PromoCellKatalog2013_lowres

8/10/2019 PromoCellKatalog2013_lowres

1/212


2/212


3/212

1

Contents

Overview

Human Primary Cells1

3 Media and Sera

4 Cell Pellets

5 Reagents and Supplements

6

Overview

Microbial Detection and Elimination

7

Human Stem and Blood Cells2

Cell Analysis

Apoptosis

Fluorescent Labeling

Cell Transfection

Antibodies and ELISAs

Cytokines and Growth Factors

8

9

10

11

12

About PromoCell0


4/212

Contents2

About PromoCellPromoCell Ethical Standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6Introduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

PromoCells Knowledge Base. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Human Primary Cells1.1 Cardiac Myocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

1.2 Chondrocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

1.3 Endothelial Cells (Large Vessels) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

1.4 Endothelial Cells (Microvascular) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

1.5 Epithelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

1.6 Fibroblasts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 271.7 Follicle Dermal Papilla Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

1.8 Hepatocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

1.9 Keratinocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

1.10 Melanocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

1.11 Osteoblasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

1.12 Preadipocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

1.13 Skeletal Muscle Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

1.14 Smooth Muscle Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

Overview Human Primary Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

Human Primary Cells by Tissue Type . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

Human Stem and Blood Cells2.1 Mesenchymal Stem Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

2.2 Pericytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

2.3 CD34+Progenitor Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

2.4 CD133+ Progenitor Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

2.5 Monocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

2.6 Mononuclear Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

Overview Human Stem and Blood Cells. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

Media and Sera3.1 Media for Primary Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

3.2 Media for Stem and Blood Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

3.3 Classical Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

3.4 Insect Cell Culture Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87

3.5 Sera. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87

Cell Pellets4.1 Cell Pellets. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91

1

2

Contents

3

4

0


5/212


6/212

Contents4

Contents

Antibodies and ELISAs10.1 Mono- and Polyclonal Antibodies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 169

10.2 Antigens and Cell Lysates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 172

10.3 Labeled Antibodies and Labeling Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 173

10.4 ELISA Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 175

Cytokines and Growth Factors11.1 Cytokines, Growth Factors, Hormones, and soluble Receptors . . . . . . . . . . 179

11.2 Animal-free Cytokines and Growth Factors . . . . . . . . . . . . . . . . . . . . . . . . 180

11.3 Cytokines and Growth Factors for Stem Cell Research. . . . . . . . . . . . . . . . 181

Microbial Detection and Elimination12.1 Mycoplasma Test Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 185

12.2 Mycoplasma Elimination Solutions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 186

12.3 Bacteria Test Kit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 188

12.4 Disinfectants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 189

Appendix

Alphabetical Index. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 191International Distributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 200

Terms and Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 203

Ordering and Technical Support. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 205

Fax Order Form. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 209

12

11

13

10


7/212

Over 20 years ago PromoCell started its business in the

so-called City of Science: Heidelberg. Heidelberg is not only

home to the oldest university in Germany but is also popular for

research related businesses. The city provides the ideal scientific

environment for PromoCell and serves as initial point for all our

operations.

About PromoCell 0


8/212

6 0 About PromoCell

PromoCell Ethical Standards

Ethical and legal standards

governing Primary Human Cells

PromoCell is the original manufacturer

of all primary, stem, and blood cells fea-tured in this catalog and is committed to

1. The Convention for Protectionof Human Rights and Dignity of the

Human Being with Regard to the Ap-

plication of Biology and Medicine:

Convention of Human Rights and

Biomedicine published on April 4th,

1997 by the Council of Europe (Euro-

pean Treaty Series no 164).

2. The Human Tissue Act published

on November 15th, 2004 by the gov-

ernment of the United Kingdom. This

act aims to make consent a funda-mental principle underpinning the use

and storage of human tissue.

Should you have any questions con-

cerning ethical aspects related to the

use of Primary Human Cells, do not

hesitate to contact us or visit our

website:

www.promocell.com/ethics

Extract from The Convention of Human Rights and Biomedicine:

Article 5 General ruleAn intervention in the health field may only be carried out after the person

concerned has given free and informed consent to it.

This person shall beforehand be given appropriate information as to the pur-

pose and nature of the intervention as well as on its consequences and risks.

The person concerned may freely withdraw consent at any time.

Article 22 Disposal of a removed part of the human body

When in the course of an intervention any part of a human body is removed,

it may be stored and used for a purpose other than that for which it was

removed, only if this is done in conformity with appropriate information and

consent procedures.

Extract from The Declaration of Helsinki:

Article 22

In any research on human beings, each potential subject must be adequately

informed of the aims, methods, sources of funding, any possible conflicts of

interest, institutional affiliations of the researcher, the anticipated benefits

and potential risks of the study and the discomfort it may entail. The subject

should be informed of the right to abstain from participation in the study or

to withdraw consent to participate at any time without reprisal. After ensuring

that the subject has understood the information, the physician should thenobtain the subjects freely-given informed consent, preferably in writing. If

the consent cannot be obtained in writing, the non-written consent must be

formally documented and witnessed.

The tissue used by PromoCell for the

isolation of human cell cultures is de-

rived from donors who have signed

an informed consent form (this being

done by the donor himself, an autho-rized agent, or a legal agent) which

outlines in detail the purpose of the

donation and the procedure for pro-

cessing the tissue. We do not accept

or use any tissue without prior signing

of the consent documents.

When obtaining and using human

tissue, PromoCell acts in strict compli-

ance with

the highest ethical and legal standards.

Consequently, PromoCell strictly com-

plies with the following procedure for

the donation and collection of human

tissue used for cell isolation:

3. The Declaration of Helsinki de-

veloped by the World Medical Associ-

ation as a statement of ethical princi-

ples to provide guidance to physicians

and other participants in medical

research involving human subjects.

4. The German Federal Data Protec-

tion Act to protect privacy of donors.


9/212

7

AboutPromoCell

0 About PromoCell

Dear Customer,

Henry Ford once said: Coming together is a beginning. Keepingtogether is progress.Working together is success. At PromoCellwe are proud to work together successfully with scientists world-wide, providing them with high quality products and experiencedtechnical customer support. With over 5,000 PromoCell andPromoKine products we have the tools to meet the needs of yourresearch project!

Under our PromoCell brand we offer a broad range of humanprimary cells, stem cells and blood cells, as well as optimized cellculture media. Scientists worldwide use PromoCell products in ba-sic and applied biomedical research to obtain better results frommore accurate physiological models. The reliability of our cells pro-vides the basis for being able to offer you the high quality stan-dards you need.

For more in depth analysis of your cells in culture, why not look toour PromoKinebrand? PromoKine offers quality kits and reagentsfor cell biology including those for cell analysis, cell transfection,

and fluorescent labeling and also provides numerous antibodies,ELISAs, cytokines and growth factors.

In order to complete our portfolio we established the PromoCellAcademy in 2004. The PromoCell Academy offers courses withstate-of-the-art insight and up to date trends in the Life Sciencesin a professional and hands-on setting. You can choose from agrowing collection of course topics in German as well as in English.

We are looking forward to working with you and providing youwith our support!

Scientists supporting Scientists guaranteed.

Your PromoCell Team

At PromoCell we continuously work to improve our products and services.


10/212


11/212


12/212

Content Chapter 110

Human Primary Cells

1.1 Cardiac Myocytes . . . . . . . . . . . . . . . . . . . 11

1.2 Chondrocytes . . . . . . . . . . . . . . . . . . . . . . 12

1.3 Endothelial Cells (Large Vessels) . . . . . . . . 13

Umbilical Vein . . . . . . . . . . . . . . . . . . . . . . 13

Umbilical Artery. . . . . . . . . . . . . . . . . . . . . 14

Aortic / Coronary Artery . . . . . . . . . . . . . . 15

Pulmonary Artery / Saphenous Vein . . . . . 16

1.4 Endothelial Cells (Microvascular) . . . . . . . . 17

Dermal . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 Dermal Blood . . . . . . . . . . . . . . . . . . . . . . 18

Dermal Lymphatic . . . . . . . . . . . . . . . . . . . 19

Bladder / Cardiac. . . . . . . . . . . . . . . . . . . . 20

Pulmonary / Uterine . . . . . . . . . . . . . . . . . 21

Brain . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

1.5 Epithelial Cells . . . . . . . . . . . . . . . . . . . . . . 23

Nasal / Tracheal. . . . . . . . . . . . . . . . . . . . . 23

Bronchial / Small Airway . . . . . . . . . . . . . . 24

Mammary / Renal . . . . . . . . . . . . . . . . . . . 25

Renal Cortical / Placental. . . . . . . . . . . . . . 26

1.6 Fibroblasts . . . . . . . . . . . . . . . . . . . . . . . . . 27

Dermal . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Pulmonary / Aortic Adventitial / Cardiac . . 28

Uterine / Villous Mesenchymal . . . . . . . . . 29

1.7 Follicle Dermal Papilla Cells . . . . . . . . . . . . 30

1.8 Hepatocytes . . . . . . . . . . . . . . . . . . . . . . . 31

1.9 Keratinocytes. . . . . . . . . . . . . . . . . . . . . . . 32

1.10 Melanocytes . . . . . . . . . . . . . . . . . . . . . . . 33

1.11 Osteoblasts . . . . . . . . . . . . . . . . . . . . . . . . 34

1.12 Preadipocytes . . . . . . . . . . . . . . . . . . . . . . 35

1.13 Skeletal Muscle Cells . . . . . . . . . . . . . . . . . 36

1.14 Smooth Muscle Cells . . . . . . . . . . . . . . . . . 37

Aortic . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

Coronary Artery / Pulmonary Artery . . . . . 38

Umbilical Artery / Tracheal . . . . . . . . . . . . 39

Bronchial / Bladder . . . . . . . . . . . . . . . . . . 40

Prostate / Uterine . . . . . . . . . . . . . . . . . . . 41

Overview Human Primary Cells . . . . . . . . . . . . . . 42

Human Primary Cells by Tissue Type . . . . . . . . . . 45

1


13/212

1 Human Primary Cells 11

HumanPrimary

Cells

1.1 Cardiac Myocytes

Fig. 1: HCM culture in phase contrast.

Fig. 2: Flow cytometric analysis of PromoCell HCM

after being cultured for 60 days under confluent condi-

tions. 96 % of the cells express sarcomeric -actinin, a

marker of late differentiation. CellLabQuantaSCTM,

BeckmanCoulter,Inc.

0

Sarcomeric -actinin

10 102 103 104

Count

Sarcomeric

-actinin negative

50

0

25

75 Sarcomeric

-actinin positive

PromoCell offers a wide variety of high

quality human primary cells. The tissue

used for the isolation of these cells is

obtained from approved medical cen-

ters following strict ethical standards

(see page 6 for details). Shortly afterisolation, the cells are cryopreserved

using the unique serum-free PromoCell

Cryo-SFM (see page 79) and an op-

timized, computer controlled freezing

profile. This allows high viability of the

cells and defined culture conditions after

thawing.

All PromoCell primary cells are available

cryopreserved or proliferating. Each celllot is tested for cell type specific markers,

cell morphology, population doubling

time, and proliferation capacity. In addi-

tion, tests for the absence of HIV 1, HIV 2,

HBV, HCV, fungi, mycoplasma, and other

bacteria are performed.

Moreover, cell pellets prepared from re-

leased cell lots and stored in RNAlater

are available. They are useful for DNA,RNA, and protein isolation and their sub-

sequent analysis (for more information

see page 91).

Related products

Human Cardiac Microvascular

Endothelial Cells (see page 20)

Human Cardiac Fibroblasts

(see page 28)

HCM Pellet (see page 91)

PromoFectin Transfection Reagent

(see page 157)

Human Primary Cells

https://www.promocell.com/shop

Applications

Cardiac research

Physiological studies

Pharmacological studies

Features

Adult origin

Sarcomeric -actinin positive

Slow muscle myosin positive

Qualified for long-term experiments

Human Cardiac Myocytes (HCM) are

isolated from the ventricles of the adult

heart. They are qualified for in vitro

research on cardiac diseases and for

pharmacological studies. Unlike freshlyisolated rod-shaped myocytes, cultured

HCM can be used for long-term experi-

ments like investigating the long-term

effects of cytokines, mechanical strain,

or cell-cell interactions, as they are pre-

pared according to a special protocol.

Initially, the HCM act more like progeni-

tor cells in that they are not yet fully dif-

ferentiated. They express the markersof early stage differentiation such as

GATA-4 and sarcomeric alpha-actin and

have a high capacity for proliferation.

When they are grown to confluency

and cultivated for an extended period of

time, the differentiation process begins.

Markers of late differentiation (e.g. sar-

comeric alpha-actinin, slow muscle myo-

sin) are increased and the cells begin to

form myotube-like structures.

Recommended media & reagents Myocyte Growth Medium (see page 62)

DetachKit (see page 95)

Cryo-SFM (see page 79)

Product Size Catalog Number

Human Cardiac Myocytes (HCM) 500,000 cryopreserved cells500,000 proliferating cells

C-12810C-12811

HCM Pellet 1x106cells in RNAlater C-14080


14/212

1 Human Primary Cells12

Cells

1.2 Chondrocytes

Applications

Osteoarthritis research

Joint research

Physiology studies Chondrocyte differentiation

experiments

Hyaline cartilage investigations

Features

Available from hip and knee joints

Differentiation tested by Alcian Blue

staining of HCH spheroids

Cryopreserved at second passage

Human Chondrocytes (HCH) are isolat-ed from normal human articular cartilage

from the knee and hip joints (lot specific

source information is available on re-

quest). PromoCell HCH are routinely

tested for their capacity to differentiate

in 3D spheroids (see Fig. 2).

The articular cartilage covers the joints

between bones and contains no blood

vessels, lymphatic vessels, or nerve fi-

bers. It is composed of only one special-

ized cell type, the chondrocytes, which

produce and maintain the extracellular

matrix of cartilage, i.e. collagen (mostly

type II) and proteoglycans (primarily ag-

grecan).


Human Chondrocytes (HCH) 500,000 cryopreserved cells500,000 proliferating cells

C-12710C-12750

HCH Pellet 1x106cells in RNAlater C-14070

Fig. 1: HCH culture in phase contrast.

Fig. 2: Spheroids of differentiated HCH

cultured in Chondrocyte Growth Medium

and stained with Alcian Blue.

Recommended media & reagents

Chondrocyte Growth Medium

(see page 62)



Related products

HCH Pellet (see page 91)


(see page 157)



15/212


16/212


Cells

Fig. 4: HUVEC culture in phase contrast.


Human Umbilical Artery Endothelial Cells(HUAEC)

500,000 cryopreserved cells500,000 proliferating cells

C-12202C-12252

HUAEC Pellet 1x106cells in RNAlater C-14013

Human Umbilical Artery

Endothelial Cells (HUAEC)

HUAEC are isolated from the arteries ofthe umbilical cord and are often used for

in vitro studies on atherosclerosis but

also for endothelial cell-dependent lym-

phocyte activation and chemoattractant

activity experiments*.


Endothelial Cell Growth Medium

(see page 63)

Endothelial Cell Growth Medium 2(see page 63)



Related products

HUAEC Pellet (see page 92)

Human Umbilical Vein Endothelial

Cells (see page 13) Human Umbilical Artery Smooth

Muscle Cells (see page 39)

PromoFectin-HUVEC Transfection

Reagent (see page 158)

CD31 Antibody

(see page 169)

*Human Umbilical Vein Endothelial Cells (HUVEC) from the same donor are available on request.



Human Umbilical Vein Endothelial Cells(HUVEC) single donor


C-12200C-12250

HUVEC single donor Pellet 1x106cells in RNAlater C-14010

Human Umbilical Vein Endothelial Cells(HUVEC) pooled


C-12203C-12253

HUVEC pooled Pellet 1x106cells in RNAlater C-14011

Human Umbilical Vein Endothelial Cells(HUVEC) isolated in Growth Medium 2,single donor


C-12206C-12207

HUVEC Growth Medium 2 single donorPellet

1x106cells in RNAlater C-14008

Human Umbilical Vein Endothelial Cells(HUVEC) isolated in Growth Medium 2,pooled


C-12208C-12209

HUVEC Growth Medium 2 pooled Pellet 1x106cells in RNAlater C-14009

Human Umbilical Vein Endothelial Cells(HUVEC) pre-screened


C-12205C-12255

HUVEC pre-screened Pellet 1x106cells in RNAlater C-14012


Related products

HUVEC single donor Pellet

(see page 92)

HUVEC pooled Pellet (see page 92)

HUVEC Growth Medium 2 single

donor Pellet (see page 92) HUVEC Growth Medium 2 pooled

Pellet (see page 92)

HUVEC pre-screened Pellet

(see page 92)

Human Umbilical Artery Endothelial

Cells (see below)



VEGF(see page 179)

CD31 and VEGF Antibodies(see page 169)


17/212


HumanPrimary

Cells

Human Coronary Artery

Endothelial Cells (HCAEC)

HCAEC are isolated from the coronary

arteries (the right and the left coronary

artery, including the anterior descend-

ing and the circumflex branches) from a


Human Coronary Artery Endothelial Cells(HCAEC)


C-12221C-12222

HCAEC Pellet 1x106cells in RNAlater C-14022


Human Aortic Endothelial Cells (HAoEC) 500,000 cryopreserved cells500,000 proliferating cells

C-12271C-12272

HAoEC Pellet 1x10

6

cells in RNAlater

C-14023

Human Aortic Endothelial Cells

(HAoEC)

HAoEC are isolated from the human

ascending (thoracic) and descend-

ing (abdominal) aorta. They are usefulfor studying vascular diseases such as

thrombosis, atherosclerosis, and hyper-

tension as well as for stent-graft compat-

ibility testing.


Endothelial Cell Growth Medium MV

(see page 64)

Endothelial Cell Growth Medium MV2

(see page 64)

DetachKit (see page 95) Cryo-SFM (see page 79)

Fig. 5: Location of the coronary arteries.

Right coronary artery

Left anterior descending

coronary artery

Circumflex coronary

artery

Left main coronary

artery

Related products

HAoEC Pellet (see page 91)

Human Aortic Smooth Muscle Cells

(see page 37)

Human Aortic Adventitial Fibroblasts

(see page 28) PromoFectin-HUVEC Transfection


CD31 Antibody

(see page 169)

single donor. They are crucially involved

in the regulation of coronary blood flow

and cardiac functions and are conse-

quently useful for in vitro studies of

diseases such as thrombosis, atheroscle-

rosis, and hypertension. HCAEC are also

suitable for studying functional vaso-

dilators, such as prostacyclin, bradykinin,

or nitric oxide.



(see page 64)


(see page 64)


Related products

HCAEC Pellet (see page 91)

Human Coronary Artery Smooth




CD31 Antibody

(see page 169)




18/212


Cells


Human Pulmonary Artery Endothelial Cells(HPAEC) 500,000 cryopreserved cells500,000 proliferating cells C-12241C-12242

HPAEC Pellet 1x106cells in RNAlater C-14024

Human Pulmonary Artery

Endothelial Cells (HPAEC)

HPAEC are isolated from human pul-

monary arteries (the main pulmonaryartery, left and right branches). Since the

pulmonary arteries carry blood from the

heart to the lungs, they are the only ar-

teries (other than umbilical arteries) that

carry deoxygenated blood.



(see page 63)

Endothelial Cell Growth Medium 2

(see page 63) DetachKit (see page 95)



Human Saphenous Vein Endothelial Cells(HSaVEC)


C-12231C-12232

HSaVEC Pellet 1x106

cells in RNAlater

C-14025

Human Saphenous Vein

Endothelial Cells (HSaVEC)

HSaVEC are isolated from human saphe-

nous veins from single donors. The

saphenous vein is often used for auto-transplantation in coronary artery bypass

operations, when arterial grafts are not

available or many grafts are required.



(see page 63)

Endothelial Cell Growth Medium 2

(see page 63)


Related products

HPAEC Pellet (see page 92)

Human Pulmonary Artery Smooth


PromoFectin-HUVEC TransfectionReagent (see page 158)

CD31 Antibody

(see page 169)

Related products

HSaVEC Pellet (see page 92)



CD31 Antibody

(see page 169)




19/212

1.4 Endothelial Cells (Microvascular)

Applications

Angiogenesis research

Oncology

Drug uptake studies Cell-cell junction investigation

Inflammation studies

Features

Von Willebrand factor (vWF) positive

CD31 positive

Smooth muscle -actin negative


The walls of capillaries are composed of a

single layer of microvascular endothelialcells. This layer is so thin that molecules

such as oxygen, water, and lipids can pass

through it by diffusion and enter the sur-

rounding tissues. Waste products such

as carbon dioxide and urea, on the other

hand, diffuse back into the blood to be

carried away. Because of their location

at the interface between the circulating

fluid in the lumen and the surrounding

tissue, microvascular endothelial cells are

highly active and closely involved in nu-

merous physiological processes.

Microvascular endothelial cells differ in

morphologies and properties according

to the tissues supplied by the capillaries.

Therefore, PromoCell microvascular en-

dothelial cells are available from dermal,

lung, cardiac, uterine, bladder, and brain

tissues.

Available endothelial cell types (micro-

vascular):

HDMEC (see below) HDBEC (see page 18)

HDLEC (see page 19)

HBdMEC (see page 20)

HCMEC (see page 20)

HPMEC (see page 21)

HUtMEC (see page 21)

HBMEC (see page 22)


Human Dermal Microvascular

Endothelial Cells (HDMEC)

HDMEC are isolated from the dermis of

juvenile foreskin and adult skin (differ-

ent locations). Since the dermis contains

blood and lymphatic capillaries, HDMEC

comprise Blood and Lymphatic Micro-

vascular Endothelial Cells. Both have a

common origin and can be identified by

several markers.

In addition, HDMEC are available which

are pre-screened for VEGF (vascular en-

dothelial growth factor) response. VEGF

is an important signaling protein involvedin both vasculogenesis and angiogenesis.

In vitro, VEGF has been shown to stimu-

late endothelial cell mitogenesis, cell

migration, sprouting, and microvascular

permeability.



(see page 64)


(see page 64)



Related products

HDMEC Pellet (see page 91)

HDMEC adult Pellet (see page 91) HDMEC pre-screened Pellet

(see page 91)

Human Pericytes (see page 51)


Reagent (see page 158) VEGF (see page 179)

CD31 Antibody

(see page 169)

Fig. 2: HDMEC culture stained for CD31

antigen. Nuclei counterstained with DAPI.

Fig. 3: HDMEC culture in phase contrast.

Fig. 1: HDMEC culture stained for vWF

(Factor VIII antigen). Nuclei counter-

stained with DAPI.


HumanPrimary

Cells

Human Dermal Microvascular EndothelialCells (HDMEC) juvenile foreskin


C-12210C-12260

HDMEC Pellet 1x106cells in RNAlater C-14015

Human Dermal Microvascular EndothelialCells (HDMEC) adult donor


C-12212C-12262

HDMEC adult Pellet 1x106cells in RNAlater C-14016

Human Dermal Microvascular EndothelialCells (HDMEC) pre-screened


C-12215C-12265

HDMEC pre-screened Pellet 1x106cells in RNAlater C-14017



20/212

Human Dermal Blood Endothelial

Cells (HDBEC)

HDBEC are isolated from the dermis of

juvenile foreskin and adult skin (different

locations) from a single donor*.The cells are routinely analyzed by flow

cytometric analysis and immunofluo-

rescent staining: they are > 95% CD31

positive (see Fig. 4) and stain positive for

von Willebrand factor. In addition, they

are > 90% negative for podoplanin

(see Fig. 5) and stain negative for

smooth muscle specific -actin.

Blood Endothelial Cells have a key func-

tion in physiological processes like vessel

tonus, capillary permeability, blood co-

agulation, fibrolysis, and angiogenesis.

They are also useful for disease studies

of atherosclerosis, tumorigenesis, andthrombosis.



(see page 64)




Human Dermal Blood Endothelial Cells(HDBEC) juvenile foreskin


C-12211C-12214

HDBEC Pellet 1x106cells in RNAlater C-14018

Human Dermal Blood Endothelial Cells(HDBEC) adult donor


C-12225C-12226

HDBEC adult Pellet 1x106cells in RNAlater C-14019

*Human Dermal Lymphatic Microvascular Endothelial Cells (HDLEC) from the same donor are available on request.


Related products

HDBEC Pellet (see page 91)

HDBEC adult Pellet (see page 91)

Human Dermal Lymphatic Endothelial

Cells (see page 19)

PromoFectin-HUVEC TransfectionReagent (see page 158)

Podoplanin, CD31, and Prox-1

Antibodies (see page 169)


Cells

Fig. 4: Flow cytometric analysis of PromoCell HDBEC.

98% of the cells are CD31 positive. CellLabQuantaSCTM,

BeckmanCoulter,Inc.

0

CD31

10 102 103 104

Count

50

0

25

75

CD31 negative CD31 positive

Fig. 5: Flow cytometric analysis of PromoCell HDBEC.

91% of the cells are podoplanin negative. CellLabQuantaSCTM,

BeckmanCoulter,Inc.

0

Podoplanin

10 102 103 104

Count

50

0

25

75

Podoplanin negative Podoplanin positive


21/212



Human Dermal Lymphatic EndothelialCells (HDLEC) juvenile foreskin


C-12216C-12218

HDLEC Pellet 1x106cells in RNAlater C-14020

Human Dermal Lymphatic EndothelialCells (HDLEC) adult donor


C-12217C-12219

HDLEC adult Pellet 1x106cells in RNAlater C-14021


HumanPrimary

Cells

Human Dermal Lymphatic

Endothelial Cells (HDLEC)

HDLEC are isolated from the dermis of

juvenile foreskin and adult skin (different

locations) from a single donor*. The cells

are routinely analyzed by flow cytometricanalysis and immunofluorescent staining:

> 95% of the cells are CD31 positive and

podoplanin positive (see Fig. 6 and 7).

In addition, the cells stain positive for

Prox-1.

Lymphatic vessels transport excess fluids

from tissues to the circulatory system and

are a major component of the immune

system. The transported fluid is nearly

cell free. The vessels are highly perme-

able, because they lack a continuous

basal membrane. Lymphatic endothelial

cells are involved in pathological altera-

tions of the lymphatic system. During

tumor lymphangiogenesis, the lymphaticendothelial cells build new vessels that

infiltrate tumors, attract tumor cells, and

induce tumor cell metastasis.



(see page 64)

*Human Dermal Blood Endothelial Cells (HDBEC) from the same donor are available on request.

Fig. 6: Flow cytometric analysis of PromoCell HDLEC.

99% of the cells are CD31 positive.

CD31

CD31 negative CD31 positive

CellLabQuantaSCTM,

BeckmanCoulter,Inc.

0 10 102 103 104

Count

50

0

25

75

Podoplanin

Podoplanin negative Podoplanin positive

Fig. 7: Flow cytometric analysis of PromoCell HDLEC.

96% of the cells are podoplanin positive. CellLabQuanta

SCTM,

BeckmanCoulter,Inc.

0 10 102 103 104

Count

50

0

25

75



Related products

HDLEC Pellet (see page 91)

HDLEC adult Pellet (see page 91)

Human Dermal Blood EndothelialCells (see page 18)



VEGF (see page 179)

Podoplanin, CD31, and Prox-1

Antibodies (see page 169)


22/212




Cells

Human Bladder Microvascular

Endothelial Cells (HBdMEC)

HBdMEC are isolated from the human

bladder microvasculature. The cells areroutinely analyzed by immunofluores-

cent staining: they stain positive for

CD31 and von Willebrand factor and

negative for smooth muscle -actin.

HBdMEC are useful in studying the

role of microvascular endothelial

cells in the control of inflammation,

capillary exchange, tissue growth, and

angiogenesis.



(see page 64)




Related products

HBdMECPellet (see page 91)



CD31 Antibody(see page 169)


Human Bladder Microvascular EndothelialCells (HBdMEC)


C-12291C-12292

HBdMEC Pellet 1x106cells in RNAlater C-14026


Human Cardiac MicrovascularEndothelial Cells (HCMEC)


C-12285C-12286

HCMEC Pellet 1x106cells in RNAlater C-14029

Related products

HCMEC Pellet (see page 91) Human Cardiac Myocytes (see page 11)

Human Cardiac Fibroblasts

(see page 28)



CD31 Antibody

(see page 169)


Endothelial Cells (HCMEC)

HCMEC are isolated from heart ven-

tricles from single donors. The cells are

routinely analyzed by immunofluores-

cent staining: they stain positive for



HCMEC intensively interact with cardio-

myocytes and therefore have a charac-

teristic phenotype different from other

microvascular endothelial cells. HCMEC

play an important role in the physiologi-

cal regulation of coronary blood flowand capillary exchange. Medical condi-

tions such as hypertrophy, ischemia,

hypertension, and diabetes mellitus are

associated with endothelial dysfunction.



(see page 64)


(see page 64)




23/212



HumanPrimary

Cells


Human Pulmonary Microvascular

Endothelial Cells (HPMEC)

HPMEC are isolated from the lung from

a single donor. The cells are routinelyanalyzed by immunofluorescent stain-

ing: they stain positive for CD31 and

von Willebrand factor and negative for

smooth muscle -actin.

The lung has a vast endothelial surface

area, which is essential for the exchange

of gases. HPMEC are most appropriate

for studying human lung diseases.



(see page 64)




Related products

HPMEC Pellet (see page 92)

Human Pulmonary Fibroblasts

(see page 28)

Human Small Airway Epithelial Cells(see page 24)



CD31 Antibody

(see page 169)


Human Pulmonary MicrovascularEndothelial Cells (HPMEC)


C-12281C-12282

HPMEC Pellet 1x106cells in RNAlater C-14027

Human Uterine Microvascular

Endothelial Cells (HUtMEC)

HUtMEC are isolated from the myome-

trium (middle layer of the uterine wall)

from a single donor, who has not been

pre-treated with hormones. The cells

are routinely analyzed by immunofluo-

rescent staining: they stain positive for



During pregnancy HUtMEC propagate

intensely and change their phenotype

and expression pattern in response to

sex steroids. This includes up-regulation

of HUtMEC nitric oxide synthase activ-

ity, modulation of adhesion molecule

expression, and an increase in endothe-lial cell survival and angiogenic activity.

HUtMEC are involved in medical condi-

tions such as uterine fibroma or adeno-

myosis.



(see page 64)


(see page 64)



Related products

HUtMEC Pellet (see page 92)

Human Uterine Fibroblasts(see page 29)

Human Uterine Smooth Muscle Cells

(see page 41)



CD31 Antibody

(see page 169)


Human Uterine MicrovascularEndothelial Cells (HUtMEC)


C-12295C-12296

HUtMEC Pellet 1x106cells in RNAlater C-14028


24/212



Cells


Human Brain Microvascular EndothelialCells (HBMEC)


C-12287C-12288

HBMEC Pellet 1x106cells in RNAlater C-14032

Human Brain Microvascular

Endothelial Cells (HBMEC)

Human Brain Microvascular Endothelial

Cells (HBMEC) are isolated from corticaltissue (see Fig. 8) from a single donor.

They are routinely analyzed by flow

cytometry and immunofluorescent stai-

ning. The cells are positive for CD31 and

von Willebrand factor and negative for

smooth muscle -actin and CD90.

Brain microvascular endothelial cells are

highly specialized cells responsible for

the formation and functional properties

of the Blood-Brain Barrier (BBB). The

BBB is characterized by cellular tightjunctions and active transport systems

which allow the selective transport of

substances into the brain, and prevent

the entrance of unwanted substances.

Therefore, PromoCell HBMEC are well

suited for studying brain endothelial cell

functions like active transport systems

and drug uptake. Furthermore, neuro-

inflammation studies as well as tight

junction research can be performed

with HBMEC.



(see page 64)



Fig. 9: HBMEC in phase contrast.Fig. 8: Schematic structure of the brain.

Cerebral cortex

Cerebellum

Spinal cord

Pituitary gland

Hypothalamus

Corpus callosum

Thalamus

Limited Availability




25/212


HumanPrimary

Cells

1.5 Epithelial Cells

Applications

Drug uptake studies

Toxicological research

Wound healing researchOncology

Cell differentiation studies

Respiratory disease research

Features

Cytokeratin positive

Available from different tissues


The epithelium is the interface between the

body and the external environment. It cov-ers all outside surfaces and inside lumina

e.g. the inner lining of the respiratory

tract. In addition, epithelial cells also make

up exocrine and endocrine glands. The

functions of epithelial cells are diverse and

include absorption, secretion, protection,

transcellular transport, and sensation.

Fig. 1: HTEpC culture in phase contrast.

Note the cuboidal shape typical for air-

way epithelial cells.

Fig. 2: HTEpC culture stained for cy-

tokeratin. Nuclei counterstained with

DAPI.

Typically, epithelial tissue is made of

tightly packed cells with little intercel-

lular space and is arranged in flat sheets

forming effective barriers between the

underlying tissues and the external en-vironment.

PromoCell offers epithelial cells from dif-

ferent locations:

Airways: Nasal mucosa, trachea, bronchi,

and distal respiratory tract

Breast: Mammary gland

Kidney: Whole kidney and renal cortex

Placenta: Amniotic membrane

Available Epithelial Cells:

HNEpC (see below) HTEpC (see below)

HBEpC (see page 24)

HSAEpC (see page 24)

HMEpC (see page 25)

HREpC (see page 25)

HRCEpC (see page 26)

HPlEpC (see page 26)

Human Nasal Epithelial Cells

(HNEpC)

HNEpC are isolated from normal human

nasal mucosa and stain positive for cy-

tokeratin.

The epithelium in the nasal cavity cleans,

humidifies, and warms inhaled air. In ad-

dition, it produces mucus, which binds

particles that are subsequently trans-

ported to the pharynx by cilia on the

epithelial cells. HNEpC are useful for invitrostudies of these processes.


Airway Epithelial Cell Growth Medium

(see page 65)


Human Nasal Epithelial Cells (HNEpC) 500,000 cryopreserved cells500,000 proliferating cells

C-12620C-12621

HNEpC Pellet 1x106cells in RNAlater C-14062

Human Tracheal Epithelial Cells

(HTEpC)

HTEpC are isolated from the surface

epithelium of human trachea and stain

positive for cytokeratin.

The respiratory epithelia are respon-

sible for the lubrication of the lungs,

the maintenance of humidity, and the

cleaning of the respiratory tract. They

are an important target for drugs, tox-

ins, and carcinogens. In addition, they

are involved in many diseases such as

cystic fibrosis. Consequently, HTEpC are

useful for investigating the function and

pathology of the respiratory system.



(see page 65)



Related products

HNEpC Pellet (see page 92)


(see page 157)



Related products

HTEpC Pellet (see page 92)

Human Tracheal Smooth Muscle Cells

(see page 39)


(see page 157)



26/212


Cells


Human Tracheal Epithelial Cells (HTEpC) 500,000 cryopreserved cells500,000 proliferating cells C-12644C-12645

HTEpC Pellet 1x106cells in RNAlater C-14064

Human Bronchial Epithelial Cells

(HBEpC)

HBEpC are isolated from the surface

epithelium of human bronchi and stain

positive for cytokeratin.The respiratory epithelia are respon-

sible for the lubrication of the lungs,

the maintenance of humidity, and the

cleaning of the respiratory tract. They

are an important target for drugs, tox-

ins, and carcinogens. In addition, they

are involved in many diseases such as

cystic fibrosis. Consequently, HBEpC are

useful for investigating the function and

pathology of the respiratory system.



(see page 65)



Related products

HBEpCPellet (see page 91)

Human Bronchial Smooth MuscleCells (see page 40)


(see page 157)


Human Bronchial Epithelial Cells (HBEpC) 500,000 cryopreserved cells

500,000 proliferating cells

C-12640

C-12641HBEpC Pellet 1x106cells in RNAlater C-14063

Human Small Airway Epithelial

Cells (HSAEpC)

HSAEpC are isolated from the distal por-

tion of the human respiratory tract in the

1 mm bronchiole area. They stain posi-

tive for cytokeratin.The distal respiratory tract mainly con-

sists of pulmonary alveoli, which are

spherical outcroppings of the respiratory

bronchioles and are the primary sites of

gas exchange with the blood.

HSAEpC are qualified for functional


Small Airway Epithelial Cell

Growth Medium (see page 66)



Related products HSAEpCPellet (see page 92)




(see page 28)


(see page 157)

Fig. 3: HSAEpC culture in phase contrast.


Human Small Airway Epithelial Cells(HSAEpC)


C-12642C-12643

HSAEpC Pellet 1x106cells in RNAlater C-14065

studies to investigate disorders such as

asthma or pulmonary inflammation.





27/212


HumanPrimary

Cells

Human Mammary Epithelial Cells

(HMEpC)

HMEpC are isolated from the human

adult mammary gland and stain positivefor cytokeratin.

Hormones extensively control the de-

velopment of the mammary glands.

Throughout puberty, the glands begin to

develop in females in response to ovarian

hormones. Further on during pregnancy,

rising levels of estrogen and progester-

one cause additional growth and differ-

entiation. Finally, due to the presence of

the hormone prolactin in late pregnancy,

milk secretion (lactation) begins.

Since mammary gland cells can easily be

induced to grow and multiply by hor-

mones, HMEpC are very well suited for

developmental investigations. In addi-tion, they are useful for breast cancer in

vitroresearch since breast cancer usually

originates in the lobules or ducts of the

mammary glands.


Mammary Epithelial Cell Growth

Medium (see page 67)


Cryo-SFM (see page 79) Fig. 4: HMEpC culture in phase contrast.

Related products

HMEpC Pellet (see page 91)


(see page 157)


Human Mammary Epithelial Cells(HMEpC)


C-12650C-12651

HMEpC Pellet 1x106cells in RNAlater C-14066

Human Renal Epithelial Cells

(HREpC)

HREpC are isolated from the adult kid-

ney and stain positive for cytokeratin.

Kidneys have a very complex structure

and contain diverse epithelia such as

those in the convoluted tubules, the

cortical collecting ducts, the calyxes,

Fig. 5: Schematic structure of a kidney.

Ureter

Renal pelvis

Renal calices

Renal medulla

Renale capsule

Renal cortex

Fig. 6: HREpC culture in phase contrast.


Renal Epithelial Cell Growth

Medium 2 (see page 68)



Related products

HREpC Pellet (see page 92)

Human Renal Cortical Epithelial Cells

(see page 26)


(see page 157)

and the renal pelvis. The epithelial cells

present in the distinct ducts and tubules

differ in their morphology and physio-

logical function. Consequently, HREpC

are comprised of a heterogeneous popu-

lation of epithelial cells.

Important physiological processes like

osmoregulation and excretion can be

studied with HREpC in vitro.



28/212


29/212


HumanPrimary

Cells

1.6 Fibroblasts

Applications

Wound healing research

Tissue engineering research

Regeneration studies Toxicological research

Oncology

Epithelial-mesenchymal interaction

studies

Features

CD90 positive


Available from different tissues

Fibroblasts are the most common cellsin connective tissue. Their main function

is to maintain the structural integrity of

the connective tissue by continuously

secreting extracellular matrix proteins

Fig. 1: NHDF culture in phase contrast.

Fig. 2: NHDF culture stained for CD90.

Nuclei counterstained with DAPI.

like collagens, glycosaminoglycans,

and glycoproteins. The composition of

the extracellular matrix determines the

physical properties of connective tissues.

Fibroblasts are morphologically hete-

rogeneous with diverse appearances

depending on their location in the body

and their activity. Injury of tissue displays

a proliferative stimulus for fibroblasts and

induces them to produce wound healing

proteins. Therefore, fibroblasts are well

suited for wound healing studies.

Available Fibroblasts:

NHDF (see below)

HPF (see page 28) HAoAF (see page 28)

HCF (see page 28)

HUF (see page 29)

HVMF (see page 29)

Normal Human Dermal Fibroblasts

(NHDF)

NHDF are isolated from the dermis of

juvenile foreskin or adult skin from dif-ferent locations like the face, the breasts,

the abdomen, and the thighs*. They can

be used for wound healing studies and

dermatological research to investigate

diseases like scleroderma, fibrosarcoma,

fibrosis, xeroderma pigmentosum, and

histiocytoma. Moreover, fibroblasts are

important for cancer research, tissue

regeneration, and tissue engineering

studies.

Recommended media & reagents for

NHDF juvenile foreskin

Fibroblast Growth Medium

(see page 70)


Recommended media & reagents for

NHDF adult donor

Fibroblast Growth Medium 2

(see page 70)



Related products

NHDFPellet (see page 92)

NHDFadult Pellet (see page 92)

Normal Human Epidermal

Keratinocytes (see page 32) Normal Human Epidermal

Melanocytes (see page 33)


(see page 157)

FGF-1 and FGF-2 (see page 179)


Normal Human Dermal Fibroblasts (NHDF)juvenile foreskin


C-12300C-12350

NHDF Pellet 1x106cells in RNAlater C-14030

Normal Human Dermal Fibroblasts (NHDF)adult donor


C-12302C-12352

NHDF adult Pellet 1x106cells in RNAlater C-14031

* Normal Human Epidermal Keratinocytes (NHEK) and Normal Human Epidermal Melanocytes (NHEM) cultured in M2 medium from the same donor areavailable on request.



30/212


Cells


(HPF)

HPF are isolated from human lung tis-

sue. They are often used for studying

lung tissue repair, tissue remodeling afterinjury, or the response after pulmonary

inflammation. Fibroblast disorders like

pulmonary fibrosis or hypoxia-induced


Human Pulmonary Fibroblasts (HPF) 500,000 cryopreserved cells500,000 proliferating cells

C-12360C-12361

HPF Pellet 1x106cells in RNAlater C-14035

pulmonary hypertension can also be in-

vestigated with cultured HPF.





Related products

HPFPellet (see page 92)



Human Small Airway Epithelial Cells

(see page 24) PromoFectin Transfection Reagent

(see page 157)


Human Aortic Adventitial

Fibroblasts (HAoAF)

HAoAF are isolated from human aortic

adventitial tissue. HAoAF can be used

for studies concerning vascular diseases

e.g. neointimal formation, vascular re-

modeling, atherosclerosis, and hyper-

tension. They are also often used for the

examination of fibroblast disorders like


Human Aortic Adventitial Fibroblasts(HAoAF)


C-12380C-12381

HAoAF Pellet 1x106cells in RNAlater C-14037

fibrosis or other diseases linked to either

imperfect or excessive accumulation of

fibroblasts.



(see page 70)



Related products

HAoAF Pellet (see page 91)


(see page 15)

Human Aortic Smooth Muscle Cells

(see page 37)


(see page 157)


Human Cardiac Fibroblasts (HCF)

HCF are isolated from the ventricles of

an adult heart. They play a central role in

the maintenance of the extracellular ma-

trix in the normal heart and the synthesis

of growth factors and cytokines. Under

pathophysiological conditions, cardiac

fibroblasts are involved in scar formation


Human Cardiac Fibroblasts (HCF) 500,000 cryopreserved cells500,000 proliferating cells

C-12375C-12377

HCF Pellet 1x106cells in RNAlater C-14036

after myocardial infarction, cardiac fibro-sis, and cardiac hypertrophy. HCF can be

used to study such processes in vitro.



(see page 71)



Related products HCFPellet (see page 91)

Human Cardiac Myocytes (see page 11)




(see page 157)






31/212


HumanPrimary

Cells

Human Uterine Fibroblasts (HUF)

HUF are isolated from the uterine wall.

During pregnancy, the uterine wall un-

dergoes deep but reversible structural

and biochemical changes. The uterinewall is the target of hormones and

growth factors that act simultaneously

and lead to extensive modifications of

the extracellular matrix. This and other

morphological and biochemical effects

can be studied in vitrousing HUF.


Human Uterine Fibroblasts (HUF) 500,000 cryopreserved cells500,000 proliferating cells

C-12385C-12386

HUF Pellet 1x106cells in RNAlater C-14038



(see page 70)



Related products

HUF Pellet (see page 92)

Human Uterine Microvascular

Endothelial Cell (see page 21)

Human Uterine Smooth Muscle Cells

(see page 41) PromoFectin Transfection Reagent

(see page 157)


Human Villous Mesenchymal

Fibroblasts (HVMF)

HVMF are isolated from human villous

placental tissue. They can be used asmodel systems in organ development

research. In addition, they are useful for

investigating the mechanism regulating

human placental growth and function.


Human Villous Mesenchymal Fibroblasts

(HVMF)

500,000 cryopreserved cells


C-12390

C-12391HVMF Pellet 1x106cells in RNAlater C-14034



(see page 70)



Related products

HVMF Pellet (see page 92)

Human Placental Epithelial Cells

(see page 26)

PromoFectin Transfection Reagent(see page 157)





32/212


Cells

1.7 Follicle Dermal Papilla Cells

Applications

Hair growth research

Drug testing

Toxicological testing

Features

Alkaline phosphatase positive


Available from adult lateral scalp

Available from donors with different

hair colors

Human Follicle Dermal Papilla Cells

(HFDPC) are isolated from human der-

mis originating from the lateral scalp.Information on donor hair and skin color

is available for each lot. HFDPC stain

positive for alkaline phosphatase.

Dermal papillae are embedded in a

laminin and collagen IV rich extracellular

matrix at the base of the hair follicles.

They are essential for the induction and

maintenance of hair growth. Since they

have androgen receptors, they can be

used for in vitroscreening of androgen

blocking reagents.


Human Follicle Dermal Papilla Cells(HFDPC)


C-12071C-12072

HFDPC Pellet 1x106

cells in RNAlater

C-14005


Follicle Dermal Papilla Cell Growth

Medium (see page 72)



Related products

HFDPC Pellet (see page 91)


(see page 157)

Fig. 2: Diagram showing the location of the dermal papilla in the hair follicle.

Sebaceous gland

BulgeHair shaft

Matrix cells

Dermal papilla

Fig. 1: HFDPC culture in phase contrast.



33/212


HumanPrimary

Cells

1.8 Hepatocytes

Applications

Drug testing

Toxicological testing

Metabolism studies Liver physiology research

Liver pathology research

Oncology

Features

Cryopreserved immediately after

isolation

3 - 6 x 106viable cells per cryovial

Adherence > 50%

Phase I reaction positive

Phase II reaction positive Albumin positive

Human Hepatocytes (HHpC) are iso-

lated from the normal livers of single

donors. After isolation, the cells are

immediately cryopreserved and then

intensively quality tested. Test cri-

teria are cell viability, cell adherence

efficiency, phase I metabolism, phase II

metabolism, and albumin synthesis. The

cell adherence efficiency is at least 50%.

The liver is the main metabolic organ

in the body performing glycolysis,

glycogenesis, amino acid metabolism,

lipid metabolism, and ureagenesis. Inaddition, it also regulates the blood

sugar level. Furthermore, hepatocytes

eliminate toxic substances soon after

absorption since the nutrition rich blood

from the gastro-intestinal system first

has to pass the liver. This detoxifica-

tion consists of phase I and phase II

reactions. The most important phase I

enzyme system is the cytochrome P450

system. Consequently, cultured human

hepatocytes are perfectly suited for in

vitro metabolism and toxicity/detoxi-fication studies prior to preclinical or

clinical tests.

Note: For culturing Hepatocytes,

Collagen type I coated culture vessels

are necessary.

Under standard in vitro cell culture

conditions, mature human hepatocytes

usually do not survive for longer than

10-14 days and do not proliferate.


Human Hepatocytes (HHpC) 3 - 6 x 106cryopreserved cells C-12850

HHpC Pellet 1x106cells in RNAlater C-14085

Fig 1. HHpC culture in phase contrast.

Fig. 2: Flow cytometric analysis of PromoCell HHpC

using 7-AAD staining. 77 % viable hepatocytes after

thawing of the cryopreserved cells. CellLabQuanta

SCTM,

BeckmanCoulter,Inc.

0

7-AAD

10 102 103 104

Count

Living cells Dead cells

25

0

12,5

37,5


Hepatocyte Growth Medium

(see page 73)

Hepatocyte Maintenance Medium



Related products

HHpC Pellet (see page 91)

PromoFectin-Hepatocyte Transfection




34/212


Cells

body and minimizes moisture loss.

Keratinocytes are also able to produce

a variety of cytokines, growth factors,

interleukins, and complement factors.

Therefore keratinocytes are importantfor wound healing, inflammation, and

immune response.


Keratinocyte Growth Medium 2

(see page 74)



1.9 Keratinocytes

Applications

Wound healing research

Epidermal development and

differentiation studies Dermatological research

Drug uptake studies

Pharmaceutical testing

Cosmetic and toxicological testing

Oncology

Features

Cytokeratin positive

Available from juvenile foreskin or

adult skin from different locations

Available from single and pooleddonors

Normal Human Epidermal Keratino-

cytes (NHEK) are available from single or

pooled donors isolated from the epidermis

of juvenile foreskin or adult skin from dif-

ferent locations like the face, the breasts,

the abdomen, and the thighs*. They are

the major cell type in the epidermis, mak-

ing up about 90% of the cells.

Epidermal keratinocytes originate in thestratum basale and move up through

the layers of the epidermis. During this

movement, they undergo gradual differ-

entiation and morphology changes until

they reach the stratum corneum, where

they form a layer of nucleus-free, flat,

and highly keratinized squamous cells.

This layer forms an effective barrier to

the entry of infectious agents into the

Fig. 2: NHEK culture in phase contrast.

Note the cobblestone shape typical for

keratinocytes.

Fig. 3: NHEK culture stained for Cyto-

keratin. Nuclei counterstained with

DAPI.

Related products

NHEK.f single donor Pellet (see page 92)

NHEK.f pooled Pellet (see page 92)

NHEK adult, single donor Pellet

(see page 92) NHEK adult, pooled Pellet

(see page 92)


(see page 27)


Melanocytes (see page 33)


(see page 157)

Fig. 1: Structure of the epidermis.

Stratum corneum

Stratum granulosum

Stratum spinosum

Stratum basale


Normal Human Epidermal Keratinocytes(NHEK) juvenile foreskin, single donor


C-12001C-12002

NHEK.f single donor Pellet 1x106cells in RNAlater C-14001

Normal Human Epidermal Keratinocytes(NHEK) juvenile foreskin, pooled


C-12005C-12007

NHEK.f pooled Pellet 1x106cells in RNAlater C-14003

Normal Human Epidermal Keratinocytes

(NHEK) adult, single donor

500,000 cryopreserved cells


C-12003

C-12004NHEK adult, single donor Pellet 1x106cells in RNAlater C-14002

Normal Human Epidermal Keratinocytes(NHEK) adult, pooled


C-12006C-12008

NHEK adult, pooled Pellet 1x106cells in RNAlater C-14004

* Normal Human Dermal Fibroblasts (NHDF) and Normal Human Epidermal Melanocytes (NHEM) cultured in M2 medium from the same donor are availableon request.



35/212


HumanPrimary

Cells

1.10 Melanocytes

Applications

Dermatological research

Melanoma research

Melanogenesis studies Cosmetic and toxicological testing

Skin compound screening

Features

Available from juvenile foreskin or

adult skin from different locations

Available from light, moderate, and

dark skin types

Donor hair and eye color available on

request

Mel-5 positive Cultured in PMA-containing or

PMA-free Medium

Normal Human Epidermal Melanocytes

(NHEM) are isolated from the epidermis

of juvenile foreskin or adult skin from

different locations including the face, the

breasts, the abdomen, and the thighs*.

They are located in the stratum basale,

but branch out between the keratino-

cytes in suprabasal layers (see Fig. 1).

About 5-10 % of the cells in the epider-mis are melanocytes.

The main purpose of melanocytes is

the production of melanin, the protein

responsible for the pigmentation of the

skin, eyes, and hair. Melanin protects the

cells in the skin and in deeper layers from

the hazardous effects of UV radiation. It

is produced and stored in melanosomes,

which are located close to the melano-

cyte membrane, but it can also be trans-

ferred to neighboring keratinocytes.

PromoCell melanocytes are isolated

using either the serum-free, PMA (Phor-

bol Myristate Acetate)-containing Mela-

nocyte Growth Medium or the serum-

free and PMA-free Melanocyte Growth

Medium M2.Since PMA is a tumor promoting mito-

gen, it can interfere with experimental

approaches. Therefore, we recom-

mend using cells isolated in Melanocyte

Growth Medium M2 and also using this

medium for cultivation.


Melanocyte Growth Medium

(see page 75)

Melanocyte Growth Medium M2

(see page 75)



Related products

NHEM.f Pellet (see page 92)

NHEM.f M2 Pellet (see page 92)

NHEM M2 adult Pellet (see page 92)


(see page 27)


Keratinocytes (see page 32)


(see page 157)


Normal Human Epidermal Melanocytes(NHEM) juvenile foreskin


C-12400C-12450

NHEM.f Pellet 1x106cells in RNAlater C-14040

Normal Human Epidermal Melanocytes(NHEM) juvenile foreskin, cultured in M2

Medium


C-12402C-12452

NHEM.f M2 Pellet 1x106cells in RNAlater C-14042

Normal Human Epidermal Melanocytes(NHEM) adult donor, cultured in M2Medium


C-12403C-12453

NHEM M2 adult Pellet 1x106cells in RNAlater C-14043

Fig. 2: NHEM cultured in Melanocyte

Growth Medium in phase contrast.

Fig. 3: NHEM cultured in Melanocyte

Growth Medium M2 in phase contrast.

Fig. 1: Structure of the epidermis with

the location of a melanocyte.

Stratum corneum

Stratum granulosum

Stratum spinosum

Stratum basale

MelanosomaMelanocyteKeratinocytes

* Normal Human Epidermal Keratinocytes (NHEK) and Normal Human Dermal Fibroblasts (NHDF) from the same donor are available on request.



36/212


Cells

1.11 Osteoblasts

Applications

Bone differentiation

Bone remodeling

Bone physiology research Oncology

Inflammatory studies

Osteoporosis research

Features

From knee and hip femoral tissue


Alkaline phosphatase positive

Tested for mineralization

Growth and Mineralization Media

available


Human Osteoblasts (HOB) 500,000 cryopreserved cells


C-12720

C-12760HOB Pellet 1x106cells in RNAlater C-14071


Osteoblast Growth Medium

(see page 76)

Osteoblast Mineralization Medium



Related products

HOB Pellet (see page 92)


(see page 157)

Fig. 1: HOB culture in phase contrast. Fig. 2: Alkaline phosphatase activity in

HOB stained with BCIP/NBT.

Human Osteoblasts (HOB) are isolated

from femoral trabecular bone tissue from

the knee or hip joint region (lot specific

source information is available on re-

quest). They stain positive for alkalinephosphatase and can be mineralized us-

ing PromoCell Mineralization Medium.

Osteoblasts are of mesenchymal origin

and are mainly responsible for bone for-

mation and remodeling. In vivo, they

produce a collagen type I rich extracellular

matrix called the osteoid.

During their development, osteoblasts

undergo a maturation process called

osteogenesis. When they become en-

cased in the bone matrix, they stop prolif-

erating and become osteocytes.

Fig. 3: Mineralized HOB. Calcium de-

posits stained with Alizarin Red S.

For more information please seewww.promocell.com/knowledge-base



37/212


HumanPrimary

Cells

Applications

Proliferation/differentiation studies

Physiological and pathological

research Fat metabolism research

Obesity/diabetes studies

Oncology (e.g. breast cancer)

Features

From subcutaneous and visceral

adipose tissue

Available from different locations


Differentiation capacity to adipocytes

Growth and Differentiation Mediaavailable

Human White Preadipocytes (HWP) are

isolated from adult subcutaneous or vis-

ceral adipose tissue from different loca-

tions (lot specific source information is

available on request).

1.12 Preadipocytes

Adipose tissue is crucial in energy storage

and metabolic homeostasis. An increase

in adipose tissue results either from an

enlargement of mature adipocytes or

from the differentiation of adipocyteprecursor cells (preadipocytes) into new

mature adipocytes. These preadipocytes

are already present in adipose tissues.

They can proliferate throughout adult

life and replace the cells that have dif-

ferentiated into mature adipocytes.

Since this differentiation process is con-

trolled by a variety of growth factors and

hormones, preadipocytes are suitable

for the investigation of the physiologi-

cal mechanisms controlling proliferation,

differentiation, and function of adiposetissue.

The PromoCell adipocyte cell culture

system provides preadipocytes with op-

timized growth media and serum-free

differentiation media.

Fig. 1: Left: Undifferentiated HWP (phase contrast). Middle: HWP after in vitro differentiation into adipocytes using the PromoCell

Preadipocyte Differentiation Medium (phase contrast). Right: HWP after in vitro differentiation into adipocytes (Lipid droplets

stained with Oil Red O).


Human White Preadipocytes (HWP)subcutaneous


C-12730C-12731

HWP subcutaneous Pellet 1x106cells in RNAlater C-14072

Human White Preadipocytes (HWP)visceral


C-12732C-12733

HWP visceral Pellet 1x106cells in RNAlater C-14073


Preadipocyte Growth Medium

(see page 77)

Preadipocyte Differentiation Medium

(see page 77) Adipocyte Nutrition Medium

(see page 77)



Related products

HWP subcutaneous Pellet

(see page 92)

HWP visceral Pellet (see page 92)


(see page 157)



38/212


39/212


HumanPrimary

Cells

1.14 Smooth Muscle Cells

Applications

Organ specific drug testing

Cell-extracellular matrix interaction

studies Physiological and biochemical studies

Oncology

Pathological studies

(e.g. hypertension)

Atherosclerosis research

Features

Smooth muscle -actin positive

CD90 negative

Routinely morphology tested


Smooth muscle cells, which form non-

striated muscles, are structurally and

functionally different from skeletal and

cardiac muscle cells. They are located in

Fig. 1: HTSMC culture in phase contrast.

the walls of blood vessels and hollow or-

gans like the bladder, the uterus, and the

gastrointestinal tract and are responsible

for involuntary movements like peristal-

tic contractions, which propel food alongthe gastrointestinal tract.

PromoCell Smooth Muscle Cells are

available from the aorta, the coronary

artery, the pulmonary artery, the umbili-

cal artery, the trachea, the bronchi, the

bladder, the prostate, and the uterus.

Available Smooth Muscle Cells:

HAoSMC (see below)

HCASMC (see page 38)

HPASMC (see page 38)

HUASMC (see page 39) HTSMC (see page 39)

HBSMC (see page 40)

HBdSMC (see page 40)

HPrSMC (see page 41)

HUtSMC (see page 41)

Fig. 2: HTSMC culture stained for smooth

muscle -actin. Nuclei counterstained

with DAPI.


Human Aortic Smooth Muscle Cells(HAoSMC)


C-12533C-12532

HAoSMC Pellet 1x106cells in RNAlater C-14053

Human Aortic Smooth Muscle

Cells (HAoSMC)

HAoSMC are isolated from plaque-freeregions of the human aorta (see Fig. 3)

and stain positive for smooth muscle

-actin. The pulsatile pressure produced

by the heart causes a cyclic distention

of the aorta. The smooth muscle cells in

the aortic wall subsequently contract it

again. Changes in the arterial wall, asso-

ciated with vascular diseases such as ath-

erosclerosis and hypertension, strongly

influence this process.

HAoSMC are suitable for studying therole of smooth muscle cells under normal

or disease conditions in vitro.


Smooth Muscle Cell Growth




Fig. 3: Diagram of a blood vessel.

Related products

HAoSMC Pellet (see page 91)

Human Aortic Adventitial Fibroblasts

(see page 28)


(see page 15)


(see page 157)

Tunica intima(Endothelial cells)

Tunica externa(Connective tissue)

Tunica media(Smooth muscle cells)



40/212


41/212


HumanPrimary

Cells

Human Umbilical Artery Smooth

Muscle Cells (HUASMC)

HUASMC are isolated from the arteries

of the umbilical cord and stain positivefor smooth muscle -actin. Physiological

and biochemical processes of vascular

diseases can be analyzed with HUASMC

in culture.


Human Umbilical Artery Smooth MuscleCells (HUASMC)


C-12500C-12550

HUASMC Pellet 1x106cells in RNAlater C-14050

Related products

HUASMC Pellet (see page 92)

Human Umbilical Artery Endothelial

Cells (see page 14)







Human Tracheal Smooth Muscle

Cells (HTSMC)

HTSMC are isolated from the trachea

(see Fig. 6) from single donors and stain

positive for smooth muscle -actin. Theyconnect the endings of the tracheal

cartilage by a contractile bridge called

the paries membranaceus and conse-

quently regulate the airway lumen. Un-

der pathological conditions like asthma,

the amount of smooth muscle cells in

the trachea walls is increased leading to

smooth muscle hypertrophy or hyper-

plasia. Hence, HTSMC are suitable for in

vitrostudies of asthma and other pulmo-

nary diseases.






Related products

HTSMC Pellet (see page 92)

Human Tracheal Epithelial Cells

(see page 23)


(see page 157)

Human Tracheal Smooth Muscle Cells(HTSMC)


C-12565C-12566

HTSMC Pellet 1x106cells in RNAlater C-14056

Fig. 6: Cross section through the trachea with c-shaped cartilage connected by the

tracheal muscles.

Pariesmembranaceus

Hyaline cartilage

Mucosa





42/212


Cells

Human Bronchial Smooth Muscle

Cells (HBSMC)

HBSMC are isolated from the bronchi

from single donors and stain positive forsmooth muscle -actin. They connect

the endings of the bronchial cartilage

and consequently regulate the airway lu-

men. Under pathological conditions like

asthma, the amount of smooth muscle

cells in the bronchi walls is increased

Related products

HBSMC Pellet (see page 91)

Human Bronchial Epithelial Cells

(see page 24)


leading to smooth muscle hypertrophy

or hyperplasia. Hence, HBSMC are suit-

able for in vitro studies of asthma and

other pulmonary diseases.






Fig. 7: Diagram of the bladder.


Human Bronchial Smooth Muscle Cells(HBSMC) 500,000 cryopreserved cells500,000 proliferating cells C-12561C-12562

HBSMC Pellet 1x106cells in RNAlater C-14055

Human Bladder Smooth Muscle

Cells (HBdSMC)

HBdSMC are isolated from the detrusor

muscle from the urinary bladder (see

Fig. 7) of single donors and stain posi-tive for smooth muscle -actin. The thick

bladder wall contains smooth muscle cells

arranged in muscle bundles, which empty

the bladder by coordinated contraction.

HBdSMC can be used for in vitrostudies

of physiolo

Documents

PromoCellKatalog2013_lowres