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PRODUCTION OF HIGHLY PURIFIED BOVINE
TRANSFERRIN
Group 5BArjun Goel, Connie Marcelli,
Lianne Kark, Sara Dang, Vivien Tai
Faculty Advisor Prof C.A. Mims
TimetableTask
3 10 17 24 1 8 15 22 29 5 12 19 26Obtain Chemical & Physical Info and Thermodynamic & Kinetic DataAlternatives Available within design briefFirst ReportScalingProject Background
24-Sep
Second Report--Scope of Design; Timetable of Design; Allocation of Jobs within Team
9-OctProcess Flow Diagram NO MASS & ENERGY BALANCES
22-Oct Basis of Design Note
26-Oct
Frozen PFD w/ Mass & Energy Balances; Major Process Streams; Major Process EquipmentSpecification of Process Equipment--Sizing Based on Mass Balance; Auxiliary Equipment Specification; Material Selection; Waste recycle/treatment/disposalKEY UNIT DESIGNP&ID SafetyEnvironmental ImpactControlControl & Operating Philosophy--Normal OperationControl & Operating Philosophy--Startup/Shutdown OperationPlant Layout--General; Safety & Fire; Spacing RecommendationsCost Estimates--Capital Costs; Operating Costs; Selling Price/Unit Price; IRR/NPV analysisEconomic FeasibilityEnergy Efficiency
1-Nov Scheme of Final Report9-Nov Present Preliminary Layout
26-Nov Final Report & DrawingsProcess UtilitiesRecommendationsSocio Economic ImpactEditorExecutive Summary
September October November
Agenda
Mass Balances & Waste Treatment
Key Unit Discussion
Unit Specifications
Current Challenges
Process Overview
Crude Paste
Filtration
Chromatography
Dry Transferrin Powder
Agenda
Mass Balances & Waste Treatment
Key Unit Discussion
Unit Specifications
Current Challenges
Mass Balance — FeedsStarting Paste
18 kg
D.I. Water 571 L
Celite 6 kg
FeCl3, NaHCO3, Na-Citrate for iron Binding
Diafiltration BuffersWash for
Column
EDTA for Iron Chelating
Process
Distilled Water 160 L
Mass Balance — Products
Water 740 L
Celite 6 kg
Inorganics FeCl3, NaHCO3,
Na-Citrate, EDTA, NaOH
Purified Apotransferrin 350g / Batch
Process
Trace Proteins
Ethanol 4.5 kg
Solids Liquids
Immunoglobins for recovery
Waste Treatment--LiquidsOption 1 Option 2
NeutralizeAcid/Base Addition
To Sewer
Waste Water
Solid Waste
Waste Water
Process
Reverse Osmosis
Ion Exchange
Waste Treatment — Solids
1. Reverse Osmosis cartridges
2. Filter press cakes
Disposal via contractor
• Mainly salts and celite trapped in filters
Agenda
Mass Balances & Waste Treatment
Key Unit Discussion
Unit Specifications
Current Challenges
Key Unit: R-101
Wet Paste
2
4
5
R-101
Celite Filter Aid
3
Deionized water
NaOH
P-101
1
Key Unit: R-101
• Stirred Tank• Cylindrical• Conical Bottom • Tank Material: SS316• In Cold Room 0-5 oC
Key Unit: R-101
• Volume of solution in tank: 400 L
• Unit Selected:– Capacity: 757 L ( 200 Gal )– Depth: 1.46 m– Diameter: 0.91m
Key Unit Accessories
• Level Indicator
• pH Monitor
• Temperature Indicator
• SS316 piping
• Valve
• Air Diaphragm Pump
• Mixer
Agenda
Mass Balances &Waste Treatment
Key Unit Discussion
Unit Specifications
Current Challenges
Filter Press: FP-101
• 12” in diameter• Stainless Steel
plates and frames• Cellulose Pads• Four 1” sludge
frames• 40 psi• Cost: $6 703
Filter Press: FP-1013 Applications:
1) 4 Plates: removes 12 000 g paste @ 19 L/min
2) 2 Plates: removes 1 320 g denatured protein @ 9 L/min
3) 2 Plates: collects 3 600 g precipitated protein @ 9 L/min
PrefiltrationSF-101
• Before ultrafiltration
• Millipore low protein binding cartridge filter
• Filtration Area: 0.7m2
• 80 psi
• Entrains particles > 0.5 m
• US$208 for 2
Ultrafiltration
Spiral Wound FilterUF-101
• Concentration: 450 L to 90 L
• Polysulfone
• Flow: 100 L/hr
• Flux: 22 L/m2 hr
• Pressure: 60 PSI
• Cost: $8 212
Spiral Wound FilterUF-102
• Concentrate from 30L to 15L
• Regenerated cellulose Membrane
• Pressure: 60 PSI
• Flow: 10L/hr
• Flux: 11 L/m2hr
• Cost: $2 154
Hollow FiberUF-201
• Concentrate from 12 L to 6 L
• Polysulfone• 25 PSI• 6 L/hr• 30 L/ m2hr• Peristaltic Pump
DEAE Column
1
C-201
To Waste
To Waste5
DEAE Sepharose Fast Flow Column: C-201
• Automated
• Includes: The Column
The Bioprocess Skid
The computer
DEAE Resin
• Total Cost: CND$211, 000
Water Distillation ColumnT-204
• Rate: 190 L/24 hrs• Storage capacity:
280 L• Size:
2m x 1m x 0.6m
• Cost : US$ 3 945
Deionized Water UnitI-101
• Flow range:
15 – 23 L/min• Size:
1.5m x 0.6m x 0.4m• Cost:
US$ 4 142
LyophilizerL-101
• Similar unit used in
plant
• Capacity: 8-12 L• ~6 L of water to
condense per batch
Future Directions
• Key unit development– P&ID– Design equations– Control Philosophy
• Economic Evaluation
• Plant Layout
• Appendices