Plant Pathology Laboratory Outline

7/30/2019 Plant Pathology Laboratory Outline

1/7

PLP 3002/5005 Fundamentals of Plant Pathology/General Plant Pathology(Fall 2011)

COURSE AND LABORATORY INSTRUCTOR: Dr. Brantlee Spakes RichterOffice: 2519 Fifield Hall

Phone: 352-273-2014Email: [email protected] Hours: Monday & Thursday 2:00-4:00pm, or by appointment

TEACHING ASSISTANT:Office: 2509 Fifield HallPhone: 352-870-5577Email: [email protected] Hours: by appointment

COURSE(S): Fundamentals of Plant Pathology (PLP 3002C, sections 3931 and 3933) and GeneralPlant Pathology (PLP 5005C, sections 3937 and 3938) are taught concurrently. Undergraduates are

encouraged to enroll in PLP 3002, whereas Plant Pathology graduate students and DPM students areencouraged to enroll in PLP 5005 so that they can receive graduate credits for the class. Studentsenrolled in PLP 5005 will be required to complete two additional projects beyond the requirements ofPLP3002 (described below).

PREREQUISITE: BOT 2010 or BSC 2010

CREDITS: 4

CLASS TIME/LOCATION:Lectures: 2316 Fifield Hall, Tuesday and Thursday period 4 @ 10:40-11:30 amLaboratories: 2306 Fifield Hall, Tuesday (sec. 3931/3937) orWednesday (sec. 3938/6959)

Periods 6-8 @ 12:50-3:50 pm

INTRODUCTION: Plant pathology is the science of plant diseases, the microorganisms that cause them,and their interactions. The ultimate goal of plant pathologists is to reduce the losses caused by plantdiseases, thereby increasing plant quality and quantity. Plant diseases are caused by many of the sametypes of organisms that cause diseases in animals and humans and, as such, many of the principles thatapply to animal and human medicine apply to plant diseases. The objective of this course is to introducestudents to the many different types of plant pathogens, their basic biology, examples of the types ofdisease they cause, and the basic principles and concepts of their development, spread, andmanagement.

TEXT: Plant Pathology 5th Ed. (2005) by G. N. Agrios, Elsevier Academic Press, Inc. (The 4th

edition ofthis book is also acceptable; published by Academic Press, Inc. NY.)

ATTENDANCE: Attendance is not mandatory, but is highly advisable. You are expected to participate in

every class and laboratory, and there will be daily quizzes and/or class activities which will contribute toyour grade in the class. Missed activity grades may only be made up for excused absences, at thediscretion of the instructor. Absences due to personal planning (ie, planning to be somewhere other thanclass during class time) will not be excused, and missed points may not be made up. There will be nomake-up lab sessions.

LABORATORY: The laboratory will emphasize principles and concepts of plant pathology throughdemonstrations and hands-on exercises using living organisms and prepared specimens. Labs willtypically include an introduction to the lab exercise, work with demonstration materials, and a deliverable


2/7

to be handed in either at the end of the session or the beginning of the subsequent session. It is advisedthat you keep a lab notebook for all labs. Your lab notebook will not be graded, but a well-kept notebookwill be highly beneficial to you in preparing lab assignments and studying for exams.

PLP 5005 Requirements: Students taking the class for graduate credit will have one individual and onegroup assignment, in addition to the set of requirements for the undergraduate course:

1. Each student will be required to select a set of 2-3 journal articles on a single topic, and to write acritique of the selected articles. Articles must be published in international peer-reviewedjournals, and should be selected to present contrasting approaches to a single research question.Your critique will focus on the differing approaches that each research lab takes, discuss theadvantages and disadvantages of each set of methods used, and make recommendations forfuture research on the topic.

2. Graduate students as a group will conduct an experiment on the topic of their choosing (subjectto instructor approval). All students may work together, or you may split into no more than twogroups, to accommodate schedules and topical areas of interest. There will be three deliverablesassociated with the group project: (1) a research proposal, (2) a journal-style article, and (3) anin-class presentation.

Topics selected for individual assignments may be used as a starting point for the group experiment(s).

Further details on both assignments will be discussed in class.

EXAMS AND GRADING: There will be three lecture exams (two mid-terms and one final) and onelaboratory exam. The final lecture examination (Exam Group 12B) is scheduled for 10:00 AM 12:00 PM,Monday, December 12, in room 2316 Fifield. The lab exam will be held during the last regularlyscheduled laboratory session, December 6 and 7.

Grading for PLP3002: There will be a total of 500 possible points earned for PLP3002 students, with 100points from each of the exams (3 lecture and 1 lab), and 50 points each from class and labparticipation assignments/quizzes.

Grading for PLP5005: Graduate students will be responsible for the same 500 points, plus two additionalassignments worth 150 points, for a total of 650 possible points.

Component Points PLP3002 PLP5005Exam 1 100 20% 15.4%

Exam 2 100 20% 15.4%

Final Exam 100 20% 15.4%

Class Participation 50 10% 7.7%

Lab Assignments 50 10% 7.7%

Lab Exam 100 20% 15.4%

Critique Paper 50 7.7%

Group Project 100 15.4%

ACADEMIC HONESTY

In 1995 the UF student body enacted a new honor code and voluntarily committed itself to the higheststandards of honesty and integrity. When students enroll at the university, they commit themselves to thestandard drafted and enacted by students. In adopting this honor code, the students of the University ofFlorida recognize that academic honesty and integrity are fundamental values of the universitycommunity. Students who enroll at the university commit to holding themselves and their peers to thehigh standard of honor required by the honor code. Any individual who becomes aware of a violation ofthe honor code is bound by honor to take corrective action. The quality of a University of Floridaeducation is dependent upon community acceptance and enforcement of the honor code. The HonorCode: We, the members of the University of Florida community, pledge to hold ourselves and ourpeers to the highest standards of honesty and integrity. On all work submitted for credit by students


3/7

at the university, the following pledge is either required or implied: On my honor, I have neither givennor received unauthorized aid in doing this assignment. The university requires all members of itscommunity to be honest in all endeavors. A fundamental principle is that the whole process of learningand pursuit of knowledge is diminished by cheating, plagiarism and other acts of academic dishonesty. Inaddition, every dishonest act in the academic environment affects other students adversely, from theskewing of the grading curve to giving unfair advantage for honors or for professional or graduate schooladmission. Therefore, the university will take severe action against dishonest students. Similarly,measures will be taken against faculty, staff and administrators who practice dishonest or demeaningbehavior. Students should report any condition that facilitates dishonesty to the instructor, departmentchair, college dean or Student Honor Court. (Source: 2007-2008 Undergraduate Catalog) It is assumedall work will be completed independently unless the assignment is defined as a group project, in writing bythe instructor. This policy will be vigorously upheld at all times in this course.

SOFTWARE USEAll faculty, staff and students of the university are required and expected to obey the laws and legalagreements governing software use. Failure to do so can lead to monetary damages and/or criminalpenalties for the individual violator. Because such violations are also against university policies and rules,disciplinary action will be taken as appropriate.

CAMPUS HELPING RESOURCES

Students experiencing crises or personal problems that interfere with their general wellbeing areencouraged to utilize the universitys counseling resources. Both the Counseling Center and StudentMental Health Services provide confidential counseling services at no cost for currently enrolled students.Resources are available on campus for students having personal problems or lacking clear career oracademic goals, which interfere with their academic performance. The Counseling Center is located at301 Peabody Hall (next to Criser Hall). Student Mental Health Services is located on the second floor ofthe Student Health Care Center in the Infirmary.

University Counseling Center, 301 Peabody Hall, 392-1575,www.counsel.ufl.edu Career Resource Center, CR-100 JWRU, 392-1602, www.crc.ufl.edu/ Student Mental Health Services, Rm. 245 Student Health Care Center, 392-1171,

www.shcc.ufl.edu/smhs/- Alcohol and Substance Abuse Program (ASAP)

- Center for Sexual Assault / Abuse Recovery & Education (CARE)- Eating Disorders Program- Employee Assistance Program- Suicide Prevention Program

STUDENTS WITH DISABILITIESThe Disability Resource Center coordinates the needed accommodations of students with disabilities.This includes registering disabilities, recommending academic accommodations within the classroom,accessing special adaptive computer equipment, providing interpretation services and mediating faculty-student disability related issues. 0001 Reid Hall, 392-8565, www.dso.ufl.edu/drc/THE INSTRUCTOR RESERVES THE RIGHT TO CHANGE OR MODIFY INFORMATION PROVIDED IN

THE SYLLABUS. CLASS ANNOUNCEMENTS SUPERSEDE SYLLABUS STATEMENTS


4/7

COURSE LECTURE SCHEDULE Fall 2011(Numbers refer to chapter and page no. of the reading assignment

in Agrios 5th

edition text)

Date Topic

Aug 23: Introduction to Plant Pathology, terminologyChapt 1: 3-27, Chapt 1: 71-75

Aug 23: (12.50-1.30 pm)Abiotic diseases, Chapt 10: 357-383

Aug 25: Introduction to Plant Pathology, diagnosisSeparate handout

Aug 30: Introduction to Plant Pathology, historyChapt 1: 29-53 and 65-69

Sept 01: Introduction to Plant Pathology, disease development and cyclesChapt 2: 77-89 and 96-102

Sept 06: Plant Pathogenic Fungi/Diseases caused by fungiChapt 11: 383-404



Sept 15: Plant Pathogenic Bacteria/Diseases caused by bacteria

Chapt 12: 615-627 (Jones)

Sept 20: Plant Pathogenic Bacteria/Diseases caused by bacteriaChapt 12: 615-627 (Jones)

Sept 22: Plant Pathogenic Bacteria/Diseases caused by bacteriaChapt 12: 615-627 (Jones)

Sept 27: Plant Pathogenic Viruses/Diseases caused by virusesChapt 14: 724-756

Sept 29: Plant Pathogenic Viruses/Diseases caused by virusesChapt 14: 724-756

Oct 04: Plant Pathogenic Viruses/Diseases caused by virusesChapt 14: 724-756

Oct 06: Plant Pathogenic NematodesChapt 15: 826-836 (Crow)

Oct 11: Plant Pathogenic NematodesChapt 15: 826-836 (Crow)


5/7

Oct 13: Effects of pathogens on plant physiologyChapt. 3: 106-121

Oct 18: How pathogens attack plantsChapt 5:176-203

Oct 20: Genetics of Plant diseaseChapt 4: 125-174

Oct 25: Genetics of Plant diseaseChapt 4: 125-174

Oct 27: Plant defenses - structuralChapt 6:210-217

Nov 1: Plant defenses - biochemicalChapt 6:217-236

Nov 3: Midterm examEnvironmental factors that affect infectious diseases

Chapt 7: 249-265

Nov 8: Plant disease epidemiologyChapt 8: 266-289

Nov 10: Chemical control of plant diseasesChapt 9: 295-348

Nov 15: Cultural control of plant diseasesChapt 9: 295-348

Nov 17: Forum discussion about careers in Plant PathologyChapt 1: 60-65

Nov 22: Biocontrol of plant diseasesChapt 9: 295-348

Nov 24: No class (Thanksgiving Week)

Nov 29: Integrated approach to vegetable disease management

Dec 01: Presentations of class projects by grad students

Dec 06: last class period

Dec 12: 10:00AM-12:00PM final exam in 2316


6/7

PLP 3002/5005

LABORATORY SCHEDULEFall 2011

(Numbers in parentheses are pages in Agrios 5th

edition)

Aug 23-24: Abiotic diseases lecture McGovernIntroduction to PLP Lab and literature van BruggenTour on campus, microscope use, moist chamberSymptoms and Signs of Plant Diseases (397-402)

Aug 30-31: Isolations of bacteria and fungi van BruggenGoing through a simple key

Sept 6-7: Soilborne diseases by fungi (404-421; 522-552; 593-603): Rollins, BennyMicroscopic observationsOomycota and Myxomycota (Phytophthora, Pythium, Plasmodiophora)Ascomycetes and Basidiomycetes (Fusarium, Verticillium, Sclerotium,Rhizoctonia)

Sept 13-14: Foliar diseases by fungi (439-445; 448-472; 483-500; 501-519) Rollins, BennyMacroscopic and microscopic observationsOomycota (Phytophthora, Peronospora)Ascomycetes (Venturia, Guignardia, Colletotrichum, Botrytis,Erisyphe; Alternaria)

Sept 20-21: Bacterial diseases (616-675) JonesMicrocope (streaming, Gram staining, KOH)(Erwinia, Pseudomonas,Xanthomonas, Agrobacterium, Ralstonia)

Sept 27-28: Foliar diseases by fungi (445-447; 562-593) RollinsMacroscopic and microscopic observations

Basidiomycota (Puccinia,Ustilago, Tilletia, Taphrina)

Oct 4-5: Plant Viruses inoculation of plant viruses Polston, CapobiancoI. Mechanical inoculationII. Insect inoculation aphid inoculation of potyviruses

Oct 11-12: Nematodes (overview 826-837, 838-870) CrowMicroscopic observations, demonstration of isolation techniques(Meloidogyne, Heterodera,Pratylenchus, Radopholus)(Trichodorus,Aphelenchoides)

Oct 18-19: Plant Viruses virus symptoms Polston, CapobioancoI. Examine results from previous laboratoryII. Role of symptoms in diagnosis of viral diseases

Oct 25-26 : Intro molecular techniques 1 (protein-protein interaction assays) Song

Nov 1-2 Intro molecular techniques 2 (polymerase chain reaction, Songrolling circle amplification)


7/7

Nov 9-10 Postharvest diseases (434-438; 507-510; 546-561; 656-662) Bartz, SpicelandMacroscopic and microscopic observations(Rhizopus, Mucor, Monilia,Aspergillus,Penicillium, Sclerotinia,Erwinia)

Nov 15-16: Last lab period

Nov 22-23: No lab (Thanksgiving week)

Nov 29-30: Lab review

Dec 6: Lab exam in Room 2306, 12:50 AM to 3.50 PMDec 7: Lab exam in Room 2306, 12:50 AM to 3.50 PM

Documents

Plant Pathology Laboratory Outline