DEPARTMENT OF BIOCHEMISTRY

Introduction byMiss Sumaira khan

Senior lecturer

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FACULTY• Professor Dr Sajid Raza

MBBS,M-Phil

(Head of the Department biochemistry)

• Dr Sadia Haroon

MBBS,M-Phil (Associate Prof)

Dr Waseem kausar MBBS,M-Phil (Assistant Prof)

• Ms Sumaira Sarwar Khan

M.Sc, M-Phil

(Senior lecturer)

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Demonstrators

• Miss Maimoona (Msc Mphil Biochem)• Dr Zeeshan (MBBS) • Dr Mutassim (MBBS) • Dr Sajjad (MBBS)• Dr Adnan (MBBS)

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Introduction to Biochemistry

• Science concerned with chemical basis of life

Science concerned with the chemical constituents

of living cells and with the reactions and processes that they undergo

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Immunology

IT IS RELATED TO ALL OTHER SCIENCES VIZ;

Physiology

Pharmacology

Pathology

Microbiology

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Biochemistry studies have illuminated many aspects of health & disease

The study of various aspects of health & disease has opened up new areas of biochemistry

Normal biochemical processes are the basis of health

• The situation in which all of the many thousands of intra & extra cellular reactions that occur in the body are proceeding at the rates balanced with the individual’s maximal survival in the physiological state

A strict biochemistry viewpoint

about health:

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Biochemistry

Nucleic

acidprotein lipid Carbohydrates

Genetic

disease

Sickle cell anemia Medicine

arterioscler-osis

Diabetes mellitus

What are the goals of a medical biochemistry

course?• Learning and appreciating biochemistry; • course specific goals are the following:

– To understand how large molecules are synthesized and function (DNA, RNA, proteins)

– To understand how energy is stored, retrieved and generated (metabolism)

– To understand the future of diagnostic medicine (human genetics and cancer)

– To understand biological processes at the molecular level

ASSESMENT METHODS

• CLASS TESTS & VIVA VOCE

• TUTORIAL DISCUSSION

• PRESENTATIONS BY STUDENTS

• CHARTS PREPARATION

• ASSIGNMENTS

• BIOCHEMISTRY COURSE CONTENT • INTRODUCTION• BIOCHEMISTRY OF CELL• BIOLOGICAL MEMBRANES • BIOCHEMISTRY OF BODY FLUIDS• CHEMISTRY OF CARBOHYDRATES• PROTEINS• AMINO ACIDS• NUCLEOTIDES AND NUCLEIC ACIDS• LIPIDS • ENZYMES • PORPHYRINS AND HAEMOGLOBIN • VITAMINS • DIGESTIVE TRACT • MINERALS AND TRACE • ELEMENTS

•COURSE CONTENTS FOR PRACTICALS

•INTRODUCTION TO LAB FACILITIESRules to be observed while workingPrecautions and emergency first aid measures for working in medical laboratoryIntroduction of various techniques, equipmentsSolutions, definition and preparation of various types- of solutions

•PHYSICOCHEMICAL ASPECTSpH meteryDemonstration of effects of solutions of different- osmotic concentrations on RBCDemonstration of adsorptionObservation of effects of various compounds on- surface tension

•QUALITATIVE ANALYSIS OF CARBOHYDRATES

•Molisch's test•Iodine test for polysaccharides•Reducing action of sugars in alkaline medium•Benedicts test•Fehling's test•Barfoed's test. Reducing action of sugars in-•acidic medium•Detection of keto sugars•Selivanoff’s test•Hydrolysis of starch by mineral acids•Hydrolysis of sucrose by mineral acids•Scheme for detection of unknown CHO

•CHEMISTRY OF LIPIDSSolubility of fatsTest for identification of cholesterolEmulsification of fatsAcrolein formation

•CHEMISTRY OF PROTEINSColored reactionPrecipitation of protein by different reagents

Effect of strong acidsPrecipitation of proteins by salts of heavy- metalsPrecipitation of proteins by acid or alkali-reagents Precipitation by alcohol

Fractional precipitation of proteins with- ammonium sulphateScheme for detection of unknown proteins

•URINANALYSIS

Analysis of normal urine sampleAnalysis of abnormal urine sample

•ESTIMATION OF BLOOD GLUCOSE LEVEL•ESTIMATION OF HEMOGLOBIN

RECOMMENDED BOOKS

• HARPER’S ILLUSTRATED BIOCHEMISTRY

• LIPPINCOT’S REVIEW OF BIOCHEMISTRY

• JAYPEE’S BIOCHEMISTRY

• LEHNINGER PRINCIPLE’S OF BIOCHEMISTRY