Protein Electrophoresis

By: Dr. Beenish Zaki

Introduction to Electrophoresis

Electrophoresis is a laboratory technique where the blood serum (is placed on agarose gel and exposed to an electrical current to separate the serum protein components.

Normal Pattern

                                     

                                                           

Note the relative sizes and shapes of the globulin fractions.

EQUIPMENT FOR ELECTROPHORESIS

ELECTROPHORETIC PACK

POWER PACK

AGAROSE GEL

Micropipette and Applicator

DRYER

INTERPRETATION OF ELECTROPHORESIS

Cirrhotic Pattern

In the cirrhotic pattern, the distinction between beta and gamma globulin is blurred and is sometimes referred to as the "beta-gamma bridge" pattern.

Monoclonal (M) Protein Present

Serum shows the M protein was IgG kappa.