Quality Matters: Stock Culture Maintenance Protocol A p p l i c a t i o n N o t e s Key Words Stock culture maintenance, microbiology, quality control organisms, storage, subculture, bacterial, fungal, reference strains, Culti-Loops Goal Detail suggested quality control organism stock culture maintenance and its impact on the performance of microbiology test results. Background Quality control organisms are a fundamental part of any quality assessment program for microbiology laboratories. To ensure organism viability, proper maintenance of these organisms is critical to achieving accurate control results for culture media and reagents. It is important to safeguard the purity and identity of quality control stock cultures by limiting the potential for contamination or alteration of growth characteristics due to excessive sub-culturing.* CLSI Standard, M22-A3, provides guidance for storage and processing of quality control organisms. A visual diagram outlining a recommended procedure for maintaining quality control stock and working cultures is shown on the following page. Always review local regulatory guidelines and test manufacturer directions for maintaining microorganisms. Custom Quality Control Organisms We will work with you to develop a custom quality control organism that suits your speciﬁc needs. Contact your local Technical Sales Representative to request more information. References: 1. CLSI M02-A10 Vol. 29 No. 1, Performance Standard for Antimicrobial Disk Susceptibility Tests; Approved Standard-Tenth Edition, January 2009. 2. CLSI M22-A3 Vol. 24 No. 19, Quality Control for Commercially Prepared Microbiological Culture Media: Approved Standard – Seventh Edition, April 2004. 3. CLSI M07-A8 Vol. 29 No. 2, Methods for Dilution Antimicrobial Susceptibility Testsfor Bacteria that Grow Aerobically: Approved Standard-Eighth Edition, January 2 009. *Due to the innate growth characteristics of some microorganisms, not all strains are suitable for stock culturing; these include, but are not limited to, anaerobic organisms, fastidious organisms, and organisms used for susceptibility testing. Self validation is required for stock cultures of these organism types.

QC Stock Culture Maintenance Protocol

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Page 1: QC Stock Culture Maintenance Protocol

8/15/2019 QC Stock Culture Maintenance Protocol

http://slidepdf.com/reader/full/qc-stock-culture-maintenance-protocol 1/2

Quality Matters:Stock Culture Maintenance Protocol

A p p l i c a t

i on N

o t e s

Key WordsStock culture maintenance, microbiology, quality control organisms,storage, subculture, bacterial, fungal, reference strains, Culti-Loops

GoalDetail suggested quality control organism stock culture maintenance and its

impact on the performance of microbiology test results.

BackgroundQuality control organisms are a fundamental part of anyquality assessment program for microbiology laboratories.To ensure organism viability, proper maintenance of theseorganisms is critical to achieving accurate control resultsfor culture media and reagents.

It is important to safeguard the purity and identity of

quality control stock cultures by limiting the potential forcontamination or alteration of growth characteristics dueto excessive sub-culturing.*

CLSI Standard, M22-A3, provides guidance for storageand processing of quality control organisms. A visualdiagram outlining a recommended procedure formaintaining quality control stock and working cultures isshown on the following page.

Always review local regulatory guidelines and testmanufacturer directions for maintaining microorganisms.

Custom Quality Control OrganismsWe will work with you to develop a custom qualitycontrol organism that suits your specic needs.Contact your local Technical Sales Representative torequest more information.

References:1. CLSI M02-A10 Vol. 29 No. 1, Performance Standard for Antimicrobial DiskSusceptibility Tests; Approved Standard-Tenth Edition, January 2009.2. CLSI M22-A3 Vol. 24 No. 19, Quality Control for Commercially PreparedMicrobiological Culture Media: Approved Standard – Seventh Edition, April 2004.3. CLSI M07-A8 Vol. 29 No. 2, Methods for Dilution Antimicrobial SusceptibilityTestsfor Bacteria that Grow Aerobically: Approved Standard-Eighth Edition,

January 2009.

*Due to the innate growth characteristics of some microorganisms, not all strains aresuitable for stock culturing; these include, but are not limited to, anaerobic organisms,fastidious organisms, and organisms used for susceptibility testing. Self validation isrequired for stock cultures of these organism types.

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Day 2 Day 3 Day 4 Day 5 Day 6 Day 7

Day 9 Day 10 Day 11 Day 12 Day 13 Day 14

Day 16 Day 17 Day 18 Day 19 Day 20 Day 21

Day 23 Day 24 Day 25 Day 26 Day 27 Day 28

Stock Culture Maintenance

Sub-culture colonies from the working cultureto a fresh agar plate each day

Sub-culture colonies fromthe stock culture to a fresh

agar plate each week

Day 1

ReferenceCulture

StockCulture

WorkingCulture

Day 8

WorkingCulture

Day 15

WorkingCulture

Day 22

WorkingCulture

Inoculate on a non-selective agar plate or slant

The ATCC Licensed Derivative Emblem, the ATCC Licensed Derivative word mark, and the ATCC catalog marks aretrademarks of ATCC. Thermo Fisher Scientic is licensed to use these trademarks and to sell products derived fromATCC ® cultures. Look for the ATCC Licensed Derivative ® Emblem for products derived from ATCC ® cultures.

Visit our Look-Up Tool at www.remel.com/support/qclookup.aspx and quickly browse by genus,species, and/or ATCC ® reference number.

thermoscientific.com/microbiology

991-078July 2014

Contact Information:

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USA+1 800 255 [email protected]