METHIONINE SUPPLEMENTATION OPTIONS IN POULTRY

Presented by:Dr. Shivi Maini

Clinical Research- R&D,Ayurvet Limited

Introduction

Scientific Validations and Recommendations

Overview of Synthetic DL Methionine Supplementation in Poultry

Methionine Supplementation Options

Key Features, Benefits and Advantages of Product

Methionine sulphoxide

Methionine sulphoxide

Methionine in natural feedstuff (present in L form) is limited

Low Biological Availability

DL Methionine in basal ration is added to meet

the requirement

Replaceable

Non-Replacea

ble

Storming issues with synthetic amino acid: SCIENTIFIC REPORTS

• Possible synthetic Hazards• Residues in Synthetic Feed Supplements• Unjustified in organic productions• Global Food Safety & Security Concerns

Methionine Toxicity

Sulphate Release

Poor Bone Mineralization

Oxidation of Excess

Methionine

Immunosuppression

Thinning of Egg Shells

Metabolic Acidosis

Low Body Wt Gain

Poor egg and Shell Quality

EXCESS OF SYNTHETIC METHIONINE IN POULTRY FEED MAY HAMPER GROWTH & PERFORMANCE LEADING TO REVERSE EFFECTS.. Diseases of Poultry page No. 1028, Iowa state press

Excess Methionine

FLUCTUATIONS IN DL Methionine PRICE

Sept, 14 Oct,14 Nov,14 Dec,14 Jan, 15 Feb, 15 March,15 April, 15 May,15 June,150

100

200

300

400

500

600

310

535

508

500

495495

480

500

520

535

Price (Rs.) of DLM (99%)

HERBAL FEED ADDITIVES: A SAFE, SCIENTIFIC & SUSTAINABLE

APPROACH

PHYTOADDITIVE REPLACEMENT OPTIONS

• Herbs are known and documented for having highly precursors of vital AA

• Mimics the activity of Methionine etc

Sweeter

Safer

Same taste

Cicer arientinum

Phaseolus mungo

Triticum sativum Mucuna pruriens

Allium cepa

MAJOR CONSTITUENT HERBS WITH BIOMIMICKING ACTION

NATURAL ALTERNATIVE

Contains Standardized Herbal Extract – Mimics the activity of Methionine

Rich in precursors of Methionine/SAMe

Fits strategically in Methionine Cycle in body to provide optimum Function

Non- toxic No residual effects Environmental Friendly Safe to Livestock Safe to user

Novel feed additive

For partial replacement of synthetic methionine

BIOMIMICKING ACITIVITY OF HERBS

DL Methionine

L Dopa Mucuna pruriens

Better MAT Activity

Increases SAMe Formation

.Zhao WQ, Latinwo L, Liu XX, Lee ES, Lamango N, Charlton CG. L-dopa upregulates the expression and activities of methionine adenosyl transferase and catechol-O-methyltransferase. Exp Neurol. 2001 Sep;171(1):127-38.

Methionine ATP

S-Adenosyl Methionine (SAMe)

Transmethylation reaction

Methyl Group

Homocysteine

SAH (S-Adenosyl Homocysteine)

(Allicin) Allium sativa

mimics the activities of cysteine prodrugs such as N-acetyl cysteine (NAC) and S-adenosyl methionine (SAM)

Ref: Bernas GC, Gonzales RE, Solevilla RC, Ysrael MC. Pharmacology-Toxicology. In: Guevara BQ, editor. A guide book to plant screening: Phytochemical and biological. Santo Tomas Philippines: Research Centre for Natural Sciences, university of Santo Tomas; 2004. P. 103-132.

Corcoran GB, Wong BK. Role of glutathione in prevention of acetaminophen-induced hepatotoxicity by N-acetyl-L-cysteine in vivo: studies with N-acetyl-D-cysteine in mice. J Pharmacol Exp Ther 1986;238(1):54-61.

Cicer arientinum

Cicer arientinum enhances the bioavailability of partial or limiting levels of DL Methionine

Reference: Arch Latinoam Nutr. 1975 Dec;25(4):363-75. [Limiting amino acids of chickpeas (Cicer arietinum) for chickens and pigs]. [Article in Spanish] Shimada A, González EA.

Scientific Validation Studies on METHIOREP

16 National Research Trials

4 International Research Trials

4 Thesis (2 MVSc+ 2 PhD)

12 Scientific Publications

SCIENTIFIC VALIDATIONS ON METHIOREP :

BROILER, OUAT Bhubaneswar, Orissa

LAYER, IPPM, TANUVAS, Chennai

PIG GROWERS, PGRIAS, Kattupakam, TN

BROILER, Field trial in 10 farms, Bidar , Karnataka

Layer, 0-40 wk study, PGIVAS, Akola, MS

16

MQA Broiler Study, Udgir, MS

BROILER, Field trial in 10 farms, Bidar , Karnataka

BROILER, Project Directorate of Poultry

BROILER, College of Vet. Sci. Hyderabad: (3 trials)

broiler, Durg Vety College, ChattisgarhLayer Durg Vety College, Chattisgarh

broiler, College of Vet. Sci. , Uttar Pradesh

Cattle, Nagpur vety college , MS

Cattle, Nagpur vety college , MS

Trial conducted by:Dr Borja Vila

RECERCA I TECHNOLOGIA AGROALIMENTARIESMonogastric Nutrition

Department of Animal Nutrition, Centre de Mas BoveI,

Reus, Barcelona, SPAIN.

SCIENTIFIC VALIDATION WITH INTERNATIONAL COLLABORATORS

STUDY INVESTIGATOR: Woo Kyun Kim, Ph.D.Assistant Professor

303 Poultry Science BuildingDepartment of Poultry Science

University of GeorgiaAthens, Georgia 30602

STUDY INVESTIGATOR: Dr. José A. Garcia Regueiro (Ph. D.)

IRTA MonellsFunctionality and Nutritionjoseantonio.garcia@irta.cat

www.irta.cat

Trial conducted by:Dr Borja Vila

RECERCA I TECHNOLOGIA AGROALIMENTARIES

Monogastric NutritionDepartment of Animal

Nutrition, Centre de Mas BoveI,

Reus, Barcelona, SPAIN.

Study 1: Comparative efficacy evaluation of Methiorep & synthetic Methionine in improving

growth at partial replacement

EXPERIEMENTAL DESIGN

660 day old male Ross 308 broiler chicken were procured from commercial hatchery. (5 replicates of 33 chick each/group)

Experiemental duration: 5 weeks

GROUP I - No added synthetic/herbal methionine GROUP II - Synthetic methionine @0.2% (2Kg/tonne

of feed)GROUP III - 1/3 replacement of synthetic

methionine by MethiorepGROUP IV - 1/2 replacement of synthetic

methionine by Methiorep

MEAN BODY WEIGHT (Kg) AT 5th WEEK

Group I Group II Group III Group IV2.2

2.252.3

2.352.4

2.452.5

2.552.6

2.652.7

2.36

2.642.66 2.65

Average live weight at the end of the experiment

Significantly higher body weight was recorded in combination groups III & IV & DLM supplemented group II as compared to

untreated control group I

Average Daily Feed Intake Per Bird

Group I Group II Group III Group IV92949698

100102104106108

97.6

106.5 106103.3

Average daily feed intake (g)

Average daily feed intake non significantly different in treatment groups

FEED EFFICIENCY

Group I Group II Group III

Group IV1.441.461.48

1.51.521.541.561.58

1.61.62

1.607

1.508

1.5089999999999

9

1.5169999999999

9

Non-significa

ntly different among treatme

nts

Dressing Percentage (%)

Group I Group II Group III

Group IV

8181.5

8282.5

83

81.6

82.882.4 82.4

Series1

A non-significant difference in Dressing % was recorded among treated groups II, III & IV, however it was

significantly better than untreated group I

Organoleptic and Sensory Parameters

Sensory Evaluation of Microwave cooked meatIt was done using eight point Scale

Appearance

6.3 6.66

Control

DLM + Methiorep (1:1)

Economics of ProductionPARTICULARS GROUPS

IIIII (DLM + Methiorep)1/3 replacement

IV (DLM + Methiorep)1/2 replacement

Cost of ChickRs. 19/-

Rs. 19/- Rs. 19/-

Avg. Feed consumed/chick (0-42days) 3.73 kg

3.71 kg 3.62 kg

Cost of feed@Rs.25/kgRs. 93.25/-

Rs.92.75/- Rs.90.50/-

Cost of additives DL Methionine@ Rs525/-Methiorep@Rs.135

Rs.3.91 /-R s. 0.324/- + Rs. 2.57

= Rs. 2.89R s 0.48/- + Rs 1.90

= Rs. 2.38

Misc. costRs. 15/-

Rs. 15/- Rs. 15/-

Wt of broiler2.64kg

2.66kg 2.65 kg

Cost of broiler@Rs. 75/kgRs.198/- Rs.199.50 Rs.198.75/-

Profit : Cost of Broiler- (cost of chick+misc. + feed + additive) [198 - (19+93.25+3.91+15)=

Rs. 66.84/- chick

[199.50 - (19+92.75+2.89+15) =

Rs. 69.86/bird

[198.75 - (19+90.50+2.38+15)=

Rs.71.87

Loss due to mortality (per bird)1.5/-

0.74/- 0.72/-

Net profitRs. 65.34/bird

Rs. 69.12/- Rs. 71.15/-

NET PROFIT OVER CONTROL GROUP --- Rs. 3.78/- Rs.5.81/-

CONCLUSION

Partially replacing DL –methionine by Methiorep by 1/3 or ½ was well comparable to DLM supplementation alone in terms of growth performance & carcass yield.

Improving the overall economics of production as Methiorep costs less than DL-Methionine.

Recommended use of Methiorep for multiplying your Profits.

INTERNATIONAL POULTRY SCIENTIFIC FORUMATLANTA, USA, JANUARY 27–28, 2014

Publication

TRIAL STATUS

TITLE OF PROJECT:

Comparative efficacy evaluation of Methiorep and DLM supplementation in broilers

INVESTIGATOR

Dr. Park W. Waldroup, Poultry Science Department, University of Arkansas, Fayetteville AR 72701 PWW- 2010-08

TRIAL RESULT IN BRIEF:

Study 2. International trial on Methiorep : USA

Results of these studies shows that herbal methionine product (Methiorep) can be used for partial replacement of Synthetic methionine in broiler diet. It appears to work in combination with dl methionine (67/33) at 0.3% level of inclusion in supporting body weight

gain of the broiler chick; which is cost effective on basis CB Analysis..

Study3: International trial Methiorep

STUDY INVESTIGATOR:

Woo Kyun Kim, Ph.D.Assistant Professor

303 Poultry Science BuildingDepartment of Poultry Science

University of GeorgiaAthens, Georgia 30602

INTESTINAL AMINO ACID TRANSPORTERS:

°AT-1 (SLC family 6 member 19) and LAT-1 (SLC family 7 member 7)

LIVER NUTRIENT METABOLISMS USING REAL-TIME PCR:

IFN-gamma Glucose metabolism (PEPCK) Pyruvate Kinase (PK)Glucose Transporter 2 (GLUT2) Pyruvate Dehyrogenase (PDH)) Lipid metabolism (Acetyl-CoA acetyltransferase 2 (ACAT2) Peroxisome Proliferator Receptor-gamma (PPAR-gamma)Lipoprotein Lipase (LPL), Fatty acid synthase (FAS)

OBJECTIVE : To evaluate the effects of herbal Methiorep on pullet growth, feed efficiency, immunity, amino acid transporters, and liver nutrient metabolisms in pullets.

To evaluate the effects of herbal Methiorep on feed efficiency, egg production and egg quality in laying hens

PARAMETERS STUDIED: Growth, Performance & Production Feed consumption Body weight Feed efficiency Mortality uptil 18 weeks Egg production Egg quality parameters

GENE EXPRESSION STUDIES RELATED TO IMMUNE SYSTEMS: IL-10 TLR-4 IL-1 IL-6

2: International trial Methiorep

Experiemental Design

Study I (0-40 wk): This study will be conducted to evaluate the effects of herbal

Methiorep on pullet growth, feed efficiency, immunity, amino acid transporters

and liver nutrient metabolisms in pullets.

A total of 208 1-day old Leghorn pullets (4 trt. x 6 rep x 13 birds per cage) will be

randomly allocated to 4 treatment groups:

1) Negative control (No synthetic DL-Met supplemented)

2) Positive control ( 0.2% DL-Meth supplemented)

3) DL-Met (50%) + herbal Methiorep (50%)

4) DL-Met (67%) + herbal Methiorep (33%)

Summary of Results: S.NO.

PARAMETERS (AVG.) CONTROL GROUP

(WITHOUT DLM)

DLM (0.2%) METHIOREP (0.1%)

+ DLM (0.1%)

METHIOREP (0.133%)+ DLM

(0.067%)

1 FEED EFFICIENCY (19-40 WK)

2.26 1.92 1.94 1.95

2 AVG. EGG PRODUCTION % 91.08 96.24 97.42 96.67

3 MEAN EGG WEIGHT (G) 52.75 56.40 56.06 56.07

4 MEAN SPECIFIC GRAVITY 1.085 1.087 1.088 1.088

5 Mean Haugh Unit (22-40 weeks)

98.62 97.98 98.40 99.85

6 YOLK COLOUR BRIGHTNESS 58.36 58.41 58.36 58.01

7 YOLK COLOUR REDNESS 3.84 3.94 3.91 3.93

8 SHELL WEIGHT (G) 5.27 5.54 5.42 5.49

Feedback on Product usage experience by Investigator

Study 4: International trial Methiorep

STUDY INVESTIGATOR:

Dr. José A. Garcia Regueiro (Ph. D.)IRTA Monells

Functionality and Nutritionjoseantonio.garcia@irta.cat

www.irta.cat

OXIDATIVE STATUS

TBARS Antioxidant enzymatic profile

OTHER METABOLITES

Carnitine Taurine 5-methyl- THF Vitamin B12 VITAMIN B6 Adrenaline Total fatty acids Glucose Total Protein

OBJECTIVE : The effect of two products on specific biomarkers and related metabolites in blood from a poultry animal model.

PARAMETERS TESTED:

Metabolite Assay HomocysteineCysteineGlu-CysCys-Gly GSHGSSG.

Amino acid profile in plasma: SAMe Using HPLC/ DAD & MS/MS

Identification of chemical markers of herbal substances

International Trial: Methiorep

VALIDATIONS SUMMARY

• The most important result was a positive correlation between Methionine/ Methiorep

Cysteine and Homo-Cysteine with B12, this is an expected result but could be useful as a way

to validate the effect of the treatments.

• Rutin & L DOPA was isolated from Methiorep supplemented group.

• Ratios Cys/Met and Ser/Met:

Ratio Cys/Met :was lower for DL-Methionine (2.03 at 360 h) and that for of DL-

Methionine+Methiorep (2.26 at 360 h).

Ser/Met ratio showed a similar pattern with the lowest value for DL-Methionine (3.32 at 360

h) and that for DL-Methionine+Methiorep showed a value of 3.74 at 360 h.

• These results evidenced a strong effect of DL-Methionine in the concentration of Methionine

that requires a lowest quantity of Ser to produce Methionine.

STUDY 5: STUDIES ON COMPARATIVE EFFICACY OF HERBAL AND

SYNTHETIC METHIONINE ON PERFORMANCE OF VEN COB BROILER

PhD THESIS (2011) Submitted at COLLEGE OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY, ANJORA, DURG (C.G.) 491001, INDIRA GANDHI KRISHI VISHWAVIDYALAYA, RAIPUR (C.G.)

Albumin mg/dl Serum Globulin g/dl Serum total protein g/dl

SGPT U/L0123456789

Basal Diet

Herbal methionine

DL Methionine

Effect of herbal & synthetic methionine on Blood biochemical constituents

Hb g/dl PCV % TLC Heterophil Lymphocytes0

10

20

30

40

50

60

9

29.5

19.56

38.25

50.75

9.6528.75

18.27

37

50.75

9.81

28.33

18.85

37

52.75

Basal Diet

Herbal methionine

DL Methionine

DLM 2

DLM + HM

DLM 1

Effect of Methionine supplementation on Haematological parameters

CONCLUSION

The growth performance, feed efficiency &

biochemical parameters in DLM & Methiorep

supplemented group II are well comparable to DLM supplementation alone

(group I)

STUDY 6: COMPARATIVE EFFICACY OF SUPPLEMENTATION OF SYNTHETIC AMINO ACIDS

WITH HERBAL ALTERNATIVES IN LAYERS

PhD Thesis :

submitted to Deptt. of Animal Nutrition,

College of Veterinary And Animal Sciences,

Durg, Chhattisgarh, India. 2012

Methionine supplemented groups were having higher egg weight

Basal diet Synthetic Methionine Herbal Methionine47

48

49

50

51

52

53

54

55

56

57

50.45

56.2556.13

Egg weight (g)

Higher egg mass was recorded with methionine supplementation

Basal

die

t

Synt

hetic

Met

hion

ine

Her

bal M

ethi

onin

e46

48

50

52 48.05

51.8 51.78Egg mass (g egg/ hen per day)

Avg. no .of eggs/ hen from 18-35 weeks

Group 1 (without DLM)

Group II (DLM) Group III (DLM + Methiorep)

84

86

88

90

92

94

96

98

100

102

90.55

101.15 100.84

Avg. No. of Eggs/Bird

A significant increase in number of eggs

produced per hen was evident in DL

Methionine group II and DLM +Methiorep

supplemented group III during experiemental

period.

Yolk Albumen Shell0

10

20

30

40

50

60

70

25.76

64.88

9.03

26.09

64.89

9.0227

64.9

9.02

Basal dietSynthetic MethionineHerbal Methionine

EGG COMPONENT PERCENTAGE

Basal diet Synthetic Methionine Herbal Methionine0

102030405060708090

42.28

78.37 80.7

Economics/Net Profit/bird (Rs.)

Herbal Methionine supplementation was found to be most economical….!!!

METHIOREPphytoadditive for replacement of synthetic methionine

BENEFITS

Natural analogue to replace synthetic methionine to cut your methionine supplementation

cost.

Economic & can give better performance of birds.

Powder form ensures equivalent distribution of the product in the feed.

Its activity remain unaltered during pelleting.

RECOMMENDED USAGE

BROILERS (1:1)

Methiorep: 1 part Methiorep and 1 part DL Methionine from day 1 to marketing age.

LAYERS (1:2)

Methiorep: 1 part Methiorep and 2 part DL Methionine from 1st week to 72nd week.

BREEDERS (1:3)

Methiorep: 1 part Methiorep and 3 part DL Methionine from 1st week to 72nd week.

I’d like another sack of the “METHIOREP” you sold me last week!!

This is an Ayurvet R&D presentation

Thank You!Questions invited!!