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Protein Synthesis Outline October 18, 2012
EQ
I) CentralDogmaofBiology:
DNA transcription RNA translation Protein
Protein Synthesis Outline October 18, 2012
Transcription
A) Tostarttranscription,manydifferent
proteinscometogetherandbindtoastrand
ofDNA.TheseproteinsincludeRNA
polymeraseandotherproteinsreferredto
astranscriptionfactors.Thesetranscription
factorsalsoincludehelicasetounwindthe
DNA.
Protein Synthesis Outline October 18, 2012
B) RNApolymeraseaddscomplementaryRNA
nucleotidestotheexposedDNAbases.
1) RNApolymeraserecognizesasequenceon
theDNAstrandcalledthepromoter
sequenceandbindstoit;thisisthestarting
pointfortranscription.
Protein Synthesis Outline October 18, 2012
2) RNApolymerasecanonlymoveinone
directiondowntheDNAstrand,soonlyone
sideoftheunwoundDNAisusedforthe
pairingofRNAnucleotides.Thisiscalledthe
templatestrand.
3) WhentheRNApolymerasereachesa
terminatorsequence,itstopstranscription
andthemRNAstrandisreleased.
Protein Synthesis Outline October 18, 2012
C) ThestrandofmRNAisreleasedfromthe
nucleus,passingthroughthenuclear
poresintothecytoplasm.
Protein Synthesis Outline October 18, 2012
III) Translation:“translating”themessage
fromthelanguageofnucleicacids
(nucleotides)tothelanguageofprotein
(aminoacids)
A) RibosomeattachestomRNA.
1) Ribosomesconsistof
twopieces,calledthe
largeandsmall
subunits.
2) Thesubunitsoftheribosome
surroundthemRNAstrand,muchlike
ahamburgerbunsurroundsthe
burger.
Protein Synthesis Outline October 18, 2012
B) Ribosomesreadcodonsoneatatime.
C) tRNAmoleculesbringaminoacidstothe
ribosome.
1) tRNAanticodonsarecomplementary
tothemRNAcodonsbeingreadbythe
ribosome.
Protein Synthesis Outline October 18, 2012
2) Thegeneticcodetellsuswhataminoacids
arerepresentedbythedifferentmRNA
codons.Thereare64differentcombinations
ofbases(codons),soseveraldifferent
codonscancodeforthesameaminoacid.
However,onecodoncannevercodefortwo
differentaminoacids.
Protein Synthesis Outline October 18, 2012
Protein Synthesis Outline October 18, 2012
D) Aminoacidsarelinkedtogetherintoa
chainastheyarebroughttotheribosome.
E) Proteinsynthesis
continuesuntilthe
ribosomereachesastop
codon,whichisa
codonthatsignalsthe
endoftheprotein.
Protein Synthesis Outline October 18, 2012