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cbPnAaospacitOntiin tissue culture techniques to become the new wave of the futurefor commercial exploitation of plants.

doi:10.1016/j.copbio.2011.05.426

130 Abstracts / Current Opinion in

ility of SMZ in water. The in vitro release of SMZ in the presence ofifferent generations of Jeffamine®cored PAMAM type dendrimersas slower than pure SMZ dissolved in methanol. Anti-bacterial

ctivity of SMZ in dendrimer solution higher than the pure SMZissolved in dimethylsulfoxide(DMSO). The results indicated that

effamine®cored PAMAM type dendrimers could be used for drugelivery applications under suitable conditions.

oi:10.1016/j.copbio.2011.05.423

22

nhibition of lipid peroxidation and phenolic contents ofypericum helianthemoides extract

oheila Moein 1, Zahra Sabahi 2, Mahmood Reza Moein 2, Fatemeharmani 2

Department of Biochemistry and Research Center for Molecularedicine, Faculty of Medicine, Hormozgan University of Medical Sci-

nces, Bandar Abbas, IranDepartment of Pharmacognosy and Medicinal Plants Research Cen-

er, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz,ran

-mail address: Soheila 9@Yahoo.Com (S. Moein)

Today the antioxidant properties of plants with fewer sideffects were interested. In this research, the antioxidant activitiesf Hypericum extract by lipid peroxidation, phenolic determinationnd frap method were evaluated. The plant was collected from Farsrovince and plant extraction was obtained using ethanol. Lipideroxidation was done using ferric thiocyanate method, pheno-

ic compounds determined by Folin–Ciocalteu reagent and in frapethod TPTZ was used. Inhibition of lipid peroxidation of Hyper-

cum extract, BHT, Vit C were 59.17%, 66.9% and 64.06% respectivelyn tenth day. The amount of phenolic compounds in Hypericumxtract was 0.02 mg/g as gallic acid equivalents (GAE). In Frapethod, the reduction potential of extract was higher than con-

rol (which had not plant extract). Hypericum extract possessedntioxidant activity specially inhibition of lipid peroxidation.

oi:10.1016/j.copbio.2011.05.424

lant Biotechnology Section

1

lant responses to abiotic stress

onica Boscaiu 1, Inmaculada Bautista 2, Pilar Donat 3, Antonioidon 2, Josep Llinares 3, Cristina Lull 2, Olga Mayoral 3, Oscaricente 4

Instituto Agroforestal Mediterraneo (IAM), SpainGrupo RE-FOREST, Dpto. de Ingenieria Hidraulica y Medio Ambiente,painInstituto de Investigacion para la Gestion Integral de Zonas Costeras

IGIC), SpainInstituto de Biologia Molecular y Celular de Plantas (IBMCP, UPV-SIC), Universidad Politecnica de Valencia, Valencia, Spain

-mail address: ovicente@ibmcp.upv.es (O. Vicente)

The study of plant responses to abiotic stress and stress toler-

nce mechanisms is a very active field in plant biology research,ue to its unquestionable academic interest, but also because abi-tic stress conditions (mostly drought and high soil salinity) are

hnology 22S (2011) S15–S152

he major causes reducing crop yields worldwide. Several con-erved mechanisms of response have been defined at the cellularevel, based on the control of ion homeostasis, the maintenance ofsmotic balance, the synthesis of ‘protective’ metabolites and pro-eins, or the activation of antioxidant systems. Paradoxically, mostf these studies have been performed using stress-sensitive modelpecies — such as Arabidopsis thaliana — under laboratory or green-ouse artificial conditions. As a complementary approach, we are

nvestigating the mechanisms of tolerance in wild plants adaptedo stress in their natural habitats. For this, we are determining theevels of different biochemical markers of specific stress responseathways (accumulation of ions, osmolytes, chemical antioxidants,tc.) in plants growing in the field, with the aim to correlate theirpatial and temporal patterns of variation with the specific environ-ental conditions — soil properties, climatic parameters — of the

lants.Funded by the Spanish Ministry of Science and Innovation

project CGL2008-00438/BOS), with contribution from the ERDF.

oi:10.1016/j.copbio.2011.05.425

2

lant tissue culture techniques—Tools in plant micro-ropagation

hmet Onay 1, Hakan Yildirim 2, Yelda Ozden Tokatli 3, Hulyakdemir 3, Veysel Suzerer 3

Department of Biology, Faculty of Science, University of Dicle,iyarbakir, TurkeyDepartment of Horticulture, Faculty of Agriculture, University oficle, Diyarbakir, TurkeyDepartment of Molecular Biology and Genetics, Gebze Institute ofechnology, Kocaeli, Turkey

-mail address: ahmeto@dicle.edu.tr (A. Onay)

In this study, we will review the principles underlying theoncepts of plant tissue culture techniques which deal with theuilding-blocks of agriculture, horticulture, and the food industries.lant tissue culture techniques are one of the important biotech-ological tools that have found wide application around the world.lthough numerous strides have been accomplished with fruit,gronomic and vegetable species and cultivars, this study will focusn the tissue culture techniques of pistachio and almond as modelystem. Already, commercially important applications of micro-ropagation such as organogenesis (the induction of axillary anddventitious shoots, temporary immersion systems (TIS) and thinell layer (TCL) technology), somatic embryogenesis, micrograft-ng of vegetables, fruits and ornamentals have begun to appear inhe market-places of America, Japan and some European countries.ur work of over 20 years experience will be presented in relationot only to past accomplishments, but also with presenting newerechnologies and developments that may provide some challeng-ng ideas to both newcomers and specialists, and even old hands