THE DELIVERY EXPERTS

TransfectionReagents

2014

DNA &/or siRNA Transfection Reagent

jetPRIME®

Use only one highly efficient and multipurpose reagent

jetPRIME® is the latest generation transfection reagent designed by Polyplus for effi cient delivery of DNA and siRNA into many adherent cell types.

jetPRIME® provides higher transfection effi ciency, uses less reagent, is cost effective, and is gentle on cells.

• Superior DNA transfection efficiencyYields higher transgene expression

• Easy protocol, compatible with serum & antibioticsSave time, no media changes prior transfection and no need for Opti-MEM®

• Recommended for virus production in HEK-293T & VERO cells in serum-containing medium

Obtain high viral titers using less DNASuitable for cotransfection of multiple plasmids

• Versatile: DNA, siRNA and cotransfectionsKeep only one reagent in the fridge

• siRNA TransfectionAchieve over 90% gene silencing at 10 nM siRNA

• Cost-effectiveUse less reagent, less DNA and perform 2-3 times more experiments per vial

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HeLa Cos-7 NIH-3T3 CHO-K1 HEK-293

% Tr

ansf

ectio

n ef

ficie

ncy jetPRIME® L2K

FACS analysis 24 h after GFP plasmid transfection according to the manufacturer’s recommendation.

• Better cell viabilityMore reliable and more reproducible results

Phase contrast microscopy of HeLa cells 24 h after transfection according to the manufacturer’s recommendation.Ph f H ll 224 h f f d h2

jetPRIME® easy protocol for 6-well plates.

¢¢

Dilute DNA/siRNA in jetPRIME® buffer

Add jetPRIME®

Vortex 10 s and Incubate 10 min at RT

Add transfection mix

Incubate 24 to 72 h and measure gene or protein expression

jetPRIME Reagent L2K

Search for transfection conditionsCell Transfection Database

6-well plate

Reagent Volume of reagentper well

Amount of DNAper well

Number of transfectionsper 1.5 ml vial

jetPRIME® 4 μl 2 μg 375L2K 10 μl 4 μg 150

siRNA Transfection Reagent

INTERFERin®

Transfect at low siRNA concentration and avoid off-target effects

In Vivo DNA & siRNA Delivery Reagent

in vivo-jetPEI®

The nonviral solution

- INTERFERin®-HTS for siRNA

- jetPEI® for DNA

High Throughput Screening Transfection Reagents

INTERFERin® is a new generation siRNA transfection reagent which provides more than 90% silencing effi ciency at 1 nM siRNA in a wide variety of cells.

in vivo-jetPEI® is the delivery reagent of choice for functional studies and RNA interference experiments in vivo. This linear polyethylenimine derivative is also well-suited as a delivery vehicle for therapeutic goals, including viral diseases and cancer.

• Successful in vivo delivery of DNA, siRNA and oligos

• Currently used in phase I and II clinical trials

• Multiple modes of administration in many species

• No inflammatory response

• Exceptionally efficient and reproducible

• Nucleic acid/reagent complexes stable up to 4 hours

• Specific protocols for automated liquid handling devices

Selected successfully transfected cell lines and silencing effi ciencies obtained with INTERFERin®.

Effi cient and specifi c gene silencing following 1 nM INTERFERin-mediated siRNA transfection. Immunofl uorescence microscopy 48h after transfection

Successful administration routes in mouse.

Adherent cell lines (1 nM siRNA)

A549

> 90%

HeLa

CaSki

MCF7

NIH-3T3

RAW 264.7

SiHa

HepG2 60-70%Primary cells (1 nM siRNA)

Murine embryonic fi broblasts

> 90%Primary human fi broblasts

Primary human hepatocytes

Suspension cell lines (5 nM siRNA)

K562> 80%

THP-1

INTELLECTUAL PROPERTYThe use of any polyethylenimine (PEI) or polypropylenimine (PPI) or cationic polymers similar in structure thereto for transfecting cells, as well as compositions comprising these cationic polymers and at least one nucleic acid, are the subject matter of U.S. Patent No. 6,013,240, EP Patent No. 0770140 and foreign equivalents, for which Polyplus-transfection® is the worldwide exclusive licensee.

Product Citations Extensive list of publications available

• Work at 1 nM siRNAAvoid off-target effects and get high gene silencing

• Easy protocol, compatible with serum & antibioticsSave time, no media changes prior transfection

• Excellent cell viabilityTrust your results

• Up to 90% gene silencing in many cell types

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Check our other flyers for our Bioproduction specific reagents

Reagent Reagent size Buffersize

PolyplusCat. N°

jetPRIME® Transfection reagent + bufferBroad range of adherent cell lines, DNA and/or siRNA transfection

0.1 ml 5 ml 114-01

0.75 ml 60 ml 114-07

1.5 ml 2 x 60 ml 114-15

5 x 1.5 ml 10 x 60 ml 114-75

jetPRIME® Transfection reagent + buffer 5 x conc. 5 x 1.5 ml 120 ml 114-75C

INTERFERin® Transfection ReagentsiRNA day-to-day transfection

0.1 ml - 409-01

1 ml - 409-10

5 x 1 ml - 409-50

in vivo-jetPEI® Delivery Reagent + glucose sol.DNA and siRNA in vivo delivery

0.1 ml 10 ml 201-10G

0.5 ml 2 x 10 ml 201-50G

INTERFERin®-HTS Transfection ReagentsiRNA High Throughput Screening

0.2 ml - 410-002

1.5 ml - 410-015

4 x 1.5 ml - 410-060

jetPEI® Transfection Reagent + NaCl solutionDNA High Throughput Screening

0.1 ml 5 ml 101-01N

1 ml 50 ml 101-10N

4 x 1 ml 4 x 50 ml 101-40N

10 ml 2 x 250 ml 101B-010N

jetPEI® DNA Transfection Reagent DNA High Throughput Screening

1 ml - 101-10

4 x 1 ml - 101-40

10 ml - 101B-010

PEIpro™ DNA Transfection ReagentProtein & virus production

10 ml - 115-010

375 ml - 115-375

jetPEI®-Hepatocyte Transfection Reagent + NaCl solutionDNA transfection into hepatocyte cells

0.1 ml 5 ml 102-01N

0.5 ml 50 ml 102-05N

jetPEI®-Macrophage Transfection Reagent + NaCl solutionDNA transfection into macrophage cells

0.1 ml 5 ml 103-01N

0.5 ml 50 ml 103-05N

jetPEI®-HUVEC Transfection Reagent + NaCl solutionDNA transfection into HUVEC

0.1 ml 5 ml 108-01N

0.5 ml 50 ml 108-05N

jetPEI®-FluoF Transfection Reagent + NaCl solution, Fluorescent green labeling 0.5 ml 50 ml 105-05N

jetPEI®-FluoR Transfection Reagent + NaCl solution, Fluorescent red labeling 0.5 ml 50 ml 106-05N

PULSin® Protein, Antibody and Peptide Delivery ReagentHepes solution + 200 μL positive control included

0.1 ml 20 ml 501-01

0.4 ml 20 ml 501-04

jetSI® 10 mM Delivery Reagent, siRNA delivery into the brain 0.5 ml - 403-05

in vivo-jetPEI®-Gal Delivery Reagent + glucose sol., Galactose-conjugated 0.1 ml 10 ml 202-10G

in vivo-jetPEI®-Man Delivery Reagent + glucose sol., Mannose-conjugated 0.1 ml 10 ml 203-10G

in vivo-jetPEI®-FluoF Delivery Reagent + glucose sol., Fluorescent green labeling 0.1 ml 10 ml 205-10G

in vivo-jetPEI®-FluoR Delivery Reagent + glucose sol., Fluorescent red labeling 0.1 ml 10 ml 206-10G

(1) J.-P. Behr, B. Demeneix, J.-P. Loeffl er, J. Perez-Mutul, "Effi cient gene transfer into mammalian primary endocrine cells with lipopolyamine coated DNA", Proc. Natl. Acad. Sci. USA, 86, 6982, 1989.

(2) O. Boussif, F. Lezoualc'h, M.A. Zanta, M.D. Mergny, D. Scherman, B. Demeneix, J.P. Behr, "A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine." Proc. Natl. Acad. Sci. USA, 92, 7297-7301, 1995.

Prices and availability are subject to change. Copyright© 2014 Polyplus-transfec on SA. All Rights Reserved. Each product is sold with a limited warranty which is provided with each purchase. Each product is intended to be used for research purposes only. It is not to be used for drug or diagnos c purposes nor is it intended for human use. Polyplus-transfec on SA products may not be resold, modifi ed for resale, or used to manufacture commercial products without wri en approval of Polyplus-transfec on SA. INTERFERin, jetPEI, jetPRIME, jetSI, PEIpro, Polyplus-transfec on and PULSin are registered trademarks of Polyplus-transfec on SA. Op -MEM is a registered trademark of Life Technologies.

Polyplus-transfection started in 2001 to market transfection products developed in the lab of Dr. Jean-Paul Behr, who is considered one of the preeminent transfection researchers in the world. Seminal work was published in 1989 on the use of polycationic lipids for the use of transfection (1) that led to several still popular transfection reagents. The next advancement in this fi eld is associated with Polyethylenimine (PEI), a cationic polymer. PEI was discovered as an improved transfection reagent in 1995 (2) and its associated «proton sponge» transfection paradigm is at the root of many modern transfection reagents.

Polyplus-transfection continues its strong tradition of scientifi c excellence and innovation offering the best reagents for delivery of nucleic acids in the market today.

THE DELIVERY EXPERTS