Progress in PhotosynthesisResearch

Volume 4

Proceedings of the Vllth International Congress on Photosynthesis

Providence, Rhode Island, USA, August 10-15, 1986

edited by

J. BIGGINS

Division of Biology and Medicine, Brown University

Providence, RI 02912, USA

UNIVERSITATSBIBUOTHEKHANNOVER

TECHNISCHE

INFORMATIONSBIBLIOTHEKJ

1987 MARTINUS NIJHOFF PUBLISHERS kJ

a member of the KLUWER ACADEMIC PUBLISHERS GROUP HHjDORDRECHT / BOSTON / LANCASTER

P

Vil

CONTENTS TO VOLUME IV

1. Photoinhibition

Environmental Stress and Photosynthesis 1Gunnar Oquist

High-irradiance Stress in Higher Plants and Interaction withOther Stress Factors 11

Olle Bjorkman

Photoinhibition of PhotosynthesisStudies on Mechanisms of Damage and Protection in Chloroplasts 19G. Heinrich Krause, Henri k Laasch

Alteration of Electron Flow around Pf)S(l: The Effect onPhotoinhibition 27

Robyn E. Cleland, Christa Critchley, Anastasios Melis

Weak Light Photoinhibition of PSII and its Light DependentRecovery 31David W. Becker, Franklin E. Callahan, George M. Cheniae

Chlorophyll Photobleaching is Dependent on Photosystem IIInhibition 35

Peter J. Dominy, W. Patrick Williams

Photoinhibition at Chilling Temperatures in Intact Leaves andIsolated Chloroplasts of Barley 39G.E. Richards, D.O. Hall

Effects of Bicarbonate on Photoinhibition in Isolated Pea

Chloroplasts 43Jack J.S. van Rensen, Cor J. Klaver

Modification of the Photochemical Apparatus in Maize by

Photoinhibitory Stress at Low Temperature 47M. Percival, M. Bradbury, D.B. Hayden, N.R. Baker

Photoinhibitory Stress Causes Accumulation of a 31 KilodaltonProtein in the Chloroplast Light-harvesting Apparatus 51

Donald B. Hayden, Neil R. Baker, Michael P. Percival

The Kinetics of Photosystem 2 Photoinactivation in Whole Cells,

Thylakoids and PS2 Particles of a Thermophilic Blue Green AlgaSynechococcus Elongatus 55

Ivan Setlik, Ladislav Nedbal, Eva Seth'kova, Danuse Sofrova,Jiff Masojidek

VIII

Photoinhibition of Isolated Chloroplasts and Protoplasts 59Peter Horton, Pam Lee, Alan Hague

EPR Analysis of Photosystem I Degradation during Photoinhibition

of Isolated Chloroplast Membranes 63C.P. Santos, R. Cammack, D.O. Hall

A High C02-rcquiring Mutant of Arabidopsis Thaliana that

Displays Photooxidation in Air 67

Nancy N. Artus, Chris Somerville

Inhibition of Photosystem 2 by Thylakoid Membrane Phosphorylation 71Dawn Ross, Nigel K. Packham

Can Protein Phosphorylation Alleviate Photoinhibition of ThylakoidPhotochemical Activities? 75

Dimah Z. Habash, Neil R. Baker

Heat Shock Proteins Partially Protect against Photoinhibition of

Chlamydomonas Reinhardtii during Heat Shock 79

Gadi Schuster, Dena Even, Klaus Kloppstech, Itzhak Ohad

Light Regulation of Recovery from Photoinhibition 87

K. Gjertsen, S. Nilsen

Photoinhibition and Recovery in Intact Leaves of Pisum SativumGrown in High and Low Light Intensity 91Mohinder Bhogal, James Barber

Involvement of the QB Binding Protein (Mr 32000) in the Adaptationof the Photosynthetic Apparatus to Light Intensity 95F. Koenig

2. Temperature

Comparative Studies on Effects of Low Temperature on ChlorophyllFluorescence Induction Kinetics and Photochemical Activities of

Cucumber and Winter Wheat 99

Dai Yun-ling, Xu Chun-hui, Zhao Fu-hong

Effect of Chilling on the Activity of Enzymes of the PhotosyntheticCarbon Reduction Cycle 103

G.F. Sassenrath, D.R. Ort, A.R. Portis, Jr.

Stress Resistance of Thylakoids: Mechanical Frccze-thaw Damageand Effects of Frost Hardening 107Dirk K. Hincha, Ulrich Heber, Jiirgen M. Schmitt

Acclimation of Light-harvesting and Light-utilization Capacities in

Response to Growth Temperature 111

Kenneth L. Steffen, Jiwan P. Palta

IX

Thylakoid Membrane Composition and Photoinactivation of CO,Fixation in Moss Protonemata as Influenced by the GrowthTemperature 115E.-M. Aro, S. Somersalo, P. Karunen

Phosphatidylglycerol Content and Composition Influence In Vitro

Oligomerization of Purified LHCII from Winter Rye 119N.P.A. Huner, Z. Krupa, J.P. Williams, E, Maissan

3-transhexadecenoic Acid Content and LHCII Organization duringChloroplasl Biogenesis at Low Temperature 123M. Krol, N.P.A. Huner, LP. Williams, E. Maissan

In Vivo Low Temperature-induced Decrease in 3-transhexadecenoicAcid Influences Oligomerization of LHCII 127J.P. Williams, N.P.A. Huner, M. Krol, E. Maissan, P.S. Low,D. Roberts, J.E. Thompson

Chilling Dependent Photoinhibition of Photosynthetic CO-, Uptake 131S.P. Long, A. Nugawela, G. Bongi, P.K. Farage

Damage to Maize Photosynthesis in the Field during Periods when

Chilling is Combined with High Photon Fluxes 139P.K. Farage, S.P. Long

A Chloroplast Heat Shock Protein has Homology to CytoplasmicHeat Shock Proteins 143

Elizabeth Vierling, James K. Roberts, Ronald T. Nagao, Joe L. Key

3. Water Potentials

Magnesium and the Acclimation of Photosynthesis toLow Leaf Water Potentials 147

J.S. Boyer, I.M. Rao

In situ Measurements of the Inhibitory Effects of Low Leaf WaterPotentials on Photophosphorylation 153Adriana Ortiz-Lopez, Donald R. Ort, John S. Boyer

Effects of Water Stress on In Vivo Photosynthetic Biochemistry(Maximum Photosynthesis, Quantum Yield and 77K Fluorescence) 157

Ben Gui-ying, C. Barry Osmond, Thomas D. Sharkey

Contribution of Osmotic Adjustment to the Maintenance of

Photosynthesis during Water Stress 161

Mervyn M. Ludlow

Photosynthesis under Osmotic Stress

A Possible Cause of the Osmotic Stress Induced Susceptibility toPhotoinhibition 169

B. Genty, J.B. Vieira Da Silva

X

Effect of High Leaf K+ on Gas Exchange of Water StressedWheat Leaves 173

Paul A. Pier, Gerald Berkowitz

Solute Accumulation in Chloroplasts during in situ Water Stress 177Ashima Sen-gupta, Gerald Berkowitz

Water-stress Effects on CO, Exchange in Two Grapevine Cultivars 181

M.M. Chaves, M.L. Rodrigues

4. Salinity and Nutrient Deficiency

Phosphoenolpyruvate (PEP) as a Possible Endogenic ProtectingAgent of PEP Using Enzymes against Inactivation by Salt 185Adiva Shomer-Ilan, Delila Moualem-Beno

Targets of Salinity Stress in Spinach 189

Jeanne E. Guenther, Anastasios Melis

The Response of Dunaliella to Salt Stress: A Comparison of Effectson Photosynthesis, and on the Intracellular Levels of the

Osmoregulatory Solute Glycerol, the Adenine Nucleotides and the

Pyridine Nucleotides 193

R. McC. Lilley, A. Goyal, T. Marengo, A.D. Brown

Effects of Long-term and Transient Changes in Salinity on

Photosynthesis, Leaf Demography and Growth in

Plantago Maritima L. 197

Lawrence B. Flanagan

Photosynthetic Pigment Composition of Higher Plants Grown underIron Stress 201

Emilio Monge, Jesus Val, Luis Heras, Javier Abadia

Responses of Photosynthesis and Leaf Angle to Water and Nutrient

Stress in Soybean 205

Irwin N. Forseth

5. Regulation of Gas Exchange

Genetic Variation in the Relationship between Photosynthetic CO,Assimilation Rate and Stomatal Conductance to Water Loss 209

G.D. Farquhar, K.T. Hubick, I. Terashima, A.G. Condon,R.A. Richards

The Measurement and Significance of CO,-gas Exchange Transientsin Leaves 213

Richard B. Peterson

XI

A Model Predicting Stomatal Conductance and its Contribution to theControl of Photosynthesis under Different Environmental Conditions 221J. Timothy Ball, Ian E. Woodrow, Joseph A. Berry

A General Expression for the Control of the Rate of PhotosyntheticCO, Fixation by Stomata, the Boundary Layer andRadiation Exchange 225Ian E. Woodrow, J. Timothy Ball, Joseph A. Berry

Gas Exchange of Flag Leaves and Ears of Wheat. Interpretation on

the Basis of a Photosynthesis Model 229

H. Selinger, D. Knoppik, A. Ziegler-Jons

Characteristics of the CO, Exchange of Wheat Ears 233

A. Ziegler-Jons, D. Knoppik, H. Selinger

Transients of the O, Gas-exchange of Cryptocoryne Ciliata:

The Response to Different pH during Cultivation 237

H.R. Bolhar-Nordenkampf, U. Jager

An Artificial Leaf for Checking Accuracy in the Estimation of

Internal Partial Pressure of CO, in Gas-exchange Measurement 241

G. Bongi, S.C. Wong

Gas Exchange Studies of Carboxylation Kinetics in Intact Leaves 245

A. Laisk, O. Kiirats, H. Eichelmann, V. Oja

Effects of Short-term and Long-term Exposures to Varying CO,

Concentrations on Soybean Photosynthesis 253

William J. Campbell, L.H. Allen, Jr., George Bowes

Photosynthetic Utilization of Lightflecks by Tropical Forest Plants 257

Robert W. Pearcy, Robin L. Chazdon, Miko U.F. Kirschbaum

A Model of Photosynthesis for Isobilateral Leaves IncorporatingBiochemical Constraints and the Light Gradient within the Leaf 261

Miko U.F. Kirschbaum

A Minireview: Comparative Biochemistry of Photosynthesis in

Palisade Cells, Spongy Cells, and Guard Cells of C, Leaves 265

William H. Outlaw Jr.

The Guard Cell Chloroplast: Properties and Function 273

E. Zeiger, K. Gotow, B. Mawson, S. Taylor

Carboxylation of Ribulose 1,5-bisphosphate Inhibited after

Application of the Phytohormone, Abscisic Acid, to Whole Leaves of

Xanthium Strumarium (C3) and Zea Mays (C4) 281

Klaus Raschkc, Elke Fischer

The Ratio of RuBP Carboxylase to Oxygenase Activity and

Photosynthetic Gas Exchange 283

F. Maechler, B. Lehnherr, J. Mueller, J. Noesberger

XII

6. Mechanisms of CO, Concentration

Inorganic Carbon Fluxes and Photosynthesis in Cyanobacteria -

a Quantitative Model 289

Leonora Reinhold, Menekhem Zviman, Aaron Kaplan

A Model for Inorganic Carbon Accumulation in Cyanobacteria 297

Teruo Ogawa, Aaron Kaplan

The Mechanism of Inorganic Carbon Uptake by Cyanobacteria:

Energization and Activation by Light 301

A. Kaplan, Y. Marcus, D. Zenvirth, T. Omata, L. Reinhold,

T. Ogawa

Immunochemical Studies on the Major Proteins in CytoplasmicMembranes of Cyanobacteria 309

Tatsuo Omata, Tcruo Ogawa

Na+-independent HCO,~ Uptake: An Additional Mode of InorganicCarbon Acquisition in Cyanobacteria 313

George S. Espie, David T. Canvin

The Role of Carbonic Anhydrasc in the Inorganic Carbon

Concentrating System of Chiamydomonas Reinhardtii 317

FI. David Husic, James V. Moroney, N.E. Tolbert

Regulation of Carbonic Anhydrasc Expression in Chiamydomonas 325

Jane Bailly, John R. Coleman

Photosynthetic Characteristics of Several High-CO,-requiring Mutants

of Chiamydomonas 329

Kensaku Suzuki, Martin H. Spalding

Diffusion and Active Transport of Inorganic Carbon Species in

Freshwater and Marine Macroalgae 333

John A. Raven, Andrew M. Johnston, Jeffrey J. MacFarlane,

Misni Bin Surif, Shona Mclnory

Photosynthetic Responses to Oxygen and Inorganic Carbon of Low-

and High-CO,-grown Cells of Chara Corallina 341

William J. Lucas and Franqois Brechignac

Inorganic Carbon Concentrating Systems from anEnvironmental Perspective 345

George Bowes, Julia B. Reiskind

Evidence for Bicarbonate Active Transport in Elodea NuUallii 353

T. Taylor Eighmy, Leland S. Jahnke, Wayne R. Fagerberg

Evidence of a CO,-concentrating Mechanism in C4 and Some G,-C4Intermediate Flaveria Species 357Brandon D. Moore, Maurice S.B. Ku, Gerald E. Edwards

XIII

7. Crop Productivity

Variation in Photosynthetic Characteristics among Triticum Speciesand Attempts to Exploit it in Breeding 361R.B. Austin, M.A. Ford, T.E. Miller, C.L. Morgan, M.A.J. Parry

Photosynthesis, Photosynthate Partitioning, and Productivity of

Temperate Forage Legumes 369G.H. Heichel, M.H. Hall, C.C. Sheaffer

Barriers to Increasing Crop Productivity by Genetic Improvement in

Photosynthesis 377

Roger M. Gifford

Genetic Control of Photosynthesis in Relation to Growth of Pea

(Pisum Sativum L.) Plants 385John D. Mahon, Shaun L.A. Hobbs

Effect of Potassium on Photosynthetic Apparatus andYield Components of Wheat Plant 393

N.I. Ashour, A.O.M. Saad, A.T. Thalooth, T.A. Nour

Relationship between Certain Photosynthetic Parameters and Yield inDifferent Wheat Cultivars 395

M.R. Saric, Z.S. Stankovic, B. Krstic

Growth Kinetics and Photosynthetic 0,-evolution by Chlorella

Immobilized in Ca-alginate 399

LP. Callegari, L. van den Broeck, E. Simkcns, B. de Wannemaeker,

J.P. Simon

Effect of Growth Regulators on the Growth, Chlorophyll

Development and Productivity of Euphorbia Lathyris L:

A Hydrocarbon Yielding Plant 403

J. Garg, A. Kumar

Sugar Beet Factory Wastewater Treatment in a High Rate

Oxidation Pond 407

J. Taha, B. El Hamouri

Photosynthetic Capacity, Leaf Development, Growth and

Productivity of Sugar Beet under Optimal Nitrogen Supply 411

R. Ceulemans, H. Vandendriessche, J. Feyen, I. Impens

Equations to Define Canopy Photosynthesis from Quantum

Efficiency, Maximum Leaf Rate, Light Extinction, Leaf Area Index,

and Photon Flux Density 415

K.J. Boote, J.W. Jones

Effects of CO, Enrichment Preconditioning on Chlorophylls Contents

and Photosynthetic CO, Exchange in Tomato Leaves 419

R.A. Khavari-Nejad

XIV

8. Biosynthesis of Photosynthetic Pigments

Regulation of Chlorophyll Biosynthesis. Genetics and Biochemistry ofDelta-aminolevulinate Synthesis 423

Wei-yeh Wang, Dinq-Ding Huang, Tien-En Chang,Deborah Stachon, Becky Wegmann

Biosynthetic Precursors of 6-aminolevulinic Acid in

Plants and Algae 431

Jon D. Weinstein, Mark A. Schneegurt, Samuel I. Beale

Formation of 6-aminolcvulinic Acid from Glutamic Acid in AlgalExtracts: Fractionation of Activities and Biological Constraints on theRNA Requirement 435Jon D. Weinstein, Sandra M. Mayer, Samuel I. Beale

Non Equivalence of Glutamic and 6-aminolevulinic Acids asSubstrates for Protochlorophyllidc and Chlorophyll Biosynthesisin Darkness 439

Baishnab C. Tripathy, Constantin A. Rcbeiz

Two pathways of 5-aminolcvulinic Acid Biosynthesis Destined forFormation of Bactcriochlorophylls in Photosynthetic Bacteria 445Tamiko Oh-Hama, Haruo Seto, Shigetoh Miyachi

Cloning and Expression of a Gene Complementing 5-aminolevulinateDehydratase Deletion in E. Coli Strain SHSP3. 449A.P. Balange, A.-M. Delaunay, C. Huault

Subcellular Localisation of Porphyrin Synthesis Enzymes inPea and Arum 453

Alison G. Smith

Oxidation of Protoporphyrinogen to Protoporphyrin, a Step in

Chlorophyll and Heme Biosynthesis: Purification and PartialCharacterization of the Enzyme from Barley Mitochondriaand Etioplasts 457Judith M. Jacobs, Nicholas J. Jacobs

Chlorophyll Synthetase and its Implication for Regulation of

Chlorophyll Biosynthesis 461Wolfhart Rikliger

Photoreactions in Chloroplast Development 469W. Trevor Griffiths, Caroline J. Walker

The Purification and Properties of Three Latter-stage Enzymes of

Chlorophyll Synthesis 475William R. Richards, Margaret Fung, Andreas N. Wessler,Shivu B. Hinchigeri

Protochlorophyllidc Reduction in Anabaena 483Heather Adamson, Caroline Walker, Annette Bees, Trevor Griffiths

XV

Comparison of Chlorophyll Accumulation and 14C-ala Incorporationinto Chlorophyll in Dark and Light in Green Barley 487Nicolle Packer, Heather Adamson, Jane Walmsley

Chlorophyll RC I, its Structure, Function and Biosynthesis 491

Horst Senger, Dieter Dornemann, Kiriakos Kotzabasis,Mathias Senge, Werner Schmidt, Margit Wissenbach

Tetrypyrrole Turnover in Plants 499

George Hendry

Alterations in Photosynthetic Pigment Synthesis in Tissue Cultured

Tobacco Callus 503

Elizabeth A. Davis, Kevin B. Clairmont, William G. Hagar

A Method for Determining Phycocyanin and ChlorophyllConcentrations in Whole Cell Suspensions of Cyanidium Caldarium 507

J.D. Houghton, LA. Holroyd, S.B. Brown

9. Photoregulation of Chloroplast Development

Light Regulation of Plastid Gene Expression during Chloroplast

Biogenesis in Barley; Evidence for Regulation of Translation and

Post-transcriptional Determination of mRNA Levels 511

John E. Mullet, Scott K. Boyer, Tineke Berends, Robert R. Klein

Photoregulation of Maize Plastid Genes during Light-induced

Development 519

Steven R. Rodermel, Lyle D. Crossland, Jean H. Lukens,

Karen M. Muskavitch, David R. Russell, J.Y. Shen, Y.-ShengZhu,

Lawrence Bogorad

Light Regulation of Genes for the Large and Small Subunits of

Ribulose-bisphosphate Carboxylase in Tobacco527

Marilyn S. Abbott, Lawrence Bogorad

Light Regulation and Localization of OEC-33 in Maize 531

Ann Sutton, Leslie Sieburth, John Bennett

Protochlorophyllide Reductase and the Faillure of Corn to Acclimate

to Low Irradianccs 535

J. Bennett, J.R. Schwender, E.K. Shaw

Chloroplast Development and Regulation of LHCP-gene Expressionin Greening Cultured Soybean Cells 539

G. Erdos, K. Shinohara, H.-Q. Chen, S. Lee, M. Gillott,

D.E. Buetow

Accumulation and Degradation of P700-chlorophyll a Protein

Complex, Plastocyanin, and Cytochrome bjf Complex543

Teruhiro Tabake, Hajime Kawamura, Hiroshi Ishikawa,

Satsuki Kubo, Tetsuko Takabe

XVI

Biosynthesis of Chloroplast Ribosomal Components 547R. Mache, H. Audren, C. Bisanz-Seyer, A.M. Dome, O. Massenet,C. Rozier, F. Thomas

Acclimation of Rubisco Activity and Quantity to Different Irradianceswithin a Same Tobacco Leaf. Differential Expression in SSU

and LSU mRNA 553

J.L. Prioul, A. Reyss

Pyruvate, Pi Dikinase mRNAs of Maize 557Donald R. Hague, Pamela D. Collins

Changes in the Appearance of Ribulose-1.5-bisphosphate-carboxylase

(RuBPCase) during Senescence of Mustard Cotyledons 561

Helga Kasemir, Detlef Rosemann, Ralf Oelmuller

Translational Regulation of Light-induced Ribulose 1,5-bisphosphateCarboxylase Gene Expression in Amaranth 565James O. Berry, Basil J. Nikolau, John P. Carr, Daniel F. Klessig

Transport and Processing of Ferredoxin and Plastocyanin:A Thylakoid-spccific Processing Enzyme 569Peter Weisbeek, Johan Hageman, Ken Kecgstra, Colin Robinson,

Sjef Smeekcns

Some Requirements for the Insertion of the Precursor of Apoproteinsof Lemna Light-harvesting Complex II into Barley Thylakoids 573

Parag R. Chitnis, Rachel Nechushtai, Eitan Harel, J. Philip Thornber

Synthesis of Chlorophyll-binding Polypeptides during Greening ofEtiolated Barley 577

Michael J. White, Beverley R. Green

Evidence for Translational Regulation of Euglena Protein Synthesis

by Light 581A. Rikin, A. Monroy, S.D. Schwartzbach

Synthesis of Chloroplast Proteins in Chiamydomonas 585A. Boschetti, E. Breidenbach, J. Clemetson-Nussbaum, S. Leu,

H.P. Michel

Synthesis of Polypeptides Associated with PSI by the Isolated

Sorghum Vulgare Chloroplasts 589R. Mannar Mannan, M. Krishnan, A. Gnanam

In vitro Synthesis of Heat Shock Proteins by Chloroplasts 593M. Krishnan, S. Krishnasamy, R. Mannar Mannan, A. Gnanam

Development of the Photosystem I Light-harvesting Apparatusin Wheat 597

Neil R. Baker, Gu J. Bredenkamp

A Chlorophyll B Deficient Mutant of Wheat with an Altered

Photoadaptation Response 601Keith D. Allen, Murray E. Duysen, L. Andrew Staehelin

XVII

Euglena Ferredoxin NADP Reductase, LHCP Apoprotein, and theirPhotocontrol during Chloroplast Development 605Anthony Spano, Harris Ghaus, Jerome A. Schiff

Dynamic Morphological Changes of Proplastids and Mitochondria inDark-organotrophically Grown Cells of Euglena Gracilis Transferredto an Inorganic Medium 609T. Osafune, S. Tukada, S. Sumida, T. Ehara, E. Hase

Photoreduction of NADP+ during Early Greening of EtiolatedBean Leaves 613M. Bertrand, A. Radunz, E. Dujardin

10. Chloroplast Molecular Genetics

Chloroplast Genes for Photosynthetic Membrane Components 617J.C. Gray, E.R. Blyden, CJ. Eccles, P.P.J. Dunn, S.M. Hird,A.-S. Hoglund, T.M. Kaethner, A.G. Smith, D.L. Willey, T.A. DyerCharacterisation of Genes for Components of Photosystem II inWheat Chloroplast DNA 625S.M. Hird, R.F. Barker, G.R.M. Courlice, D.L. Willey, T.A. Dyer,J.C. Gray

Biogenesis of Photosystem II Complex in Spinach Chloroplasts 629Akira Watanabe, Ei-ichi Minami, Makoto Murase, Kenji Shinohara,Tomohiko Kuwabara, Norio Murata

Photosystem II Biogenesis: Roles of Nuclear Gene Products 637G.W. Schmidt, L.E. Sieburth, K.H. Jensen, K.L. Greer,

F.G. Plumley, D.L. Herrin

Chloroplast Gene Expression in Chloroplast Ribosome-deficientMutants of Chiamydomonas Reinhardtii 645David L. Herrin, Gregory W. Schmidt

A Gene Cluster in the Z. Mays Plastid Genome is Homologous toPart of the S10 Opcron of E. Coli 649

Ignacio M. Larrinua, William E. McLaughlin

Evolution of the 5' and 3' Regions Flanking the tRNA1"" GUG Genein the Dicot Chloroplast Genome: the Role of Insertions/Deletions 653J. Aldrich, B. Cherney, E. Merlin, L. Christopherson, C. Williams

Characterization of the B6/F Complex Subunits and Studies on theLHC-kinase in Chiamydomonas Reinhardtii Using Mutant StrainsAltered in the B6/F Complex 655Claire Lemaire, Jacqueline Girard-Bascou, Francis-Andre Wollman

Molecular Cloning and Nucleotide Sequences of the Genes EncodingCytochrome B-559 from the Cyanelle Genome of

Cyanophora Paradoxa 659Amanda Cantrell, Donald A. Bryant

XVIII

Molecular Characterization of Chiamydomonas Reinhardtti, ac-208: A

Plastocyanin-less Mutant 663Sabeeha Merchant, Lawrence Bogorad, Sheila A. Iverson,John H. Richards

The Gene for the P7()()-chlorophyll a Protein in Euglena Gracilis is

Interrupted by Three Introns 667

J.C. Cushman, R.B. Hallick, C.A. Price

Structural and Functional Relatedness of Chromophytic and

Rhodophytic RuBP Carboxylase Enzymes 671Scott N. Newman, Rose Ann Cattolico

Initial Characterization of Mufr/to/--induced Photosynthesis Mutations 675

W.B. Cook, M.D. Hunt, D. Miles

Failure to Process the Dl Protein Inhibits the Oxidizing Side of PSIIbut not the Reaction Center or Reducing Side Reactions: Analysis ofthe LF-1 Mutant of Scenedcsmus 679

James G. Metz, Himadri Pakrasi, Charles J. Arntzen,

Michael Seibert

DNA Biosynthesis in Chloroplasts and its Regulation: Studies onIsolated Chloroplasts and Chloroplast Extracts 683W. Ronald Mills, Brian J. Baumgartner

Chloroplast-based in vitro Translation System 687I. Kozieradzki, L. Malek

II. Photosynthetic Bacteria

Regulation of Gene Expression and Assembly of the PhotosynlhelicPigment-protein Complexes 691G. Drews, G. Klug, R. Liebetanz, R. Dierstein

Analysis of Transcription through the Rhodobacter Capsulatus pufOperon Using a Translational Fusion of pufM to the E. ColiLacz Gene 699

Carl E. Bauer, Marianne Eleuterio, Debra A. Young,Barry L. Marrs

Molecular Genetics of C-type Cytochromes and of Ubiquinol-cytochrome C-, Oxidoreductase of Rhodopseudomonas Capsulata 707F. Daldal

Molecular Genetics of the Ubiquinohcytochrome C2 Oxidoreductaseat Rhodopseudomonas Capsulata 713

Edgar Davidson, Susan Rook, Fevzi Daldal

Oxygen and Light Regulation of Expression of Genes for LightHarvesting (LH-I, LH-II), Reaction Center (RC-L, RC-M, RC-H),Pigment Biosynthesis and a Transcriptional Role in the Protective

XIX

Function of Carotenoids in Rhodobacter Capsulatus 111Yu Sheng Zhu, John E. Hearst

The Role of the Light-harvesting I Antenna Proteins in the CorrectInsertion of the Photochemical Reaction Center of Rhodobacter

Capsulatus and Rhodobacter Sphaeroides 721

Roger C. Prince, W. Jim Jackson

Genetic and DNA Sequence Analysis of a Rhodobacter CapsulatusMutant Unable to Properly Insert Photochemical Reaction Centersinto the Membrane 725

W. Jim Jackson, Roger C. Prince

Assembly of Photosynthetic Units in Membranes of Rhodobacter

Sphaeroides during a Down-shift in Light Intensity 729Dennis Farrelly, Robert A. Niederman

Cloning and Oxygen Regulated Expression of Genes Coding for theReaction Centre and Light Flarvesting Polypeptides of

Rhodopseudomonas Sphaeroides 733Mark K. Ashby, Shirley A. Coomber, C. Neil Hunter

Cloning and Oxygen Regulated Expression of Genes for the Bacterio-

chlorophyll Biosynthetic Pathway in Rhodopseudomonas Sphaeroides 131

Shirley A. Coomber, Mark K. Ashby, C. Neil Hunter

Isolation of Genes Encoding the Photosynthetic Apparatus of

Chloroflexus 741S.J. Robinson, T.E. Redlinger

Oxygen Regulation of Cytochrome C554 Synthesis in Chloroflexus 745

T. Redlinger, J. Foster, M. Wynn, D. Knaff, R. Blankenship,R.C. Fuller

12. Cyanobacteria

The Cyanobacterial Photosynthetic Apparatus: A Structural and

Functional Analysis Employing Molecular Genetics 749

Donald A. Bryant, Robert de Lorimier, G. Guglielmi,

Veronica L. Stirewalt, Amanda Cantrell, S. Edward Stevens, Jr.

Genes with Mutations Causing Herbicide Resistance from the

Cyanobacterium Synechococcus PCC 7002 757

J.S. Buzby, R.O. Mumma, D.A. Bryant, J. Gingrich,

R.H. Hamilton, R.D. Porter, C.A. Mullin, S.E. Stevens, Jr.

The Effects of Light Intensity and Nitrogen Starvation on the

Phycocyanin Promoter in the Cyanobacterium SynechococcusPCC 7002 761

G.E. Gasparich, J. Buzby, D.A. Bryant, R.D. Porter,

S.E. Stevens, Jr.

XX

Organization of the Genes Encoding Phycoerythrin and the Two

Differentially Expressed Phycocyanins in the CyanobacteriumPseudanabaena PCC 7409 765

James M. Dubbs, Donald A. Bryant

Molecular Cloning and Preliminary Characterization of a recA Genefrom the Cyanobacterium Synechococcus PCC 7002 769

Randy C. Murphy, Donald A. Bryant, Ronald D. Porter

Genetic Analysis of a Cyanobacterial Gene Encoding a MembraneProtein which Accumulates under Iron Stress 773

Louis A. Sherman, K.J. Redely, H.C. Riethman,

George S. Bullerjahn

Cloning and Characterization of a Gene Encoding an Iron-regulatedMembrane Protein in Anacystis Nidulaus R2 777K.J. Reddy, Carolyn Vann, Louis Sherman

The Construction of Hybrid Cloning Vectors and the Improvement of

Transformation Efficiency in the Cyanobacteria, Anacystis NidulansR2 and A. Nidulans R2-SPC 785

Carolyn N. Vann, Lauren A, Playl, Louis A. Sherman

Differential Effects of Iron and Chlorophyll in the Control ofMembrane Synthesis in Anacystis Nidulans 789

James A. Guikema

Polypeptide Rapid Turnover and Photosystem II Recovery fromCalcium Stress in Anacystis Nidulans 793

Jerry J. Brand, Jin-song Sheng

Isolation and Sequence of the Gene for Ferredoxin I from the Cyano¬bacterium Anabacna PCC 7120 795

Jawed Alam, Richard A. Whitaker, David W. Krogmann,Stephanie E. Curtis

Isolation and Nucleotide Sequence Analysis of the Ferredoxin Gene

from the Cyanobacterium Anacystis Nidulans R2 797Michael E. Reith, David E, Laudenbach, Neil A. Straus

Cyanobacterial Genes for the Cytochrome B6-F Complex:Sequence Homology with Plastid and Bacterial Genes but Divergenceof Operon Structure 801Toivo Kallas, Susan Spiller, Richard Malkin

Site-directed Mutagenesis in the Photosystem 11 Gene psbD,

Encoding the D2 Protein 805Wim F.J. Vermaas, John G.K. Williams, Dexter A. Chisholm,Charles J. Arntzen

Nucleotide Sequences of Both psbD Genes from the CyanobacteriumSynechocystis 6803 809John G.K. Williams, Dexter A. Chisholm

XXI

Genetically Engineered Cytochrome B559 Mutants of theCyanobacterium, Synechocystis 6803 813Himadri B. Pakrasi, John G.K. Williams, Charles J. Arntzen

Phycocyanin Genes in the Cyanobacterium Synechocystis 6701 and aPotential Gene Rearrangement in a Pigment Variant 817Lamont K. Anderson, Arthur R. Grossman

Diuron Resistance in the PSBA Multigene Family of AnacystisNidulans R2 821

Judy A. Brusslan, Susan S. Golden, Robert Haselkorn

Functioning of Photosystem II in Mutant Strains of the

Cyanobacterium Anacystis Nidulans R2 825Howard Robinson, Susan Golden, Judy Brusslan, Robert Haselkorn

Contiguous m/Gene Organization in a HeterocystousCyanobacterium 829

Barry Saville, Neil Straus, John R. Coleman

Molecular Cloning of a recA-like Gene from the CyanobacteriumAnabaena Variabilis 833

George W. Owttrim, John R. Coleman

Cyanobacterial Transformation: Expression of Col El Plasmids in

Anacystis Nidulans 6301 837H. Daniell, G. Sarojini, B.A. McFadden

Genetic and Biochemical Analysis of Cyanobacteria Defective in

Photosynthetic Oxygen Evolution 841Valdis A. Dzelzkalns, Lawrence Bogorad

Index of names 845