Presentation of ALGEBAKT project.Antibacterial effect of arctic macroalgae

speciesDMV, PhD, Margarita Novoa-Garrido

Alta, Norway, Sept, 6th - 8th, 2010: The 7th  Circumpolar Agricultural Conference"Circumpolar Agricultural and Land Use Resources - Prospects and Perspectives for Circumpolar Productions and Industries" 

The MABIT-programme is an industrial R&D program for Northern Norway.

Bioforsk Nord Bodø. Bioproduction and ecosystems along the coast

Coastal ecology - possibilities for commercial exploitation of coastal and marine resources without damaging ecosystems. Our main focus is the algae.

• Antibiotics: therapy and growth promotion

• Ab-resistance• Antibiotics banned in Europe

as antibiotic growth promoters since 2006

Background for the project

Antibiotic resistance test of P. aeruginosa strain

It was on a short-cut through the hospital kitchens that Albert was first approched by a member of the Antibiotic Resistance.

Palmaria palmata

Pelvetia canaliculata Alaria esculenta

Foto: Unni Lorentsen

A. nodosum

ALGINATE

SEAWEED MEAL Sheep in seaweed meal project in

Bioforsk Nord.Foto: H.J. Skulstad

L. hyperboreaA. nodosum

Vegetables in seaweed project in Bioforsk Nord.Foto: I. Øvsthus

Algae composition

• Dietary fibre (<8% DM)• Protein (5-10 % DM)• Fat (2-4 % DM)• Carbohydrates (45-60 % DM)• Vitamines: A, C, E, B1, B2, B3, B8, B9 • Minerals• Secundary bioactive metabolites

Macroalgae biologiske effekter

• Antibakterial• Antivirus• Antitumor• Antioxidant• Antiinflammatory• Immune stimulans• Growth stimulant

Animal health

Diet

Environment

Bacterial population

Immune system

Jejunum and ileum104-106 CFU/mlLactobacilli, coliforms,Streptococci, fusobacteria, clostridia, bacteriodes, bifido bacteria

Stomach up to 102

Lactobacilli, streptococci, yeast

Colon 1010-1012

CFU/mlLactobacilli, bifido bacteria, eubacteria, fusobacteria, coliforms, clostridia, veillonella, yeasts

Photo: Birger Volden

Some research results

• Animal husbandry: nr. of E. coli intestine, shedding of E. coli O157 and Salmonella, nr. adherent Lactobacillus sp. Innate immunity and B lymhocyte activity.

• Aquaculture: Antibacterial effect on fish pathogens such as Vibrio sp. and Aeromonas sp.

• Furanones, organohalogens, floratannins, fucoidans, haloperoxidases, alginate

Aims of the project

• Generate knowledge about bioactive components with antibacterial effect in macroalgae species adapted to the northern Norwegian climate to get a better overview of the potential for developing new products based on their antibacterial property.

Material and methods

• Bacteria sheep: Lactobacillus sp., Enterococcus faecium, Enterococcus faecalis, different E. coli strains

• Bacteria fish:Vibrio sp., Pseudoalteromonas sp., Aeromonas sp.

• Macroalgae: Four red species and two brown species

• Minimum inhibitory concentration (MIN)• Liquid chromatography-mass spectrometry

Synergy with other ongoing prosjects• «Natural sources of antioxidants – a necessity

for animal health and welfare and product quality in organic livestock production.»

• The research Council of Norway.• Effekt of feeding macroalgae on the milk

quality of dairy cattle.• Effekt of feeding macroalgae on intestinal

bacterial flora and immune system of sheep.

Future visions

• Screening• Identification of biologically activ components• Animal experiments

THANK YOU FOR YOUR ATTENTION!