Project Objectives:

Technical Approach: Accomplishments/Impact/Transitions:

Integrated Enzyme-Logic systems for Monitoring and Treating Autonomously

Injured SoldiersJoseph Wang (UCSD) and Evgeny Katz (Clarkson University)

400 μm

400 μm

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To develop next-generation ‘sense and treat’ autonomous devices for enhancing the survival rate among injured soldiers in the battlefield.

Our approach involves the design, optimization, and evaluation of novel biochemical cascades composed of concatenated enzyme-based logic gates capable of performing Boolean logic operations on biomarker inputs. This system will be applied to the autonomous processing of physiological information and interfaced with electronic transducers & signal-responsive drug-delivery materials / actuators. By using multiple key injury biomarkers as inputs for the enzyme gates, the biochemical logic system provides high-fidelity diagnostics when compared with single biomarker sensors.

Extend enzyme logic gate functionality towards relevant injuries in undiluted human serum samples with high fidelity Developed enzymatic filters to enhance separation in readouts of different input combinations.First demonstration of the applicability of enzyme gates in a porcine model of blunt liver injury First demonstration of minimally-invasive microneedle sensor arrays for monitoring fluctuations in key metabolites within the transdermal fluid Developed wearable textile-based electronic transducers, μ-electronic backbone to control logic gatesDeveloped signal-responsive materials and interfaces actuated by enzyme-based injury related gates for targeted drug delivery and sustained drug release formulation

Impact – Development of autonomous body-worn devices /systems for minimally-invasive biomarker sampling Transitioning towards ‘Pharmacy-on-Demand’ paradigm for the controlled release of a therapeutic intervention in response to signals generated by enzyme logic sensor