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5.3 Microbial and Synthetic Approaches to CO2 Utilization,
2017 BIO World Congress
Dr. Hideaki YukawaCEO, Utilization of Carbon Dioxide Institute Co., Ltd.
Production of Biofeeds, Biofuels and Biochemicalsfrom CO2 using Hydrogen Bacteria
July 24, 2017
Copyright© 2017 by UCDI All Rights reserved. 1
UCDI’s Business Plan
Convert CO2 into high-value products
Contribution to food security and global warming problems
Copyright© 2017 by UCDI All Rights reserved. 2
UCDI’s Platform: Hydrogen Bacteria
Hydrogenophilus thermolutelus
Conclusion: 20L-culture could support one Astronaut
Copyright© 2017 by UCDI All Rights reserved. 4
Biotech. and Bioengi. vol-8,217(1966)
NASA: Exploring the possibility of using ”Hydrogen Bacteria” as
a protein production for space flight
Copyright© 2017 by UCDI All Rights reserved. 5
History of UCDI’s Hydrogen Bacteria
Isolation from Nature
1970s: Dr. Kodama’s team, Applied Microbiology Lab., University of Tokyo, had tried to isolate many kinds of hydrogen bacteria from nature.
They set up a landmark of hydrogen bacteria’s feature for future industrialization.
* Rapid growth in a complete inorganic medium Target of doubling time : less than 1 hour
* Thermophilic hydrogen bacteriaTarget of optimum temperature: over 50˚C
Scientific AdvisorDr. Toru Kodama
Copyright© 2017 by UCDI All Rights reserved. 6
History of UCDI’s Hydrogen Bacteria
Screening Result1976: Dr. Kodama’s team got big success
The strain’s feature• Doubling time: 1hour• Optimum temperature: 52˚C• Optimum pH: 7.0•Gram- negative• Strictly aerobic
Taxonomic position: Pseudomonas hydrogenothermophilaAgric. Biol. Chem., 41,685 (1977)
By the detailed taxonomic study: Hydrogenophilus thermolutelusInt. J. Syst Bacteriol.,49,783 (1999)
Tokyo
IzuPeninsula
The targeted strain was isolated from a hot spring in Izu Peninsula
Copyright© 2017 by UCDI All Rights reserved. 7
[archive] History of UCDI’s Hydrogen Bacteria
“Hydrogen Bacteria” is rich nutrition• Protein content: 80%• Continuous production process is
practicable• Feed test using poultry was done
successfully
Social cartoon“Dinner in future”
“Hydrogen Bacteria” on the dinner table
Japanese News Paper “Asahi “ took up the research project in both article and social cartoon(1978)
Copyright© 2017 by UCDI All Rights reserved. 8
World Population Forecast
This sharp increase in global population is the root cause of various issues facing our planet, including environmental pollution and food security.
Now: More than 7 B people inhabit our planet
Future: Over 9B or close to 10 B by the year 2050
Source: Ministry of Agriculture, Forestry and Fisheries
Copyright© 2017 by UCDI All Rights reserved. 9
Biofeeds: Single Cell Protein
UCDI strain Hydrogenophilusthermolutelus
• High growthrate: dubling time 1hour
• High Amino Value• Non-GMO• Natural protein source
High protein-score/ High cell density
Before inoculation 10hrs incubation
Hydrogenophilusthermolutelus
Copyright© 2017 by UCDI All Rights reserved. 11
Biofeeds: Aquaculture Fields
Fishers and aquaculture:
Important sources of food and nutrition
World per capita fish supply
20kg in 2014 Thanks to Aquaculture
Aquaculture provides half of all fish
Source: FAO Fish State
Copyright© 2017 by UCDI All Rights reserved. 12
How to keep sustainable aquaculture?
Fish feed: the biggest cost in the
production of aquaculture
Fishmeal is main ingredient
50~70%W/W in fish feed
Fishmeal Production and Price
Plant engineering : UCDI collaborating with major engineering companies
Copyright© 2017 by UCDI All Rights reserved. 13
UCDI’s Biofeeds : Business Plan
2014 2025 Growth rate
Chicken 88.4 113.0 28%
Pork 111.2 133.0 20%
(Million ton)Supply and Demand
Market : aquaculture, chicken, pork
Source: Policy Research Institute, Ministry of Agriculture, Forestry and Fisheries (PRIMAFF)
Copyright© 2017 by UCDI All Rights reserved. 14
rDNA Research
Established main generic tools
• Genetic manipulation both in autotrophic and heterotrophic culture• Gene transfer
Plasmid vectorsIntroduction into chromosome by homologous recombination
• Transformation by electroporation• Selection
Positive selection by antibiotics resistance kanamycin, hygromycin, etc.
Negative selection with sacB gene• Reporter gene
Beta-galactosidase from Thermus therm ophilus
(e.g. for screening of promoters)
Counter selection
Copyright© 2017 by UCDI All Rights reserved. 15
rDNA Research
Set up ” Industrial host cell ” for cell-factory
Business Plan
Butanol : Material for Jet Fuel
Amino Acids: Feed Additives (Trp, Met. etc.)
Organic Acids/Aromatic compounds : Materials for Polymer
Copyright© 2017 by UCDI All Rights reserved. 16
Biocatalysts: genetically modified strains
We are looking for business partners !!
Amino Acid
Established : August 12, 2015
Paid-in Capital : US$1M
Head Office : Minato-ku, Tokyo
Representative : Dr. Hideaki Yukawa, CEO
Research Facility : Campus of University of Tokyo
Mission : Contribution to Food Security and Global
Warming Problems
Copyright© 2017 by UCDI All Rights reserved. 17
About Us
Copyright© 2017 by UCDI All Rights reserved. 18
Scientific Advisor
Dr. Toru Kodama
Special Advisor
Dr. Kazuo Nagai
Director
Ms. Tomoko YukawaExecutive Officer
Mr. Hiroshi Maeda
Manager
Ms. Setsuko Beppu
Manager
Ms. Sumie HonmaManager
Ms. Hikari Miyazaki
Manager
Ms. Ryoko Senoo
CEO
Dr. Hideaki Yukawa
Head Office
Copyright© 2017 by UCDI All Rights reserved. 19
Research Team
rDNA works
Fermentation works
Senior Researcher
Dr. Naoto OhtaniResearcher
Dr. Kenro ShinagawaResearcher
Dr. Shota Oku
Alignment :Applied Microbiology Lab. University of Tokyo
Professor, PhD
Masaharu Ishii