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B A L A I P E N E L I T I A N B I O T E K N O L O G I P E R K E B U N A N . IN D O N E S I A . T.W. Darmono 1) , A. Purwantara 1) , H. Widiastuti 1) , G. A. Rahim 2) , D. Asmono 2) , Mustofa 2) 1) Indonesian Biotechnology Research Institute for Estate Crops - Bogor 2) PT Sampoerna Agro - Palembang Keywords: Subsoil, EFB, nursery, AMF, Ganoderma, Trichoderma.

Penggunaan subsoil sebagai media pembibitan kelapa sawit (Presentasi TW Darmono et al di IOPC 2010, Yogyakarta: Juni 1-3, 2010)

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Ketersediaan topsoil sebagai media pembibitan kelapa sawit semakin terbatas sehingga harus diambil dari tempat yang jauh yang memerlukan biaya transport yang mahal dan belum tentu bebas dari Ganoderma. Untuk mengatasi kesulitan tersebut, pekerja yang tidak memiliki pengetahuan yang cukup bisa melakukan tindakan mengambil tanah di perkebunan kelapa sawit yang terinfestasi oleh Ganoderma. Hal itu tentu akan sangat membahayakan karena dapat menularkan Ganoderma melalui medium pertumbuhan bibit. Untuk mengatasi permasalahan tersebut, teknologi pembibitan dengan menggunakan subsoil telah dikembangkan seperti yang dilaporkan pada presentasi terlampir. Subsoil harus dicampur dengan kompos TKKS yang dibuat dengan menggunakan biodekomposer Promi. Biodekomposer Promi mengandung Trichoderma harzianum DT 38 dan T. pseudikoningii DT 39 yang teruji mampu mematikan Ganoderma. Semoga bermanfaat bagi masyarakat perkelapasawitan. DT.

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Page 1: Penggunaan subsoil sebagai media pembibitan kelapa sawit (Presentasi TW Darmono et al di IOPC 2010, Yogyakarta: Juni 1-3, 2010)

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T.W. Darmono 1), A. Purwantara1), H. Widiastuti1),

G. A. Rahim2), D. Asmono2), Mustofa2)

1)Indonesian Biotechnology Research Institute for Estate Crops - Bogor2) PT Sampoerna Agro - Palembang

Keywords: Subsoil, EFB, nursery,

AMF, Ganoderma, Trichoderma.

Page 2: Penggunaan subsoil sebagai media pembibitan kelapa sawit (Presentasi TW Darmono et al di IOPC 2010, Yogyakarta: Juni 1-3, 2010)

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Demand of oil palm seedlings remains Demand of oil palm seedlings remains

high (at least until today).high (at least until today).

Until recently, media for both preUntil recently, media for both pre-- and and

mainmain--nurseries are composed of nurseries are composed of

mainly top soils.mainly top soils.

Top soils nearby plantation nurseries is Top soils nearby plantation nurseries is

getting limited. Consequently, it needs getting limited. Consequently, it needs

to be collected from a distance to be collected from a distance

location (expensive transportation)location (expensive transportation)

Page 3: Penggunaan subsoil sebagai media pembibitan kelapa sawit (Presentasi TW Darmono et al di IOPC 2010, Yogyakarta: Juni 1-3, 2010)

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Danger of using near by soils in the Danger of using near by soils in the

plantation infested with plantation infested with GanodermaGanodermaby untrained workers.by untrained workers.

potent disease transmission potent disease transmission

through soil mediumthrough soil medium

X

Page 4: Penggunaan subsoil sebagai media pembibitan kelapa sawit (Presentasi TW Darmono et al di IOPC 2010, Yogyakarta: Juni 1-3, 2010)

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Provide cheap and healthy Provide cheap and healthy

medium in oil palm seedling medium in oil palm seedling

preparationpreparation

Free of Free of GanodermaGanoderma inoculuminoculum

Contain selected Contain selected TrichodermaTrichoderma

harzianumharzianum DT 38 and DT 38 and T. T.

pseudoconingiipseudoconingii DT39 DT39

antagonistic to antagonistic to GanodermaGanoderma

Contain selected VAM Contain selected VAM

Less inorganic fertilizer useLess inorganic fertilizer use

Page 5: Penggunaan subsoil sebagai media pembibitan kelapa sawit (Presentasi TW Darmono et al di IOPC 2010, Yogyakarta: Juni 1-3, 2010)

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Make compost from organic materials Make compost from organic materials

available in the plantation (EFB and Fruit available in the plantation (EFB and Fruit

Fiber) using decomposer containing Fiber) using decomposer containing T. T.

harzianumharzianum DT38 and DT38 and T. T. pseudokingiipseudokingii DT DT

39 formulated as 39 formulated as PromiPromiR

Page 6: Penggunaan subsoil sebagai media pembibitan kelapa sawit (Presentasi TW Darmono et al di IOPC 2010, Yogyakarta: Juni 1-3, 2010)

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Sandwiched “wellSandwiched “well--composted” EFB composted” EFB

containing containing biocontrolbiocontrol agents (1/3 agents (1/3

volume) in between subsoil layers volume) in between subsoil layers

in polybag.in polybag.

Place 50 g of Place 50 g of MycorrhizaeMycorrhizae

in the previously made in the previously made

plant hole in each MN plant hole in each MN

polybag before planting.polybag before planting.

Page 7: Penggunaan subsoil sebagai media pembibitan kelapa sawit (Presentasi TW Darmono et al di IOPC 2010, Yogyakarta: Juni 1-3, 2010)

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Determine the suitability of sub soil Determine the suitability of sub soil

as medium for oil palm nurseries as medium for oil palm nurseries

after being supplemented with EFB after being supplemented with EFB

compost (prepared as above) and compost (prepared as above) and

enriched with enriched with ArbuscularArbuscular

MycorrhizalMycorrhizal Fungus (AMF)Fungus (AMF)

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Materials :Materials :Materials :Materials :

Seedlings of PN (prepared using subsoil medium supplemented with 200 g EFB

compost enriched with 20 g AMF).

Well-Composted EFB containing biocontrol agents Trichoderma harzianum DT38 and

T. pseudokongii DT 39.

Biofertilizer MizaPus

Methodology :Methodology :Methodology :Methodology :

Completely randomized design 3 x 3 x 15.000

Treatments :

K (100% inorganic fertilizer

P1 (50% inorganic fertilizer + 8,5 kg EFB compost)

P2 (50% inorganic fertilizer + 8,5 kg EFB compost) + 50 g Biofertilizer MizaPlus

Page 9: Penggunaan subsoil sebagai media pembibitan kelapa sawit (Presentasi TW Darmono et al di IOPC 2010, Yogyakarta: Juni 1-3, 2010)

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Seedling height, number of leaf, and stem diameter of oil palm at

the end of the MN stage in response to treatment (Data are average

of three replications of 10 plants each).

1

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Seedling fresh weight (left) and dry weight (right) at the end of

MN stage in response to treatment. (Data are average of three

replications and each replication 10 plants).

2

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Content of N, P and K of root and N, P and K uptake of oil palm

root at the end of MN stage in response to treatment. (Data are

average of three replications of 10 plants each).

3

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P content of leaf P uptake of leaf

P content and P uptake of oil palm seedling at the end of MN stage in

response to treatment. (Data are average of three replications of 10

plants each).

4

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Soil chemical properties of oil palm seedlings at the end of MN

stage in response to treatment. (Data are average of three

replications of 10 plants each

5

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Addition of wellAddition of well--composted EFB in sub soil medium either composted EFB in sub soil medium either

with or without AMF resulted in better growth performance of with or without AMF resulted in better growth performance of

the seedlings at MN stage than that of subsoil supplemented the seedlings at MN stage than that of subsoil supplemented

with 100% inorganic fertilizer.with 100% inorganic fertilizer.

Nutrient status in all treatments seemed to be insignificantly Nutrient status in all treatments seemed to be insignificantly

different, although there is a tendency that both in root and different, although there is a tendency that both in root and

leaf, the uptake of nutrients was higher in medium treated leaf, the uptake of nutrients was higher in medium treated

with compost alone or compost with AMF.with compost alone or compost with AMF.

50% reduction of inorganic fertilizer in the medium could be 50% reduction of inorganic fertilizer in the medium could be

conducted if subsoil medium is supplemented with EFB conducted if subsoil medium is supplemented with EFB

compost and enriched with AMF).compost and enriched with AMF).

Page 15: Penggunaan subsoil sebagai media pembibitan kelapa sawit (Presentasi TW Darmono et al di IOPC 2010, Yogyakarta: Juni 1-3, 2010)

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Subsoil medium supplemented with EFB compost containing Subsoil medium supplemented with EFB compost containing

biocontrolbiocontrol agents and enriched with AMF possesses better agents and enriched with AMF possesses better

features than that of subsoil supplemented with inorganic features than that of subsoil supplemented with inorganic

fertilizer due the following reasons:fertilizer due the following reasons:

a). Use less inorganic chemicala). Use less inorganic chemical

b). Contain selected biological control agents suppressive and b). Contain selected biological control agents suppressive and

antagonistic to antagonistic to GanodermaGanoderma..

c). Free of c). Free of GanodermaGanoderma infestation.infestation.

d). Less expensive than that of if top soil were used.d). Less expensive than that of if top soil were used.