7/29/2019 Presences of Neurotoxin ODAP in Hedysarum Alpinum and Hedvsarum Mackenzii seeds

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WendyGruberIndependent esearchPresences f Neurotoxin ODAP in Hedysarumand Hedvsarum Mackenzii seeds

IntroductionThe goal of this research s to determine if OD

Diaminopropionicacid) or BOAA (p-N-Oxalylamino-L-aLathynrsspecies f HedysarumAlpinum and HedysarummaODAP could be causing heseseeds o be toxic. ODAP is a

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The next hing I researchedas he separation f amimethods. combined neof Rao'sseparationechniques ith ain Basic BiochemicalMethods. I ran two separate els hatacids and compared hese o our standardODAP sample. Icommonaminoacid hatappearedt hesame potasODAP o

I looked or differentmeanso separatehe seedsanODAP. During his processwith the mechanismshadavailalook to be thebestoption. Therewere wo differentmethods

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Hedysarum lpinumandHedysarummackenzii.This s controQuereshi,estedhe species f Hedysarumhat I tested.It apppurificationof ODAP from the LathynrsSativusseedss notHarrison,Nunn,andHill methodn my analysis. n the ODAPto make t more soluble.So in my Lathynrssativussamplepreparationso see f it offeredany differencen Rfvalues. Tois definitelypresentn the seedswe would need o u$e anotprobablyby HPLC-MSso we could ook at the actualmassof

7/29/2019 Presences of Neurotoxin ODAP in Hedysarum Alpinum and Hedvsarum Mackenzii seeds

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7/29/2019 Presences of Neurotoxin ODAP in Hedysarum Alpinum and Hedvsarum Mackenzii seeds

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