*PROTEINS vs. NUCLEIC ACIDS

- PROTEINS : Structural & Functional

- NUCLEIC ACIDS : Storage of information

The methods:

Computational

Experimental

The methods:

Computational

- Ab initio (from principles/from scracht)- Semi-empirical (from experiments) Experimental

The methods:

Computational

- Ab initio (from principles/from scracht)- Semi-empirical (from experiments) Experimental

!!! Interaction of matter with electromagnetic radiation: - EM - X-rays crystallography - NMR - CD

alamethicin

cytochrome c'

RNA-binding protein Rop

cro repressor from phage 434

Interleukin-2, human Growth Hormone, Granulocyte-macrophagecolony-stimulating factor (GM-CSF) and Interleukin-4.

homeodomain binding to DNA.

-sandwiches

-barrels

porin

SH2 domain

placental ribonuclease inhibitor

Ribonuclease-H

*PROTEINS vs. NUCLEIC ACIDS

*UNIQUENESS

*PROTEINS vs. NUCLEIC ACIDS

*UNIQUENESS

*MOLECULAR RECOGNITION EVENTS

http://www.chembio.uoguelph.ca/driguana/NMR/TOC.HTM

http://www.expasy.org/swissmod/course/text/chapter4.htm

http://www.rmn.ub.es

INTRODUCTION TO NMR:

*The concept of chemical shift

*Scalar coupling

Larmor equation: = Bo

Nupper -Bo/kT

= e Nlower

Larmor equation: = Bo