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JNC CORPORATION
pKAZ-412 Cat. No. P-011
Gene/Insert name: 19 kDa protein of Oplophorus Luciferase (KAZ): catalytic component (O. gracilorostris)
Vector backbone: pBluescript SK Vector type: E. coli Backbone size w/o insert (bp): 2,958 Bacterial resistance: Ampicillin Growth strain: JM83 Growth temperature (oC): 37 Growth instructions: pKAZ-412 is resistant to ampicillin (50 µg/mL) High or low copy: High copy Vector map: pKAZ-412 Coding sequence: Nucleotide sequence & Amino acid sequence Plasmid sequence: pKAZ-412 (3,846 bp) Restriction enzyme list: Restriction enzyme sites of pKAZ-412 GenBank Accession No.: AB030246 Size: 10 µg Constituents: 10 mM Tris-HCl (pH 7.6)－1 mM EDTA Terms and Licenses: MTA
Laboratory Reagent For Research Use Only
Feature for pKAZ-412:Sourse Comments1-331 1-230
Residue 1-331 1-230 385-972 1-588
pBluescript SK backbone lac promoter/operator
1,214-3,846 326-2,958 pBluescript SK backbone
3,673 2,785 ori: Origin of replication2,045-2,905 1,157-2,017 Ampr: Ampicillin resistance gene
JNC Corporation, Yokohama Research Center
Name: pKAZ-412Insert: 19 kDa protein of Oplophorus luciferase (KAZ) cDNA Catalytic component (O. gracilorostris)Vector: pBluescript SK
Ref.1)
M A Y S - - - I L A ***DNA fragment:
19 kDa Protein of Oplophorus Luciferase, KAZ
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JNC CORPORATION
Cat. No. P-011
1,476-1,782 588-894 f1(+) origin
KAZ cDNA
PstINotI
NotI
NotI
HindIII
HindIII
EcoRI
EcoRI
XhoI
XhoI
f1(+) origin
Ampr
pKAZ-412(3,846 bp)
orilac promoter/operator
ATG-GCG-TAC-TCC-.......-ATT-CTT-GCC-TAA
KAZ amino acid seq. & DNA seq.: GenBank Accession No. AB030246 Inouye, S. et al. FEBS Lett. (2000) 481: 19-25.
19 kDa protein ORF
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JNC CORPORATION
Cat. No. P-011 Gene coding region (ORF: 19 kDa protein/KAZ)
Nucleotide sequence GAATTCGCGGCCGCTGTTTGGGTTATAGGTGGTATATCATTAACTCTACTTGAGAGAAGATGGCGTACTCCACTCTGTTCATAATTGCATTGACCGCCGTTGTCACTCAAGCTTCCTCAACTCAAAAATCTAACCTAACTTTTACGTTGGCAGATTTCGTTGGAGACTGGCAACAGACAGCTGGATACAACCAAGATCAAGTGTTAGAACAAGGAGGATTGTCTAGTCTGTTCCAAGCCCTGGGAGTGTCAGTCACGCCCATACAGAAAGTTGTACTGTCTGGGGAGAATGGGTTAAAAGCTGATATTCATGTCATAATACCTTACGAGGGACTCAGTGGTTTTCAAATGGGTCTAATTGAAATGATCTTCAAAGTTGTTTACCCCGTGGATGATCATCATTTCAAGATTATTCTCCATTATGGTACACTCGTTATTGACGGTGTAACACCCAACATGATTGACTACTTTGGAAGACCTTACCCTGGAATTGCTGTATTTGACGGCAAGCAGATCACAGTTACTGGAACTCTGTGGAACGGCAACAAGATCTATGATGAGAGGCTAATCAACCCTGATGGTTCACTCCTCTTCAGAGTTACTATCAATGGAGTCACGGGATGGAGGCTTTGCGAGAACATTCTTGCCTAAATTACATCTCGAGAATTGCTTAAAGCCTTTTTATGTCTATAAATTGGAGTGGAAAATGTATAATACATATGATTTTTAGGACAGTTATTTTATTTAATTGCTCACTTAAATTTAAATCTGAAGACCACTATAACTGTTCAGAATGGAACTGTAGTCAAACTGTATTAAATGCATTAAAGATCTTATCATATGATTTAGAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAGCGGCCGCGAATTC
Amino acid sequence *EKMAYSTLFIIALTAVVTQASSTQKSNLTFTLADFVGDWQQTAGYNQDQVLEQGGLSSLFQALGVSVTPIQKVVLSGENGLKADIHVIIPYEGLSGFQMGLIEMIFKVVYPVDDHHFKIILHYGTLVIDGVTPNMIDYFGRPYPGIAVFDGKQITVTGTLWNGNKIYDERLINPDGSLLFRVTINGVTGWRLCENILA*
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JNC CORPORATION
Cat. No. P-011pKAZ-412 (3,846 bp)
GCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTCGAAATTAACCCTCACTAAAGGGAACAAAAGCTGGAGCTCCACCGCGGTGGCGGCCGCTCTAGAACTAGTGGATCCCCCGGGCTGCAGGAATTCGCGGCCGCTGTTTGGGTTATAGGTGGTATATCATTAACTCTACTTGAGAGAAGATGGCGTACTCCACTCTGTTCATAATTGCATTGACCGCCGTTGTCACTCAAGCTTCCTCAACTCAAAAATCTAACCTAACTTTTACGTTGGCAGATTTCGTTGGAGACTGGCAACAGACAGCTGGATACAACCAAGATCAAGTGTTAGAACAAGGAGGATTGTCTAGTCTGTTCCAAGCCCTGGGAGTGTCAGTCACGCCCATACAGAAAGTTGTACTGTCTGGGGAGAATGGGTTAAAAGCTGATATTCATGTCATAATACCTTACGAGGGACTCAGTGGTTTTCAAATGGGTCTAATTGAAATGATCTTCAAAGTTGTTTACCCCGTGGATGATCATCATTTCAAGATTATTCTCCATTATGGTACACTCGTTATTGACGGTGTAACACCCAACATGATTGACTACTTTGGAAGACCTTACCCTGGAATTGCTGTATTTGACGGCAAGCAGATCACAGTTACTGGAACTCTGTGGAACGGCAACAAGATCTATGATGAGAGGCTAATCAACCCTGATGGTTCACTCCTCTTCAGAGTTACTATCAATGGAGTCACGGGATGGAGGCTTTGCGAGAACATTCTTGCCTAAATTACATCTCGAGAATTGCTTAAAGCCTTTTTATGTCTATAAATTGGAGTGGAAAATGTATAATACATATGATTTTTAGGACAGTTATTTTATTTAATTGCTCACTTAAATTTAAATCTGAAGACCACTATAACTGTTCAGAATGGAACTGTAGTCAAACTGTATTAAATGCATTAAAGATCTTATCATATGATTTAGAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAGCGGCCGCGAATTCGATATCAAGCTTATCGATACCGTCGACCTCGAGGGGGGGCCCGGTACCCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGA
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JNC CORPORATION
Residue Sourse Comments1-331 1-331 pBluescript SK backbone 1-230 1-230 lac promoter/operator 385-972 1-588 19 kDa protein ORF 1,214-3,846 326-2,958 pBluescript SK backbone 1,476-1,782 588-894 f1(+) origin 3,673 2,785 ori: Origin of replication 2,045-2,905 1,157-2,017 Ampr: Ampicillin resistance gene
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JNC CORPORATION
Cat. No. P-011Restriction enzyme sites of pKAZ-412
Enzyme Name Sequence Count Cutting Positions AccI GT!MKAC 1 1243ApaI GGGCC!C 1 1261Asp718I G!GTACC 1 1263BamHI G!GATCC 1 309BclI T!GATCA 1 718BglII A!GATCT 2 873, 1154EcoRI G!AATTC 2 327, 1215EcoRV GAT!ATC 1 1223HincII GTY!RAC 1 1244HindIII A!AGCTT 2 435, 1227KpnI GGTAC!C 1 1267MluI A!CGCGT 0 -NcoI C!CATGG 0 -NdeI CA!TATG 2 1043, 1164NheI G!CTAGC 0 -NotI GC!GGCCGC 3 290, 334, 1208 PstI CTGCA!G 1 325SacI GAGCT!C 1 277SalI G!TCGAC 1 1242ScaI AGT!ACT 1 2352SmaI CCC!GGG 1 317StuI AGG!CCT 0 -XbaI T!CTAGA 1 297XhoI C!TCGAG 2 984, 1248
JNC Corporation, Yokohama Research Center
5-1 Okawa, Kanazawa-ku, Yokohama, Japan 236-8605Tel: 045-786-5501 Fax: 045-786-5511 E-mail: [email protected]
Contact usSupplier
Shin Otemachi Bldg. 9F 2-2-1 Otemachi, Chiyoda-ku, Tokyo 100-8105 URL http://www.jnc-corp.co.jp
JNC CORPORATION
SAFETY DATA SHEETAccording to JIS Z 7253:2012
Creation Date 01-Aug-2016
Revision Date
Section 1: PRODUCT AND COMPANY IDENTIFICATION
Product name pKAZ-412Product code P-011Recommended uses and restrictions on use
For research purposes
Manufacturer
Supplier
Emergency telephone number
JNC CORPORATION,Yokohama research Center5-1, Okawa, Kanazawa-ku, Yokohama 236-8605, Japan Phone: +81-45-786-5501Fax: +81-45-786-5511JNC CORPORATIONShin Otemachi Bldg., 2-2-1 Otemachi, Chiyoda-ku, Tokyo 100-8105Phone: +81-3-3243-6330Fax: +81-3-3243-6363+81-45-786-5501
Section 2: HAZARDS IDENTIFICATION
GHS classificationClassification of the substance or mixture
･ Not a hazardous substance or mixture according to the Globally Harmonized System (GHS)
Pictograms
Signal word･ none
Hazard statements･ Not a hazardous substance or mixture according to the Globally Harmonized System (GHS)
Precautionary statements-(Prevention)
• Not applicablePrecautionary statements-(Response)
• Not applicablePrecautionary statements-(Storage)• Not applicable
Precautionary statements-(Disposal)
• Not applicable
Others Other hazards
･ Not available
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Section 3: COMPOSITION/INFORMATION ON INGREDIENTS
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Mixture
CPS-R-0040Y pKAZ-412
･ none
Chemical Name Weight-% Molecular weight EC No. ISHL No. CAS No.EDTA <0.1% 292.24 200-449-4 N/A 60-00-4
Creation Date 01-Aug-2016 _____________________________________________________________________________________________
Section 4: FIRST AID MEASURES
InhalationRemove to fresh air. If symptoms persist, call a physician.
Skin contactWash off immediately with soap and plenty of water. If symptoms persist, call a physician.
Eye contactIF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. Immediate medical attention is required.
IngestionRinse mouth. Never give anything by mouth to an unconscious person. Call a physician or poison control center immediately.Do not induce vomiting without medical advice.
Protection of first-aidersUse personal protective equipment as required.
Section 5: FIRE FIGHTING MEASURES
Suitable extinguishing mediaWater spray (fog), carbon dioxide (CO2), Foam, Extinguishing powder, Sand
Unsuitable extinguishing mediaNo information available
Special extinguishing methodNo information available
Specific hazards arising from the chemical productThermal decomposition can lead to release of irritating and toxic gases and vapors.
Protection of fire-fightersUse personal protective equipment as required. Firefighters should wear self-contained breathing apparatus and full firefightingturnout gear.
Section 6: ACCIDENTAL RELEASE MEASURES
Personal precautions, protective equipment and emergency proceduresFor indoor, provide adequate ventilation process until the end of working. Deny unnecessary entry other than the peopleinvolved by, for example, using a rope. While working, wear appropriate protective equipments to avoid adhering it on skin, orinhaling the gas. Work from windward, and retract the people downwind.
Environmental precautionsTo be careful not discharged to the environment without being properly handled waste water contaminated.
Methods and materials for contaminent and methods and materials for cleaning up
_____________________________________________________________________________________________
Do not touch spilled material without suitable protection(See section 8). After material is completely picked up, wash the spill site with soap and water and ventilate the area. Put all wastes in a plastic bag for disposal and seal it tightly. Remove, clean, or dispose of contaminated clothing.
Recoverly, neutralization
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No information availableSecondary disaster prevention measures
Clean contaminated objects and areas thoroughly observing environmental regulations.
CPS-R-0040Y pKAZ-412
Section 7: HANDLING AND STORAGE
Handling Technical measures
Avoid contact with strong oxidizing agents. Use with local exhaust ventilation.Precautions
Creation Date 01-Aug-2016 _____________________________________________________________________________________________
Do not rough handling containers, such as upsetting, falling, giving a shock, and dragging. Prevent leakage, overflow, andscattering. Not to generate steam and dust in vain. Seal the container after use. After handling, wash hands and face, and thengargle. In places other than those specified, should not be smoking or eating and drinking. Should not be brought contaminatedprotective equipment and gloves to rest stops. Deny unnecessary entry of non-emergency personnel to the handling area.
Safety handling precautionsAvoid contact with skin, eyes or clothing. Use personal protective equipment as required.
Storage Safe storage conditions
Storage conditions Container protected from light, and store tightly closed in freezer (-20°C).
Safe packaging materialIncompatible substances
Glass or Plastic containerStrong oxidizing agents
Section 8: EXPOSURE CONTROLS/PERSONAL PROTECTION
Engineering controlsIn case of indoor workplace, seal the source or use a local exhaust system. Provide the safety shower facility, and hand- andeye-wash facility. And display their position clearly.
Exposure limits
This product, as supplied, does not contain any hazardous materials with occupational exposure limits established by the region specific regulatory bodies.
Personal protective equipmentDust maskProtection glovesprotective eyeglasses or chemical safety goggles
Respiratory protection Hand protectionEye protectionSkin and body protection Long-sleeved work clothes
General hygiene considerationsHandle in accordance with good industrial hygiene and safety practice.
Section 9: PHYSICAL AND CHEMICAL PROPERTIES
Form
AppearanceColorless
Odor No data availablepH No data availableMelting point/freezing point No data availableBoiling point, initial boiling point and boiling range No data availableFlash point No data availableEvaporation rate: No data availableFlammability (solid, gas): No data availableUpper/lower flammability orexplosive limits
Color
Lower：：：：Vapour pressure
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Vapour densitySpecific Gravity / Relative density
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Solubilitiesn-Octanol/water partition coefficient:(log Pow)Auto-ignition temperature:
CPS-R-0040Y pKAZ-412
Decomposition temperature:
No data availableNo data available No data available No data available No data available Water: soluble No data available No data available No data available
No data available
Viscosity (coefficient of viscosity)Dynamic viscosity
No data availableNo data available
Creation Date 01-Aug-2016 _____________________________________________________________________________________________
Section 10: STABILITY AND REACTIVITY
Stability
Stability Stable under the recommendated strage conditionReactivity No data available
Hazardous reactionsNone under normal processing
Conditions to avoidExtremes of temperature and direct sunlight
Incompatible materialsStrong oxidizing agents
Hazardous decomposition productsCarbon monooxide (CO), carbon dioxide (CO2), Nitrogen oxides (NOx)
Section 11: TOXICOLOGICAL INFORMATION
Acute toxicity No data available
Skin irritation/corrosion No data availableSerious eye damage/ irritation No data availableRespiratory or skin sensitization No data availableReproductive cell mutagenicity No data availableCarcinogenicity No data available
Reproductive toxicity No data availableSTOT-single exposure No data availableSTOT-repeated exposure No data availableAspiration hazard No data available
Section 12: ECOLOGICAL INFORMATION
Ecotoxicity No information available
Other data No data available
Persistence and degradability No information availableBioaccumulative potential No information availableMobility in soil No information availableHazard to the ozone layer No information available
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Section 13: DISPOSAL CONSIDERATIONS
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Waste from residuesDisposal should be in accordance with applicable regional, national and local laws and regulations.
CPS-R-0040Y
Contaminated container and contaminated packaging
pKAZ-412
Disposal should be in accordance with applicable regional, national and local laws and regulations.
Creation Date 01-Aug-2016 _____________________________________________________________________________________________
Section 14: TRANSPORT INFORMATION
ADR/RID Not regulatedUN number -Proper shipping name:UN classficationSubsidiary hazard classPacking groupMarine pollutant Not applicable
IMDG Not regulatedUN number -Proper shipping name:UN classficationSubsidiary hazard classPacking groupMarine pollutant (Sea) Not applicableTransport in bulk according toAnnex II of MARPOL 73/78 andthe IBC Code
No information available
IATA Not regulatedUN number -Proper shipping name:UN classficationSubsidiary hazard classPacking groupEnvironmentally HazardousSubstance
Not applicable
Section 15: REGULATORY INFORMATION
International Inventories EINECS/ELINCS -TSCA -
Japanese regulations Fire Service Act Not applicablePoisonous and DeleteriousSubstances Control Law
Not applicable
Industrial Safety and Health Act Not applicableAct on the Evaluation ofChemical Substances andRegulation of Their Manufacture,etc
Not applicable
Regulations for the carriage andstorage of dangerous goods inship
Not applicable
Civil Aeronautics Law Not applicable
_____________________________________________________________________________________________
Pollutant Release and TransferRegister Law
Not applicable
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Export Trade Control Order Not applicable
Section 16: OTHER INFORMATION
CPS-R-0040Y pKAZ-412
Literature and references
Creation Date 01-Aug-2016 _____________________________________________________________________________________________
DisclaimerThe information provided in this Safety Data Sheet is correct to the best of our knowledge, information and belief at the date of itspublication. The information given is designed only as a guidance for safe handling, and is not to be considered a warranty orquality specification. The information relates only to the specific material designated and may not be valid for such material used incombination with any other materials or in any process, unless specified in the text.GHS Classification is acoording to JIS Z7252(2010). *JIS: Japanese Industrial Standards
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End of Safety Data Sheet
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