POSTERS Last update: 10th February 2013 I: Preclinical Changes in Immune-mediated Inflammatory Disease

1.1 ANTI-CITRULLINATED PROTEIN ANTIBODY SPECIFIC FC GLYCOSYLATION PATTERNS IN ARTHRALGIA PATIENTS Kempers AC1, Scherer HU1, Rombouts Y1, Ewing E1, van de Stadt LA2, Selman MHJ3, Deelder AM3, Huizinga TWJ1, Wuhrer M3, van Schaardenburg D2, Toes REM1 1 Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands; 2 Department of Rheumatology, Jan van Breemen Research Institute/Reade, Amsterdam, The Netherlands; 3 Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands

1.2 FIBROBLASTS INFLUENCE LYMPHOCYTE RECRUITMENT AND MIGRATION DURING RESOLVING AND PERSISTENT ARTHRITIS. Helen M. McGettrick (1,3), Andrew Filer (1,3), Karim Raza (1,3), Gerard B. Nash (2) and Christopher D. Buckley (1,3) (1)Centre for Excellence in the Pathogenesis of Rheumatoid Arthritis and (2) Centre for Cardiovascular Sciences, College of Medical and Dental Sciences and (3) Systems Science for Health, University of Birmingham, Birmingham, B15 2TT, UK

1.3 CITRULLINATION IN HEALTHY AND INFLAMED LUNG TISSUE AS A PRIMING SITE FOR AUTOIMMUNITY IN RA Elena B. Lugli1, Anna Montgomery1, Karin Lundberg2, Ken Bracke3, Guy Brusselle3 and Patrick J. Venables1 1Kennedy Institute of Rheumatology, Nuffield Department of Orthopaedics, Rheumatology and Muscular Diseases, University of Oxford, Uk; 2Karolinska Institutet, Stockholm, Sweden; 3Ghent University, Gent, Belgium.

1.4 EARLY SIGNS OF SUBCLINICAL INFLAMMATION AND LOCAL ANTIBODY PRODUCTION IN EARLY RHEUMATOID LUNGS Gudrun Reynisdottir1, Reza Karimi2, Helga Olsen2, Vijay Joshua1, Aase Haj Hensvold1, Anders Harju1, Marianne Engström1, Johan Grunewald2, Sven Nyren3, Anders Eklund2, Lars Klareskog1, Magnus Sköld2, Anca Irinel Catrina1* 1Unit of Rheumatology, Department of Medicine, Karolinska University Hospital, Karolinska Institutet, Stockholm, Sweden; 2Division of Respiratory Medicine, Department of Medicine, Karolinska Institutet, Karolinska University Hospital Solna, Stockholm, Sweden; 3Department of Molecular Medicine and Surgery, Karolinska Institutet, Karolinska University Hospital Solna, Stockholm

1.5 EXPLORING THE ROLE OF THE LYMPH NODE MICROENVIRONMENT IN HEALTH AND DISEASE J. Hähnlein1,2, T.H. Ramwadhdoebe 1,2, K.I. Maijer1, Y.K. Choi1, N.A.M. Smits1,2, M. Maas3, D.M. Gerlag1, P.P. Tak1, L.G.M. van Baarsen1,2 1Division of Clinical Immunology & Rheumatology, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands; 2Division of Experimental Immmunology, Academic

Medical Center, University of Amsterdam, Amsterdam, Netherlands; 3Division of Radiology, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands

1.6 IDO PATHWAY IN RA PATIENTS RESPONDING TO BIOLOGIC TREATMENTS G Falgarone1, 2, S Rullé1,2*, X Guillot 1,2*, L Semerano1,2, C Laroche3, E Assier1,2, MC Boissier1,2 1 Sorbonne Paris Cité, Université Paris 13, EA4222, Li2P, 93000 Bobigny, France; 2 Assistance Publique-Hôpitaux de Paris (AP-HP), Groupe Hospitalier Nord, hôpital Avicenne, Service de Rhumatologie, 93009 Bobigny, France; 3 Assistance Publique-Hôpitaux de Paris (AP-HP), Groupe hospitalier Nord, hôpital Avicenne, Service de Médecine interne, 93009 Bobigny, France

1.7 INTERFERON AND B-CELL GENE SIGNATURES CONTRIBUTE TO DIAGNOSIS OF PRE-CLINICAL RHEUMATOID ARTHRITIS Joyce Lubbers1, Lotte A. van de Stadt2, Saskia Vosslamber1, John G. Wesseling1, Senay Oztürk1, Dirkjan van Schaardenburg2 and Cornelis L. Verweij1 1VU University Medical Center, Amsterdam, Netherlands, 2Jan van Breemen Research Institute | Reade, Amsterdam, Netherlands

1.8 MAGNETIC RESONANCE IMAGING OF HAND AND FOOT JOINTS OF PATIENTS WITH ACPA POSITIVE ARTHRALGIA WITHOUT CLINICAL ARTHRITIS A. Krabben, W. Stomp, D.M.F.M. van der Heijde, J.A.B. van Nies, J.L. Bloem, T.W.J. Huizinga, M. Reijnierse, A.H.M. van der Helm-van Mil Department of Rheumatology and Radiology, Leiden University Medical Center

1.9 PREDICTION OF TREATMENT RESPONSE BY ACPA LEVELS AMONG PATIENTS WITH RHEUMATOID ARTHRITIS Raquel Hernández, Paz Gonzalez, Julia Uceda, Jose Luis Marenco de la Fuente Valme University Hospital, Seville Presenting Author: Maria Louisa Velloso

II: Innate Immunity 2.1 ACTIVATED NEUTROPHILS ARE ABLE TO EFFICIENTLY PRODUCE INTERFERON-Α AND RETAIN

THIS CAPABILITY IN SYSTEMIC LUPUS ERYTHEMATOSUS AND RHEUMATOID ARTHRITIS PATIENTS Dennis Lindau1, Armin Rabsteyn1, Julie Mussard2, Matthieu Ribon2, Ina Kötter3, Gosse Adema4, Marie-Christophe Boissier2 and Patrice Decker2* 1University of Tübingen, Institute for Cell Biology, Department of Immunology, Tübingen, Germany, 2University of Paris 13, Sorbonne Paris Cité, EA4222, Li2P, Bobigny, France, 3University Hospital, Internal Medicine II, Tübingen, Germany, 4Nijmegen Centre for Molecular Life Sciences, Department of Tumor Immunology, Nijmegen, The Netherlands.

2.2 ACUTE SERUM AMYLOID A (A-SSA) INDUCES TIMP-1 IN DERMAL FIBROBLASTS THAT IS MEDIATED VIA A TLR-DEPENDANT AND FORMYL RECEPTOR-INDEPENDENT PATHWAY Steven O'Reilly, Rachel Cant, Marzena Ciechomska, Jacob M van Laar MRG, Institute of Cellular Medicine, Newcastle University

2.3 ANTI-APOPTOTIC IGG ANTIBODIES FROM PATIENTS WITH PRIMARY SJOGREN’S SYNDROME AND SYSTEMIC LUPUS ERYTHEMATOSUS INHIBIT THE PHAGOCYTOSIS OF APOPTOTIC CELLS. Aigli G. Vakrakou, MD, George E. Fragoulis, MD, Menelaos N. Manoussakis, MD, PhD. Dpt Pathophysiology, University of Athens, Greece.

2.4 ASSOCIATION OF CD55 WITH RETICULAR FIBERS IN THE SYNOVIAL LINING IN RHEUMATOID ARTHRITIS ON Karpus1, B Niederreiter2, PP Tak2, JS Smolen2, HP Kiener2, J Hamann1 1Department of Experimental Immunology, Academic Medical Center, Amsterdam, The

Netherlands and 2Department of Medicine III, Division of Rheumatology, Medical University of Vienna, Vienna General Hospital, Vienna, Austria

2.5 CD11C+ DENDRITIC CELLS PLAY AN IMPORTANT PROINFLAMMATORY ROLE IN INFLAMMATORY ARTHRITIS A.Puchner, B. S. Blüml, V. Saferding, B. Niederreiter, H. Leiss, J.S. Smolen, K. Redlich Medical University of Vienna

2.6 CELL-SPECIFIC TYPE I IFN SIGNATURES IN AUTOIMMUNITY AND VIRAL INFECTION: WHAT MAKES THE DIFFERENCE? Chieko Kyogoku1, Biljana Smiljanovic1, Joachim R Grün1, Tobias Alexander2, Robert Biesen2, Falk Hiepe2, Thomas Häupl2, Andreas Radbruch1, Andreas Grützkau1 1German Rheumatism Research Centre (DRFZ) Berlin, An Institute of the Leibniz Association, Berlin, Germany; 2Department of Rheumatology and Clinical Immunology, Charité University Medicine Berlin, Humboldt University of Berlin, Berlin, Germany

2.7 EFFECTS OF VAGUS NERVE STIMULATION ON THE CENTRAL PROSTAGLANDIN SYSTEM AND SUBSTANCE P FOLLOWING PERIPHERAL INFLAMMATION Priya Revathikumar, Erwan Le Maître, Per-Johan Jakobsson, Marina Korotkova, Jon Lampa Department of Medicine, Rheumatology Unit, Center for Molecular Medicine (CMM), Karolinska Institutet, Stockholm,

2.8 ENHANCED NEUTROPHIL EXTRACELLULAR TRAP FORMATION IN RHEUMATOID ARTHRITIS PATIENTS IS CORRELATED WITH HIGH LEVELS OF RHEUMATOID FACTOR (RF) Garyfalia Papadaki1, Christiana Choulaki1, George Bertsias1, Ioannis Mitroulis2, Panayotis Verginis3, Argyro Repa4, Amalia Raptopoulou4, Dimitrios Boumpas5, Prodromos Sidiropoulos1,4 1Laboratory of Autoimmunity and Inflammation, University of Crete, Medical School, Heraklion, Greece, 2First Department of Internal Medicine, Democritus University of Thrace, Alexandroupolis, Greece, 3Division of Immunobiology, Biomedical Research Foundation Academy of Athens, Athens, 4Department of Rheumatology, University of Crete, Medical School, Heraklion, Greece, 5Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, 71300, Heraklion, Greece

2.9 HIGH SERUM-CHOLESTEROL LEVELS BY EITHER LOW DENSITY LIPOPROTEIN RECEPTOR DEFICIENCY OR A CHOLESTEROL-RICH DIET RESULTS IN SYNOVIAL ACTIVATION AND OSTEOPHYTE FORMATION DURING EXPERIMENTAL OSTEOARTHRITIS W. de Munter, A. W. Slöetjes, B. Walgreen, M. M. Helsen, W. B. van den Berg, P. L. van Lent Department of Experimental Rheumatology & Advanced Therapeutics, Radboud University Nijmegen Medical Centre

2.10 INCREASED IMMUNOLOGIC EXPOSURE TO NECROTIC CELL REMNANTS IN PATIENTS WITH PRIMARY SJOGRENÍS SYNDROME OWING TO DEFECTIVE DNASE-I ACTIVITY AND THE PRESENCE OF OPSONIZING IGG AUTOANTIBODIES IN SERUM. Aigli G. Vakrakou, MD, George Ε. Fragoulis, MD, Menelaos N. Manoussakis, MD, PhD. Dpt Pathophysiology, University of Athens, Greece.

2.11 INVOLVEMENT OF THE NUCLEIC ACID RECOGNIZING TOLL-LIKE RECEPTORS TLR7 AND TLR9 IN THE PATHOGENESIS OF EROSIVE ARTHRITIS Anita Fischer*, Christina Böhm*, Victoria Saferding*, Eliana Goncalves-Alves*, Marije Koenders†, Wim van den Berg†, * Sonja Herman* and Günter Steiner * Division of Rheumatology, Internal Medicine III, Medical University of Vienna, Austria; † Department of Rheumatology, Radboud University Nijmegen Medical Centre, Netherlands

2.12 PHENOTYPE CHANGES OF BLOOD EOSINOPHILS REFLECT ACTIVITY AND SEVERITY IN SYSTEMIC SCLEROSIS Dirk M. Wuttge, Annika Andreasson, Ellen Tufvesson, Agneta Scheja, Roger Hesselstrand,Lennart Truedsson

Lund University, Department of Clinical Sciences, Lund, Section of Rheumatology, S-221 85 Lund, Sweden

2.13 PHENOTYPIC AND MOLECULAR PROFILE OF INNATE LYMPHOID CELLS IN CHRONIC SYNOVIAL INFLAMMATION Hulda Sigridur Hreggvidsdottir1,2, Jenny Mjösberg2, Jochem Bernink2, Dominique Baeten1, Hergen Spits2 1) Department of Clinical Immunology and Rheumatology, 2) Tytgat Institute for Liver and Intestinal Research, Academic Medical Center/University of Amsterdam, Amsterdam

2.14 POTENTIAL IN VITRO IMMUNOMODULATORY EFFECTS OF THE RECOMBINANT HUMAN ALPHA-ENOLASE ON PERIPHERAL BLOOD MONONUCLEAR CELLS (PBMCS) FROM HEALTHY DONORS. C. Guillou1, G. Avenel2, C. Derambure1, M. Verdet2, ML. Golinski1, M. Hiron1, S. Adriouch1, O. Boyer1, T. Lequerré2, O Vittecoq2 1INSERM U905, Institute for Biomedical Research, University of Rouen, Rouen, France 2Department of Rheumatology, Rouen University Hospital and INSERM U905, Institute for Biomedical Research, University of Rouen, Rouen, France 3Department of Immunology, Rouen University Hospital and INSERM U905, Institute for Biomedical Research, University of Rouen, Rouen, France

2.15 RELATIVE OVEREXPRESSION OF TRANSMEMBRANE VERSUS SOLUBLE TNF IN HUMAN AND EXPERIMENTAL SPONDYLOARTHRITIS Leonie M. van Duivenvoorde, Carmen A. Ambarus, Huriatul Masdar, Melissa N. van Tok, Paul P. Tak, Nataliya G. Yeremenko, Dominique L. Baeten Department of Clinical Immunology and Rheumatology and Department of Experimental Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.

2.16 SYNOVIAL FLUID NEUTROPHILS UNDERGOING NETOSIS CONTRIBUTE TO JOINT INFLAMMATION BY PRODUCING CITRULLINATED AUTOANTIGENS. Julia Spengler1, Bozo Lugonja1, Andrew Creese2, Anthony Nicholas3, Mike Milward2, Mark Pearson1, Christopher D. Buckley1, Andrew Filer1, Karim Raza1, Paul R Cooper2, Iain LC Chapple2 and Dagmar Scheel-Toellner1 1Centre for Translational Inflammation Research, School of Immunity & Infection, College of Medical & Dental Sciences, University of Birmingham Research Laboratories, Queen Elizabeth Hospital , Birmingham, B15 2WD; 2University of Birmingham, School of Dentistry, St. Chads Queensway, Birmingham, B4 6NN, UK; 3University of Alabama at Birmingham, Birmingham, AL 35294, USA

2.17 SYNOVIOCYTES CHANGE PHENOTYPE AND FUNCTION AFTER TREG-DEPLETION IN ARTHRITIC MICE. M. Böttcher1, I.Irmler1, C. Wunrau2, A.Korb-Pap2, G.Schett3, T. Pap2, T. Kamradt1. 1 University Hospital Jena, Institute of Immunology, Jena, Germany. 2 University Hospital Muenster, Institute of Experimental Musculoskeletal Medicine, Muenster, Germany. 3. University Hospital Erlangen, Department of Internal Medicine-3 and Institute for Clinical Immunology, Erlangen, Germany

2.18 THE EXPRESSION OF THE TRANSCRIPTION FACTOR PPAR-GAMMA IS SIGNIFICANTLY REDUCED IN THE SALIVARY GLAND EPITHELIAL CELLS FROM PATIENTS WITH PRIMARY SJOGREN’S SYNDROME. Aigli G. Vakrakou, MD, Vicky Gourzi, MD, Efstathia Kapsogeorgou, PhD, Menelaos N. Manoussakis, MD, PhD. Dpt Pathophysiology, University of Athens, Greece

2.19 THE ROLE OF MICRORNA 155 IN INNATE IMMUNITY AND ARTHRITIS Victoria Saferding, Eliana Goncalves-Alves, Antonia Puchner, Josef Smolen, Kurt Redlich,

Stephan Blüml Medical University Vienna, Dpt. of Rheumatology

2.20 THE ROLE OF SERUM FACTORS AND TOLL-LIKE RECEPTOR SIGNALING IN THE INDUCTION OF PROFIBROTIC TIMP-1 BY MONOCYTES IN SYSTEMIC SCLEROSIS Marzena Ciechomska1, Christiaan A. Huigens1, Thomas Hügle2, Bridget Griffiths3, Timothy R.D.J. Radstake4, Sophie Hambleton1, Steven O’Reilly1, Jacob M. van Laar1. 1Musculoskeletal Research Group, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK; 2Dept of Rheumatology, University Hospital Basel, Switzerland, 3Dept of Rheumatology, Freeman Hospital, Newcastle upon Tyne, UK; 4Dept of Rheumatology and Clinical Immunology, University Medical Centre Utrecht, The Netherlands.

2.21 TOLL-LIKE RECEPTOR DEPENDENT AUTOANTIGENS AND VESICLES FROM P.GINGIVALIS IN ANIMAL MODELS OF RA TO MODULATE COLLAGEN AND COLLAGEN ANTIBODY INDUCED ARTHRITIS B. Marklein2, M. Sohn2, Z.Konthur1, K Grimm2, G. Steiner3, F. Apparailly 4, G-R Burmester2, K. Skriner 1,2. 1Max-Planck-Institute for Molecular Genetics, Berlin, Germany; 2Charité University Medicine, Department of Rheumatology and Clinical Immunology, Humboldt University and Free University, Berlin, Germany. 3University Departments of the Vienna Biocenter. Department of Medical Biochemistry Dr. Bohrgasse 9/4, A-1030 Vienna, Austria. 4 INSERM, U844, Hôpital Saint Eloi, and Université Montpellier I, Montpellier, France

2.22 TYROSINE PHOSPHORYLATION PATHWAYS IN MYELOID CELL-MEDIATED INFLAMMATORY DISEASES Kovács M, Németh T, Futosi K, Jakus Z, Sitaru C and Mócsai A Department of Physiology, Semmelweis University School of Medicine, Budapest, Hungary; Department of Dermatology, University of Freiburg, Germany

2.23 IMPAIRED NATURAL KILLER CELL FUNCTION IN DOCK8 DEFICIENCY M.C. Mizesko1,2, P.P. Banerjee1,2, L. Monaco-Shawver3, E.M. Mace1,2, W. Bernal3, J. Sawalle-Belohradsky4, B. Belohradsky4, V. Heinz4, A.F. Freeman5, K.E. Sullivan, MD, PhD3, S.M. Holland5, T.R. Torgerson6, W. Al-Herz7, J. Chou8, I.C. Hanson1,2, M.H. Albert4, R.S. Geha8, E.D. Renner4, J.S. Orange1,2

1Baylor College of Medicine, 2Texas Children's Hospital, Houston, TX, 3Children's Hospital of Philadelphia Research Institute, Philadelphia, PA, USA, 4University Children's Hospital, Ludwig Maximilian University, Munich, Germany, 5National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA, 6University of Washington and Seattle Children's Hospital, Seattle, WA, USA, 7Kuwait University and Allergy and Clinical Immunology Unit, Kuwait City, Kuwait, 8Boston Children's Hospital, Boston, MA, USA

2.24 IL23/TH17-MEDIATED REGULATION OF ANTIBODY GLYCOSYLATION CONTROLS AUTOIMMUNE-INDUCED ARTHRITIS René Pfeifle1, 2, Iryna Magorivska1, Ulrich Scherer4, Ulrike Harre1, Tobias Rothe1, 2, Sybille Böhm3, Martin Herrmann1, Stephan Blüml, Falk Nimmerjahn3, Georg Schett1 and Gerhard Krönke1,2

(1)Department of internal Medicine 3 and Institute of Immunology, University Erlangen Nuernberg, (2)Nikolaus-Fibienger Center of Molecular Medicine, University Hospital and University of Erlangen Nuernberg (3)Department of Biology, University of Erlangen-Nuernberg, (4)Department of Rheumatology Leiden University Medical Center, (5)University Hospital, Department of internal Medicine 3 for Rheumatology, University of Vienna

III: T cells – Activation and Regulation

3.1 1,25(OH)2D3 INHIBITS TH17 POLARIZATION AND RORGAMMAT EXPRESSION THROUGH GATA3-DEPENDENT AND -INDEPENDENT MECHANISMS W. Dankers1,2, J.P. van Hamburg1,2, A.M.C. Mus1,2, P.S. Asmawidjaja1,2, O.B.J. Corneth1,2, F. Luk1,2, J.P.T.M. van Leeuwen3, R.W. Hendriks4, L. Boon5, E.M. Colin1,6, E. Lubberts1,2

Departments of 1Rheumatology, 2Immunology, 3Internal Medicine and 4Pulmonary Medicine, Erasmus MC University Medical Center, Rotterdam, The Netherlands, 5Bioceros, Utrecht, The Netherlands and 6Department of Rheumatology, ZGT, Almelo, The Netherlands

3.2 A CD4+ T-CELL GENE EXPRESSION SIGNATURE PREDICTS DRUG SURVIVAL ON METHOTREXATE MONOTHERAPY IN EARLY RHEUMATOID ARTHRITIS. Pratt AG1,2, Brown PM1, Cockell SJ3, Wilson G4, Isaacs JD1,2

1Institute of Cellular Medicine (Musculoskeletal Research Group), Newcastle University, UK; 2Bioinformatics Support Unit, Newcastle University, UK; 3Musculoskeletal Unit, The Freeman Hospital, Newcastle-upon-Tyne, UK; 4Faculty of Health and Social Care, University of Hull, UK

3.3 AN IMMUNOLOGICAL WINDOW OF OPPORTUNITY DEFINES THE ABILITY OF EARLY RA PATIENT TO ACHIEVE REMISSION WITH FIRST ANTI-RHEUMATIC TREATMENT. Agata Burska, Rekha Parmar, Yasser El-Sherbiny, Jehan El Jawhari, Paul Emery and Frederique Ponchel. University, Leeds, UK

3.4 CD3+CD4-

CD8- DOUBLE NEGATIVE TH17 CELLS: NEW INSIGHTS IN THE PATHOGENESIS OF PRIMARY SJÖGREN’S SYNDROME Alessia Alunno, Sara Caterbi, Onelia Bistoni, Elena Bartoloni, Gianluca Santoboni, Giulia Mirabelli, Francesca Cannarile, Valentina Valentini, Riccardo Terenzi, Roberto Gerli Rheumatology Unit, Department of Clinical and Experimental Medicine University of Perugia, Perugia, Italy

3.5 COMBINATION BLOCKING OF IL-6 AND IL-21 IN EXPERIMENTAL ARTHRITIS INHIBITS THEIR REDUNDANT ROLE IN TH17-DRIVEN JOINT PATHOLOGY Marije I. Koenders1, Debbie M. Roeleveld1, Renoud J. Marijnissen1,2, Cheryl Nickerson-Nutter3, Fons van de Loo1, Wim B. van den Berg1

1 Radboud University Nijmegen Medical Centre, Rheumatology Research and Advanced Therapeutics, Nijmegen, the Netherlands.; 2 University Medical Centre Utrecht, the Netherlands.; 3 Pfizer Inc, Cambridge, Massachusetts, USA

Presenting Author: Peter van Lent

3.6 COMPARATIVE ANALYSIS OF THE THERAPEUTIC POTENTIAL OF INDUCIBLE TREG CELL POPULATIONS IN EXPERIMENTAL MODEL OF ARTHRITIS Julie Quentin1, Delphine Martire1, Anne-Laure Mausset-Bonnefont1, Hélčne Asnagli2, Nathalie Belmonte2, Arnaud Foussat2, Christian Jorgensen1, Rita Vincente1 and Pascale Louis-Plence1. 1Inserm U844, Université Montpellier 1, CHU Lapeyronnie, Montpellier, France 2TxCell, Allée de la Nertičre, Les Cardoulines, 06 560 Valbonne-Sophia Antipolis, France Presenting Author: Florence Apparailly

3.7 COMPARISON OF THE EFFECTS OF TH17 AND TH1 CELLS ON ENDOTHELIAL CELLS AND

SYNOVIOCYTES Arnaud Hot1, Laura Maggi2 Fabien Lavocat1 Francesco Annunziato2 Pierre Miossec1

1Department of Clinical Immunology and Rheumatology; Immunogenomic and inflammation research unit EA 4130, Hopital Edouard Herriot, University of Lyon, France.; 2Department of Internal Medicine and DENOTHE Centre, University of Florence, Firenze, Italy.

3.8 EXTRATHYMIC AUTOIMMUNE REGULATOR (AIRE) EXPRESSION IN RHEUMATOID ARTHRITIS A.R. Noort, K.P.M. van Zoest1, M.C. Lebre, P.P. Tak and S.W. Tas Division of Clinical Immunology & Rheumatology, Academic Medical Center, University of Amsterdam, The Netherlands

3.9 IL-17RA SIGNALLING IS ESSENTIAL FOR COLLAGEN INDUCED ARTHRITIS DEVELOPMENT Odilia B.J. Corneth (1,2), Adriana M.C. Mus (1,2), Patrick S. Asmawidjaja (1,2), Maarten D. Brem (1,2), Franka Luk (1,2), Wendy Dankers (1,2), Rudi W. Hendriks (3), Erik Lubberts (1,2) Departments of (1) Rheumatology, (2) Immunology and (3) Pulmonary Medicine, Erasmus Medical Center, Rotterdam

3.10 IL-6 RECEPTOR BLOCKADE ENHANCES CD39+ REGULATORY T-CELL DEVELOPMENT IN RHEUMATOID ARTHRITIS AND IN EXPERIMENTAL ARTHRITIS. Allan Thiolat1, Jerome Biton1, Luca Semerano1, Yves-Marie Pers2, Pierre Portales2, Delphine Lemeiter1, Patrice Decker1, Christian Jorgensen2, Marie-Christophe Boissier1, Pascale Louis-Plence2, Natacha Bessis1 1EA4222, Li2P, University Paris 13, Sorbonne Paris Cité and Rheumatology Department, Avicenne Hospital, Assistance Publique-Hôpitaux de Paris (AP-HP), Bobigny, 93009, France, Bobigny, France, 2Inserm U844, CHU saint-Eloi, Université Montpellier 1, CHU Lapeyronie, Montpellier, France

3.11 IMMUNOSUPPRESSIVE EFFECTS OF GLUCOCORTICOIDS AND REGULATORY T CELLS ON CD28NULL T CELLS IN VITRO Jayesh Pandya, Mohammad Shahadat Hossain, Ingrid E. Lundberg, Vivianne Malmström. Rheumatology Unit, Department of Medicine, Karolinska University Hospital, Solna, Karolinska Institutet, Stockholm

3.12 INTRARENAL FOXP3+ REGULATORY T CELLS EXPANSION AND DECREASED NUMBER OF INFILTRATING CD4+ T CELLS IN MURINE LUPUS BY IL-2 THERAPY A. Rose1,2, JY Humrich1,2 , G. Riemekasten1,2

1German Rheumatism Research Center (DRFZ), a Leibniz Institute, Berlin, Germany, ; 2

University Hospital Charité, Rheumatology and Clinical Immunology, Berlin, Germany

3.13 INVESTIGATING T-CELL SUBSETS IN LYMPH NODE BIOPSIES OF AUTOANTIBODY POSITIVE INDIVIDUALS AND EARLY ARTHRITIS PATIENTS. T.H. Ramwadhdoebe1,2, J. Hähnlein, K.I. Maijer, J. Boorsma, L.J. van Boven3, D. M. Gerlag1, P. P. Tak1 , L.G.M van Baarsen1,2*

1Division of Clinical Immunology and Rheumatology, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands; 2Department of Experimental Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands; 3Department of Radiology, Kennermergasthuis, Haarlem, the Netherlands

3.14 PD-1 SIGNALLING AND LOW RORC MRNA CONTENT INFLUENCE THE TREG/TH17 BALANCE IN

HUMAN CORD BLOOD Sytze de Roock1, Sanne Hoeks1, Arie Jan Stoppelenburg1, Berent Prakken1, Ismé de Kleer2, Marianne Boes1

1Center for Molecular and Cellular Intervention CMCI, University Medical Center Utrecht, Utrecht, The Netherlands; 2Laboratory of Immunoregulation and Mucosal Immunology, Ghent University, Ghent, Belgium

3.15 RECONSTITUTED HIGH DENSITY LIPOPROTEIN (RHDL) MODULATES T EFFECTOR CELL FUNCTION IN A MURINE MODEL OF RHEUMATOID ARTHRITIS Tiniakou I 1,2, Zannis V.I 1,4, Boumpas D 3, Verginis P 3* and Kardassis D 1,2*

1University of Crete Medical School, Heraklion Greece; 2Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology of Hellas, Heraklion Greece; 3Biomedical Research Foundation of the Academy of Athens, Greece; 4Boston University Medical Center, Boston USA

3.16 SPECIFIC OVEREXPRESSION OF SYNOVIAL INTERLEUKIN-21+ CD4+ T CELLS CO-EXPRESSING TUMOR NECROSIS FACTOR IN RHEUMATOID ARTHRITIS: ROLE IN JOINT DESTRUCTION? M. Cristina Lebre1,2, Pedro L. Vieira3, Saida Aarrass1,2, Thomas Newsom-Davis3, Paul P. Tak1, and Gavin R. Screaton3

1Division of Clinical Immunology and Rheumatology, 2Department Experimental Immunology, Academic Medical Center/University of Amsterdam, Amsterdam, The Netherlands; 3Department of Immunology, Imperial College London, Hammersmith Campus, London, UK

3.17 SYNOVIAL FIBROBLASTS DIRECTLY INDUCE TH17 PATHOGENICITY VIA THE CYCLOOXYGENASE/PROSTAGLANDIN-E2 PATHWAY, INDEPENDENT OF IL-23 Sandra M.J. Paulissen(1,2), Jan Piet van Hamburg(1,2), Nadine Davelaar(1,2), Patrick S. Asmawidjaja(1,2), Johanna M.W. Hazes(1) and Erik Lubberts(1,2)

(1)Department of Rheumatology, Erasmus MC, University Medical Center Rotterdam, The Netherlands, (2) Department of Immunology, Erasmus MC, University Medical Center Rotterdam, The Netherlands.

3.18 SYNOVIAL FIBROBLASTS INHIBIT INFLAMMATORY T CELL RESPONSES THROUGH TRYPTOPHAN METABOLISM Lars-Oliver Tykocinski1, Anna-Marie Lauffer1, Antonia Bohnen1, Stefan Krienke1, Iris Oezen2, Ulrike Litzenburger2, Michael Platten2 and Hanns-Martin Lorenz1

1Department of Medicine V, Division of Rheumatology, University of Heidelberg, Heidelberg, Germany; 2Experimental Neuroimmunology, German Cancer Research Center (DKFZ), Heidelberg, Germany

3.19 THE MIR-148A IS INDUCED BY TWIST1 AND TBET AND PROMOTES THE SURVIVAL OF EFFECTOR MEMORY T HELPER 1 LYMPHOCYTES BY REGULATING THE PROAPOPTOTIC GENE BIM Claudia Haftmann1, Anna-Barbara Stittrich1, Kristyna Hradlikova1, Zhuo Fang1, Markus Bardua1, Kerstin Westendorf1, Gitta Anne Heinz1,2, Thomas Häupl3, Joachim Sieper3, Hyun-Dong Chang1, Nikolaus Rajewsky4, Hans-Martin Jäck5, Andreas Radbruch1* and Mir-Farzin Mashreghi1*

1 Deutsches Rheuma-Forschungszentrum (DRFZ) Berlin, Berlin, Germany; 2Institute of Molecular Immunology, Helmholtz Zentrum München, Munich, Germany ; 3 Charité Universitätsmedizin Berlin, Campus Charité Mitte, Berlin, Germany; 4 Max-Delbrueck-Center for Moleculare Medicine Berlin, Berlin, Germany ; 5 Division of Molecular Immunology, Friedrich-Alexander University Erlangen Nuernberg, Erlangen, Germany

3.20 TNF REGULATES CD3Ζ EXPRESSION OF T LYMPHOCYTES VIA SRC-LIKE ADAPTOR PROTEIN-DEPENDENT PROTEASOMAL DEGRADATION Barbara Érsek1, Viktor Molnár1, Andrea Balogh2, János Matkó2 , Andrew P. Cope3, Edit I. Buzás1, András Falus1, György Nagy1,4

1: Department of Genetics, Cell, and Immunobiology, Faculty of Medicine, Semmelweis University, 1089 Budapest, Hungary 2: Department of Immunology, Eötvös Loránd University, 1117 Budapest, Hungary 3: Division of Immunology, Infection and Inflammatory Disease, Academic Department of Rheumatology, Center for Molecular and Cellular Biology of Inflammation, King’s College School of Medicine, King’s College London, London WC2R 2LS, United Kingdom 4: Department of Rheumatology, Semmelweis University, Medical School, 1023 Budapest, Hungary

3.21 TNFΑ INFLUENCES RASGRP1 AND RASGRP3 EXPRESSION LEVELS IN PBMC, B AND T CELLS. Golinski ML1, Hiron M1, Guillou C1, Derambure C1, Boyer O3, Le Loët X2, Vittecoq O2, Lequerré T2. 1INSERM U905, Institute for Biomedical Research, University of Rouen, Rouen, France 2Department of Rheumatology, Rouen University Hospital & INSERM U905, Institute for Biomedical Research, University of Rouen, Rouen, France 3Department of Immunology, Rouen University Hospital & INSERM U905, Institute for Biomedical Research, University of Rouen, Rouen, France

3.22 UPREGULATED MICRORNA-182 EXPRESSION IS ASSOCIATED WITH ENHANCED CONVENTIONAL CD4+ T CELL PROLIFERATION IN SLE Tobias Alexander1,2, Claudia Haftmann2, Lars Templin2, Jens Humrich1,2, Gerd-Rüdiger Burmester1, Andreas Radbruch2, Falk Hiepe 1,2 and Mir-Farzin Mashreghi2 1 Medical Department, Division of Rheumatology and Clinical Immunology, Charité – University Medicine Berlin, Germany; 2 German Rheumatism Research Center (DRFZ), Berlin, Germany

3.23 VITAMIN D ANTAGONISES THE SUPPRESSIVE EFFECT OF INFLAMMATORY CYTOKINES UPON CTLA-4 EXPRESSION AND REGULATORY FUNCTION Louisa E. Jeffery1, Karim Raza1,2, David M. Sansom1 1MRC Centre for Immune Regulation, School of Immunity and Infection, Institute of Biomedical Research, University of Birmingham College of Medical and Dental Sciences, Birmingham, B15 2TT, UK., 2Department of Rheumatology, Sandwell and West Birmingham Hospitals NHS Trust, Birmingham, B18 7QH, UK.

3.24 ALTERATIONS IN GD T CELLS HOMEOSTASIS IN SYSTEMIC SCLEROSIS Tiago Carvalheiro1; Mariana Santiago2, Cláudia Silva1;3; Maria João Salvador2, José António P. Silva2, Artur Paiva1 1Centro do Sangue e da Transplantação de Coimbra | Instituto Português do Sangue e da Transplantação, I.P. Rheumatology Department. Centro Hospitalar e Universitario de Coimbra, Portugal 2Universidade de Aveiro

3.25 DELETION OF RBP-J IN A MURINE MODEL OF INFLAMMATORY ARTHRITIS REVEALS DIFFERENTIAL PRO-INFLAMMATORY CYTOKINE AND FOXP3 GENE EXPRESSION Soumya D. Chakravarty1, 2, Karmen Au2, Xiaoyu Hu2, and Lionel B. Ivashkiv1,2 1Division of Rheumatology, Hospital for Special Surgery and 2Department of Medicine, New York Presbyterian Hospital—Weill Cornell Medical Center & Weill Cornell Medical College of Cornell University, New York, NY

3.26 IDENTIFYING T-FOLLICULAR-HELPER-LIKE CELL INVOLVEMENT IN THE ORGANIZATION OF TUBULOINTERSTITIAL INFLAMMATION IN HUMAN LUPUS NEPHRITIS AND RENAL ALLOGRAFT REJECTION Liarski V*, Brandt D*, Kaverina N*, Henderson S*, Chang A#, Utset T*, Labno C§, Peng Y‡, Jiang Y‡, Giger M‡, Clark M* *Section of Rheumatology, Department of Medicine and Knapp Center for Lupus Research, University of Chicago, Chicago, IL 60637; #Department of Pathology, University of Chicago, Chicago, IL 60637; §Integrated Microscopy Core Facility and Knapp Center for Biomedical Discovery,University of Chicago, Chicago, IL 60637; ‡Department of Radiology, University of Chicago, Chicago, IL 60637 *Section of Rheumatology, Department of Medicine and Knapp Center for Lupus Research, University of Chicago, Chicago, IL 60637;

IV: Metabolism, Hormones and Autoimmunity 4.1 1,25(OH)2D3 MODULATES GENE EXPRESSION INVOLVED IN PHENOTYPE STABILITY AND

MIGRATION OF TH17 CELLS FROM PATIENTS WITH RHEUMATOID ARTHRITIS W. Dankers1,2, J.P. van Hamburg1,2, P.S. Asmawidjaja1,2, N. Davelaar1,2, S.M.J. Paulissen1,2, K. Wen1,2, A.M.C. Mus1,2, E.M. Colin1, J.P.T.M. van Leeuwen3, J.M.W. Hazes1 and E. Lubberts1,2. Departments of 1Rheumatology, 2Immunology and 3Internal Medicine, Erasmus MC, University Medical Center Rotterdam, The Netherlands.

4.2 ADIPOCYTES MODULATE T CELL FUNCTION THROUGH RELEASE OF LIPIDS Andreea Ioan-Facsinay1, Joanneke C.Kwekkeboom1, Sanne Westhoff1, Martin Giera2, Yoann Rombouts1, Tom W.J. Huizinga1, André Deelder2, Margreet Kloppenburg1, René E.M.Toes1 1 Dept. of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands 2 Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands

4.3 ADIPOCYTES MODULATE THE PHENOTYPE OF MACROPHAGES THROUGH SECRETED LIPIDS IR Klein-Wieringa*, SN Andersen*, JC Kwekkeboom*, M. Giera†, BJE de Lange-Brokaar*, GJVM van Osch‡, AM Zuurmond§, V Stojanovic-Susulic¶, RGHH Nelissen‖, TWJ Huizinga*, M Kloppenburg*, REM Toes*, A Ioan-Facsinay* * Dept. of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands; †Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands; ‡Dept. of Orthopaedics and Dept. of Otorhinolaryngology, Erasmus MC, University Medical Center, Rotterdam, the Netherlands; §TNO Leiden; The Netherlands; ¶Janssen Research & Development, LLC Spring House, Pennsylvania, USA; ‖Dept. of Orthopaedic surgery, Leiden University Medical Center, Leiden, The Netherlands

4.4 CIRCULATING METASTASIS PROMOTING PROTEIN S100A4 IN IDIOPATHIC INFLAMMATORY MYOPATHIES Pleštilová L1, Pecha O2, Mann H1, Cerezo LA1, Vencovský J1, Šenolt L1 1Institute of Rheumatology and Department of Rheumatology, 1st Faculty of Medicine, Charles University, Prague, 2Institute of Biophysics and Informatics, 1st Faculty of Medicine, Charles University, Prague

4.5 DO HIGH MOLECULAR WEIGHT ADIPONECTIN LEVELS ASSOCIATE WITH RADIOGRAPHIC PROGRESSION IN EARLY RHEUMATOID ARTHRITIS AND HAND OSTEOARTHRITIS? I I.R. Klein-Wieringa1, S.N. Andersen1, J.C. Kwekkeboom1, L. Herb-v.Toorn1, A.H.M. van der Helm-van Mil1, I. Meulenbelt2, T.W.J. Huizinga1, M. Kloppenburg1, R.E.M. Toes1, A. Ioan-Facsinay1

1 Dept. of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands; 2 Dept. of Molecular Epidemiology, Leiden, The Netherlands

4.6 EFFECTS OF CHOLIC ACID AND ITS DERIVATIVES IN EXPERIMENTAL ARTHRITIS. Silvia Hayer1, Emina Halilbasic2, Birgit Niederreiter1, Martin Willburger1, Peter Mandl1, Victoria Saferding1, Stephan Blüml1, Josef Smolen1, Michael Trauner2, Kurt Redlich1 1Medical University of Vienna, Department of Internal Medicine III, Division of Rheumatology; 2Medical University of Vienna, Department of Internal Medicine III, Division of Gasteroenterology

4.7 EVALUATION OF TISSUE INFLAMMATION AND ADIPOKINE EXPRESSION IN GASTRIC AND LUNG TISSUE OF SYSTEMIC SCLEROSIS (SSC) PATIENTS N. Lepper1, M. Peters1, M. Vasile1,2, E Roeb3, A. Günther4, U. Müller-Ladner1, E. Neumann1 1 Dept Internal Medicine and Rheumatology, Justus-Liebig-University Gießen, Kerckhoff Klinik, Bad Nauheim, Germany; 2 Dept of Clinic and Medical Therapy, Div Rheumatology, Sapienza, University of Rome, Italy; 3 Internal Medicine / Gastroenterology, University of Gießen, Gießen, Germany; 4 Internal Medicine / Pneumology, University of Gießen, Gießen, Germany;

4.8 GDF15, A MARKER OF LUNG INVOLVEMENT IN SYSTEMIC SCLEROSIS, IS INVOLVED IN ALTERED CYTOKINE SECRETION BY FIBROBLASTS, BUT DOES NOT IMPAIR FIBROSIS DEVELOPMENT Stijn Lambrecht1, Vanessa Smith1, Filip De Keyser1, Dirk Elewaut1

1, Department of Rheumatology, Ghent University

4.9 HOW OSTEOBLAST REGULATES ENERGY METABOLISM AND SYSTEMIC INFLAMMATION DEPENDENT OF FRA-2 EXPRESSION Aline Bozec1, Yubin Luo1, Christine Zech1, Maria Jimenez2,Latifa Bakiri2,Mirco Petrucelli2, Michael Amling3, Georg Schett1, Erwin Wagner2

1 Department of Medicine 3, Rheumatology and Immunology, University of Erlangen-Nuremberg, Nikolaus-Fiebiger-ZentrumGlueckstrasse 6, D-91054 Erlangen, Germany; 2 Genes, Development and Disease Group, F-BBVA-CNIO Cancer Cell Biology Program, Spanish National Cancer Research Centre (CNIO), E-28029 Madrid; 3 Department of Osteology and Biomechanics, University Medical Center, Hamburg-Eppendorf, Martinistrasse 52, D-20246 Hamburg, Germany.

4.10 HYDROGEN SULFIDE ATTENUATES STORE-OPERATED CA2+ ENTRY IN ENDOTHELIAL AND SMOOTH MUSCLE CELLS B. Kloesch1, S. Stranimaier2, G. Steiner1,3 and K. Schmidt2

1Ludwig Boltzmann Cluster Rheumatology, Balneology and Rehabilitation, Ludwig Boltzmann Institute of Rheumatology and Balneology, Kurbadstrasse 14, 1100 Vienna, Austria; 2Karl-Franzens-University Graz, Department of Pharmacology and Toxicology, Universitätsplatz 2, 8010 Graz, Austria; 3Medical University Vienna, Department of Internal Medicine III, Division of Rheumatology, Währinger Gürtel 18, 1090 Vienna, Austria

4.11 BASELINE ELEVATED SERUM LEVELS OF CALPROTECTIN AS INDEPENDENT MARKER FOR RADIOGRAPHIC SPINAL PROGRESSION IN ANKYLOSING SPONDYLITIS Maureen Turina1, Joachim Sieper2, Nataliya Yeremenko1,3, Hildrun Haibel2, Dominique Baeten1,3, Denis Poddubnyy2

1Department of Clinical Immunology and Rheumatology, Academic Medical Center/University of Amsterdam, The Netherlands. 2Department of Rheumatology, Charité, Campus Benjamin Franklin, Berlin, Germany.3Laboratory of Experimental Immunology, Academic Medical Center/University of Amsterdam, The Netherlands.

4.12 ENDOTHELIN-1 PROMOTES THE ENDOTHELIAL-TO-MESENCHYMAL TRANSITION IN CULTURES OF HUMAN ENDOTHELIAL CELLS. Stefano Soldano, Renata Brizzolara, Alberto Sulli, Marco Amedeo Cimmino, Bruno Seriolo, Paola Montagna, Maurizio Cutolo

Research Laboratory and Academic Unit of Clinical Rheumatology, Department of Internal Medicine, University of Genova, Genova

4.13 EVALUATION OF HISTOLOGY AND ADIPOKINE EXPRESSION OF SYNOVIAL TISSUE FROM PSORIATIC ARTHRITIS PATIENTS Elisabeth Schmitt, Elena Neumann, U Müller-Ladner Dept Internal Medicine and Rheumatology, Justus-Liebig-University Giessen, Kerckhoff-Klinik, Bad Nauheim, Germany

4.14 ROLE OF DIFFERENT ADIPONECTIN RECEPTORS IN ADIPONECTIN SIGNALING IN RHEUMATOID ARTHRITIS SYNOVIAL FIBROBLASTS. Khawaja K1, Frommer KW1, Schäffler A2, Büchler C2, Rehart S3, Lehr A3, Müller-Ladner U1, Neumann E1. 1 Department of Internal Medicine and Rheumatology, Justus-Liebig-University, Giessen, Kerckhoff-Klinik, Benekestrasse 2-8, D-61231 Bad Nauheim, Germany; 2 Department of Internal Medicine I, University of Regensburg, Regensburg, Germany; 3 Markus-Hospital, Frankfurt, Germany

4.15 SERUM LEVELS OF VISFATIN AND B-CELL ACTIVATING FACTOR OF THE TNF FAMILY CORRELATE WITH DISEASE ACTIVITY IN PATIENTS WITH MYOSITIS. Hulejová H1, Kryštůfková O1, Mann H1, Klein M1, Pavlíčková K2, Zámečník J2, Šenolt L1, Vencovský J1. 1Institute of Rheumatology, Experimental and Clinical Rheumatology, First Faculty of Medicine, Charles University in Prague, Czech Republic; 22nd Medical School and University Hospital Motol, Department of Pathology and Molecular Medicine, Charles University, Prague, Czech Republic

4.16 HIGH MOLECULAR WEIGHT ADIPONECTIN AND TUMOR NECROSIS FACTOR-Α INFLUENCE RHEUMATOID ADIPOSE-DERIVED MESENCHYMAL STEM CELLS FUNCTION. U. Skalska, W. Maslinski, E. Kontny Institute of Rheumatology, Department of Pathophysiology and Immunology, Warsaw

V: B cells and Autoimmunity 5.1 ABNORMAL CALCIUM INFLUX IN T AND B LYMPHOCYTES FROM SYSTEMIC LUPUS

ERYTHEMATOSUS PATIENTS IS RELATED TO STIM-1 OVER-EXPRESSION Yves Renaudineau1, Olivier Mignen2, Tinhinane Fali1, Miguel Burgos2, Divi Cornec1, Sandrine Jousse1, Alain Saraux1, Jacques-Olivier Pers1

1EA2216 Immunology, Pathology and Immunotherapy, Brest University and Brest Medical School Hospital, Brest, France; 2Inserm U613, Faculty of Medicine, Brest, France

5.2 ACCUMULATION OF CIRCULATING AUTOREACTIVE NAIVE B CELLS REVEAL DEFECTS OF EARLY B CELL TOLERANCE CHECKPOINTS IN PATIENTS WITH SJÖGREN´S SYNDROME Elisa Corsiero1, Nurhan Sutcliffe1, Hedda Wardemann2, Costantino Pitzalis1 and Michele Bombardieri1

1 Centre for Experimental Medicine & Rheumatology, William Harvey Research Institute, Barts and The London School of Medicine & Dentistry, Queen Mary University of London, John Vane Science Centre, Charterhouse Square, London EC1M 6BQ, UK; 2 Max Planck Institute for Infection Biology, 10117 Berlin, Germany

5.3 ALTERATIONS ON PERIPHERAL BLOOD B CELL COMPARTMENTS IN SYSTEMIC LUPUS ERYTHEMATOSUS: RELEVANCE FOR MONITORING LUPUS ACTIVITY AND THERAPY Isabel Silva1, Luis Inês2, Ana Henriques1, José António P. da Silva2,ArturPaiva1

1Cytometry Service of the Blood and Transplantation Center of Coimbra, Portugal; 2 Rheumatology Department. Centro Hospitalar e Universitário e Coimbra, Portugal

5.4 ANTI CARBAMYLATED PROTEIN ANTIBODIES (ANTI-CARP) ARE PRESENT IN ARTHRALGIA PATIENTS AND PREDICT THE DEVELOPMENT OF RHEUMATOID ARTHRITIS

Jing Shi,1* Lotte A. van de Stadt,2* E.W. Nivine Levarht,1 Tom W.J. Huizinga,1 René E.M. Toes,1 Leendert A. Trouw,1 Dirkjan van Schaardenburg3

1Leiden University Medical Center, Leiden; 2Jan van Breemen Research Institute | Reade, Sanquin Research, Amsterdam; 3Jan van Breemen Research Institute | Reade and VU University Medical Center, Amsterdam, The Netherlands; Mr. Shi and Dr. van de Stadt contributed equally to this work.

5.5 ANTIBODIES AGAINST DOMAIN I OF BETA2 GLYCOPROTEIN I IN ANTIPHOSPHOLIPID ANTIBODY SYNDROME Cecilia Chighizola1,2,3, Francesca Pregnolato1, Claudia Grossi1, Maria Gerosa2,3, Gary Norman4, Michael Mahler4, Maria Orietta Borghi1,2, Pier Luigi Meroni1,2,3

1 IRCCS Istituto Auxologico Italiano, Milan, Italy; 2 University of Milan, Italy; 3 Istituto G. Pini, Milan, Italy; 4 Inova Diagnostics, Inc., San Diego, CA, USA

5.6 ANTI-CARBAMYLATED PROTEIN ANTIBODIES ARE PRESENT IN MICE WITH COLLAGEN INDUCED ARTHRITIS. Jeroen Stoop, Bisheng Liu, Jing Shi, Diahann Jansen, Leendert Trouw, Rene Toes Leiden University Medical Center

5.7 AUTOANTIBODIES TO THE FIBRIN-DERIVED CITRULLINATED PEPTIDES Α36-50 AND Β60-74 ARE TWO DISTINCT NON-OVERLAPPING SUBFAMILIES OF ACPA THAT TOGETHER ALMOST SUMMARIZE THEIR REACTIVITY TO CITRULLINATED FIBRINOGEN AND TO CCP2 ANTIGENS. Cornillet M1, Sebbag M1, Verrouil E2,3, Magyar A4, Ruyssen-Witrand A2, Hudecz F4, Cantagrel A2, Serre G1,3 and Nogueira L1,3

1Laboratory of “Epidermis Differentiation and Rheumatoid Autoimmunity”, UMR CNRS 5165, INSERM U 1056, Toulouse III University; 2Rheumatology Center, University Hospital of Toulouse; 3Laboratory of Cell Biology and Cytology, University Hospital of Toulouse; Toulouse, France; 4Research Group of Peptide Chemistry, Department of Organic Chemistry, Hungarian Academy of Sciences, Eötvös Loránd University; Budapest, Hungary.

5.8 B CELL DEPLETION THERAPY IN A COHORT OF PATIENTS WITH SEROPOSITIVE ANDE SERONEGATIVE RHEUMATOID ARTHRITIS Velloso Feijoo ML, Martínez Pérez R, Mayordomo Gonzalez L, Marenco de la Fuente JL Rheumatology Unit. Valme University Hospital. Seville.

5.9 VALUES OF B LYMPHOCYTE SUBPOPULATIONS (HEALTHY POPULATION) USING FLOW CYTOMETRY. A STARTING POINT FOR ANY STUDY. Munoz A, Hernández R, Uceda J, León M, Gallo F, Paz J.A, Martínez R, Rodríguez S, Velloso ML, Cid N, González P, Mayordomo L, Rejón E, Marenco JL. Rheumatology Unit, Valme University Hospital, Seville, Spain

5.10 DELTA4BAFF ALTERNATIVE SPLICING IS REGULATED BY IFN-GAMMA AND SC35 PROTEIN Gabriel J. Tobόn1, Laurent Corcos2, Laëtitia Le Pottier1, Gwendal Dujardin2, Pierre Youinou1 and Jacques-Olivier Pers1

1EA2216 "Immunology & Pathology"; 2INSERM, UMR1078, Brest University and European University of Brittany, Brest, France

5.11 DETECTION OF ACPA PRODUCING B-CELLS BY A CITRULLINE PEPTIDE PANEL Eszter Szarka1, Krisztina Huber1, Judit Pozsgay1, Fruzsina Babos2, Tamás Gáti3, Anna Magyar2, Ferenc Hudecz 2, Bernadette Rojkovich3, György Nagy3, and Gabriella Sármay 1,4

1Department of Immunology, Eötvös Loránd University, Budapest; 2HAS-ELTE Research Group of Peptide Chemistry, Budapest; 3Buda Hospital of Hospitaller Brothers of St. John, Budapest; 4Immunology Research Group of the Hungarian Academy of Sciences at ELTE, Budapest, Hungary

5.12 DISAPPEARANCE AND REAPPEARANCE OF IGG, IGA AND IGM AUTOANTIBODY ISOTYPES AND IMMUNE COMPLEXES IN RITUXIMAB-TREATED SLE PATIENTS Barbro Persson1, Fionnuala McAree1, Malin Müller2, Tina Trollmo2, Ioannis Parodis2,

Thorunn Jonsdottir3 Vivianne Malmström2, Iva Gunnarsson2, Johan Rönnelid1

1Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden, and 2Unit of Rheumatology, Department of Medicine, Karolinska Institutet, Stockholm, Sweden, 3Department of Rheumatology, Landspitali University Hospital, Reykjavik, Iceland.

5.13 EFFECT OF RITUXIMAB ON B CELL SUBPOPULATIONS EXPRESSING THE 9G4 IDIOTYPE IN PATIENTS WITH RHEUMATOID ARTHRITIS Rita A. Moura1,2, Inmaculada de la Torre2,3, Maria J. Leandro2, Geraldine Cambridge2

1Rheumatology Research Unit, Instituto de Medicina Molecular, Lisbon, Portugal; 2Centre for Rheumatology Research, Division of Medicine, University College London, London, United Kingdom; 3Rheumatology Department, Gregorio Marañón Hospital, Madrid, Spain

5.14 HOMOCITRULLINE-REACTIVE ANTIBODIES CAN BE GENERATED FROM SYNOVIAL B-CELLS FROM ACPA-NEGATIVE RA PATIENTS Fiona Murray1, Lena Israelsson1, Johanna Steen1, Khaled Amara1, Jing Shi2, Lars Klareskog1, Leendert Trouw2 and Vivianne Malmström1

1. Rheumatology Unit, Department of Medicine at Karolinska University Hospital, Karolinska Institute, Solna, Stockholm, Sweden; 2. Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands.

5.15 HOW MICRORNAS CONTROL PLASMA CELLS Julia Schmid 1, Martina Porstner1, Andreas Brandl 1, Edith Roth 1, Thomas Winkler 2, Michael Bösl 3, Andreas Radbruch4, Jürgen Wittmann1,Hans-Martin Jäck 1

1 Division of Molecular Immunology, Nikolaus-Fiebiger-center, Department of Internal Medicine III, FAU Erlangen-Nürnberg, Erlangen, Germany, 2 Section of Hematopoesis, Department of Biology, FAU Erlangen-Nürnberg, Erlangen, Germany, 3 Max Planck Institute for Neurobiology, Martinsried, Germany, 4 Deutsches Rheumaforschungszentrum (DRFZ), Berlin

5.16 TNF-ALPHA RESPONSE OF SYNOVIAL FLUID MONOCYTE-MACROPHAGES TO ACPA IMMUNE COMPLEXES Cyril Clavel1,2, Florence Anquetil1,2, Laurent Zabraniecki3, Évelyne Verrouil1,3, Guy Serre1,2

and Mireille Sebbag1. 1Research Unit "Epidermis Differentiation and Rheumatoid Autoimmunity", UMR 5165 CNRS / 1056 INSERM / Université de Toulouse; 2Laboratory of Cell Biology and Cytology, CHU de Toulouse; 3Rheumatology Centre, CHU de Toulouse; Toulouse, France.

5.17 IGG4(+) B-CELL CLONES DOMINATE THE PERIPHERAL BLOOD IN IGG4-ASSOCIATED CHOLANGITIS Marieke Doorenspleet1#, Lucas Maillette de Buy Wenniger3#, Paul Klarenbeek1, Frank Baas2, Paul-Peter Tak1, Ulrich Beuers3*, Niek de Vries1*

1Department of Clinical Immunology and Rheumatology, 2Department of Genome Analysis, 3Department of Gastroenterology and Hepatology, all at Academic Medical Center / University of Amsterdam, The Netherlands #, * these authors contributed equally to this work

5.18 IMPACT OF RITUXIMAB ON SYNOVIAL GENE EXPRESSION Yasser M. El-Sherbiny, Sarah Churchman, Paul Emery, Edward M Vital and Frederique Ponchel. Division of Rheumatic and Musculoskeletal Disease, Leeds Institute of Molecular Medicine, St James’s University Hospital, Leeds, UK

5.19 IN AFRICAN PATIENTS WITH RHEUMATOID ARTHRITIS ACPA RECOGNIZE CITRULLINATED FIBRINOGEN AND THE IMMUNODOMINANT EPITOPES BORNE BY THE FIBRIN PEPTIDES Α36-50 AND Β60-74, LIKE IN CAUCASIANS. Nogueira L1,2 , Cornillet M1, Singwe-Ngandeu M3, Viatte S4, Bas S5,6, Gabay C5,7, Serre G1,2

1Laboratory of “Epidermis Differentiation and Rheumatoid Autoimmunity”, UMR CNRS 5165, INSERM U 1056, Toulouse III University, 2Laboratory of Cell Biology and Cytology, University Hospital of Toulouse; Toulouse, France. 3Unit of Rheumatology, School of Medicine, University of Yaoundé, Cameroun. 4Arthritis Research UK Epidemiology Unit, University of Manchester, 5Division of Rheumatology, University Hospitals of Geneva, 6Department of Genetics and Laboratory Medicine, 7Department of Pathology & Immunology; University of Geneva, School of Medicine, Genève, Suisse.

5.20 INTRAVENOUS IMMUNOGLOBULIN INDUCES FUNCTIONNAL SILENCING IN HUMAN B LYMPHOCYTES Sophie Hillion, Jean-François Seité, Carole Goutsmedt, Pierre Youinou and Jacques-Olivier Pers EA2216 \"Immunology & Pathology, Brest University and European University of Brittany

5.21 LACK OF IL-27R SIGNALING LEADS TO AN INTRINSIC B CELL DEFECT AND PROTECTION AGAINST CIA Odilia B.J. Corneth1,2, Patrick S. Asmawidjaja1,2, Laurens P. Kil3, Anne-Marie C. Mus1,2, Franka Luk1,2, Sandra M.J. Paulissen1,2, Rudi W. Hendriks3 and Erik Lubberts1,2

Departments of 1Rheumatology, 2Immunology and 3Pulmonary Medicine, Erasmus MC, University Medical Center, Rotterdam, The Netherlands.

5.22 MEMBRANE-BOUND AND SOLUBLE BAFF EXPRESSION BY HUMAN RHEUMATOID FIBROBLAST-LIKE SYNOVIOCYTES IN RESPONSE TO TLR STIMULATION N.W. Kam1, A. Filer2, C. Buckley2, M. Bombardieri1, C. Pitzalis1. 1William Harvey Research Institute, Queen Mary University of London, London, United Kingdom; 2Division of Immunity and Infection, College of Medical and Dental Sciences, University of Birmingham, UK

5.23 MULTIPARAMETER PHOSPHO-FLOW ANALYSIS OF B CELLS FROM PATIENTS WITH RHEUMATOID ARTHRITIS Daniel Szili1, Judit Pozsgay1, Zsuzsanna Banko1, Tamas Gati2, Gyorgy Nagy2,3, Bernadett Rojkovich2, Gabriella Sarmay1

1Department of Immunology, Eotvos Lorand University, Budapest, 2Buda Hospital of Hospitaller Brothers of St. John, Budapest, 3Department of Rheumatology, Semmelweis University, Budapest, Hungary.

5.24 NEUTROPHIL GRANULOCYTES RESPOND TO SURFACE-BOUND IMMUNE COMPLEXES CONTAINING ANTI-TYPE II COLLAGEN ANTIBODIES FROM RA PATIENTS Vivek Anand Manivel, Mohammed Mullazehi, Azita Sohrabian, Barbro Persson, Amir Elshafie, Efstathios Kavvadas, Johan Rönnelid Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala.

5.25 PROTEOMIC ANALYSIS OF ANTI-CCP IMMUNOGLOBULINS FOR THE IDENTIFICATION OF RHEUMATOID ARTHRITIS PATIENTS THAT REQUIRE EARLY AGGRESSIVE TREATMENT REGIMENS Martijn M. van Duijn1, Theo M. Luider1, Johanna M.W. Hazes2 and Radboud J.E.M.Dolhain2

Erasmus MC, University Medical Center, Rotterdam, The Netherlands: 1 Department of Neurology; 2 Department of Rheumatology

5.26 REGULATION OF EXPRESSION AND FUNCTION OF NEGATIVE IMMUNOMODULATORY RECEPTORS IN B-CELLS: IMPLICATIONS FOR THE PATHOGENESIS OF SYSTEMIC LUPUS ERYTHEMATOSUS Amalia Zampoylaki, Christianna Choulaki, Irini Gergianaki, Garyfalia Papadaki, Antonis Fanouriakis, Prodromos Sidiropoulos, Dimitrios T. Boumpas, George Bertsias 1Laboratory of Autoimmunity and Inflammation, Department of Rheumatology, Clinical Immunology and Allergy, University of Crete, Medical School, Heraklion, Greece; 2Institute

of Molecular Biology and Biotechnology, Foundation for Research and Technology, 71300, Heraklion, Greece

5.27 RO52 EXPRESSION IS A PROGNOSTIC FACTOR FOR SURVIVAL IN B CELL LYMPHOMA Susanna Brauner1, Wei Zhou1, Carin Backlin2, Tina M. Green3, 4, Ken He Young5, Björn Löfström6, Ingrid Lundberg1, Lars Møller Pedersen5, Michael Boe Møller4, 5, Christer Sundström7, Gunilla Enblad7, Eva Baecklund3 and Marie Wahren-Herlenius1

1 Rheumatology Research Unit, Department of Medicine, Karolinska Institutet, Stockholm, Sweden, 2 Unit of Rheumatology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden, 3 Institute of Clinical Research, University of Southern Denmark, 4 Odense University Hospital, Odense, Denmark, 5 MD Anderson Cancer Center, The University of Texas, Houston, USA, 6 Rheumatology Clinic, Malar hospital, Eskilstuna, Sweden, 7

Department of Genetics and Pathology, Rudbeck laboratory, Uppsala University, Uppsala, Sweden

5.28 SEMAPHORIN3A IS A POTENT B CELL REGULATORY MOLECULE IN SLE Zahava Vadasz and Elias Toubi Bnai-Zion Medical Center

5.29 SPONTANEOUS PRODUCTION OF ANTI-CITRULLINATED PROTEIN ANTIBODIES IN CULTURES OF PERIPHERAL BLOOD MONONUCLEAR CELLS AND SYNOVIAL FLUID MONONUCLEAR CELLS ISOLATED FROM PATIENTS WITH RHEUMATOID ARTHRITIS Priscilla F. Kerkman1, Yoann Rombouts, PhD1, Ellen I. H. van der Voort1, Leendert A. Trouw1, Tom W. J. Huizinga, MD PhD1, René E.M. Toes, PhD1, Hans U. Scherer, MD1

1Department of Rheumatology, Leiden University Medical Centre, Leiden, The Netherlands

5.30 SYSTEMIC INFLAMMATION AND B-CELLS IN RHEUMATOID ARTHRITIS M. Blits1, S. Vosslamber1, J. Lubbers1, S. de Ridder1, A.E. Oostlander1, G.J. Wolbink3, D. van Schaardenburg3, M.T. Nurmohamed2,3, D.M. Pegtel1, C.L. Verweij1,2 1Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands; 2Department of Rheumatology, VU University Medical Center, Amsterdam, The Netherlands; 3Department of Rheumatology, Jan van Breemen Research Institute | Reade, Amsterdam, The Netherlands;

5.31 THE ROLE OF BOB1 IN RHEUMATOID ARTHRITIS: POTENTIAL IMPLICATIONS FOR AUTOIMMUNITY Nataliya Yeremenko1, Tineke Cantaert1, Melissa van Tok1, Ioana Gofita1, Juan D. Canete2, Paul P.Tak1*, Hergen Spits3, Dominique Baeten1

1Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands, 2Hospital Clinic, Barcelona, Spain, 3Tytgat Institute for Liver and Intestinal Research, University of Amsterdam, Amsterdam, The Netherlands, *Currently also: GlaxoSmithKline, Stevenage, U.K.

VI: Microbes and Autoimmunity 6.1 ANTIBODY RESPONSE AGAINST PORPHYROMONAS GINGIVALIS AND MATRIX

METALLOPROTEINASE-3 ARE ASSOCIATED WITH ANTI-CITRULLINATED PROTEIN ANTIBODY IN RHEUMATOID ARTHRITIS, BUT ONLY MATRIX METALLOPROTEINASE-3 IS A PREDICTIVE FACTOR OF RESPONSE TO INFLIXIMAB. Rinaudo-Gaujous M1, Miossec P2, Blasco-Baque V3, Gaudin P4, Thomas T5, Moreau A1, Genin C1, Paul S1*, and Marotte H5*. (1) Laboratory of Immunology and immunomonitoring, CIC CIE3 Inserm Vaccinology, Hôpital Nord, CHU Saint-Etienne (France) (2) Clinical Immunology Unit, Departments of Immunology and rheumatology, Hôpital Edouard Herriot, CHU Lyon (France) (3) Institute of Cardiovascular and Metabolic Diseases, CHU Rangueil (France) (4) Grenoble Teaching

Hospital, CHU Hôpital Sud, Echirolles (France) (5) Department of Rheumatology, Hôpital Nord, CHU Saint-Etienne (France)

6.2 INDUCIBLE TERTIARY LYMPHOID STRUCTURES FORMATION IN A NOVEL MODEL OF SIALOADENITIS IN WILD-TYPE MICE D. Lucchesi1, M. Bombardieri1, F. Barone2, S. Nayar2, G. Proctor3, C.D. Buckley2, C. Pitzalis1. 1William Harvey Research Institute, Experimental Medicine and Rheumatology, London, United Kingdom.; 2University of Birmingham, Rheumatology Research Group, Birmingham, United Kingdom.; 3King's College, Oral Medicine and Pathology, London, United Kingdom.

6.3 IMMUNISATION WITH RECOMBINANT AUTOCITRULLINATED PORPHYROMONAS GINGIVALIS PEPTIDYLARGININE DEIMINASE INDUCES AUTOIMMUNITY TO ENOLASE AND ARTHRITIS IN DBA/1 MICE Anne-Marie Quirke, Dany Perocheau, Anna Montgomery and Patrick J. Venables Kennedy Institute of Rheumatology, Oxford University, London, United Kingdom.

6.4 PERIODONTOPATHOGENS IN RHEOMATOID ARTHTITIS AND PERIODONTITAL DISEASE O. Laugisch, B. Möller, T. Kantyka, P.J. Vernables, P.M. Villiger, A. Sculean, J. Potempa and S. Eick 1: Department of Periodontology, School of Dental Medicine, Freiburgstrasse 7, 3010 Bern, Switzerland; 2: Department of Rheumatology, Immunology and Allergology,, University of Bern, Inselspital, 3010 Bern, Switzerland ; 3: Institute of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University of Krakow, Gronostajowa 7, 30-387 Krakow, Poland; 4: Kennedy Institute of Rheumatology, Imperial College London, London, UK.; 5 Department of Oral Health and Rehabilitation, University of Louisville, 501 South Preston, Louisville KY 40202-1701, USA

6.5 SYNOVIAL LYMPHOID STRUCTURES SUPPORT EPSTEIN-BARR VIRUS PERSISTENCE AND AUTOREACTIVE PLASMA CELL INFECTION IN RHEUMATOID ARTHRITIS Cristina Croia1, Barbara Serafini2, Michele Bombardieri1, Stephen Kelly1, Frances Humby1 , Martina Severa3, Fabiana Rizzo3, Eliana Marina Coccia3, Paola Migliorini4, Francesca Aloisi2, Costantino Pitzalis1

1 Centre for Experimental Medicine and Rheumatology, William Harvey Research Institute, Queen Mary University of London, United Kingdom; 2 Department of Cell Biology and Neuroscience; and 3 Department of Infectious, Parasitic and Immune-mediated Diseases, Istituto Superiore di Sanità, Rome, Italy; 4 Department of Internal Medicine, University of Pisa, Italy

6.6 THE ROLE OF SYNDECAN-4 IN EXPERIMENTAL COLITIS Mareike Fröhling 1, Dominik Bettenworth2, Peter Paruzel1, Frank Echtermeyer3, Andreas Lügering2,4, Thomas Pap1, Athanasios Stratis 1

1Institut of Experimental Musculoskeletal Medicine, University Muenster, 48149 Muenster, Germany; 2Department of Medicine B, University Muenster, 48149 Muenster, Germany; 3Department of Anesthesiology and Intensive Care Medicine, Medical University Hannover, 30625 Hannover, Germany; 4MVZ Portal 10, Muenster, Germany

6.7 ALTERATIONS IN NAILFOLD VIDEOCAPILLAROSCOPY IN PATIENTS WITH GRANULOMATOSIS WITH POLYANGIITIS (WEGENER´S): AN OBSERVATIONAL STUDY Julia Uceda 1, Rosalía Martinez 1, María L Velloso 1, José L Marenco 1

1Rheumatology Department, Valme University Hospital, Sevilla, Spain

6.8 BIOLOGICAL THERAPIES IN JUVENILE IDIOPATHIC ARTHRITIS Velloso Feijoo ML, Martinez Perez R, Uceda Montańes J, Marenco de la Fuente JL. Rheumatology Unit. Valme University Hospital. Seville. Spain.

6.9 DIRECTED TRANSPORT OF MICRORNAS FROM APOPTOTIC CELLS TO PHAGOCYTES BY MEMBRANE-COATED VESICLES (MCVS) L Claßen, LO Tykocinski, F Wiedmann, S Krienke, A Lauffer, P Heyder, N Blank, M Schiller,

HM Lorenz Department of Internal Medicine V, Division of Rheumatology, University of Heidelberg, Heidelberg, Germany

6.10 HIGH DISEASE ACTIVITY AND EROSION RATE IN SUDANESE RHEUMATOID ARTHRITIS PATIENTS. Amir I Elshafie1, 2, Sahwa Nourein1, 3, Vivek Anand Manivel1, Azita Sohrabian1, Mawahib I. E. Elidrisi4, Elnour M. Elagib4, Musa A M Nur5, Johan Rönnelid1. 1 Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden.; 2 Department of Pathology and Microbiology, Alribat University Hospital, Khartoum, Sudan.; 3 Khartoum Fertility Center, Khartoum, Sudan.; 4 Rheumatology unit, Alribat University Hospital, Khartoum, Sudan.; 5 Rheumatology unit, Military Hospital, Omdurman, Sudan.

6.11 INTRA-ARTICULAR OVEREXPRESSION OF INTERLEUKIN-10 DIMINISHES CARTILAGE PROTEOGLYCAN DEPLETION IN STREPTOCOCCAL CELL WALL ARTHRITIS: A PROMISING CONCEPT FOR DISEASE-REGULATED GENE THERAPY Eline A. Vermeij, Mathijs G.A. Broeren, Miranda B. Bennink, Onno J. Arntz, Rheumatology Research & Advanced Therapeutics, Radboud University Nijmegen Medical Centre Presenting Author: Peter van Lent

6.12 LATERAL EPICONDYLE TENDON LESIONS TREATMENT WHIT PLATELET GROWTH FACTORS

Paz Gonzalez, Rosario Santos, Raquel Hernández Jose Luis Marenco de la Fuentevalme University Hospital, Seville Presenting Author: Maria Louisa Velloso

6.13 MINOR DISCREPANCY BETWEEN BMD OF SPINE AND HIP Poran Farahifard, Shahid Sadoughi, Mohammad Bagher Owlia, Shahid Sadoughi University of medical sciences, Yazd, Iran

6.14 MTOR DIRECTED MESENCHYMAL TISSUE RESPONSE TO INFLAMMATION IN ARTHRITIS T. Karonitsch1,2, K. Dalwigk2, M. Glehr3, B. Niederreiter2, C.W. Steiner2, J.S. Smolen2, H.P. Kiener2, G. Superti-Furga1

1 CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences ; 2 Division of Rheumatolotgy, Department of Medicine 3, Medical University of Vienna; 3 Department of Orthopaedic Surgery, Medical University of Graz

6.15 REFRACTORY CHRONIC ERYTHEMA NODOSUM AND TREATMENT WITH ANTI TNF J. Uceda, R. Hernández, L. Mayordomo, J.L. Marenco Rheumatology Department, Valme University Hospital, Seville, Spain Presenting Author: Maria Louisa Velloso

VII: Genetics and Epigenetics of Rheumatic Diseases 7.1 A GENETIC VARIANT IN THE REGION OF MMP-9 IS ASSOCIATED WITH SERUM LEVELS AND

PROGRESSION OF JOINT DAMAGE IN RHEUMATOID ARTHRITIS D.P.C. de Rooy1, A. Zhernakova1, R. Tsonaka2, A. Willemze1, B.A.S. Kurreeman1, R.E.M. Toes1, T.W.J. Huizinga1, J.J. Houwing-Duistermaat2, P.K. Gregersen3, A.H.M. van der Helm-van Mil1

1. Department of Rheumatology, Leiden University Medical Center, Leiden, the Netherlands; 2. Department of Medical Statistics, Leiden University Medical Center, Leiden, the Netherlands; 3. Feinstein Institute for Medical Research and North Shore–Long Island Jewish Health System, Manhasset, New York, USA

7.2 ALLOGRAFT INFLAMMATORY FACTOR 1 (AIF1) POLYMORPHISMS IN FRENCH CAUCASIANS WITH RHEUMATOID ARTHRITIS. Doua F. Azzouz1, Nathalie Balandraud1, 2, Sami B. Kanaan1, Isabelle Auger1, Marielle Martin1,

Fanny Arnoux1, Jean Roudier1,2 and Nathalie C. Lambert1. 1INSERM UMRs1097, Marseille; 2Rheumatology Department, Ste Marguerite Hospital, AP-HM, Marseille

7.3 ASSOCIATION OF CIRCULATING MIR-223 AND MIR-16 WITH DISEASE ACTIVITY IN PATIENTS WITH EARLY RHEUMATOID ARTHRITIS Mária Filková1, Caroline Ospelt1, Serena Vettori1, Ladislav Šenolt2, Heřman Mann2, Jiří Vencovský2, Karel Pavelka2, Beat A. Michel1, Renate E. Gay1, Steffen Gay1, Astrid Jüngel1

1Center of Experimental Rheumatology, University Hospital Zurich, Zurich, Switzerland, 2Institute of Rheumatology, Department of Clinical and Experimental Rheumatology of the 1st Faculty of Medicine, Charles University in Prague, Prague, Czech Republic

7.4 ASSOCIATION OF GALECTIN SINGLE NUCLEOTIDE POLYMORPHISMS WITH AUTOIMMUNE

DISEASES

Pál Z1,2, Antal P3, Srivastava SK1, Gál J4, André S5, Gordeeva E6, Nagy G1, Kaltner H5, Bovin

NV6, Molnár MJ2, Falus A1, Gabius HJ5, Buzás EI.1

1Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest,

Hungary;2 Department of Neurology, Semmelweis University, Budapest, 3University of

Technology and Economics, Budapest, Hungary,4 Department of Rheumatology, County

Hospital, Kecskemét, Hungary,5 Institute of Physiological Chemistry, Faculty of Veterinary

Medicine, Ludwig-Maximilians-University Munich, Munich, Germany, 6 Shemyakin and

Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow, Russia

7.5 COMBINED ANALYSIS OF EPIGENETIC AND TRANSCRIPTIONAL PROFILES IN DIFFERENT IMMUNE CELLS IDENTIFIES HOT SPOTS OF GENE REGULATION BY DNA METHYLATION Marc Bonin1, Stephan Flemming2, Stefan Günther2, Andreas Grützkau3, Thomas Häupl1

1 Department of Rheumatology and Clinical Immunology, Charité University Hospital, Berlin; 2 Institute of Pharmaceutical Sciences, University of Freiburg; 3 German Arthritis Research Center, Berlin

7.6 COMPARATIVE TRANSCRIPTOME ANALYSIS OF HUMAN AND MOUSE SYNOVIAL FIBROBLAST RESPONSES TO TNF Thomas Häupl1, Till Sörensen1, Bijana Smiljanovic1, Marc Bonin1, Andreas Grützkau2, Christoforos Nikolaou3, Ioannis Pandis3, George Kollias3, Anthony Rowe4

1Dept. of Rheumatology and Clinical Immunology, Charité University Medicine, Berlin; 2German Arthritis Research Center, Berlin; 3Biomedical Sciences Research Centre ‘Alexander Fleming’, Institute of Immunology, Vari; 4Janssen Research and Development

7.7 DIFFERENT GENETIC BACKGROUND OF DERMATOMYOSITIS AND POLYMYOSITIS IN A SINGLE CENTER COHORT. 1Remáková M, 1Škoda M, 1Svitálková T, 1Faustová M, 1Pleštilová L, 2Betteridge Z, 1Mann H, 1Vencovský J, 1Kryštůková O, 1Novota P 1Institute of Rheumatology, Prague, Czech Republic; 2Royal National Hospital for Rheumatic Diseases, Bath, United Kingdom

7.8 DNA DEMETHYLATION IN SALIVARY GLAND EPITHELIAL CELLS FROM PATIENTS WITH PRIMARY SJÖGREN’S SYNDROME MAY BE ASCRIBED TO INFILTRATING B CELLS Yves Renaudineau1,2, Yosra Thabet1,3, Christelle Le Dantec1, Ibtissem Ghedira3, Valérie Devauchelle1,4, Divi Cornec1,4, Jacques-Olivier Pers1 1Research Unit EA2216 "Immunology, Pathology and Immunotherapy", European University of Brittany, Brest University Medical School Hospital, Brest, France; 2Laboratory of Immunology and Immunotherapy, CHRU Morvan, Brest, France; 3Research Unit 03UR/07-02 “Autoimmunity and Allergy”, Faculty of Pharmacy, Monastir, Tunisia; 4Unit of Rheumatology, CHRU Cavale Blanche, Brest, France

7.9 DOES TELOMERE SHORTENING IN WOMEN WITH RHEUMATOID ARTHRITIS PREDICT X CHROMOSOME INACTIVATION BIAS? Sami Barna Kanaan,1 Onur Emre Onat,2 Nathalie Balandraud,1,3 Doua F Azzouz,1 Jean Roudier,1,3 Tayfun Ozcelik2 and Nathalie C Lambert1 1INSERM UMR1097, Marseille, France; 2Department of Molecular Biology and Genetics, Bilkent University, Ankara, Turkey; 3Rheumatology department, Hôpital Sainte Marguerite, Marseille, France

7.10 GENETIC VARIANTS IN THE IL-4 AND IL-4 RECEPTOR GENES IN ASSOCIATION WITH THE SEVERITY OF JOINT DAMAGE IN RHEUMATOID ARTHRITIS: A STUDY IN SEVEN COHORTS A. Krabben1, A. G. Wilson2, D. P. C. de Rooy1, A. Zhernakova1,3, E. Brouwer4, E. Lindqvist5, T. Saxne5, G. Stoeken1, J. A. B. van Nies1, R. Knevel1, T. W. J. Huizinga1, B. Koeleman6, R. Toes1, P. K. Gregersen7, A. H. M. van der Helm-van Mil1

1Department of Rheumatology, Leiden University Medical Center, The Netherlands; 2Department of Musculoskeletal Sciences, University of Sheffield, Sheffield, United Kingdom; 3Genetics Department, University of Groningen, University Medical Center, Groningen, The Netherlands; 4Department of Rheumatology and Clinical Immunology, University of Groningen, University Medical Center, Groningen, The Netherlands; 5Department of Rheumatology, Lund University, Skåne university Hospital, Lund, Sweden; 6Department of Medical Genetics, Complex Genetics Section, Utrecht, The Netherlands ; 7Feinstein Institute for Medical Research and North Shore–Long Island Jewish Health System, Manhasset, New York, USA

7.11 GENETIC VARIATION IN PROMOTER SEQUENCE OF B-CELL-ACTIVATING FACTOR OF THE TNF FAMILY (BAFF) IN PATIENTS WITH IDIOPATHIC INFLAMMATORY MYOPATHIES (IIM) Faustová M, Pleštilová L, Hulejová H, 1Pecha O, 2Betteridge Z, Mann H, Půtová I, Vencovský J, Novota P, Kryštůfková O Institute of Rheumatology and Department of Rheumatology, 1st Faculty of Medicine, Charles University, Prague, 1 Institute of Biophysics and Informatics, 1st Faculty of Medicine, Charles University Prague, 2 Rheumatology Department, Royal National Hospital for Rheumatic Diseases, Bath, United Kingdom

7.12 IDENTIFICATION OF THE SEMAPHORIN RECEPTOR PLEXIN-A2 AS A CANDIDATE GENE FOR SUSCEPTIBILITY TO ANKYLOSING SPONDYLITIS Chatzikyriakidou A, Voulgari PV, Drosos AA Rheumatology Clinic, Department of Internal Medicine, Medical School, University of Ioannina

7.13 IRAK1 RS3027898 POLYMORPHISM: A VARIANT IMPLICATED IN THE PATHOGENESIS OF MORE THAN ONE INFLAMMATORY DISEASE Chatzikyriakidou A, Voulgari PV, Drosos AA Rheumatology Clinic, Department of Internal Medicine, Medical School, University of Ioannina

7.14 IRF5 POLYMORPHISMS IN SYSTEMIC SCLEROSIS

Stock, C.1, Sato, H. 1, Fonseca, C. 2, Wells, A.U. 1, Denton C.P.2, Abraham D.J.2, Lindahl, G.1,

Renzoni, E.A. 1

1Interstitial Lung Disease Unit, Royal Brompton Hospital, London, United Kingdom; 2 Center

for Rheumatology, Royal Free Hospital, London, United Kingdom

7.15 LACK OF ASSOCIATION OF VARIANTS PREVIOUSLY ASSOCIATED WITH ANTI-TNF MEDICATION RESPONSE IN RHEUMATOID ARTHRITIS PATIENTS: RESULTS FROM A HOMOGENEOUS GREEK POPULATION M.I. Zervou1, P. Sidiropoulos2, E. Myrthianou1, I. Flouri2, D. Plant3, P. Rapsomaniki2, A. Barton3, D.T. Boumpas5,6, and G.N. Goulielmos1 1Laboratory of Molecular Medicine and Human Genetics, Medical School of Crete, Greece;

2Department of Rheumatology, Clinical Immunology and Allergy, University Hospital of Heraklion, Greece; 5Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, Heraklion, Greece; 6Medical School, University of Athens , Greece

7.16 LACK OF REPLICATION OF PTPRC GENE AS A PREDICTOR OF RESPONSE TO ANTI-TUMOR NECROSIS FACTOR THERAPY IN PATIENTS WITH RHEUMATOID ARTHRITIS Helena Canhăo1, Ana Rodrigues1, Maria Jose Santos1, Diana Carmona-Fernandes1, José Costa2, Helena Santos3, Jaime Branco4, Robert Plenge5, Daniel Solomon5, Jacome Armas6, José António Silva7, Joăo Eurico Fonseca1, Elizabeth Karlson5 1Rheumatology Research Unit, Instituto Medicina Molecular, Lisbon, Portugal 2Rheumatology Department, Unidade Saúde Alto Minho, PonteLima, Portugal 3Instituto Portuguęs Reumatologia, Lisbon, Portugal 4CEDOC, Lisbon, Portugal 5Division of Rheumatology, Brigham and Women's Hospital, Boston, USA 6SEEBMO, Azores, Portugal 7Rheumatology Department, Centro Hospitalar Universidade Coimbra, Coimbra, Portugal

7.17 MICROARRAY GENE EXPRESSION PROFILING OF RHEUMATOID ARTHRITIS PATIENTS FOR PREDICTION OF RESPONSE TO METHOTREXATE TREATMENT Mans, K.; Tandon, N.; Sohnrey, C.; Bolle, S.; Grützkau, A.; Burmester, G.R.; Häupl, T.; Stuhlmüller, B. Department of Rheumatology and Clinical Immunology, Charité University Medicine Berlin, Charitéplatz 1, 10117 Berlin

7.18 PATHWAYS INDUCED BY ETANERCEPT AND ADALIMUMAB ARE SPECIFIC IN RA PATIENTS Romain Normanda*, Celine Deramburea*, Martine Hirona, Xavier Le Loëtab, Olivier Vittecoqab, Thierry Lequerréab a INSERM 905, Faculty of Medicine and Pharmacy, IRIB, Rouen, France. b Rheumatology Department, Rouen University Hospital, France.* Equal contributors

7.19 POSITIVE ASSOCIATION OF TAGAP RS212389 POLYMORPHISM WITH RHEUMATOID ARTHRITIS SUSCEPTIBILITY Chatzikyriakidou A, Voulgari PV, Drosos AA Rheumatology Clinic, Department of Internal Medicine, Medical School, University of Ioannina

7.20 RESPONSE TO INFLIXIMAB THERAPY CAN BE PREDICTED USING DISTINCT, NON-OVERLAPPING GENE PANELS OF PERIPHERAL BLOOD GENE EXPRESSION IN RHEUMATOID ARTHRITIS AND CROHNÍS DISEASE Zoltán Szekanecz1, Bertalan Meskó2, Szilárd Poliska2, 3, Andrea Váncsa1, Károly Palatka4, Zsolt Holló5, Gábor Zahuczky6, János Podani7, Attila Horváth6, László Steiner6, 1Department of Rheumatology, Institute of Medicine; 2Department of Biochemistry and Molecular Biology; 3Clinical Genomics Center; 4Department of Gastroenterology, Institute of Medicine; University of Debrecen, Medical and Health Science Center, Debrecen, Hungary; 5EGIS Pharmaceuticals Plc., Budapest, Hungary; 6UD-GenoMed, Ltd., Debrecen, Hungary; 7Institute of Biology, Loránd Eötvös University, Budapest, Hungary

7.21 SUPPRESSION OF HDAC5 EXPRESSION BY INFLAMMATORY CYTOKINES IS REQUIRED TO PROMOTE CXCL CHEMOKINE PRODUCTION IN RA FLS C. Angiolilli1, A.M. Grabiec1, L.G.M van Baarsen1, P.P. Tak1.2, D.L. Baeten1, K. A. Reedquist1

1Department of Experimental Immunology and Department of Clinical Immunology and Rheumatology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands. ; 2Currently also: GlaxoSmithKline, Stevenage, U.K.

7.22 THE ETS1 TRANSCRIPTION FACTOR MAY PREDISPOSE TO RHEUMATOID ARTHRITIS SUSCEPTIBILITY Chatzikyriakidou A, Voulgari PV, Drosos AA

Rheumatology Clinic, Department of Internal Medicine, Medical School, University of Ioannina

7.23 THE HLA LOCUS CONTAINS NOVEL FETAL SUSCEPTIBILITY ALLELES FOR CONGENITAL HEART

BLOCK WITH SIGNIFICANT PATERNAL INFLUENCE

Sabrina Görgen1*, Therese Östberg1*, Stina Salomonsson1, Bo Ding2, Håkan Eliasson3, Anders

Mälarstig1, Lars Alfredsson2, Lars Klareskog1, Anders Hamsten1, Tomas Olsson4, Tomas

Axelsson5, The Swedish Congenital Heart Block Study Group6, Fredrik Gadler1, Anders

Jonzon7, Sven-Erik Sonesson3, Ingrid Kockum4, Marie Wahren-Herlenius1

1Department of Medicine, 2Department of Environmental Medicine, 3Department of

Women’s and Children’s Health, 4Department of Neuroscience, Karolinska Institutet,

Stockholm, 5Department of Medical Sciences, Uppsala University, 6www.combinesweden.se, 7Department of Women’s and Children’s Health, Uppsala

University, Uppsala, Sweden

*These authors contributed equally

7.24 THE PENTANUCLEOTIDE INSERTION IN HSPA1B GENE IS ASSOCIATED WITH IDIOPATHIC INFLAMMATORY MYOPATHY. Svitalkova T, Remakova M, Faustova M, Plestilova L, Mann H, Vencovsky J, Novota P Institute of Rheumatology, Prague, Czech Republic

7.25 GENE-GENE INTERACTIONS IN INTERFERON PATHWAY GENE-MUTATIONS IN EUROPEAN AND AMERICAN SCLERODERMA COHORTS Pravitt R. Gourh1, Filemon K. Tan2, Blanca Rueda3, Frank C. Arnett2, Shervin Assassi2, John D. Reveille2, Timothy RD Radstake4, Sandeep K. Agarwal2, Javier Martin5 and Maureen D. Mayes2

1National Institutes of Health, Bethesda, MD, 2University of Texas Health Science Center at Houston, Houston, TX, 3Facultad de Ciencias de la Salud. Universidad de Granada, Granada, Spain, 4 University Medical Centre Utrecht, Utrecht, The Netherlands, 5Instituto de Parasitologia y Biomedicina Lopez-Neyra (CSIC), Granada, Spain

VIII: Bone/Cartilage biology 8.1 ALARMINS S100A8/A9 CAUSE OSTEOPHYTE FORMATION IN EXPERIMENTAL

OSTEOARTHRITIS WITH HIGH SYNOVIAL INVOLVEMENT Rik F. P. Schelbergen1, A. B. Blom1, W de Munter1, A. Sloetjes1, T. Vogl2, J. Roth2, W. B. van den Berg1, P.L.E.M. van Lent1

1 Dept. of Rheumatology, Radboud Univ. Med. Ctr., Nijmegen, NETHERLANDS; 2 Inst. of Immunology, Univ. of Muenster, Muenster, GERMANY

8.2 ANTI CITRULLINATED PROTEIN ANTIBODIES FROM SYNOVIAL FLUID OF RHEUMATOID ARTHRITIS PATIENTS ENHANCE OSTEOCLASTOGENESIS Akilan Krishnamurthy1, Nancy Vivar Pomiano1, Catia Cerqueira1, Elena Ossipova1, Karin Lundberg1, Ulrike Harre2, Vivianne Malmström1, Per Johan Jakobsson1, Lars Klareskog3, Georg Schett2 and Anca Irinel Catrina1

1Rheumatology unit, Karolinska University Hospital, Karolinska Institute, Stockholm, Sweden, 2Institute for Clinical Immunology, University of Erlangen-Nuremberg, Erlangen, Germany, 3Karolinska Institute, Stockholm, Sweden

8.3 DEFICIT OF S100A4 PREVENTS JOINT DESTRUCTION AND SYSTEMIC BONE LOSS IN HTNFTG MOUSE MODEL Michal Tomcik (1, 2), Christina Böhm (2), Carina Scholtysek (2), Lucie Andres Cerezo (1), Wolfgang Baum (2), Clara Dees (2), Christian Beyer (2), Jerome Avouac (2, 3), Pawel Zerr (2), Katrin Palumbo (2), Alfiya Distler (2), Radim Becvar (1), Oliver Distle

(1)Institute of Rheumatology and Department of Rheumatology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic, (2)Department of Internal Medicine III and Institute for Clinical Immunology, University of Erlangen-Nuremberg, Erlangen, Germany (3)Paris Descartes University, Rheumatology A department, Cochin Hospital, Paris, France, (4)Center of Experimental Rheumatology and Zurich Center of Integrative Human Physiology, University Hospital Zurich, Switzerland, (5) Institute of Cancer Biology, Danish Cancer Society, Copenhagen, Denmark

8.4 FC-GLYCOSYLATION DETERMINES OSTEOCLASTOGENIC ACTIVITY OF IMMUNE COMPLEXES U. Harre1, R. Pfeifle1, A. Lux2, S. Frühbeißer3, F. Gröhn3, G. Krönke1, F. Nimmerjahn3, M. Herrmann1, G. Schett1

1 Department of Internal Medicine 3, Friedrich-Alexander University of Erlangen-Nuremberg, Germany; 2 Department of Biology, Friedrich-Alexander University of Erlangen-Nuremberg, Germany; 3 Department of Chemistry and Pharmacy and Interdisciplinary Center for Molecular Materials, Friedrich-Alexander University of Erlangen-Nuremberg, Germany

8.5 FIBROBLAST ACTIVATION PROTEIN ALPHA IN INFLAMMATORY BONE DESTRUCTION Christina Wunrau, Corinna Wehmeyer, Marianne Heitzmann, Thomas Pap, Berno Dankbar Institute of Experimental Musculoskeletal Medicine-IEMM, University Hospital Muenster, Muenster, Germany.

8.6 FRZB IS A CRITICAL MODULATOR OF CANONICAL WNT SIGNALLING IN CARTILAGE BIOLOGY Thysen S, Cailotto F, Luyten FP, Lories R Laboratory of Tissue Homeostasis and Disease, Skeletal Biology and Engineering Research Center, Department of Development and Regeneration, KU Leuven, Belgium

8.7 FUNCTIONAL IMPAIRMENT IN AN ANIMAL MODEL FOR RHEUMATOID ARTHRITIS ASSESSED AS CHANGES IN GAIT IS DUE TO JOINT DESTRUCTION BUT NOT SYNOVIAL INFLAMMATION PER SE Gregor Bauer, Martin Willburger, Constantin Aschauer, Aurica Jelinek, Tetyana Shvets, Birgit Niederreiter, Josef Smolen, Kurt Redlich, Silvia Hayer Medical University of Vienna, Department of Internal Medicine III, Division of Rheumatology, 1090 Vienna, Austria

8.8 HYDROGEN SULFIDE INHIBITS IL-1Β STIMULATION OF FIBROBLAST-LIKE SYNOVIOCYTES FROM OSTEOARTHRITIS PATIENTS IN A 3-D MODEL D. Sieghart1, HP. Kiener2, G. Steiner1,2, B. Kloesch1 1Ludwig Boltzmann Cluster Rheumatology, Balneology and Rehabilitation, Ludwig Boltzmann Institute of Rheumatology and Balneology, Vienna, Austria; 2Medical University of Vienna, Department of Internal Medicine III, Division of Rheumatology, Vienna, Austria

8.9 CHARACTERIZATION OF CELL METABOLIC CHANGES IN OSTEOARTHRITIC LABRUM CELLS Juchtmans Nele1, Dhollander Aad A.M., Lambrecht Stijn1, Elewaut Dirk1

1Department of Rheumatology, Ghent University, Ghent, Belgium

8.10 MAPK-ACTIVATED PROTEIN KINASE-3 (MK3) REGULATES OSTEOCLAST DIFFERENTIATION AND BONE HOMEOSTASIS Tobias Braun1, Gisela Ruiz Heiland1, Georg Schett1, Jochen Zwerina1,2

1Department of Medicine 3, University of Erlangen-Nuremberg, Erlangen, Germany. 2Ludwig Bolzmann Institut of Osteology at the 4th medical Department, Hanusch Hostpital, Vienna, Austria.

8.11 RANKL EXPRESSION IS LOWER ON T AND B LYMPHOCYTES AND RANKL+ CELLS TEND TO ACCUMULATE IN CIRCULATION OF RHEUMATOID ARTHRITIS PATIENTS TREATED WITH TNF BLOCKERS Perpétuo IP1, Marques R2, Ponte C2, Canhão H1,2, Fonseca JE1,2

1 Rheumatology Research Unit, Instituto de Medicina Molecular da Faculdade de Medicina da Universidade de Lisboa, Portugal; 2 Lisbon Academic Medical Centre, Portugal

8.12 SMALL UBIQUITIN RELATED MODIFIER-1 (SUMO-1) REGULATES OSTEOCLASTOGENESIS Svetlana Frank1, Daniel Umlauf 1, Anja Hillmann1, Olli A. Jänne 2, Thomas Pap1

1Institute of Experimental Musculoskeletal Medicine, University of Muenster, Muenster Germany; 2Univ Helsinki, Inst Biomed Physiol, Biomedicum, Helsinki, Finland

8.13 SYNDECAN-4 FUNCTION IS ESSENTIAL FOR MATRIX REMODELING UNDER INFLAMMATORY CONDITIONS, BUT DISPENSABLE DURING EMBRYOGENESIS Jessica Bertrand1, Richard Stange2, Heriburg Hidding1, Frank Echtermeyer3, Giovanna Nalesso4, Lars Godmann1, Melanie Timmen1, Peter Bruckner5, Francesco Dell'Accio4, Michael J. Raschke2, Thomas Pap1, and Rita Dreier5

1 Institute for Experimental Musculoskeletal Medicine, University Hospital Muenster, Germany; 2Dept. of Trauma, Hand and Reconstructive Surgery; University Hospital Muenster, Germany; 3Dept. of Anaesthesiology and Intensive Care Medicine, Medical School Hanover, Germany; 4 Centre for Experimental Medicine and Rheumatology Queen Mary London, UK; 5Institute for Physiological Chemistry and Pathobiochemistry, University Hospital Muenster, Germany

8.14 THE ANTI-PROLIFERATIVE FUNCTION OF RSK2 IN SYNOVIAL FIBROBLASTS PROTECTS AGAINST TNF-ALPHA-INDUCED JOINT DESTRUCTION IN INFLAMMATORY ARTHRITIS Anja Derer1*, Christina Böhm1*, Bettina Herbort1, Sybille Böhm2, Kirsten Neubert3, Michael Stock1, Christine Zech1, Georg Schett1, Axel J. Hueber1* and Jean-Pierre David1,4*. 1Department of Internal Medicine 3, Rheumatology and Immunology, University Hospital, Erlangen, Germany, 2Department of Biology, University of Erlangen-Nuremberg, 3 Department of Dermatology, Research Modul II, University Hospital, Erlangen, 4 Institute for Osteology and Biomechanics, University Medical Center Hamburg-Eppendorf, Hamburg, Germany. *contributed equally

8.15 THE FOCAL CONTACT PROTEIN LASP-1 MODULATES THE MIGRATION CAPACITY OF SYNOVIAL FIBROBLASTS Adelheid Korb-Pap1, Denise Beckmann1, Jan Hillen1, Marianne Heitzmann1,2, Catherine S. Chew3, Stefan Butz4, Dietmar Vestweber4, Hermann Pavenstädt2 and Thomas Pap1

1Institute of Experimental Musculoskeletal Medicine, University Hospital Muenster, Germany, 2Internal Medicine D, Department of Nephrology and Rheumatology, University Hospital Muenster, Germany, 3Institute of Molecular Medicine and Genetics, Medical College of GA, USA, 4Max-Planck-Institute for Molecular Biomedicine, University of Muenster, Germany

8.16 THE ROLE OF ADIPOCYTOKINES IN OSTEOARTHRITIS OSTEOPHYTE FORMATION S Junker1, G Krumbholz1, KW Frommer1, S Rehart2, U Lange1, J Steinmeyer3, M Rickert4, G Schett5, U Müller-Ladner1, E Neumann1

1 Dept Internal Medicine and Rheumatology, Justus-Liebig-University Giessen, Kerckhoff-Klinik Bad Nauheim, Germany; 2 Dept Orthopedics and Trauma Surgery, Markus-Hospital, Frankfurt, Germany; 3 Dept Experimental Orthopedics, University Hospital Giessen and Marburg, Giessen, Germany; 4 Dept Orthopedics and Orthopedic Surgery, University Hospital Giessen and Marburg, Giessen, Germany; 5 Department of Internal Medicine 3, Rheumatology and Immunology, Friedrich-Alexander-University of Erlangen-Nürnberg, Erlangen, Germany

8.17 THE ROLE OF CXCR2 SIGNALLING IN ARTICULAR CARTILAGE HOMEOSTASIS Sherwood, J.1,2, Bertrand, J.1, Nalesso, G.2, Achan, P.2, Pitzalis, C.2, Pap, T.1, Dell’Accio, F.2

1Institute for Experimental Musculoskeletal Medicine, University Hospital Münster, Münster, Germany; 2Centre for Experimental Medicine and Rheumatology, William Harvey Research Institute, Queen Mary University of London, U.K.

8.18 VISFATIN/ PBEF IN BONE REMODELLING OF RHEUMATOID ARTHRITIS G Krumbholz1, S Junker1, A Lehr2, M Rickert3, G Schett4, U Lange1, S Rehart2, U Müller-Ladner1, E Neumann1

1 Dept Internal Medicine and Rheumatology, Justus-Liebig-University Gießen, Kerckhoff-Klinik Bad Nauheim, Germany; 2 Dept Orthopedics and Trauma Surgery, Markus-Hospital, Frankfurt, Germany; 3 Dept Orthopedics and Orthopedic Surgery, University Hospital Gießen and Marburg, Gießen, Germany; 4 Medical Clinic 3: Rheumatology and Immunology, Erlangen, Germany

IX: Novel Therapeutic Targets 9.1 AVB3 INTEGRIN INHIBITION WITH CILENGITIDE BOTH PREVENTS AND TREATS COLLAGEN

INDUCED ARTHRITIS Despoina Sykoutri1, Nisha Geetha2, Silvia Hayer1, Peter Mandl1, Josef S. Smolen1, Gerald Prager2, Kurt Redlich1

1Department of Rheumatology, 2Department of Oncology, Medical University of Vienna, Austria

9.2 THE PHOSPHOINOSITIDE 3-KINASE PATHWAY REGULATES FIBROBLAST-LIKE SYNOVIOCYTES INVASION Beatrix Bartok and Gary S. Firestein UC San Diego School of Medicine, La Jolla, CA

9.3 DELETION OF MPGES-1 AFFECTS FATTY ACID COMPOSITION AND EICOSANOID PROFILES IN MICE Helena Idborg1, 2, Petter Olsson3, Patrick Leclerc1, Joan Raouf1, Per-Johan Jakobsson1,Marina Korotkova1, 2

1 Rheumatology Unit, Department of Medicine, Karolinska Institutet, Sweden; 2 Actar AB, Solna, Sweden; 3 Department of Analytical Chemistry, Stockholm University, Sweden

9.4 FLT3L-DEPENDENT CD103+ DC ARE CRUCIAL FOR THE INITIATION AND MAINTENANCE OF COLLAGEN-INDUCED ARTHRITIS M. Inês Ramos1,2, Saida Aarrass1,2, S.E Jacobsen3, Paul P. Tak1 and M. Cristina Lebre1,2

1Departmtment of Clinical Immunology and Rheumatology, 2Department of Experimental ImmunologyAcademic Medical Center/University of Amsterdam, Amsterdam, The Netherlands; 3 Stem Cell Biology Laboratory, University of Oxford, UK

9.5 IDENTIFICATION AND VALIDATION OF A PROTEIN COMBINATION INCLUDING S100A9 ABLE TO PREDICT THE RESPONSE TO THE MTX/ETANERCEPT ASSOCIATION IN RHEUMATOID ARTHRITIS PATIENTS A. Obry 1,2, T. Lequerré 1,, J. Hardouin 2, O. Boyer 1,3, 4,O. Vittecoq 1,4 ,P. Cosette 2

1Inserm905, Institute for Biomedical Research, University of Rouen, Rouen, 2UMR 6270 CNRS, Plate-Forme d'Analyse Protéomique PISSARO, Faculté des Sciences , 3Department of Immunology, Rouen University Hospital, 4Department of rheumatology, Rouen University Hospital

9.6 IDENTIFICATION OF NEW POTENTIAL THERAPEUTIC TARGETS FOR THE TREATMENT OF RHEUMATOID ARTHRITIS: ENTPD1 (CD39) AND 5NTE1 (CD73) Jonathan D Finn1,2, Lisa van Baarsen2, Jan van Ittersum1,2, Ciska Braam1,2. Maria C Lebre2, Paul P Tak1,2, Margriet J Vervoordeldonk1,2

1 Arthrogen B.V., Amsterdam, The Netherlands; 2 Div. of Clinical Immunology and Rheumatology, Academic Medical Center/University of Amsterdam, Amsterdam, The Netherlands

9.7 LOSS OF PTEN IN MYELOID CELLS CONTROLS INFLAMMATORY BONE DESTRUCTION BY REGULATING THE OSTEOCLASTOGENIC POTENTIAL OF MYELOID CELLS Stephan Blüml1, Gernot Schabbauer2, Martin Friedrich2, Antonia Puchner1, Victoria

Saferding1, Emine Sahin2, Tobias Lohmeyer2, Birgit Niederreiter1, Josef Smolen1, Kurt Redlich1

1 Division of Rheumatology, Internal Medicine III, Medical University of Vienna, Austria; 2

Institute for Vascular Biology and Thrombosis Research, Center for Biomolecular Medicine and Pharmacology, Medical University Vienna, A-1090 Vienna, Austria; 3 Institute of Immunology, Biomedical Sciences Research Center (BSRC) "Alexander; Fleming," Vari, Greece

9.8 LOW-DOSE IL-2 THERAPY SELECTIVELY EXPANDS REGULATORY T CELLS AND AMELIORATES ESTABLISHED DISEASE IN (NZBXNZW) F1 LUPUS MICE Jens Y Humrich, Lutz Kloke, Angelika Rose, Caroline von Spee, Anika Klaus, Reinmar Undeutsch, Gabriela Riemekasten University Hospital Charité, Berlin, Germany

9.9 TREATMENT WITH BGP-15, A NOVEL INSULIN SENSITIZER ATTENUATES COLLAGEN-INDUCED ARTHRITIS IN DBA/1 MICE Peter Mandl, Silvia Hayer, Stefan Blüml, Victoria Saferding, Despoina Sykoutri, Josef Smolen, Kurt Redlich Division of Rheumatology, Medical University of Vienna, Vienna, Austria

9.10 NEUTRALIZATION OF ACPA - A WAY TO GO? C Cerqueira1, E Ossipova1, M Hansson1, L Mathsson2, L Klareskog1, J Rönnelid2, and P-J Jakobsson1

1Department of Medicine, Unit of Rheumatology, Karolinska University Hospital, Stockholm, Sweden; 2Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden

9.11 NF-KAPPAB INDUCING KINASE (NIK) IS A KEY REGULATOR OF INFLAMMATION-INDUCED ANGIOGENESIS A.R. Noort1, K.P.M. van Zoest1, P. Koolwijk2, P.P. Tak1 and S.W. Tas1

1Division of Clinical Immunology & Rheumatology, Academic Medical Center, University of Amsterdam, The Netherlands; 2Department of Physiology, Institute for Cardiovascular Research (ICaR-VU), VU University Medical Center, Amsterdam, The Netherlands.

9.12 NON-CANONICAL NF-KAPPAB SIGNALING INDUCES A PROANGIOGENIC RESPONSE IN RHEUMATOID ARTHRITIS SYNOVIAL FIBROBLASTS AND ENDOTHELIAL CELLS C.X. Maracle1,2, A.R. Noort1,2, K.P.M. van Zoest1,2 and S.W. Tas1,2 1Divison of Clinical Immunology and Rheumatology, 2Department of Experimental Immunology, Academic Medical Center/University of Amsterdam, Amsterdam, Netherlands

9.13 TNF-INDUCED- PROTEIN TYROSINE PHOSPHATASE NONRECEPTOR TYPE 2 (PTPN2) AS A NEGATIVE REGULATOR OF INFLAMMATION IN RHEUMATOID ARTHRITIS Borbala Aradi1, Maria Filkova1, Stephanie Kasper2, Kerstin Klein1, Michael Scharl2, Beat A Michel1, Renate E Gay1, Edit I Buzas3, Steffen Gay1, Astrid Jüngel1

1Center of Experimental Rheumatology, University Hospital Zürich and Zürich Center for Integrative Human Physiology, Zürich, Switzerland; 2Division of Gastroenterology and Hepatology, University Hospital Zürich and Zürich Center for Integrative Human Physiology, Zürich, Switzerland; 3Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary

9.14 REGULATORY T CELL ABNORMALITIES IN PATIENTS WITH SLE SUGGEST AN IL-2-BASED IMMUNOTHERAPY C von Spee1,2, N Wassermann2, JY Humrich1,2*, G Riemekasten1,2* 1 German Rheumatism Research Centre, Berlin, Germany; 2 Charité University Hospital, Rheumatology and Clinical Immunology, Berlin, Germany; *these authors contributed equally

9.15

9.16 SYNOVIAL FIBROBLASTS FROM PATIENTS WITH RHEUMATOID ARTHRITIS DIFFERENTIATE INTO DISTINCT FIBROBLAST SUBSETS IN THE PRESENCE OF CARTILAGE Adam P. Croft1, Amy Naylor1, Birgit Zimmermann2, Debbie Hardie1, Guillaume Desanti1, Maria Jaurez1, Ulf Muller-Ladner2, Andrew Filer1, Elena Neumann2, Christopher Buckley1

1. Centre for Translational Inflammation Research, University of Birmingham, Birmingham, United Kingdom. 2. Internal Medicine and Rheumatology Justus-Liebig-University Gieβen, Kerckhoff-Klinik, Bad Nauheim, Germany.

9.17 TARGETING THE EXPRESSION OF MIR-146A IN MOUSE INFLAMMATORY LY-6CHIGH MONOCYTE SUBSET FOR THERAPEUTIC INTERVENTION IN ARTHRITIS Ammari M. 1,2, Duroux-Richard I. 1,2, Presumey J.1,2, Roubert C.3, Escriou V.4,5, Jorgensen C.1,2,6, Apparailly F.1,2,6

1Inserm, U 844, 80 rue Augustin Fliche, F-34295 Montpellier, France; 2University of Medicine, F-34000 Montpellier, France; 3Exploratory Unit, Sanofi-Aventis R & D, 371 rue du professeur J. Blayac, 34184 Montpellier, France; 4Inserm, U 1022, UFR des Sciences Pharmaceutiques et Biologiques, 4 Avenue de l'Observatoire, 75006 Paris, France; 5CNRS, UMR8151, 75006 Paris, France; 6University hospital of Montpellier, clinical unit for osteoarticular diseases, F-34295 Montpellier, France

9.18 POTENTIAL THERAPEUTIC APPLICATION OF HUMAN UMBILICAL CORD WHARTON JELLY DERIVED MESENCHYMAL STEM CELLS IN PRIMARY SJOGREN’S SYNDROME Alessia Alunno1, Pia Montanucci2, Sara Caterbi1, Onelia Bistoni1, Giuseppe Basta2, Elena Bartoloni1, Teresa Pescara2, Ilaria Pennoni2, Riccardo Calafiore1, Roberto Gerli1

1Rheumatology Unit, Department of Clinical and Experimental Medicine 2Section of Internal Medicine, Endocrine and Metabolic Sciences, University of Perugia, Perugia, Italy;

X: Others 10.1 ANALYSIS OF THE MIGRATORY POTENTIAL OF DERMAL FIBROBLASTS OF PATIENTS WITH

SYSTEMIC SCLEROSIS S Lefèvre1, FMP Meier1, A Günther2, U Müller-Ladner1, E Neumann1

1 Dept Internal Medicine and Rheumatology, Justus-Liebig-University Gießen, Kerckhoff Clinic, Bad Nauheim, Germany; 2 Dept Internal Medicine, Medical Clinic II, Justus-Liebig-University Gießen, Gießen

10.2 ANTI-AT1R AND ANTI-ETAR AUTOANTIBODIES IN PATHOGENESIS OF SYSTEMIS SCLEROSIS Angela Kill1, Mike O. Becker2, Jeannine Guenther1, Harald Heidecke3, Duska Dragun2 and Gabriela Riemekasten1

1Charité University Hospital, German Rheumatology Research Center, a Leibniz Institute, Berlin, Germany; 2Charité University Hospital, Berlin, Germany, ; 3CellTrend GmbH, Luckenwalde, Germany Presenting Author: Mislav Radic

10.3 ARTHRITIS IN PATIENTS WITH IDIOPATHIC INFLAMMATORY MYOPATHIES

Martin Klein1,2, Heřman Mann1,2, Petra HánováP1, Lenak Pleštilová1,2, Jakub Závada1,2, Jiří Vencovský1,2 1 Institute of Rheumatology, Prague, Czech Republic, 2Department of rheumatology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic

10.4 AUTOMATED AND STANDARDIZED ANALYSIS FOR HIGH DIMENSIONAL CYTOMETRIC DATA PROVIDES NEW OPTIONS FOR COMPLEX CELL-ASSOCIATED BIOMARKER SCREENING Till Sörensen1, Ursula Schulte-Wrede2, Silvia Pade1, Heike Hirseland2, Gerd Burmester1,

Andreas Radbruch2, Andreas Grützkau2, Thomas Häupl1

1Dept. of Rheumatology and Clinical Immunology, Charité University Medicine, Berlin; 2German Arthritis Research Center, Berlin

10.5 COMPARATIVE EFFECTIVENESS OF BIOLOGICAL THERAPIES IN RHEUMATOID ARTHRITIS IS INFLUENCED BY RESPONSE MEASURES AND DISEASE ACTIVITY STATE Vasco C. Romão1,2; Maria José Santos2,3; José Canas da Silva3; Joaquim Polido Pereira1,2; José Alberto Pereira da Silva1; Cátia Duarte4; José António Pereira da Silva4; Cândida Silva4; Ana Assunção Teixeira5; José Antonio Costa6; Domingos Araújo6; Fernando Pimentel Santos7; Jaime Branco7; José António Melo Gomes5; Augusto Faustino5; João Eurico Fonseca1,2; Helena Canhão1,2. 1Rheumatology Department, Lisbon Academic Medical Centre, Portugal; 2Rheumatology Research Unit, Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Lisbon, Portugal; 3Rheumatology Department, Hospital Garcia de Orta, Almada, Portugal; 4Rheumatology Department, Centro Hospitalar e Universitário de Coimbra, Coimbra, Portugal; 5Instituto Português de Reumatologia, Lisbon, Portugal; 6Rheumatology Department, Hospital Conde de Bertiandos, Unidade Local de Saúde do Alto Minho, Ponte de Lima, Portugal; 7Rheumatology Department, Hospital de Egas Moniz, Centro Hospitalar Lisboa Ocidental, Lisbon, Portugal.

10.6 DISSECTING DISEASE-SPECIFIC DIFFERENCES IN RA AND OA BY TRANSCRIPTOME ANALYSES OF SYNOVIAL TISSUE, BLOOD AND BONE MARROW MONOCYTES Biljana Smiljanovic1, Bruno Stuhlmüller1, Wlodzimierz Maslinski3, Gerd-R Burmester1, Andreas Radbruch2, Andreas Grützkau2, Thomas Häupl1 1Department of Rheumatology and Clinical Immunology, Charité CCM, Charitéplatz 1, 10117 Berlin, Germany 2German Rheumatism Research Center - Berlin (DRFZ-Berlin), Ein Leibniz-Institut, Charitéplatz 1, 10117 Berlin, Germany 3Department of Pathophysiology and Immunology, Institute of Rheumatology, Warsaw, Poland

10.7 DUAL EFFECTS OF SOLUBLE FASL AND MEMBRANE BOUND FASL ON FIBROBLAST-LIKE SYNOVIOCYTES CELLS (FLS) FROM RHEUMATOID ARTHRITIS (RA) PATIENTS Rachel Audo2, Flavia Calmon-Hamaty2, Bernard Combe1 Michael Hahne2 and Jacques Morel1,2 1Department of Rheumatology, CHU Lapeyronie, Montpellier; 2 IGMM, CNRS UMR5535, Montpellier

10.8 EVALUATION OF DISEASE ACTIVITY IN ADULT PATIENTS WITH JUVENILE IDIOPATHIC ARTHRITIS Bulina Inita1,2, Zepa Julija1,3, Astica Ineta1, Sikora Evita1, Abelite Sarmite1, Lavrentjevs Vladimirs1, Andersone Daina1,2 1Pauls Stradins Clinical University Hospital, 2University of Latvia, 3Riga Stradins University, Riga, Latvia

10.9 EVIDENCE FOR PROGRESSIVE REDUCTION AND LOSS OF TELOCYTES IN THE DERMAL CELLULAR NETWORK OF SYSTEMIC SCLEROSIS Mirko Manetti (1,2), Serena Guiducci (2), Martina Ruffo (1), Irene Rosa (1), Maria Simonetta Faussone-Pellegrini (1), Lidia Ibba-Manneschi (1), Marco Matucci-Cerinic (2)

(1)Department of Anatomy, Histology and Forensic Medicine, University of Florence, Florence, Italy; (2)Department of Biomedicine, Division of Rheumatology, AOUC, and Excellence Centre for Research, Transfer and High Education DENOthe, University of Florence, Florence, Italy

10.10 EXAMINATION OF IL-6, TNF I AND II RECEPTOR DISTRIBUTION ON PERIPHERAL BLOOD MONONUCLEAR CELLS (PBMC) IN SEROPOSITIVE AND SERONEGATIVE RHEUMATOID ARTHRITIS (RA) Martyna Skwarek, Babett Heschel, Julia Fantana, Martin Aringer

Division of Rheumatology, Department of Medicine III, University Clinical Center Carl Gustav Carus at the Technical University of Dresden

10.11 EXPRESSION OF UNFOLDED PROTEIN RESPONSE GENES IN SYNOVIUM AND BLOOD MONONUCLEAR CELLS OF HLA-B27 POSITIVE ANKYLOSING SPONDYLITIS PATIENTS IS NOT INCREASED COMPARED TO OTHER ARTHRITIS PATIENTS AND HEALTHY CONTROLS. Barbara Neerinckx, Shea Carter, Rik J Lories Laboratory of Tissue Homeostasis and Disease, Skeletal Biology and Engineering Research Center, Department of Development and Regeneration, KU Leuven, Belgium.

10.12 CHARACTERIZATION OF A NEW MPGES-1 INHIBITOR IN HUMAN AND MURINE MODELS OF INFLAMMATION

Patrick Leclerc+, Helena Idborg+, Patrick Stenberg⌘, Marina Korotkova+, Per-Johan

Jakobsson+

+Rheumatology Research Unit, CMM, Karolinska Institute, Stockholm, Sweden. ; ⌘NovaSAID

AB

10.13 IDENTIFICATION OF IMMUNOPHENOTYPIC SIGNATURES IN PERIPHERAL BLOOD OF MULTIPLE SCLEROSIS PATIENTS BY MULTIPARAMETRIC FLOW CYTOMETRY Ursula Schulte-Wrede1, Berit Rosche2, Joachim R. Grün1, Andreas Radbruch1, Andreas Grützkau1

1Deutsches Rheuma-Forschungszentrum (DRFZ), Ein Leibniz-Institut, Immune-Monitoring Core Facility, Berlin, Germany; 2Charité-Universitätsmedizin der HU-Berlin, Abteilung für Neurologie, Berlin, Germany

10.14 IDENTIFICATION OF NOVEL ACPA TARGETS IN RHEUMATOID ARTHRITIS SYNOVIAL TISSUES USING 2D GEL ELECTROPHORESIS AND MASS SPECTROMETRY E Ossipova1, G Oliynyk1,2, C Cerqueira1, S Becker3, J Ytterberg4, G Auer3, L Klareskog1, and P-J Jakobsson1. 1Department of Medicine, Unit of Rheumatology, Karolinska University Hospital, Stockholm, Sweden; 2Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden; 3Department of Oncology-Pathology, Karolinska University Hospital, Stockholm, Sweden; 4Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden

10.15 IGA RHEUMATOID FACTOR IS MORE PREDOMINANT THAN ANTI-CCP IN SUDANESE RHEUMATOID ARTHRITIS PATIENTS, WHEREAS IGG RF IS A STRONG PROGNOSTIC MARKER AND ASSOCIATED WITH EARLY ONSET. Amir I Elshafie1, 2, Sahwa Nourein1, 3, Vivek Anand Manivel1, Azita Sohrabian1, Mawahib I. E. Elidrisi4, Elnour M. Elagib4, Musa A M Nur5, Johan Rönnelid1. 1 Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden.; 2 Department of Pathology and Microbiology, Alribat University Hospital, Khartoum, Sudan.; 3 Khartoum Fertility Center, Khartoum, Sudan.; 4 Rheumatology unit, Alribat University Hospital, Khartoum, Sudan.; 5 Rheumatology unit, Military Hospital, Omdurman, Sudan.

10.16 INFLAMMATORY CYTOKINES DOWNREGULATE FOXO1 BY JNK-DEPENDENT ACCELERATION OF MRNA DEGRADATION TO PROMOTE SURVIVAL AND PROLIFERATION OF RHEUMATOID ARTHRITIS FIBROBLAST-LIKE SYNOVIOCYTES. A. M. Grabiec, C. Angiolilli, L. M. Hartkamp, L. G. M. van Baarsen, P. P. Tak, D. L. Baeten, K. A. Reedquist Department of Experimental Immunology and Department of Clinical Immunology and Rheumatology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands

10.17 INTERLEUKIN-36ALPHA IS EXPRESSED BY SYNOVIAL PLASMA CELLS AND INDUCES CYTOKINE

PRODUCTION IN HUMAN FIBROBLASTS

Silke Frey 1, Anja Derer 1, Maria-Elena Messbacher 1, Serena Bugatti 2, D. Baeten 3,

Carlomaurizio Montecucco 4, Georg Schett 1 and Axel J. Hueber 1

Department of Internal Medicine 3 and Institute for Clinical Immunology, University

hospital of Erlangen, Erlangen, Germany, 2 Division of Rheumatology, University of Pavia

School of Medicine, IRCCS Policlinico San Matteo Foundation, Pavia, Italy, 3 Academic

Medical Center, University of Amsterdam, Amsterdam, Netherlands, 4 University of Pavia

School of Medicine, IRCCS Policlinico San Matteo Foundation, Pavia, Italy

10.18 LACK OF ASSOCIATION OF SERUM INTERLEUKIN-17 AND INTERLEUKIN-23 LEVELS WITH DISEASE ACTIVITY IN PATIENTS WITH ANKYLOSING SPONDYLITIS IN LATVIA Zepa Julija1,2, Bulina Inita1,5, Nikitina-Zake Liene3, Klovins Janis3, Jurka Anita1,5, Tretjakovs Peteris1,2, Lejnieks Aivars2,4, Andersone Daina1,5 1Pauls Stradins Clinical University Hospital, 2Riga Stradins University, 3Biomedical Research and Study Centre of Latvia, 4Riga East Clinical University hospital, 5University of Latvia, Latvia

10.19 MRP8/14 SERUM COMPLEXES AS PREDICTOR OF RESPONSE TO BIOLOGICAL TREATMENTS IN RHEUMATOID ARTHRITIS Yuen Kei Choi1, Marieke J.Herenius1, Carla A. Wijbrandts1, Johannes Roth2, Dirk Föll2, Danielle M. Gerlag1, Paul-Peter Tak1,3, Dirk Holzinger2,4

1Division of Clinical Immunology and Rheumatology, Academic Medical Center/University of Amsterdam, The Netherlands; 2Institute of Immunology, University Hospital Muenster, Muenster, Germany; 3GlaxoSmithKline, Stevenage, U.K; 4Department of General Paediatrics, University Children's Hospital Muenster, Muenster, Germany

10.20 ON THE ORIGIN OF THE TYPE I INTERFERON ACTIVITY IN RHEUMATOID ARTHRITIS

T.D. de Jong1, S. Vosslamber1, M.L. Eloranta2, L. Rönnblom2, E. Mantel1, K.A. Gelderman1,

M.E. von Blomberg1, I.E. Bultink3, A.E. Voskuyl3, C.L. Verweij1,3

1Department of Pathology, VU University medical center, Amsterdam, The Netherlands; 2Department of Medical Sciences, Section of Rheumatology, Uppsala University, Sweden;

3Department of Rheumatology, VU University medical center, Amsterdam, The Netherlands

10.21 TOLL-LIKE RECEPTOR TRIGGERING OF HUMAN BASOPHILS MAY SYNERGIZE WITH IGE-MEDIATED ACTIVATION IN ACPA+ RA Jolien Suurmond,1 Jeroen N. Stoop,1 Aleida M. Bakker,1 Tom W.J. Huizinga,1 René E.M. Toes,1 and Annemie J.M. Schuerwegh1,2

1Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands; 2Department of Immunology/Allergology/Rheumatology, University of Antwerp, Antwerp, Belgium Presenting Author: Andreea Ioan-Fascinay

10.22 RESPONSE TO MTX PLUS PREDNISONE IN CAMERA II USING A MULTI-BIOMARKER DISEASE

ACTIVITY (VECTRATM DA) TEST AND DAS28-ESR

JWJ Bijlsma,1 MS Jurgens,1 JWG Jacobs,1 M Bakker,1 FPJ Lafeber,1 PMJ Welsing, 1 G Cavet,2 D

Chernoff,2 EH Sasso2 ,W Li,2 and DJ Haney2

1University Medical Center Utrecht, Utrecht, The Netherlands; 2Crescendo Bioscience, Inc.,

South San Francisco, CA, USA

10.23 STRATIFICATION OF SLE PATIENTS FOR IMPROVED DIAGNOSIS AND TREATMENT

Helena Idborg1, Stefan Rännar2, Ganna Oliynyk1, Jenny Forshed3, Rui Mamede Branca3,

Magdalena Donten2, Kate Bennett2, Johanna Gustafsson1, Anna Vikerfors1, Lennart

Truedsson4, Bo Nilsson5, Iva Gunnarsson1, Johan Trygg2,6, Janne Lehtiö3, Torbjörn

Lundstedt2,6, Elisabet Svenungsson1 and Per-Johan Jakobsson1 1 Rheumatology Unit, Department of Medicine, Karolinska Institutet and Karolinska

University Hospital, Stockholm, Sweden; 2 AcureOmics AB, Umeå, Sweden; 3 Clinical

Proteomics Mass Spectrometry, Dept. of Oncology-Pathology, Science for Life Laboratory

and Karolinska Institutet, Stockholm, Sweden; 4 Section of Microbiology, Immunology and

Glycobiology, Department of Laboratory Medicine, Lund University, Lund, Sweden; 5

Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden; 6 Computational Life Science Cluster, Department of Chemistry, Umeå University, Umeå,

Sweden; 7 Organic Pharmaceutical Chemistry BMC, Uppsala University, Uppsala, Sweden

10.24 SWITCHING INFLIXIMAB FOR NEW ALTERNATIVES: A CORRESPONDENCE ANALYSIS OF

BIOLOGICS, REASONS TO STOP, AND TIME PERIODS.

Julia Uceda1, Loreto Carmona2, Alejandro Muñoz1 , Jose L Marenco1

1Rheumatology Department, Valme University Hospital, Sevilla, Spain; 2Camilo José Cela

University, Madrid, Spain

Presenting Author: Maria Louisa Velloso

10.25 SYNOVIAL TISSUE TRANSCRIPTOME AND SYNOVIAL FLUID PROTEOME HAVE STRONGER

DISCRIMINATORY POWER THAN SERUM IN DISSECTING INFLAMMATION IN RA

Biljana Smiljanovic1, Bruno Stuhlmueller1, Silvia Pade1, Marina, Backhaus1, Gerd-R

Burmester1, Andreas Radbruch2, Andreas Grützkau2, Thomas Häupl1

1Department of Rheumatology and Clinical Immunology, Charité CCM, Charitéplatz 1,

10117 Berlin, Germany; 2German Rheumatism Research Center - Berlin (DRFZ-Berlin), Ein

Leibniz-Institut, Charitéplatz 1, 10117 Berlin, Germany

10.26 SYNOVIAL WNT AND WISP1 EXPRESSION INDUCES EXPRESSION OF CARTILAGE-DEGRADING

METALLOPROTEINASES IN THE SYNOVIUM

M. van den Bosch, A. Blom, A. van Erp, F. van de Loo, E.Blaney Davidson, P. van der Kraan,

P. van Lent, W. van den Berg

Radboud University Nijmegen Medical Centre, NCMLS, Nijmegen

10.27 SYSTEMIC SCLEROSIS – ROLE OF AGONISTIC AUTOANTIBODIES DIRECTED AGAINST THE ANGIOTENSIN RECEPTOR TYPE 1 AND THE ENDOTHELIN RECEPTOR TYPE A ON IMMUNE CELLS IN DISEASE PATHOGENESIS J Günther 1,2, A Kill 1,2, MO Becker 2, G Riemekasten 1,2

1German Rheumatism Research Centre, a Leibniz institute, 2Charité University Hospital, Berlin, Germany Presenting Author: Mislav Radic