is characteristic of the genus. It produces typical reactions in biochemical tests and on selective agars. It is a group DSalmonella and according to the Kauffmann-White Scheme is O antigen: 1, 9, 12; H phase 1 antigen: l, v; H phase 2 antigen: 1, 5.

Salmonellae are Gram-negative, non-spore forming rods that are oxidase negative and catalase positive, and are members of theEnterobacteriaceae family. Almost all Salmonella serotypes cause acute gastro-enteritis and account for a large proportion of all foodpoisoning outbreaks. The primary source of Salmonellae is the gastrointestinal tract of animals, the organism can be transmitted into themeat during slaughtering and processing.


Analyte: Listeria species

Sample: 737 - Meat Listeria (P/A)


ResultMethodLab ID


DetectedFraser + ALOA 37MT0013


DetectedOtherMT0032



DetectedFraser + PL 37MT0052





DetectedOtherMT4102

DetectedOtherMT4109

DetectedOtherMT4305


DetectedOtherMT4332

DetectedOtherMT4397





DetectedOtherMT4486

DetectedOtherMT4541

DetectedRapid L.monoMT4550










DetectedOtherMT4836


DetectedOtherMT4894

DetectedOtherMT4903


DetectedOtherMT5020


DetectedOtherMT5037


DetectedOtherMT5175

DetectedOtherMT5182

DetectedOtherMT5187



DetectedOtherMT5227




DetectedOtherMT5415


DetectedOtherMT5467



DetectedOtherMT5585

DetectedOtherMT5652





DetectedOtherMT5731


Analyte: Listeria species



ResultMethodLab ID




DetectedOtherMT5820

DetectedOtherMT5822

DetectedOtherMT5824




DetectedOtherMT5887

Not DetectedFraser + ALOA 37MT5899


DetectedOtherMT5915

DetectedOtherMT5930

DetectedOtherMT5989

DetectedOtherMT6026


DetectedOtherMT6027


DetectedOtherMT6074

DetectedOtherMT6075


DetectedOtherMT6104


DetectedOtherMT6140

Result

Data Statistics


88Number of Results

99%Satisfactory

Methodology Summary


97%Fraser + ALOA 37

100%Fraser + PL 37


100%Rapid L.mono

100%Other

Comments

The sample contained Listeria monocytogenes at an approximate inoculum level of 18 cfu/g. The assigned value is therefore Listeriaspecies ‘Detected’.

There are ten species of Listeria, the most clinically significant being Listeria monocytogenes, which is pathogenic for humans and animals.While other members of the Listeria genus are not usually implicated in disease, their presence indicates an increased risk of contaminationby Listeria monocytogenes.


Analyte: Listeria monocytogenes



ResultMethodLab ID



DetectedOtherMT0022


DetectedOtherMT0032



DetectedOtherMT0052




DetectedOtherMT4065

DetectedOtherMT4102

DetectedOtherMT4109


DetectedOtherMT4240




DetectedOtherMT4332

DetectedOtherMT4397





DetectedOtherMT4486

DetectedOtherMT4541










DetectedOtherMT4775



DetectedOtherMT4836


DetectedOtherMT4894

DetectedOtherMT4903

DetectedOtherMT4904


DetectedOtherMT5020


DetectedOtherMT5037


DetectedOtherMT5047

DetectedOtherMT5072

DetectedOtherMT5175

DetectedOtherMT5182

DetectedOtherMT5187



DetectedOtherMT5227




DetectedOtherMT5322

DetectedOtherMT5415



Analyte: Listeria monocytogenes



ResultMethodLab ID

DetectedOtherMT5467

DetectedOtherMT5479



DetectedOtherMT5585

DetectedOtherMT5652





DetectedOtherMT5731




DetectedOtherMT5812

DetectedOtherMT5820

DetectedOtherMT5822




DetectedOtherMT5887



DetectedOtherMT5915

DetectedOtherMT5930

Not DetectedOtherMT5971

DetectedOtherMT5989


DetectedOtherMT6027


DetectedOtherMT6074

DetectedOtherMT6075

DetectedOtherMT6084

DetectedOtherMT6104

DetectedOtherMT6105

Result

Data Statistics


98Number of Results

99%Satisfactory

Methodology Summary


100%Fraser + ALOA 37

100%Fraser + PL 37


100%Rapid L.mono

98%Other

Comments

The sample contained Listeria monocytogenes at an approximate inoculum level of 18 cfu/g. The assigned value is therefore Listeriamonocytogenes ‘Detected’.

There are ten species of Listeria, the most clinically significant being Listeria monocytogenes, which is pathogenic for humans and animals.While other members of the Listeria genus are not usually implicated in disease, their presence indicates an increased risk of contaminationby Listeria monocytogenes.


Sample: 738 - Meat Clost/Staph

Analyte: Clostridium perfringens


z' score*Result (cfu/g)MethodLab ID

-1.0864TSC PP 37MT0025

1.26470OtherMT0032

<10TSC PP 37MT0068

-0.55100TSC PP 37MT4306

-0.34120TSC PP 37MT4408

1.08400OtherMT4437

0.00160TSC PP 37MT4440

<10TSC PP 37MT4562

0.52250TSC PP 37MT4564

-0.8180TSC PP 37MT4593

0.92350TSC PP 37MT4664

-0.24130TSC PP 37MT4667

0OtherMT4733

0TSC PP 37MT4759

3.132,300OtherMT4759

0OtherMT4903

-1.6340TSC PP 37MT4904

-1.6938OtherMT4935

0.46236TSC PP 37MT4935

<10TSC PP 37MT4996

-0.34120TSC PP 37MT5019

-0.9770TSC PP 37MT5211

0.50246TSC PP 37MT5212

-1.7237TSC PP 37MT5221

-0.55100TSC PP 37MT5340

0.74300TSC PP 37MT5353

1.83760TSC PP 37MT5366

0.95360TSC PP 37MT5375

-0.6890TSC PP 37MT5384

-3.2510OtherMT5384

0.26200OtherMT5415

-1.6340TSC PP 37MT5459

2.962,000TSC PP 37MT5483

-0.8975TSC PP 37MT5504

1.19440TSC PP 37MT5568

1.45550TSC PP 37MT5592

-1.3750TSC PP 37MT5597

-1.9630OtherMT5652

2.861,827TSC PP 37MT5698

-0.55100OtherMT5703

0TSC PP 37MT5731

3.102,250TSC PP 37MT5820

2.461,300TSC PP 37MT5838

1.18436TSC PP 37MT5839

<10OtherMT5852

0TSC PP 37MT5862

<10OtherMT5872

1.21450OtherMT5915

<10OtherMT5967

1.55600TSC PP 37MT6065

-0.9770TSC PP 37MT6067

<10TSC PP 37MT6085

-1.3750TSC PP 37MT6106

<10OtherMT6120

<10TSC PP 37MT6120


The sample contained Clostridium perfringens at an assigned value of 160 cfu/g.

Approximately 36% of participants failed to detect the target organism in the sample. Due to this further quality control analysis was




Value

Data Statistics

55Number of Results


2.27 log10Mean

2.20 log10Median





Value

160 cfu/gAssigned Value


0.35 log10SDPA

0.37 log10Expanded SDPA

29 to 879 cfu/gSatisfactory Range

85.4%Satisfactory z' scores

7.3%Questionable z' scores

7.3%Unsatisfactory z' scores

Methodology Summary



87.537 to 22500.552.1672.73840TSC PP 37

77.810 to 23000.552.3027.27615Other

85.410 to 23000.592.201001455All

Comments





Approximately 36% of participants failed to detect the target organism in the sample. Due to this further quality control analysis wasperformed internally at LGC Standards. The target organism was detected in all samples analysed and enumeration results were in line withthe assigned value and satisfactory range. Participants failing to isolate and enumerate the target organism may wish to investigate thisfurther internally, and take into consideration the low assigned value.

Clostridium perfringens causes an intoxication form of food poisoning characterised by diarrhoea, stomach cramps, nausea, headache, andfever. Foods most commonly associated with Clostridium perfringens contamination are usually high protein foods such as meat and poultrystews, sauces, gravies, pies, and casseroles.

*Please note, participant performance for this analyte has been assessed using a z' score, rather than a z score, in order to account for themeasurement uncertainty of the assigned value which is not negligible when compared to the SDPA.



Analyte: Sulphite-reducing Clostridium



0.14245TSC PP 37MT0025

0.75400OtherMT0032

0.00218TSC PP 37MT0052

<10IS PP 37MT0068

-0.97100TSC PP 37MT4306

-1.1090OtherMT4397

-0.97100OtherMT4408

<100OtherMT4417

0.75400OtherMT4437

-0.46150IS PP 37MT4440

<10TSC PP 37MT4562

0.40300IS PP 37MT4564

-1.4170OtherMT4593

-1.6060IS PP 37MT4608

-2.1040OtherMT4608

-0.64130TSC PP 37MT4667

<40OtherMT4739

3.082,600IS PP 37MT4759

3.032,500OtherMT4759

0.87440TSC PP 37MT4775

<100TSC PP 37MT4810

0OtherMT4903

-2.1040OtherMT4904

-0.85110OtherMT4935

-3.8210TSC PP 37MT4996

0.31280OtherMT5019

2.752,000IS PP 37MT5107

-0.64130IS PP 37MT5211

0.15246OtherMT5212

-1.4667TSC PP 37MT5221

-0.11200IS PP 37MT5340

1.24590TSC PP 37MT5353

1.55760TSC PP 37MT5366

0.01220OtherMT5375

-0.46150TSC PP 37MT5384

-1.8350OtherMT5384

-0.11200OtherMT5415

-2.4630OPSP PP 37MT5459

-0.97100IS PP 37MT5504

0.17250OtherMT5568

0.40300IS PP 37MT5592

-1.4170TSC PP 37MT5597

2.641,827TSC PP 37MT5698

-0.97100OtherMT5703

2.972,380OtherMT5820

2.551,700IS PP 37MT5838

0.47318IS PP 37MT5839

<10TSC PP 37MT5852

0.81418TSC PP 37MT5862

0.17250OtherMT5915

1.21580TSC PP 37MT6065

0TSC PP 37MT6067

<10OtherMT6085

-1.8350OtherMT6106


The sample contained the sulphite-reducing Clostridia Clostridium perfringens at an assigned value of 218 cfu/g.




Value

Data Statistics

54Number of Results


2.32 log10Mean

2.34 log10Median





Value



0.35 log10SDPA





Methodology Summary



85.710 to 18270.572.3633.33418TSC PP 37

0.030 to 300.001.481.8501OPSP PP 37

72.760 to 26000.542.4822.22112IS PP 37

78.940 to 25000.452.3042.59423Other

77.810 to 26000.502.34100954All

Comments





Sulphite-reducing bacteria cause food spoilage by the production of hydrogen sulphide, which produces foul odours. The majority ofsulphite-reducing bacteria belong to the genus Clostridium.

Clostridium is a large genus consisting of Gram-positive spore-forming anaerobes. Clostridia are often found in soil. Some are responsiblefor human and animal disease, and others are associated with food spoilage. Clostridia are classified according to the shape and position oftheir spores, and by their physiological characteristics.



Analyte: Coagulase positive staphylococci



-0.227,450BP + RPF SP 37MT0025

0.1410,000OtherMT0032

0.3011,325BP SP 37MT0052

-2.301,400BP SP 37MT0068

0.1410,000BP + RPF SP 37MT4306

0.3712,000OtherMT4397

-0.605,500BP SP 37MT4408

-0.675,200BP SP 37MT4417

-0.645,300OtherMT4437

1.7035,000BP SP 37MT4440

-0.436,300OtherMT4548

-2.301,400BP SP 37MT4562

0.4713,000BP + RPF PP 37MT4564

-0.994,000BP SP 37MT4593

-0.725,000BP SP 37MT4608

-0.038,700OtherMT4608

0.4713,000BP + RPF SP 37MT4664

-0.217,500BP SP 37MT4667

-1.852,000BP + RPF SP 37MT4739

0.3712,000BP SP 37MT4759

0.1410,000OtherMT4759


0.8017,000BP + RPF SP 37MT4810


-0.237,400OtherMT4903

-0.476,100BP SP 37MT4904

-0.197,615OtherMT4935

-0.515,900OtherMT4996

-0.098,300BP SP 37MT5019

0.2611,000BP + RPF SP 37MT5107

0.1410,000BP SP 37MT5175

0.129,800BP SP 37MT5211

0.5814,182BP + RPF SP 37MT5212

-1.512,637BP SP 37MT5221

0.2611,000BP SP 37MT5340

0.3712,000BP + RPF SP 37MT5353

0.3712,000BP + RPF SP 37MT5366

0.6515,000BP + RPF SP 37MT5375

0.1410,000BP + RPF SP 37MT5384



-0.964,100BP + RPF SP 37MT5459

0.6515,000BP + RPF SP 37MT5483

-0.416,400BP SP 37MT5504

0.099,600BP SP 37MT5568


0.089,500BP SP 37MT5592

-0.854,500BP SP 37MT5597

0.6515,000BP + RPF SP 37MT5652

0.2110,545BP SP 37MT5698

-0.326,900OtherMT5703


0.3712,000BP SP 37MT5838

0.4312,600BP + RPF SP 37MT5839

0.5413,700BP SP 37MT5839

0.2210,650BP SP 37MT5852

-0.098,272BP + RPF PP 37MT5862

0.9719,400OtherMT5872

-0.336,800BP + RPF PP 37MT5898

-0.994,000OtherMT5915

0.3712,000BP + RPF PP 37MT6065

-1.772,145BP SP 37MT6067






-0.416,400BP SP 37MT6085

-0.307,000BP + RPF SP 37MT6106

0.019,000BP + RPF SP 37MT6120

0.008,900OtherMT6120

-0.108,200BP SP 37MT6120

Value

Data Statistics

67Number of Results


3.90 log10Mean

3.95 log10Median





Value



0.35 log10SDPA









Methodology Summary



92.61400 to35000

0.213.9140.3027BP SP 37

100.02000 to17000

0.144.0825.37017BP + RPF SP 37

100.02700 to11000

0.123.898.9606Petrifilm

100.06800 to13000

0.154.005.9704BP + RPF PP 37

100.04000 to19400

0.163.8819.4013Other

97.01400 to35000

0.193.95100067All

Comments

The sample contained Staphylococcus aureus at an assigned value of 8,900 cfu/g.

The most significant food borne coagulase positive staphylococcus is Staphylococcus aureus. Staphylococcus aureus can be present onthe skin, and in the nose and throat of healthy individuals with no ill effect. It is important to test for it in foods as it can be an indicator ofgeneral hygiene and be a causative agent of food poisoning.


Sample: 739 - Fish E.coli/Staph




-0.94200PCA SP 30MT0048

0.17490PCA PP 30MT0068

0.09460PCA PP 30MT4206

-0.08400OtherMT4208

-0.32330PCA SP 30MT4251

0.61700PetrifilmMT4262

3.547,400PCA SP 30MT4262


-0.01425PCA PP 30MT4342

0.12470PCA PP 30MT4440

0.01430PCA PP 30MT4448


-0.38315PCA PP 30MT4578

0.18495PCA SP 30MT4670

-0.44300OtherMT4894

-0.05410PCA PP 30MT5016


4.5717,000PCA PP 37MT5105

-0.02420PCA PP 30MT5142


-0.28340PCA PP 37MT5312

<1,000PCA PP 30MT5320

-0.66250PCA PP 30MT5345

1.061,000OtherMT5370

0.07450PCA PP 37MT5432

0.07450OtherMT5444

-0.26345OtherMT5502

-0.44300OtherMT5585

-0.44300PCA PP 30MT5585

0.48628OtherMT5603

0.42600PCA PP 30MT5656


-0.25350PCA PP 30MT5721

-0.94200PCA SP 30MT5729

-0.25350PCA SP 30MT5777

-0.14380PCA PP 30MT5780

-0.21360PCA PP 30MT5818

-0.40310OtherMT5822

-0.56273PCA PP 37MT5839

0.66727PCA SP 30MT5839

0.20500PCA PP 37MT5842

0.60690PCA PP 30MT5868

-0.57270OtherMT5887

-0.94200PCA SP 30MT5892

0.09458PCA SP 30MT5909

0.09460PCA PP 30MT6020

0.09459OtherMT6092

-0.82220OtherMT6101

0.82830TEMPOMT6110

0.54660PCA PP 30MT6138

-0.08400PCA PP 30MT6139

Value

Data Statistics

51Number of Results


2.70 log10Mean

2.63 log10Median









Value



0.35 log10SDPA





Methodology Summary



100.0250 to 6900.082.6337.25119PCA PP 30

88.9200 to 74000.362.5417.6509PCA SP 30

80.0273 to 170000.182.659.805PCA PP 37

83.3340 to 230000.192.7511.7606Petrifilm

100.0830 to 8300.002.921.9601TEMPO

100.0220 to 10000.162.5421.57011Other

94.0200 to 230000.162.63100151All

Comments

The sample contained Staphylococcus aureus and Escherichia coli at an assigned value of 427 cfu/g.

Total aerobic mesophilic counts are used to determine the general microbial population of a sample. Bacterial swabs performed on meattypically contain 102 to 104 bacteria per cm2, counts greater than 105 per cm2 may indicate poor hygiene.






-0.5040VRBGA PP 37MT0048

0.1970VRBGA PP 37MT0068

0.0060VRBGA PP 37MT4206

0.1970OtherMT4208

0.0864VRBGA PP 30MT4251

-0.3645VRBGA PP 37MT4342

0.0663VRBGA PP 37MT4440

-0.2350VRBGA PP 37MT4448


-0.5040VRBGA PP 30MT4578

-0.1155VRBGA PP 37MT4670

-0.2350OtherMT4894

-0.0259VRBGA PP 37MT5016

0.4687VRBGA PP 37MT5105

0.63100VRBGA PP 37MT5142


0.2875VRBGA PP 37MT5312

<10VRBGA PP 37MT5320

-0.5040OtherMT5328

-2.2210VRBGA PP 37MT5345

-0.3645OtherMT5370

-0.8630VRBGA PP 37MT5432

-1.0925OtherMT5502

0.0060OtherMT5585

0.5392OtherMT5603



0.0060VRBGA PP 37MT5729

-0.2350VRBGA PP 37MT5777

0.3680VRBGA PP 30MT5780

-0.6735VRBGA PP 37MT5818

-0.6735OtherMT5822

0.0060VRBGA PP 37MT5839

0.75110VRBGA PP 37MT5868

2.00300OtherMT5887


0.3680VRBGA PP 37MT5892

-1.3620VRBGA PP 37MT5909

1.49200VRBGA PP 37MT6020

-0.2350VRBGA PP 37MT6101



Value

Data Statistics

42Number of Results


1.79 log10Mean

1.78 log10Median





Value



0.35 log10SDPA









Methodology Summary



95.510 to 2000.161.7761.9426VRBGA PP 37

100.040 to 800.141.817.1403VRBGA PP 30

50.060 to 4000.192.489.5214Petrifilm

100.025 to 3000.221.7021.4309Other

92.110 to 4000.191.78100542All

Comments

The sample contained the Enterobacteriaceae Escherichia coli at an assigned value of 60 cfu/g.

Members of the Enterobacteriaceae family are widely found in soil, water, fruits, vegetables, grains, flowering plants and a wide range ofanimals, from insects to humans.






-0.8630TBX PP 37-44MT0048

-0.8630TBX PP 37-44MT0068

2.53460MPNMT4116

0.1065Chromogenic agarMT4206

0.1970OtherMT4208

-0.1354TBX PP 37-44MT4251

-2.2210TBX PP 37-44MT4262


0.3680Chromogenic agarMT4324

0.1065TBX PP 37-44MT4342

-0.4741TBX PP 37-44MT4440

0.3680TBX PP 37-44MT4448


0.86120OtherMT4566

-0.5040COLI ID PP 37-44MT4578

-0.5040VRBA PP 37-44MT4670

-0.8630OtherMT4894

-0.2350OtherMT5016


0.0462TBX PP 37-44MT5105

0.0060TBX PP 37-44MT5142

>110MPNMT5227


0.5493MPNMT5312

0.96130MPNMT5316

-4.222MPNMT5320

-0.3346MPNMT5328

-0.8630TBX PP 37-44MT5345

0.3479MPNMT5370

0.5493MPNMT5399


0.3479TBX PP 37-44MT5444

-0.4143TBX PP 37-44MT5462

-0.5040OtherMT5502

0.0060OtherMT5585

0.63100OtherMT5603

0.0060TBX PP 37-44MT5656


-0.2350TBX PP 37-44MT5729

0.5392MPNMT5772

5.053,500MPNMT5773

0.5392TBX PP 37-44MT5774

0.5392MPNMT5775

1.22160MPNMT5777


-1.0925TBX PP 37-44MT5818


0.0060TBX PP 37-44MT5839

-1.3620TBX PP 37-44MT5842

-0.2350TBX PP 37-44MT5868

0.75110MPNMT5887

0.63100OtherMT5892

-1.4918TBX PP 37-44MT5909

0.75110MPNMT5963

0.5392MPNMT5965

0.63100TBX PP 37-44MT6020

-0.8630OtherMT6092

-0.1354MPNMT6101

0.3378OtherMT6110

<10PetrifilmMT6138

<10PetrifilmMT6139

-0.1354MPNMT6155





Value

Data Statistics

62Number of Results


1.76 log10Mean

1.78 log10Median





Value



0.35 log10SDPA









Methodology Summary



95.210 to 1000.291.7033.87021TBX PP 37-44

100.040 to 400.001.601.6101COLI ID PP 37-44

100.040 to 400.001.601.6101VRBA PP 37-44

83.330 to 30000.301.8412.928Petrifilm

81.32 to 4600.121.9627.42217MPN

75.010 to 800.321.656.4504Chromogenicagar

100.030 to 1200.281.8116.13010Other

89.82 to 30000.281.78100462All

Comments

The sample contained Escherichia coli at an assigned value of 60 cfu/g.

Escherichia coli is found in animal intestines, therefore the presence of the organism in meat indicates poor hygiene practice as the meatmay have become contaminated during the slaughtering process. It is also historically used as an 'indicator' organism, to indicate whethermore serious pathogens, such as Salmonella are also likely to be present. However, this organism is also widespread throughout theenvironment, being isolated from air, soils, water, plants and animals. Therefore the presence of Escherichia coli in meat may be faecal orenvironmental in origin.






0.13300BP SP 37MT0048

<10BP SP 37MT0068

0.49400BP SP 37MT4206

-0.10250OtherMT4208

0.29340BP SP 37MT4251

0.13300OtherMT4262


0.02275BP + RPF SP 37MT4324

<10BP SP 37MT4342

0.42380BP + RPF PP 37MT4440

0.55420BP + RPF PP 37MT4448


0.69470OtherMT4566

0.49400OtherMT4578

-1.3690BP + RPF SP 37MT4670

0.36360OtherMT4894

0.21320BP SP 37MT5016

-0.37200BP SP 37MT5047


0.25330BP SP 37MT5105


>110OtherMT5227

-0.15240OtherMT5228


0.49400BP + RPF PP 37MT5312

-3.2320BP SP 37MT5320

0.13300BP + RPF SP 37MT5328

-0.10250BP SP 37MT5345

1.18700OtherMT5370

0.42380BP SP 37MT5399

-0.81140BP + RPF SP 37MT5432

-0.20230OtherMT5444

-0.05260BP + RPF PP 37MT5462

-0.20230OtherMT5502

-0.31210BP + RPF PP 37MT5585

0.59433OtherMT5603

-1.23100BP SP 37MT5656

2.552,100OtherMT5661

-1.11110BP + RPF SP 37MT5721

-0.10250BP SP 37MT5729

-0.31210BP + RPF SP 37MT5775

-0.57170BP SP 37MT5775

0.00270BP + RPF PP 37MT5777

-3.2320BP + RPF PP 37MT5780


-0.07256OtherMT5822

-0.32209BP + RPF SP 37MT5839

-0.37200BP SP 37MT5839

-0.50180BP SP 37MT5842

0.42380BP SP 37MT5868

0.05280OtherMT5887

-0.73150BP + RPF SP 37MT5892

<10BP SP 37MT5909

-1.23100BP SP 37MT6020

-2.7330OtherMT6092

-0.73150BP SP 37MT6101

0.00270OtherMT6110

0.86540BP SP 37MT6138

0.05280BP SP 37MT6139


The sample contained Staphylococcus aureus at an assigned value of 270 cfu/g.




Value

Data Statistics

59Number of Results


2.38 log10Mean

2.43 log10Median





Value



0.35 log10SDPA





Methodology Summary



94.720 to 5400.212.4037.29322BP SP 37

100.090 to 3000.222.2513.5608BP + RPF SP 37

100.0260 to 4000.052.4610.1706Petrifilm

85.720 to 4200.222.4311.8607BP + RPF PP 37

86.730 to 21000.132.4527.12116Other

92.720 to 21000.192.43100459All

Comments





The most significant food borne coagulase positive staphylococcus is Staphylococcus aureus. Staphylococcus aureus can be present onthe skin, and in the nose and throat of healthy individuals with no ill effect. It is important to test for it in foods as it can be an indicator ofgeneral hygiene and be a causative agent of food poisoning.


The sample contained Salmonella Hadar at an approximate inoculum level of 5 cfu/g. The sample also contained Escherichia coli at anapproximate inoculum level of 2 cfu/g. The assigned value is therefore Salmonella species ‘Detected’.

Salmonella Hadar is characteristic of the genus on selective media. The strain is H2S positive, motile, lactose negative, citrate positive andurease negative. It is a group C2 Salmonella according to the Kauffmann-White Scheme, with a full serological profile of ‘O’ antigen: 6, 8; ‘H’


Sample: 740 - Fish Salmonella P/A


ResultMethodLab ID

DetectedVIDASMT4055

DetectedVIDASMT4208




DetectedELISAMT4408





DetectedOtherMT4898

DetectedPCRMT4898

DetectedVIDASMT4904


DetectedVIDASMT5003



DetectedPCRMT5322

DetectedVIDASMT5345

DetectedVIDASMT5353



DetectedVIDASMT5496

DetectedRapid test (Various)MT5502


DetectedELISAMT5716




DetectedPCRMT5930






DetectedOtherMT6092

DetectedVIDASMT6092




Result

Data Statistics


40Number of Results

98%Satisfactory

Methodology Summary


100%MKTT/RV + XLD + BGA 37


100%VIDAS

100%Rapid test (Various)

100%Chromogenic agar

100%PCR

100%ELISA

67%Other

Comments



Sample: 740 - Fish Salmonella P/A


urease negative. It is a group C2 Salmonella according to the Kauffmann-White Scheme, with a full serological profile of ‘O’ antigen: 6, 8; ‘H’phase 1 antigen: z10 and ‘H’ phase 2 antigen: e, n, x.

Salmonellae are Gram-negative, non-spore forming rods that are oxidase negative and catalase positive, and are members of theEnterobacteriaceae family. Almost all Salmonella serotypes cause acute gastro-enteritis and account for a large proportion of all foodpoisoning outbreaks. The primary source of Salmonellae is the gastrointestinal tract of animals, the organism can be transmitted into themeat during slaughtering and processing.


The sample contained Campylobacter hyointestinalis at an approximate level of 133 cfu/g. The assigned value is therefore Campylobacterspecies ‘Detected’.

Campylobacter species are Gram-negative rods that are highly motile, catalase and oxidase positive, do not ferment or oxidise sugars, andreduce nitrate to nitrite. They grow best in 5 to 10% oxygen, and have an optimum growth temperature of 37°C, although Campylobacterjejuni and Campylobacter coli are termed the thermophilic campylobacters, due to their ability to grow at 42°C.

Campylobacter species are present in the intestinal tract of a large number of wild and domestic animals, consequently, they are also found

Analyte: Campylobacter species

Sample: 743 - Meat Campylobacter species


ResultMethodLab ID

DetectedOtherMT0032

DetectedBOLTON + CCDA 42MT0048


Not DetectedBOLTON + CCDA 42MT0068

DetectedPRESTON + CCDA 42MT4040

DetectedOtherMT4095





DetectedBOLTON + KARM 42MT4508


Not DetectedBOLTON + CCDA 42MT4667


DetectedBOLTON + KARM 42MT4733

Not DetectedBOLTON + KARM 42MT4757

DetectedOtherMT4789


DetectedOtherMT4889

DetectedOtherMT4906






DetectedOtherMT5141

DetectedOtherMT5195



DetectedOtherMT5228

DetectedOtherMT5229






DetectedOtherMT5796

DetectedOtherMT5930


DetectedOtherMT6083


Result

Data Statistics


41Number of Results

88%Satisfactory

Methodology Summary


89%BOLTON + CCDA 42

67%BOLTON + KARM 42

100%PRESTON + CCDA 42

86%Other

Comments


Analyte: Campylobacter species

Sample: 743 - Meat Campylobacter species


Campylobacter species are present in the intestinal tract of a large number of wild and domestic animals, consequently, they are also foundto occur in untreated waters, and can contaminate seafood. Under-cooked poultry and food is thought to be the principle vehicle oftransmission. Raw and inadequately heat-treated milk products have also been responsible for a number of outbreaks.


The sample contained Escherichia coli O157 (non-toxigenic strain) at an approximate level of 16 cfu/g. The assigned value is therefore

Analyte: Escherichia coli O157 (non-toxigenic)

Sample: 744 - Presence/absence of Escherichia coli O157


ResultMethodLab ID

DetectedELISAMT0012

DetectedOtherMT0012

DetectedELISAMT0048

DetectedBROTH + CT-SMACMT0068

DetectedIMS + CT-SMACMT4040

DetectedVIDASMT4566

DetectedOtherMT4733


DetectedOtherMT5047

DetectedPCRMT5047


DetectedOtherMT5104

DetectedOtherMT5131

DetectedVIDASMT5227

DetectedPCRMT5233



DetectedVIDASMT5343


DetectedVIDASMT5382

DetectedOtherMT5504


DetectedVIDASMT5573

DetectedPCRMT5652

DetectedELISAMT5716

DetectedVIDASMT5796

DetectedOtherMT5812


DetectedOtherMT5890

DetectedPCRMT5911

DetectedELISAMT6008

DetectedOtherMT6008

DetectedPCRMT6008

DetectedOtherMT6027

DetectedVIDASMT6045

DetectedVIDASMT6046

DetectedOtherMT6075

DetectedPCRMT6075

DetectedOtherMT6094

DetectedVIDASMT6104

DetectedPCRMT6105

DetectedPCRMT6111

DetectedOtherMT6112

DetectedPCRMT6121

DetectedVIDASMT6131

Result

Data Statistics


45Number of Results

100%Satisfactory

Methodology Summary


100%BROTH + CT-SMAC

100%IMS + CT-SMAC

100%PCR

100%ELISA

100%VIDAS

100%Other

Comments


Analyte: Escherichia coli O157 (non-toxigenic)

Sample: 744 - Presence/absence of Escherichia coli O157


The sample contained Escherichia coli O157 (non-toxigenic strain) at an approximate level of 16 cfu/g. The assigned value is thereforeEscherichia coli O157 ‘Detected’.

Escherichia coli belongs to the Enterobacteriaceae family and the coliform group of indicator organisms. Cells of this organism are typicallyGram-negative rods that are motile, oxidase negative, produce gas from lactose at 37 and 44°C, possess the enzyme β-glucuronidase andproduce indole from tryptophan at 44°C. Some strains of Escherichia coli O157, in contrast, do not grow at 44°C and do not possess theβ-glucuronidase enzyme, so their presence may not be detected on chromogenic agars and in standard laboratory isolation procedures.Differentiation of Escherichia coli O157 from most other strains of Escherichia coli can usually be achieved using sorbitol MacConkey agar,as most strains of Escherichia coli O157 do not ferment sorbitol. There are also commercially available kits for confirmation of suspectcolonies, including latex agglutination kits.


Analyte: Vibrio species

Sample: 745 - Presence/absence of Vibrio species


ResultMethodLab ID


DetectedAPW + TCBS 37MT0068


Not DetectedAPW + TCBS 37MT4125






DetectedOtherMT4813


DetectedOtherMT4945











DetectedOtherMT5480

DetectedOtherMT5496



DetectedOtherMT5796






DetectedOtherMT5898




Result

Data Statistics


36Number of Results

78%Satisfactory

Methodology Summary


76%APW + TCBS 37

86%Other

Comments

The sample contained Vibrio parahaemolyticus at an approximate level of 5 cfu/g. The assigned value is therefore Vibrio species ‘Detected’.

Vibrio species are Gram-negative curved motile rods. They are usually found in aquatic habitats with a wide range of salinities, as sodiumions stimulate growth. Due to their presence in marine environments, they are commonly isolated from the surfaces and the intestinalcontents of marine animals. There are eight species of Vibrio that are pathogenic and have been shown to be associated with food. Vibrioparahaemolyticus, is known to cause food poisoning.


Analyte: Vibrio parahaemolyticus

Sample: 745 - Presence/absence of Vibrio species


ResultMethodLab ID












DetectedOtherMT4813

DetectedOtherMT4945





DetectedOtherMT5047







DetectedOtherMT5314




DetectedOtherMT5480

DetectedOtherMT5496




DetectedOtherMT5796





DetectedOtherMT5898



Result

Data Statistics


41Number of Results

83%Satisfactory

Methodology Summary


81%APW + TCBS 37

89%Other

Comments

The sample contained Vibrio parahaemolyticus at an approximate level of 5 cfu/g. The assigned value is therefore Vibrio parahaemolyticus‘Detected’.

Vibrio parahaemolyticus is a marine organism widely distributed in temperate and warm coastal waters. Therefore, it is usually only presentin the coastal waters of the UK in the warmer months. Vibrio parahaemolyticus food poisoning is usually associated with inadequatelycooked or raw seafood. This is one of the most frequent causes of food poisoning in Japan, where seafood contributes to a largeproportion of the diet. The organism is halophilic, having high salt requirements, and cannot, therefore, grow on CLED with andradeindicator. The organism is sucrose negative and therefore produces green-blue colonies on TCBS agar. It is resistant to 10µg of 0/129 butsensitive to 150µg of 0/129.


Sample: 746 - Pseudomonas/Lactic Acid/Yeast/Mould

Analyte: Pseudomonas species



0.032,300CF SP 25MT0022

0.132,500OtherMT0032

-0.281,800CF SP 25MT0048

0.754,100OtherMT0061

-1.87500CF SP 25MT0068

-1.45700CF SP 25MT4040

0.272,800CF SP 25MT4093

1.437,100CN SP 25MT4125

0.082,400CF SP 25MT4173

-0.351,700CF SP 25MT4291

-0.531,470CF SP 25MT4292

0.623,700CF SP 25MT4328

0.784,200OtherMT4328

0.653,800OtherMT4333

-0.042,184CF SP 25MT4333

0.403,100CF SP 25MT4334

0.553,500CF SP 25MT4373

0.132,500OtherMT4397

0.182,600CF SP 25MT4417

-0.891,100CF SP 25MT4541

0.132,500OtherMT4593

-7.585CF SP 25MT4733

-2.50300OtherMT4758

0.513,400OtherMT4761

-1.14900CF SP 25MT4778

-2.78240CF SP 25MT4889

-2.17390OtherMT4903

-0.032,200CF SP 25MT4904

-0.351,700OtherMT4923

-0.871,118CF SP 25MT4935

-2.31350CF SP 25MT4994

-0.152,000CF SP 25MT5012

-0.281,800CN SP 25MT5012

0.714,000OtherMT5017

0.553,500CF SP 25MT5020

0.082,400CF SP 25MT5141

-0.781,200CF SP 25MT5142

-0.351,700OtherMT5182

-0.032,200CF SP 25MT5228

-0.681,300CF SP 25MT5229

-0.501,500CF SP 25MT5342

0.974,900CF SP 25MT5343

-0.211,900CF SP 25MT5345

0.232,700CF SP 25MT5382

0.483,300CF SP 25MT5384

0.403,100OtherMT5400

-0.381,659OtherMT5480

0.082,400OtherMT5504

0.232,700CF SP 25MT5514

-1.02990OtherMT5691

-0.231,864CF SP 25MT5698

0.042,320CF SP 25MT5729

0.032,300CF SP 25MT5807

-4.7250OtherMT5822

<10OtherMT5967

0.784,200CF SP 25MT5969

-0.032,200CF SP 25MT6052

0.032,300OtherMT6052

0.132,500CF SP 25MT6065

-2.19385CF SP 25MT6067


The sample contained Pseudomonas aeruginosa at an assigned value of 2,249 cfu/g.

Pseudomonads are Gram-negative, motile, non-sporing rods. They are catalase and oxidase positive and utilise glucose oxidatively.




Value

Data Statistics

60Number of Results


3.25 log10Mean

3.35 log10Median





Value



0.35 log10SDPA





Methodology Summary



89.7240 to 49000.193.3465139CF SP 25

100.01800 to 71000.443.553.3302CN SP 25

83.350 to 42000.243.3931.67119Other

88.150 to 71000.213.35100260All

Comments





Pseudomonads are Gram-negative, motile, non-sporing rods. They are catalase and oxidase positive and utilise glucose oxidatively.Pseudomonas species are widespread in the soil, and their ability to reduce nitrate makes them an important part of the nitrogen cycle.They are also ubiquitous in water, sewage and on plants and animals, and in food. They are also a predominant organism in biofilms.Their widespread occurrence is due to their metabolic diversity. Pseudomonas species can be psychrotrophic and are commonly found inmilk stored at or below 7°C. They are common spoilage organisms in a variety of foods, especially bacon, fish and meats.



Analyte: Lactic acid bacteria


Result (cfu/g)MethodLab ID

4,500MRS SP 30MT0022

7,900OtherMT0032

<10MRS PP 25MT0048

<10MRS PP 30MT0061

5,500MRS SP 30MT0068

3,300MRS PP 30MT4040

<10MRS PP 30MT4093

6,500MRS SP 30MT4125

<10MRS PP 30MT4173

<100MRS PP 25MT4291

<10MRS SP 30MT4292

0MRS PP 30MT4328

<100OtherMT4333

<10MRS PP 25MT4333

6,680MRS SP 30MT4334

<10OtherMT4373

<100OtherMT4397

10,500MRS SP 30MT4417

1,800MRS SP 30MT4541

<10MRS PP 25MT4593

2,900OtherMT4733

<100OtherMT4758

<10MRS SP 30MT4761

<10MRS PP 30MT4778

2,500MRS PP 30MT4836

3,300OtherMT4894

6,700OtherMT4903

3,600MRS PP 30MT4904

480,000OtherMT4923

<10MRS SP 30MT4935

0MRS SP 30MT4994

0MRS SP 30MT5012

5,000OtherMT5017

<10OtherMT5072

<100MRS SP 30MT5141

0MRS PP 30MT5142

<10OtherMT5182

3,855MRS PP 30MT5207

6,700MRS PP 30MT5228

<10MRS PP 30MT5229

4,300MRS PP 30MT5342

9,500MRS PP 30MT5343

6,700MRS PP 30MT5345

9,300MRS PP 30MT5382

10,000MRS PP 30MT5384

18,000OtherMT5384

<200MRS SP 30MT5400

12,182OtherMT5480

400MRS SP 30MT5504

3,800MRS PP 30MT5514

<100PetrifilmMT5691

<10MRS PP 30MT5698

<100MRS SP 30MT5729

0MRS SP 30MT5807

<10MRS SP 30MT5969

9,400OtherMT5986

6,100MRS PP 30MT6065

1,655OtherMT6067

9,600OtherMT6135



Analyte: Lactic acid bacteria


Value

Data Statistics

59Number of Results

N/ANumber of Excluded Results

N/AMean

N/AMedian

N/AStandard Deviation

N/ARobust Standard Deviation

0 to 480,000 cfu/gResult Range


Value

AbsentAssigned Value

N/AUncertainty of Assigned Value

N/ASDPA

N/ASatisfactory Range

N/ASatisfactory z scores

N/AQuestionable z scores

N/AUnsatisfactory z scores

Comments

The sample did not contain Lactic acid bacteria. The assigned value is therefore ‘Absent’ using a detection level of <10 cfu/g.

Approximately 51% of participants incorrectly reported the presence of Lactic acid bacteria. It is likely that the strain of yeast present in thetest material will grow on agars intended for the enumeration of Lactic acid bacteria. Correct identification is therefore dependent uponsufficient confirmatory tests. Participants may wish to consider the significance of misidentifying the target organism.

Lactic acid bacteria share a number of common features, they are Gram-positive, non-spore forming rods or cocci, most are aerotolerantanaerobes which lack cytochromes and porphyrins, and are therefore also usually catalase and oxidase-negative. The principal genera areLactococcus, Leuconostoc, Pediococcus, Lactobacillus and Streptococcus. Lactic acid bacteria are important in the food, brewing and dairyindustries, where they are used in fermentations due to the production of lactic and acetic acids. They are beneficial to health and areincluded in a number of probiotic foods. However, lactic acid bacteria can also cause food spoilage, as high levels cause off flavours andodours and lead to a reduced shelf life. Lactic acid bacteria are often inhibitory to other micro-organisms.



Analyte: Yeast




0.399,300OtherMT0032

-0.225,700YGC SP 22-25MT0048

0.4810,000YGC SP 22-25MT0061

-0.693,900DRBC SP 22-25MT0068

-0.385,000RB SP 22-25MT4040

0.318,700DRBC PP 22-25MT4093

0.047,000YGC SP 22-25MT4125

-0.434,800DRBC PP 22-25MT4173

0.127,500YGC SP 22-25MT4291

-0.604,200OtherMT4292

0.8013,000DRBC SP 22-25MT4328

0.4810,000OGYE PP 22-25MT4328

-0.365,100OtherMT4328

0.9014,000OtherMT4333

0.5110,264YGC SP 22-25MT4333

0.359,000DRBC SP 22-25MT4334

0.177,800OtherMT4373

-0.086,400OtherMT4397

0.8013,000YGC SP 22-25MT4417

-3.87300OtherMT4541

-0.903,300DRBC SP 22-25MT4564

-1.192,600OtherMT4593

0.268,400OtherMT4733

-0.434,800OtherMT4758

0.208,000OtherMT4761

-1.581,900DRBC SP 22-25MT4778

0.389,200OtherMT4836

0.258,300DRBC SP 22-25MT4883

0.097,300DRBC SP 22-25MT4889

0.4810,000OtherMT4894

1.1016,455YGC SP 22-25MT4903

0.258,300OGYE PP 22-25MT4904

0.419,500OtherMT4923

-0.524,455DRBC SP 22-25MT4935

-2.46936OtherMT4935

-0.813,548YGC SP 22-25MT4935

-0.693,900YGC SP 22-25MT4994

-0.943,200OGYE SP 22-25MT5012

-0.943,200DRBC SP 22-25MT5012

-0.664,000OtherMT5017

0.7912,900DRBC SP 22-25MT5020

0.006,800OtherMT5072

-0.265,500YGC SP 22-25MT5141

-0.414,900DRBC PP 22-25MT5142

-0.693,900DRBC SP 22-25MT5172

0.228,100OtherMT5182

-0.414,880DRBC SP 22-25MT5207

0.9815,000DRBC SP 22-25MT5228

1.2118,000OtherMT5228

-0.464,700DRBC SP 22-25MT5229

-0.205,800OGYE PP 22-25MT5229

0.117,455OtherMT5321

-0.056,545DRBC SP 22-25MT5321

-0.166,000RB SP 22-25MT5342

-0.086,400DRBC SP 22-25MT5343

-0.086,400RB SP 22-25MT5343

-0.434,800DRBC SP 22-25MT5345

-0.096,300OGYE PP 22-25MT5382

0.9815,000OtherMT5384


0.4810,000YGC SP 22-25MT5400



Analyte: Yeast



0.409,364OtherMT5480

-0.046,600OtherMT5504

0.359,000DRBC SP 22-25MT5514

-0.315,300DRBC SP 22-25MT5656


-0.175,909DRBC SP 22-25MT5698

0.7011,910YGC SP 22-25MT5729


-0.693,900OtherMT5807

0.8313,272DRBC SP 22-25MT5862

0.439,600DRBC SP 22-25MT5969


0.097,300DRBC SP 22-25MT6065

-1.751,664YGC SP 22-25MT6067


Value

Data Statistics

77Number of Results


3.81 log10Mean

3.83 log10Median





Value



0.35 log10SDPA







Analyte: Yeast


Methodology Summary



100.03200 to 32000.003.511.301OGYE SP 22-25

100.05000 to 64000.043.783.903RB SP 22-25

100.01900 to15000

0.223.8129.87023DRBC SP 22-25

100.04800 to 87000.013.693.903DRBC PP 22-25

100.05800 to10000

0.123.865.1904OGYE PP 22-25

100.01664 to16455

0.203.8816.88013YGC SP 22-25

100.06100 to18000

0.143.897.7906Petrifilm

91.7300 to 180000.163.8831.17024Other

97.4300 to 180000.223.83100077All

Comments

The sample contained the yeast Pichia fermentans at an assigned value of 6,800 cfu/g.

Fungi are ubiquitous throughout the environment, being found in air, water, soils, animals and foods. They can survive adverse conditionsby the production of spores. Visible mould growth can often be seen on foods, such as bread and vegetables, and in the environment, suchas in bathrooms, air conditioning units, etc. Yeasts are simply fungi whose main growth form is unicellular and which usually replicate bybudding. Many yeasts can also grow in the hyphal form, and some moulds can be yeast-like. The distinction between a yeast and a mouldcan, therefore, be difficult. Many fungi are saprophytes, causing spoilage of foods and other commodities. Some are plant pathogens, andothers are recognised causes of human disease.



Analyte: Mould




0.283,500OtherMT0032

-0.302,200YGC SP 22-25MT0048

0.313,600YGC SP 22-25MT0061

-1.051,200DRBC SP 22-25MT0068

0.313,600RB SP 22-25MT4040

0.093,000DRBC PP 22-25MT4093

0.042,900YGC SP 22-25MT4125

-0.142,500DRBC PP 22-25MT4173

0.133,100YGC SP 22-25MT4291

0.093,000OtherMT4292

0.694,900DRBC SP 22-25MT4328

0.313,600OGYE PP 22-25MT4328

0.644,700OtherMT4328

0.413,900OtherMT4333

-0.601,727YGC SP 22-25MT4333

0.293,530DRBC SP 22-25MT4334

-0.422,000OtherMT4373

-0.092,600OtherMT4397

0.002,800YGC SP 22-25MT4417

-0.621,700OtherMT4541

0.093,000DRBC SP 22-25MT4564

0.313,600OtherMT4593

-0.242,300OtherMT4733

-0.551,800OtherMT4758

0.353,700OtherMT4761

-0.192,400DRBC SP 22-25MT4778

-1.281,000OtherMT4836

-0.052,700DRBC SP 22-25MT4883

-0.142,500DRBC SP 22-25MT4889

0.383,800OtherMT4894

-0.142,500YGC SP 22-25MT4903

-0.362,100OGYE PP 22-25MT4904

0.444,000OtherMT4923

0.323,619DRBC SP 22-25MT4935

-0.651,655OtherMT4935

-0.621,703YGC SP 22-25MT4935

-0.861,400YGC SP 22-25MT4994

0.002,800OGYE SP 22-25MT5012

0.093,000DRBC SP 22-25MT5012

0.413,900OtherMT5017

0.133,100DRBC SP 22-25MT5020


-0.142,500YGC SP 22-25MT5141

-0.192,400DRBC PP 22-25MT5142

-0.951,300DRBC SP 22-25MT5172

0.413,900OtherMT5182

-0.671,635DRBC SP 22-25MT5207

-0.422,000DRBC SP 22-25MT5228

0.444,000OtherMT5228

-1.051,200DRBC SP 22-25MT5229

-1.33960OGYE SP 22-25MT5229

-0.601,727OtherMT5321

-0.162,455DRBC SP 22-25MT5321

0.173,200RB SP 22-25MT5342

0.504,200DRBC SP 22-25MT5343

0.504,200RB SP 22-25MT5343

3.5046,900DRBC SP 22-25MT5345

0.313,600OGYE PP 22-25MT5382

-0.092,600OtherMT5384


0.996,200YGC SP 22-25MT5400



Analyte: Mould



-0.012,773OtherMT5480

0.173,200OtherMT5504

0.674,800DRBC SP 22-25MT5514


-0.671,636DRBC SP 22-25MT5698

0.213,320YGC SP 22-25MT5729


0.093,000OtherMT5807

0.233,363DRBC SP 22-25MT5862

-6.8711OtherMT5967

0.956,000DRBC SP 22-25MT5969


0.042,900DRBC SP 22-25MT6065

-2.16490YGC SP 22-25MT6067

<10PetrifilmMT6135

Value

Data Statistics

77Number of Results


3.42 log10Mean

3.45 log10Median





Value



0.35 log10SDPA







Analyte: Mould


Methodology Summary



100.0960 to 28000.343.212.602OGYE SP 22-25

100.03200 to 42000.083.563.903RB SP 22-25

95.51200 to46900

0.183.4728.57022DRBC SP 22-25

100.02400 to 30000.033.403.903DRBC PP 22-25

100.02100 to 36000.103.563.903OGYE PP 22-25

92.3490 to 62000.183.4016.88013YGC SP 22-25

100.01000 to 39000.243.259.0917Petrifilm

95.81000 to 47000.173.4831.17124Other

96.1490 to 469000.183.45100277All

Comments

The sample contained the mould Fusarium moniliforme at an assigned value of 2,800 cfu/g.

Fungi are ubiquitous throughout the environment, being found in air, water, soils, animals and foods. They can survive adverse conditionsby the production of spores. Visible mould growth can often be seen on foods, such as bread and vegetables, and in the environment, suchas in bathrooms, air conditioning units, etc. Yeasts are simply fungi whose main growth form is unicellular and which usually replicate bybudding. Many yeasts can also grow in the hyphal form, and some moulds can be yeast-like. The distinction between a yeast and a mouldcan, therefore, be difficult. Many fungi are saprophytes, causing spoilage of foods and other commodities. Some are plant pathogens, andothers are recognised causes of human disease.


Sample: 748 - Fish based sample

Analyte: Histamine


z scoreResult (mg/kg)MethodLab ID

2.87320.00VariousMT4094

-0.38191.89VariousMT4304

0.71235.15VariousMT4328

-1.65142.00VariousMT4337

-1.21159.43VariousMT4352

-1.68140.83VariousMT4388

0.68233.80VariousMT4550

1.11250.80VariousMT4736

0.06209.56VariousMT4821

-2.21120.00VariousMT4904

-0.03205.85VariousMT5211

0.25216.72VariousMT5265

0.66233.00VariousMT5365

-1.17160.99VariousMT5537

-0.24197.48VariousMT5538

-0.51187.00VariousMT5605

0.61231.02VariousMT5606

<7.70VariousMT5670

0.67233.58VariousMT5751

-0.30195.01VariousMT5796

0.80238.43VariousMT5852

0.71235.00VariousMT5860

2.18293.00VariousMT5980

2.18293.00VariousMT5981

-1.95130.00VariousMT5990

0.33220.00VariousMT5991

-0.05205.00VariousMT5992

0.63232.00VariousMT6004

0.51227.00VariousMT6048

0.53228.06VariousMT6050

0.00207.00VariousMT6053

-0.25197.00VariousMT6068

-0.84174.00VariousMT6069

1.45264.00VariousMT6070

-0.81175.00VariousMT6071

-1.33154.57VariousMT6091

-0.23198.09VariousMT6116

-0.47188.33VariousMT6117

Value

Data Statistics

38Number of Results


208.75 mg/kgMean

207.00 mg/kgMedian

44.825 mg/kgStandard Deviation

39.418 mg/kgRobust Standard Deviation

120.00 to 320.00 mg/kgResult Range


Value

207.00 mg/kgAssigned Value

8.10 mg/kgUncertainty of Assigned Value

39.418 mg/kgSDPA

128.16 to 285.84 mg/kgSatisfactory Range






Analyte: Histamine


Methodology Summary

%mg/kgTotalResultsResultsMethod


89.2120.00 to320.00

39.418207.00100138Various

89.2120.00 to320.00

39.418207.00100138All

Comments



Analyte: Total volatile nitrogen (TVN)


z scoreResult (mg/100g)MethodLab ID

-0.1844.53VariousMT4328

1.5057.00VariousMT4337

-0.2743.86VariousMT4352




-3.5319.68VariousMT5211

-1.7432.96VariousMT5265

-4.0116.10VariousMT5480


-0.2543.96VariousMT5538

-0.6041.39VariousMT5598

-0.1744.56VariousMT5605

-0.8239.78VariousMT5606


-0.1244.99VariousMT5796

-1.8032.53VariousMT5832

-0.0645.43VariousMT5847


-0.6541.00VariousMT5860



-1.0038.42VariousMT5978



-0.2344.14VariousMT5990






-0.6441.07VariousMT6050


-0.0345.60VariousMT6068



-0.0945.19VariousMT6100


-0.1844.52VariousMT6117

Value

Data Statistics

39Number of Results


46.72 mg/100gMean

45.85 mg/100gMedian

10.065 mg/100gStandard Deviation

7.415 mg/100gRobust Standard Deviation

16.10 to 60.91 mg/100gResult Range


Value

45.85 mg/100gAssigned Value

1.50 mg/100gUncertainty of Assigned Value

7.415 mg/100gSDPA

31.02 to 60.68 mg/100gSatisfactory Range






Analyte: Total volatile nitrogen (TVN)


Methodology Summary

%mg/100gTotalResultsResultsMethod


89.716.10 to60.91

7.41545.85100139Various

89.716.10 to60.91

7.41545.85100139All

Comments

One participant has reported a value which is approximately twice the general consensus which may be due to a calculation or dilution error.



Analyte: Trimethylamine (TMA)


z' score*Result (mg/100g)MethodLab ID

-1.431.88VariousMT4328







<10.00VariousMT5990


<10.00VariousMT6048


Value

Data Statistics

11Number of Results


5.87 mg/100gMean

6.72 mg/100gMedian

3.557 mg/100gStandard Deviation

3.099 mg/100gRobust Standard Deviation

0.60 to 11.37 mg/100gResult Range


Value

6.72 mg/100gAssigned Value

1.37 mg/100gUncertainty of Assigned Value

3.099 mg/100gSDPA

3.388 mg/100gExpanded SDPA

>0 to 13.50 mg/100gSatisfactory Range

88.9%Satisfactory z' scores

0.0%Questionable z' scores

11.1%Unsatisfactory z' scores

Methodology Summary

%mg/100gTotalResultsResultsMethod


88.90.60 to 11.373.0996.72100311Various

88.90.60 to 11.373.0996.72100311All

Comments

One very low result of 0.60 mg/100g was reported which is very low by comparison to the assigned value and may be due to a calculationerror or the result being reported in an alternative format to that requested.

Participants are reminded that all results should be reported in the appropriate format to ensure that a fair evaluation of the data can beprovided. Participants are also reminded that the reporting of results is considered an integral part of the proficiency test.

*Please note, participant performance for this analyte has been assessed using a z' score, rather than a z score, in order to account for themeasurement uncertainty of the assigned value which is not negligible when compared to the SDPA.


Documents

QMAS - Quality in Meat and Fish Analysis PT Scheme Round: 233siteform.tonynetcn.com/X_UpDown/10000852-D1015664ECBD2A21/... · [1] ISO 13528 (2005), ‘Statistical methods for use