Prodigiosin Synthesis

UV-induced “Colorless” Mutants of Serratia marcescens

amino acids

a

c

b(g)

d(ws)

Prodigiosinenzyme x

enzyme y

enzyme z

The Normal Pathway to Prodigiosin

O

NH

NH

N

A

C

b(g)

d(ws)

Prodigiosinenzyme x

enzyme y

enzyme zamino acids

Serratia marcescens D1 (wild type)(DNA is not mutated for any of the genes)

a

c

B(g)

D(ws)

Prodigiosinenzyme x

enzyme y

enzyme zamino acids

Serratia marcescens D1 (wild type)(DNA is not mutated for any of the genes)

a

c

b(g)

d(ws)

Prodigiosinenzyme x

enzyme y

enzyme zamino acids

Serratia marcescens D1 (wild type)(DNA is not mutated for any of the genes)

amino acids

a

c

b(g)

d(ws)

Prodigiosinenzyme x

enzyme y

enzyme z

Serratia marcescens ( )(DNA is mutated in enzyme x gene)

A

C

b(g)

d(ws)

Prodigiosinenzyme x

enzyme y

enzyme zamino acids

Serratia marcescens ( )(DNA is mutated in enzyme x gene)

c D(ws)

Prodigiosinenzyme x

enzyme y

enzyme zamino acids

b(g)A

Serratia marcescens ( )(DNA is mutated in enzyme x gene)

c D(ws)

Prodigiosinenzyme x

enzyme y

enzyme zamino acids

b(g)A

Serratia marcescens ( )(DNA is mutated in enzyme x gene)

amino acids

a

c

b(g)

d(ws)

Prodigiosinenzyme x

enzyme y

enzyme z

Serratia marcescens ( )(DNA is mutated in enzyme y gene)

c D(ws)

Prodigiosinenzyme x

enzyme y

enzyme zamino acids

b(g)A

Serratia marcescens ( )(DNA is mutated in enzyme x gene)

amino acids

b(g)

d(ws)

Prodigiosinenzyme x

enzyme y

enzyme z

Serratia marcescens ( )(DNA is mutated in enzyme y gene)

A

C

c D(ws)

Prodigiosinenzyme x

enzyme y

enzyme zamino acids

b(g)A

Serratia marcescens ( )(DNA is mutated in enzyme x gene)

amino acids

d(ws)

Prodigiosinenzyme x

enzyme y

enzyme z

Serratia marcescens ( )(DNA is mutated in enzyme y gene)

C

a B(g)

c D(ws)

Prodigiosinenzyme x

enzyme y

enzyme zamino acids

b(g)A

Serratia marcescens ( )(DNA is mutated in enzyme x gene)

amino acids

d(ws)

Prodigiosinenzyme x

enzyme y

enzyme z

Serratia marcescens ( )(DNA is mutated in enzyme y gene)

C

a B(g)

933 WCF

WCF

933

c D(ws)

Prodigiosinenzyme x

enzyme y

enzyme zamino acids

b(g)A

Serratia marcescens ( )(DNA is mutated in enzyme x gene)

amino acids

d(ws)

Prodigiosinenzyme x

enzyme y

enzyme z

Serratia marcescens ( )(DNA is mutated in enzyme y gene)

C

a B(g)

933 WCF

B(g)

Prodigiosin

B(g)

WCF

933

c D(ws)

Prodigiosinenzyme x

enzyme y

enzyme zamino acids

b(g)A

Serratia marcescens ( )(DNA is mutated in enzyme x gene)

amino acids

d(ws)

Prodigiosinenzyme x

enzyme y

enzyme z

Serratia marcescens ( )(DNA is mutated in enzyme y gene)

C

a B(g)

933 WCF

D(ws)

Prodigiosin

D(ws)

a

c

b(g)

d(ws)

Prodigiosinenzyme x

enzyme y

enzyme zamino acids

Serratia marcescens D1 (wild type)(DNA is not mutated for any of the genes)

D1 WCF

d(ws)

D1 933

Why is D1 (so capable!) such a poor donor to either mutant…

…compared to cross-feeding from either mutant?

933 WCF

933 WCF

D(ws)

B(g)

b(g)