PREPARATION OF A BACTERIAL SMEARPREPARATION OF A BACTERIAL SMEAR

SPECIAL STAINING TECHNIQUESSPECIAL STAINING TECHNIQUES

ASEPTIC PROCECURE FOR MICROBE REMOVAL

http://www.youtube.com/watch?v=0odxJy0nR9s&feature=related

ASEPTIC PROCECURE FOR MICROBE REMOVAL FOR PREPARATION OF A BACTERIAL SMEAR

http://www.youtube.com/watch?v=HxjN2mBLGCE&feature=related

http://www.youtube.com/watch?v=n-HHI4E1LhI&feature=related

SPECIMEN PREPARATION FOR LIGHT MICROSCOPY PREPARATION OF A BACTERIAL SMEAR

STAINING SPECIAL STAINING TECHNIQUES

Negative Staining for Capsules» Capsules

• Determinants for virulence in some genera of bacteria

» Capsule Staining• Preparation of a bacterial smear along with

negative staining w/ Nigrosin or India Ink• Counterstain w/ Crystal Violet

STAINING SPECIAL STAINING TECHNIQUES

Negative Staining for Capsules» Preparing a bacterial smear for Capsule Staining

STAINING SPECIAL STAINING TECHNIQUES

Negative Staining for Capsules» Capsule Stain Results

STAINING SPECIAL STAINING TECHNIQUES

Endospore Stain Genera of bacteria that produce endospores

» Bacillus & Clostridium» Other genera: Desulfotomaculum, Sporosarcina,

Sporolactobacillus, Oscillospira, Thermoactinomyces» Schaeffer-Fulton Method for Endospore Staining

• Preparation of a Bacterial Smear-Air dry• Application of Primary Dye (Malachite Green) w/

concurrent application of heat• Allow slide to cool• Rinse w/ water• Application of Counterstain (Safranin)

http://www.tgw1916.net/video_pages/Spore.html

http://www.answers.com/topic/endospore

STAINING SPECIAL STAINING TECHNIQUES

Endospore Stain Results

Flagellar Stain» Uses mordant and carbolfuchsin stain to increase

flagellar diameter until resolved by light microscope

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http://www.answers.com/topic/endospore

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