PORTUGAL REPORT

ON THE PREVENTIVE VACCINATION PLAN FOR AVIAN

INFLUENZA IN A GAME BIRD HOLDING OF BREEDING

DUCKS (Anas Platyrhynchus)

Commission Decision 2012/110/UE

SCoFCAH Brussels, 10&11 September 2013

DSPA/ Direção Geral de Alimentação e Veterinária

History

• Emergency Vaccination Plan (EVP), approved by Decision

2008/285/EC → applied until 31 July 2008

• Preventive Vaccination Plan for 2008/2009 (PVP1), approved

by Decision 2008/838/EC → applied until 31 July 2009

• Preventive Vaccination Plan for 2010/2011 (PVP2), approved

by Decision 2010/189/EU → applied until 31 July 2011

• Preventive Vaccination Plan for 2012/2013 (PVP3), approved by Decision 2012/110/UE

→ applied until 31 July 2013

Vaccination Plan (PVP3)

• Monovalent vaccine (subtype H5)

• 50 non-vaccinated ducks (sentinels)

• Subcutaneous administration (0,5-1ml)

• Individual identification of vaccinated ducks (wing tags)

• Surveillance and monitoring of the vaccinated flocks

• Surveillance of unvaccinated poultry flocks in the surrounding area

Vaccination procedures

Vaccination Plan (PVP3)

Plan Date Vaccine Flock 4 Flock 5

PVP2 V7 02-12-2010 H5N3 1st application

PVP2 V8 17-12-2010 H5N3 2nd application

PVP3 V9 10-03-2011 H5N2 Revaccination

PVP3 V10 28-12-2011 H5N2 Revaccination

PVP3 V11 13-12-2012 H5N2 1st application

PVP3 V12 10-01-2013 H5N2 2nd application

PVP3 V13 07-06-2013 H5N2 Revaccination

Flock 4 Flock 5

Males 878 700

Females 3.508 2.800

Total 4.386 3.500

Age at 1st application 7 months 8 months

Slaughter date July 2012 July 2014

Serological Monitoring of vaccine response (PVP3)

• 20 blood samples from vaccinated ducks every month

• Antibodies titre by HI

• Positivity: titre 1:16

• All samples sent to the National Reference Laboratory

Flock 4 Flock 5

Min. <1:16 - 1:32 <1:16 - 1:256

Max. 1:32 - 1:512 1:64 - 1:1024

Mode 1:16 - 1:128 <1:16 - 1:1024

Median 1:16 - 1:64 1:16 - 1:1024

Geo.Mean 1:16 - 1:64 1:16 - 1:1024

%Positivity 70% - 100% 50% - 100%

Serological Monitoring of vaccine response (PVP3)

Serological Monitoring of vaccine response (PVP3)

15 monthly reports (behavioural and clinical surveillance)

sent by the veterinarian to the Official Services

Clinical surveillance (PVP3)

Flock Period Mortality Food

Intake Water Intake

Growth

4 Dec. 2011. to June 2012

Normal (< 0,1%) Normal Normal Normal

5 Dec. 2012 to July 2013

Normal (< 0,1%) Normal Normal Normal

Surveillance results (PVP3)

Samples Flock 4 Flock 5

Sentinels 180 180

Dead ducks 6 9

Poultry flocks in the surrounding area

35 70

Total 221 259

All results were negative

THANK YOU FOR YOUR ATTENTION