POSTER PRESENTATION Open Access Protective role of Mannose binding Lectin (MBL2) promoter haplotypes on TB infection in South Indian HIV-1 patients Kalaimani Pandian 1 , Stalinraja Maruthamuthu 1 , Suresh Madasamy 2 , Jayalakshmi Mariakuttikan 1* From 2nd International Science Symposium on HIV and Infectious Diseases (HIV SCIENCE 2014) Chennai, India. 30 January - 1 February 2014 Background Mannose binding Lectin (MBL) mediates protection against infections by activating the complement system, but certain microorganisms may increase infectivity by exploiting this host defence system. Hence, the purpose of this study is to evaluate MBL genetic variants with the development of TB infection caused by Mycobacterium tuberculosis among HIV patients. Methods Blood samples from TB+ART+ and TB - ART+ (n=30) were collected. Genomic DNA was extracted from Periph- eral Blood Mononuclear Cells (PBMC) using salting out procedure. MBL promoter haplotypes of -550 H/L and -221Y/X associated with high, medium and low (HY, LY and LX) secretion was assessed by PCR-SSP. Results The Promoter haplotype (LY/LX) associated with defi- cient MBL levels conferred a protective role to TB in our study population with a significant difference (Chi-square (X 2 ) =4.00; p<0.05). Conclusion In this study, we could observe MBL2 promoter haplotypes with low MBL secretion may play a protective role to intracellular mycobacterium infections like TB in HIV seropositive individuals. Authors’ details 1 Department of Immunology, School of Biological Sciences, Madurai Kamaraj University, Madurai- 625021, India. 2 ART Centre, Govt. Theni Medical College and Hospital, Theni- 625512, India. Published: 27 May 2014 doi:10.1186/1471-2334-14-S3-P37 Cite this article as: Pandian et al.: Protective role of Mannose binding Lectin (MBL2) promoter haplotypes on TB infection in South Indian HIV-1 patients. BMC Infectious Diseases 2014 14(Suppl 3):P37. Submit your next manuscript to BioMed Central and take full advantage of: • Convenient online submission • Thorough peer review • No space constraints or color ﬁgure charges • Immediate publication on acceptance • Inclusion in PubMed, CAS, Scopus and Google Scholar • Research which is freely available for redistribution Submit your manuscript at www.biomedcentral.com/submit * Correspondence: [email protected] 1 Department of Immunology, School of Biological Sciences, Madurai Kamaraj University, Madurai- 625021, India Full list of author information is available at the end of the article Pandian et al. BMC Infectious Diseases 2014, 14(Suppl 3):P37 http://www.biomedcentral.com/1471-2334/14/S3/P37 © 2014 Pandian et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Protective role of Mannose binding Lectin (MBL2) promoter haplotypes on TB infection in South Indian HIV-1 patients

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Page 1: Protective role of Mannose binding Lectin (MBL2) promoter haplotypes on TB infection in South Indian HIV-1 patients

POSTER PRESENTATION Open Access

Protective role of Mannose binding Lectin (MBL2)promoter haplotypes on TB infection in SouthIndian HIV-1 patientsKalaimani Pandian1, Stalinraja Maruthamuthu1, Suresh Madasamy2, Jayalakshmi Mariakuttikan1*

From 2nd International Science Symposium on HIV and Infectious Diseases (HIV SCIENCE 2014)Chennai, India. 30 January - 1 February 2014

BackgroundMannose binding Lectin (MBL) mediates protectionagainst infections by activating the complement system,but certain microorganisms may increase infectivity byexploiting this host defence system. Hence, the purposeof this study is to evaluate MBL genetic variants with thedevelopment of TB infection caused by Mycobacteriumtuberculosis among HIV patients.

MethodsBlood samples from TB+ART+ and TB-ART+ (n=30)were collected. Genomic DNA was extracted from Periph-eral Blood Mononuclear Cells (PBMC) using salting outprocedure. MBL promoter haplotypes of -550 H/L and-221Y/X associated with high, medium and low (HY, LYand LX) secretion was assessed by PCR-SSP.

ResultsThe Promoter haplotype (LY/LX) associated with defi-cient MBL levels conferred a protective role to TB in ourstudy population with a significant difference (Chi-square(X2) =4.00; p<0.05).

ConclusionIn this study, we could observe MBL2 promoter haplo-types with low MBL secretion may play a protective roleto intracellular mycobacterium infections like TB in HIVseropositive individuals.

Authors’ details1Department of Immunology, School of Biological Sciences, Madurai KamarajUniversity, Madurai- 625021, India. 2ART Centre, Govt. Theni Medical Collegeand Hospital, Theni- 625512, India.

Published: 27 May 2014

doi:10.1186/1471-2334-14-S3-P37Cite this article as: Pandian et al.: Protective role of Mannose bindingLectin (MBL2) promoter haplotypes on TB infection in South IndianHIV-1 patients. BMC Infectious Diseases 2014 14(Suppl 3):P37.

Submit your next manuscript to BioMed Centraland take full advantage of:

• Convenient online submission

• Thorough peer review

• No space constraints or color figure charges

• Immediate publication on acceptance

• Inclusion in PubMed, CAS, Scopus and Google Scholar

• Research which is freely available for redistribution

Submit your manuscript at www.biomedcentral.com/submit

* Correspondence: [email protected] of Immunology, School of Biological Sciences, Madurai KamarajUniversity, Madurai- 625021, IndiaFull list of author information is available at the end of the article

Pandian et al. BMC Infectious Diseases 2014, 14(Suppl 3):P37http://www.biomedcentral.com/1471-2334/14/S3/P37

© 2014 Pandian et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative CommonsAttribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction inany medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

mailto:[email protected]

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