AGENC( FOR INTERNA7IOIAL DEVELOPMENT WASHINGTON DC 20523

DATE

MEMCRAINDUM

TO ATDPPCCDIEDI room 209 SA-18

FROM Al)Cr Victoria Or e

SUBJECT Trainrnitt~a of AIDSCI Progress Report(s)

Attache-d for periinert retent ionproper disposition is the following

AIDSCI Progre Report No

Attachment

P R O G R E S S REPORT NO 4

NATURAL INSECTICIDES FOR SPODOPTERA LITIJRA FABR

A RESEARCH PROJLCT

USA IDPSTC P4 OGRAM

GRANT NO 936-5512-gtO0-5032-00

SUBMITTED) BY

PICHE WIRIYACHITRA

PRINCE OF SONGKIA UNIVERS]

HAT YAI THAILAND

ROM r~irl

PROJECT PROFILE

Country Thailand

Grant No 936-5542-G-00-5032-00

Science and Technology CooperationProgram Program on

Project Title Natural Insecticides for Spooptera litura Fabr

Projct Leader Pichaet Wiriyachitra

Organization Prince of Songkla University Hat Yai

Co-invcstiqators Prachaval Sukumalanand

Total Froject Budget US $ 150000

Froject Dration 3 years

Poportinc Pirind I December 1986 to 31 May 1988

for This Period 133021179 BahtDudget Allocation

P R O G R E S S REPORT NO 4

NATURAL INSECTICIDES FOR SPODOPTERA LITIJRA FABR

A RESEARCH PROJLCT

USA IDPSTC P4 OGRAM

GRANT NO 936-5512-gtO0-5032-00

SUBMITTED) BY

PICHE WIRIYACHITRA

PRINCE OF SONGKIA UNIVERS]

HAT YAI THAILAND

ROM r~irl

PROJECT PROFILE

Country Thailand

Grant No 936-5542-G-00-5032-00

Science and Technology CooperationProgram Program on

Project Title Natural Insecticides for Spooptera litura Fabr

Projct Leader Pichaet Wiriyachitra

Organization Prince of Songkla University Hat Yai

Co-invcstiqators Prachaval Sukumalanand

Total Froject Budget US $ 150000

Froject Dration 3 years

Poportinc Pirind I December 1986 to 31 May 1988

for This Period 133021179 BahtDudget Allocation

PROJECT PROFILE

Country Thailand

Grant No 936-5542-G-00-5032-00

Science and Technology CooperationProgram Program on

Project Title Natural Insecticides for Spooptera litura Fabr

Projct Leader Pichaet Wiriyachitra

Organization Prince of Songkla University Hat Yai

Co-invcstiqators Prachaval Sukumalanand

Total Froject Budget US $ 150000

Froject Dration 3 years

Poportinc Pirind I December 1986 to 31 May 1988

for This Period 133021179 BahtDudget Allocation

BACKGROUND

S itulta Pabr is a widespread insect pest It is polyphagous

and regarded as one of the most damaging pests in the world agriculture

Pandy and Srivastava (1967) reported that the insect attacks 112 species

of cultivated crops all over the world In Thailand at least 30

var-leties of plants are found to 1-e host plants of this insect

These include v-getables floral plants ornamental plants economic

field crops and weeds

The time required for egg larval and pupal development are

2-3 14 and 7-8 days respectively2 The adult longevity is 8-10 days

in the male and 10-12 days in the female The larva molts 5 times

It is at this stage that the insect is most damaging Since the early

larval instars are quite vunerable to external damage it has been

chcsen 3isthe target organism for this work

For self-defence it is not surprising that plants would contain

substances which are inst-ct antifeedants insect repellents and insectishy

cides Some naturally occurring antifeedants which have been published

3 4are glycosides of steroidal alkaloids demissine solacauline

5 6 tomatine leptines I and 11 ring A enone andor ring D aromatic

steroids the nicandrenones and other 7890 juglone (5-hydroxynapthoshy

1o 11 12quinone) the isoquinoline alkaloid isoboldine phenylpropanoids

germrcrane sesquiterpenes shiromodiol and shiromol 1314 efit-clerodane

16ndc 15and clerodane diterpenes clerodendrin caryoptin and others

mel iantriol 7 azadirachtin 18 xylomolin 9the hydroxylated steroe(i

ins 0 harrisonin21 polyqodii ugandensidial and warburganal 22

l 23 i n4 25~irfej 2rnd 24i rindid)id 2 and unedoside However

there is no report of any of these fr+ ostances being used effectively

in agriculture

- 2 -

A number of naturally occurring compounds have been found to

exhibit insecticidal activity These include alkaloids terpenoids

phenolics cinines chromenes prenylated guinones and sesqiiterpenes

esterified with phenolic acids 2 6 Commercially the substances which

have found their way to agricultuiral application are nicotine derris

27 pyrethrum and the newer product ryania Crude extracts of the

plant producing these r ubstances or dried and powdered plant tissue

have a long history of use i SE Asia as ii secticides However none

of there natural products is a universal insecticide Tobacco leaf

containing nicotine is subject to attack by several insects the root

of derris-containing plant is attacked by the larvae of a species of

Neetle the flower from which pyrethrins are extracted needs synthetic

invecticidros for protection f-om insects in the fields In addition

inrects can develop resistance to a certain chemicals after a long

exposure New and better insecticides will therefore have to be

constantly souriht

Since S UtU4( is one of the major pests in SE Asia the

6ircovery of a new and more effective insecticide with low mammalian

toxicity from local plants woul6 produce enormous benefit to the region

ar well as to ThMiand in general

ORJECTI VES

1 To isolate and identify natural insecticides and antifecdants

which have ow mammalian toxicity for S 4W1a

2 Extract or Fynthesizing those compounds on a practical

scale and design of industrial extraction procedure

3 Evaluation of their applicability in the field

MATERIALS AND METHODS

Plants investigated were collected in the southern region of

ThiIand S~imples were clascified taxonomically and vouchers were

deposited in tho herbariu in the DeparuiTit of Biology Prince of

Songk la Uni verit y

Ground plant materials were steam distilled or exhaustively

extracted with solvents The extractssteam ditiliates were screened

for inmsecticidal and antifeedant activity with S titula and the active

extracts reiolved into fractions Each fraction was screened for

activity aInd the active fraczin again separated into individual

constituerit uinq quick column and preparative layer chromatography

cnn silica geI

1 ich o the individual components was tested again for activity

and the LC value of thV active compound determined The structure of

the compound was elucidated using spectroscopic techniques

-4-

Ground

Plant Material

extracted with alcohol

-ive screncu for Crude Extract screened forgt -ive

lot investigated activity activity not investigated

screened for

activity

Activ2 Extrac

f ractiona tion

II Fraction IIIIraction~Xdctio

screened for activity

-ive -iye not investigated not investigated

LActive Fractiorl

iCompoundI rCompound II Coipound II

screened for activity

structure elucidated

-i____-N__ lActive compoundl

-ive structure elucidated

LC50 structure determined elucidated

syntheses comparison with degradation kno-wn a-ces

Diagram showing work plan

- 5 -

The leaf dipping (LD) method was used for the detection of

stomach poisor1ing and anti feeding activity the third instars of

Spodopvta etuaza 1lAvae were left to feed on the leaves of V-gna

Aadiata which hil ten immersed in the tested solution then airshy

dr i-d Ten larvaewre used in each of the four replications

(one as control ) The numbe-r of dead organisms was recorded at

the end of e-ch day The amount of the leaf consumption and the

larvae sine wi-re also estimatied

The topicFil application (TA) method was used for the detection

of dermal toxicity the tested rolution was dropped on the thorax

of the thi rd in5tar Iarvae The organisms were then left to feed

on untreateo leaveni of Vigna Aadiata in a plastic Tontainer Ten

larvae were und in each of the four replications (one as control)

The ncii-w-r of ci d organisms was recorded at the end of each day

Le1D coipl ed with TA method ws used for the detection of

the comlir cd effect of stomach poiconing and dermal toxicity The

tested 1ol tiin Wan drooped on trie thorax of the third instar larvae

The orqani w-wr( then left to feed on the leaves of Vigna adinta

which har] 1en imcm reId in the same tented solution then air-dried

Ten ]arvia were uicu in each o the four replications (one as control)

Thn numbr- of dead organisms was recorded at the end of each day

- 2 -

A number of naturally occurring compounds have been found to

exhibit insecticidal activity These include alkaloids terpenoids

phenolics cinines chromenes prenylated guinones and sesqiiterpenes

esterified with phenolic acids 2 6 Commercially the substances which

have found their way to agricultuiral application are nicotine derris

27 pyrethrum and the newer product ryania Crude extracts of the

plant producing these r ubstances or dried and powdered plant tissue

have a long history of use i SE Asia as ii secticides However none

of there natural products is a universal insecticide Tobacco leaf

containing nicotine is subject to attack by several insects the root

of derris-containing plant is attacked by the larvae of a species of

Neetle the flower from which pyrethrins are extracted needs synthetic

invecticidros for protection f-om insects in the fields In addition

inrects can develop resistance to a certain chemicals after a long

exposure New and better insecticides will therefore have to be

constantly souriht

Since S UtU4( is one of the major pests in SE Asia the

6ircovery of a new and more effective insecticide with low mammalian

toxicity from local plants woul6 produce enormous benefit to the region

ar well as to ThMiand in general

ORJECTI VES

1 To isolate and identify natural insecticides and antifecdants

which have ow mammalian toxicity for S 4W1a

2 Extract or Fynthesizing those compounds on a practical

scale and design of industrial extraction procedure

3 Evaluation of their applicability in the field

MATERIALS AND METHODS

Plants investigated were collected in the southern region of

ThiIand S~imples were clascified taxonomically and vouchers were

deposited in tho herbariu in the DeparuiTit of Biology Prince of

Songk la Uni verit y

Ground plant materials were steam distilled or exhaustively

extracted with solvents The extractssteam ditiliates were screened

for inmsecticidal and antifeedant activity with S titula and the active

extracts reiolved into fractions Each fraction was screened for

activity aInd the active fraczin again separated into individual

constituerit uinq quick column and preparative layer chromatography

cnn silica geI

1 ich o the individual components was tested again for activity

and the LC value of thV active compound determined The structure of

the compound was elucidated using spectroscopic techniques

-4-

Ground

Plant Material

extracted with alcohol

-ive screncu for Crude Extract screened forgt -ive

lot investigated activity activity not investigated

screened for

activity

Activ2 Extrac

f ractiona tion

II Fraction IIIIraction~Xdctio

screened for activity

-ive -iye not investigated not investigated

LActive Fractiorl

iCompoundI rCompound II Coipound II

screened for activity

structure elucidated

-i____-N__ lActive compoundl

-ive structure elucidated

LC50 structure determined elucidated

syntheses comparison with degradation kno-wn a-ces

Diagram showing work plan

- 5 -

The leaf dipping (LD) method was used for the detection of

stomach poisor1ing and anti feeding activity the third instars of

Spodopvta etuaza 1lAvae were left to feed on the leaves of V-gna

Aadiata which hil ten immersed in the tested solution then airshy

dr i-d Ten larvaewre used in each of the four replications

(one as control ) The numbe-r of dead organisms was recorded at

the end of e-ch day The amount of the leaf consumption and the

larvae sine wi-re also estimatied

The topicFil application (TA) method was used for the detection

of dermal toxicity the tested rolution was dropped on the thorax

of the thi rd in5tar Iarvae The organisms were then left to feed

on untreateo leaveni of Vigna Aadiata in a plastic Tontainer Ten

larvae were und in each of the four replications (one as control)

The ncii-w-r of ci d organisms was recorded at the end of each day

Le1D coipl ed with TA method ws used for the detection of

the comlir cd effect of stomach poiconing and dermal toxicity The

tested 1ol tiin Wan drooped on trie thorax of the third instar larvae

The orqani w-wr( then left to feed on the leaves of Vigna adinta

which har] 1en imcm reId in the same tented solution then air-dried

Ten ]arvia were uicu in each o the four replications (one as control)

Thn numbr- of dead organisms was recorded at the end of each day

MATERIALS AND METHODS

Plants investigated were collected in the southern region of

ThiIand S~imples were clascified taxonomically and vouchers were

deposited in tho herbariu in the DeparuiTit of Biology Prince of

Songk la Uni verit y

Ground plant materials were steam distilled or exhaustively

extracted with solvents The extractssteam ditiliates were screened

for inmsecticidal and antifeedant activity with S titula and the active

extracts reiolved into fractions Each fraction was screened for

activity aInd the active fraczin again separated into individual

constituerit uinq quick column and preparative layer chromatography

cnn silica geI

1 ich o the individual components was tested again for activity

and the LC value of thV active compound determined The structure of

the compound was elucidated using spectroscopic techniques

-4-

Ground

Plant Material

extracted with alcohol

-ive screncu for Crude Extract screened forgt -ive

lot investigated activity activity not investigated

screened for

activity

Activ2 Extrac

f ractiona tion

II Fraction IIIIraction~Xdctio

screened for activity

-ive -iye not investigated not investigated

LActive Fractiorl

iCompoundI rCompound II Coipound II

screened for activity

structure elucidated

-i____-N__ lActive compoundl

-ive structure elucidated

LC50 structure determined elucidated

syntheses comparison with degradation kno-wn a-ces

Diagram showing work plan

- 5 -

The leaf dipping (LD) method was used for the detection of

stomach poisor1ing and anti feeding activity the third instars of

Spodopvta etuaza 1lAvae were left to feed on the leaves of V-gna

Aadiata which hil ten immersed in the tested solution then airshy

dr i-d Ten larvaewre used in each of the four replications

(one as control ) The numbe-r of dead organisms was recorded at

the end of e-ch day The amount of the leaf consumption and the

larvae sine wi-re also estimatied

The topicFil application (TA) method was used for the detection

of dermal toxicity the tested rolution was dropped on the thorax

of the thi rd in5tar Iarvae The organisms were then left to feed

on untreateo leaveni of Vigna Aadiata in a plastic Tontainer Ten

larvae were und in each of the four replications (one as control)

The ncii-w-r of ci d organisms was recorded at the end of each day

Le1D coipl ed with TA method ws used for the detection of

the comlir cd effect of stomach poiconing and dermal toxicity The

tested 1ol tiin Wan drooped on trie thorax of the third instar larvae

The orqani w-wr( then left to feed on the leaves of Vigna adinta

which har] 1en imcm reId in the same tented solution then air-dried

Ten ]arvia were uicu in each o the four replications (one as control)

Thn numbr- of dead organisms was recorded at the end of each day

-4-

Ground

Plant Material

extracted with alcohol

-ive screncu for Crude Extract screened forgt -ive

lot investigated activity activity not investigated

screened for

activity

Activ2 Extrac

f ractiona tion

II Fraction IIIIraction~Xdctio

screened for activity

-ive -iye not investigated not investigated

LActive Fractiorl

iCompoundI rCompound II Coipound II

screened for activity

structure elucidated

-i____-N__ lActive compoundl

-ive structure elucidated

LC50 structure determined elucidated

syntheses comparison with degradation kno-wn a-ces

Diagram showing work plan

- 5 -

The leaf dipping (LD) method was used for the detection of

stomach poisor1ing and anti feeding activity the third instars of

Spodopvta etuaza 1lAvae were left to feed on the leaves of V-gna

Aadiata which hil ten immersed in the tested solution then airshy

dr i-d Ten larvaewre used in each of the four replications

(one as control ) The numbe-r of dead organisms was recorded at

the end of e-ch day The amount of the leaf consumption and the

larvae sine wi-re also estimatied

The topicFil application (TA) method was used for the detection

of dermal toxicity the tested rolution was dropped on the thorax

of the thi rd in5tar Iarvae The organisms were then left to feed

on untreateo leaveni of Vigna Aadiata in a plastic Tontainer Ten

larvae were und in each of the four replications (one as control)

The ncii-w-r of ci d organisms was recorded at the end of each day

Le1D coipl ed with TA method ws used for the detection of

the comlir cd effect of stomach poiconing and dermal toxicity The

tested 1ol tiin Wan drooped on trie thorax of the third instar larvae

The orqani w-wr( then left to feed on the leaves of Vigna adinta

which har] 1en imcm reId in the same tented solution then air-dried

Ten ]arvia were uicu in each o the four replications (one as control)

Thn numbr- of dead organisms was recorded at the end of each day

- 5 -

The leaf dipping (LD) method was used for the detection of

stomach poisor1ing and anti feeding activity the third instars of

Spodopvta etuaza 1lAvae were left to feed on the leaves of V-gna

Aadiata which hil ten immersed in the tested solution then airshy

dr i-d Ten larvaewre used in each of the four replications

(one as control ) The numbe-r of dead organisms was recorded at

the end of e-ch day The amount of the leaf consumption and the

larvae sine wi-re also estimatied

The topicFil application (TA) method was used for the detection

of dermal toxicity the tested rolution was dropped on the thorax

of the thi rd in5tar Iarvae The organisms were then left to feed

on untreateo leaveni of Vigna Aadiata in a plastic Tontainer Ten

larvae were und in each of the four replications (one as control)

The ncii-w-r of ci d organisms was recorded at the end of each day

Le1D coipl ed with TA method ws used for the detection of

the comlir cd effect of stomach poiconing and dermal toxicity The

tested 1ol tiin Wan drooped on trie thorax of the third instar larvae

The orqani w-wr( then left to feed on the leaves of Vigna adinta

which har] 1en imcm reId in the same tented solution then air-dried

Ten ]arvia were uicu in each o the four replications (one as control)

Thn numbr- of dead organisms was recorded at the end of each day

-6shy

RESULTSDISCUSSIONTABLES

Reinvestigation of the antifeeding activity of 4 Agaia

species eg A adokata A adarntica A a49entea A quamuto6a

revealed the significant acivities even at low concentration Attempt

was then rcade to fully explore the reaction of S L-tuta larvae to these

extractsfractions

A soy bean antificial diet was admixed with these crude

extractsfractions and used as feed for the larvae Outstanding

results were obtained frcm -xtractsfractions from A odottata

The feeding of larvae was significantly reduced the larval growth was

therefore slow They eventually died Table I 11 1 show these

resultf In detail

Ill 1

TABLE

MCRTALITY OF 5 LITUP F FED ON ARTIFICIAL DIET MIXED WITH EXTRAZT OF A SPECILS

ACCUNULATIVL MDRTAL1TY (ABBOTTS FORMULA)

PLANT SPECIES FPACTIOH CONC(PPM)

DAY 1 3 4 5 7 10

Aglaia d C H 10000 714 714 7 14 714 714 714 714 714 714 -

CH Cl 10000 667 1333 1667 10000 - - - - -

6000 337 6b7 3333 9667 10000 - -

6000 333 667 33 33 9667 10300 - -

4000 000 000 5333 10000 -- - -

2000 000 000 3333 7000 9333 10000 - -

1) QC(CH| 6000 000 000 000 000 0 00 000 000 000

2) QC(CHIICl) 10000 000 000 344 5714 92 l 9b43 10000 -

8000 000 000 1034 4285 7t57 10000 - -

6000 000 000 344 2459 7857 9643 10000 - -

4000 1000 1000 1723 2499 3929 5714 6785 8571 10000 -

2000 000 000 000 000 1425 3214 4285 75 uO 9260 9615

iABLi 1

MORTALITY OF LQ F FED ON ARTIFICIAL DIET MIXED WITH EXTRACT UF 5 5FECIi

ACCuMULATIVE M RTALITY AbTT5 FORMULA) PLANT SPECIES FRACTON CCNC(Pk-H _

DAY 1 3 4 5 6 7 a 9 20

Aalaia cQrora 3 QCtC H C CI 31) 10000 000 000 000 OOu CO0 000 000 000 000 000

8000 000 000 000 000 000 OOu 00a 000 0O O

6000 334 334 334 334 334 334 334 334 334 334

4000 000 000 OOu 000 000 000 C00 000 000 000

2000 000 0Ou 000 000 010 000 000 000 000 000

41 QC(CHClI I0000 000 000 10000 - - - - - - -

8000 000 000 10000 - - - -

600C 000 333 9000 10000 - - -

4000 000 000 1334 10000 - - -

2000 000 000 3000 9311 10000 --

S QC HampOH) 10000 334 344 551 10000 - - -

8000 000 000 3000 9311 10000 - -

6000 000 000 6238 9310 10000 - -

2000 000 000 2758 8965 9643 10000 -

MORTALITY OF URA F FED ON ARTIFICIAL DIET MIXED WITH EATRACT Ok 6 SPECIES

ACCUMULATIVE I HCRTALITY (A-OTT- FORMULA)

PLANT SPECIESI FRACTICH CONC(PPM)

DAY 1 2 3 4 5 b 7 9 10 11

A______ oOH 10000 000 b67 2333 9333 10000 -

50-0 000 333 E66 7

66 67 89B5 9655 10(00

6000 00 000 6000 6b67 6620 9310 9b55 l00 00 - -

4000 000 000 333 333 1667 4462 6207 8276 8276 9655 0000

2000 000 000 333 333 344 689 2413 2658 3792 5555 -

EtOAc (insol) 10000 000 000 000 000 000 000 344 2758 3447 5172 -

000 000 000 000 030 000 OOG 344 20 EL 20E3 2758 -

6000 000 J00 000 000 000 000 3 44 344 10J4 IJ4 -

4000 000 0 00 000 0 00 000 00 0 00 0 0 000 000 -

2000 000 000 000 000 000 000 OCG 000 000 000 -

tOAc (601) 10000 000 034 4E27 a6 55 100 00 - - - - -

6000 000 689 5517 C000 - - -

6000 000 687 2758 965 10000 - -

4000 000 000 1034 9655 10000 - - -

2000 000 689 2069 6207 9365 10000 - - -

MORTALITY OF F FD ON AKTIFICIAL -4rLXFD WITH LtTPACT CF __ _pCI_

A ACCUNfULATIvE HCRTALI7Y (ABc-S pOprW LA)

Day 1 3 4 5 6 7 9 10 11 12 13

tCCl 30

600

400

200

000

000

000

000

000

000

000

000

000

500

000

000

500

500

000

000

3500

I000

000

000

6500

1100

500

000

9000

20c0

000

000

9500

3000

3000

500

10000

3530

4000

500

-

6000

4300

1500

-

6000

4000

1500

6500

4000

1500

7500

4000

1500

-HGOA Soo

So0

40

200

000

000

000

000

000

500

000

000

000

500

500

000

000

500

500

000

500

500

500

000

500

500

Scd

0clo

500

500

500

000

500

soo

500

000

500

500

500

000

500

500

500

000

500

500

500

000

10500

500

500

500

000

1500

500

500

o00

000

13 14 15 1 b 17 18 19 20 21 22 23 _4 25 26 27 28 9 30

3

3

3

75 00

40 00

1500

7500

-000

1000

8421

7894

1578

8947

8421

2631

9847

8947

3157

8947

9473

3157

8947

S473

8157

9473

9473

7b84

10000 -

9473 9473

421 4210

--

10000

5263 5263

-

5263 5263

-

- - -

3 500

5 00

C(00

1500

500

50000

000

1500

1000

500

000

3000

1000

500

000

4000

1000

500

000

400

1000

500

000

4000

1000

500

000

4000

000

500

000

5000

10 10

500

000

5500

1000

500

500

5500

1000

500

500

6000

1000

500

500

6500

1000

500

500

7000

1000

500

500

7000

1000

500

500

8000

1000

500

500

BO00

2500

500

500

8500

3000

500

500

TABLE

MORTALITY OF 2- LIMamp F FED ON ARTIFICIAL DrT 4 Pr 15WITH EXT ACT3F

PLANT SPECIES FRACTION

I

CONC (PPM)

Day 1

ACCUHULA2IL

3 4

HORTALITY

5

iAbbOT

b

5 FOPHULA)

7 9

holaiA andaani1 L C M 10000

8000

000

000

000

000

000

000

333

333

3 33

356

3j3

3 56

3 33

356

333

356

33

356

333

356

6000 000 000 000 000 000 000 000 0 U0 000 000

lCl

4000

2000

10000

000

000

000

000

000

000

000

000

333

000

000

1000

000

nO0

1071

000

( Go

1071

000

000

o 71

000

000

071

000

000

i01

000

0 o0

10 71

0000

6000

000

000

000

0uO

000

000

1667

0 0

16 67

000

1667

000

16b7

000

1b67

000

1 7

000

1667

000

4000 0O r

000 000 000 000 000 000 0 00 300 000

2000 000 000 0 00 000 000 000 O00 000 000 000

1ON10000

8000

000

000

000

000

333

000

1000

000

1334

000

1334

000

2000

000

2334

333

2334

333

2334

333

6000

4000

000

000

000

000

000

000

000

000

000

300

0 00

000

000

000

333

00G

333

0

333

2000 000 000 000 000 000 000 0 00 000 000 000

TMALhE i

tKDR-ALITY 0 S-L F FED ON ARTFICIAL DIET MIXED WITH Z TCT Ot AGIpound PECIES

ACCUV2-LTIVE 1-7RTALITY BABOf- ORMULA

PLA14T SPECIE5 FRACTICH o (PPM)

Djy I3 4 7 3 10

ampalsajZ C H 10000 000 0 00 000 000 0UO C00 000 010 000 000

8000 333 333 333 333 333 333 333 333 333 333

6000 000 000 000 000 000 000 000 0C 000 333

4000 000 000 000 000 000 000 000 00 0 00 000

2000 000 000 000 000 000 000 000 000 000 000

CHCl 10000 000 000 000 2000 6333 7000 6333 9333 10000 -

2000 000 00-0 000 333 4333 6333 n3 33 9667 10000 -

6000 000 000 D00 1667 2333 4-07 6000 667 332 966

4000 000 000 333 1000 2667 4333 1239000 i66b 10000

2000 000 000 333 1333 20CO 2000 b 00u 7333 d3 3^ 933

MaOH 10000 000 000 333 1000 2333 5333 7000 8333 86 67 9333

8000 333 333 667 1667 3667 5000 6000 -000 3 33 9333

6000 000 000 000 667 1000 3000 4667 70 00 8333 9667

4000 000 000 000 000 333 1000 2333 3 67 60 3u 3 13

2000 000 000 333 333 333 666 2000 4000 4b6 766

TABE I

MORTALITY OF 5_LIrUF F FED ON ARTIFICIAL DIET MIXED WITH EXTRACT OF A SPECES

ACCUMULATIVE- MORTALITY (ABBO--5 FOFLULAI

PLANT SPECIES I FRACTION CONC (PPr)

Day 1 2 3 4 5 6 7 8 9 10

S___ CH 10000 000 000 000 000 000 000 000 6b 7

667 67

8000 000 000 000 000 Cooo 000 000 0 00 0 0o 0 00

6000 000 000 000 000 000 000 000 0 00 000 000

4000 000 000 000 000 000 000 000 000 000 000

2000 000 000 000 000 000 000 000 000 000 000

CHC1 10000 000 000 000 000 000 000 000 000 000 000

8000 000 000 000 000 000 000 000 000 CO0 000

60uo 000 000 000 000 000 00 00 0 01) 000c 0 00

4000 000 000 000 000 000 000 u00 000 000 010

20v0 000 000 000 000 000 000 000 000 000 000

10000 000 000 000 000 000 000 OQO 3O 00c Oc0

8000 000 000 000 000 000 000 000 OCO 00 000

6000 000 000 000 000 000 000 00A 000 000 00

4000 000 000 000 000 000 000 000 000 0 Ou 000

2000 000 000 000 000 000 000 000 000 000 003

TABLE I

MORTALITY OF I U F FE ON ARTIFICIAL DIET MIXED WITH ExTRFCT OF n SPECIES

CONC ACCUMULATIVE MORTALITY (ABBOTS FIRIULA)

PLANT 5PECI2- FRACTION (PPM)

Day 1 2 3 4 5 0 7 8 9 10

pygnxuIm RZ2=L 1rC H 10000 000 000 000 000 000 000 000 666 666 1667

8000 000 000 000 000 000 000 000 666 1000 2000

6000 000 000 OO 000 0 00 000 333 667 1000 2667

CHCl 10000 000 000 000 000 000 000 000 333 333 333

8000 ocO 000 000 000 000 000 000 333 333 333

6000 000 000 000 000 000 000 000 333 1667 2333

- cryit l 10000 000 000 000 000 00m 000 333 667 667 1667

8000 000 00L 000 000 000 333 33 b67 667 l6b7

6000 000 000 000 000 000 030 000 333 333 1000

21non-crystAl 10000 000 000 000 000 000 000 1000 1000 1667 1667

8000 000 000 000 000 000 000 000 000 000 000

K MoOH

6000

10000

000

OO

000

000

OjU

000

000

000

000

000

000

000

000

1333

333

1333

1000

1333

1333

1333

80L0 000 000 000 000 0 G0 000 000 000 000 000

6000 000 000 000 333 333 667 667 667 667 667

TABLE

MCRTALITY OF 5 LITUP F FED ON ARTIFICIAL DIET MIXED WITH EXTRAZT OF A SPECILS

ACCUNULATIVL MDRTAL1TY (ABBOTTS FORMULA)

PLANT SPECIES FPACTIOH CONC(PPM)

DAY 1 3 4 5 7 10

Aglaia d C H 10000 714 714 7 14 714 714 714 714 714 714 -

CH Cl 10000 667 1333 1667 10000 - - - - -

6000 337 6b7 3333 9667 10000 - -

6000 333 667 33 33 9667 10300 - -

4000 000 000 5333 10000 -- - -

2000 000 000 3333 7000 9333 10000 - -

1) QC(CH| 6000 000 000 000 000 0 00 000 000 000

2) QC(CHIICl) 10000 000 000 344 5714 92 l 9b43 10000 -

8000 000 000 1034 4285 7t57 10000 - -

6000 000 000 344 2459 7857 9643 10000 - -

4000 1000 1000 1723 2499 3929 5714 6785 8571 10000 -

2000 000 000 000 000 1425 3214 4285 75 uO 9260 9615

iABLi 1

MORTALITY OF LQ F FED ON ARTIFICIAL DIET MIXED WITH EXTRACT UF 5 5FECIi

ACCuMULATIVE M RTALITY AbTT5 FORMULA) PLANT SPECIES FRACTON CCNC(Pk-H _

DAY 1 3 4 5 6 7 a 9 20

Aalaia cQrora 3 QCtC H C CI 31) 10000 000 000 000 OOu CO0 000 000 000 000 000

8000 000 000 000 000 000 OOu 00a 000 0O O

6000 334 334 334 334 334 334 334 334 334 334

4000 000 000 OOu 000 000 000 C00 000 000 000

2000 000 0Ou 000 000 010 000 000 000 000 000

41 QC(CHClI I0000 000 000 10000 - - - - - - -

8000 000 000 10000 - - - -

600C 000 333 9000 10000 - - -

4000 000 000 1334 10000 - - -

2000 000 000 3000 9311 10000 --

S QC HampOH) 10000 334 344 551 10000 - - -

8000 000 000 3000 9311 10000 - -

6000 000 000 6238 9310 10000 - -

2000 000 000 2758 8965 9643 10000 -

MORTALITY OF URA F FED ON ARTIFICIAL DIET MIXED WITH EATRACT Ok 6 SPECIES

ACCUMULATIVE I HCRTALITY (A-OTT- FORMULA)

PLANT SPECIESI FRACTICH CONC(PPM)

DAY 1 2 3 4 5 b 7 9 10 11

A______ oOH 10000 000 b67 2333 9333 10000 -

50-0 000 333 E66 7

66 67 89B5 9655 10(00

6000 00 000 6000 6b67 6620 9310 9b55 l00 00 - -

4000 000 000 333 333 1667 4462 6207 8276 8276 9655 0000

2000 000 000 333 333 344 689 2413 2658 3792 5555 -

EtOAc (insol) 10000 000 000 000 000 000 000 344 2758 3447 5172 -

000 000 000 000 030 000 OOG 344 20 EL 20E3 2758 -

6000 000 J00 000 000 000 000 3 44 344 10J4 IJ4 -

4000 000 0 00 000 0 00 000 00 0 00 0 0 000 000 -

2000 000 000 000 000 000 000 OCG 000 000 000 -

tOAc (601) 10000 000 034 4E27 a6 55 100 00 - - - - -

6000 000 689 5517 C000 - - -

6000 000 687 2758 965 10000 - -

4000 000 000 1034 9655 10000 - - -

2000 000 689 2069 6207 9365 10000 - - -

MORTALITY OF F FD ON AKTIFICIAL -4rLXFD WITH LtTPACT CF __ _pCI_

A ACCUNfULATIvE HCRTALI7Y (ABc-S pOprW LA)

Day 1 3 4 5 6 7 9 10 11 12 13

tCCl 30

600

400

200

000

000

000

000

000

000

000

000

000

500

000

000

500

500

000

000

3500

I000

000

000

6500

1100

500

000

9000

20c0

000

000

9500

3000

3000

500

10000

3530

4000

500

-

6000

4300

1500

-

6000

4000

1500

6500

4000

1500

7500

4000

1500

-HGOA Soo

So0

40

200

000

000

000

000

000

500

000

000

000

500

500

000

000

500

500

000

500

500

500

000

500

500

Scd

0clo

500

500

500

000

500

soo

500

000

500

500

500

000

500

500

500

000

500

500

500

000

10500

500

500

500

000

1500

500

500

o00

000

13 14 15 1 b 17 18 19 20 21 22 23 _4 25 26 27 28 9 30

3

3

3

75 00

40 00

1500

7500

-000

1000

8421

7894

1578

8947

8421

2631

9847

8947

3157

8947

9473

3157

8947

S473

8157

9473

9473

7b84

10000 -

9473 9473

421 4210

--

10000

5263 5263

-

5263 5263

-

- - -

3 500

5 00

C(00

1500

500

50000

000

1500

1000

500

000

3000

1000

500

000

4000

1000

500

000

400

1000

500

000

4000

1000

500

000

4000

000

500

000

5000

10 10

500

000

5500

1000

500

500

5500

1000

500

500

6000

1000

500

500

6500

1000

500

500

7000

1000

500

500

7000

1000

500

500

8000

1000

500

500

BO00

2500

500

500

8500

3000

500

500

TABLE

MORTALITY OF 2- LIMamp F FED ON ARTIFICIAL DrT 4 Pr 15WITH EXT ACT3F

PLANT SPECIES FRACTION

I

CONC (PPM)

Day 1

ACCUHULA2IL

3 4

HORTALITY

5

iAbbOT

b

5 FOPHULA)

7 9

holaiA andaani1 L C M 10000

8000

000

000

000

000

000

000

333

333

3 33

356

3j3

3 56

3 33

356

333

356

33

356

333

356

6000 000 000 000 000 000 000 000 0 U0 000 000

lCl

4000

2000

10000

000

000

000

000

000

000

000

000

333

000

000

1000

000

nO0

1071

000

( Go

1071

000

000

o 71

000

000

071

000

000

i01

000

0 o0

10 71

0000

6000

000

000

000

0uO

000

000

1667

0 0

16 67

000

1667

000

16b7

000

1b67

000

1 7

000

1667

000

4000 0O r

000 000 000 000 000 000 0 00 300 000

2000 000 000 0 00 000 000 000 O00 000 000 000

1ON10000

8000

000

000

000

000

333

000

1000

000

1334

000

1334

000

2000

000

2334

333

2334

333

2334

333

6000

4000

000

000

000

000

000

000

000

000

000

300

0 00

000

000

000

333

00G

333

0

333

2000 000 000 000 000 000 000 0 00 000 000 000

TMALhE i

tKDR-ALITY 0 S-L F FED ON ARTFICIAL DIET MIXED WITH Z TCT Ot AGIpound PECIES

ACCUV2-LTIVE 1-7RTALITY BABOf- ORMULA

PLA14T SPECIE5 FRACTICH o (PPM)

Djy I3 4 7 3 10

ampalsajZ C H 10000 000 0 00 000 000 0UO C00 000 010 000 000

8000 333 333 333 333 333 333 333 333 333 333

6000 000 000 000 000 000 000 000 0C 000 333

4000 000 000 000 000 000 000 000 00 0 00 000

2000 000 000 000 000 000 000 000 000 000 000

CHCl 10000 000 000 000 2000 6333 7000 6333 9333 10000 -

2000 000 00-0 000 333 4333 6333 n3 33 9667 10000 -

6000 000 000 D00 1667 2333 4-07 6000 667 332 966

4000 000 000 333 1000 2667 4333 1239000 i66b 10000

2000 000 000 333 1333 20CO 2000 b 00u 7333 d3 3^ 933

MaOH 10000 000 000 333 1000 2333 5333 7000 8333 86 67 9333

8000 333 333 667 1667 3667 5000 6000 -000 3 33 9333

6000 000 000 000 667 1000 3000 4667 70 00 8333 9667

4000 000 000 000 000 333 1000 2333 3 67 60 3u 3 13

2000 000 000 333 333 333 666 2000 4000 4b6 766

TABE I

MORTALITY OF 5_LIrUF F FED ON ARTIFICIAL DIET MIXED WITH EXTRACT OF A SPECES

ACCUMULATIVE- MORTALITY (ABBO--5 FOFLULAI

PLANT SPECIES I FRACTION CONC (PPr)

Day 1 2 3 4 5 6 7 8 9 10

S___ CH 10000 000 000 000 000 000 000 000 6b 7

667 67

8000 000 000 000 000 Cooo 000 000 0 00 0 0o 0 00

6000 000 000 000 000 000 000 000 0 00 000 000

4000 000 000 000 000 000 000 000 000 000 000

2000 000 000 000 000 000 000 000 000 000 000

CHC1 10000 000 000 000 000 000 000 000 000 000 000

8000 000 000 000 000 000 000 000 000 CO0 000

60uo 000 000 000 000 000 00 00 0 01) 000c 0 00

4000 000 000 000 000 000 000 u00 000 000 010

20v0 000 000 000 000 000 000 000 000 000 000

10000 000 000 000 000 000 000 OQO 3O 00c Oc0

8000 000 000 000 000 000 000 000 OCO 00 000

6000 000 000 000 000 000 000 00A 000 000 00

4000 000 000 000 000 000 000 000 000 0 Ou 000

2000 000 000 000 000 000 000 000 000 000 003

TABLE I

MORTALITY OF I U F FE ON ARTIFICIAL DIET MIXED WITH ExTRFCT OF n SPECIES

CONC ACCUMULATIVE MORTALITY (ABBOTS FIRIULA)

PLANT 5PECI2- FRACTION (PPM)

Day 1 2 3 4 5 0 7 8 9 10

pygnxuIm RZ2=L 1rC H 10000 000 000 000 000 000 000 000 666 666 1667

8000 000 000 000 000 000 000 000 666 1000 2000

6000 000 000 OO 000 0 00 000 333 667 1000 2667

CHCl 10000 000 000 000 000 000 000 000 333 333 333

8000 ocO 000 000 000 000 000 000 333 333 333

6000 000 000 000 000 000 000 000 333 1667 2333

- cryit l 10000 000 000 000 000 00m 000 333 667 667 1667

8000 000 00L 000 000 000 333 33 b67 667 l6b7

6000 000 000 000 000 000 030 000 333 333 1000

21non-crystAl 10000 000 000 000 000 000 000 1000 1000 1667 1667

8000 000 000 000 000 000 000 000 000 000 000

K MoOH

6000

10000

000

OO

000

000

OjU

000

000

000

000

000

000

000

000

1333

333

1333

1000

1333

1333

1333

80L0 000 000 000 000 0 G0 000 000 000 000 000

6000 000 000 000 333 333 667 667 667 667 667

iABLi 1

MORTALITY OF LQ F FED ON ARTIFICIAL DIET MIXED WITH EXTRACT UF 5 5FECIi

ACCuMULATIVE M RTALITY AbTT5 FORMULA) PLANT SPECIES FRACTON CCNC(Pk-H _

DAY 1 3 4 5 6 7 a 9 20

Aalaia cQrora 3 QCtC H C CI 31) 10000 000 000 000 OOu CO0 000 000 000 000 000

8000 000 000 000 000 000 OOu 00a 000 0O O

6000 334 334 334 334 334 334 334 334 334 334

4000 000 000 OOu 000 000 000 C00 000 000 000

2000 000 0Ou 000 000 010 000 000 000 000 000

41 QC(CHClI I0000 000 000 10000 - - - - - - -

8000 000 000 10000 - - - -

600C 000 333 9000 10000 - - -

4000 000 000 1334 10000 - - -

2000 000 000 3000 9311 10000 --

S QC HampOH) 10000 334 344 551 10000 - - -

8000 000 000 3000 9311 10000 - -

6000 000 000 6238 9310 10000 - -

2000 000 000 2758 8965 9643 10000 -

MORTALITY OF URA F FED ON ARTIFICIAL DIET MIXED WITH EATRACT Ok 6 SPECIES

ACCUMULATIVE I HCRTALITY (A-OTT- FORMULA)

PLANT SPECIESI FRACTICH CONC(PPM)

DAY 1 2 3 4 5 b 7 9 10 11

A______ oOH 10000 000 b67 2333 9333 10000 -

50-0 000 333 E66 7

66 67 89B5 9655 10(00

6000 00 000 6000 6b67 6620 9310 9b55 l00 00 - -

4000 000 000 333 333 1667 4462 6207 8276 8276 9655 0000

2000 000 000 333 333 344 689 2413 2658 3792 5555 -

EtOAc (insol) 10000 000 000 000 000 000 000 344 2758 3447 5172 -

000 000 000 000 030 000 OOG 344 20 EL 20E3 2758 -

6000 000 J00 000 000 000 000 3 44 344 10J4 IJ4 -

4000 000 0 00 000 0 00 000 00 0 00 0 0 000 000 -

2000 000 000 000 000 000 000 OCG 000 000 000 -

tOAc (601) 10000 000 034 4E27 a6 55 100 00 - - - - -

6000 000 689 5517 C000 - - -

6000 000 687 2758 965 10000 - -

4000 000 000 1034 9655 10000 - - -

2000 000 689 2069 6207 9365 10000 - - -

MORTALITY OF F FD ON AKTIFICIAL -4rLXFD WITH LtTPACT CF __ _pCI_

A ACCUNfULATIvE HCRTALI7Y (ABc-S pOprW LA)

Day 1 3 4 5 6 7 9 10 11 12 13

tCCl 30

600

400

200

000

000

000

000

000

000

000

000

000

500

000

000

500

500

000

000

3500

I000

000

000

6500

1100

500

000

9000

20c0

000

000

9500

3000

3000

500

10000

3530

4000

500

-

6000

4300

1500

-

6000

4000

1500

6500

4000

1500

7500

4000

1500

-HGOA Soo

So0

40

200

000

000

000

000

000

500

000

000

000

500

500

000

000

500

500

000

500

500

500

000

500

500

Scd

0clo

500

500

500

000

500

soo

500

000

500

500

500

000

500

500

500

000

500

500

500

000

10500

500

500

500

000

1500

500

500

o00

000

13 14 15 1 b 17 18 19 20 21 22 23 _4 25 26 27 28 9 30

3

3

3

75 00

40 00

1500

7500

-000

1000

8421

7894

1578

8947

8421

2631

9847

8947

3157

8947

9473

3157

8947

S473

8157

9473

9473

7b84

10000 -

9473 9473

421 4210

--

10000

5263 5263

-

5263 5263

-

- - -

3 500

5 00

C(00

1500

500

50000

000

1500

1000

500

000

3000

1000

500

000

4000

1000

500

000

400

1000

500

000

4000

1000

500

000

4000

000

500

000

5000

10 10

500

000

5500

1000

500

500

5500

1000

500

500

6000

1000

500

500

6500

1000

500

500

7000

1000

500

500

7000

1000

500

500

8000

1000

500

500

BO00

2500

500

500

8500

3000

500

500

TABLE

MORTALITY OF 2- LIMamp F FED ON ARTIFICIAL DrT 4 Pr 15WITH EXT ACT3F

PLANT SPECIES FRACTION

I

CONC (PPM)

Day 1

ACCUHULA2IL

3 4

HORTALITY

5

iAbbOT

b

5 FOPHULA)

7 9

holaiA andaani1 L C M 10000

8000

000

000

000

000

000

000

333

333

3 33

356

3j3

3 56

3 33

356

333

356

33

356

333

356

6000 000 000 000 000 000 000 000 0 U0 000 000

lCl

4000

2000

10000

000

000

000

000

000

000

000

000

333

000

000

1000

000

nO0

1071

000

( Go

1071

000

000

o 71

000

000

071

000

000

i01

000

0 o0

10 71

0000

6000

000

000

000

0uO

000

000

1667

0 0

16 67

000

1667

000

16b7

000

1b67

000

1 7

000

1667

000

4000 0O r

000 000 000 000 000 000 0 00 300 000

2000 000 000 0 00 000 000 000 O00 000 000 000

1ON10000

8000

000

000

000

000

333

000

1000

000

1334

000

1334

000

2000

000

2334

333

2334

333

2334

333

6000

4000

000

000

000

000

000

000

000

000

000

300

0 00

000

000

000

333

00G

333

0

333

2000 000 000 000 000 000 000 0 00 000 000 000

TMALhE i

tKDR-ALITY 0 S-L F FED ON ARTFICIAL DIET MIXED WITH Z TCT Ot AGIpound PECIES

ACCUV2-LTIVE 1-7RTALITY BABOf- ORMULA

PLA14T SPECIE5 FRACTICH o (PPM)

Djy I3 4 7 3 10

ampalsajZ C H 10000 000 0 00 000 000 0UO C00 000 010 000 000

8000 333 333 333 333 333 333 333 333 333 333

6000 000 000 000 000 000 000 000 0C 000 333

4000 000 000 000 000 000 000 000 00 0 00 000

2000 000 000 000 000 000 000 000 000 000 000

CHCl 10000 000 000 000 2000 6333 7000 6333 9333 10000 -

2000 000 00-0 000 333 4333 6333 n3 33 9667 10000 -

6000 000 000 D00 1667 2333 4-07 6000 667 332 966

4000 000 000 333 1000 2667 4333 1239000 i66b 10000

2000 000 000 333 1333 20CO 2000 b 00u 7333 d3 3^ 933

MaOH 10000 000 000 333 1000 2333 5333 7000 8333 86 67 9333

8000 333 333 667 1667 3667 5000 6000 -000 3 33 9333

6000 000 000 000 667 1000 3000 4667 70 00 8333 9667

4000 000 000 000 000 333 1000 2333 3 67 60 3u 3 13

2000 000 000 333 333 333 666 2000 4000 4b6 766

TABE I

MORTALITY OF 5_LIrUF F FED ON ARTIFICIAL DIET MIXED WITH EXTRACT OF A SPECES

ACCUMULATIVE- MORTALITY (ABBO--5 FOFLULAI

PLANT SPECIES I FRACTION CONC (PPr)

Day 1 2 3 4 5 6 7 8 9 10

S___ CH 10000 000 000 000 000 000 000 000 6b 7

667 67

8000 000 000 000 000 Cooo 000 000 0 00 0 0o 0 00

6000 000 000 000 000 000 000 000 0 00 000 000

4000 000 000 000 000 000 000 000 000 000 000

2000 000 000 000 000 000 000 000 000 000 000

CHC1 10000 000 000 000 000 000 000 000 000 000 000

8000 000 000 000 000 000 000 000 000 CO0 000

60uo 000 000 000 000 000 00 00 0 01) 000c 0 00

4000 000 000 000 000 000 000 u00 000 000 010

20v0 000 000 000 000 000 000 000 000 000 000

10000 000 000 000 000 000 000 OQO 3O 00c Oc0

8000 000 000 000 000 000 000 000 OCO 00 000

6000 000 000 000 000 000 000 00A 000 000 00

4000 000 000 000 000 000 000 000 000 0 Ou 000

2000 000 000 000 000 000 000 000 000 000 003

TABLE I

MORTALITY OF I U F FE ON ARTIFICIAL DIET MIXED WITH ExTRFCT OF n SPECIES

CONC ACCUMULATIVE MORTALITY (ABBOTS FIRIULA)

PLANT 5PECI2- FRACTION (PPM)

Day 1 2 3 4 5 0 7 8 9 10

pygnxuIm RZ2=L 1rC H 10000 000 000 000 000 000 000 000 666 666 1667

8000 000 000 000 000 000 000 000 666 1000 2000

6000 000 000 OO 000 0 00 000 333 667 1000 2667

CHCl 10000 000 000 000 000 000 000 000 333 333 333

8000 ocO 000 000 000 000 000 000 333 333 333

6000 000 000 000 000 000 000 000 333 1667 2333

- cryit l 10000 000 000 000 000 00m 000 333 667 667 1667

8000 000 00L 000 000 000 333 33 b67 667 l6b7

6000 000 000 000 000 000 030 000 333 333 1000

21non-crystAl 10000 000 000 000 000 000 000 1000 1000 1667 1667

8000 000 000 000 000 000 000 000 000 000 000

K MoOH

6000

10000

000

OO

000

000

OjU

000

000

000

000

000

000

000

000

1333

333

1333

1000

1333

1333

1333

80L0 000 000 000 000 0 G0 000 000 000 000 000

6000 000 000 000 333 333 667 667 667 667 667

MORTALITY OF URA F FED ON ARTIFICIAL DIET MIXED WITH EATRACT Ok 6 SPECIES

ACCUMULATIVE I HCRTALITY (A-OTT- FORMULA)

PLANT SPECIESI FRACTICH CONC(PPM)

DAY 1 2 3 4 5 b 7 9 10 11

A______ oOH 10000 000 b67 2333 9333 10000 -

50-0 000 333 E66 7

66 67 89B5 9655 10(00

6000 00 000 6000 6b67 6620 9310 9b55 l00 00 - -

4000 000 000 333 333 1667 4462 6207 8276 8276 9655 0000

2000 000 000 333 333 344 689 2413 2658 3792 5555 -

EtOAc (insol) 10000 000 000 000 000 000 000 344 2758 3447 5172 -

000 000 000 000 030 000 OOG 344 20 EL 20E3 2758 -

6000 000 J00 000 000 000 000 3 44 344 10J4 IJ4 -

4000 000 0 00 000 0 00 000 00 0 00 0 0 000 000 -

2000 000 000 000 000 000 000 OCG 000 000 000 -

tOAc (601) 10000 000 034 4E27 a6 55 100 00 - - - - -

6000 000 689 5517 C000 - - -

6000 000 687 2758 965 10000 - -

4000 000 000 1034 9655 10000 - - -

2000 000 689 2069 6207 9365 10000 - - -

MORTALITY OF F FD ON AKTIFICIAL -4rLXFD WITH LtTPACT CF __ _pCI_

A ACCUNfULATIvE HCRTALI7Y (ABc-S pOprW LA)

Day 1 3 4 5 6 7 9 10 11 12 13

tCCl 30

600

400

200

000

000

000

000

000

000

000

000

000

500

000

000

500

500

000

000

3500

I000

000

000

6500

1100

500

000

9000

20c0

000

000

9500

3000

3000

500

10000

3530

4000

500

-

6000

4300

1500

-

6000

4000

1500

6500

4000

1500

7500

4000

1500

-HGOA Soo

So0

40

200

000

000

000

000

000

500

000

000

000

500

500

000

000

500

500

000

500

500

500

000

500

500

Scd

0clo

500

500

500

000

500

soo

500

000

500

500

500

000

500

500

500

000

500

500

500

000

10500

500

500

500

000

1500

500

500

o00

000

13 14 15 1 b 17 18 19 20 21 22 23 _4 25 26 27 28 9 30

3

3

3

75 00

40 00

1500

7500

-000

1000

8421

7894

1578

8947

8421

2631

9847

8947

3157

8947

9473

3157

8947

S473

8157

9473

9473

7b84

10000 -

9473 9473

421 4210

--

10000

5263 5263

-

5263 5263

-

- - -

3 500

5 00

C(00

1500

500

50000

000

1500

1000

500

000

3000

1000

500

000

4000

1000

500

000

400

1000

500

000

4000

1000

500

000

4000

000

500

000

5000

10 10

500

000

5500

1000

500

500

5500

1000

500

500

6000

1000

500

500

6500

1000

500

500

7000

1000

500

500

7000

1000

500

500

8000

1000

500

500

BO00

2500

500

500

8500

3000

500

500

TABLE

MORTALITY OF 2- LIMamp F FED ON ARTIFICIAL DrT 4 Pr 15WITH EXT ACT3F

PLANT SPECIES FRACTION

I

CONC (PPM)

Day 1

ACCUHULA2IL

3 4

HORTALITY

5

iAbbOT

b

5 FOPHULA)

7 9

holaiA andaani1 L C M 10000

8000

000

000

000

000

000

000

333

333

3 33

356

3j3

3 56

3 33

356

333

356

33

356

333

356

6000 000 000 000 000 000 000 000 0 U0 000 000

lCl

4000

2000

10000

000

000

000

000

000

000

000

000

333

000

000

1000

000

nO0

1071

000

( Go

1071

000

000

o 71

000

000

071

000

000

i01

000

0 o0

10 71

0000

6000

000

000

000

0uO

000

000

1667

0 0

16 67

000

1667

000

16b7

000

1b67

000

1 7

000

1667

000

4000 0O r

000 000 000 000 000 000 0 00 300 000

2000 000 000 0 00 000 000 000 O00 000 000 000

1ON10000

8000

000

000

000

000

333

000

1000

000

1334

000

1334

000

2000

000

2334

333

2334

333

2334

333

6000

4000

000

000

000

000

000

000

000

000

000

300

0 00

000

000

000

333

00G

333

0

333

2000 000 000 000 000 000 000 0 00 000 000 000

TMALhE i

tKDR-ALITY 0 S-L F FED ON ARTFICIAL DIET MIXED WITH Z TCT Ot AGIpound PECIES

ACCUV2-LTIVE 1-7RTALITY BABOf- ORMULA

PLA14T SPECIE5 FRACTICH o (PPM)

Djy I3 4 7 3 10

ampalsajZ C H 10000 000 0 00 000 000 0UO C00 000 010 000 000

8000 333 333 333 333 333 333 333 333 333 333

6000 000 000 000 000 000 000 000 0C 000 333

4000 000 000 000 000 000 000 000 00 0 00 000

2000 000 000 000 000 000 000 000 000 000 000

CHCl 10000 000 000 000 2000 6333 7000 6333 9333 10000 -

2000 000 00-0 000 333 4333 6333 n3 33 9667 10000 -

6000 000 000 D00 1667 2333 4-07 6000 667 332 966

4000 000 000 333 1000 2667 4333 1239000 i66b 10000

2000 000 000 333 1333 20CO 2000 b 00u 7333 d3 3^ 933

MaOH 10000 000 000 333 1000 2333 5333 7000 8333 86 67 9333

8000 333 333 667 1667 3667 5000 6000 -000 3 33 9333

6000 000 000 000 667 1000 3000 4667 70 00 8333 9667

4000 000 000 000 000 333 1000 2333 3 67 60 3u 3 13

2000 000 000 333 333 333 666 2000 4000 4b6 766

TABE I

MORTALITY OF 5_LIrUF F FED ON ARTIFICIAL DIET MIXED WITH EXTRACT OF A SPECES

ACCUMULATIVE- MORTALITY (ABBO--5 FOFLULAI

PLANT SPECIES I FRACTION CONC (PPr)

Day 1 2 3 4 5 6 7 8 9 10

S___ CH 10000 000 000 000 000 000 000 000 6b 7

667 67

8000 000 000 000 000 Cooo 000 000 0 00 0 0o 0 00

6000 000 000 000 000 000 000 000 0 00 000 000

4000 000 000 000 000 000 000 000 000 000 000

2000 000 000 000 000 000 000 000 000 000 000

CHC1 10000 000 000 000 000 000 000 000 000 000 000

8000 000 000 000 000 000 000 000 000 CO0 000

60uo 000 000 000 000 000 00 00 0 01) 000c 0 00

4000 000 000 000 000 000 000 u00 000 000 010

20v0 000 000 000 000 000 000 000 000 000 000

10000 000 000 000 000 000 000 OQO 3O 00c Oc0

8000 000 000 000 000 000 000 000 OCO 00 000

6000 000 000 000 000 000 000 00A 000 000 00

4000 000 000 000 000 000 000 000 000 0 Ou 000

2000 000 000 000 000 000 000 000 000 000 003

TABLE I

MORTALITY OF I U F FE ON ARTIFICIAL DIET MIXED WITH ExTRFCT OF n SPECIES

CONC ACCUMULATIVE MORTALITY (ABBOTS FIRIULA)

PLANT 5PECI2- FRACTION (PPM)

Day 1 2 3 4 5 0 7 8 9 10

pygnxuIm RZ2=L 1rC H 10000 000 000 000 000 000 000 000 666 666 1667

8000 000 000 000 000 000 000 000 666 1000 2000

6000 000 000 OO 000 0 00 000 333 667 1000 2667

CHCl 10000 000 000 000 000 000 000 000 333 333 333

8000 ocO 000 000 000 000 000 000 333 333 333

6000 000 000 000 000 000 000 000 333 1667 2333

- cryit l 10000 000 000 000 000 00m 000 333 667 667 1667

8000 000 00L 000 000 000 333 33 b67 667 l6b7

6000 000 000 000 000 000 030 000 333 333 1000

21non-crystAl 10000 000 000 000 000 000 000 1000 1000 1667 1667

8000 000 000 000 000 000 000 000 000 000 000

K MoOH

6000

10000

000

OO

000

000

OjU

000

000

000

000

000

000

000

000

1333

333

1333

1000

1333

1333

1333

80L0 000 000 000 000 0 G0 000 000 000 000 000

6000 000 000 000 333 333 667 667 667 667 667

MORTALITY OF F FD ON AKTIFICIAL -4rLXFD WITH LtTPACT CF __ _pCI_

A ACCUNfULATIvE HCRTALI7Y (ABc-S pOprW LA)

Day 1 3 4 5 6 7 9 10 11 12 13

tCCl 30

600

400

200

000

000

000

000

000

000

000

000

000

500

000

000

500

500

000

000

3500

I000

000

000

6500

1100

500

000

9000

20c0

000

000

9500

3000

3000

500

10000

3530

4000

500

-

6000

4300

1500

-

6000

4000

1500

6500

4000

1500

7500

4000

1500

-HGOA Soo

So0

40

200

000

000

000

000

000

500

000

000

000

500

500

000

000

500

500

000

500

500

500

000

500

500

Scd

0clo

500

500

500

000

500

soo

500

000

500

500

500

000

500

500

500

000

500

500

500

000

10500

500

500

500

000

1500

500

500

o00

000

13 14 15 1 b 17 18 19 20 21 22 23 _4 25 26 27 28 9 30

3

3

3

75 00

40 00

1500

7500

-000

1000

8421

7894

1578

8947

8421

2631

9847

8947

3157

8947

9473

3157

8947

S473

8157

9473

9473

7b84

10000 -

9473 9473

421 4210

--

10000

5263 5263

-

5263 5263

-

- - -

3 500

5 00

C(00

1500

500

50000

000

1500

1000

500

000

3000

1000

500

000

4000

1000

500

000

400

1000

500

000

4000

1000

500

000

4000

000

500

000

5000

10 10

500

000

5500

1000

500

500

5500

1000

500

500

6000

1000

500

500

6500

1000

500

500

7000

1000

500

500

7000

1000

500

500

8000

1000

500

500

BO00

2500

500

500

8500

3000

500

500

TABLE

MORTALITY OF 2- LIMamp F FED ON ARTIFICIAL DrT 4 Pr 15WITH EXT ACT3F

PLANT SPECIES FRACTION

I

CONC (PPM)

Day 1

ACCUHULA2IL

3 4

HORTALITY

5

iAbbOT

b

5 FOPHULA)

7 9

holaiA andaani1 L C M 10000

8000

000

000

000

000

000

000

333

333

3 33

356

3j3

3 56

3 33

356

333

356

33

356

333

356

6000 000 000 000 000 000 000 000 0 U0 000 000

lCl

4000

2000

10000

000

000

000

000

000

000

000

000

333

000

000

1000

000

nO0

1071

000

( Go

1071

000

000

o 71

000

000

071

000

000

i01

000

0 o0

10 71

0000

6000

000

000

000

0uO

000

000

1667

0 0

16 67

000

1667

000

16b7

000

1b67

000

1 7

000

1667

000

4000 0O r

000 000 000 000 000 000 0 00 300 000

2000 000 000 0 00 000 000 000 O00 000 000 000

1ON10000

8000

000

000

000

000

333

000

1000

000

1334

000

1334

000

2000

000

2334

333

2334

333

2334

333

6000

4000

000

000

000

000

000

000

000

000

000

300

0 00

000

000

000

333

00G

333

0

333

2000 000 000 000 000 000 000 0 00 000 000 000

TMALhE i

tKDR-ALITY 0 S-L F FED ON ARTFICIAL DIET MIXED WITH Z TCT Ot AGIpound PECIES

ACCUV2-LTIVE 1-7RTALITY BABOf- ORMULA

PLA14T SPECIE5 FRACTICH o (PPM)

Djy I3 4 7 3 10

ampalsajZ C H 10000 000 0 00 000 000 0UO C00 000 010 000 000

8000 333 333 333 333 333 333 333 333 333 333

6000 000 000 000 000 000 000 000 0C 000 333

4000 000 000 000 000 000 000 000 00 0 00 000

2000 000 000 000 000 000 000 000 000 000 000

CHCl 10000 000 000 000 2000 6333 7000 6333 9333 10000 -

2000 000 00-0 000 333 4333 6333 n3 33 9667 10000 -

6000 000 000 D00 1667 2333 4-07 6000 667 332 966

4000 000 000 333 1000 2667 4333 1239000 i66b 10000

2000 000 000 333 1333 20CO 2000 b 00u 7333 d3 3^ 933

MaOH 10000 000 000 333 1000 2333 5333 7000 8333 86 67 9333

8000 333 333 667 1667 3667 5000 6000 -000 3 33 9333

6000 000 000 000 667 1000 3000 4667 70 00 8333 9667

4000 000 000 000 000 333 1000 2333 3 67 60 3u 3 13

2000 000 000 333 333 333 666 2000 4000 4b6 766

TABE I

MORTALITY OF 5_LIrUF F FED ON ARTIFICIAL DIET MIXED WITH EXTRACT OF A SPECES

ACCUMULATIVE- MORTALITY (ABBO--5 FOFLULAI

PLANT SPECIES I FRACTION CONC (PPr)

Day 1 2 3 4 5 6 7 8 9 10

S___ CH 10000 000 000 000 000 000 000 000 6b 7

667 67

8000 000 000 000 000 Cooo 000 000 0 00 0 0o 0 00

6000 000 000 000 000 000 000 000 0 00 000 000

4000 000 000 000 000 000 000 000 000 000 000

2000 000 000 000 000 000 000 000 000 000 000

CHC1 10000 000 000 000 000 000 000 000 000 000 000

8000 000 000 000 000 000 000 000 000 CO0 000

60uo 000 000 000 000 000 00 00 0 01) 000c 0 00

4000 000 000 000 000 000 000 u00 000 000 010

20v0 000 000 000 000 000 000 000 000 000 000

10000 000 000 000 000 000 000 OQO 3O 00c Oc0

8000 000 000 000 000 000 000 000 OCO 00 000

6000 000 000 000 000 000 000 00A 000 000 00

4000 000 000 000 000 000 000 000 000 0 Ou 000

2000 000 000 000 000 000 000 000 000 000 003

TABLE I

MORTALITY OF I U F FE ON ARTIFICIAL DIET MIXED WITH ExTRFCT OF n SPECIES

CONC ACCUMULATIVE MORTALITY (ABBOTS FIRIULA)

PLANT 5PECI2- FRACTION (PPM)

Day 1 2 3 4 5 0 7 8 9 10

pygnxuIm RZ2=L 1rC H 10000 000 000 000 000 000 000 000 666 666 1667

8000 000 000 000 000 000 000 000 666 1000 2000

6000 000 000 OO 000 0 00 000 333 667 1000 2667

CHCl 10000 000 000 000 000 000 000 000 333 333 333

8000 ocO 000 000 000 000 000 000 333 333 333

6000 000 000 000 000 000 000 000 333 1667 2333

- cryit l 10000 000 000 000 000 00m 000 333 667 667 1667

8000 000 00L 000 000 000 333 33 b67 667 l6b7

6000 000 000 000 000 000 030 000 333 333 1000

21non-crystAl 10000 000 000 000 000 000 000 1000 1000 1667 1667

8000 000 000 000 000 000 000 000 000 000 000

K MoOH

6000

10000

000

OO

000

000

OjU

000

000

000

000

000

000

000

000

1333

333

1333

1000

1333

1333

1333

80L0 000 000 000 000 0 G0 000 000 000 000 000

6000 000 000 000 333 333 667 667 667 667 667

13 14 15 1 b 17 18 19 20 21 22 23 _4 25 26 27 28 9 30

3

3

3

75 00

40 00

1500

7500

-000

1000

8421

7894

1578

8947

8421

2631

9847

8947

3157

8947

9473

3157

8947

S473

8157

9473

9473

7b84

10000 -

9473 9473

421 4210

--

10000

5263 5263

-

5263 5263

-

- - -

3 500

5 00

C(00

1500

500

50000

000

1500

1000

500

000

3000

1000

500

000

4000

1000

500

000

400

1000

500

000

4000

1000

500

000

4000

000

500

000

5000

10 10

500

000

5500

1000

500

500

5500

1000

500

500

6000

1000

500

500

6500

1000

500

500

7000

1000

500

500

7000

1000

500

500

8000

1000

500

500

BO00

2500

500

500

8500

3000

500

500

TABLE

MORTALITY OF 2- LIMamp F FED ON ARTIFICIAL DrT 4 Pr 15WITH EXT ACT3F

PLANT SPECIES FRACTION

I

CONC (PPM)

Day 1

ACCUHULA2IL

3 4

HORTALITY

5

iAbbOT

b

5 FOPHULA)

7 9

holaiA andaani1 L C M 10000

8000

000

000

000

000

000

000

333

333

3 33

356

3j3

3 56

3 33

356

333

356

33

356

333

356

6000 000 000 000 000 000 000 000 0 U0 000 000

lCl

4000

2000

10000

000

000

000

000

000

000

000

000

333

000

000

1000

000

nO0

1071

000

( Go

1071

000

000

o 71

000

000

071

000

000

i01

000

0 o0

10 71

0000

6000

000

000

000

0uO

000

000

1667

0 0

16 67

000

1667

000

16b7

000

1b67

000

1 7

000

1667

000

4000 0O r

000 000 000 000 000 000 0 00 300 000

2000 000 000 0 00 000 000 000 O00 000 000 000

1ON10000

8000

000

000

000

000

333

000

1000

000

1334

000

1334

000

2000

000

2334

333

2334

333

2334

333

6000

4000

000

000

000

000

000

000

000

000

000

300

0 00

000

000

000

333

00G

333

0

333

2000 000 000 000 000 000 000 0 00 000 000 000

TMALhE i

tKDR-ALITY 0 S-L F FED ON ARTFICIAL DIET MIXED WITH Z TCT Ot AGIpound PECIES

ACCUV2-LTIVE 1-7RTALITY BABOf- ORMULA

PLA14T SPECIE5 FRACTICH o (PPM)

Djy I3 4 7 3 10

ampalsajZ C H 10000 000 0 00 000 000 0UO C00 000 010 000 000

8000 333 333 333 333 333 333 333 333 333 333

6000 000 000 000 000 000 000 000 0C 000 333

4000 000 000 000 000 000 000 000 00 0 00 000

2000 000 000 000 000 000 000 000 000 000 000

CHCl 10000 000 000 000 2000 6333 7000 6333 9333 10000 -

2000 000 00-0 000 333 4333 6333 n3 33 9667 10000 -

6000 000 000 D00 1667 2333 4-07 6000 667 332 966

4000 000 000 333 1000 2667 4333 1239000 i66b 10000

2000 000 000 333 1333 20CO 2000 b 00u 7333 d3 3^ 933

MaOH 10000 000 000 333 1000 2333 5333 7000 8333 86 67 9333

8000 333 333 667 1667 3667 5000 6000 -000 3 33 9333

6000 000 000 000 667 1000 3000 4667 70 00 8333 9667

4000 000 000 000 000 333 1000 2333 3 67 60 3u 3 13

2000 000 000 333 333 333 666 2000 4000 4b6 766

TABE I

MORTALITY OF 5_LIrUF F FED ON ARTIFICIAL DIET MIXED WITH EXTRACT OF A SPECES

ACCUMULATIVE- MORTALITY (ABBO--5 FOFLULAI

PLANT SPECIES I FRACTION CONC (PPr)

Day 1 2 3 4 5 6 7 8 9 10

S___ CH 10000 000 000 000 000 000 000 000 6b 7

667 67

8000 000 000 000 000 Cooo 000 000 0 00 0 0o 0 00

6000 000 000 000 000 000 000 000 0 00 000 000

4000 000 000 000 000 000 000 000 000 000 000

2000 000 000 000 000 000 000 000 000 000 000

CHC1 10000 000 000 000 000 000 000 000 000 000 000

8000 000 000 000 000 000 000 000 000 CO0 000

60uo 000 000 000 000 000 00 00 0 01) 000c 0 00

4000 000 000 000 000 000 000 u00 000 000 010

20v0 000 000 000 000 000 000 000 000 000 000

10000 000 000 000 000 000 000 OQO 3O 00c Oc0

8000 000 000 000 000 000 000 000 OCO 00 000

6000 000 000 000 000 000 000 00A 000 000 00

4000 000 000 000 000 000 000 000 000 0 Ou 000

2000 000 000 000 000 000 000 000 000 000 003

TABLE I

MORTALITY OF I U F FE ON ARTIFICIAL DIET MIXED WITH ExTRFCT OF n SPECIES

CONC ACCUMULATIVE MORTALITY (ABBOTS FIRIULA)

PLANT 5PECI2- FRACTION (PPM)

Day 1 2 3 4 5 0 7 8 9 10

pygnxuIm RZ2=L 1rC H 10000 000 000 000 000 000 000 000 666 666 1667

8000 000 000 000 000 000 000 000 666 1000 2000

6000 000 000 OO 000 0 00 000 333 667 1000 2667

CHCl 10000 000 000 000 000 000 000 000 333 333 333

8000 ocO 000 000 000 000 000 000 333 333 333

6000 000 000 000 000 000 000 000 333 1667 2333

- cryit l 10000 000 000 000 000 00m 000 333 667 667 1667

8000 000 00L 000 000 000 333 33 b67 667 l6b7

6000 000 000 000 000 000 030 000 333 333 1000

21non-crystAl 10000 000 000 000 000 000 000 1000 1000 1667 1667

8000 000 000 000 000 000 000 000 000 000 000

K MoOH

6000

10000

000

OO

000

000

OjU

000

000

000

000

000

000

000

000

1333

333

1333

1000

1333

1333

1333

80L0 000 000 000 000 0 G0 000 000 000 000 000

6000 000 000 000 333 333 667 667 667 667 667

TABLE

MORTALITY OF 2- LIMamp F FED ON ARTIFICIAL DrT 4 Pr 15WITH EXT ACT3F

PLANT SPECIES FRACTION

I

CONC (PPM)

Day 1

ACCUHULA2IL

3 4

HORTALITY

5

iAbbOT

b

5 FOPHULA)

7 9

holaiA andaani1 L C M 10000

8000

000

000

000

000

000

000

333

333

3 33

356

3j3

3 56

3 33

356

333

356

33

356

333

356

6000 000 000 000 000 000 000 000 0 U0 000 000

lCl

4000

2000

10000

000

000

000

000

000

000

000

000

333

000

000

1000

000

nO0

1071

000

( Go

1071

000

000

o 71

000

000

071

000

000

i01

000

0 o0

10 71

0000

6000

000

000

000

0uO

000

000

1667

0 0

16 67

000

1667

000

16b7

000

1b67

000

1 7

000

1667

000

4000 0O r

000 000 000 000 000 000 0 00 300 000

2000 000 000 0 00 000 000 000 O00 000 000 000

1ON10000

8000

000

000

000

000

333

000

1000

000

1334

000

1334

000

2000

000

2334

333

2334

333

2334

333

6000

4000

000

000

000

000

000

000

000

000

000

300

0 00

000

000

000

333

00G

333

0

333

2000 000 000 000 000 000 000 0 00 000 000 000

TMALhE i

tKDR-ALITY 0 S-L F FED ON ARTFICIAL DIET MIXED WITH Z TCT Ot AGIpound PECIES

ACCUV2-LTIVE 1-7RTALITY BABOf- ORMULA

PLA14T SPECIE5 FRACTICH o (PPM)

Djy I3 4 7 3 10

ampalsajZ C H 10000 000 0 00 000 000 0UO C00 000 010 000 000

8000 333 333 333 333 333 333 333 333 333 333

6000 000 000 000 000 000 000 000 0C 000 333

4000 000 000 000 000 000 000 000 00 0 00 000

2000 000 000 000 000 000 000 000 000 000 000

CHCl 10000 000 000 000 2000 6333 7000 6333 9333 10000 -

2000 000 00-0 000 333 4333 6333 n3 33 9667 10000 -

6000 000 000 D00 1667 2333 4-07 6000 667 332 966

4000 000 000 333 1000 2667 4333 1239000 i66b 10000

2000 000 000 333 1333 20CO 2000 b 00u 7333 d3 3^ 933

MaOH 10000 000 000 333 1000 2333 5333 7000 8333 86 67 9333

8000 333 333 667 1667 3667 5000 6000 -000 3 33 9333

6000 000 000 000 667 1000 3000 4667 70 00 8333 9667

4000 000 000 000 000 333 1000 2333 3 67 60 3u 3 13

2000 000 000 333 333 333 666 2000 4000 4b6 766

TABE I

MORTALITY OF 5_LIrUF F FED ON ARTIFICIAL DIET MIXED WITH EXTRACT OF A SPECES

ACCUMULATIVE- MORTALITY (ABBO--5 FOFLULAI

PLANT SPECIES I FRACTION CONC (PPr)

Day 1 2 3 4 5 6 7 8 9 10

S___ CH 10000 000 000 000 000 000 000 000 6b 7

667 67

8000 000 000 000 000 Cooo 000 000 0 00 0 0o 0 00

6000 000 000 000 000 000 000 000 0 00 000 000

4000 000 000 000 000 000 000 000 000 000 000

2000 000 000 000 000 000 000 000 000 000 000

CHC1 10000 000 000 000 000 000 000 000 000 000 000

8000 000 000 000 000 000 000 000 000 CO0 000

60uo 000 000 000 000 000 00 00 0 01) 000c 0 00

4000 000 000 000 000 000 000 u00 000 000 010

20v0 000 000 000 000 000 000 000 000 000 000

10000 000 000 000 000 000 000 OQO 3O 00c Oc0

8000 000 000 000 000 000 000 000 OCO 00 000

6000 000 000 000 000 000 000 00A 000 000 00

4000 000 000 000 000 000 000 000 000 0 Ou 000

2000 000 000 000 000 000 000 000 000 000 003

TABLE I

MORTALITY OF I U F FE ON ARTIFICIAL DIET MIXED WITH ExTRFCT OF n SPECIES

CONC ACCUMULATIVE MORTALITY (ABBOTS FIRIULA)

PLANT 5PECI2- FRACTION (PPM)

Day 1 2 3 4 5 0 7 8 9 10

pygnxuIm RZ2=L 1rC H 10000 000 000 000 000 000 000 000 666 666 1667

8000 000 000 000 000 000 000 000 666 1000 2000

6000 000 000 OO 000 0 00 000 333 667 1000 2667

CHCl 10000 000 000 000 000 000 000 000 333 333 333

8000 ocO 000 000 000 000 000 000 333 333 333

6000 000 000 000 000 000 000 000 333 1667 2333

- cryit l 10000 000 000 000 000 00m 000 333 667 667 1667

8000 000 00L 000 000 000 333 33 b67 667 l6b7

6000 000 000 000 000 000 030 000 333 333 1000

21non-crystAl 10000 000 000 000 000 000 000 1000 1000 1667 1667

8000 000 000 000 000 000 000 000 000 000 000

K MoOH

6000

10000

000

OO

000

000

OjU

000

000

000

000

000

000

000

000

1333

333

1333

1000

1333

1333

1333

80L0 000 000 000 000 0 G0 000 000 000 000 000

6000 000 000 000 333 333 667 667 667 667 667

TMALhE i

tKDR-ALITY 0 S-L F FED ON ARTFICIAL DIET MIXED WITH Z TCT Ot AGIpound PECIES

ACCUV2-LTIVE 1-7RTALITY BABOf- ORMULA

PLA14T SPECIE5 FRACTICH o (PPM)

Djy I3 4 7 3 10

ampalsajZ C H 10000 000 0 00 000 000 0UO C00 000 010 000 000

8000 333 333 333 333 333 333 333 333 333 333

6000 000 000 000 000 000 000 000 0C 000 333

4000 000 000 000 000 000 000 000 00 0 00 000

2000 000 000 000 000 000 000 000 000 000 000

CHCl 10000 000 000 000 2000 6333 7000 6333 9333 10000 -

2000 000 00-0 000 333 4333 6333 n3 33 9667 10000 -

6000 000 000 D00 1667 2333 4-07 6000 667 332 966

4000 000 000 333 1000 2667 4333 1239000 i66b 10000

2000 000 000 333 1333 20CO 2000 b 00u 7333 d3 3^ 933

MaOH 10000 000 000 333 1000 2333 5333 7000 8333 86 67 9333

8000 333 333 667 1667 3667 5000 6000 -000 3 33 9333

6000 000 000 000 667 1000 3000 4667 70 00 8333 9667

4000 000 000 000 000 333 1000 2333 3 67 60 3u 3 13

2000 000 000 333 333 333 666 2000 4000 4b6 766

TABE I

MORTALITY OF 5_LIrUF F FED ON ARTIFICIAL DIET MIXED WITH EXTRACT OF A SPECES

ACCUMULATIVE- MORTALITY (ABBO--5 FOFLULAI

PLANT SPECIES I FRACTION CONC (PPr)

Day 1 2 3 4 5 6 7 8 9 10

S___ CH 10000 000 000 000 000 000 000 000 6b 7

667 67

8000 000 000 000 000 Cooo 000 000 0 00 0 0o 0 00

6000 000 000 000 000 000 000 000 0 00 000 000

4000 000 000 000 000 000 000 000 000 000 000

2000 000 000 000 000 000 000 000 000 000 000

CHC1 10000 000 000 000 000 000 000 000 000 000 000

8000 000 000 000 000 000 000 000 000 CO0 000

60uo 000 000 000 000 000 00 00 0 01) 000c 0 00

4000 000 000 000 000 000 000 u00 000 000 010

20v0 000 000 000 000 000 000 000 000 000 000

10000 000 000 000 000 000 000 OQO 3O 00c Oc0

8000 000 000 000 000 000 000 000 OCO 00 000

6000 000 000 000 000 000 000 00A 000 000 00

4000 000 000 000 000 000 000 000 000 0 Ou 000

2000 000 000 000 000 000 000 000 000 000 003

TABLE I

MORTALITY OF I U F FE ON ARTIFICIAL DIET MIXED WITH ExTRFCT OF n SPECIES

CONC ACCUMULATIVE MORTALITY (ABBOTS FIRIULA)

PLANT 5PECI2- FRACTION (PPM)

Day 1 2 3 4 5 0 7 8 9 10

pygnxuIm RZ2=L 1rC H 10000 000 000 000 000 000 000 000 666 666 1667

8000 000 000 000 000 000 000 000 666 1000 2000

6000 000 000 OO 000 0 00 000 333 667 1000 2667

CHCl 10000 000 000 000 000 000 000 000 333 333 333

8000 ocO 000 000 000 000 000 000 333 333 333

6000 000 000 000 000 000 000 000 333 1667 2333

- cryit l 10000 000 000 000 000 00m 000 333 667 667 1667

8000 000 00L 000 000 000 333 33 b67 667 l6b7

6000 000 000 000 000 000 030 000 333 333 1000

21non-crystAl 10000 000 000 000 000 000 000 1000 1000 1667 1667

8000 000 000 000 000 000 000 000 000 000 000

K MoOH

6000

10000

000

OO

000

000

OjU

000

000

000

000

000

000

000

000

1333

333

1333

1000

1333

1333

1333

80L0 000 000 000 000 0 G0 000 000 000 000 000

6000 000 000 000 333 333 667 667 667 667 667

TABE I

MORTALITY OF 5_LIrUF F FED ON ARTIFICIAL DIET MIXED WITH EXTRACT OF A SPECES

ACCUMULATIVE- MORTALITY (ABBO--5 FOFLULAI

PLANT SPECIES I FRACTION CONC (PPr)

Day 1 2 3 4 5 6 7 8 9 10

S___ CH 10000 000 000 000 000 000 000 000 6b 7

667 67

8000 000 000 000 000 Cooo 000 000 0 00 0 0o 0 00

6000 000 000 000 000 000 000 000 0 00 000 000

4000 000 000 000 000 000 000 000 000 000 000

2000 000 000 000 000 000 000 000 000 000 000

CHC1 10000 000 000 000 000 000 000 000 000 000 000

8000 000 000 000 000 000 000 000 000 CO0 000

60uo 000 000 000 000 000 00 00 0 01) 000c 0 00

4000 000 000 000 000 000 000 u00 000 000 010

20v0 000 000 000 000 000 000 000 000 000 000

10000 000 000 000 000 000 000 OQO 3O 00c Oc0

8000 000 000 000 000 000 000 000 OCO 00 000

6000 000 000 000 000 000 000 00A 000 000 00

4000 000 000 000 000 000 000 000 000 0 Ou 000

2000 000 000 000 000 000 000 000 000 000 003

TABLE I

MORTALITY OF I U F FE ON ARTIFICIAL DIET MIXED WITH ExTRFCT OF n SPECIES

CONC ACCUMULATIVE MORTALITY (ABBOTS FIRIULA)

PLANT 5PECI2- FRACTION (PPM)

Day 1 2 3 4 5 0 7 8 9 10

pygnxuIm RZ2=L 1rC H 10000 000 000 000 000 000 000 000 666 666 1667

8000 000 000 000 000 000 000 000 666 1000 2000

6000 000 000 OO 000 0 00 000 333 667 1000 2667

CHCl 10000 000 000 000 000 000 000 000 333 333 333

8000 ocO 000 000 000 000 000 000 333 333 333

6000 000 000 000 000 000 000 000 333 1667 2333

- cryit l 10000 000 000 000 000 00m 000 333 667 667 1667

8000 000 00L 000 000 000 333 33 b67 667 l6b7

6000 000 000 000 000 000 030 000 333 333 1000

21non-crystAl 10000 000 000 000 000 000 000 1000 1000 1667 1667

8000 000 000 000 000 000 000 000 000 000 000

K MoOH

6000

10000

000

OO

000

000

OjU

000

000

000

000

000

000

000

000

1333

333

1333

1000

1333

1333

1333

80L0 000 000 000 000 0 G0 000 000 000 000 000

6000 000 000 000 333 333 667 667 667 667 667

TABLE I

MORTALITY OF I U F FE ON ARTIFICIAL DIET MIXED WITH ExTRFCT OF n SPECIES

CONC ACCUMULATIVE MORTALITY (ABBOTS FIRIULA)

PLANT 5PECI2- FRACTION (PPM)

Day 1 2 3 4 5 0 7 8 9 10

pygnxuIm RZ2=L 1rC H 10000 000 000 000 000 000 000 000 666 666 1667

8000 000 000 000 000 000 000 000 666 1000 2000

6000 000 000 OO 000 0 00 000 333 667 1000 2667

CHCl 10000 000 000 000 000 000 000 000 333 333 333

8000 ocO 000 000 000 000 000 000 333 333 333

6000 000 000 000 000 000 000 000 333 1667 2333

- cryit l 10000 000 000 000 000 00m 000 333 667 667 1667

8000 000 00L 000 000 000 333 33 b67 667 l6b7

6000 000 000 000 000 000 030 000 333 333 1000

21non-crystAl 10000 000 000 000 000 000 000 1000 1000 1667 1667

8000 000 000 000 000 000 000 000 000 000 000

K MoOH

6000

10000

000

OO

000

000

OjU

000

000

000

000

000

000

000

000

1333

333

1333

1000

1333

1333

1333

80L0 000 000 000 000 0 G0 000 000 000 000 000

6000 000 000 000 333 333 667 667 667 667 667

MORTALITY OF _ITUF FED ON ARTIFICIAL VIET hIXED WITH LTACT oF YrXY SPECIES

PLA JT SPECIES FRACrION

P

COHC(PPI) ACCUMULATIVE MORTALIY

________________________________________

Day l 2 3 4 5

tABBOTS FOiKh JLAI

6 7 5 9 1D

iyxlu ap _ CH

CH Cl

10000

9000

6000

10000

333

000

000

000

333

000

000

000

33-

000

000

000

333

000

000

000

333

000

000

000

333

000

000

000

333

000

000

000

333

000

667

000

333

667

667

333

333

1000

1000

333

8000

6000

O00

000

000

000

000

000

000

000

000

000

000

000

OO

000

000

000

000

0 30

000

000

HMOH 10000

2000

000

000

000

333 333

000

333

000

333

000

667

0 00

667

0 00

b67

000

667

000

667

6000 000 000 000 000 000 C00 000 000 000 C00

- 7 -

Separation was carried out isolateto the active constituents

from A odota a using tI sche a hown in 11] 2 From the purified

fraction of high act ivi ty a bisan-id odorine(]) isolated

Its structure w evidence from its R spectra (I1 3) and the

terechemitry w i r~rf irmed 4)n y x-ray crystall ogr phy (111

11 2 - Diagram showing separation scheme for odorine

-- O

Ill 3 - 400 Hlz II -NXR spec trum of odortIne

I11 4 - Crystal itructure of odorine

Lpn rf- It inI th activity of this pure componert no

nf ic wt ant iieein ctivity a obs~ved Therefore this compound

cannot be the active inredient ard may only acts as a synerist in the

active fraction

then tnade to iloLate the minor contiLtuen s

in ti ictive frict ir I in I1 with exteniive chroratopraphy

(quici nlumn chrrn tctrr) sco letin (2) was isolated

further atit-WJ a

0 0

(2)

Prhi substance was confirmed as the active ingredient

since 1007 larval mortality resulted from an LD method using 160 ppm

solotion of thin ubstance

Experint was carried out tc pre-determine the lormulation

andplicat ion mothod for testing the antifeedin efficiency of the

crude extract in the f ield ten thi instar of S Ci tua were left

it itedcoan ech of 4 Vigta tadiaa plants in clay pots Ihe pots were

wrr nderl by water in clay trays to prevent the larvae to leave the

plant lo ts

rud rethanolic extract was admixed with bentonite in 13

by weight 3 parts of bentonite were added to I part of mcthanolic extract

in m hanol the solvent was then evaporated to dryness to give a brown

Pocwd er

An queous emulsion containing 005 Iriton and an equivalent

of 2 c rude methanolic extract wlt s made from the brown powder and sprayed

on tw of the Vina nadiata lat A a control the other two plants

We IV I d I h - - i1t on c rtaihi ton art c oncntratiou of Tiritn

and rtOr it A mrI-prt d i tince itt the I ve dlar pt between the two

(of rt wan h rv(ed W WIC d a b r -rtrnl1 lants were

cc i tt eato up while te treated pl1at reraiwred healthy

f F i ll1)

- 10 shy

111 5 Vigia kad ata 6 days after treatlrent With

crude methanolic extract (treated-coNtrol

tr ated-r-ntrol )

A smai 1 scale field test was then carried out with this

fcrrault ir 150 Vigna adiata p] ants were gron in cement pots

Ili ) After 30 day 300 third intar Iarvv of S -UJia were

l ft t r f It 1IeI i ri each pt (Ill 7) 1he plants in 4 pots

r ra ith If 1- 1d colution (Ill 8) A ii control another

p - r o raved vith n ITeuca (JUtiOn containini the same

cn ctitr a (f etvionit f l Jrilor A crr d diiference in the

v-- - I f din i wi (l 4r it (II 4 I11 )() at 24 hour

Vi a tI oa Ii-t ion of the arei of leaf dama fge and number counts of

r o riti ii a i tit t 4 and Z hrurs after spraying

qiirng tie ft l i++ini or t ul a shy

- 11 -

Calculation of area of leaf damage by S UIuka

leaf = 05) c7 of damage a(5 x + b (5 x 438)(5 x 018)

5x n

= a(25) 119) + c (09) x 100

a -- no of leaves which were d aped (r e than 507deg

b = no of leaves which were da ged ire than 25

c = no of leaves which were damiged maure than 10

(or no of leave coMtinued -pdud spots)with wei

d = no of leaven which wvr less th1r 1A damaged

(or no of lav wIth discontinued dtiraed spots)

a = no of leves which were not damaped

5 approx-- area of 4 cm 2 in which degree of damage was

est i ma ted

n = no of leaves randomly selected for checking in each

treatment

Similar test was also carried out with an aqueous extract

of 10 (ww) fresh leavo and h05 Ariton

The results of both tests were shown in 111 11 and 111 12

- 12 shy

11l 6 - Vigna adiala in cement iot 30 days old

S tua ere left to feed on Vigna kadcta lae

- 13 shy

Spraying With Lutet(I Uon

4shy

iii - Vi-a hadiata 24 hrs after spraying ith

Contro ol ion

10

- 14 shy

--J V ~gtwdiata 24 hr- afteir spraying with tested olution

- 15 -

Ill 1 - Comparison cI feedi ng a reai In

tested and control experiernt

i LE VEI I

0

HlOI4CI

I 12 FFeeding inhibition by tested solution

EDN -INIITO -MPR- OF LI

Q M3

RSH EYES-shy- ( 595

- 16 -

The offf icacy of the Ageaia 6p in crop protection was tested

in t h0 f ar( r plots

EIHld I t 1 111 13

e 10t 19H71 ecember

Place Farmer plots at Han Mae Tae Amphur Sun-Sai Chiang Mai

ro_p Cauliflowers 45 days after seedling transfer

Nubur of larva S MuPims eggs were attached to the cauliflowers

leavcs (Ill 14) After 7 days larvae hatched and grew to

third instar (Ill 15) at which Lime the number of larvae

was counted prior o spraying

Treatrents T l sprayed with control solution 005 Triton in aqueous

sol ut ion

12 sprayed with an tueous suspension containing 8aof

methanolic extract-hentonite (13) rixture and 005 Triton

T3 sprayed with 101 fresh leaf aqueous etract with 005

A i ton

14 sprayed with 10 dry leaf aqueous extract with 005

A1riton

Observation - count aumber rf larvae prior to spraying 12 24 and 48

hours after spraying

Pecsu I - um arized in Ill 16

17shy

11 13

Cauliflowers plots

used in Field Test I

Ill 14 ALtaching S Uulta eggs to cauliflowers leaves

- 18 shy

11 15 - Third instar larvae from the

attached eggs

II 16 - NUR OF INSECT AFER TREATMENT WITH AGLAIA SP

t

7-iV-

ur j$N

ETH

Z

A

H u4

A

CONTROL

A D S O R B E D O N

12

WITHl LEAF MLIIANOI EXTRACT

ADSORBED ON BENTONITE

N-ET

21 Hour

WITHI FRESHi LEAF

NETiIANO EXTRACT

X

48

WITII DRY LEAF

METHtANOL EXTRACT

- 20 -

Field Test 2

Dte 21 February 1988

P1ace Farrrer plot Leside road leading to Wat Sart Luang Chiang Mal

(rop PrBccor c i oI days aft r seedl ing tran sfer

2w _r ( relicatin - 3 rplications of 1 x 3 m plot

Ir 7et T1 prayed with control solution 005Triton in aqueous

solut Ion

T2sprayed with 101 dry leaf aqueous extract with 0057

Triton

T3 sprayed with 10 dry twig aqueous cxtract with 005

1riton

Results summarized in Ill 17 and Ill 18

Ill 17 - NUMBER OF SECT AFTER TREATIMENT W1H AGLAIA SP

I--

II

DAY

CONTROL WITH DRY LEAF WITH DRY TWIG

METHANOL EXTRACI METHANOL EXTRACT

ISI 8 - -LEAF DAAGE AFTER A 7LA i S

3-2i 4

-~~~ ~ ~~~ I-

14 t N

N)

CAA

CONT RO0L t-LTII DRY L AF wl -tiDRY T WG 11EITHANOL EXTRACT 1ITHANOL EXTRACT

- 23 -

Although some degree of efficacy of Agfaia 6p cxtract in

crop prottect on was observed accurate results could not be cheived

dup to the diminishing numbers of larvae both in tie treated and control

experiment

Another uxperiment was then carried out under the Ltter

ccmtr-)Id cond t ions

late 30 March 1988

plac Pon-harvest Centre Chiang Mal University

Sra-l Broccol (25 days after seedling transfer) in clay pot

urrrunded by 1 inch of water in clay tray to prevent the

l rva from escapirng

Nu7yor of I rva 0pot

Pepi arlana -replications of 10 pots

r -r_t II sprayed with control solution 005o Triton in

aqueous solution

2 sprayed with an aqueous emulsion of 2i leaf methanolic

etract and 00O Iriton

T3 prayed with an aqueous emulsion of 2 twig methanolic

extract and 0057 Iriton

Results sumn aried in Ill 19 20 21 22 23

24 -

Ill 19- Compariscn of leaves damage 4 days after

treatment with 27 leaf methanolic extract

iMd Con tro I

Ill 20 Corrp-ison of leaf dami ge 5 days

after treatment with 24 ]eiif

metharnaoIc extract anI conitrol

- 25 shy

1121 - CoPa r I Son of leaf darrage 5 (lays

after trmarment wi th 2 leaf

methanle] ic extrLct (T 2) 2 twig

netha-nclIc extract (T 3 ) and

control (

ATERATM ITH AGLAIA Sp

a a

CONTROL WITh LEAF METHANOL WITH TWIG METHANOL EXTRACT

EXTRACT

i 23 LEA DAA P G- F A - AGLAIA

Uii

-I-I

1 4

ct cl

CONTROL tjITH LEAF METHAINOL

EXTRACTEXTRACT

WITH TI IG M shy THAN O

- 28 shy

RRIEAwaO PLAN

It can be ccn Iuded from Ihe ( iermenLs on Agtaia sp

that thre exI nt a pa qihilIty o usin this plant as an antifeedant

for Srdopten Ulna in agriculture IPlans have been node for the

I 1l1itu i IL

1) Furdther asSess other irrnu latiorn of Agaa 6p in the

field

2) Compare the activity of scopoletin which is the active

antifeedant in thi plant with other analogues

Toward the end of this reporting period we had reassesed

the insecticidol activitv of the plants which had been pieviously

studied and recorded in our earlior report with various forul ations

Pr arable roult r obtained from the prolictinary studies

0 finl period of this project will then be also spent in evaluating

their Tplical ility ira the fields

IlFFpp[ cFE

I Sai Tikwattanit Mc Thesis Kasetsart University(1977)

2Vciha Patuoumotat lSc TIhILis aseLtL rt University

Si172)

5 Rtnolh I a I I h1 Ich~z br t~nt XIAIpp (195f1) 1I209

L an a I 1 I( I) _i 109C

7 thinbaton al LJ Ec-ktt JkrCtem Soc (1912) 94 825d

C i 1 2 a nd Ling JCS

- 29 shy

9 MJBHqluy 2Cruubju 1J lam andc LAWhj t iio JCSChumI

(012 (i12) 1

IU iil 12 ~cit I ~ J03 lnuut Ilivio ( Ijibt) 14 106 3

11 V--1 lni I1~~u Ji)141 L)ad4 -i

1 Al~Kuai NMzkhhi ACuizwk iiI sjuriai Ayr hiil Chem (073) 1y(09

3 K Wda Y Li ato aind FKmnaat Acjr Iiol ChUri 0) (9 34 941

14 EWaija Y Lnuotu and K151Maiio Ag~r ia l Chem (1970) 14 94b

IKatc andu Yjluzaka ta Tet rahedron Le tter (19 74)

17 i v i v a n a d9

(1175) 2 197S

19 KLO2) 1 Miu-a anO C 1iako2j hi 3 CLrCcd(97 6701

20 Iamp Lbo LY-0I KNaLkaishi and ACiiapya JCS

2 1 I ibo SNpjgti Y- Mi ura Nakanislii and AChay

22 Kul~ 1 -4I ( 1 Pet tLe I 1ijwicz and~t~nrih

J C L Cnc1 Coo ) 10 1

23 1 F aboampi F Ha an i sh i T I 1 soh and CillWKa k aW TKuuta

24 7 Iedil Hout- Plan~rt CCC 2 C t-dice to Io it A C S Sympos i Utn

Meriv Uc2 Ameiricajn ChenicoI a00201 gt4 WaiinqLyofl DC (1977)

1 73

PAeIini Plaiin~t JtIC1tacc to l(2tsi CS Stpi r

Series~ U inri can Chemical SOcact washington DC (1977) 374

0

- 30 shy

26 Il Slurkil1 A Dictionary of the Economic Products of the

Hal ay enin-iula 2 VolMs Minitry of Agriculture and Cooprratives

huj ] Lupuira L-urpir (11)(6)

27 H Grair g t i]i I]ant Spc ie Prportedl y Possesing

I(rt-Control Propert ji-An IICUll )atabjaIe Vesource Systems

1nntjtitt irt-~e~ -tCrnter Hn)roluilu (1985)

23 P1 Tuntiwrwh tt ikurl -t al ht chremjftry (1981 )20 1164

29 T Imatayakul -t 11 A t Chrem (1979) 2 71

30 H HitnlI nll j Yrcwoto) Na tura llv Occurring Inecticides ed

7aconon H ann] Croby P( Marcel Dekker New York (1971) 62

31 W Itwers tatural Productf for Innovative Pest Management

ed Whitehead D L and flowers WS Pergqanon Press Oxford

(1903) 52

- 7 -

Separation was carried out isolateto the active constituents

from A odota a using tI sche a hown in 11] 2 From the purified

fraction of high act ivi ty a bisan-id odorine(]) isolated

Its structure w evidence from its R spectra (I1 3) and the

terechemitry w i r~rf irmed 4)n y x-ray crystall ogr phy (111

11 2 - Diagram showing separation scheme for odorine

-- O

Ill 3 - 400 Hlz II -NXR spec trum of odortIne

I11 4 - Crystal itructure of odorine

Lpn rf- It inI th activity of this pure componert no

nf ic wt ant iieein ctivity a obs~ved Therefore this compound

cannot be the active inredient ard may only acts as a synerist in the

active fraction

then tnade to iloLate the minor contiLtuen s

in ti ictive frict ir I in I1 with exteniive chroratopraphy

(quici nlumn chrrn tctrr) sco letin (2) was isolated

further atit-WJ a

0 0

(2)

Prhi substance was confirmed as the active ingredient

since 1007 larval mortality resulted from an LD method using 160 ppm

solotion of thin ubstance

Experint was carried out tc pre-determine the lormulation

andplicat ion mothod for testing the antifeedin efficiency of the

crude extract in the f ield ten thi instar of S Ci tua were left

it itedcoan ech of 4 Vigta tadiaa plants in clay pots Ihe pots were

wrr nderl by water in clay trays to prevent the larvae to leave the

plant lo ts

rud rethanolic extract was admixed with bentonite in 13

by weight 3 parts of bentonite were added to I part of mcthanolic extract

in m hanol the solvent was then evaporated to dryness to give a brown

Pocwd er

An queous emulsion containing 005 Iriton and an equivalent

of 2 c rude methanolic extract wlt s made from the brown powder and sprayed

on tw of the Vina nadiata lat A a control the other two plants

We IV I d I h - - i1t on c rtaihi ton art c oncntratiou of Tiritn

and rtOr it A mrI-prt d i tince itt the I ve dlar pt between the two

(of rt wan h rv(ed W WIC d a b r -rtrnl1 lants were

cc i tt eato up while te treated pl1at reraiwred healthy

f F i ll1)

- 10 shy

111 5 Vigia kad ata 6 days after treatlrent With

crude methanolic extract (treated-coNtrol

tr ated-r-ntrol )

A smai 1 scale field test was then carried out with this

fcrrault ir 150 Vigna adiata p] ants were gron in cement pots

Ili ) After 30 day 300 third intar Iarvv of S -UJia were

l ft t r f It 1IeI i ri each pt (Ill 7) 1he plants in 4 pots

r ra ith If 1- 1d colution (Ill 8) A ii control another

p - r o raved vith n ITeuca (JUtiOn containini the same

cn ctitr a (f etvionit f l Jrilor A crr d diiference in the

v-- - I f din i wi (l 4r it (II 4 I11 )() at 24 hour

Vi a tI oa Ii-t ion of the arei of leaf dama fge and number counts of

r o riti ii a i tit t 4 and Z hrurs after spraying

qiirng tie ft l i++ini or t ul a shy

- 11 -

Calculation of area of leaf damage by S UIuka

leaf = 05) c7 of damage a(5 x + b (5 x 438)(5 x 018)

5x n

= a(25) 119) + c (09) x 100

a -- no of leaves which were d aped (r e than 507deg

b = no of leaves which were da ged ire than 25

c = no of leaves which were damiged maure than 10

(or no of leave coMtinued -pdud spots)with wei

d = no of leaven which wvr less th1r 1A damaged

(or no of lav wIth discontinued dtiraed spots)

a = no of leves which were not damaped

5 approx-- area of 4 cm 2 in which degree of damage was

est i ma ted

n = no of leaves randomly selected for checking in each

treatment

Similar test was also carried out with an aqueous extract

of 10 (ww) fresh leavo and h05 Ariton

The results of both tests were shown in 111 11 and 111 12

- 12 shy

11l 6 - Vigna adiala in cement iot 30 days old

S tua ere left to feed on Vigna kadcta lae

- 13 shy

Spraying With Lutet(I Uon

4shy

iii - Vi-a hadiata 24 hrs after spraying ith

Contro ol ion

10

- 14 shy

--J V ~gtwdiata 24 hr- afteir spraying with tested olution

- 15 -

Ill 1 - Comparison cI feedi ng a reai In

tested and control experiernt

i LE VEI I

0

HlOI4CI

I 12 FFeeding inhibition by tested solution

EDN -INIITO -MPR- OF LI

Q M3

RSH EYES-shy- ( 595

- 16 -

The offf icacy of the Ageaia 6p in crop protection was tested

in t h0 f ar( r plots

EIHld I t 1 111 13

e 10t 19H71 ecember

Place Farmer plots at Han Mae Tae Amphur Sun-Sai Chiang Mai

ro_p Cauliflowers 45 days after seedling transfer

Nubur of larva S MuPims eggs were attached to the cauliflowers

leavcs (Ill 14) After 7 days larvae hatched and grew to

third instar (Ill 15) at which Lime the number of larvae

was counted prior o spraying

Treatrents T l sprayed with control solution 005 Triton in aqueous

sol ut ion

12 sprayed with an tueous suspension containing 8aof

methanolic extract-hentonite (13) rixture and 005 Triton

T3 sprayed with 101 fresh leaf aqueous etract with 005

A i ton

14 sprayed with 10 dry leaf aqueous extract with 005

A1riton

Observation - count aumber rf larvae prior to spraying 12 24 and 48

hours after spraying

Pecsu I - um arized in Ill 16

17shy

11 13

Cauliflowers plots

used in Field Test I

Ill 14 ALtaching S Uulta eggs to cauliflowers leaves

- 18 shy

11 15 - Third instar larvae from the

attached eggs

II 16 - NUR OF INSECT AFER TREATMENT WITH AGLAIA SP

t

7-iV-

ur j$N

ETH

Z

A

H u4

A

CONTROL

A D S O R B E D O N

12

WITHl LEAF MLIIANOI EXTRACT

ADSORBED ON BENTONITE

N-ET

21 Hour

WITHI FRESHi LEAF

NETiIANO EXTRACT

X

48

WITII DRY LEAF

METHtANOL EXTRACT

- 20 -

Field Test 2

Dte 21 February 1988

P1ace Farrrer plot Leside road leading to Wat Sart Luang Chiang Mal

(rop PrBccor c i oI days aft r seedl ing tran sfer

2w _r ( relicatin - 3 rplications of 1 x 3 m plot

Ir 7et T1 prayed with control solution 005Triton in aqueous

solut Ion

T2sprayed with 101 dry leaf aqueous extract with 0057

Triton

T3 sprayed with 10 dry twig aqueous cxtract with 005

1riton

Results summarized in Ill 17 and Ill 18

Ill 17 - NUMBER OF SECT AFTER TREATIMENT W1H AGLAIA SP

I--

II

DAY

CONTROL WITH DRY LEAF WITH DRY TWIG

METHANOL EXTRACI METHANOL EXTRACT

ISI 8 - -LEAF DAAGE AFTER A 7LA i S

3-2i 4

-~~~ ~ ~~~ I-

14 t N

N)

CAA

CONT RO0L t-LTII DRY L AF wl -tiDRY T WG 11EITHANOL EXTRACT 1ITHANOL EXTRACT

- 23 -

Although some degree of efficacy of Agfaia 6p cxtract in

crop prottect on was observed accurate results could not be cheived

dup to the diminishing numbers of larvae both in tie treated and control

experiment

Another uxperiment was then carried out under the Ltter

ccmtr-)Id cond t ions

late 30 March 1988

plac Pon-harvest Centre Chiang Mal University

Sra-l Broccol (25 days after seedling transfer) in clay pot

urrrunded by 1 inch of water in clay tray to prevent the

l rva from escapirng

Nu7yor of I rva 0pot

Pepi arlana -replications of 10 pots

r -r_t II sprayed with control solution 005o Triton in

aqueous solution

2 sprayed with an aqueous emulsion of 2i leaf methanolic

etract and 00O Iriton

T3 prayed with an aqueous emulsion of 2 twig methanolic

extract and 0057 Iriton

Results sumn aried in Ill 19 20 21 22 23

24 -

Ill 19- Compariscn of leaves damage 4 days after

treatment with 27 leaf methanolic extract

iMd Con tro I

Ill 20 Corrp-ison of leaf dami ge 5 days

after treatment with 24 ]eiif

metharnaoIc extract anI conitrol

- 25 shy

1121 - CoPa r I Son of leaf darrage 5 (lays

after trmarment wi th 2 leaf

methanle] ic extrLct (T 2) 2 twig

netha-nclIc extract (T 3 ) and

control (

ATERATM ITH AGLAIA Sp

a a

CONTROL WITh LEAF METHANOL WITH TWIG METHANOL EXTRACT

EXTRACT

i 23 LEA DAA P G- F A - AGLAIA

Uii

-I-I

1 4

ct cl

CONTROL tjITH LEAF METHAINOL

EXTRACTEXTRACT

WITH TI IG M shy THAN O

- 28 shy

RRIEAwaO PLAN

It can be ccn Iuded from Ihe ( iermenLs on Agtaia sp

that thre exI nt a pa qihilIty o usin this plant as an antifeedant

for Srdopten Ulna in agriculture IPlans have been node for the

I 1l1itu i IL

1) Furdther asSess other irrnu latiorn of Agaa 6p in the

field

2) Compare the activity of scopoletin which is the active

antifeedant in thi plant with other analogues

Toward the end of this reporting period we had reassesed

the insecticidol activitv of the plants which had been pieviously

studied and recorded in our earlior report with various forul ations

Pr arable roult r obtained from the prolictinary studies

0 finl period of this project will then be also spent in evaluating

their Tplical ility ira the fields

IlFFpp[ cFE

I Sai Tikwattanit Mc Thesis Kasetsart University(1977)

2Vciha Patuoumotat lSc TIhILis aseLtL rt University

Si172)

5 Rtnolh I a I I h1 Ich~z br t~nt XIAIpp (195f1) 1I209

L an a I 1 I( I) _i 109C

7 thinbaton al LJ Ec-ktt JkrCtem Soc (1912) 94 825d

C i 1 2 a nd Ling JCS

- 29 shy

9 MJBHqluy 2Cruubju 1J lam andc LAWhj t iio JCSChumI

(012 (i12) 1

IU iil 12 ~cit I ~ J03 lnuut Ilivio ( Ijibt) 14 106 3

11 V--1 lni I1~~u Ji)141 L)ad4 -i

1 Al~Kuai NMzkhhi ACuizwk iiI sjuriai Ayr hiil Chem (073) 1y(09

3 K Wda Y Li ato aind FKmnaat Acjr Iiol ChUri 0) (9 34 941

14 EWaija Y Lnuotu and K151Maiio Ag~r ia l Chem (1970) 14 94b

IKatc andu Yjluzaka ta Tet rahedron Le tter (19 74)

17 i v i v a n a d9

(1175) 2 197S

19 KLO2) 1 Miu-a anO C 1iako2j hi 3 CLrCcd(97 6701

20 Iamp Lbo LY-0I KNaLkaishi and ACiiapya JCS

2 1 I ibo SNpjgti Y- Mi ura Nakanislii and AChay

22 Kul~ 1 -4I ( 1 Pet tLe I 1ijwicz and~t~nrih

J C L Cnc1 Coo ) 10 1

23 1 F aboampi F Ha an i sh i T I 1 soh and CillWKa k aW TKuuta

24 7 Iedil Hout- Plan~rt CCC 2 C t-dice to Io it A C S Sympos i Utn

Meriv Uc2 Ameiricajn ChenicoI a00201 gt4 WaiinqLyofl DC (1977)

1 73

PAeIini Plaiin~t JtIC1tacc to l(2tsi CS Stpi r

Series~ U inri can Chemical SOcact washington DC (1977) 374

0

- 30 shy

26 Il Slurkil1 A Dictionary of the Economic Products of the

Hal ay enin-iula 2 VolMs Minitry of Agriculture and Cooprratives

huj ] Lupuira L-urpir (11)(6)

27 H Grair g t i]i I]ant Spc ie Prportedl y Possesing

I(rt-Control Propert ji-An IICUll )atabjaIe Vesource Systems

1nntjtitt irt-~e~ -tCrnter Hn)roluilu (1985)

23 P1 Tuntiwrwh tt ikurl -t al ht chremjftry (1981 )20 1164

29 T Imatayakul -t 11 A t Chrem (1979) 2 71

30 H HitnlI nll j Yrcwoto) Na tura llv Occurring Inecticides ed

7aconon H ann] Croby P( Marcel Dekker New York (1971) 62

31 W Itwers tatural Productf for Innovative Pest Management

ed Whitehead D L and flowers WS Pergqanon Press Oxford

(1903) 52

I11 4 - Crystal itructure of odorine

Lpn rf- It inI th activity of this pure componert no

nf ic wt ant iieein ctivity a obs~ved Therefore this compound

cannot be the active inredient ard may only acts as a synerist in the

active fraction

then tnade to iloLate the minor contiLtuen s

in ti ictive frict ir I in I1 with exteniive chroratopraphy

(quici nlumn chrrn tctrr) sco letin (2) was isolated

further atit-WJ a

0 0

(2)

Prhi substance was confirmed as the active ingredient

since 1007 larval mortality resulted from an LD method using 160 ppm

solotion of thin ubstance

Experint was carried out tc pre-determine the lormulation

andplicat ion mothod for testing the antifeedin efficiency of the

crude extract in the f ield ten thi instar of S Ci tua were left

it itedcoan ech of 4 Vigta tadiaa plants in clay pots Ihe pots were

wrr nderl by water in clay trays to prevent the larvae to leave the

plant lo ts

rud rethanolic extract was admixed with bentonite in 13

by weight 3 parts of bentonite were added to I part of mcthanolic extract

in m hanol the solvent was then evaporated to dryness to give a brown

Pocwd er

An queous emulsion containing 005 Iriton and an equivalent

of 2 c rude methanolic extract wlt s made from the brown powder and sprayed

on tw of the Vina nadiata lat A a control the other two plants

We IV I d I h - - i1t on c rtaihi ton art c oncntratiou of Tiritn

and rtOr it A mrI-prt d i tince itt the I ve dlar pt between the two

(of rt wan h rv(ed W WIC d a b r -rtrnl1 lants were

cc i tt eato up while te treated pl1at reraiwred healthy

f F i ll1)

- 10 shy

111 5 Vigia kad ata 6 days after treatlrent With

crude methanolic extract (treated-coNtrol

tr ated-r-ntrol )

A smai 1 scale field test was then carried out with this

fcrrault ir 150 Vigna adiata p] ants were gron in cement pots

Ili ) After 30 day 300 third intar Iarvv of S -UJia were

l ft t r f It 1IeI i ri each pt (Ill 7) 1he plants in 4 pots

r ra ith If 1- 1d colution (Ill 8) A ii control another

p - r o raved vith n ITeuca (JUtiOn containini the same

cn ctitr a (f etvionit f l Jrilor A crr d diiference in the

v-- - I f din i wi (l 4r it (II 4 I11 )() at 24 hour

Vi a tI oa Ii-t ion of the arei of leaf dama fge and number counts of

r o riti ii a i tit t 4 and Z hrurs after spraying

qiirng tie ft l i++ini or t ul a shy

- 11 -

Calculation of area of leaf damage by S UIuka

leaf = 05) c7 of damage a(5 x + b (5 x 438)(5 x 018)

5x n

= a(25) 119) + c (09) x 100

a -- no of leaves which were d aped (r e than 507deg

b = no of leaves which were da ged ire than 25

c = no of leaves which were damiged maure than 10

(or no of leave coMtinued -pdud spots)with wei

d = no of leaven which wvr less th1r 1A damaged

(or no of lav wIth discontinued dtiraed spots)

a = no of leves which were not damaped

5 approx-- area of 4 cm 2 in which degree of damage was

est i ma ted

n = no of leaves randomly selected for checking in each

treatment

Similar test was also carried out with an aqueous extract

of 10 (ww) fresh leavo and h05 Ariton

The results of both tests were shown in 111 11 and 111 12

- 12 shy

11l 6 - Vigna adiala in cement iot 30 days old

S tua ere left to feed on Vigna kadcta lae

- 13 shy

Spraying With Lutet(I Uon

4shy

iii - Vi-a hadiata 24 hrs after spraying ith

Contro ol ion

10

- 14 shy

--J V ~gtwdiata 24 hr- afteir spraying with tested olution

- 15 -

Ill 1 - Comparison cI feedi ng a reai In

tested and control experiernt

i LE VEI I

0

HlOI4CI

I 12 FFeeding inhibition by tested solution

EDN -INIITO -MPR- OF LI

Q M3

RSH EYES-shy- ( 595

- 16 -

The offf icacy of the Ageaia 6p in crop protection was tested

in t h0 f ar( r plots

EIHld I t 1 111 13

e 10t 19H71 ecember

Place Farmer plots at Han Mae Tae Amphur Sun-Sai Chiang Mai

ro_p Cauliflowers 45 days after seedling transfer

Nubur of larva S MuPims eggs were attached to the cauliflowers

leavcs (Ill 14) After 7 days larvae hatched and grew to

third instar (Ill 15) at which Lime the number of larvae

was counted prior o spraying

Treatrents T l sprayed with control solution 005 Triton in aqueous

sol ut ion

12 sprayed with an tueous suspension containing 8aof

methanolic extract-hentonite (13) rixture and 005 Triton

T3 sprayed with 101 fresh leaf aqueous etract with 005

A i ton

14 sprayed with 10 dry leaf aqueous extract with 005

A1riton

Observation - count aumber rf larvae prior to spraying 12 24 and 48

hours after spraying

Pecsu I - um arized in Ill 16

17shy

11 13

Cauliflowers plots

used in Field Test I

Ill 14 ALtaching S Uulta eggs to cauliflowers leaves

- 18 shy

11 15 - Third instar larvae from the

attached eggs

II 16 - NUR OF INSECT AFER TREATMENT WITH AGLAIA SP

t

7-iV-

ur j$N

ETH

Z

A

H u4

A

CONTROL

A D S O R B E D O N

12

WITHl LEAF MLIIANOI EXTRACT

ADSORBED ON BENTONITE

N-ET

21 Hour

WITHI FRESHi LEAF

NETiIANO EXTRACT

X

48

WITII DRY LEAF

METHtANOL EXTRACT

- 20 -

Field Test 2

Dte 21 February 1988

P1ace Farrrer plot Leside road leading to Wat Sart Luang Chiang Mal

(rop PrBccor c i oI days aft r seedl ing tran sfer

2w _r ( relicatin - 3 rplications of 1 x 3 m plot

Ir 7et T1 prayed with control solution 005Triton in aqueous

solut Ion

T2sprayed with 101 dry leaf aqueous extract with 0057

Triton

T3 sprayed with 10 dry twig aqueous cxtract with 005

1riton

Results summarized in Ill 17 and Ill 18

Ill 17 - NUMBER OF SECT AFTER TREATIMENT W1H AGLAIA SP

I--

II

DAY

CONTROL WITH DRY LEAF WITH DRY TWIG

METHANOL EXTRACI METHANOL EXTRACT

ISI 8 - -LEAF DAAGE AFTER A 7LA i S

3-2i 4

-~~~ ~ ~~~ I-

14 t N

N)

CAA

CONT RO0L t-LTII DRY L AF wl -tiDRY T WG 11EITHANOL EXTRACT 1ITHANOL EXTRACT

- 23 -

Although some degree of efficacy of Agfaia 6p cxtract in

crop prottect on was observed accurate results could not be cheived

dup to the diminishing numbers of larvae both in tie treated and control

experiment

Another uxperiment was then carried out under the Ltter

ccmtr-)Id cond t ions

late 30 March 1988

plac Pon-harvest Centre Chiang Mal University

Sra-l Broccol (25 days after seedling transfer) in clay pot

urrrunded by 1 inch of water in clay tray to prevent the

l rva from escapirng

Nu7yor of I rva 0pot

Pepi arlana -replications of 10 pots

r -r_t II sprayed with control solution 005o Triton in

aqueous solution

2 sprayed with an aqueous emulsion of 2i leaf methanolic

etract and 00O Iriton

T3 prayed with an aqueous emulsion of 2 twig methanolic

extract and 0057 Iriton

Results sumn aried in Ill 19 20 21 22 23

24 -

Ill 19- Compariscn of leaves damage 4 days after

treatment with 27 leaf methanolic extract

iMd Con tro I

Ill 20 Corrp-ison of leaf dami ge 5 days

after treatment with 24 ]eiif

metharnaoIc extract anI conitrol

- 25 shy

1121 - CoPa r I Son of leaf darrage 5 (lays

after trmarment wi th 2 leaf

methanle] ic extrLct (T 2) 2 twig

netha-nclIc extract (T 3 ) and

control (

ATERATM ITH AGLAIA Sp

a a

CONTROL WITh LEAF METHANOL WITH TWIG METHANOL EXTRACT

EXTRACT

i 23 LEA DAA P G- F A - AGLAIA

Uii

-I-I

1 4

ct cl

CONTROL tjITH LEAF METHAINOL

EXTRACTEXTRACT

WITH TI IG M shy THAN O

- 28 shy

RRIEAwaO PLAN

It can be ccn Iuded from Ihe ( iermenLs on Agtaia sp

that thre exI nt a pa qihilIty o usin this plant as an antifeedant

for Srdopten Ulna in agriculture IPlans have been node for the

I 1l1itu i IL

1) Furdther asSess other irrnu latiorn of Agaa 6p in the

field

2) Compare the activity of scopoletin which is the active

antifeedant in thi plant with other analogues

Toward the end of this reporting period we had reassesed

the insecticidol activitv of the plants which had been pieviously

studied and recorded in our earlior report with various forul ations

Pr arable roult r obtained from the prolictinary studies

0 finl period of this project will then be also spent in evaluating

their Tplical ility ira the fields

IlFFpp[ cFE

I Sai Tikwattanit Mc Thesis Kasetsart University(1977)

2Vciha Patuoumotat lSc TIhILis aseLtL rt University

Si172)

5 Rtnolh I a I I h1 Ich~z br t~nt XIAIpp (195f1) 1I209

L an a I 1 I( I) _i 109C

7 thinbaton al LJ Ec-ktt JkrCtem Soc (1912) 94 825d

C i 1 2 a nd Ling JCS

- 29 shy

9 MJBHqluy 2Cruubju 1J lam andc LAWhj t iio JCSChumI

(012 (i12) 1

IU iil 12 ~cit I ~ J03 lnuut Ilivio ( Ijibt) 14 106 3

11 V--1 lni I1~~u Ji)141 L)ad4 -i

1 Al~Kuai NMzkhhi ACuizwk iiI sjuriai Ayr hiil Chem (073) 1y(09

3 K Wda Y Li ato aind FKmnaat Acjr Iiol ChUri 0) (9 34 941

14 EWaija Y Lnuotu and K151Maiio Ag~r ia l Chem (1970) 14 94b

IKatc andu Yjluzaka ta Tet rahedron Le tter (19 74)

17 i v i v a n a d9

(1175) 2 197S

19 KLO2) 1 Miu-a anO C 1iako2j hi 3 CLrCcd(97 6701

20 Iamp Lbo LY-0I KNaLkaishi and ACiiapya JCS

2 1 I ibo SNpjgti Y- Mi ura Nakanislii and AChay

22 Kul~ 1 -4I ( 1 Pet tLe I 1ijwicz and~t~nrih

J C L Cnc1 Coo ) 10 1

23 1 F aboampi F Ha an i sh i T I 1 soh and CillWKa k aW TKuuta

24 7 Iedil Hout- Plan~rt CCC 2 C t-dice to Io it A C S Sympos i Utn

Meriv Uc2 Ameiricajn ChenicoI a00201 gt4 WaiinqLyofl DC (1977)

1 73

PAeIini Plaiin~t JtIC1tacc to l(2tsi CS Stpi r

Series~ U inri can Chemical SOcact washington DC (1977) 374

0

- 30 shy

26 Il Slurkil1 A Dictionary of the Economic Products of the

Hal ay enin-iula 2 VolMs Minitry of Agriculture and Cooprratives

huj ] Lupuira L-urpir (11)(6)

27 H Grair g t i]i I]ant Spc ie Prportedl y Possesing

I(rt-Control Propert ji-An IICUll )atabjaIe Vesource Systems

1nntjtitt irt-~e~ -tCrnter Hn)roluilu (1985)

23 P1 Tuntiwrwh tt ikurl -t al ht chremjftry (1981 )20 1164

29 T Imatayakul -t 11 A t Chrem (1979) 2 71

30 H HitnlI nll j Yrcwoto) Na tura llv Occurring Inecticides ed

7aconon H ann] Croby P( Marcel Dekker New York (1971) 62

31 W Itwers tatural Productf for Innovative Pest Management

ed Whitehead D L and flowers WS Pergqanon Press Oxford

(1903) 52

0 0

(2)

Prhi substance was confirmed as the active ingredient

since 1007 larval mortality resulted from an LD method using 160 ppm

solotion of thin ubstance

Experint was carried out tc pre-determine the lormulation

andplicat ion mothod for testing the antifeedin efficiency of the

crude extract in the f ield ten thi instar of S Ci tua were left

it itedcoan ech of 4 Vigta tadiaa plants in clay pots Ihe pots were

wrr nderl by water in clay trays to prevent the larvae to leave the

plant lo ts

rud rethanolic extract was admixed with bentonite in 13

by weight 3 parts of bentonite were added to I part of mcthanolic extract

in m hanol the solvent was then evaporated to dryness to give a brown

Pocwd er

An queous emulsion containing 005 Iriton and an equivalent

of 2 c rude methanolic extract wlt s made from the brown powder and sprayed

on tw of the Vina nadiata lat A a control the other two plants

We IV I d I h - - i1t on c rtaihi ton art c oncntratiou of Tiritn

and rtOr it A mrI-prt d i tince itt the I ve dlar pt between the two

(of rt wan h rv(ed W WIC d a b r -rtrnl1 lants were

cc i tt eato up while te treated pl1at reraiwred healthy

f F i ll1)

- 10 shy

111 5 Vigia kad ata 6 days after treatlrent With

crude methanolic extract (treated-coNtrol

tr ated-r-ntrol )

A smai 1 scale field test was then carried out with this

fcrrault ir 150 Vigna adiata p] ants were gron in cement pots

Ili ) After 30 day 300 third intar Iarvv of S -UJia were

l ft t r f It 1IeI i ri each pt (Ill 7) 1he plants in 4 pots

r ra ith If 1- 1d colution (Ill 8) A ii control another

p - r o raved vith n ITeuca (JUtiOn containini the same

cn ctitr a (f etvionit f l Jrilor A crr d diiference in the

v-- - I f din i wi (l 4r it (II 4 I11 )() at 24 hour

Vi a tI oa Ii-t ion of the arei of leaf dama fge and number counts of

r o riti ii a i tit t 4 and Z hrurs after spraying

qiirng tie ft l i++ini or t ul a shy

- 11 -

Calculation of area of leaf damage by S UIuka

leaf = 05) c7 of damage a(5 x + b (5 x 438)(5 x 018)

5x n

= a(25) 119) + c (09) x 100

a -- no of leaves which were d aped (r e than 507deg

b = no of leaves which were da ged ire than 25

c = no of leaves which were damiged maure than 10

(or no of leave coMtinued -pdud spots)with wei

d = no of leaven which wvr less th1r 1A damaged

(or no of lav wIth discontinued dtiraed spots)

a = no of leves which were not damaped

5 approx-- area of 4 cm 2 in which degree of damage was

est i ma ted

n = no of leaves randomly selected for checking in each

treatment

Similar test was also carried out with an aqueous extract

of 10 (ww) fresh leavo and h05 Ariton

The results of both tests were shown in 111 11 and 111 12

- 12 shy

11l 6 - Vigna adiala in cement iot 30 days old

S tua ere left to feed on Vigna kadcta lae

- 13 shy

Spraying With Lutet(I Uon

4shy

iii - Vi-a hadiata 24 hrs after spraying ith

Contro ol ion

10

- 14 shy

--J V ~gtwdiata 24 hr- afteir spraying with tested olution

- 15 -

Ill 1 - Comparison cI feedi ng a reai In

tested and control experiernt

i LE VEI I

0

HlOI4CI

I 12 FFeeding inhibition by tested solution

EDN -INIITO -MPR- OF LI

Q M3

RSH EYES-shy- ( 595

- 16 -

The offf icacy of the Ageaia 6p in crop protection was tested

in t h0 f ar( r plots

EIHld I t 1 111 13

e 10t 19H71 ecember

Place Farmer plots at Han Mae Tae Amphur Sun-Sai Chiang Mai

ro_p Cauliflowers 45 days after seedling transfer

Nubur of larva S MuPims eggs were attached to the cauliflowers

leavcs (Ill 14) After 7 days larvae hatched and grew to

third instar (Ill 15) at which Lime the number of larvae

was counted prior o spraying

Treatrents T l sprayed with control solution 005 Triton in aqueous

sol ut ion

12 sprayed with an tueous suspension containing 8aof

methanolic extract-hentonite (13) rixture and 005 Triton

T3 sprayed with 101 fresh leaf aqueous etract with 005

A i ton

14 sprayed with 10 dry leaf aqueous extract with 005

A1riton

Observation - count aumber rf larvae prior to spraying 12 24 and 48

hours after spraying

Pecsu I - um arized in Ill 16

17shy

11 13

Cauliflowers plots

used in Field Test I

Ill 14 ALtaching S Uulta eggs to cauliflowers leaves

- 18 shy

11 15 - Third instar larvae from the

attached eggs

II 16 - NUR OF INSECT AFER TREATMENT WITH AGLAIA SP

t

7-iV-

ur j$N

ETH

Z

A

H u4

A

CONTROL

A D S O R B E D O N

12

WITHl LEAF MLIIANOI EXTRACT

ADSORBED ON BENTONITE

N-ET

21 Hour

WITHI FRESHi LEAF

NETiIANO EXTRACT

X

48

WITII DRY LEAF

METHtANOL EXTRACT

- 20 -

Field Test 2

Dte 21 February 1988

P1ace Farrrer plot Leside road leading to Wat Sart Luang Chiang Mal

(rop PrBccor c i oI days aft r seedl ing tran sfer

2w _r ( relicatin - 3 rplications of 1 x 3 m plot

Ir 7et T1 prayed with control solution 005Triton in aqueous

solut Ion

T2sprayed with 101 dry leaf aqueous extract with 0057

Triton

T3 sprayed with 10 dry twig aqueous cxtract with 005

1riton

Results summarized in Ill 17 and Ill 18

Ill 17 - NUMBER OF SECT AFTER TREATIMENT W1H AGLAIA SP

I--

II

DAY

CONTROL WITH DRY LEAF WITH DRY TWIG

METHANOL EXTRACI METHANOL EXTRACT

ISI 8 - -LEAF DAAGE AFTER A 7LA i S

3-2i 4

-~~~ ~ ~~~ I-

14 t N

N)

CAA

CONT RO0L t-LTII DRY L AF wl -tiDRY T WG 11EITHANOL EXTRACT 1ITHANOL EXTRACT

- 23 -

Although some degree of efficacy of Agfaia 6p cxtract in

crop prottect on was observed accurate results could not be cheived

dup to the diminishing numbers of larvae both in tie treated and control

experiment

Another uxperiment was then carried out under the Ltter

ccmtr-)Id cond t ions

late 30 March 1988

plac Pon-harvest Centre Chiang Mal University

Sra-l Broccol (25 days after seedling transfer) in clay pot

urrrunded by 1 inch of water in clay tray to prevent the

l rva from escapirng

Nu7yor of I rva 0pot

Pepi arlana -replications of 10 pots

r -r_t II sprayed with control solution 005o Triton in

aqueous solution

2 sprayed with an aqueous emulsion of 2i leaf methanolic

etract and 00O Iriton

T3 prayed with an aqueous emulsion of 2 twig methanolic

extract and 0057 Iriton

Results sumn aried in Ill 19 20 21 22 23

24 -

Ill 19- Compariscn of leaves damage 4 days after

treatment with 27 leaf methanolic extract

iMd Con tro I

Ill 20 Corrp-ison of leaf dami ge 5 days

after treatment with 24 ]eiif

metharnaoIc extract anI conitrol

- 25 shy

1121 - CoPa r I Son of leaf darrage 5 (lays

after trmarment wi th 2 leaf

methanle] ic extrLct (T 2) 2 twig

netha-nclIc extract (T 3 ) and

control (

ATERATM ITH AGLAIA Sp

a a

CONTROL WITh LEAF METHANOL WITH TWIG METHANOL EXTRACT

EXTRACT

i 23 LEA DAA P G- F A - AGLAIA

Uii

-I-I

1 4

ct cl

CONTROL tjITH LEAF METHAINOL

EXTRACTEXTRACT

WITH TI IG M shy THAN O

- 28 shy

RRIEAwaO PLAN

It can be ccn Iuded from Ihe ( iermenLs on Agtaia sp

that thre exI nt a pa qihilIty o usin this plant as an antifeedant

for Srdopten Ulna in agriculture IPlans have been node for the

I 1l1itu i IL

1) Furdther asSess other irrnu latiorn of Agaa 6p in the

field

2) Compare the activity of scopoletin which is the active

antifeedant in thi plant with other analogues

Toward the end of this reporting period we had reassesed

the insecticidol activitv of the plants which had been pieviously

studied and recorded in our earlior report with various forul ations

Pr arable roult r obtained from the prolictinary studies

0 finl period of this project will then be also spent in evaluating

their Tplical ility ira the fields

IlFFpp[ cFE

I Sai Tikwattanit Mc Thesis Kasetsart University(1977)

2Vciha Patuoumotat lSc TIhILis aseLtL rt University

Si172)

5 Rtnolh I a I I h1 Ich~z br t~nt XIAIpp (195f1) 1I209

L an a I 1 I( I) _i 109C

7 thinbaton al LJ Ec-ktt JkrCtem Soc (1912) 94 825d

C i 1 2 a nd Ling JCS

- 29 shy

9 MJBHqluy 2Cruubju 1J lam andc LAWhj t iio JCSChumI

(012 (i12) 1

IU iil 12 ~cit I ~ J03 lnuut Ilivio ( Ijibt) 14 106 3

11 V--1 lni I1~~u Ji)141 L)ad4 -i

1 Al~Kuai NMzkhhi ACuizwk iiI sjuriai Ayr hiil Chem (073) 1y(09

3 K Wda Y Li ato aind FKmnaat Acjr Iiol ChUri 0) (9 34 941

14 EWaija Y Lnuotu and K151Maiio Ag~r ia l Chem (1970) 14 94b

IKatc andu Yjluzaka ta Tet rahedron Le tter (19 74)

17 i v i v a n a d9

(1175) 2 197S

19 KLO2) 1 Miu-a anO C 1iako2j hi 3 CLrCcd(97 6701

20 Iamp Lbo LY-0I KNaLkaishi and ACiiapya JCS

2 1 I ibo SNpjgti Y- Mi ura Nakanislii and AChay

22 Kul~ 1 -4I ( 1 Pet tLe I 1ijwicz and~t~nrih

J C L Cnc1 Coo ) 10 1

23 1 F aboampi F Ha an i sh i T I 1 soh and CillWKa k aW TKuuta

24 7 Iedil Hout- Plan~rt CCC 2 C t-dice to Io it A C S Sympos i Utn

Meriv Uc2 Ameiricajn ChenicoI a00201 gt4 WaiinqLyofl DC (1977)

1 73

PAeIini Plaiin~t JtIC1tacc to l(2tsi CS Stpi r

Series~ U inri can Chemical SOcact washington DC (1977) 374

0

- 30 shy

26 Il Slurkil1 A Dictionary of the Economic Products of the

Hal ay enin-iula 2 VolMs Minitry of Agriculture and Cooprratives

huj ] Lupuira L-urpir (11)(6)

27 H Grair g t i]i I]ant Spc ie Prportedl y Possesing

I(rt-Control Propert ji-An IICUll )atabjaIe Vesource Systems

1nntjtitt irt-~e~ -tCrnter Hn)roluilu (1985)

23 P1 Tuntiwrwh tt ikurl -t al ht chremjftry (1981 )20 1164

29 T Imatayakul -t 11 A t Chrem (1979) 2 71

30 H HitnlI nll j Yrcwoto) Na tura llv Occurring Inecticides ed

7aconon H ann] Croby P( Marcel Dekker New York (1971) 62

31 W Itwers tatural Productf for Innovative Pest Management

ed Whitehead D L and flowers WS Pergqanon Press Oxford

(1903) 52

- 10 shy

111 5 Vigia kad ata 6 days after treatlrent With

crude methanolic extract (treated-coNtrol

tr ated-r-ntrol )

A smai 1 scale field test was then carried out with this

fcrrault ir 150 Vigna adiata p] ants were gron in cement pots

Ili ) After 30 day 300 third intar Iarvv of S -UJia were

l ft t r f It 1IeI i ri each pt (Ill 7) 1he plants in 4 pots

r ra ith If 1- 1d colution (Ill 8) A ii control another

p - r o raved vith n ITeuca (JUtiOn containini the same

cn ctitr a (f etvionit f l Jrilor A crr d diiference in the

v-- - I f din i wi (l 4r it (II 4 I11 )() at 24 hour

Vi a tI oa Ii-t ion of the arei of leaf dama fge and number counts of

r o riti ii a i tit t 4 and Z hrurs after spraying

qiirng tie ft l i++ini or t ul a shy

- 11 -

Calculation of area of leaf damage by S UIuka

leaf = 05) c7 of damage a(5 x + b (5 x 438)(5 x 018)

5x n

= a(25) 119) + c (09) x 100

a -- no of leaves which were d aped (r e than 507deg

b = no of leaves which were da ged ire than 25

c = no of leaves which were damiged maure than 10

(or no of leave coMtinued -pdud spots)with wei

d = no of leaven which wvr less th1r 1A damaged

(or no of lav wIth discontinued dtiraed spots)

a = no of leves which were not damaped

5 approx-- area of 4 cm 2 in which degree of damage was

est i ma ted

n = no of leaves randomly selected for checking in each

treatment

Similar test was also carried out with an aqueous extract

of 10 (ww) fresh leavo and h05 Ariton

The results of both tests were shown in 111 11 and 111 12

- 12 shy

11l 6 - Vigna adiala in cement iot 30 days old

S tua ere left to feed on Vigna kadcta lae

- 13 shy

Spraying With Lutet(I Uon

4shy

iii - Vi-a hadiata 24 hrs after spraying ith

Contro ol ion

10

- 14 shy

--J V ~gtwdiata 24 hr- afteir spraying with tested olution

- 15 -

Ill 1 - Comparison cI feedi ng a reai In

tested and control experiernt

i LE VEI I

0

HlOI4CI

I 12 FFeeding inhibition by tested solution

EDN -INIITO -MPR- OF LI

Q M3

RSH EYES-shy- ( 595

- 16 -

The offf icacy of the Ageaia 6p in crop protection was tested

in t h0 f ar( r plots

EIHld I t 1 111 13

e 10t 19H71 ecember

Place Farmer plots at Han Mae Tae Amphur Sun-Sai Chiang Mai

ro_p Cauliflowers 45 days after seedling transfer

Nubur of larva S MuPims eggs were attached to the cauliflowers

leavcs (Ill 14) After 7 days larvae hatched and grew to

third instar (Ill 15) at which Lime the number of larvae

was counted prior o spraying

Treatrents T l sprayed with control solution 005 Triton in aqueous

sol ut ion

12 sprayed with an tueous suspension containing 8aof

methanolic extract-hentonite (13) rixture and 005 Triton

T3 sprayed with 101 fresh leaf aqueous etract with 005

A i ton

14 sprayed with 10 dry leaf aqueous extract with 005

A1riton

Observation - count aumber rf larvae prior to spraying 12 24 and 48

hours after spraying

Pecsu I - um arized in Ill 16

17shy

11 13

Cauliflowers plots

used in Field Test I

Ill 14 ALtaching S Uulta eggs to cauliflowers leaves

- 18 shy

11 15 - Third instar larvae from the

attached eggs

II 16 - NUR OF INSECT AFER TREATMENT WITH AGLAIA SP

t

7-iV-

ur j$N

ETH

Z

A

H u4

A

CONTROL

A D S O R B E D O N

12

WITHl LEAF MLIIANOI EXTRACT

ADSORBED ON BENTONITE

N-ET

21 Hour

WITHI FRESHi LEAF

NETiIANO EXTRACT

X

48

WITII DRY LEAF

METHtANOL EXTRACT

- 20 -

Field Test 2

Dte 21 February 1988

P1ace Farrrer plot Leside road leading to Wat Sart Luang Chiang Mal

(rop PrBccor c i oI days aft r seedl ing tran sfer

2w _r ( relicatin - 3 rplications of 1 x 3 m plot

Ir 7et T1 prayed with control solution 005Triton in aqueous

solut Ion

T2sprayed with 101 dry leaf aqueous extract with 0057

Triton

T3 sprayed with 10 dry twig aqueous cxtract with 005

1riton

Results summarized in Ill 17 and Ill 18

Ill 17 - NUMBER OF SECT AFTER TREATIMENT W1H AGLAIA SP

I--

II

DAY

CONTROL WITH DRY LEAF WITH DRY TWIG

METHANOL EXTRACI METHANOL EXTRACT

ISI 8 - -LEAF DAAGE AFTER A 7LA i S

3-2i 4

-~~~ ~ ~~~ I-

14 t N

N)

CAA

CONT RO0L t-LTII DRY L AF wl -tiDRY T WG 11EITHANOL EXTRACT 1ITHANOL EXTRACT

- 23 -

Although some degree of efficacy of Agfaia 6p cxtract in

crop prottect on was observed accurate results could not be cheived

dup to the diminishing numbers of larvae both in tie treated and control

experiment

Another uxperiment was then carried out under the Ltter

ccmtr-)Id cond t ions

late 30 March 1988

plac Pon-harvest Centre Chiang Mal University

Sra-l Broccol (25 days after seedling transfer) in clay pot

urrrunded by 1 inch of water in clay tray to prevent the

l rva from escapirng

Nu7yor of I rva 0pot

Pepi arlana -replications of 10 pots

r -r_t II sprayed with control solution 005o Triton in

aqueous solution

2 sprayed with an aqueous emulsion of 2i leaf methanolic

etract and 00O Iriton

T3 prayed with an aqueous emulsion of 2 twig methanolic

extract and 0057 Iriton

Results sumn aried in Ill 19 20 21 22 23

24 -

Ill 19- Compariscn of leaves damage 4 days after

treatment with 27 leaf methanolic extract

iMd Con tro I

Ill 20 Corrp-ison of leaf dami ge 5 days

after treatment with 24 ]eiif

metharnaoIc extract anI conitrol

- 25 shy

1121 - CoPa r I Son of leaf darrage 5 (lays

after trmarment wi th 2 leaf

methanle] ic extrLct (T 2) 2 twig

netha-nclIc extract (T 3 ) and

control (

ATERATM ITH AGLAIA Sp

a a

CONTROL WITh LEAF METHANOL WITH TWIG METHANOL EXTRACT

EXTRACT

i 23 LEA DAA P G- F A - AGLAIA

Uii

-I-I

1 4

ct cl

CONTROL tjITH LEAF METHAINOL

EXTRACTEXTRACT

WITH TI IG M shy THAN O

- 28 shy

RRIEAwaO PLAN

It can be ccn Iuded from Ihe ( iermenLs on Agtaia sp

that thre exI nt a pa qihilIty o usin this plant as an antifeedant

for Srdopten Ulna in agriculture IPlans have been node for the

I 1l1itu i IL

1) Furdther asSess other irrnu latiorn of Agaa 6p in the

field

2) Compare the activity of scopoletin which is the active

antifeedant in thi plant with other analogues

Toward the end of this reporting period we had reassesed

the insecticidol activitv of the plants which had been pieviously

studied and recorded in our earlior report with various forul ations

Pr arable roult r obtained from the prolictinary studies

0 finl period of this project will then be also spent in evaluating

their Tplical ility ira the fields

IlFFpp[ cFE

I Sai Tikwattanit Mc Thesis Kasetsart University(1977)

2Vciha Patuoumotat lSc TIhILis aseLtL rt University

Si172)

5 Rtnolh I a I I h1 Ich~z br t~nt XIAIpp (195f1) 1I209

L an a I 1 I( I) _i 109C

7 thinbaton al LJ Ec-ktt JkrCtem Soc (1912) 94 825d

C i 1 2 a nd Ling JCS

- 29 shy

9 MJBHqluy 2Cruubju 1J lam andc LAWhj t iio JCSChumI

(012 (i12) 1

IU iil 12 ~cit I ~ J03 lnuut Ilivio ( Ijibt) 14 106 3

11 V--1 lni I1~~u Ji)141 L)ad4 -i

1 Al~Kuai NMzkhhi ACuizwk iiI sjuriai Ayr hiil Chem (073) 1y(09

3 K Wda Y Li ato aind FKmnaat Acjr Iiol ChUri 0) (9 34 941

14 EWaija Y Lnuotu and K151Maiio Ag~r ia l Chem (1970) 14 94b

IKatc andu Yjluzaka ta Tet rahedron Le tter (19 74)

17 i v i v a n a d9

(1175) 2 197S

19 KLO2) 1 Miu-a anO C 1iako2j hi 3 CLrCcd(97 6701

20 Iamp Lbo LY-0I KNaLkaishi and ACiiapya JCS

2 1 I ibo SNpjgti Y- Mi ura Nakanislii and AChay

22 Kul~ 1 -4I ( 1 Pet tLe I 1ijwicz and~t~nrih

J C L Cnc1 Coo ) 10 1

23 1 F aboampi F Ha an i sh i T I 1 soh and CillWKa k aW TKuuta

24 7 Iedil Hout- Plan~rt CCC 2 C t-dice to Io it A C S Sympos i Utn

Meriv Uc2 Ameiricajn ChenicoI a00201 gt4 WaiinqLyofl DC (1977)

1 73

PAeIini Plaiin~t JtIC1tacc to l(2tsi CS Stpi r

Series~ U inri can Chemical SOcact washington DC (1977) 374

0

- 30 shy

26 Il Slurkil1 A Dictionary of the Economic Products of the

Hal ay enin-iula 2 VolMs Minitry of Agriculture and Cooprratives

huj ] Lupuira L-urpir (11)(6)

27 H Grair g t i]i I]ant Spc ie Prportedl y Possesing

I(rt-Control Propert ji-An IICUll )atabjaIe Vesource Systems

1nntjtitt irt-~e~ -tCrnter Hn)roluilu (1985)

23 P1 Tuntiwrwh tt ikurl -t al ht chremjftry (1981 )20 1164

29 T Imatayakul -t 11 A t Chrem (1979) 2 71

30 H HitnlI nll j Yrcwoto) Na tura llv Occurring Inecticides ed

7aconon H ann] Croby P( Marcel Dekker New York (1971) 62

31 W Itwers tatural Productf for Innovative Pest Management

ed Whitehead D L and flowers WS Pergqanon Press Oxford

(1903) 52

- 11 -

Calculation of area of leaf damage by S UIuka

leaf = 05) c7 of damage a(5 x + b (5 x 438)(5 x 018)

5x n

= a(25) 119) + c (09) x 100

a -- no of leaves which were d aped (r e than 507deg

b = no of leaves which were da ged ire than 25

c = no of leaves which were damiged maure than 10

(or no of leave coMtinued -pdud spots)with wei

d = no of leaven which wvr less th1r 1A damaged

(or no of lav wIth discontinued dtiraed spots)

a = no of leves which were not damaped

5 approx-- area of 4 cm 2 in which degree of damage was

est i ma ted

n = no of leaves randomly selected for checking in each

treatment

Similar test was also carried out with an aqueous extract

of 10 (ww) fresh leavo and h05 Ariton

The results of both tests were shown in 111 11 and 111 12

- 12 shy

11l 6 - Vigna adiala in cement iot 30 days old

S tua ere left to feed on Vigna kadcta lae

- 13 shy

Spraying With Lutet(I Uon

4shy

iii - Vi-a hadiata 24 hrs after spraying ith

Contro ol ion

10

- 14 shy

--J V ~gtwdiata 24 hr- afteir spraying with tested olution

- 15 -

Ill 1 - Comparison cI feedi ng a reai In

tested and control experiernt

i LE VEI I

0

HlOI4CI

I 12 FFeeding inhibition by tested solution

EDN -INIITO -MPR- OF LI

Q M3

RSH EYES-shy- ( 595

- 16 -

The offf icacy of the Ageaia 6p in crop protection was tested

in t h0 f ar( r plots

EIHld I t 1 111 13

e 10t 19H71 ecember

Place Farmer plots at Han Mae Tae Amphur Sun-Sai Chiang Mai

ro_p Cauliflowers 45 days after seedling transfer

Nubur of larva S MuPims eggs were attached to the cauliflowers

leavcs (Ill 14) After 7 days larvae hatched and grew to

third instar (Ill 15) at which Lime the number of larvae

was counted prior o spraying

Treatrents T l sprayed with control solution 005 Triton in aqueous

sol ut ion

12 sprayed with an tueous suspension containing 8aof

methanolic extract-hentonite (13) rixture and 005 Triton

T3 sprayed with 101 fresh leaf aqueous etract with 005

A i ton

14 sprayed with 10 dry leaf aqueous extract with 005

A1riton

Observation - count aumber rf larvae prior to spraying 12 24 and 48

hours after spraying

Pecsu I - um arized in Ill 16

17shy

11 13

Cauliflowers plots

used in Field Test I

Ill 14 ALtaching S Uulta eggs to cauliflowers leaves

- 18 shy

11 15 - Third instar larvae from the

attached eggs

II 16 - NUR OF INSECT AFER TREATMENT WITH AGLAIA SP

t

7-iV-

ur j$N

ETH

Z

A

H u4

A

CONTROL

A D S O R B E D O N

12

WITHl LEAF MLIIANOI EXTRACT

ADSORBED ON BENTONITE

N-ET

21 Hour

WITHI FRESHi LEAF

NETiIANO EXTRACT

X

48

WITII DRY LEAF

METHtANOL EXTRACT

- 20 -

Field Test 2

Dte 21 February 1988

P1ace Farrrer plot Leside road leading to Wat Sart Luang Chiang Mal

(rop PrBccor c i oI days aft r seedl ing tran sfer

2w _r ( relicatin - 3 rplications of 1 x 3 m plot

Ir 7et T1 prayed with control solution 005Triton in aqueous

solut Ion

T2sprayed with 101 dry leaf aqueous extract with 0057

Triton

T3 sprayed with 10 dry twig aqueous cxtract with 005

1riton

Results summarized in Ill 17 and Ill 18

Ill 17 - NUMBER OF SECT AFTER TREATIMENT W1H AGLAIA SP

I--

II

DAY

CONTROL WITH DRY LEAF WITH DRY TWIG

METHANOL EXTRACI METHANOL EXTRACT

ISI 8 - -LEAF DAAGE AFTER A 7LA i S

3-2i 4

-~~~ ~ ~~~ I-

14 t N

N)

CAA

CONT RO0L t-LTII DRY L AF wl -tiDRY T WG 11EITHANOL EXTRACT 1ITHANOL EXTRACT

- 23 -

Although some degree of efficacy of Agfaia 6p cxtract in

crop prottect on was observed accurate results could not be cheived

dup to the diminishing numbers of larvae both in tie treated and control

experiment

Another uxperiment was then carried out under the Ltter

ccmtr-)Id cond t ions

late 30 March 1988

plac Pon-harvest Centre Chiang Mal University

Sra-l Broccol (25 days after seedling transfer) in clay pot

urrrunded by 1 inch of water in clay tray to prevent the

l rva from escapirng

Nu7yor of I rva 0pot

Pepi arlana -replications of 10 pots

r -r_t II sprayed with control solution 005o Triton in

aqueous solution

2 sprayed with an aqueous emulsion of 2i leaf methanolic

etract and 00O Iriton

T3 prayed with an aqueous emulsion of 2 twig methanolic

extract and 0057 Iriton

Results sumn aried in Ill 19 20 21 22 23

24 -

Ill 19- Compariscn of leaves damage 4 days after

treatment with 27 leaf methanolic extract

iMd Con tro I

Ill 20 Corrp-ison of leaf dami ge 5 days

after treatment with 24 ]eiif

metharnaoIc extract anI conitrol

- 25 shy

1121 - CoPa r I Son of leaf darrage 5 (lays

after trmarment wi th 2 leaf

methanle] ic extrLct (T 2) 2 twig

netha-nclIc extract (T 3 ) and

control (

ATERATM ITH AGLAIA Sp

a a

CONTROL WITh LEAF METHANOL WITH TWIG METHANOL EXTRACT

EXTRACT

i 23 LEA DAA P G- F A - AGLAIA

Uii

-I-I

1 4

ct cl

CONTROL tjITH LEAF METHAINOL

EXTRACTEXTRACT

WITH TI IG M shy THAN O

- 28 shy

RRIEAwaO PLAN

It can be ccn Iuded from Ihe ( iermenLs on Agtaia sp

that thre exI nt a pa qihilIty o usin this plant as an antifeedant

for Srdopten Ulna in agriculture IPlans have been node for the

I 1l1itu i IL

1) Furdther asSess other irrnu latiorn of Agaa 6p in the

field

2) Compare the activity of scopoletin which is the active

antifeedant in thi plant with other analogues

Toward the end of this reporting period we had reassesed

the insecticidol activitv of the plants which had been pieviously

studied and recorded in our earlior report with various forul ations

Pr arable roult r obtained from the prolictinary studies

0 finl period of this project will then be also spent in evaluating

their Tplical ility ira the fields

IlFFpp[ cFE

I Sai Tikwattanit Mc Thesis Kasetsart University(1977)

2Vciha Patuoumotat lSc TIhILis aseLtL rt University

Si172)

5 Rtnolh I a I I h1 Ich~z br t~nt XIAIpp (195f1) 1I209

L an a I 1 I( I) _i 109C

7 thinbaton al LJ Ec-ktt JkrCtem Soc (1912) 94 825d

C i 1 2 a nd Ling JCS

- 29 shy

9 MJBHqluy 2Cruubju 1J lam andc LAWhj t iio JCSChumI

(012 (i12) 1

IU iil 12 ~cit I ~ J03 lnuut Ilivio ( Ijibt) 14 106 3

11 V--1 lni I1~~u Ji)141 L)ad4 -i

1 Al~Kuai NMzkhhi ACuizwk iiI sjuriai Ayr hiil Chem (073) 1y(09

3 K Wda Y Li ato aind FKmnaat Acjr Iiol ChUri 0) (9 34 941

14 EWaija Y Lnuotu and K151Maiio Ag~r ia l Chem (1970) 14 94b

IKatc andu Yjluzaka ta Tet rahedron Le tter (19 74)

17 i v i v a n a d9

(1175) 2 197S

19 KLO2) 1 Miu-a anO C 1iako2j hi 3 CLrCcd(97 6701

20 Iamp Lbo LY-0I KNaLkaishi and ACiiapya JCS

2 1 I ibo SNpjgti Y- Mi ura Nakanislii and AChay

22 Kul~ 1 -4I ( 1 Pet tLe I 1ijwicz and~t~nrih

J C L Cnc1 Coo ) 10 1

23 1 F aboampi F Ha an i sh i T I 1 soh and CillWKa k aW TKuuta

24 7 Iedil Hout- Plan~rt CCC 2 C t-dice to Io it A C S Sympos i Utn

Meriv Uc2 Ameiricajn ChenicoI a00201 gt4 WaiinqLyofl DC (1977)

1 73

PAeIini Plaiin~t JtIC1tacc to l(2tsi CS Stpi r

Series~ U inri can Chemical SOcact washington DC (1977) 374

0

- 30 shy

26 Il Slurkil1 A Dictionary of the Economic Products of the

Hal ay enin-iula 2 VolMs Minitry of Agriculture and Cooprratives

huj ] Lupuira L-urpir (11)(6)

27 H Grair g t i]i I]ant Spc ie Prportedl y Possesing

I(rt-Control Propert ji-An IICUll )atabjaIe Vesource Systems

1nntjtitt irt-~e~ -tCrnter Hn)roluilu (1985)

23 P1 Tuntiwrwh tt ikurl -t al ht chremjftry (1981 )20 1164

29 T Imatayakul -t 11 A t Chrem (1979) 2 71

30 H HitnlI nll j Yrcwoto) Na tura llv Occurring Inecticides ed

7aconon H ann] Croby P( Marcel Dekker New York (1971) 62

31 W Itwers tatural Productf for Innovative Pest Management

ed Whitehead D L and flowers WS Pergqanon Press Oxford

(1903) 52

- 12 shy

11l 6 - Vigna adiala in cement iot 30 days old

S tua ere left to feed on Vigna kadcta lae

- 13 shy

Spraying With Lutet(I Uon

4shy

iii - Vi-a hadiata 24 hrs after spraying ith

Contro ol ion

10

- 14 shy

--J V ~gtwdiata 24 hr- afteir spraying with tested olution

- 15 -

Ill 1 - Comparison cI feedi ng a reai In

tested and control experiernt

i LE VEI I

0

HlOI4CI

I 12 FFeeding inhibition by tested solution

EDN -INIITO -MPR- OF LI

Q M3

RSH EYES-shy- ( 595

- 16 -

The offf icacy of the Ageaia 6p in crop protection was tested

in t h0 f ar( r plots

EIHld I t 1 111 13

e 10t 19H71 ecember

Place Farmer plots at Han Mae Tae Amphur Sun-Sai Chiang Mai

ro_p Cauliflowers 45 days after seedling transfer

Nubur of larva S MuPims eggs were attached to the cauliflowers

leavcs (Ill 14) After 7 days larvae hatched and grew to

third instar (Ill 15) at which Lime the number of larvae

was counted prior o spraying

Treatrents T l sprayed with control solution 005 Triton in aqueous

sol ut ion

12 sprayed with an tueous suspension containing 8aof

methanolic extract-hentonite (13) rixture and 005 Triton

T3 sprayed with 101 fresh leaf aqueous etract with 005

A i ton

14 sprayed with 10 dry leaf aqueous extract with 005

A1riton

Observation - count aumber rf larvae prior to spraying 12 24 and 48

hours after spraying

Pecsu I - um arized in Ill 16

17shy

11 13

Cauliflowers plots

used in Field Test I

Ill 14 ALtaching S Uulta eggs to cauliflowers leaves

- 18 shy

11 15 - Third instar larvae from the

attached eggs

II 16 - NUR OF INSECT AFER TREATMENT WITH AGLAIA SP

t

7-iV-

ur j$N

ETH

Z

A

H u4

A

CONTROL

A D S O R B E D O N

12

WITHl LEAF MLIIANOI EXTRACT

ADSORBED ON BENTONITE

N-ET

21 Hour

WITHI FRESHi LEAF

NETiIANO EXTRACT

X

48

WITII DRY LEAF

METHtANOL EXTRACT

- 20 -

Field Test 2

Dte 21 February 1988

P1ace Farrrer plot Leside road leading to Wat Sart Luang Chiang Mal

(rop PrBccor c i oI days aft r seedl ing tran sfer

2w _r ( relicatin - 3 rplications of 1 x 3 m plot

Ir 7et T1 prayed with control solution 005Triton in aqueous

solut Ion

T2sprayed with 101 dry leaf aqueous extract with 0057

Triton

T3 sprayed with 10 dry twig aqueous cxtract with 005

1riton

Results summarized in Ill 17 and Ill 18

Ill 17 - NUMBER OF SECT AFTER TREATIMENT W1H AGLAIA SP

I--

II

DAY

CONTROL WITH DRY LEAF WITH DRY TWIG

METHANOL EXTRACI METHANOL EXTRACT

ISI 8 - -LEAF DAAGE AFTER A 7LA i S

3-2i 4

-~~~ ~ ~~~ I-

14 t N

N)

CAA

CONT RO0L t-LTII DRY L AF wl -tiDRY T WG 11EITHANOL EXTRACT 1ITHANOL EXTRACT

- 23 -

Although some degree of efficacy of Agfaia 6p cxtract in

crop prottect on was observed accurate results could not be cheived

dup to the diminishing numbers of larvae both in tie treated and control

experiment

Another uxperiment was then carried out under the Ltter

ccmtr-)Id cond t ions

late 30 March 1988

plac Pon-harvest Centre Chiang Mal University

Sra-l Broccol (25 days after seedling transfer) in clay pot

urrrunded by 1 inch of water in clay tray to prevent the

l rva from escapirng

Nu7yor of I rva 0pot

Pepi arlana -replications of 10 pots

r -r_t II sprayed with control solution 005o Triton in

aqueous solution

2 sprayed with an aqueous emulsion of 2i leaf methanolic

etract and 00O Iriton

T3 prayed with an aqueous emulsion of 2 twig methanolic

extract and 0057 Iriton

Results sumn aried in Ill 19 20 21 22 23

24 -

Ill 19- Compariscn of leaves damage 4 days after

treatment with 27 leaf methanolic extract

iMd Con tro I

Ill 20 Corrp-ison of leaf dami ge 5 days

after treatment with 24 ]eiif

metharnaoIc extract anI conitrol

- 25 shy

1121 - CoPa r I Son of leaf darrage 5 (lays

after trmarment wi th 2 leaf

methanle] ic extrLct (T 2) 2 twig

netha-nclIc extract (T 3 ) and

control (

ATERATM ITH AGLAIA Sp

a a

CONTROL WITh LEAF METHANOL WITH TWIG METHANOL EXTRACT

EXTRACT

i 23 LEA DAA P G- F A - AGLAIA

Uii

-I-I

1 4

ct cl

CONTROL tjITH LEAF METHAINOL

EXTRACTEXTRACT

WITH TI IG M shy THAN O

- 28 shy

RRIEAwaO PLAN

It can be ccn Iuded from Ihe ( iermenLs on Agtaia sp

that thre exI nt a pa qihilIty o usin this plant as an antifeedant

for Srdopten Ulna in agriculture IPlans have been node for the

I 1l1itu i IL

1) Furdther asSess other irrnu latiorn of Agaa 6p in the

field

2) Compare the activity of scopoletin which is the active

antifeedant in thi plant with other analogues

Toward the end of this reporting period we had reassesed

the insecticidol activitv of the plants which had been pieviously

studied and recorded in our earlior report with various forul ations

Pr arable roult r obtained from the prolictinary studies

0 finl period of this project will then be also spent in evaluating

their Tplical ility ira the fields

IlFFpp[ cFE

I Sai Tikwattanit Mc Thesis Kasetsart University(1977)

2Vciha Patuoumotat lSc TIhILis aseLtL rt University

Si172)

5 Rtnolh I a I I h1 Ich~z br t~nt XIAIpp (195f1) 1I209

L an a I 1 I( I) _i 109C

7 thinbaton al LJ Ec-ktt JkrCtem Soc (1912) 94 825d

C i 1 2 a nd Ling JCS

- 29 shy

9 MJBHqluy 2Cruubju 1J lam andc LAWhj t iio JCSChumI

(012 (i12) 1

IU iil 12 ~cit I ~ J03 lnuut Ilivio ( Ijibt) 14 106 3

11 V--1 lni I1~~u Ji)141 L)ad4 -i

1 Al~Kuai NMzkhhi ACuizwk iiI sjuriai Ayr hiil Chem (073) 1y(09

3 K Wda Y Li ato aind FKmnaat Acjr Iiol ChUri 0) (9 34 941

14 EWaija Y Lnuotu and K151Maiio Ag~r ia l Chem (1970) 14 94b

IKatc andu Yjluzaka ta Tet rahedron Le tter (19 74)

17 i v i v a n a d9

(1175) 2 197S

19 KLO2) 1 Miu-a anO C 1iako2j hi 3 CLrCcd(97 6701

20 Iamp Lbo LY-0I KNaLkaishi and ACiiapya JCS

2 1 I ibo SNpjgti Y- Mi ura Nakanislii and AChay

22 Kul~ 1 -4I ( 1 Pet tLe I 1ijwicz and~t~nrih

J C L Cnc1 Coo ) 10 1

23 1 F aboampi F Ha an i sh i T I 1 soh and CillWKa k aW TKuuta

24 7 Iedil Hout- Plan~rt CCC 2 C t-dice to Io it A C S Sympos i Utn

Meriv Uc2 Ameiricajn ChenicoI a00201 gt4 WaiinqLyofl DC (1977)

1 73

PAeIini Plaiin~t JtIC1tacc to l(2tsi CS Stpi r

Series~ U inri can Chemical SOcact washington DC (1977) 374

0

- 30 shy

26 Il Slurkil1 A Dictionary of the Economic Products of the

Hal ay enin-iula 2 VolMs Minitry of Agriculture and Cooprratives

huj ] Lupuira L-urpir (11)(6)

27 H Grair g t i]i I]ant Spc ie Prportedl y Possesing

I(rt-Control Propert ji-An IICUll )atabjaIe Vesource Systems

1nntjtitt irt-~e~ -tCrnter Hn)roluilu (1985)

23 P1 Tuntiwrwh tt ikurl -t al ht chremjftry (1981 )20 1164

29 T Imatayakul -t 11 A t Chrem (1979) 2 71

30 H HitnlI nll j Yrcwoto) Na tura llv Occurring Inecticides ed

7aconon H ann] Croby P( Marcel Dekker New York (1971) 62

31 W Itwers tatural Productf for Innovative Pest Management

ed Whitehead D L and flowers WS Pergqanon Press Oxford

(1903) 52

- 13 shy

Spraying With Lutet(I Uon

4shy

iii - Vi-a hadiata 24 hrs after spraying ith

Contro ol ion

10

- 14 shy

--J V ~gtwdiata 24 hr- afteir spraying with tested olution

- 15 -

Ill 1 - Comparison cI feedi ng a reai In

tested and control experiernt

i LE VEI I

0

HlOI4CI

I 12 FFeeding inhibition by tested solution

EDN -INIITO -MPR- OF LI

Q M3

RSH EYES-shy- ( 595

- 16 -

The offf icacy of the Ageaia 6p in crop protection was tested

in t h0 f ar( r plots

EIHld I t 1 111 13

e 10t 19H71 ecember

Place Farmer plots at Han Mae Tae Amphur Sun-Sai Chiang Mai

ro_p Cauliflowers 45 days after seedling transfer

Nubur of larva S MuPims eggs were attached to the cauliflowers

leavcs (Ill 14) After 7 days larvae hatched and grew to

third instar (Ill 15) at which Lime the number of larvae

was counted prior o spraying

Treatrents T l sprayed with control solution 005 Triton in aqueous

sol ut ion

12 sprayed with an tueous suspension containing 8aof

methanolic extract-hentonite (13) rixture and 005 Triton

T3 sprayed with 101 fresh leaf aqueous etract with 005

A i ton

14 sprayed with 10 dry leaf aqueous extract with 005

A1riton

Observation - count aumber rf larvae prior to spraying 12 24 and 48

hours after spraying

Pecsu I - um arized in Ill 16

17shy

11 13

Cauliflowers plots

used in Field Test I

Ill 14 ALtaching S Uulta eggs to cauliflowers leaves

- 18 shy

11 15 - Third instar larvae from the

attached eggs

II 16 - NUR OF INSECT AFER TREATMENT WITH AGLAIA SP

t

7-iV-

ur j$N

ETH

Z

A

H u4

A

CONTROL

A D S O R B E D O N

12

WITHl LEAF MLIIANOI EXTRACT

ADSORBED ON BENTONITE

N-ET

21 Hour

WITHI FRESHi LEAF

NETiIANO EXTRACT

X

48

WITII DRY LEAF

METHtANOL EXTRACT

- 20 -

Field Test 2

Dte 21 February 1988

P1ace Farrrer plot Leside road leading to Wat Sart Luang Chiang Mal

(rop PrBccor c i oI days aft r seedl ing tran sfer

2w _r ( relicatin - 3 rplications of 1 x 3 m plot

Ir 7et T1 prayed with control solution 005Triton in aqueous

solut Ion

T2sprayed with 101 dry leaf aqueous extract with 0057

Triton

T3 sprayed with 10 dry twig aqueous cxtract with 005

1riton

Results summarized in Ill 17 and Ill 18

Ill 17 - NUMBER OF SECT AFTER TREATIMENT W1H AGLAIA SP

I--

II

DAY

CONTROL WITH DRY LEAF WITH DRY TWIG

METHANOL EXTRACI METHANOL EXTRACT

ISI 8 - -LEAF DAAGE AFTER A 7LA i S

3-2i 4

-~~~ ~ ~~~ I-

14 t N

N)

CAA

CONT RO0L t-LTII DRY L AF wl -tiDRY T WG 11EITHANOL EXTRACT 1ITHANOL EXTRACT

- 23 -

Although some degree of efficacy of Agfaia 6p cxtract in

crop prottect on was observed accurate results could not be cheived

dup to the diminishing numbers of larvae both in tie treated and control

experiment

Another uxperiment was then carried out under the Ltter

ccmtr-)Id cond t ions

late 30 March 1988

plac Pon-harvest Centre Chiang Mal University

Sra-l Broccol (25 days after seedling transfer) in clay pot

urrrunded by 1 inch of water in clay tray to prevent the

l rva from escapirng

Nu7yor of I rva 0pot

Pepi arlana -replications of 10 pots

r -r_t II sprayed with control solution 005o Triton in

aqueous solution

2 sprayed with an aqueous emulsion of 2i leaf methanolic

etract and 00O Iriton

T3 prayed with an aqueous emulsion of 2 twig methanolic

extract and 0057 Iriton

Results sumn aried in Ill 19 20 21 22 23

24 -

Ill 19- Compariscn of leaves damage 4 days after

treatment with 27 leaf methanolic extract

iMd Con tro I

Ill 20 Corrp-ison of leaf dami ge 5 days

after treatment with 24 ]eiif

metharnaoIc extract anI conitrol

- 25 shy

1121 - CoPa r I Son of leaf darrage 5 (lays

after trmarment wi th 2 leaf

methanle] ic extrLct (T 2) 2 twig

netha-nclIc extract (T 3 ) and

control (

ATERATM ITH AGLAIA Sp

a a

CONTROL WITh LEAF METHANOL WITH TWIG METHANOL EXTRACT

EXTRACT

i 23 LEA DAA P G- F A - AGLAIA

Uii

-I-I

1 4

ct cl

CONTROL tjITH LEAF METHAINOL

EXTRACTEXTRACT

WITH TI IG M shy THAN O

- 28 shy

RRIEAwaO PLAN

It can be ccn Iuded from Ihe ( iermenLs on Agtaia sp

that thre exI nt a pa qihilIty o usin this plant as an antifeedant

for Srdopten Ulna in agriculture IPlans have been node for the

I 1l1itu i IL

1) Furdther asSess other irrnu latiorn of Agaa 6p in the

field

2) Compare the activity of scopoletin which is the active

antifeedant in thi plant with other analogues

Toward the end of this reporting period we had reassesed

the insecticidol activitv of the plants which had been pieviously

studied and recorded in our earlior report with various forul ations

Pr arable roult r obtained from the prolictinary studies

0 finl period of this project will then be also spent in evaluating

their Tplical ility ira the fields

IlFFpp[ cFE

I Sai Tikwattanit Mc Thesis Kasetsart University(1977)

2Vciha Patuoumotat lSc TIhILis aseLtL rt University

Si172)

5 Rtnolh I a I I h1 Ich~z br t~nt XIAIpp (195f1) 1I209

L an a I 1 I( I) _i 109C

7 thinbaton al LJ Ec-ktt JkrCtem Soc (1912) 94 825d

C i 1 2 a nd Ling JCS

- 29 shy

9 MJBHqluy 2Cruubju 1J lam andc LAWhj t iio JCSChumI

(012 (i12) 1

IU iil 12 ~cit I ~ J03 lnuut Ilivio ( Ijibt) 14 106 3

11 V--1 lni I1~~u Ji)141 L)ad4 -i

1 Al~Kuai NMzkhhi ACuizwk iiI sjuriai Ayr hiil Chem (073) 1y(09

3 K Wda Y Li ato aind FKmnaat Acjr Iiol ChUri 0) (9 34 941

14 EWaija Y Lnuotu and K151Maiio Ag~r ia l Chem (1970) 14 94b

IKatc andu Yjluzaka ta Tet rahedron Le tter (19 74)

17 i v i v a n a d9

(1175) 2 197S

19 KLO2) 1 Miu-a anO C 1iako2j hi 3 CLrCcd(97 6701

20 Iamp Lbo LY-0I KNaLkaishi and ACiiapya JCS

2 1 I ibo SNpjgti Y- Mi ura Nakanislii and AChay

22 Kul~ 1 -4I ( 1 Pet tLe I 1ijwicz and~t~nrih

J C L Cnc1 Coo ) 10 1

23 1 F aboampi F Ha an i sh i T I 1 soh and CillWKa k aW TKuuta

24 7 Iedil Hout- Plan~rt CCC 2 C t-dice to Io it A C S Sympos i Utn

Meriv Uc2 Ameiricajn ChenicoI a00201 gt4 WaiinqLyofl DC (1977)

1 73

PAeIini Plaiin~t JtIC1tacc to l(2tsi CS Stpi r

Series~ U inri can Chemical SOcact washington DC (1977) 374

0

- 30 shy

26 Il Slurkil1 A Dictionary of the Economic Products of the

Hal ay enin-iula 2 VolMs Minitry of Agriculture and Cooprratives

huj ] Lupuira L-urpir (11)(6)

27 H Grair g t i]i I]ant Spc ie Prportedl y Possesing

I(rt-Control Propert ji-An IICUll )atabjaIe Vesource Systems

1nntjtitt irt-~e~ -tCrnter Hn)roluilu (1985)

23 P1 Tuntiwrwh tt ikurl -t al ht chremjftry (1981 )20 1164

29 T Imatayakul -t 11 A t Chrem (1979) 2 71

30 H HitnlI nll j Yrcwoto) Na tura llv Occurring Inecticides ed

7aconon H ann] Croby P( Marcel Dekker New York (1971) 62

31 W Itwers tatural Productf for Innovative Pest Management

ed Whitehead D L and flowers WS Pergqanon Press Oxford

(1903) 52

10

- 14 shy

--J V ~gtwdiata 24 hr- afteir spraying with tested olution

- 15 -

Ill 1 - Comparison cI feedi ng a reai In

tested and control experiernt

i LE VEI I

0

HlOI4CI

I 12 FFeeding inhibition by tested solution

EDN -INIITO -MPR- OF LI

Q M3

RSH EYES-shy- ( 595

- 16 -

The offf icacy of the Ageaia 6p in crop protection was tested

in t h0 f ar( r plots

EIHld I t 1 111 13

e 10t 19H71 ecember

Place Farmer plots at Han Mae Tae Amphur Sun-Sai Chiang Mai

ro_p Cauliflowers 45 days after seedling transfer

Nubur of larva S MuPims eggs were attached to the cauliflowers

leavcs (Ill 14) After 7 days larvae hatched and grew to

third instar (Ill 15) at which Lime the number of larvae

was counted prior o spraying

Treatrents T l sprayed with control solution 005 Triton in aqueous

sol ut ion

12 sprayed with an tueous suspension containing 8aof

methanolic extract-hentonite (13) rixture and 005 Triton

T3 sprayed with 101 fresh leaf aqueous etract with 005

A i ton

14 sprayed with 10 dry leaf aqueous extract with 005

A1riton

Observation - count aumber rf larvae prior to spraying 12 24 and 48

hours after spraying

Pecsu I - um arized in Ill 16

17shy

11 13

Cauliflowers plots

used in Field Test I

Ill 14 ALtaching S Uulta eggs to cauliflowers leaves

- 18 shy

11 15 - Third instar larvae from the

attached eggs

II 16 - NUR OF INSECT AFER TREATMENT WITH AGLAIA SP

t

7-iV-

ur j$N

ETH

Z

A

H u4

A

CONTROL

A D S O R B E D O N

12

WITHl LEAF MLIIANOI EXTRACT

ADSORBED ON BENTONITE

N-ET

21 Hour

WITHI FRESHi LEAF

NETiIANO EXTRACT

X

48

WITII DRY LEAF

METHtANOL EXTRACT

- 20 -

Field Test 2

Dte 21 February 1988

P1ace Farrrer plot Leside road leading to Wat Sart Luang Chiang Mal

(rop PrBccor c i oI days aft r seedl ing tran sfer

2w _r ( relicatin - 3 rplications of 1 x 3 m plot

Ir 7et T1 prayed with control solution 005Triton in aqueous

solut Ion

T2sprayed with 101 dry leaf aqueous extract with 0057

Triton

T3 sprayed with 10 dry twig aqueous cxtract with 005

1riton

Results summarized in Ill 17 and Ill 18

Ill 17 - NUMBER OF SECT AFTER TREATIMENT W1H AGLAIA SP

I--

II

DAY

CONTROL WITH DRY LEAF WITH DRY TWIG

METHANOL EXTRACI METHANOL EXTRACT

ISI 8 - -LEAF DAAGE AFTER A 7LA i S

3-2i 4

-~~~ ~ ~~~ I-

14 t N

N)

CAA

CONT RO0L t-LTII DRY L AF wl -tiDRY T WG 11EITHANOL EXTRACT 1ITHANOL EXTRACT

- 23 -

Although some degree of efficacy of Agfaia 6p cxtract in

crop prottect on was observed accurate results could not be cheived

dup to the diminishing numbers of larvae both in tie treated and control

experiment

Another uxperiment was then carried out under the Ltter

ccmtr-)Id cond t ions

late 30 March 1988

plac Pon-harvest Centre Chiang Mal University

Sra-l Broccol (25 days after seedling transfer) in clay pot

urrrunded by 1 inch of water in clay tray to prevent the

l rva from escapirng

Nu7yor of I rva 0pot

Pepi arlana -replications of 10 pots

r -r_t II sprayed with control solution 005o Triton in

aqueous solution

2 sprayed with an aqueous emulsion of 2i leaf methanolic

etract and 00O Iriton

T3 prayed with an aqueous emulsion of 2 twig methanolic

extract and 0057 Iriton

Results sumn aried in Ill 19 20 21 22 23

24 -

Ill 19- Compariscn of leaves damage 4 days after

treatment with 27 leaf methanolic extract

iMd Con tro I

Ill 20 Corrp-ison of leaf dami ge 5 days

after treatment with 24 ]eiif

metharnaoIc extract anI conitrol

- 25 shy

1121 - CoPa r I Son of leaf darrage 5 (lays

after trmarment wi th 2 leaf

methanle] ic extrLct (T 2) 2 twig

netha-nclIc extract (T 3 ) and

control (

ATERATM ITH AGLAIA Sp

a a

CONTROL WITh LEAF METHANOL WITH TWIG METHANOL EXTRACT

EXTRACT

i 23 LEA DAA P G- F A - AGLAIA

Uii

-I-I

1 4

ct cl

CONTROL tjITH LEAF METHAINOL

EXTRACTEXTRACT

WITH TI IG M shy THAN O

- 28 shy

RRIEAwaO PLAN

It can be ccn Iuded from Ihe ( iermenLs on Agtaia sp

that thre exI nt a pa qihilIty o usin this plant as an antifeedant

for Srdopten Ulna in agriculture IPlans have been node for the

I 1l1itu i IL

1) Furdther asSess other irrnu latiorn of Agaa 6p in the

field

2) Compare the activity of scopoletin which is the active

antifeedant in thi plant with other analogues

Toward the end of this reporting period we had reassesed

the insecticidol activitv of the plants which had been pieviously

studied and recorded in our earlior report with various forul ations

Pr arable roult r obtained from the prolictinary studies

0 finl period of this project will then be also spent in evaluating

their Tplical ility ira the fields

IlFFpp[ cFE

I Sai Tikwattanit Mc Thesis Kasetsart University(1977)

2Vciha Patuoumotat lSc TIhILis aseLtL rt University

Si172)

5 Rtnolh I a I I h1 Ich~z br t~nt XIAIpp (195f1) 1I209

L an a I 1 I( I) _i 109C

7 thinbaton al LJ Ec-ktt JkrCtem Soc (1912) 94 825d

C i 1 2 a nd Ling JCS

- 29 shy

9 MJBHqluy 2Cruubju 1J lam andc LAWhj t iio JCSChumI

(012 (i12) 1

IU iil 12 ~cit I ~ J03 lnuut Ilivio ( Ijibt) 14 106 3

11 V--1 lni I1~~u Ji)141 L)ad4 -i

1 Al~Kuai NMzkhhi ACuizwk iiI sjuriai Ayr hiil Chem (073) 1y(09

3 K Wda Y Li ato aind FKmnaat Acjr Iiol ChUri 0) (9 34 941

14 EWaija Y Lnuotu and K151Maiio Ag~r ia l Chem (1970) 14 94b

IKatc andu Yjluzaka ta Tet rahedron Le tter (19 74)

17 i v i v a n a d9

(1175) 2 197S

19 KLO2) 1 Miu-a anO C 1iako2j hi 3 CLrCcd(97 6701

20 Iamp Lbo LY-0I KNaLkaishi and ACiiapya JCS

2 1 I ibo SNpjgti Y- Mi ura Nakanislii and AChay

22 Kul~ 1 -4I ( 1 Pet tLe I 1ijwicz and~t~nrih

J C L Cnc1 Coo ) 10 1

23 1 F aboampi F Ha an i sh i T I 1 soh and CillWKa k aW TKuuta

24 7 Iedil Hout- Plan~rt CCC 2 C t-dice to Io it A C S Sympos i Utn

Meriv Uc2 Ameiricajn ChenicoI a00201 gt4 WaiinqLyofl DC (1977)

1 73

PAeIini Plaiin~t JtIC1tacc to l(2tsi CS Stpi r

Series~ U inri can Chemical SOcact washington DC (1977) 374

0

- 30 shy

26 Il Slurkil1 A Dictionary of the Economic Products of the

Hal ay enin-iula 2 VolMs Minitry of Agriculture and Cooprratives

huj ] Lupuira L-urpir (11)(6)

27 H Grair g t i]i I]ant Spc ie Prportedl y Possesing

I(rt-Control Propert ji-An IICUll )atabjaIe Vesource Systems

1nntjtitt irt-~e~ -tCrnter Hn)roluilu (1985)

23 P1 Tuntiwrwh tt ikurl -t al ht chremjftry (1981 )20 1164

29 T Imatayakul -t 11 A t Chrem (1979) 2 71

30 H HitnlI nll j Yrcwoto) Na tura llv Occurring Inecticides ed

7aconon H ann] Croby P( Marcel Dekker New York (1971) 62

31 W Itwers tatural Productf for Innovative Pest Management

ed Whitehead D L and flowers WS Pergqanon Press Oxford

(1903) 52

- 15 -

Ill 1 - Comparison cI feedi ng a reai In

tested and control experiernt

i LE VEI I

0

HlOI4CI

I 12 FFeeding inhibition by tested solution

EDN -INIITO -MPR- OF LI

Q M3

RSH EYES-shy- ( 595

- 16 -

The offf icacy of the Ageaia 6p in crop protection was tested

in t h0 f ar( r plots

EIHld I t 1 111 13

e 10t 19H71 ecember

Place Farmer plots at Han Mae Tae Amphur Sun-Sai Chiang Mai

ro_p Cauliflowers 45 days after seedling transfer

Nubur of larva S MuPims eggs were attached to the cauliflowers

leavcs (Ill 14) After 7 days larvae hatched and grew to

third instar (Ill 15) at which Lime the number of larvae

was counted prior o spraying

Treatrents T l sprayed with control solution 005 Triton in aqueous

sol ut ion

12 sprayed with an tueous suspension containing 8aof

methanolic extract-hentonite (13) rixture and 005 Triton

T3 sprayed with 101 fresh leaf aqueous etract with 005

A i ton

14 sprayed with 10 dry leaf aqueous extract with 005

A1riton

Observation - count aumber rf larvae prior to spraying 12 24 and 48

hours after spraying

Pecsu I - um arized in Ill 16

17shy

11 13

Cauliflowers plots

used in Field Test I

Ill 14 ALtaching S Uulta eggs to cauliflowers leaves

- 18 shy

11 15 - Third instar larvae from the

attached eggs

II 16 - NUR OF INSECT AFER TREATMENT WITH AGLAIA SP

t

7-iV-

ur j$N

ETH

Z

A

H u4

A

CONTROL

A D S O R B E D O N

12

WITHl LEAF MLIIANOI EXTRACT

ADSORBED ON BENTONITE

N-ET

21 Hour

WITHI FRESHi LEAF

NETiIANO EXTRACT

X

48

WITII DRY LEAF

METHtANOL EXTRACT

- 20 -

Field Test 2

Dte 21 February 1988

P1ace Farrrer plot Leside road leading to Wat Sart Luang Chiang Mal

(rop PrBccor c i oI days aft r seedl ing tran sfer

2w _r ( relicatin - 3 rplications of 1 x 3 m plot

Ir 7et T1 prayed with control solution 005Triton in aqueous

solut Ion

T2sprayed with 101 dry leaf aqueous extract with 0057

Triton

T3 sprayed with 10 dry twig aqueous cxtract with 005

1riton

Results summarized in Ill 17 and Ill 18

Ill 17 - NUMBER OF SECT AFTER TREATIMENT W1H AGLAIA SP

I--

II

DAY

CONTROL WITH DRY LEAF WITH DRY TWIG

METHANOL EXTRACI METHANOL EXTRACT

ISI 8 - -LEAF DAAGE AFTER A 7LA i S

3-2i 4

-~~~ ~ ~~~ I-

14 t N

N)

CAA

CONT RO0L t-LTII DRY L AF wl -tiDRY T WG 11EITHANOL EXTRACT 1ITHANOL EXTRACT

- 23 -

Although some degree of efficacy of Agfaia 6p cxtract in

crop prottect on was observed accurate results could not be cheived

dup to the diminishing numbers of larvae both in tie treated and control

experiment

Another uxperiment was then carried out under the Ltter

ccmtr-)Id cond t ions

late 30 March 1988

plac Pon-harvest Centre Chiang Mal University

Sra-l Broccol (25 days after seedling transfer) in clay pot

urrrunded by 1 inch of water in clay tray to prevent the

l rva from escapirng

Nu7yor of I rva 0pot

Pepi arlana -replications of 10 pots

r -r_t II sprayed with control solution 005o Triton in

aqueous solution

2 sprayed with an aqueous emulsion of 2i leaf methanolic

etract and 00O Iriton

T3 prayed with an aqueous emulsion of 2 twig methanolic

extract and 0057 Iriton

Results sumn aried in Ill 19 20 21 22 23

24 -

Ill 19- Compariscn of leaves damage 4 days after

treatment with 27 leaf methanolic extract

iMd Con tro I

Ill 20 Corrp-ison of leaf dami ge 5 days

after treatment with 24 ]eiif

metharnaoIc extract anI conitrol

- 25 shy

1121 - CoPa r I Son of leaf darrage 5 (lays

after trmarment wi th 2 leaf

methanle] ic extrLct (T 2) 2 twig

netha-nclIc extract (T 3 ) and

control (

ATERATM ITH AGLAIA Sp

a a

CONTROL WITh LEAF METHANOL WITH TWIG METHANOL EXTRACT

EXTRACT

i 23 LEA DAA P G- F A - AGLAIA

Uii

-I-I

1 4

ct cl

CONTROL tjITH LEAF METHAINOL

EXTRACTEXTRACT

WITH TI IG M shy THAN O

- 28 shy

RRIEAwaO PLAN

It can be ccn Iuded from Ihe ( iermenLs on Agtaia sp

that thre exI nt a pa qihilIty o usin this plant as an antifeedant

for Srdopten Ulna in agriculture IPlans have been node for the

I 1l1itu i IL

1) Furdther asSess other irrnu latiorn of Agaa 6p in the

field

2) Compare the activity of scopoletin which is the active

antifeedant in thi plant with other analogues

Toward the end of this reporting period we had reassesed

the insecticidol activitv of the plants which had been pieviously

studied and recorded in our earlior report with various forul ations

Pr arable roult r obtained from the prolictinary studies

0 finl period of this project will then be also spent in evaluating

their Tplical ility ira the fields

IlFFpp[ cFE

I Sai Tikwattanit Mc Thesis Kasetsart University(1977)

2Vciha Patuoumotat lSc TIhILis aseLtL rt University

Si172)

5 Rtnolh I a I I h1 Ich~z br t~nt XIAIpp (195f1) 1I209

L an a I 1 I( I) _i 109C

7 thinbaton al LJ Ec-ktt JkrCtem Soc (1912) 94 825d

C i 1 2 a nd Ling JCS

- 29 shy

9 MJBHqluy 2Cruubju 1J lam andc LAWhj t iio JCSChumI

(012 (i12) 1

IU iil 12 ~cit I ~ J03 lnuut Ilivio ( Ijibt) 14 106 3

11 V--1 lni I1~~u Ji)141 L)ad4 -i

1 Al~Kuai NMzkhhi ACuizwk iiI sjuriai Ayr hiil Chem (073) 1y(09

3 K Wda Y Li ato aind FKmnaat Acjr Iiol ChUri 0) (9 34 941

14 EWaija Y Lnuotu and K151Maiio Ag~r ia l Chem (1970) 14 94b

IKatc andu Yjluzaka ta Tet rahedron Le tter (19 74)

17 i v i v a n a d9

(1175) 2 197S

19 KLO2) 1 Miu-a anO C 1iako2j hi 3 CLrCcd(97 6701

20 Iamp Lbo LY-0I KNaLkaishi and ACiiapya JCS

2 1 I ibo SNpjgti Y- Mi ura Nakanislii and AChay

22 Kul~ 1 -4I ( 1 Pet tLe I 1ijwicz and~t~nrih

J C L Cnc1 Coo ) 10 1

23 1 F aboampi F Ha an i sh i T I 1 soh and CillWKa k aW TKuuta

24 7 Iedil Hout- Plan~rt CCC 2 C t-dice to Io it A C S Sympos i Utn

Meriv Uc2 Ameiricajn ChenicoI a00201 gt4 WaiinqLyofl DC (1977)

1 73

PAeIini Plaiin~t JtIC1tacc to l(2tsi CS Stpi r

Series~ U inri can Chemical SOcact washington DC (1977) 374

0

- 30 shy

26 Il Slurkil1 A Dictionary of the Economic Products of the

Hal ay enin-iula 2 VolMs Minitry of Agriculture and Cooprratives

huj ] Lupuira L-urpir (11)(6)

27 H Grair g t i]i I]ant Spc ie Prportedl y Possesing

I(rt-Control Propert ji-An IICUll )atabjaIe Vesource Systems

1nntjtitt irt-~e~ -tCrnter Hn)roluilu (1985)

23 P1 Tuntiwrwh tt ikurl -t al ht chremjftry (1981 )20 1164

29 T Imatayakul -t 11 A t Chrem (1979) 2 71

30 H HitnlI nll j Yrcwoto) Na tura llv Occurring Inecticides ed

7aconon H ann] Croby P( Marcel Dekker New York (1971) 62

31 W Itwers tatural Productf for Innovative Pest Management

ed Whitehead D L and flowers WS Pergqanon Press Oxford

(1903) 52

- 16 -

The offf icacy of the Ageaia 6p in crop protection was tested

in t h0 f ar( r plots

EIHld I t 1 111 13

e 10t 19H71 ecember

Place Farmer plots at Han Mae Tae Amphur Sun-Sai Chiang Mai

ro_p Cauliflowers 45 days after seedling transfer

Nubur of larva S MuPims eggs were attached to the cauliflowers

leavcs (Ill 14) After 7 days larvae hatched and grew to

third instar (Ill 15) at which Lime the number of larvae

was counted prior o spraying

Treatrents T l sprayed with control solution 005 Triton in aqueous

sol ut ion

12 sprayed with an tueous suspension containing 8aof

methanolic extract-hentonite (13) rixture and 005 Triton

T3 sprayed with 101 fresh leaf aqueous etract with 005

A i ton

14 sprayed with 10 dry leaf aqueous extract with 005

A1riton

Observation - count aumber rf larvae prior to spraying 12 24 and 48

hours after spraying

Pecsu I - um arized in Ill 16

17shy

11 13

Cauliflowers plots

used in Field Test I

Ill 14 ALtaching S Uulta eggs to cauliflowers leaves

- 18 shy

11 15 - Third instar larvae from the

attached eggs

II 16 - NUR OF INSECT AFER TREATMENT WITH AGLAIA SP

t

7-iV-

ur j$N

ETH

Z

A

H u4

A

CONTROL

A D S O R B E D O N

12

WITHl LEAF MLIIANOI EXTRACT

ADSORBED ON BENTONITE

N-ET

21 Hour

WITHI FRESHi LEAF

NETiIANO EXTRACT

X

48

WITII DRY LEAF

METHtANOL EXTRACT

- 20 -

Field Test 2

Dte 21 February 1988

P1ace Farrrer plot Leside road leading to Wat Sart Luang Chiang Mal

(rop PrBccor c i oI days aft r seedl ing tran sfer

2w _r ( relicatin - 3 rplications of 1 x 3 m plot

Ir 7et T1 prayed with control solution 005Triton in aqueous

solut Ion

T2sprayed with 101 dry leaf aqueous extract with 0057

Triton

T3 sprayed with 10 dry twig aqueous cxtract with 005

1riton

Results summarized in Ill 17 and Ill 18

Ill 17 - NUMBER OF SECT AFTER TREATIMENT W1H AGLAIA SP

I--

II

DAY

CONTROL WITH DRY LEAF WITH DRY TWIG

METHANOL EXTRACI METHANOL EXTRACT

ISI 8 - -LEAF DAAGE AFTER A 7LA i S

3-2i 4

-~~~ ~ ~~~ I-

14 t N

N)

CAA

CONT RO0L t-LTII DRY L AF wl -tiDRY T WG 11EITHANOL EXTRACT 1ITHANOL EXTRACT

- 23 -

Although some degree of efficacy of Agfaia 6p cxtract in

crop prottect on was observed accurate results could not be cheived

dup to the diminishing numbers of larvae both in tie treated and control

experiment

Another uxperiment was then carried out under the Ltter

ccmtr-)Id cond t ions

late 30 March 1988

plac Pon-harvest Centre Chiang Mal University

Sra-l Broccol (25 days after seedling transfer) in clay pot

urrrunded by 1 inch of water in clay tray to prevent the

l rva from escapirng

Nu7yor of I rva 0pot

Pepi arlana -replications of 10 pots

r -r_t II sprayed with control solution 005o Triton in

aqueous solution

2 sprayed with an aqueous emulsion of 2i leaf methanolic

etract and 00O Iriton

T3 prayed with an aqueous emulsion of 2 twig methanolic

extract and 0057 Iriton

Results sumn aried in Ill 19 20 21 22 23

24 -

Ill 19- Compariscn of leaves damage 4 days after

treatment with 27 leaf methanolic extract

iMd Con tro I

Ill 20 Corrp-ison of leaf dami ge 5 days

after treatment with 24 ]eiif

metharnaoIc extract anI conitrol

- 25 shy

1121 - CoPa r I Son of leaf darrage 5 (lays

after trmarment wi th 2 leaf

methanle] ic extrLct (T 2) 2 twig

netha-nclIc extract (T 3 ) and

control (

ATERATM ITH AGLAIA Sp

a a

CONTROL WITh LEAF METHANOL WITH TWIG METHANOL EXTRACT

EXTRACT

i 23 LEA DAA P G- F A - AGLAIA

Uii

-I-I

1 4

ct cl

CONTROL tjITH LEAF METHAINOL

EXTRACTEXTRACT

WITH TI IG M shy THAN O

- 28 shy

RRIEAwaO PLAN

It can be ccn Iuded from Ihe ( iermenLs on Agtaia sp

that thre exI nt a pa qihilIty o usin this plant as an antifeedant

for Srdopten Ulna in agriculture IPlans have been node for the

I 1l1itu i IL

1) Furdther asSess other irrnu latiorn of Agaa 6p in the

field

2) Compare the activity of scopoletin which is the active

antifeedant in thi plant with other analogues

Toward the end of this reporting period we had reassesed

the insecticidol activitv of the plants which had been pieviously

studied and recorded in our earlior report with various forul ations

Pr arable roult r obtained from the prolictinary studies

0 finl period of this project will then be also spent in evaluating

their Tplical ility ira the fields

IlFFpp[ cFE

I Sai Tikwattanit Mc Thesis Kasetsart University(1977)

2Vciha Patuoumotat lSc TIhILis aseLtL rt University

Si172)

5 Rtnolh I a I I h1 Ich~z br t~nt XIAIpp (195f1) 1I209

L an a I 1 I( I) _i 109C

7 thinbaton al LJ Ec-ktt JkrCtem Soc (1912) 94 825d

C i 1 2 a nd Ling JCS

- 29 shy

9 MJBHqluy 2Cruubju 1J lam andc LAWhj t iio JCSChumI

(012 (i12) 1

IU iil 12 ~cit I ~ J03 lnuut Ilivio ( Ijibt) 14 106 3

11 V--1 lni I1~~u Ji)141 L)ad4 -i

1 Al~Kuai NMzkhhi ACuizwk iiI sjuriai Ayr hiil Chem (073) 1y(09

3 K Wda Y Li ato aind FKmnaat Acjr Iiol ChUri 0) (9 34 941

14 EWaija Y Lnuotu and K151Maiio Ag~r ia l Chem (1970) 14 94b

IKatc andu Yjluzaka ta Tet rahedron Le tter (19 74)

17 i v i v a n a d9

(1175) 2 197S

19 KLO2) 1 Miu-a anO C 1iako2j hi 3 CLrCcd(97 6701

20 Iamp Lbo LY-0I KNaLkaishi and ACiiapya JCS

2 1 I ibo SNpjgti Y- Mi ura Nakanislii and AChay

22 Kul~ 1 -4I ( 1 Pet tLe I 1ijwicz and~t~nrih

J C L Cnc1 Coo ) 10 1

23 1 F aboampi F Ha an i sh i T I 1 soh and CillWKa k aW TKuuta

24 7 Iedil Hout- Plan~rt CCC 2 C t-dice to Io it A C S Sympos i Utn

Meriv Uc2 Ameiricajn ChenicoI a00201 gt4 WaiinqLyofl DC (1977)

1 73

PAeIini Plaiin~t JtIC1tacc to l(2tsi CS Stpi r

Series~ U inri can Chemical SOcact washington DC (1977) 374

0

- 30 shy

26 Il Slurkil1 A Dictionary of the Economic Products of the

Hal ay enin-iula 2 VolMs Minitry of Agriculture and Cooprratives

huj ] Lupuira L-urpir (11)(6)

27 H Grair g t i]i I]ant Spc ie Prportedl y Possesing

I(rt-Control Propert ji-An IICUll )atabjaIe Vesource Systems

1nntjtitt irt-~e~ -tCrnter Hn)roluilu (1985)

23 P1 Tuntiwrwh tt ikurl -t al ht chremjftry (1981 )20 1164

29 T Imatayakul -t 11 A t Chrem (1979) 2 71

30 H HitnlI nll j Yrcwoto) Na tura llv Occurring Inecticides ed

7aconon H ann] Croby P( Marcel Dekker New York (1971) 62

31 W Itwers tatural Productf for Innovative Pest Management

ed Whitehead D L and flowers WS Pergqanon Press Oxford

(1903) 52

17shy

11 13

Cauliflowers plots

used in Field Test I

Ill 14 ALtaching S Uulta eggs to cauliflowers leaves

- 18 shy

11 15 - Third instar larvae from the

attached eggs

II 16 - NUR OF INSECT AFER TREATMENT WITH AGLAIA SP

t

7-iV-

ur j$N

ETH

Z

A

H u4

A

CONTROL

A D S O R B E D O N

12

WITHl LEAF MLIIANOI EXTRACT

ADSORBED ON BENTONITE

N-ET

21 Hour

WITHI FRESHi LEAF

NETiIANO EXTRACT

X

48

WITII DRY LEAF

METHtANOL EXTRACT

- 20 -

Field Test 2

Dte 21 February 1988

P1ace Farrrer plot Leside road leading to Wat Sart Luang Chiang Mal

(rop PrBccor c i oI days aft r seedl ing tran sfer

2w _r ( relicatin - 3 rplications of 1 x 3 m plot

Ir 7et T1 prayed with control solution 005Triton in aqueous

solut Ion

T2sprayed with 101 dry leaf aqueous extract with 0057

Triton

T3 sprayed with 10 dry twig aqueous cxtract with 005

1riton

Results summarized in Ill 17 and Ill 18

Ill 17 - NUMBER OF SECT AFTER TREATIMENT W1H AGLAIA SP

I--

II

DAY

CONTROL WITH DRY LEAF WITH DRY TWIG

METHANOL EXTRACI METHANOL EXTRACT

ISI 8 - -LEAF DAAGE AFTER A 7LA i S

3-2i 4

-~~~ ~ ~~~ I-

14 t N

N)

CAA

CONT RO0L t-LTII DRY L AF wl -tiDRY T WG 11EITHANOL EXTRACT 1ITHANOL EXTRACT

- 23 -

Although some degree of efficacy of Agfaia 6p cxtract in

crop prottect on was observed accurate results could not be cheived

dup to the diminishing numbers of larvae both in tie treated and control

experiment

Another uxperiment was then carried out under the Ltter

ccmtr-)Id cond t ions

late 30 March 1988

plac Pon-harvest Centre Chiang Mal University

Sra-l Broccol (25 days after seedling transfer) in clay pot

urrrunded by 1 inch of water in clay tray to prevent the

l rva from escapirng

Nu7yor of I rva 0pot

Pepi arlana -replications of 10 pots

r -r_t II sprayed with control solution 005o Triton in

aqueous solution

2 sprayed with an aqueous emulsion of 2i leaf methanolic

etract and 00O Iriton

T3 prayed with an aqueous emulsion of 2 twig methanolic

extract and 0057 Iriton

Results sumn aried in Ill 19 20 21 22 23

24 -

Ill 19- Compariscn of leaves damage 4 days after

treatment with 27 leaf methanolic extract

iMd Con tro I

Ill 20 Corrp-ison of leaf dami ge 5 days

after treatment with 24 ]eiif

metharnaoIc extract anI conitrol

- 25 shy

1121 - CoPa r I Son of leaf darrage 5 (lays

after trmarment wi th 2 leaf

methanle] ic extrLct (T 2) 2 twig

netha-nclIc extract (T 3 ) and

control (

ATERATM ITH AGLAIA Sp

a a

CONTROL WITh LEAF METHANOL WITH TWIG METHANOL EXTRACT

EXTRACT

i 23 LEA DAA P G- F A - AGLAIA

Uii

-I-I

1 4

ct cl

CONTROL tjITH LEAF METHAINOL

EXTRACTEXTRACT

WITH TI IG M shy THAN O

- 28 shy

RRIEAwaO PLAN

It can be ccn Iuded from Ihe ( iermenLs on Agtaia sp

that thre exI nt a pa qihilIty o usin this plant as an antifeedant

for Srdopten Ulna in agriculture IPlans have been node for the

I 1l1itu i IL

1) Furdther asSess other irrnu latiorn of Agaa 6p in the

field

2) Compare the activity of scopoletin which is the active

antifeedant in thi plant with other analogues

Toward the end of this reporting period we had reassesed

the insecticidol activitv of the plants which had been pieviously

studied and recorded in our earlior report with various forul ations

Pr arable roult r obtained from the prolictinary studies

0 finl period of this project will then be also spent in evaluating

their Tplical ility ira the fields

IlFFpp[ cFE

I Sai Tikwattanit Mc Thesis Kasetsart University(1977)

2Vciha Patuoumotat lSc TIhILis aseLtL rt University

Si172)

5 Rtnolh I a I I h1 Ich~z br t~nt XIAIpp (195f1) 1I209

L an a I 1 I( I) _i 109C

7 thinbaton al LJ Ec-ktt JkrCtem Soc (1912) 94 825d

C i 1 2 a nd Ling JCS

- 29 shy

9 MJBHqluy 2Cruubju 1J lam andc LAWhj t iio JCSChumI

(012 (i12) 1

IU iil 12 ~cit I ~ J03 lnuut Ilivio ( Ijibt) 14 106 3

11 V--1 lni I1~~u Ji)141 L)ad4 -i

1 Al~Kuai NMzkhhi ACuizwk iiI sjuriai Ayr hiil Chem (073) 1y(09

3 K Wda Y Li ato aind FKmnaat Acjr Iiol ChUri 0) (9 34 941

14 EWaija Y Lnuotu and K151Maiio Ag~r ia l Chem (1970) 14 94b

IKatc andu Yjluzaka ta Tet rahedron Le tter (19 74)

17 i v i v a n a d9

(1175) 2 197S

19 KLO2) 1 Miu-a anO C 1iako2j hi 3 CLrCcd(97 6701

20 Iamp Lbo LY-0I KNaLkaishi and ACiiapya JCS

2 1 I ibo SNpjgti Y- Mi ura Nakanislii and AChay

22 Kul~ 1 -4I ( 1 Pet tLe I 1ijwicz and~t~nrih

J C L Cnc1 Coo ) 10 1

23 1 F aboampi F Ha an i sh i T I 1 soh and CillWKa k aW TKuuta

24 7 Iedil Hout- Plan~rt CCC 2 C t-dice to Io it A C S Sympos i Utn

Meriv Uc2 Ameiricajn ChenicoI a00201 gt4 WaiinqLyofl DC (1977)

1 73

PAeIini Plaiin~t JtIC1tacc to l(2tsi CS Stpi r

Series~ U inri can Chemical SOcact washington DC (1977) 374

0

- 30 shy

26 Il Slurkil1 A Dictionary of the Economic Products of the

Hal ay enin-iula 2 VolMs Minitry of Agriculture and Cooprratives

huj ] Lupuira L-urpir (11)(6)

27 H Grair g t i]i I]ant Spc ie Prportedl y Possesing

I(rt-Control Propert ji-An IICUll )atabjaIe Vesource Systems

1nntjtitt irt-~e~ -tCrnter Hn)roluilu (1985)

23 P1 Tuntiwrwh tt ikurl -t al ht chremjftry (1981 )20 1164

29 T Imatayakul -t 11 A t Chrem (1979) 2 71

30 H HitnlI nll j Yrcwoto) Na tura llv Occurring Inecticides ed

7aconon H ann] Croby P( Marcel Dekker New York (1971) 62

31 W Itwers tatural Productf for Innovative Pest Management

ed Whitehead D L and flowers WS Pergqanon Press Oxford

(1903) 52

- 18 shy

11 15 - Third instar larvae from the

attached eggs

II 16 - NUR OF INSECT AFER TREATMENT WITH AGLAIA SP

t

7-iV-

ur j$N

ETH

Z

A

H u4

A

CONTROL

A D S O R B E D O N

12

WITHl LEAF MLIIANOI EXTRACT

ADSORBED ON BENTONITE

N-ET

21 Hour

WITHI FRESHi LEAF

NETiIANO EXTRACT

X

48

WITII DRY LEAF

METHtANOL EXTRACT

- 20 -

Field Test 2

Dte 21 February 1988

P1ace Farrrer plot Leside road leading to Wat Sart Luang Chiang Mal

(rop PrBccor c i oI days aft r seedl ing tran sfer

2w _r ( relicatin - 3 rplications of 1 x 3 m plot

Ir 7et T1 prayed with control solution 005Triton in aqueous

solut Ion

T2sprayed with 101 dry leaf aqueous extract with 0057

Triton

T3 sprayed with 10 dry twig aqueous cxtract with 005

1riton

Results summarized in Ill 17 and Ill 18

Ill 17 - NUMBER OF SECT AFTER TREATIMENT W1H AGLAIA SP

I--

II

DAY

CONTROL WITH DRY LEAF WITH DRY TWIG

METHANOL EXTRACI METHANOL EXTRACT

ISI 8 - -LEAF DAAGE AFTER A 7LA i S

3-2i 4

-~~~ ~ ~~~ I-

14 t N

N)

CAA

CONT RO0L t-LTII DRY L AF wl -tiDRY T WG 11EITHANOL EXTRACT 1ITHANOL EXTRACT

- 23 -

Although some degree of efficacy of Agfaia 6p cxtract in

crop prottect on was observed accurate results could not be cheived

dup to the diminishing numbers of larvae both in tie treated and control

experiment

Another uxperiment was then carried out under the Ltter

ccmtr-)Id cond t ions

late 30 March 1988

plac Pon-harvest Centre Chiang Mal University

Sra-l Broccol (25 days after seedling transfer) in clay pot

urrrunded by 1 inch of water in clay tray to prevent the

l rva from escapirng

Nu7yor of I rva 0pot

Pepi arlana -replications of 10 pots

r -r_t II sprayed with control solution 005o Triton in

aqueous solution

2 sprayed with an aqueous emulsion of 2i leaf methanolic

etract and 00O Iriton

T3 prayed with an aqueous emulsion of 2 twig methanolic

extract and 0057 Iriton

Results sumn aried in Ill 19 20 21 22 23

24 -

Ill 19- Compariscn of leaves damage 4 days after

treatment with 27 leaf methanolic extract

iMd Con tro I

Ill 20 Corrp-ison of leaf dami ge 5 days

after treatment with 24 ]eiif

metharnaoIc extract anI conitrol

- 25 shy

1121 - CoPa r I Son of leaf darrage 5 (lays

after trmarment wi th 2 leaf

methanle] ic extrLct (T 2) 2 twig

netha-nclIc extract (T 3 ) and

control (

ATERATM ITH AGLAIA Sp

a a

CONTROL WITh LEAF METHANOL WITH TWIG METHANOL EXTRACT

EXTRACT

i 23 LEA DAA P G- F A - AGLAIA

Uii

-I-I

1 4

ct cl

CONTROL tjITH LEAF METHAINOL

EXTRACTEXTRACT

WITH TI IG M shy THAN O

- 28 shy

RRIEAwaO PLAN

It can be ccn Iuded from Ihe ( iermenLs on Agtaia sp

that thre exI nt a pa qihilIty o usin this plant as an antifeedant

for Srdopten Ulna in agriculture IPlans have been node for the

I 1l1itu i IL

1) Furdther asSess other irrnu latiorn of Agaa 6p in the

field

2) Compare the activity of scopoletin which is the active

antifeedant in thi plant with other analogues

Toward the end of this reporting period we had reassesed

the insecticidol activitv of the plants which had been pieviously

studied and recorded in our earlior report with various forul ations

Pr arable roult r obtained from the prolictinary studies

0 finl period of this project will then be also spent in evaluating

their Tplical ility ira the fields

IlFFpp[ cFE

I Sai Tikwattanit Mc Thesis Kasetsart University(1977)

2Vciha Patuoumotat lSc TIhILis aseLtL rt University

Si172)

5 Rtnolh I a I I h1 Ich~z br t~nt XIAIpp (195f1) 1I209

L an a I 1 I( I) _i 109C

7 thinbaton al LJ Ec-ktt JkrCtem Soc (1912) 94 825d

C i 1 2 a nd Ling JCS

- 29 shy

9 MJBHqluy 2Cruubju 1J lam andc LAWhj t iio JCSChumI

(012 (i12) 1

IU iil 12 ~cit I ~ J03 lnuut Ilivio ( Ijibt) 14 106 3

11 V--1 lni I1~~u Ji)141 L)ad4 -i

1 Al~Kuai NMzkhhi ACuizwk iiI sjuriai Ayr hiil Chem (073) 1y(09

3 K Wda Y Li ato aind FKmnaat Acjr Iiol ChUri 0) (9 34 941

14 EWaija Y Lnuotu and K151Maiio Ag~r ia l Chem (1970) 14 94b

IKatc andu Yjluzaka ta Tet rahedron Le tter (19 74)

17 i v i v a n a d9

(1175) 2 197S

19 KLO2) 1 Miu-a anO C 1iako2j hi 3 CLrCcd(97 6701

20 Iamp Lbo LY-0I KNaLkaishi and ACiiapya JCS

2 1 I ibo SNpjgti Y- Mi ura Nakanislii and AChay

22 Kul~ 1 -4I ( 1 Pet tLe I 1ijwicz and~t~nrih

J C L Cnc1 Coo ) 10 1

23 1 F aboampi F Ha an i sh i T I 1 soh and CillWKa k aW TKuuta

24 7 Iedil Hout- Plan~rt CCC 2 C t-dice to Io it A C S Sympos i Utn

Meriv Uc2 Ameiricajn ChenicoI a00201 gt4 WaiinqLyofl DC (1977)

1 73

PAeIini Plaiin~t JtIC1tacc to l(2tsi CS Stpi r

Series~ U inri can Chemical SOcact washington DC (1977) 374

0

- 30 shy

26 Il Slurkil1 A Dictionary of the Economic Products of the

Hal ay enin-iula 2 VolMs Minitry of Agriculture and Cooprratives

huj ] Lupuira L-urpir (11)(6)

27 H Grair g t i]i I]ant Spc ie Prportedl y Possesing

I(rt-Control Propert ji-An IICUll )atabjaIe Vesource Systems

1nntjtitt irt-~e~ -tCrnter Hn)roluilu (1985)

23 P1 Tuntiwrwh tt ikurl -t al ht chremjftry (1981 )20 1164

29 T Imatayakul -t 11 A t Chrem (1979) 2 71

30 H HitnlI nll j Yrcwoto) Na tura llv Occurring Inecticides ed

7aconon H ann] Croby P( Marcel Dekker New York (1971) 62

31 W Itwers tatural Productf for Innovative Pest Management

ed Whitehead D L and flowers WS Pergqanon Press Oxford

(1903) 52

II 16 - NUR OF INSECT AFER TREATMENT WITH AGLAIA SP

t

7-iV-

ur j$N

ETH

Z

A

H u4

A

CONTROL

A D S O R B E D O N

12

WITHl LEAF MLIIANOI EXTRACT

ADSORBED ON BENTONITE

N-ET

21 Hour

WITHI FRESHi LEAF

NETiIANO EXTRACT

X

48

WITII DRY LEAF

METHtANOL EXTRACT

- 20 -

Field Test 2

Dte 21 February 1988

P1ace Farrrer plot Leside road leading to Wat Sart Luang Chiang Mal

(rop PrBccor c i oI days aft r seedl ing tran sfer

2w _r ( relicatin - 3 rplications of 1 x 3 m plot

Ir 7et T1 prayed with control solution 005Triton in aqueous

solut Ion

T2sprayed with 101 dry leaf aqueous extract with 0057

Triton

T3 sprayed with 10 dry twig aqueous cxtract with 005

1riton

Results summarized in Ill 17 and Ill 18

Ill 17 - NUMBER OF SECT AFTER TREATIMENT W1H AGLAIA SP

I--

II

DAY

CONTROL WITH DRY LEAF WITH DRY TWIG

METHANOL EXTRACI METHANOL EXTRACT

ISI 8 - -LEAF DAAGE AFTER A 7LA i S

3-2i 4

-~~~ ~ ~~~ I-

14 t N

N)

CAA

CONT RO0L t-LTII DRY L AF wl -tiDRY T WG 11EITHANOL EXTRACT 1ITHANOL EXTRACT

- 23 -

Although some degree of efficacy of Agfaia 6p cxtract in

crop prottect on was observed accurate results could not be cheived

dup to the diminishing numbers of larvae both in tie treated and control

experiment

Another uxperiment was then carried out under the Ltter

ccmtr-)Id cond t ions

late 30 March 1988

plac Pon-harvest Centre Chiang Mal University

Sra-l Broccol (25 days after seedling transfer) in clay pot

urrrunded by 1 inch of water in clay tray to prevent the

l rva from escapirng

Nu7yor of I rva 0pot

Pepi arlana -replications of 10 pots

r -r_t II sprayed with control solution 005o Triton in

aqueous solution

2 sprayed with an aqueous emulsion of 2i leaf methanolic

etract and 00O Iriton

T3 prayed with an aqueous emulsion of 2 twig methanolic

extract and 0057 Iriton

Results sumn aried in Ill 19 20 21 22 23

24 -

Ill 19- Compariscn of leaves damage 4 days after

treatment with 27 leaf methanolic extract

iMd Con tro I

Ill 20 Corrp-ison of leaf dami ge 5 days

after treatment with 24 ]eiif

metharnaoIc extract anI conitrol

- 25 shy

1121 - CoPa r I Son of leaf darrage 5 (lays

after trmarment wi th 2 leaf

methanle] ic extrLct (T 2) 2 twig

netha-nclIc extract (T 3 ) and

control (

ATERATM ITH AGLAIA Sp

a a

CONTROL WITh LEAF METHANOL WITH TWIG METHANOL EXTRACT

EXTRACT

i 23 LEA DAA P G- F A - AGLAIA

Uii

-I-I

1 4

ct cl

CONTROL tjITH LEAF METHAINOL

EXTRACTEXTRACT

WITH TI IG M shy THAN O

- 28 shy

RRIEAwaO PLAN

It can be ccn Iuded from Ihe ( iermenLs on Agtaia sp

that thre exI nt a pa qihilIty o usin this plant as an antifeedant

for Srdopten Ulna in agriculture IPlans have been node for the

I 1l1itu i IL

1) Furdther asSess other irrnu latiorn of Agaa 6p in the

field

2) Compare the activity of scopoletin which is the active

antifeedant in thi plant with other analogues

Toward the end of this reporting period we had reassesed

the insecticidol activitv of the plants which had been pieviously

studied and recorded in our earlior report with various forul ations

Pr arable roult r obtained from the prolictinary studies

0 finl period of this project will then be also spent in evaluating

their Tplical ility ira the fields

IlFFpp[ cFE

I Sai Tikwattanit Mc Thesis Kasetsart University(1977)

2Vciha Patuoumotat lSc TIhILis aseLtL rt University

Si172)

5 Rtnolh I a I I h1 Ich~z br t~nt XIAIpp (195f1) 1I209

L an a I 1 I( I) _i 109C

7 thinbaton al LJ Ec-ktt JkrCtem Soc (1912) 94 825d

C i 1 2 a nd Ling JCS

- 29 shy

9 MJBHqluy 2Cruubju 1J lam andc LAWhj t iio JCSChumI

(012 (i12) 1

IU iil 12 ~cit I ~ J03 lnuut Ilivio ( Ijibt) 14 106 3

11 V--1 lni I1~~u Ji)141 L)ad4 -i

1 Al~Kuai NMzkhhi ACuizwk iiI sjuriai Ayr hiil Chem (073) 1y(09

3 K Wda Y Li ato aind FKmnaat Acjr Iiol ChUri 0) (9 34 941

14 EWaija Y Lnuotu and K151Maiio Ag~r ia l Chem (1970) 14 94b

IKatc andu Yjluzaka ta Tet rahedron Le tter (19 74)

17 i v i v a n a d9

(1175) 2 197S

19 KLO2) 1 Miu-a anO C 1iako2j hi 3 CLrCcd(97 6701

20 Iamp Lbo LY-0I KNaLkaishi and ACiiapya JCS

2 1 I ibo SNpjgti Y- Mi ura Nakanislii and AChay

22 Kul~ 1 -4I ( 1 Pet tLe I 1ijwicz and~t~nrih

J C L Cnc1 Coo ) 10 1

23 1 F aboampi F Ha an i sh i T I 1 soh and CillWKa k aW TKuuta

24 7 Iedil Hout- Plan~rt CCC 2 C t-dice to Io it A C S Sympos i Utn

Meriv Uc2 Ameiricajn ChenicoI a00201 gt4 WaiinqLyofl DC (1977)

1 73

PAeIini Plaiin~t JtIC1tacc to l(2tsi CS Stpi r

Series~ U inri can Chemical SOcact washington DC (1977) 374

0

- 30 shy

26 Il Slurkil1 A Dictionary of the Economic Products of the

Hal ay enin-iula 2 VolMs Minitry of Agriculture and Cooprratives

huj ] Lupuira L-urpir (11)(6)

27 H Grair g t i]i I]ant Spc ie Prportedl y Possesing

I(rt-Control Propert ji-An IICUll )atabjaIe Vesource Systems

1nntjtitt irt-~e~ -tCrnter Hn)roluilu (1985)

23 P1 Tuntiwrwh tt ikurl -t al ht chremjftry (1981 )20 1164

29 T Imatayakul -t 11 A t Chrem (1979) 2 71

30 H HitnlI nll j Yrcwoto) Na tura llv Occurring Inecticides ed

7aconon H ann] Croby P( Marcel Dekker New York (1971) 62

31 W Itwers tatural Productf for Innovative Pest Management

ed Whitehead D L and flowers WS Pergqanon Press Oxford

(1903) 52

- 20 -

Field Test 2

Dte 21 February 1988

P1ace Farrrer plot Leside road leading to Wat Sart Luang Chiang Mal

(rop PrBccor c i oI days aft r seedl ing tran sfer

2w _r ( relicatin - 3 rplications of 1 x 3 m plot

Ir 7et T1 prayed with control solution 005Triton in aqueous

solut Ion

T2sprayed with 101 dry leaf aqueous extract with 0057

Triton

T3 sprayed with 10 dry twig aqueous cxtract with 005

1riton

Results summarized in Ill 17 and Ill 18

Ill 17 - NUMBER OF SECT AFTER TREATIMENT W1H AGLAIA SP

I--

II

DAY

CONTROL WITH DRY LEAF WITH DRY TWIG

METHANOL EXTRACI METHANOL EXTRACT

ISI 8 - -LEAF DAAGE AFTER A 7LA i S

3-2i 4

-~~~ ~ ~~~ I-

14 t N

N)

CAA

CONT RO0L t-LTII DRY L AF wl -tiDRY T WG 11EITHANOL EXTRACT 1ITHANOL EXTRACT

- 23 -

Although some degree of efficacy of Agfaia 6p cxtract in

crop prottect on was observed accurate results could not be cheived

dup to the diminishing numbers of larvae both in tie treated and control

experiment

Another uxperiment was then carried out under the Ltter

ccmtr-)Id cond t ions

late 30 March 1988

plac Pon-harvest Centre Chiang Mal University

Sra-l Broccol (25 days after seedling transfer) in clay pot

urrrunded by 1 inch of water in clay tray to prevent the

l rva from escapirng

Nu7yor of I rva 0pot

Pepi arlana -replications of 10 pots

r -r_t II sprayed with control solution 005o Triton in

aqueous solution

2 sprayed with an aqueous emulsion of 2i leaf methanolic

etract and 00O Iriton

T3 prayed with an aqueous emulsion of 2 twig methanolic

extract and 0057 Iriton

Results sumn aried in Ill 19 20 21 22 23

24 -

Ill 19- Compariscn of leaves damage 4 days after

treatment with 27 leaf methanolic extract

iMd Con tro I

Ill 20 Corrp-ison of leaf dami ge 5 days

after treatment with 24 ]eiif

metharnaoIc extract anI conitrol

- 25 shy

1121 - CoPa r I Son of leaf darrage 5 (lays

after trmarment wi th 2 leaf

methanle] ic extrLct (T 2) 2 twig

netha-nclIc extract (T 3 ) and

control (

ATERATM ITH AGLAIA Sp

a a

CONTROL WITh LEAF METHANOL WITH TWIG METHANOL EXTRACT

EXTRACT

i 23 LEA DAA P G- F A - AGLAIA

Uii

-I-I

1 4

ct cl

CONTROL tjITH LEAF METHAINOL

EXTRACTEXTRACT

WITH TI IG M shy THAN O

- 28 shy

RRIEAwaO PLAN

It can be ccn Iuded from Ihe ( iermenLs on Agtaia sp

that thre exI nt a pa qihilIty o usin this plant as an antifeedant

for Srdopten Ulna in agriculture IPlans have been node for the

I 1l1itu i IL

1) Furdther asSess other irrnu latiorn of Agaa 6p in the

field

2) Compare the activity of scopoletin which is the active

antifeedant in thi plant with other analogues

Toward the end of this reporting period we had reassesed

the insecticidol activitv of the plants which had been pieviously

studied and recorded in our earlior report with various forul ations

Pr arable roult r obtained from the prolictinary studies

0 finl period of this project will then be also spent in evaluating

their Tplical ility ira the fields

IlFFpp[ cFE

I Sai Tikwattanit Mc Thesis Kasetsart University(1977)

2Vciha Patuoumotat lSc TIhILis aseLtL rt University

Si172)

5 Rtnolh I a I I h1 Ich~z br t~nt XIAIpp (195f1) 1I209

L an a I 1 I( I) _i 109C

7 thinbaton al LJ Ec-ktt JkrCtem Soc (1912) 94 825d

C i 1 2 a nd Ling JCS

- 29 shy

9 MJBHqluy 2Cruubju 1J lam andc LAWhj t iio JCSChumI

(012 (i12) 1

IU iil 12 ~cit I ~ J03 lnuut Ilivio ( Ijibt) 14 106 3

11 V--1 lni I1~~u Ji)141 L)ad4 -i

1 Al~Kuai NMzkhhi ACuizwk iiI sjuriai Ayr hiil Chem (073) 1y(09

3 K Wda Y Li ato aind FKmnaat Acjr Iiol ChUri 0) (9 34 941

14 EWaija Y Lnuotu and K151Maiio Ag~r ia l Chem (1970) 14 94b

IKatc andu Yjluzaka ta Tet rahedron Le tter (19 74)

17 i v i v a n a d9

(1175) 2 197S

19 KLO2) 1 Miu-a anO C 1iako2j hi 3 CLrCcd(97 6701

20 Iamp Lbo LY-0I KNaLkaishi and ACiiapya JCS

2 1 I ibo SNpjgti Y- Mi ura Nakanislii and AChay

22 Kul~ 1 -4I ( 1 Pet tLe I 1ijwicz and~t~nrih

J C L Cnc1 Coo ) 10 1

23 1 F aboampi F Ha an i sh i T I 1 soh and CillWKa k aW TKuuta

24 7 Iedil Hout- Plan~rt CCC 2 C t-dice to Io it A C S Sympos i Utn

Meriv Uc2 Ameiricajn ChenicoI a00201 gt4 WaiinqLyofl DC (1977)

1 73

PAeIini Plaiin~t JtIC1tacc to l(2tsi CS Stpi r

Series~ U inri can Chemical SOcact washington DC (1977) 374

0

- 30 shy

26 Il Slurkil1 A Dictionary of the Economic Products of the

Hal ay enin-iula 2 VolMs Minitry of Agriculture and Cooprratives

huj ] Lupuira L-urpir (11)(6)

27 H Grair g t i]i I]ant Spc ie Prportedl y Possesing

I(rt-Control Propert ji-An IICUll )atabjaIe Vesource Systems

1nntjtitt irt-~e~ -tCrnter Hn)roluilu (1985)

23 P1 Tuntiwrwh tt ikurl -t al ht chremjftry (1981 )20 1164

29 T Imatayakul -t 11 A t Chrem (1979) 2 71

30 H HitnlI nll j Yrcwoto) Na tura llv Occurring Inecticides ed

7aconon H ann] Croby P( Marcel Dekker New York (1971) 62

31 W Itwers tatural Productf for Innovative Pest Management

ed Whitehead D L and flowers WS Pergqanon Press Oxford

(1903) 52

Ill 17 - NUMBER OF SECT AFTER TREATIMENT W1H AGLAIA SP

I--

II

DAY

CONTROL WITH DRY LEAF WITH DRY TWIG

METHANOL EXTRACI METHANOL EXTRACT

ISI 8 - -LEAF DAAGE AFTER A 7LA i S

3-2i 4

-~~~ ~ ~~~ I-

14 t N

N)

CAA

CONT RO0L t-LTII DRY L AF wl -tiDRY T WG 11EITHANOL EXTRACT 1ITHANOL EXTRACT

- 23 -

Although some degree of efficacy of Agfaia 6p cxtract in

crop prottect on was observed accurate results could not be cheived

dup to the diminishing numbers of larvae both in tie treated and control

experiment

Another uxperiment was then carried out under the Ltter

ccmtr-)Id cond t ions

late 30 March 1988

plac Pon-harvest Centre Chiang Mal University

Sra-l Broccol (25 days after seedling transfer) in clay pot

urrrunded by 1 inch of water in clay tray to prevent the

l rva from escapirng

Nu7yor of I rva 0pot

Pepi arlana -replications of 10 pots

r -r_t II sprayed with control solution 005o Triton in

aqueous solution

2 sprayed with an aqueous emulsion of 2i leaf methanolic

etract and 00O Iriton

T3 prayed with an aqueous emulsion of 2 twig methanolic

extract and 0057 Iriton

Results sumn aried in Ill 19 20 21 22 23

24 -

Ill 19- Compariscn of leaves damage 4 days after

treatment with 27 leaf methanolic extract

iMd Con tro I

Ill 20 Corrp-ison of leaf dami ge 5 days

after treatment with 24 ]eiif

metharnaoIc extract anI conitrol

- 25 shy

1121 - CoPa r I Son of leaf darrage 5 (lays

after trmarment wi th 2 leaf

methanle] ic extrLct (T 2) 2 twig

netha-nclIc extract (T 3 ) and

control (

ATERATM ITH AGLAIA Sp

a a

CONTROL WITh LEAF METHANOL WITH TWIG METHANOL EXTRACT

EXTRACT

i 23 LEA DAA P G- F A - AGLAIA

Uii

-I-I

1 4

ct cl

CONTROL tjITH LEAF METHAINOL

EXTRACTEXTRACT

WITH TI IG M shy THAN O

- 28 shy

RRIEAwaO PLAN

It can be ccn Iuded from Ihe ( iermenLs on Agtaia sp

that thre exI nt a pa qihilIty o usin this plant as an antifeedant

for Srdopten Ulna in agriculture IPlans have been node for the

I 1l1itu i IL

1) Furdther asSess other irrnu latiorn of Agaa 6p in the

field

2) Compare the activity of scopoletin which is the active

antifeedant in thi plant with other analogues

Toward the end of this reporting period we had reassesed

the insecticidol activitv of the plants which had been pieviously

studied and recorded in our earlior report with various forul ations

Pr arable roult r obtained from the prolictinary studies

0 finl period of this project will then be also spent in evaluating

their Tplical ility ira the fields

IlFFpp[ cFE

I Sai Tikwattanit Mc Thesis Kasetsart University(1977)

2Vciha Patuoumotat lSc TIhILis aseLtL rt University

Si172)

5 Rtnolh I a I I h1 Ich~z br t~nt XIAIpp (195f1) 1I209

L an a I 1 I( I) _i 109C

7 thinbaton al LJ Ec-ktt JkrCtem Soc (1912) 94 825d

C i 1 2 a nd Ling JCS

- 29 shy

9 MJBHqluy 2Cruubju 1J lam andc LAWhj t iio JCSChumI

(012 (i12) 1

IU iil 12 ~cit I ~ J03 lnuut Ilivio ( Ijibt) 14 106 3

11 V--1 lni I1~~u Ji)141 L)ad4 -i

1 Al~Kuai NMzkhhi ACuizwk iiI sjuriai Ayr hiil Chem (073) 1y(09

3 K Wda Y Li ato aind FKmnaat Acjr Iiol ChUri 0) (9 34 941

14 EWaija Y Lnuotu and K151Maiio Ag~r ia l Chem (1970) 14 94b

IKatc andu Yjluzaka ta Tet rahedron Le tter (19 74)

17 i v i v a n a d9

(1175) 2 197S

19 KLO2) 1 Miu-a anO C 1iako2j hi 3 CLrCcd(97 6701

20 Iamp Lbo LY-0I KNaLkaishi and ACiiapya JCS

2 1 I ibo SNpjgti Y- Mi ura Nakanislii and AChay

22 Kul~ 1 -4I ( 1 Pet tLe I 1ijwicz and~t~nrih

J C L Cnc1 Coo ) 10 1

23 1 F aboampi F Ha an i sh i T I 1 soh and CillWKa k aW TKuuta

24 7 Iedil Hout- Plan~rt CCC 2 C t-dice to Io it A C S Sympos i Utn

Meriv Uc2 Ameiricajn ChenicoI a00201 gt4 WaiinqLyofl DC (1977)

1 73

PAeIini Plaiin~t JtIC1tacc to l(2tsi CS Stpi r

Series~ U inri can Chemical SOcact washington DC (1977) 374

0

- 30 shy

26 Il Slurkil1 A Dictionary of the Economic Products of the

Hal ay enin-iula 2 VolMs Minitry of Agriculture and Cooprratives

huj ] Lupuira L-urpir (11)(6)

27 H Grair g t i]i I]ant Spc ie Prportedl y Possesing

I(rt-Control Propert ji-An IICUll )atabjaIe Vesource Systems

1nntjtitt irt-~e~ -tCrnter Hn)roluilu (1985)

23 P1 Tuntiwrwh tt ikurl -t al ht chremjftry (1981 )20 1164

29 T Imatayakul -t 11 A t Chrem (1979) 2 71

30 H HitnlI nll j Yrcwoto) Na tura llv Occurring Inecticides ed

7aconon H ann] Croby P( Marcel Dekker New York (1971) 62

31 W Itwers tatural Productf for Innovative Pest Management

ed Whitehead D L and flowers WS Pergqanon Press Oxford

(1903) 52

ISI 8 - -LEAF DAAGE AFTER A 7LA i S

3-2i 4

-~~~ ~ ~~~ I-

14 t N

N)

CAA

CONT RO0L t-LTII DRY L AF wl -tiDRY T WG 11EITHANOL EXTRACT 1ITHANOL EXTRACT

- 23 -

Although some degree of efficacy of Agfaia 6p cxtract in

crop prottect on was observed accurate results could not be cheived

dup to the diminishing numbers of larvae both in tie treated and control

experiment

Another uxperiment was then carried out under the Ltter

ccmtr-)Id cond t ions

late 30 March 1988

plac Pon-harvest Centre Chiang Mal University

Sra-l Broccol (25 days after seedling transfer) in clay pot

urrrunded by 1 inch of water in clay tray to prevent the

l rva from escapirng

Nu7yor of I rva 0pot

Pepi arlana -replications of 10 pots

r -r_t II sprayed with control solution 005o Triton in

aqueous solution

2 sprayed with an aqueous emulsion of 2i leaf methanolic

etract and 00O Iriton

T3 prayed with an aqueous emulsion of 2 twig methanolic

extract and 0057 Iriton

Results sumn aried in Ill 19 20 21 22 23

24 -

Ill 19- Compariscn of leaves damage 4 days after

treatment with 27 leaf methanolic extract

iMd Con tro I

Ill 20 Corrp-ison of leaf dami ge 5 days

after treatment with 24 ]eiif

metharnaoIc extract anI conitrol

- 25 shy

1121 - CoPa r I Son of leaf darrage 5 (lays

after trmarment wi th 2 leaf

methanle] ic extrLct (T 2) 2 twig

netha-nclIc extract (T 3 ) and

control (

ATERATM ITH AGLAIA Sp

a a

CONTROL WITh LEAF METHANOL WITH TWIG METHANOL EXTRACT

EXTRACT

i 23 LEA DAA P G- F A - AGLAIA

Uii

-I-I

1 4

ct cl

CONTROL tjITH LEAF METHAINOL

EXTRACTEXTRACT

WITH TI IG M shy THAN O

- 28 shy

RRIEAwaO PLAN

It can be ccn Iuded from Ihe ( iermenLs on Agtaia sp

that thre exI nt a pa qihilIty o usin this plant as an antifeedant

for Srdopten Ulna in agriculture IPlans have been node for the

I 1l1itu i IL

1) Furdther asSess other irrnu latiorn of Agaa 6p in the

field

2) Compare the activity of scopoletin which is the active

antifeedant in thi plant with other analogues

Toward the end of this reporting period we had reassesed

the insecticidol activitv of the plants which had been pieviously

studied and recorded in our earlior report with various forul ations

Pr arable roult r obtained from the prolictinary studies

0 finl period of this project will then be also spent in evaluating

their Tplical ility ira the fields

IlFFpp[ cFE

I Sai Tikwattanit Mc Thesis Kasetsart University(1977)

2Vciha Patuoumotat lSc TIhILis aseLtL rt University

Si172)

5 Rtnolh I a I I h1 Ich~z br t~nt XIAIpp (195f1) 1I209

L an a I 1 I( I) _i 109C

7 thinbaton al LJ Ec-ktt JkrCtem Soc (1912) 94 825d

C i 1 2 a nd Ling JCS

- 29 shy

9 MJBHqluy 2Cruubju 1J lam andc LAWhj t iio JCSChumI

(012 (i12) 1

IU iil 12 ~cit I ~ J03 lnuut Ilivio ( Ijibt) 14 106 3

11 V--1 lni I1~~u Ji)141 L)ad4 -i

1 Al~Kuai NMzkhhi ACuizwk iiI sjuriai Ayr hiil Chem (073) 1y(09

3 K Wda Y Li ato aind FKmnaat Acjr Iiol ChUri 0) (9 34 941

14 EWaija Y Lnuotu and K151Maiio Ag~r ia l Chem (1970) 14 94b

IKatc andu Yjluzaka ta Tet rahedron Le tter (19 74)

17 i v i v a n a d9

(1175) 2 197S

19 KLO2) 1 Miu-a anO C 1iako2j hi 3 CLrCcd(97 6701

20 Iamp Lbo LY-0I KNaLkaishi and ACiiapya JCS

2 1 I ibo SNpjgti Y- Mi ura Nakanislii and AChay

22 Kul~ 1 -4I ( 1 Pet tLe I 1ijwicz and~t~nrih

J C L Cnc1 Coo ) 10 1

23 1 F aboampi F Ha an i sh i T I 1 soh and CillWKa k aW TKuuta

24 7 Iedil Hout- Plan~rt CCC 2 C t-dice to Io it A C S Sympos i Utn

Meriv Uc2 Ameiricajn ChenicoI a00201 gt4 WaiinqLyofl DC (1977)

1 73

PAeIini Plaiin~t JtIC1tacc to l(2tsi CS Stpi r

Series~ U inri can Chemical SOcact washington DC (1977) 374

0

- 30 shy

26 Il Slurkil1 A Dictionary of the Economic Products of the

Hal ay enin-iula 2 VolMs Minitry of Agriculture and Cooprratives

huj ] Lupuira L-urpir (11)(6)

27 H Grair g t i]i I]ant Spc ie Prportedl y Possesing

I(rt-Control Propert ji-An IICUll )atabjaIe Vesource Systems

1nntjtitt irt-~e~ -tCrnter Hn)roluilu (1985)

23 P1 Tuntiwrwh tt ikurl -t al ht chremjftry (1981 )20 1164

29 T Imatayakul -t 11 A t Chrem (1979) 2 71

30 H HitnlI nll j Yrcwoto) Na tura llv Occurring Inecticides ed

7aconon H ann] Croby P( Marcel Dekker New York (1971) 62

31 W Itwers tatural Productf for Innovative Pest Management

ed Whitehead D L and flowers WS Pergqanon Press Oxford

(1903) 52

- 23 -

Although some degree of efficacy of Agfaia 6p cxtract in

crop prottect on was observed accurate results could not be cheived

dup to the diminishing numbers of larvae both in tie treated and control

experiment

Another uxperiment was then carried out under the Ltter

ccmtr-)Id cond t ions

late 30 March 1988

plac Pon-harvest Centre Chiang Mal University

Sra-l Broccol (25 days after seedling transfer) in clay pot

urrrunded by 1 inch of water in clay tray to prevent the

l rva from escapirng

Nu7yor of I rva 0pot

Pepi arlana -replications of 10 pots

r -r_t II sprayed with control solution 005o Triton in

aqueous solution

2 sprayed with an aqueous emulsion of 2i leaf methanolic

etract and 00O Iriton

T3 prayed with an aqueous emulsion of 2 twig methanolic

extract and 0057 Iriton

Results sumn aried in Ill 19 20 21 22 23

24 -

Ill 19- Compariscn of leaves damage 4 days after

treatment with 27 leaf methanolic extract

iMd Con tro I

Ill 20 Corrp-ison of leaf dami ge 5 days

after treatment with 24 ]eiif

metharnaoIc extract anI conitrol

- 25 shy

1121 - CoPa r I Son of leaf darrage 5 (lays

after trmarment wi th 2 leaf

methanle] ic extrLct (T 2) 2 twig

netha-nclIc extract (T 3 ) and

control (

ATERATM ITH AGLAIA Sp

a a

CONTROL WITh LEAF METHANOL WITH TWIG METHANOL EXTRACT

EXTRACT

i 23 LEA DAA P G- F A - AGLAIA

Uii

-I-I

1 4

ct cl

CONTROL tjITH LEAF METHAINOL

EXTRACTEXTRACT

WITH TI IG M shy THAN O

- 28 shy

RRIEAwaO PLAN

It can be ccn Iuded from Ihe ( iermenLs on Agtaia sp

that thre exI nt a pa qihilIty o usin this plant as an antifeedant

for Srdopten Ulna in agriculture IPlans have been node for the

I 1l1itu i IL

1) Furdther asSess other irrnu latiorn of Agaa 6p in the

field

2) Compare the activity of scopoletin which is the active

antifeedant in thi plant with other analogues

Toward the end of this reporting period we had reassesed

the insecticidol activitv of the plants which had been pieviously

studied and recorded in our earlior report with various forul ations

Pr arable roult r obtained from the prolictinary studies

0 finl period of this project will then be also spent in evaluating

their Tplical ility ira the fields

IlFFpp[ cFE

I Sai Tikwattanit Mc Thesis Kasetsart University(1977)

2Vciha Patuoumotat lSc TIhILis aseLtL rt University

Si172)

5 Rtnolh I a I I h1 Ich~z br t~nt XIAIpp (195f1) 1I209

L an a I 1 I( I) _i 109C

7 thinbaton al LJ Ec-ktt JkrCtem Soc (1912) 94 825d

C i 1 2 a nd Ling JCS

- 29 shy

9 MJBHqluy 2Cruubju 1J lam andc LAWhj t iio JCSChumI

(012 (i12) 1

IU iil 12 ~cit I ~ J03 lnuut Ilivio ( Ijibt) 14 106 3

11 V--1 lni I1~~u Ji)141 L)ad4 -i

1 Al~Kuai NMzkhhi ACuizwk iiI sjuriai Ayr hiil Chem (073) 1y(09

3 K Wda Y Li ato aind FKmnaat Acjr Iiol ChUri 0) (9 34 941

14 EWaija Y Lnuotu and K151Maiio Ag~r ia l Chem (1970) 14 94b

IKatc andu Yjluzaka ta Tet rahedron Le tter (19 74)

17 i v i v a n a d9

(1175) 2 197S

19 KLO2) 1 Miu-a anO C 1iako2j hi 3 CLrCcd(97 6701

20 Iamp Lbo LY-0I KNaLkaishi and ACiiapya JCS

2 1 I ibo SNpjgti Y- Mi ura Nakanislii and AChay

22 Kul~ 1 -4I ( 1 Pet tLe I 1ijwicz and~t~nrih

J C L Cnc1 Coo ) 10 1

23 1 F aboampi F Ha an i sh i T I 1 soh and CillWKa k aW TKuuta

24 7 Iedil Hout- Plan~rt CCC 2 C t-dice to Io it A C S Sympos i Utn

Meriv Uc2 Ameiricajn ChenicoI a00201 gt4 WaiinqLyofl DC (1977)

1 73

PAeIini Plaiin~t JtIC1tacc to l(2tsi CS Stpi r

Series~ U inri can Chemical SOcact washington DC (1977) 374

0

- 30 shy

26 Il Slurkil1 A Dictionary of the Economic Products of the

Hal ay enin-iula 2 VolMs Minitry of Agriculture and Cooprratives

huj ] Lupuira L-urpir (11)(6)

27 H Grair g t i]i I]ant Spc ie Prportedl y Possesing

I(rt-Control Propert ji-An IICUll )atabjaIe Vesource Systems

1nntjtitt irt-~e~ -tCrnter Hn)roluilu (1985)

23 P1 Tuntiwrwh tt ikurl -t al ht chremjftry (1981 )20 1164

29 T Imatayakul -t 11 A t Chrem (1979) 2 71

30 H HitnlI nll j Yrcwoto) Na tura llv Occurring Inecticides ed

7aconon H ann] Croby P( Marcel Dekker New York (1971) 62

31 W Itwers tatural Productf for Innovative Pest Management

ed Whitehead D L and flowers WS Pergqanon Press Oxford

(1903) 52

24 -

Ill 19- Compariscn of leaves damage 4 days after

treatment with 27 leaf methanolic extract

iMd Con tro I

Ill 20 Corrp-ison of leaf dami ge 5 days

after treatment with 24 ]eiif

metharnaoIc extract anI conitrol

- 25 shy

1121 - CoPa r I Son of leaf darrage 5 (lays

after trmarment wi th 2 leaf

methanle] ic extrLct (T 2) 2 twig

netha-nclIc extract (T 3 ) and

control (

ATERATM ITH AGLAIA Sp

a a

CONTROL WITh LEAF METHANOL WITH TWIG METHANOL EXTRACT

EXTRACT

i 23 LEA DAA P G- F A - AGLAIA

Uii

-I-I

1 4

ct cl

CONTROL tjITH LEAF METHAINOL

EXTRACTEXTRACT

WITH TI IG M shy THAN O

- 28 shy

RRIEAwaO PLAN

It can be ccn Iuded from Ihe ( iermenLs on Agtaia sp

that thre exI nt a pa qihilIty o usin this plant as an antifeedant

for Srdopten Ulna in agriculture IPlans have been node for the

I 1l1itu i IL

1) Furdther asSess other irrnu latiorn of Agaa 6p in the

field

2) Compare the activity of scopoletin which is the active

antifeedant in thi plant with other analogues

Toward the end of this reporting period we had reassesed

the insecticidol activitv of the plants which had been pieviously

studied and recorded in our earlior report with various forul ations

Pr arable roult r obtained from the prolictinary studies

0 finl period of this project will then be also spent in evaluating

their Tplical ility ira the fields

IlFFpp[ cFE

I Sai Tikwattanit Mc Thesis Kasetsart University(1977)

2Vciha Patuoumotat lSc TIhILis aseLtL rt University

Si172)

5 Rtnolh I a I I h1 Ich~z br t~nt XIAIpp (195f1) 1I209

L an a I 1 I( I) _i 109C

7 thinbaton al LJ Ec-ktt JkrCtem Soc (1912) 94 825d

C i 1 2 a nd Ling JCS

- 29 shy

9 MJBHqluy 2Cruubju 1J lam andc LAWhj t iio JCSChumI

(012 (i12) 1

IU iil 12 ~cit I ~ J03 lnuut Ilivio ( Ijibt) 14 106 3

11 V--1 lni I1~~u Ji)141 L)ad4 -i

1 Al~Kuai NMzkhhi ACuizwk iiI sjuriai Ayr hiil Chem (073) 1y(09

3 K Wda Y Li ato aind FKmnaat Acjr Iiol ChUri 0) (9 34 941

14 EWaija Y Lnuotu and K151Maiio Ag~r ia l Chem (1970) 14 94b

IKatc andu Yjluzaka ta Tet rahedron Le tter (19 74)

17 i v i v a n a d9

(1175) 2 197S

19 KLO2) 1 Miu-a anO C 1iako2j hi 3 CLrCcd(97 6701

20 Iamp Lbo LY-0I KNaLkaishi and ACiiapya JCS

2 1 I ibo SNpjgti Y- Mi ura Nakanislii and AChay

22 Kul~ 1 -4I ( 1 Pet tLe I 1ijwicz and~t~nrih

J C L Cnc1 Coo ) 10 1

23 1 F aboampi F Ha an i sh i T I 1 soh and CillWKa k aW TKuuta

24 7 Iedil Hout- Plan~rt CCC 2 C t-dice to Io it A C S Sympos i Utn

Meriv Uc2 Ameiricajn ChenicoI a00201 gt4 WaiinqLyofl DC (1977)

1 73

PAeIini Plaiin~t JtIC1tacc to l(2tsi CS Stpi r

Series~ U inri can Chemical SOcact washington DC (1977) 374

0

- 30 shy

26 Il Slurkil1 A Dictionary of the Economic Products of the

Hal ay enin-iula 2 VolMs Minitry of Agriculture and Cooprratives

huj ] Lupuira L-urpir (11)(6)

27 H Grair g t i]i I]ant Spc ie Prportedl y Possesing

I(rt-Control Propert ji-An IICUll )atabjaIe Vesource Systems

1nntjtitt irt-~e~ -tCrnter Hn)roluilu (1985)

23 P1 Tuntiwrwh tt ikurl -t al ht chremjftry (1981 )20 1164

29 T Imatayakul -t 11 A t Chrem (1979) 2 71

30 H HitnlI nll j Yrcwoto) Na tura llv Occurring Inecticides ed

7aconon H ann] Croby P( Marcel Dekker New York (1971) 62

31 W Itwers tatural Productf for Innovative Pest Management

ed Whitehead D L and flowers WS Pergqanon Press Oxford

(1903) 52

- 25 shy

1121 - CoPa r I Son of leaf darrage 5 (lays

after trmarment wi th 2 leaf

methanle] ic extrLct (T 2) 2 twig

netha-nclIc extract (T 3 ) and

control (

ATERATM ITH AGLAIA Sp

a a

CONTROL WITh LEAF METHANOL WITH TWIG METHANOL EXTRACT

EXTRACT

i 23 LEA DAA P G- F A - AGLAIA

Uii

-I-I

1 4

ct cl

CONTROL tjITH LEAF METHAINOL

EXTRACTEXTRACT

WITH TI IG M shy THAN O

- 28 shy

RRIEAwaO PLAN

It can be ccn Iuded from Ihe ( iermenLs on Agtaia sp

that thre exI nt a pa qihilIty o usin this plant as an antifeedant

for Srdopten Ulna in agriculture IPlans have been node for the

I 1l1itu i IL

1) Furdther asSess other irrnu latiorn of Agaa 6p in the

field

2) Compare the activity of scopoletin which is the active

antifeedant in thi plant with other analogues

Toward the end of this reporting period we had reassesed

the insecticidol activitv of the plants which had been pieviously

studied and recorded in our earlior report with various forul ations

Pr arable roult r obtained from the prolictinary studies

0 finl period of this project will then be also spent in evaluating

their Tplical ility ira the fields

IlFFpp[ cFE

I Sai Tikwattanit Mc Thesis Kasetsart University(1977)

2Vciha Patuoumotat lSc TIhILis aseLtL rt University

Si172)

5 Rtnolh I a I I h1 Ich~z br t~nt XIAIpp (195f1) 1I209

L an a I 1 I( I) _i 109C

7 thinbaton al LJ Ec-ktt JkrCtem Soc (1912) 94 825d

C i 1 2 a nd Ling JCS

- 29 shy

9 MJBHqluy 2Cruubju 1J lam andc LAWhj t iio JCSChumI

(012 (i12) 1

IU iil 12 ~cit I ~ J03 lnuut Ilivio ( Ijibt) 14 106 3

11 V--1 lni I1~~u Ji)141 L)ad4 -i

1 Al~Kuai NMzkhhi ACuizwk iiI sjuriai Ayr hiil Chem (073) 1y(09

3 K Wda Y Li ato aind FKmnaat Acjr Iiol ChUri 0) (9 34 941

14 EWaija Y Lnuotu and K151Maiio Ag~r ia l Chem (1970) 14 94b

IKatc andu Yjluzaka ta Tet rahedron Le tter (19 74)

17 i v i v a n a d9

(1175) 2 197S

19 KLO2) 1 Miu-a anO C 1iako2j hi 3 CLrCcd(97 6701

20 Iamp Lbo LY-0I KNaLkaishi and ACiiapya JCS

2 1 I ibo SNpjgti Y- Mi ura Nakanislii and AChay

22 Kul~ 1 -4I ( 1 Pet tLe I 1ijwicz and~t~nrih

J C L Cnc1 Coo ) 10 1

23 1 F aboampi F Ha an i sh i T I 1 soh and CillWKa k aW TKuuta

24 7 Iedil Hout- Plan~rt CCC 2 C t-dice to Io it A C S Sympos i Utn

Meriv Uc2 Ameiricajn ChenicoI a00201 gt4 WaiinqLyofl DC (1977)

1 73

PAeIini Plaiin~t JtIC1tacc to l(2tsi CS Stpi r

Series~ U inri can Chemical SOcact washington DC (1977) 374

0

- 30 shy

26 Il Slurkil1 A Dictionary of the Economic Products of the

Hal ay enin-iula 2 VolMs Minitry of Agriculture and Cooprratives

huj ] Lupuira L-urpir (11)(6)

27 H Grair g t i]i I]ant Spc ie Prportedl y Possesing

I(rt-Control Propert ji-An IICUll )atabjaIe Vesource Systems

1nntjtitt irt-~e~ -tCrnter Hn)roluilu (1985)

23 P1 Tuntiwrwh tt ikurl -t al ht chremjftry (1981 )20 1164

29 T Imatayakul -t 11 A t Chrem (1979) 2 71

30 H HitnlI nll j Yrcwoto) Na tura llv Occurring Inecticides ed

7aconon H ann] Croby P( Marcel Dekker New York (1971) 62

31 W Itwers tatural Productf for Innovative Pest Management

ed Whitehead D L and flowers WS Pergqanon Press Oxford

(1903) 52

ATERATM ITH AGLAIA Sp

a a

CONTROL WITh LEAF METHANOL WITH TWIG METHANOL EXTRACT

EXTRACT

i 23 LEA DAA P G- F A - AGLAIA

Uii

-I-I

1 4

ct cl

CONTROL tjITH LEAF METHAINOL

EXTRACTEXTRACT

WITH TI IG M shy THAN O

- 28 shy

RRIEAwaO PLAN

It can be ccn Iuded from Ihe ( iermenLs on Agtaia sp

that thre exI nt a pa qihilIty o usin this plant as an antifeedant

for Srdopten Ulna in agriculture IPlans have been node for the

I 1l1itu i IL

1) Furdther asSess other irrnu latiorn of Agaa 6p in the

field

2) Compare the activity of scopoletin which is the active

antifeedant in thi plant with other analogues

Toward the end of this reporting period we had reassesed

the insecticidol activitv of the plants which had been pieviously

studied and recorded in our earlior report with various forul ations

Pr arable roult r obtained from the prolictinary studies

0 finl period of this project will then be also spent in evaluating

their Tplical ility ira the fields

IlFFpp[ cFE

I Sai Tikwattanit Mc Thesis Kasetsart University(1977)

2Vciha Patuoumotat lSc TIhILis aseLtL rt University

Si172)

5 Rtnolh I a I I h1 Ich~z br t~nt XIAIpp (195f1) 1I209

L an a I 1 I( I) _i 109C

7 thinbaton al LJ Ec-ktt JkrCtem Soc (1912) 94 825d

C i 1 2 a nd Ling JCS

- 29 shy

9 MJBHqluy 2Cruubju 1J lam andc LAWhj t iio JCSChumI

(012 (i12) 1

IU iil 12 ~cit I ~ J03 lnuut Ilivio ( Ijibt) 14 106 3

11 V--1 lni I1~~u Ji)141 L)ad4 -i

1 Al~Kuai NMzkhhi ACuizwk iiI sjuriai Ayr hiil Chem (073) 1y(09

3 K Wda Y Li ato aind FKmnaat Acjr Iiol ChUri 0) (9 34 941

14 EWaija Y Lnuotu and K151Maiio Ag~r ia l Chem (1970) 14 94b

IKatc andu Yjluzaka ta Tet rahedron Le tter (19 74)

17 i v i v a n a d9

(1175) 2 197S

19 KLO2) 1 Miu-a anO C 1iako2j hi 3 CLrCcd(97 6701

20 Iamp Lbo LY-0I KNaLkaishi and ACiiapya JCS

2 1 I ibo SNpjgti Y- Mi ura Nakanislii and AChay

22 Kul~ 1 -4I ( 1 Pet tLe I 1ijwicz and~t~nrih

J C L Cnc1 Coo ) 10 1

23 1 F aboampi F Ha an i sh i T I 1 soh and CillWKa k aW TKuuta

24 7 Iedil Hout- Plan~rt CCC 2 C t-dice to Io it A C S Sympos i Utn

Meriv Uc2 Ameiricajn ChenicoI a00201 gt4 WaiinqLyofl DC (1977)

1 73

PAeIini Plaiin~t JtIC1tacc to l(2tsi CS Stpi r

Series~ U inri can Chemical SOcact washington DC (1977) 374

0

- 30 shy

26 Il Slurkil1 A Dictionary of the Economic Products of the

Hal ay enin-iula 2 VolMs Minitry of Agriculture and Cooprratives

huj ] Lupuira L-urpir (11)(6)

27 H Grair g t i]i I]ant Spc ie Prportedl y Possesing

I(rt-Control Propert ji-An IICUll )atabjaIe Vesource Systems

1nntjtitt irt-~e~ -tCrnter Hn)roluilu (1985)

23 P1 Tuntiwrwh tt ikurl -t al ht chremjftry (1981 )20 1164

29 T Imatayakul -t 11 A t Chrem (1979) 2 71

30 H HitnlI nll j Yrcwoto) Na tura llv Occurring Inecticides ed

7aconon H ann] Croby P( Marcel Dekker New York (1971) 62

31 W Itwers tatural Productf for Innovative Pest Management

ed Whitehead D L and flowers WS Pergqanon Press Oxford

(1903) 52

i 23 LEA DAA P G- F A - AGLAIA

Uii

-I-I

1 4

ct cl

CONTROL tjITH LEAF METHAINOL

EXTRACTEXTRACT

WITH TI IG M shy THAN O

- 28 shy

RRIEAwaO PLAN

It can be ccn Iuded from Ihe ( iermenLs on Agtaia sp

that thre exI nt a pa qihilIty o usin this plant as an antifeedant

for Srdopten Ulna in agriculture IPlans have been node for the

I 1l1itu i IL

1) Furdther asSess other irrnu latiorn of Agaa 6p in the

field

2) Compare the activity of scopoletin which is the active

antifeedant in thi plant with other analogues

Toward the end of this reporting period we had reassesed

the insecticidol activitv of the plants which had been pieviously

studied and recorded in our earlior report with various forul ations

Pr arable roult r obtained from the prolictinary studies

0 finl period of this project will then be also spent in evaluating

their Tplical ility ira the fields

IlFFpp[ cFE

I Sai Tikwattanit Mc Thesis Kasetsart University(1977)

2Vciha Patuoumotat lSc TIhILis aseLtL rt University

Si172)

5 Rtnolh I a I I h1 Ich~z br t~nt XIAIpp (195f1) 1I209

L an a I 1 I( I) _i 109C

7 thinbaton al LJ Ec-ktt JkrCtem Soc (1912) 94 825d

C i 1 2 a nd Ling JCS

- 29 shy

9 MJBHqluy 2Cruubju 1J lam andc LAWhj t iio JCSChumI

(012 (i12) 1

IU iil 12 ~cit I ~ J03 lnuut Ilivio ( Ijibt) 14 106 3

11 V--1 lni I1~~u Ji)141 L)ad4 -i

1 Al~Kuai NMzkhhi ACuizwk iiI sjuriai Ayr hiil Chem (073) 1y(09

3 K Wda Y Li ato aind FKmnaat Acjr Iiol ChUri 0) (9 34 941

14 EWaija Y Lnuotu and K151Maiio Ag~r ia l Chem (1970) 14 94b

IKatc andu Yjluzaka ta Tet rahedron Le tter (19 74)

17 i v i v a n a d9

(1175) 2 197S

19 KLO2) 1 Miu-a anO C 1iako2j hi 3 CLrCcd(97 6701

20 Iamp Lbo LY-0I KNaLkaishi and ACiiapya JCS

2 1 I ibo SNpjgti Y- Mi ura Nakanislii and AChay

22 Kul~ 1 -4I ( 1 Pet tLe I 1ijwicz and~t~nrih

J C L Cnc1 Coo ) 10 1

23 1 F aboampi F Ha an i sh i T I 1 soh and CillWKa k aW TKuuta

24 7 Iedil Hout- Plan~rt CCC 2 C t-dice to Io it A C S Sympos i Utn

Meriv Uc2 Ameiricajn ChenicoI a00201 gt4 WaiinqLyofl DC (1977)

1 73

PAeIini Plaiin~t JtIC1tacc to l(2tsi CS Stpi r

Series~ U inri can Chemical SOcact washington DC (1977) 374

0

- 30 shy

26 Il Slurkil1 A Dictionary of the Economic Products of the

Hal ay enin-iula 2 VolMs Minitry of Agriculture and Cooprratives

huj ] Lupuira L-urpir (11)(6)

27 H Grair g t i]i I]ant Spc ie Prportedl y Possesing

I(rt-Control Propert ji-An IICUll )atabjaIe Vesource Systems

1nntjtitt irt-~e~ -tCrnter Hn)roluilu (1985)

23 P1 Tuntiwrwh tt ikurl -t al ht chremjftry (1981 )20 1164

29 T Imatayakul -t 11 A t Chrem (1979) 2 71

30 H HitnlI nll j Yrcwoto) Na tura llv Occurring Inecticides ed

7aconon H ann] Croby P( Marcel Dekker New York (1971) 62

31 W Itwers tatural Productf for Innovative Pest Management

ed Whitehead D L and flowers WS Pergqanon Press Oxford

(1903) 52

- 28 shy

RRIEAwaO PLAN

It can be ccn Iuded from Ihe ( iermenLs on Agtaia sp

that thre exI nt a pa qihilIty o usin this plant as an antifeedant

for Srdopten Ulna in agriculture IPlans have been node for the

I 1l1itu i IL

1) Furdther asSess other irrnu latiorn of Agaa 6p in the

field

2) Compare the activity of scopoletin which is the active

antifeedant in thi plant with other analogues

Toward the end of this reporting period we had reassesed

the insecticidol activitv of the plants which had been pieviously

studied and recorded in our earlior report with various forul ations

Pr arable roult r obtained from the prolictinary studies

0 finl period of this project will then be also spent in evaluating

their Tplical ility ira the fields

IlFFpp[ cFE

I Sai Tikwattanit Mc Thesis Kasetsart University(1977)

2Vciha Patuoumotat lSc TIhILis aseLtL rt University

Si172)

5 Rtnolh I a I I h1 Ich~z br t~nt XIAIpp (195f1) 1I209

L an a I 1 I( I) _i 109C

7 thinbaton al LJ Ec-ktt JkrCtem Soc (1912) 94 825d

C i 1 2 a nd Ling JCS

- 29 shy

9 MJBHqluy 2Cruubju 1J lam andc LAWhj t iio JCSChumI

(012 (i12) 1

IU iil 12 ~cit I ~ J03 lnuut Ilivio ( Ijibt) 14 106 3

11 V--1 lni I1~~u Ji)141 L)ad4 -i

1 Al~Kuai NMzkhhi ACuizwk iiI sjuriai Ayr hiil Chem (073) 1y(09

3 K Wda Y Li ato aind FKmnaat Acjr Iiol ChUri 0) (9 34 941

14 EWaija Y Lnuotu and K151Maiio Ag~r ia l Chem (1970) 14 94b

IKatc andu Yjluzaka ta Tet rahedron Le tter (19 74)

17 i v i v a n a d9

(1175) 2 197S

19 KLO2) 1 Miu-a anO C 1iako2j hi 3 CLrCcd(97 6701

20 Iamp Lbo LY-0I KNaLkaishi and ACiiapya JCS

2 1 I ibo SNpjgti Y- Mi ura Nakanislii and AChay

22 Kul~ 1 -4I ( 1 Pet tLe I 1ijwicz and~t~nrih

J C L Cnc1 Coo ) 10 1

23 1 F aboampi F Ha an i sh i T I 1 soh and CillWKa k aW TKuuta

24 7 Iedil Hout- Plan~rt CCC 2 C t-dice to Io it A C S Sympos i Utn

Meriv Uc2 Ameiricajn ChenicoI a00201 gt4 WaiinqLyofl DC (1977)

1 73

PAeIini Plaiin~t JtIC1tacc to l(2tsi CS Stpi r

Series~ U inri can Chemical SOcact washington DC (1977) 374

0

- 30 shy

26 Il Slurkil1 A Dictionary of the Economic Products of the

Hal ay enin-iula 2 VolMs Minitry of Agriculture and Cooprratives

huj ] Lupuira L-urpir (11)(6)

27 H Grair g t i]i I]ant Spc ie Prportedl y Possesing

I(rt-Control Propert ji-An IICUll )atabjaIe Vesource Systems

1nntjtitt irt-~e~ -tCrnter Hn)roluilu (1985)

23 P1 Tuntiwrwh tt ikurl -t al ht chremjftry (1981 )20 1164

29 T Imatayakul -t 11 A t Chrem (1979) 2 71

30 H HitnlI nll j Yrcwoto) Na tura llv Occurring Inecticides ed

7aconon H ann] Croby P( Marcel Dekker New York (1971) 62

31 W Itwers tatural Productf for Innovative Pest Management

ed Whitehead D L and flowers WS Pergqanon Press Oxford

(1903) 52

- 29 shy

9 MJBHqluy 2Cruubju 1J lam andc LAWhj t iio JCSChumI

(012 (i12) 1

IU iil 12 ~cit I ~ J03 lnuut Ilivio ( Ijibt) 14 106 3

11 V--1 lni I1~~u Ji)141 L)ad4 -i

1 Al~Kuai NMzkhhi ACuizwk iiI sjuriai Ayr hiil Chem (073) 1y(09

3 K Wda Y Li ato aind FKmnaat Acjr Iiol ChUri 0) (9 34 941

14 EWaija Y Lnuotu and K151Maiio Ag~r ia l Chem (1970) 14 94b

IKatc andu Yjluzaka ta Tet rahedron Le tter (19 74)

17 i v i v a n a d9

(1175) 2 197S

19 KLO2) 1 Miu-a anO C 1iako2j hi 3 CLrCcd(97 6701

20 Iamp Lbo LY-0I KNaLkaishi and ACiiapya JCS

2 1 I ibo SNpjgti Y- Mi ura Nakanislii and AChay

22 Kul~ 1 -4I ( 1 Pet tLe I 1ijwicz and~t~nrih

J C L Cnc1 Coo ) 10 1

23 1 F aboampi F Ha an i sh i T I 1 soh and CillWKa k aW TKuuta

24 7 Iedil Hout- Plan~rt CCC 2 C t-dice to Io it A C S Sympos i Utn

Meriv Uc2 Ameiricajn ChenicoI a00201 gt4 WaiinqLyofl DC (1977)

1 73

PAeIini Plaiin~t JtIC1tacc to l(2tsi CS Stpi r

Series~ U inri can Chemical SOcact washington DC (1977) 374

0

- 30 shy

26 Il Slurkil1 A Dictionary of the Economic Products of the

Hal ay enin-iula 2 VolMs Minitry of Agriculture and Cooprratives

huj ] Lupuira L-urpir (11)(6)

27 H Grair g t i]i I]ant Spc ie Prportedl y Possesing

I(rt-Control Propert ji-An IICUll )atabjaIe Vesource Systems

1nntjtitt irt-~e~ -tCrnter Hn)roluilu (1985)

23 P1 Tuntiwrwh tt ikurl -t al ht chremjftry (1981 )20 1164

29 T Imatayakul -t 11 A t Chrem (1979) 2 71

30 H HitnlI nll j Yrcwoto) Na tura llv Occurring Inecticides ed

7aconon H ann] Croby P( Marcel Dekker New York (1971) 62

31 W Itwers tatural Productf for Innovative Pest Management

ed Whitehead D L and flowers WS Pergqanon Press Oxford

(1903) 52

- 30 shy

26 Il Slurkil1 A Dictionary of the Economic Products of the

Hal ay enin-iula 2 VolMs Minitry of Agriculture and Cooprratives

huj ] Lupuira L-urpir (11)(6)

27 H Grair g t i]i I]ant Spc ie Prportedl y Possesing

I(rt-Control Propert ji-An IICUll )atabjaIe Vesource Systems

1nntjtitt irt-~e~ -tCrnter Hn)roluilu (1985)

23 P1 Tuntiwrwh tt ikurl -t al ht chremjftry (1981 )20 1164

29 T Imatayakul -t 11 A t Chrem (1979) 2 71

30 H HitnlI nll j Yrcwoto) Na tura llv Occurring Inecticides ed

7aconon H ann] Croby P( Marcel Dekker New York (1971) 62

31 W Itwers tatural Productf for Innovative Pest Management

ed Whitehead D L and flowers WS Pergqanon Press Oxford

(1903) 52