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Protein Synthesis Outline October 18, 2012 EQ I) Central Dogma of Biology: DNA transcription RNA translation Protein

Protein Synthesis Outline October 18, 2012

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Page 1: Protein Synthesis Outline October 18, 2012

Protein Synthesis Outline October 18, 2012

EQ

I) CentralDogmaofBiology:

DNA transcription RNA translation Protein

Page 2: Protein Synthesis Outline October 18, 2012

Protein Synthesis Outline October 18, 2012

Transcription

A) Tostarttranscription,manydifferent

proteinscometogetherandbindtoastrand

ofDNA.TheseproteinsincludeRNA

polymeraseandotherproteinsreferredto

astranscriptionfactors.Thesetranscription

factorsalsoincludehelicasetounwindthe

DNA.

Page 3: Protein Synthesis Outline October 18, 2012

Protein Synthesis Outline October 18, 2012

B) RNApolymeraseaddscomplementaryRNA

nucleotidestotheexposedDNAbases.

1) RNApolymeraserecognizesasequenceon

theDNAstrandcalledthepromoter

sequenceandbindstoit;thisisthestarting

pointfortranscription.

Page 4: Protein Synthesis Outline October 18, 2012

Protein Synthesis Outline October 18, 2012

2) RNApolymerasecanonlymoveinone

directiondowntheDNAstrand,soonlyone

sideoftheunwoundDNAisusedforthe

pairingofRNAnucleotides.Thisiscalledthe

templatestrand.

3) WhentheRNApolymerasereachesa

terminatorsequence,itstopstranscription

andthemRNAstrandisreleased.

Page 5: Protein Synthesis Outline October 18, 2012

Protein Synthesis Outline October 18, 2012

C) ThestrandofmRNAisreleasedfromthe

nucleus,passingthroughthenuclear

poresintothecytoplasm.

Page 6: Protein Synthesis Outline October 18, 2012

Protein Synthesis Outline October 18, 2012

III) Translation:“translating”themessage

fromthelanguageofnucleicacids

(nucleotides)tothelanguageofprotein

(aminoacids)

A) RibosomeattachestomRNA.

1) Ribosomesconsistof

twopieces,calledthe

largeandsmall

subunits.

2) Thesubunitsoftheribosome

surroundthemRNAstrand,muchlike

ahamburgerbunsurroundsthe

burger.

Page 7: Protein Synthesis Outline October 18, 2012

Protein Synthesis Outline October 18, 2012

B) Ribosomesreadcodonsoneatatime.

C) tRNAmoleculesbringaminoacidstothe

ribosome.

1) tRNAanticodonsarecomplementary

tothemRNAcodonsbeingreadbythe

ribosome.

Page 8: Protein Synthesis Outline October 18, 2012

Protein Synthesis Outline October 18, 2012

2) Thegeneticcodetellsuswhataminoacids

arerepresentedbythedifferentmRNA

codons.Thereare64differentcombinations

ofbases(codons),soseveraldifferent

codonscancodeforthesameaminoacid.

However,onecodoncannevercodefortwo

differentaminoacids.

Page 9: Protein Synthesis Outline October 18, 2012

Protein Synthesis Outline October 18, 2012

Page 10: Protein Synthesis Outline October 18, 2012

Protein Synthesis Outline October 18, 2012

D) Aminoacidsarelinkedtogetherintoa

chainastheyarebroughttotheribosome.

E) Proteinsynthesis

continuesuntilthe

ribosomereachesastop

codon,whichisa

codonthatsignalsthe

endoftheprotein.

Page 11: Protein Synthesis Outline October 18, 2012

Protein Synthesis Outline October 18, 2012