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http://www.hysitron.com/Products/products.htm
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A)B)
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Johnson, J. E, et. al., J. of Ultastructure Research 1981, 74,
223-231.White, J. M.; Johnson, J. E., Virology 1980, 101,
319-324.A) Body Centered Cubic CPMV(100)B) Hexagonal CPMV (0001)2-8
nm - ununiform pores30 nm30 nm60 nm
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Varying temperatureVarying NaClA)B)
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Varying temperatureVarying NaClVarying pH
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MorphologyX-ray DiffractionA)
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pH = 6.0pH = 5.5pH = 5.0LiteratureWang, Q., ,et. al., Angewandte
Chemie-International Edition, 2002. 41(3): p. 459-462.A)B)C)D)
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Summary of the nanomechanical tests results for cross-linked
lysozyme crystalsB) Multiple load-displacement plots of cystals
cross-linked in 10% glutarladehyde for 24 hours A) The hardness of
protein crystal as a function of cross-linking degree by Hysitron
triboindenter.
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Pd distribution by SEMCross-linked protein crystalsSoaked
protein crystals with K2PdCl4A)B)C)D)E)
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S2p in methionine 162.59 eVS2p in cysteine disulfides 166.88
eVPd3d in K2PdCl4 338.2 eVPd bound to sulfur containing amino
acidsA)B)C)D)
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B) Cd(II) adsorption isotherm on two sized HEWL crystals 6 m
crystals500 m crystalsA) Cd(II) adsorption comparison with
ICP-AES
Chart1
0144.6
838.998546169400.6
2095.2104644269842.2
3444.1174111969
3 m crystal
500 m crystal
Cd (ug/L)
q(ug/kg)
Sheet1
Cd addedCd in Solution (ug/L)Cd added (ug/g protein)
000
21.2770.4
52.9971
105.7892
Cd addedCd measuredCd added(ug/g protein)
(ppb)(ppb)
Blank #10.023
Blank #20.01
Blank #30
2 ppb1.2770.4
5 ppb2.9971
10 ppb5.7892
Sheet1
0
0
0
0
Cd added (ppb)
Cd in solution (ppb)
Cadmium adsorption on nano protein
Sheet2
0
0
0
0
Cd Conc added (ug/g protein)
Cd Conc. in solution
Cd adsorption on nano protein
Sheet3
Protein , 3nm
Cd added (ug/L)Cd in Solution (ug/L)Cd in Protein(ug/kg)Cd in
Solution (ug/L)Cd added (ug/g protein)
21.277144.600
52.997400.61.2770.4
105.789842.22.9971
5.7892
Sheet3
0
0
0
Cd Conc. in solution (ug/L)
Cd Conc. on protein (ug/kg)
Cd adsorption on nano protein
Protein 3um
Stock Cd (ppb)1000
Wt. Bottle(g)Wt. Protein (g)Total Vol (g)Cdadded Vol(ml)Initial
Cd ICP-MS(ug/L)ICP-MS(ug/L)q(ug/Kg)Cd added(ug/L)Cd in
solution(ug/L)q (ug/kg)
No Cd10.2970.047920.137600-0.0050
110.17290.050920.03050.27.7575.625838.9985461692
ppb1.277144.6
210.17950.050620.25170.519.90514.672095.21046442695
ppb2.997400.6
310.2020.051820.0163138.28429.3713444.117411196910
ppb5.789842.2
00
00
00
0
Protein 3um
large protein
Cd (ug/L)
q(ug/kg)
q(ug/Kg) vs Conc for adsorptiion of Cadmium to protein at 8
pH
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Conversion (%)Yield (%)No
Pd6819K2PdCl48339PdCl2(PhCN)28449Native
CLPC95483.8%Pd/CLPC95716.5%Pd/CLPC95767.5%Pd/CLPC95708.5%Pd/CLPC9368Silica/Pd7220
