Upload
molly-fox
View
247
Download
2
Tags:
Embed Size (px)
Citation preview
Prokaryote Taxonomy & DiversityClassification, Nomenclature & Identification
Phenetic Classification
Molecular Phylogeny Approach
• Classification (hierarchical grouping of taxa, based on characteristics)
• Nomenclature (formal naming of taxa)
• Identification (define characteristics that match a particular taxa).
• Phylogeny (study of evolutionary relationships)
• Bergey’s Manual of Systematic Bacteriology1st ed. (1984); mostly phenetic classification; 4 volumes 2nd ed. (in prep); mostly phylogenetic classification; 5 volumes
Species Concept for Prokaryotes
Defined differently than Eukarya (no sexual reproduction) = genetically unique population with many stable characters distinctly different from other groups.
Strains are variations within a species; characterized by minor differences in biochemical/ physiological properties (biovars), antigenic distinctions (serovars), shape (morphovars), or viral susceptibility (phage-typing).
Phenetic Characters:
3) Morphological Characters
4) Physiological and Metabolic Characters
Very useful in identification!
1) Ecological Characters
2) Genetic Characters
Phenetic IdentificationUse of multi-test kits and their databases.The combination of positive results from an unknown is
entered into a database of results form known bacteria. A computer model predicts the most probable match and level of certainty. Intended use is for clinical isolates.
Molecular Characters
• Fatty acid profiles (FAME analysis)
• Proteins – Electrophoretic Mobility– Immuno-Reactivity– A.A. Sequence Data
• Nucleic Acids– Nucleotide composition (G+C content ≈ Tm)– Degree of Hybridization (>70% ≈ species)– Nucleotide Sequence Data
Phylogenetic Classification Molecular Chronometers
• Phylogeny refers to grouping based on evolutionary relatedness; regardless of phenetic characters.
• Phylogeny is inferred from changes in protein or rRNA sequence over time.
• Attributes of an Ideal “Molecular Chronometer”:– Universally distributed.– Functionally homologous.– Ease of analysis.– Rate of sequence change commensurate with
evolutionary distance measured.