# Peptides

Table 1S. Results of mass spectrometry analyses

1st Match Reference

1

2

3

4

5

6

7

8

910

11

12

13

15

14

161718

93 kDa

73 kDa

70 kDa

58 kDa

56 kDa

52 kDa

50 kDa

45 kDa

43 kDa

40 kDa

32 kDa

30 kDa

29.5 kDa

110 kDa

42 kDa

19

Gelsolin Nodes et al. 1987

pyruvate kinase M2

calreticulin

heat shock protein90

Autocrine motility factor(AMF, neuroleukin, GIP) Fibronectin 1 isoform 3

Enolase 1

cytoskeletal actin

-

Annexin I (lipocortin I)

unclear NA

14-3-3 sigma (stratifin)

14-3-3 zeta

NA

NA

Hsp-27

25 kDa18 kDa16 kDa

unclear NA

NA

NA

35 kDa

**

*

*

*

*

unclear

unclear

*

*

Eustace and Jay, 2004

Fibronectin isoform 6 HSP70 Fragment

Score/MP

116/4

629/21

423/14

235/10

180/9

247/6

724/35

278/12

265/11

435/34

375/25

101/3

271/15; 214/17

* Oremek et al, 1999

Rokeach et al, 1991

Akoun et al. 1985

-

-

Gurney et al, 1986

Rothhut et al. 1983

Katz et al. 1999

unclear

unclear

-

Katz et al. 1999

Martin-Ventura et al. 1999

*Previously reported as a secreted molecules; “unclear”, not high enough score todraw a conclusion; NA, not applicable; The list of references is given at the end of Materials and Methods

References listed in Table 1S

Akoun, G.M. et al. (1985. Serum neurone-specific enolase, A marker for disease extent and response to therapy for small-cell lung cancer. Chest. 87, 39-43Eustace, B. K., and Jay, D.G. (2004) Extracellular roles for the molecular chaperone, hsp90. Cell Cycle, 3, 1098-1100

Gurney, M. E., et al. (1986) Neuroleukin: a lymphokine product of lectin-stimulated T cells. Science. 234, 574-581

Katz, A. B., and Taichman, L. B. (1999) A partial catalog of proteins secreted by epidermal keratinocytes in culture. J. Invest. Dermatol.112, 818-821

Martin-Ventura, J. L., et al. (2004) Identification by a differential proteomic approach of heat shock protein 27 as a potential marker of atherosclerosis. Circulation. 110, 2216-2219

Nodes, B. R., Shackelford, J. E., Lebherz, H. G. (1987) Synthesis and secretion of serum gelsolin by smooth muscle tissue. J. Biol. Chem. 262, 5422-5427

Oremek, G. M., Teigelkamp, S., Kramer, W., Eigenbrodt, E., and Usadel, K.H. (1999) The pyruvate kinase isoenzyme tumor M2 (Tu M2-PK) as a tumor marker for renal carcinoma. Anticancer Res. 19(4A), 2599-2601

Rokeach, L. A., et al. (1991) Characterization of the autoantigen calreticulin. J Immunol. 147, 3031-3039

Rothhut, B., Russo-Marie, F., Wood, J., DiRosa, M., and Flower, R. J. (1983) Further characterization of the glucocorticoid-induced antiphospholipase protein "renocortin". Biochem Biophys Res Commun.117, 878-884