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C/ Tarragona, 106 · 08015 Barcelona · SPAIN · tel +34 93 226 44 77 · fax +34 93 226 79 79 ; e-mail: [email protected] · www.bioser.com Código Descripción Formato 025009050 Microgen Legionella latex 50 test 025010050 Microgen E.coli O:157 latex 50 test 025012050 Microgen Campylobacter latex 50 test 025014050 Microgen Salmonella latex 50 test 025016100 Microgen Staph latex 100 test 025018050 Microgen Strep latex 50 test 025015050 Microgen Listeria latex 50 test Bioser, S.A. le ofrece sistemas simples y de confianza para laboratorios clínicos, alimentarios y farmacéuticos. Pruebas de confirmación de microorganismos Código Descripción Formato 025022020 Microgen Listeria ID 20 test 025020060 Microgen GN A ID 60 test 025021024 Microgen GN B ID 24 test 025033020 Microgen Bacillus ID 20 test 025034020 Microgen Staph ID 20 test 025041020 Microgen Strep ID 20 test Características: Disponibles todos los reactivos necesarios Sustratos basados en las pruebas convencionales: correlación directa con métodos estándares. Software de Identificación con muchas funciones y fácil manejo Características: Resultados en dos minutos Eficiencia incrementada Elevada sensibilidad Elevada especificidad Resultados exactos y fiables Un solo paso Beneficios: Sistema flexible Ahorro en el coste Ahorro en el espacio Ahorro en el tiempo Fácil de usar Galerías de identificación Bioquímica Test de aglutinación de Latex

Pruebas de confirmación de microorganismos - …jornades.uab.cat/.../files/Microgen_ID_Microgen_latex.pdf · Pruebas de confirmación de microorganismos Código Descripción Formato

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Page 1: Pruebas de confirmación de microorganismos - …jornades.uab.cat/.../files/Microgen_ID_Microgen_latex.pdf · Pruebas de confirmación de microorganismos Código Descripción Formato

C/ Tarragona, 106 · 08015 Barcelona · SPAIN · tel +34 93 226 44 77 · fax +34 93 226 79 79 ; e-mail: [email protected] · www.bioser.com

Código Descripción Formato

025009050 Microgen Legionella latex 50 test

025010050 Microgen E.coli O:157 latex 50 test

025012050 Microgen Campylobacter latex 50 test

025014050 Microgen Salmonella latex 50 test

025016100 Microgen Staph latex 100 test

025018050 Microgen Strep latex 50 test

025015050 Microgen Listeria latex 50 test

Bioser, S.A. le ofrece sistemas simples y de confianza para laboratorios clínicos, alimentarios y farmacéuticos.

Pruebas de confirmación de microorganismos

Código Descripción Formato

025022020 Microgen Listeria ID 20 test

025020060 Microgen GN A ID 60 test

025021024 Microgen GN B ID 24 test

025033020 Microgen Bacillus ID 20 test

025034020 Microgen Staph ID 20 test

025041020 Microgen Strep ID 20 test

Características:

• Disponibles todos los reactivos necesarios

• Sustratos basados en las pruebas convencionales:

correlación directa con métodos estándares.

• Software de Identificación con muchas funciones

y fácil manejo

Características:

• Resultados en dos minutos

• Eficiencia incrementada

• Elevada sensibilidad

• Elevada especificidad

• Resultados exactos y fiables

• Un solo paso

Beneficios:

• Sistema flexible

• Ahorro en el coste

• Ahorro en el espacio

• Ahorro en el tiempo

• Fácil de usar

Galerías de identificación Bioquímica

Test de aglutinación de Latex

Page 2: Pruebas de confirmación de microorganismos - …jornades.uab.cat/.../files/Microgen_ID_Microgen_latex.pdf · Pruebas de confirmación de microorganismos Código Descripción Formato

Bioser, S.A. c/Tarragona 106 · 08015 Barcelona · Tel: 93 226 44 77 · Fax: 93 226 79 79 · [email protected] · www.bioser.com

Background The AOAC-RI validated Microgen Listeria-ID has been improved to make the system the easiest-to-read, user-friendly and cost-effective method of identifying Listeria species on the market today. The Microgen Listeria-ID is a biochemical identification system which consists of 10 sugar fermentation tests, Aesculin hydrolysis and an improved micro-haemolysis reaction. The system does not require any reagent additions and reduces laboratory costs whilst utilising substrates which conform to all international standards. Microgen Listeria-ID (product code: MID-67) has now been improved to provide the easiest to read method of differentiating haemolytic and non-haemolytic Listeria spp.. Haemolytic and non-haemolytic Listeria spp. have traditionally been differentiated by the sub-culture from selective medium to sheep blood agar for the detection of haemolytic activity or by performing the variable, complex and difficult to interpret CAMP test. The inclusion of a sensitive and stable micro-haemolysis in the Microgen Listeria-ID allows for the easy interpretation of haemolysis of the red blood cells by haemolytic Listeria spp.. Interpretation of the New Haemolysis Reaction The haemolysis reaction in the MID Listeria is based on the determination of the ability of a test organism to lyse the red blood cells contained in the haemolysis reagent contained with this test. A STRONG POSITIVE HAEMOLYSIS TEST is typically characterized by the complete lysis of all of the red blood cells contained within the haemolysis reagent. This is seen as the development of a cloudy straw brown suspension in the test well and the absence of a deposit or button of cells in the bottom of the test well.

Typical POSITIVE REACTION (Total haemolysis)

A WEAK POSITIVE HAEMOLYSIS TEST is typically characterized by the incomplete lysis of the red blood cells within the haemolysis reagent. This is seen as the development of a cloudy straw to dark brown suspension in the test well and the presence of a small deposit or button of cells in the bottom of the test well. Typical WEAK POSITIVE REACTION (Partial haemolysis)

Weak positive haemolysis tests may occur with strains of L. monocytogenes and L. seeligeri. Although L. monocytogenes generally produces a strong definitive haemolysis reaction, it is possible for a strain to show a weak reaction. This is normally when the organism has been stressed e.g. from environmental samples or if the strain has been resuscitated from storage. Weak haemolysis is typically seen in weak producers such as L. seeligeri. A NEGATIVE HAEMOLYSIS TEST is typically characterized by the absence of lysis of any of the red blood cells contained within the haemolysis reagent. This is seen as the development of a clear straw to very pale pink colour in the test well and the presence of a large opaque red to brown coloured deposit or button of cells in the bottom of the test well. Typical NEGATIVE REACTION (no haemolysis) Procedural note: Test strains that have been removed from storage (freeze dried or lenticules) should be considered to be ‘damaged’. Optimum activity needs to be restored by inoculation into a suitable broth (e.g. TSYB) and incubated for 18-24 hours at 37°C. if a haemolytic strain, the presence of haemolysin should be confirmed by inoculating a blood plate and incubating for 18-24 hours at 37°C.

Guidelines for the Interpretation of the New Haemolysis Test in the Microgen Listeria-ID