Keeling et al.

MMETSP

project PLOS

Bio 2014

Chrysochromulina tobin

Environmental

Sample Antibiotic

treatments

Reiterative

flow cytometry

Sequencing

Cleaned

Culture

Lab

conditions

Blast2GO

•Genomes:

•Mitochondrial

•Chloroplast

•Nuclear

•Transcriptomes:

•7 time points over photoperiod

•Proteomes

•Whole cell

•Lipid body isolation

KEGG database

“Omes” competed:

High throughput

sequencing (Illumina

and 454)

Assembly

Lipid pathway from

Radakovits et. al. 2010

Bigelow et. al. (Algal Research, 2013)

• DHA

• EPA

• Stearic

• Palmitic

• Myristic

• …etc…

Total lipid ~17mg/L

Hovde et. al. (in final preparation)

Acknowledgements

Monnat Lab (UW)

•Ray Monnat

•Ryan Sinit

Cattolico Lab (UW)

•Rose Ann Cattolico

•Chloe Deodato

•Heather Huntsperger

•Will Yost

•John Patterson

•Stephanie Brunelle

•Bill Hardin

Los Alamos Natl Lab

•Shannon Johnson

•Shawn Starkenburg

Rocap Lab (UW)

•Gabrielle Rocap

•Cedar McKay

Funding and support:

Pacific Northwest Natl.

Lab

•Mary Lipton