PRES/Pigments/1 DD 14/11/02 NATURAL PIGMENTS PRES/Pigments/1 DD 14/11/02 PIGMENTATION ORIGINATED HISTORICALLY The visual appearance is an important characteristic

PRES/Pigments/1 DD 14/11/02

NATURAL PIGMENTS


PIGMENTATION ORIGINATED HISTORICALLY

The visual appearance is an important characteristic for the consumer.

Pigmentation is associated with healthy animals and quality products.


Layers and broilers are unable to synthesise their own pigments.

Pigments added to the diet to obtain the desired colour

Country preference concerning pigmentation

corn producing countries corn contains natural pigments, xanthophylls free range layers grass contains natural pigments,

xanthophylls


PIGMENTATION

1. Analyse the market needs in terms of colours an economic factor

2. The colour of egg yolks from light-yellow to golden-yellow expressed in the Roche Colour Fan score number

3. The colour of the skin and shank of broilers.


CONTROL OF PIGMENTATION

•Mainly visual observations•They are subjective:

• individual observations

• source of light

• background colour

Colour meter

Chromatometer

3 Dimensional determination


PIGMENTS IN INGREDIENTS

Average xanthophylls (mg/kg) in raw materials

MAIZESORGHUMWHEATSOYBEAN MEALGRASS MEALALFALFA MEALMAIZE GLUTEN FEEDMAIZE GLUTEN 60%

18222

150160

25200


•Xanthophyll level varies in raw materials genetic, field and climate effects harvest and storage conditions

temperature

light

time

•The results on the pigmentation depend on :

•the xanthophyll content and the deposition rate by the

animal.

•The adaptation period for layers is about 5 days.

Pigmentation variables


Oxidation Vitamin E support the absorption (biological antioxidants) Antioxidants in feed to protect the fat and the xanthophylls

from oxidation.

The feed intake

Stress conditions (temperature, etc.)

Diseases

Salmonellosis and coccidiosis damage the mucosa of the

digestive tract and reduce the absorption of carotenoids.

Processing techniques

High temperatures may degrade xanthophylls.

FACTORS AFFECTING PIGMENTATION


WHAT ARE NATURAL PIGMENTS

Natural Pigments are based on natural Carotenoids mainly used in poultry feed. (fish feed is a new opportinuty).

They are obtained from dehydrated, stabilized, homogenized and soaponified Marigold extract (yellow pigments) and Paprica extract (red pigments).


PRODUCTION

1. Flower Meal production

2. Pelletting : To have a better action of the solvent

3. Oleoresin production and extraction of the solvent (ether)

4. Oleoresin saponification (with Potassium)

5. Adsorption on a dry carrier (for powder products)


Saponification:

Hydroxycarotenoids have to be saponified before being absorbed. Saponification improves the digestibility of the original esterified Tagete and Capsicum xantophylls.

Isomerisation:

The most bioavailable pigments are on trans form. To reach a higher percent of trans form, flowers are selected genetically. A chemical processing technique called isomerisation (cis to trans) can also be used


NATURAL and SYNTHETIC

Natural for:

Yellow skin broilers: natural is preferred because gives a uniform gold pigmentation

Vegetal eggs: synthetic pigments are not allowed

Pasta eggs: better colour, more uniform and resistant to heat

General trend:consumers are moving to natural. Researches show doubts about healthiness of synthetic pigments.


NATURAL PIGMENTS MARKET

Quality:

Trans-isomers level

Level of saponification (highest as possible)

Stability against oxidations (vacuum sealed packaging and antioxidants)


STABILITY OF NUTRIGOLD YELLOW DRY

0

20

40

60

80

100

% remaining activity

42 90 155 181

Time (days)

REMAINING ACTIVITY IN ORIGINAL VACUUM PACKAGING STORED AT 23 °C


STABILITY OF NUTRIGOLD YELLOW DRY

0

20

40

60

80

100


1 58 125

Time (days)

REMAINING ACTIVITY IN OPENED BAG STORED AT 23 °C


STABILITY OF NUTRIGOLD RED DRY

0

20

40

60

80

100


1 58 125

Time (days)

REMAINING ACTIVITY IN OPENED BAG STORED AT 23 °C


SYNTHETIC PRODUCTS FOR PIGMENTATION

ROCHE Carophyll Yellow (10%) apo-ester Carophyll Red (10%) canthaxanthin

BASF Lucantin Yellow (10%) apo-ester Lucantin Red (10%) canthaxanthin

Comparison of product efficiency : synthetic : natural = 6 : 11 kg synthetic Yellow = 6 kg NUTRI-GOLD YELLOWIn broiler diets 8 kg NUTRI-GOLD YELLOW can replace 1 kg synthetic product.

In some countries synthetic products are prohibited by law because of safety reasons


NUTRI-GOLD REDDOSE LEVELS FOR EGG YOLK

PIGMENTATION

NUTRI-GOLD RED

ROCHE COLOUR FAN wheat-based * maize-based 30% maize-based 50%

8 - 9

9 - 10

10 - 11

11 - 12

12 - 13

500

800

1400

1800

2200

400

700

1200

1600

2000

200

600

1000

1400

2000

* NUTRI-GOLD YELLOW at 150 g/tonne added for yellow xantophyll level


Pigment dosage in active component considering the requested yolk color

Roche colour scaleDietXantophylls

AddedXant

10 to 11 11 to 12 12 to 13 >13

mg/kg mg/kg

0 red 3.75 5 7.5 10yellow 5 5-10 5-10 10

3 Red 3.75 5 7.5 10yellow 2 2-6 2-6 7

6 Red 3.75 3.75 5.75 10yellow 0 2.5 2.5 4

9 Red 2.5 3.75 5.5 10Yellow 0 0 1 2

12 Red 2.0 3.5 5.5 7.5yellow 0 0 0 0.5


Pigment dosage considering the requested yolk color

Roche colour scaleDietXantophylls

AddedNutrigold

10 to 11 11 to 12 12 to 13 >13

mg/kg (g/Tm)

0 red 750 1000 1500 2000yellow 250 250-500* 250-500* 500

3 Red 750 1000 1500 2000yellow 100 100-300 100-300 350

6 Red 750 750 1150 2000yellow 0 125 125 200

9 Red 500 750 1100 2000Yellow 0 0 50 100

12 Red 400 700 1100 1500yellow 0 0 0 25

*Lower value for lower end of the color range


Several diets are normally used; A starter, grower and finisher diet. It is efficient to have lower xanthophyll levels in the starter - and higher levels in the finisher diet

BROILER DIETS WITH XANTHOPHYLLS FOR A GOLDEN YELLOW SKIN COLOUR

Example of dosage recommendation :

Starter diet - No NUTR-GOLD YELLOW dryGrower diet - 0.5-1.0 kg/t NUTR-GOLD YELLOW dryFinisher diet - 1.0-1.5 kg/t NUTR-GOLD YELLOW dry

The dosage depends on the natural (ca. 15 ppm) xanthophyll level, body weight, feed conversion.For more intensive pigmentation small amounts of red pigments can be added. ( 0.5-1.0 kg/tonne NUTRI-GOLD RED)


EGG YOLK COLOURS

ROCHE COLOUR FAN LUCANTIN CX FORTEg/tonne

10.5 2011.5 2512.5 35

Trial in UKWheat based layer diet (3mg/kg Xantophylls)

WHEAT BASED DIET + 175 g/ton NUTRI-GOLD YELLOW DRY


NUTRI-GOLD YELLOW

DRYTM

INGREDIENTS Extract of Marigold flower (Tagetes erecta), ethoxiquin, mineral carrier

DESCRIPTION Xantophyls of natural origin (Lutein and Zeaxabthin) highly bioavailable (maximum intestinal absorption and tissue deposition)

TECHNICAL SPECIFICATIONS

Xantophylls Trans-lutein Trans-zeaxanthin Criptoxanthin Total carotenoids Epoxide Saponification Color Scent Moisture Physical state Granulometry

20 g/kg Min 80% Max 6% 0.4% min 87% max 11% min 95% Yellow Characteristic Max. 12 % Free flowing powder 90% per mesh 100

DOSAGE From 150 to 2000 g/ton of complete feed, depending on the contents of carotenoids of the feeds and the required color

PACKING 25 kg aluminum bags, vacuum-sealed with nitrogen application

SHELF LIFE 6 months in a dry, cool place in the original packaging

The information in this data sheet is to the best of our knowledge true and accurate but all instructions, recommendations or suggestions are made without guarantee. Since the conditions of use are beyond our control, the manufacturer disclaims all liability for loss or damage suffered from the use of these data or suggestions.


NUTRI-GOLD YELLOW

LIQUIDTM

INGREDIENTS Extract of Marigold flower (Tagetes erecta), ethoxiquin, soft water

DESCRIPTION Xantophyls of natural origin (Lutein and Zeaxabthin) highly bioavailable (maximum intestinal absorption and tissue deposition)


Xantophylls Trans-lutein Trans-zeaxanthin Criptoxanthin Total carotenoids Epoxide Saponification Color Scent Physical state Density Viscosity

15 g/kg Min 80% Max 6% 0.4% min 87% max 11% min 98% Dark Yellow Characteristic Liquid easy to spray 1 g/kg max 25 csk


PACKING 1000 kg plastic containers with nitrogen application

SHELF LIFE 8 months in a cool place in the unopened original packaging. Avoid direct sunlight



NUTRI-GOLD REDTM

INGREDIENTS Extract of paprika (Capsicum annum), ethoxiquin, mineral carrier

DESCRIPTION Compound of red xantophylls, like Capsanthin, highly bioavailable, with maximum intestinal absorption and biological efficiency


Xantophylls Trans-capsanthin Trans-zeaxanthin Capsorubin Criptoxanthine Trans-lutein Color Saponification Moisture Granulometry Granulometry

5 g/kg Min 54% Max 6% 5% 0.06 % max 3% Dark red Min 95% Max 12% 90% per mesh 100


PACKING 25 kg aluminum bags, vacuum-sealed with nitrogen application

SHELF LIFE 6 months in a dry, cool place in the original packaging



Documents

PRES/Pigments/1 DD 14/11/02 NATURAL PIGMENTS PRES/Pigments/1 DD 14/11/02 PIGMENTATION ORIGINATED HISTORICALLY The visual appearance is an important characteristic