PART C: FINAL TECHNICAL ACTIVITY REPORT · Sciences, David Agmashenebeli Alley, 10th Km, PO Box 59, 0159 Tbilisi, Georgia Partner no 22 Mr Giorgi Nakhutsrishvili Institute of Botany,

AGRI GEN RES 0.. - Acronym: …. AGRI-2006-…. Part C I/123

PARTC:FINALTECHNICALACTIVITYREPORTReporting period from 01/01/2007 to 31/12/2010

Version 2 of march 15th 2012

Protocol of modifications and up-dates

Versions

Modifications Issued

Version 1 August 15th 2011 Version 2 Based on experts'recommandations March 15h 2012

AGRI GEN RES 0.. - Acronym: …. AGRI-2006-…. Part C II/123

AGRI GEN RES 0008 - Acronym: Grapegen06 AGRI-2006 Part C: 3/123

COUNCIL REGULATION (EC) N° 870/2004 of 26 April 2004 Establishing a Community programme on the conservation, characterisation, collection and utilisation of genetic resources in agriculture

AGRI GEN RES 008

Acronym: Grapegen06 Action title: Management & Conservation of Grapevine Genetic Resources

FINAL TECHNICAL ACTIVITY REPORT Reporting period from 01/01/2007 to 31/12/2010

Action starting date: 01/01/2007

Action closure date: 31/ 12/2010

Action duration (in months) 48 months

Total budget 1.527.216 €

EC contribution: 585.178€

(%) of total costs 38.32 %

(%) of eligible costs 45.79 %

Data Coordinator

Name of the coordinator (Partner no 00)

Administrative: L. Bruckler, President of Montpellier INRA centre

Scientifique: Patrice This

Management: Roberto Bacilieri

Contact person R. Bacilieri

Postal address

2, Place P. Viala, F- 34060 Montpellier, France

Telephone 00.33.4.99.61.22.92 / 00.33.4.99.61.22.53

Fax: 00.33.99.61.20.64

E-mail [email protected] / [email protected]

Action Website http://www.montpellier.inra.fr/grapegen06/accueil.php

Data Co-beneficiaries (final and definitive partner numbers )

Partner no 01

Mrs Erika Maul

Julius Kühn-Institut Bundesforschungsinstitut für Kulturpflanzen (JKI)

Institut für Rebenzüchtung, Geilweilerhof (BAZ-IRZ)

76833 Siebeldingen, Germany

Partner no 02

Mr Ferdinand Regner

Höhere Bundeslehranstalt und Bundesamt für Wein- und Obstbau Klosterneuburg (HBLAuBA), Wiener Str. 74, 3400 Klosterneuburg, Austria

Partner no 03

Mr Felix Cabello

Instituto Madrileno de Investigacion y Desarrollo Rural Agrario Y Alimentario (IMIDRA), Finca "El Encin" Nll km 38,200, Apdo 127, 28800 Alcala de Henares, Spain

Partner no 04

Mrs Dora Pitsoli

National Agricultureal Research Foundation (NAGREF), Egialias 19 and Chalepa str, Maroussi Athens, Greece


Partner no.05

Mr José Eiras-Dias

Estacao Vitivinicola Nacional (EVN – INIAP), Quinta de Almoinha, 2565-191 Dois Portos, Portugal

Partner no 06

Mrs Manna CRESPAN (in the place of Dr. Angelo Costacurta):

CRA – Centro di Ricerca per la Viticoltura (in the place of the old name : Istituto Sperimentale per la viticoltura (CRA-ISV)), Viale XXVIII Aprile, 26, 31015 Conegliano (TV), Italy

Partner no.07

Mrs Anna Schneider

Consiglio Nazionale delle Ricerche, Istituto di Virologia Vegetale (CNR-IVV), Strada delle Cacce 73, 10135 Torino, Italy

Partner no 08

Mrs Stella Grando

Istituto Agrario di San Michele All'Adige (IASMA), Via Mach 1, 38010 San Michele All'Adige, Italy

Partner no.09

Mr Enrico Peterlunger

Dipartimento di Scienze Agrarie e Ambientali – Università degli studi di Udine (DISAA – UNIUD), Via delle Scienze 208, 33100 Udine, Italy

Partner no 10

Mr Maurizio Boselli

Università degli Studi di Verona

Dipartimento di Biotechnology (DBT)

Villa Ottolini-Lebrecht - Via della Pieve, 70

37029 S. Floriano (VR), Italy (In the place of DOFI, University of Florence, Italy)

Partner 11

Mr Pal Kozma

FVM Szöleszeti es Boraszati Kutatointezete Pecs (FVM SZBKI Pecs), Pazmany Peter u.4., 7634 Pecs, Hungary

Partner no 12

Mrs Erzsebet Kiss

St.István University, Department of Genetics and Plant Breeding (SIU DGPB), Páter K. u.1, 2103 Gödöllö, Hungary

Partner no 13

Mr Olga Jandurova

Research Institution of Crop Production (RICP), Drnovska 507, 16106 Prague, Czech Republic

Partner no.14

Mr Laurent Audeguin

Institut Français de la Vigne (previously Etablissement National Technique pour l'Amélioration de la Viticulture (ENTAV)), Domaine de l'Espiguette, 30240 Le Grau du Roi, France

Partner no 15

Mr Savvides Savvas

Agricultural Research Institute (ARI), Athalassa, 1516 Nicosia, Cyprus

Partner no 16

Mrs Dorota Pospíšilová

Vyskumná a slachtitel'ská stanica vinárska a vinohradnicka Modra, nezisková organizácia (VSSVVM )(Research and Breeding Station for Enology and Viticulture Modra, non profit organisation), Dolná 120, 90001 Modra, Slovakia


Data Partners located outside the EU (final and definitive partner numbers)

Partner no.17 Mr Jean-Laurent Spring

AGROSCOPE-RAC, Changins, CH-1260, Nyon, Switzerland

Partner no 18

Mr Gheorghe Savin

National Institute for Viticulture and Oenology(Institutul Naţional pentru Viticultură şi Vinificaţie),

Vierul street, 59, MD-2070, Chisinau, Moldova

Partner no 19

Mr Zeynal Akparov

Genetic Resources Institute of Azerbaijan National Academy of Sciences, Azadlig ave., No: 155, AZ1106 Baku, Azerbaijan

Partner no 20 Mr Edi Maletic

Faculty of Agriculture University of Zagreb, Svetosimunska 25, 10000 Zagreb, Croatia

Partner no 21

Mr Tengiz Beridze

S.Durmishidze Institute of Biochemistry and Biotechnology Georgian Academy of Sciences, David Agmashenebeli Alley, 10th Km, PO Box 59, 0159 Tbilisi, Georgia

Partner no 22 Mr Giorgi Nakhutsrishvili

Institute of Botany, Georgian Academy of Sciences, Kojori road 1, 0105 Tbilisi Georgia

Partner no 23

Mr David Maghradze

Research Institute of Horticulture, Viticulture and Oenology

6 Marshal Gelovani Ave., 0159 Tbilisi Georgia

Partner no 24 Université Abdelamalek Essaâdi – Faculté des Sciences de Tétouan- UFR Biologie Végétale, PO Box 2121, 93000 Tetouan, Morocco


TABLE OF CONTENTS

PART C: FINAL TECHNICAL ACTIVITY REPORT .................................................................. I

1. EXECUTIVE SUMMARY ....................................................................................................... 10

� Action objectives during the reporting period ............................................... 10

� Key results achieved and outputs .................................................................. 11

2. DETAILED RESULTS ACHIEVED ....................................................................................... 12

WP 1

Work package title: Data acquisition for the SSR-marker database within the EU-Vitis database ......................................................................... 12

� Main objectives ............................................................................................. 12

� Sub-Task 1.1 : Validation of the alleles of 47 references cultivars and development of a common coding system on 9 SSR markers (6 from GenRes and 3 new) .......................................................................... 12

� Sub Task 1.2: Microsatellite analysis of chosen accessions......................... 13

� Sub Task 1.3: Encoding of data (already available and new). ...................... 13

� Sub Task 1.4.: Coordinated microsatellite analysis as needed within WP5 .................................................................................................... 15

� Conclusions ................................................................................................... 15

WP 2

Acquisition of primary and secondary descriptor data of old, endangered and autochthonous grape varieties................................................................. 17

� Main objectives ............................................................................................. 17

� Sub Task 2.1 : Recording of 48 primary and secondary descriptors analogous to Genres CT96 No81. Utilisation of the descriptors recently harmonized by UPOV, IPGRI and OIV .......................................... 17

� Sub Task 2.2: Realisation of the pictures of the cultivars ............................ 18

� Sub Task 2.3: Characterization of sanitary status of the cultivars ................ 19

� Sub Task 2.4 : Preparation of the catalogue of autochtonous cultivars ......................................................................................................... 20

� Conclusions ................................................................................................... 20

WP 3

Work package title: On-farm evaluation of agronomic features of autochthonous grape varieties ....................................................................... 21

� Main objectives ............................................................................................. 21

� Sub Task 3.1 : Identification and characterization of suitable old vineyards : identification of sites, growers, cultivars, Verification of cultivar identity if needed (in relation w/ WP1 and WP2,….) .................. 21


� Sub Task 3.2: Evaluation of agronomic features & wine quality ................ 22

� Sub Task 3.3: Evaluation of sanitary features and selection of agronomically-interesting virus-free grape varieties / accessions ................. 23

� Sub Task 3.4.: Information to growers by publication of activities and outcome in wine growers magazines ...................................................... 23

� Conclusions ................................................................................................... 24

WP 4

Work package title: Study, identification and evaluation of Vitis sylvestris germplasm ..................................................................................................... 24

� Main objectives ............................................................................................. 24

� Sub Task 4.1 : Inventory of material (including evaluation of preservation status) ........................................................................................ 24

� Sub Task 4.2: Evaluation of morphological and/or agronomic features .......................................................................................................... 25

� Sub Task 4.3: Characterization of Vitis sylvestris gene pool by microsatellite analysis ................................................................................... 25

� Conclusions ................................................................................................... 27

WP 5

Work package title: True-to-type analysis .................................................................. 28

� Main objectives ............................................................................................. 28

� sub Task 5.1 and 5.2 : Searching and verifying synonyms & homonyms . ................................................................................................... 28

� Sub Task 5.3: Solving questionable varieties ................................................ 30

� Conclusions ................................................................................................... 32

WP 6

Work package title: Database improvement and enrichment ...................................... 33

� Main objectives ............................................................................................. 33

Sub Task 6.1 : Updating of existing data in the database (update of passport data using MCPD system) and entering passport data for the new partners from Eastern Europe .......................................................... 33

� Sub Task 6.2: Improvement of the database structures and utilities (sorting features, identification of synonyms from coded SSR data,…..) ........................................................................................................ 34

� Sub Task 6.3: Transfer of new data in database (SSR, primary, secondary data and pictures, information on on-farm sites, data for V. sylvestris sites and individuals including SSR) ........................................ 36

� Conclusions ................................................................................................... 37

WP 7

Work package title: Elaboration of a long term conservation strategy ....................... 38

� Main objectives ............................................................................................. 38


� Sub Task 7.1 : Development of cryo-conservation techniques and collections ...................................................................................................... 38

� Sub Task 7.2: Introduction of material in field collection (including safety duplication) or on-farm ..................................................... 41

� Sub Task 7.3: Development of in-situ conservation procedures ................... 41

� Sub Task 7.4.: Development of a management plan (2009-2019) for Vitis resources ......................................................................................... 42

� Sub Task 7.5.: Coordination of the project .................................................. 42

� Conclusions ................................................................................................... 43

4. SUMMARY OF TECHNICAL PROBLEM(S) ENCOUNTERED (IF ANY) ........................ 47

5. SUMMARY OF THE MANAGEMENT OF THE ACTION ................................................... 48

� Meetings, workshops and seminars organised by the coordinator and the partners ........................................................................................................................ 48

� Changes in the action's management structure (If any) ............................................... 50

� Distribution of the labor in the project ........................................................................ 53

6. SUMMARY OF THE DISSEMINATION OF ALL THE RESULTS ..................................... 54

7. OVERVIEW OF ALL DELIVERABLES FROM THE PROJECT ......................................... 61

8. OVERVIEW OF ALL MILESTONES FOR THE PROJECT ................................................. 64

9. ANNEXES ................................................................................................................................ 67

Annex 0 : Results of the coding for the 9 markers. ............................................................... 68

Annex 1: SSR-marker data done within the project for WP1, Wp4 and WP5 (by July 2011) .................................................................................................................... 69

Annex 2 – WP1. Methodology and concepts for data analysis ............................................. 71

Annex 3: Summary of the state of advancement for morphological data ............................. 73

Annex 4: List of the cultivars selected by the 12 partners participating at the WP3. ............................................................................................................................ 77

Annex 5: Screenshots for 2 cultivars analysed within WP3, showing the data recorded for these 2 cultivars, in particular the data on choice of the cultivar, historical data and area global surface. ......................................................... 80

Annex 6: Example of agronomical data for several cultivars ............................................... 83

Annex 7: Example of wine data for several cultivars ........................................................... 84

Annex 8 : Frequencies of each alleles at each of the 9 locus for both the cultivars (CV) and the wild individuals (Lamb) ........................................................................ 85

Annex 9: Multivariate analysis of the samples. Analysis based on the 9 SSR: From the 2772 unique genotype, 2239 with no missing data were selected and analysed (in red the cultivars, in bleu the wild individuals). ................................ 86

Annex 10 - WP5 The contribution of partners and the results of the analysis of "questionables" varieties. ............................................................................................ 87

Annex 11 - WP 5 : What is “trueness to type” and how the concept is developed inside the European Vitis Database? ........................................................................... 88

Annex 12 – WP5 Guidelines for the compilation of MCPD descriptors related to “trueness to type” ........................................................................................................ 89


Annex13: list of the synonyms observed in the collections to be controlled ....................... 91

Annex 15 – WP5 ................................................................................................................. 107

Annex 16 : European Vitis Database: imported accessions by collections and number of described accessions by categories for the GrapeGen06 partners .......... 107

Annex 17 : European Vitis Database: imported accessions by collections and number of described accessions by categories for the non GrapeGen06 partners. .................................................................................................................... 107

Annex 18 : Listing of (1) downloadable explanations on descriptor recording and (2) excel file formats for all categories to be found on the European Vitis Database homepage. .................................................................................................. 112

Annex 19: European Vitis Database: content and data retrieval ......................................... 113

Annex 20 :WP7.3. Letter to local authorities with competence on the identified wild grapevine populations ....................................................................................... 114

Annex 21–List of the material exchanged during the 4 years of the project...................... 114

Annex 22 : List of the wild accessions introduced in the collections ................................. 119

Annex 23: Management plan for Vitis resources (2012-2019) ......................................... 119

AGRI GEN RES 0008 - Acronym: Grapegen06 AGRI-2006- Part C 10/123

1.EXECUTIVESUMMARY

Action objectives during the reporting period

The main objective of GrapeGen06 was to contribute to the successful long-term preservation of the Vitis genetic resources for the use of future generations. By ensuring an optimized characterization of these resources, we should promote their use and ensure the competitiveness and robustness of the European wine sector. The main aims of the project were subdivided into several technical objectives. Objective 1: Monitor still existing Vitis germplasm in Europe, including Eastern countries The project objectives were to record the genetic resources in Europe existing in collections, in natura for wild individuals and to present the on-farm work done within the project.

For cultivated material maintained in collections: the detailed objectives were to enter passport data for the 23 partners of the project and the new 6 external partners that joined the community during the project duration in order to present the sum of material present in the collections

For old autochthonous cultivars in on-farm conservation: we planed to present the material conserved on the several on-farm experiments in progress within the project

For wild material in-situ:: the detailed objectives were realize a surveys iin order to identify the individuals still existing in wild conditions in the different countries of the partners and communicate about the importance of the wild individuals and populations with structures of the local territory

Objective 2: Describe this germplasm, using common morphological, agronomic, and molecular descriptors and linking them to a unique web-database (www.eu-vitis.de). For this objective it is important not only to have a common set of measures applied uniformly around Europe, so to allow comparisons, but the quality of the data is also quite important. In regards to this objective, we intended to analyze part of the germplasm present in the collections using common morphological, agronomic, and molecular descriptors of high quality and to transfert this data in the European web-based database. A particular interest was also given to the quality of the data. Objective 3: Implement true-to-type identification of varieties decreasing the number of errors, homonyms and synonyms affecting the European collections In order to estimate and publish a precise online catalogue of the European genetic resources available in the different germplasm collections, we had to ensure that the possible homonyms or synonyms, misnamers or questionable accessions in the collections are identified and compared. In order to complete this objective, morphological and molecular data were performed for questionable cultivars and the data for this questionable was compare to the data from the partners databases. Comparison of the molecular data recorded in the European database enabled also the identification of redundant cultivars and of synonyms and homonyms.


Action objectives during the reporting period (suite)

Objective 4: Promote an overall optimized conservation scheme for the safe conservation of the Vitis germplasm including Vitis sylvestris currently threatened of extinction, and involving several conservation means (ex-situ collections, cryopreservation and on-farm conservation by farmers and professionals) To reach this objective, research on alternative conservation means was conducted in particular to develop cryoconservation methods. As conclusion of the project, a plan for conservation of grape genetic resources will be drawn.

Objective 5: Ensure the overall accessibility of the information by diverse media (scientific papers, technical papers, general public publications, websites, online database..) and ensure the availability of identified material for use by European growers

.

Key results achieved and outputs

Results and outputs of the projects will be given according to the general objectives presented previously. Individual contributions of the partner were not added to this document but will be available on request from the coordinators. In relation with Objective 1 , Monitor still existing Vitis germplasm in Europe Result 1: Out of the 25.583 accessions present in the partners collections, 4.323 accessions were analysed by SSR markers while 1.515 accessions have been characterized by molecular markers. Result 2: Identification of 218 populations of V. sylvestris and 1.854 individuals. Follow-up, measurement, characterisation of 52 old autochthonous varieties on farm. Result 3: Presentation of the 113 cultivars in on-farm trials. In relation with Objective 2: Describe this germplasm Result 3: Full analysis of the genotyping of 3.721 accessions within WP1 and of descriptor data for 788 accessions within WP2 Result 4: Identification in wild conditions and analysis of 631 V. sylvestris accessions for morphological data and of 269 accessions by SSR Result 5: On-farm analysis for 59 cultivars including estimation of their sanitary status Result 6: 3.678 pictures made available on the EU database for cultivars In relation with Objective 3: Implement true-to-type identification Result 7: Analysis by SRR, morphological and historical comparison, of a set of 450 “questionable” varieties submitted by 14 partners from all around Europe. Successful true-to-type identification of 177 of them, and proposition of a name for 66 additional unknown cultivars. Result 8: Comparison of these questionables to the database of the partners to see if they are unique or present elsewhere. Result 9: Use of SSR data from both WP1 (3.721 acc.) et WP5 (333) to identify synonyms or errors. A set of 2.105 unique genotype and a list of synonyms were realized. In relation with Objective 4: Promote an overall optimized conservation scheme Result 10: Positive results presented for the development of protocol for dormant bug cryo-conservation. Result 11: Seed and pollen already conserved in cryoconservation In relation with Objective 5: Ensure the overall accessibility of the information Result 12: Opening of the web database to the public; articles in scientific and vulgarisation journals, as well as presentations in scientific meeting or agro-enological fairs.

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P0 P1 P2 P3 P4 P5 P6 P7 P8

P9 P11 P12 P13 P14 P16 P20 P23 P24 Start date: January 07 The Partners involved in WP1 agreed to provide during the length of the project molecular data at 9 SSR loci for a defined number of cultivars (approximately 2,800, see EU-Vitis database). The vast majority of WP1 participating partners during the four years completed genotyping at 9 SSR loci in their own lab. P4 and P24 were supported with facilities and expertise in genotyping by partners P14 and P0, respectively. In the same way P23 was supported by an external accompanying partner (University of Milan, Italy, Ita045) who provided partial SSR profiles for 160 Georgian cultivars, completed by P00, as well as 19 Italian cultivar profiles. The total number of SSR profiles in the European Vitis Database (EVDB) is today of 3.721. 3170 were new ones produced during Grapegen06 project added to the one produced during the previous Genres81 project (Annex 1). The data was produced progressively, 10 % being produced the first year, up to 25% (872) the second and up to 82 % the third year (2.615 profiles) and the data was finished during the last year to reach the number of data of 3.170. The total number of accessions genotyped through the WP1 and uploaded in the Vitis DB was thus larger than initially planned (2.765 for the whole project including WP1, WP4 and WP5)). This was partly achieved by the contribution of external accompanying partners: C. D’Onofrio, S. Imazio (Italy); A. Jung (Germany); J. Patkova (Slovakia) who also contributed to the database with previously not genotyped accessions from their countries and/or giving the expertise. It was also the result of reallocation of work between partners. The quality of data was improved during the last year (filling missing data, solving ambiguous alleles, replicating analysis for uncertain genotypes). For example missing data represents now less than 1.4% of the total data. The number of SSR data is still growing in spite of the lengthy procedure used to ensure their global quality (extraction and distribution of reference DNAs, SSR fingerprinting across different labs, reading, coding, quality check, re-analysing missing or wrong data, etc.). Since very recently, new accompanying partners from Slovenia and Bulgaria were willing to join and contribute the database with their data and be granted with the “partner access status”. It seems that the main goal is achieved and wider scientific community recognized the consortium’s results and methodology as a very useful mean in grape germplasm management. Conclusions : Within sub-task 1.2, despite problems encountered by several EU partners and the low participation of partners from Eastern countries, relocation of work within EU partners and addition of new partners during the project enabled the realisation of more than the expected number of cultivars.

Sub Task 1.3: Encoding of data (already available and new).

Start date: January 07 No of participating partner(s) P0 P1 P2 P3 P4 P5 P6 P7 P8 P9 P11 P12 P13 P14 P16 P20 P23 P24

The encoding of the data started quite slowly, the partner being unfamiliar with the coding macro. After the rapid training session during the first meeting, the situation improved. Only one partner was not able to use it (P2) and therefore developed his own system.


In parallel to task 1.2 the % of data increased during the project, from 10% the first year to 25% the second and 82 % the third year. At the end of project all the partners who generated SSR data were able to code them in the database. At present there are SSR genotypes for 3721 accessions uploaded into the VDB for WP1. The improvement of the data quality unable to analyse more carefully the data. From a set of 2901 unique profile identified at the beginning of 2010, the work done in 2010 and at the beginning of 2011 enabled the identification of 2732 non redundant accessions in each partner set of data. The comparison of the data between the partners was also possible and lead to the identification of 2105 unique profiles. A total of 985 accessions are repeated at least once. It is these profiles that were comparable between lab, with sometime inconsistency that can be due to errors. Validation of this data will nevertheless require still much work. A population analysis based on the available unique coded data produced during the project by all regular and accompanying partners at 9 microsatellite loci was performed first during year 2009 then at the end of the project. The difference between both analyses, before and after the cleaning of the data shows that as many as 18 alleles were false. The total number of alleles (Na) found within the 2106 unique accessions of this project is of 233 and thus exceeds the Na found in the 47 reference cultivars (cv-refer: 154). Table 1: statistics of the number of allele, HA, HE et PIC obtained in the sample and in the reference set.

Locus N (cv-

refer)

N (wp1)

Na (cv-refer)

Na (wp1)

N (wp1) After

cleaning

Na (wp1) After

cleaning

Ho cv-refer

Ho (wp1)

He cv-

refer

He (wp1)

PIC Val

VVMD5 47 2901 17 27 2106 23 0.894 0.85 0.91 0.86 0.85

VVMD7 47 2901 17 25 2106 24 0.809 0.79 0.86 0.80 0.77

VVMD25 47 2901 14 25 2106 24 0.851 0.79 0.85 0.84 0.82

VVMD27 47 2901 23 30 2106 22 0.979 0.82 0.90 0.85 0.84

VVMD28 47 2901 18 34 2106 26 0.979 0.82 0.92 0.85 0.83

VVMD32 47 2901 14 25 2106 33 0.511 0.86 0.88 0.90 0.89

VVS2 47 2901 19 22 2106 20 0.894 0.81 0.89 0.83 0.81

VrZAG62 47 2901 19 25 2106 23 0.872 0.84 0.89 0.85 0.83

VrZAG79 47 2901 13 20 2106 20 0.787 0.83 ? 0.88 0.87

154 233 215 0.842 0.822 0.887 0.85 0.834

Table 2: The most frequent alleles among the 2106 profiles

Locus Coded size Frequence Partner 20

size Partner 0

size

VVMD7 N+8 29.59 237 239

VVMD32 N+37 29.19 272 271

VVS2 N+10 28.73 131 131

VVMD25 N+6 25.42 239 240


VVMD25 N+20 25.40 253 254

VrZAG79 N+12 25.04 248 250

VrZAG62 N+14 24.58 187 188

VVMD5 N+4 23.28 222 223 Allele frequency was also calculated for each allele found within the population. The 10 most abundant alleles accounting for more than 20% of the alleles of one locus are presented in Table 2. 26 alleles have frequencies ranging from 10 to 20% while 64 alleles have frequencies ranging from 1 to 10%. Finally 117 alleles are present in less than 20 genotypes, many in only 1 or 2 genotypes. These alleles are rare ones. It is evidence that the data set from this project greatly widens the knowledge on gene and genotype diversity in grapevine, as well as evidences of a successful conservation of rare genotypes. Conclusions : The coding of all data available in the data base (4323cultivars) was performed with this project. It enabled an easy comparison of data between partners.Carefull checking of the data was nevertheless still necessary (18 wrong alleles) and lead to a database of 4323 SSR profiles. Because of this system, we were able to compare the whole set of profile. The 4323 profiles obtained within the project correspond to 2105 unique profiles (see WP5).

Sub Task 1.4.: Coordinated microsatellite analysis as needed within WP5

Start date: January 07

No of participating partner(s) P0 P1 P2 P3 P4 P5 P6 P7 P8

P9 P11 P12 P13 P14 P16 P20 P23 P24

During the 4 years of the project, a total of 333 profiles were produced in order to help in the identification of unknown cultivars: 209 were produced during 2009 and 124 in 2010. They were coded using the same references and compared to the Vitis database as well as the database of each participating partners. Detailed analysis of this data is presented in WP5. Conclusions: The profiles demanded by WP5 for the identification of questionable cultivar was done and this data was coded, which enabled it’s comparison with the data in the European database and each partners databases.

Conclusions

The ultimate goal of the WP1 was to identify the non-redundant European germplasm list, publish it in the Vitis database, but also to create a network of partners that well understood each other and share common practice in grape cultivar characterization. A very big step towards this goal was achieved within GrapeGen06 project. Consortium members succeeded to join SSR data produced in many different labs and used them to discover plenty of new relationships among their germplasm repositories. In collaboration with other research groups (WP2-WP7) hundreds of previously not characterized grape cultivars will become “visible” for researchers and breeders through Vitis DB. This network is now in big part involved in the COST action FA1003. Also, we expect that the GrapeGen06 project will have a tremendous impact on the future Vitis


germplasm repositories through the standardization of several crucial procedures in germplasm characterization. After full data analysis and inventory of number of unique vs. duplicated accessions it will be possible to make a comprehensive plan of grape cultivars conservation. This project paved the road for creation of European network of Vitis germplasm repositories which has the capacity of most effective (rational and competent) germplasm preservation. We can also give several final remarks on the WP1 idea to create a large SSR data set to be used for routine search of genetically identical or related genotypes among different European institutions:

- In spite of using referent cultivars and coding system, the data still needs controls and edition before validation and further use. By careful data observation and comparison of similar data from different labs, numerous mistakes have been detected and neutralized. Human mistake was the primary source of unreliable data, but differences in protocols, lab equipment and chemicals were substantial too. Consequently, coordinators concluded it would be wise to introduce “data validation” before Vitis DB make them available for open access;

- Before publishing WP1 results it would be important to thoroughly describe “best practice of SSR genotyping”. In spite of generally well known protocols, the huge experience from this and similar projects might help to future genotyping work and data harmonization;

- As 79 new alleles have been identified, a new set of referent cultivars (minimal number of well-known cultivars presenting all possible alleles at the 9 used SSR loci has to be selected and recommended. In addition to this, it would be nice to ensure their availability (as freeze dried leaf tissue free of charge) for scientific community. This will constitute one of the content of the publication in preparation.

Moreover, having on mind some problems with tissue or DNA distribution among labs this research might serve as an input for creation of the specific locus marker ladders that could be commercially available.


Work package number WP 2

Start date.: January 07..... Month 1

Acquisition of primary and secondary descriptor data of old, endangered and autochthonous grape varieties

No of lead partner P3 No of participating partner(s) P0 P1. P2 P3 P5 P6 P7 P8 P9 P11 P13. P15 P16 P20 P22 P23

Main objectives

Global objective of WP2 : Analysis of autochthonous varieties for primary and secondary descriptors and for sanitary status. Main emphasis is laid on germplasm of Eastern European countries.

Objective 1 : Standardized description of old autochthonous varieties with special emphasis on germplasm of Eastern European countries

Objective 2 : Analysis of sanitary status of autochthonous varieties

Objective 3: Dissemination of data

Sub Task 1 : Recording of 48 primary and secondary descriptors analogous to Genres CT96 No81. Utilisation of the descriptors recently harmonized by UPOV, IPGRI and OIV Sub Task 2 : Realisation of the pictures of the cultivars

Sub Task 3: Characterization of sanitary status of the cultivars

Sub Task 4 : Preparation of the catalogue of autochtonous cultivars

Sub Task 2.1 : Recording of 48 primary and secondary descriptors analogous to Genres CT96 No81. Utilisation of the descriptors recently harmonized by UPOV, IPGRI and OIV

Start date...... No of participating partner(s) P0 P1. P2 P3 P5 P6 P7 P8 P9 P11 P13. P15 P16 P20 P22 P23 During the first year of the project, the list of old endangered and autochthonous cultivars that will be studied has been elaborated as well as the updated list of I and II descriptor codes (OIV-IPGRI). A model format for facilitating data circulation and submission by each partner to the database was also prepared.. 48 descriptors were selected to be used in the GrapeGen06 project. More than 1/4th of the data were collected (full description of 200 cvs out of 700). During 2008, a total of 490 cultivars have been described or redescribed which bring the number of varieties described to a total of 610. A training course was held during the Gödollo meeting. The teaching was performed by 5 partners. It was attended by 22 persons, partners present in Gödollo. During 2009, 2 types of works were performed:

- WP2 leader provided a summary table of all the wrong notations registered during the two previous years, according to the guidelines adopted in the project. These data were verified.

- In addition, data for the 48 morphological descriptors for all the autochthonous accessions and the Vitis vinifera subsp. sylvestris accessions, were uploaded into the European Vitis Database thanks to the powerful online tool built by WP6 leader.

During 2010,the work was then finished in order to reach the description of 788 accessions for WP2 (some being common between WP2, WP5). Every partner was in charge of uploading its ampelographic data into the European Vitis Database. This was possible thanks to the online tool built by WP6 leader (Partner 1) that can be used for uploading the 48 morphological descriptors for all the autochthonous accessions (WP2 and WP3), questionable varieties (WP5) and the Vitis vinifera subsp. Sylvestris (WP4).


Conclusions : In total, within the project, 955 morphological descriptions were performed by the partners involved in this sub-task (the work with the one performed withinWP4 and WP5 represent almost more than twice the expected work indicated in the contract). This data will be usefull in order to enable the realisation of the varietal sheets. The lower numbers of analysis of some partners were fully compensated by the additional work done by other partners. More than expected work was thus performed within the project.

Sub Task 2.2: Realisation of the pictures of the cultivars

Start date. January 07 No of participating partner(s) P0 P1. P2 P3 P5 P6 P7 P8 P8 P9 P11 P13. P15 P16 P20 P18 P22 P23 During 2007, few pictures were taken. After discussions between partner P0, P1 and P3, a common system for labelling has been proposed, taking into account the link with the European Vitis Database.

During 2008, for easier management of the common database (WP6), the common system of label adopted at the Gödöllo meeting has been applied to name the pictures. The picture size (under 300 kb) and format (.jpg) were applied to fit to the common decision taken at the Gödöllo meeting. Picture of the cultivars have been taken for about 394 accessions. During 2009, additional pictures were taken, up to 2515 pictures. In addition, pictures were uploaded into the European Vitis Database thanks to the powerful online tool built by WP6 leader. During 2010, photos of shoot tips, leaves and clusters have been made up to 3678. The online tool built by WP6 leader (partner 1) was used for uploading the technical pictures into the European Vitis Database, for all the autochthonous accessions (WP2), questionable varieties (WP5) and the Vitis vinifera subsp. sylvestris (WP4). All the pictures were labelled according to the common system adopted in 2008, and it has been applied to name the pictures. The picture size (under 300 kb) and format (.jpg) were applied as during previous year. Most of the uploaded pictures however correspond to the autochthonous accessions since they are useful for the catalogue. Few individuals of V. sylvestris with bunches (females) were also uploaded. Conclusions: More than 3678 pictures were tacken mainly for the different autochthonous accessions. The pictures will be used for the varietal sheets.


Sub Task 2.3: Characterization of sanitary status of the cultivars

Start date January 07 No of participating partner(s) P0 P1. P2 P3 P5 P6 P7 P8 P9 P11 P13. P15 P16 P20 P22 P23 In 2007, during the workshop in Versailles, it was agreed that the minimal list of virus to be tested are Grapevine fanleaf virus (GFLV) , Grapevine leafroll-associated virus type 1 (GLRaV-1) and type 3 (GLRaV-3) using the ELISA method. Only partners 3, 12, 16 have started this sub-task by Elisa testing.

During 2008, a new decision was taken in the second workshop in Gödöllo: because of EU rules, the partners will record sanitary status of varieties according to a new virus “ArmV” (Arabis mosaic) in addition to the former ones: GFLV, GLRa1V and GLRa3V. The sanitary status of aroung 300 cvs (see annex 4) was either known or in progress (which represent about half the described material). By 2010, 467 virus testing have been performed. The data was transferred in the database thanks to the online tool built by WP6 leader (partner 1). Globally, out of the 467 accessions tested, 443 accessions gave results: 139 are exempt of the 4 viruses (see annex 3 bis for the list of the cultivars). Considering the virus individually, 71 acc. are infested with GFLV, 28 with GLRaV-1, 180 with GLRaV-3 and 39 with ArMV. Only 7 accessions are infested with the 4 viruses, 23 with 3 viruses , 76 with 2 viruses, and 208 with only one virus. If we consider that this represent the global sanitary status of the collections, only about 31 % of the accessions are free of these 4 viruses. The other ones are infested with at least one virus having an effect on the phenotype and the survival of the vines. Conclusion: This task, demonstrated that most of the material conserved in collection is of poor sanitary status. Specific actions for cleaning the grape material should thus be launched in the future in order to clean it up.


Sub Task 2.4 : Preparation of the catalogue of autochtonous cultivars

Start date...... No of participating partner(s) P0 P1 P2 P3 P5 P6 P7 P8 P9 P11 P13. P15 P16 P20 P22 P23 Within WP2, the objective of sub-task 2.4 was to prepare a catalogue of the cultivars present in the collection: Each varietal sheet should represent a synthesis of primary and secondary descriptions held within the database, including pictures. It will be of particular interest for the autochthonous cultivars. Since the catalogue is based on compiled data obtained during the first years of the project (ampelographic descriptions, pictures and phytosanitary status), it was decided to postpone the beginning of this sub-task until the third year of the project. During 2009, it was chosen to develop an online tool for editing these varietal sheets under pdf format that can be downloaded directly from the European Vitis Database.

In the objective to inform users about the most important characteristics and growing aptitudes of neglected autochthonous varieties, the most important descriptors have been chosen. High-resolution photos will be used for the printout.

The online tool is now working (see WP6) and provides access to all type of data available in the database through the public access: http://www.eu-vitis.de/index.php.

Via the query window the varieties needed by the users have to be selected. After that the best-suited photos can be chosen. A PDF document can thus be generated encompassing the most important characteristics and photos of shoots tips, leaves and bunches (original size for a better hardcopy quality).

All the minor autochthonous cultivars described during the previous European program (GenRes 081) and during the present project (GrapeGen06) can be printed. The sum of all cultivars available in the database represents the catalogue of autochthonous cultivars for each country. The catalogue does not exist as such, but is simply created at the demand: a description of any cultivars from the database can be edited using the tool. Conclusion: As expected, we were able to create a tool enabling the creation of a varietal sheet for any of the cultivar present in the database with picture if by any chance the picture exists. Many pictures were uploaded in the database, mostly for V.vinifera cultivars.

Conclusions

In conclusion, during the 4 years of the project, 788 analyses (112% of what was expected from EC countries) and 467 virus testing were performed. In total 3678 pictures have been uploaded and are available through the pdf system of printing catalogues of autochthonous. This tool enables any user to create the catalogue of autochthonous cultivar from each country and print it. In addition, it clearly add values to the field collections were the cultivars are maintained, since their accessions passport data, descriptions and collection holder addresses are now accessible on the WP6 database. Any database user (viticulturists, nurseries, breeders, researchers) can thus search for a variety of interest, identify the collection holder, and ask cuttings for local propagation and use. In many case however, this material may be of poor sanitary status, which may reduce its real dissemination to the growers.




Work package title: On-farm evaluation of agronomic features of autochthonous grape varieties

No of lead partner P10 No of participating partner(s) P01 P02 P03 P05 P06 P07 P08 P09 P10 P11 P13 P14 P15 P20

Main objectives

Global objective of WP3 : Develop a knowledge on some of the autochthonous varieties to be used by growers in EU based on field data.

Objective 1 : Improving our knowledge of agronomic and wine features of neglected varieties

Objective 2 : Promoting their availability (materials and information) to growers

Objective 3: Dissemination of data

Sub Task 3.1 : Identification and characterization of suitable old vineyards : identification of sites, growers, cultivars, Verification of cultivar identity if needed (in relation w/ WP1 and WP2,….)

Start date: January 07. No of participating partner(s) P01 P02 P03 P05 P06 P07 P08 P09 P10 P11 P13 P14 P15 P20 The search, done in 2007 by the 14 partners participating to WP3, leaded to identify 31 growers willing to cooperate, localized in 12 countries. For these, 113 cultivars and 192 presumed clones to be studied has been defined

After identification of the material, each partner realized an initial description of the cultivars growing on-farm including information about the owner (owner name, city, address) and the vineyard (geographical localization, exposure, soil type, age, planning and spacing, direction of the rows and rootstocks).

During the first year of the project, few identifications of the cultivars have been performed by ampelography.

At the beginning of 2008, a protocol for the analysis has been defined.

All partners verified the neglected and/or rare status of the autochthonous cultivars to be studied as well as the trueness to type. A detailed identification of 65 cultivars in 8 countries has been realised, principally by ampelography.

In 2009 and 2010, data concerning vineyards and grape varieties analyzed have been prepared to be easily transferred to the official European database (WP6) and facilitate the communication and dissemination of the data (WP7). The list (Annex 4) has been also put on the GrapeGen06 web site, as well as all WP3 protocols describing the different information to be supplied for the description of both cultivars and vineyards..

Data on cultivar name and synonyms, historical data, actual information (on current acreage and cultural importance), morphological descriptions and molecular profiles, as well as data describing vineyard features were updated. The selected 32 primary descriptors and 12 secondary descriptors were used to describe the ampelographic and agronomic aptitudes of varieties. .

The trueness to type of each plant has been verified when necessary by SSR-marker analysis (See annex 4). This data is now available on the database for each cultivar. The tables show also the location of the vineyards, historical references of ampelography, news and reasons for interest in these old varieties. Annex 5 present two examples of data.


Sub Task 3.2: Evaluation of agronomic features & wine quality

Start date: January 09 No of participating partner(s) P01 P02 P03 P05 P06 P07 P08 P09 P10 P11 P13 P14 P15 P20

During the first year, we defined the protocols for the evaluation of agronomic and oenological features of the cultivars. All the partners undertook the maintaining and uniformity of agronomic management during experimentation years. Some “international” cultivars were also selected as reference for several agronomic features (for example: date of harvest). During 2008, many partners realised a detailed evaluation study of the cultivars selected according to the WP3 protocol. A detailed description of agronomic features have been realised by 12 partners from 8 countries for a total of 59 cultivars. Concerning enological features, the group has also decided together to study and evaluate experimental wines obtained from at least 100 kg of grapes or from 200 plants, using a wine panel tasting. In 2009, partners involved in this WP, realised the evaluation study of the cultivars selected according to the WP3 protocol, also accomplishing a second year of evaluation as well as producing optional data interesting for better describing the studied cultivar. Detailed descriptions of agronomic features have been achieved by 12 partners from 8 countries for a total of 60 cultivars. All data have been transferred to WP6 coordinator and then data will be available on the European database. The protocol for wine evaluation methodology has been developed and it is already available on the project extranet web site (https://www1.montpellier.inra.fr/grapegen06/extranet/). Few partners have achieved the wine quality evaluation (P3, P7, P14). In 2010, the analyses were extended. Most of the partners realised the evaluation study of the cultivars selected according to the WP3 protocol, also accomplishing a fourth year of evaluation as well as producing optional data interesting for better describing the studied cultivar on farm. Detailed descriptions of agronomic features on farm have been achieved by 14 partners from 10 countries for a total of 59 cultivars. All data have been transferred to WP6 coordinator and now data are available on the European database. Annex 6 presents examples of data for 4 samples. Conclusion:

All partners have achieved the wine quality evaluation. All partners have presented the data analysis of wines, mainly technological. Only a few have presented data on anthocyanins profile of red grapes as additional data. Five partners have tasted the wines (Annex 4). Annex 7 presents examples of data for 5 samples.


Sub Task 3.3: Evaluation of sanitary features and selection of agronomically-interesting virus-free grape varieties / accessions

Start date: January 09 No of participating partner(s) P01 P02 P03 P05 P06 P07 P08 P09 P10 P11 P13 P14 P15 P20 During 2007 and 2008, The sanitary status of some accessions under analysis, have already been checked either by visual checking or by Elisa tests.

The majority of the cultivars under study have been established as virus free (GFLV, ArmV, GLRV1 and GLRV3) principally by visual checking. In 2009 & 2010, all partners evaluated the sanitary features of the selected grape varieties by Elisa tests. Sanitary (virus infection) data for cultivars are available online on the EU Vitis database. Varieties analyzed by Elisa up to date are indicated in Annex 4 . The other varieties were analyzed by visual inspection. Many varieties tested were indeed contaminated, especially by GLRaV-1. The replacement of these plants with virus free plants still requires considerable work for preselection in the vineyards. This was not planned within the present project.

Sub Task 3.4.: Information to growers by publication of activities and outcome in wine growers magazines

Start date: January 08 No of participating partner(s) P01 P02 P03 P05 P06 P07 P08 P09 P10 P11 P13 P14 P15 P20 Many historical and technical data about the cultivars have been collected by partners and they will serve as starting point for the presentation of these old autochthonous cultivars to growers. The work of identification of suitable old vineyards promoted the interest of grape-growers to autochthonous cultivars. Furthermore, the cultivar agronomic evaluation involved directly many growers allowing a first important dissemination of data concerning neglected and/or rare cultivars. All data were harmonized by the coordinators of the WP3 and WP1 and were included in the database. All data collected by partners in the research were included in the database online. Data were presented to wine growers and general public held during the tasting locally or in publications in local journals of viticultural or enological interest, as for example “Enoforum“ in Italy. Data were presented to wine growers and general public held during the tasting locally or in publications in local journals of viticultural or enological interest, as for example “Enoforum“ in Italy (Vrana & Jandurova 2010, Righetti & Boselli, 2011; Campara et al, 2011; ).

For the following partners , the tasting and dissemination events were organized :

P01: Public tasting at Weintage Südliche Weinstraße P02: research institute panel P02: research institute panel P07: research institute panel (wine experts) P10: Wine tasting with growers at Enoforum P15: Research institute panel The tasting was done using the wine evaluation sheet available on the GrapeGen06 website. The tasting was done using control wine typical of the regions of tasting or of the type of wine corresponding to the tested one (white for white, .. ).


Conclusions

In total, after the 4 years, 59 cultivars have been analyzed (see annex 4 for the list and years of analysis) and data for characterization, technical and wine characteristics are available on the website.


Start date: January 07 Month …

Work package title: Study, identification and evaluation of Vitis sylvestris germplasm

No of lead partner P05 No of participating partner(s) P00 P01 P02 P03 P05 P06 P07 P22 P23 De494

Main objectives

Global objective of WP4 : Inventory and analysis of Vitis vinifera subsp. sylvestris populations in Europe

Objective 1 : Inventory of Vitis sylvestris populations in Europe

Objective 2 : Morphological, agronomic and genetic characterization of material

Objective 3: Dissemination of data (except exact location for safety concern)

Sub Task 1 : Inventory of sites and materials (including evaluation of preservation status) Sub Task 2 : Evaluation of morphological and/or agronomic features

Sub Task 3: Characterization of Vitis sylvestris gene pool by microsatellite analysis

Sub Task 4.1 : Inventory of material (including evaluation of preservation status)

Start date...... No of participating partner(s) P00 P01 P02 P03 P05 P06 P07 P22 P23 De494

At the beginning of year one, and more specifically after the Versailles meeting, a protocol for inventories and more specifically for site and individuals description have been defined between the partner, and they all agreed on the final protocol taking into account important population characters : the number of plants in the population, the sex of these plants, the presence in the proximity of other Vitis which could intercross with the wild population, the preservation status, the risk of loss of the population, and the owner of the land (see https://www1.montpellier.inra.fr/grapegen06/extranet/menu_activities_room_index/page_wpIV/wpIV.html). During the first year, inventories were performed by 10 partners in 8 countries. More than 100 sites were identified and their positioning obtained by GPS mapping. In these sites, around 1000 plants have been identified In 2008, additional inventories were performed by 10 partners in 8 countries. 87 sites were identified (see table) and their positioning obtained by GPS mapping. In these sites, around 1 383 plants have been identified among them 615 are identified as dioecious plants. In 2009 very few extra sites were observed (2) with 57 plants observed. The total number of site was thus 189 with 1440 populations.

Finally in 2010, additional prospections were realised. They brought the number of populations to 218 and 1.864 plants have been observed. Among them 649 were identified as flowering male (315) or female (334) dioecious plants, thus confirming their wild status (the cultivated grapevines are mostly


hermaphrodites with the exception of few females).

The problem of the definition of a “population” compared to the notion of “botanical station” or “collecting site” still remains. It may lead to a heterogeneous way to record (or to group) the data between the different partners. Conclusions

In total, we have thus discovered a small number of wild plants: only 1.864 plants in 218 populations. This reflects the status of wild grapevine, which is quite rare in Europe and Africa. Furthermore, this material, often present in endangered area is itself quite endangered. Specific procedure will be proposed in the management plan in order to increase the conservation of this material.

Sub Task 4.2: Evaluation of morphological and/or agronomic features

Start date.; January 2008 No of participating partner(s) P00 P01 P02 P03 P05 P06 P07 P22 P23 De494 This sub-task only started in 2008. The morphological description is the same as for WP2 and the agronomic features are the same as for WP3. During 2008, a total of about 374 plants (see Annex 6) have been characterized for morphological and berry and bunch traits. These plants correspond to more than 71 populations from 5 countries. During 2009, data for 137 accessions of wild grapes (total of 511) were recorded. Finally, during 2010 123 extra plant have been analysed. In total during the project 631 morphological characterisations have been up-loaded in the database. In addition to planed work, 118 virus tests were realised on V. sylvestris. The methodology of the analysis is similar to the analysis done within WP2. The list of descriptors is provided on the Grapegen06 website. The unequal involvement is directly connected with the number of wild individuals found during prospecting trips. Partners 0, 1, and 3 choose to perform the morphological analysis ex-situ, while the other partners (P02, P05, P07, P08, P21, P23) perform it in-situ

Sub Task 4.3: Characterization of Vitis sylvestris gene pool by microsatellite analysis

Start date.: January 2008. No of participating partner(s) P00 P01 P02 P03 P05 P06 P07 P22 P23 De494


Similarly, the molecular analysis of the wild individuals started only during the second year. The molecular characterization is made using the same methodology as for WP1. During 2008, a total of about 150 individuals have been analyzed using the 9 SSR markers (some with about 20 markers). During 2009, 55 other individuals were analysed (total of 205 profiles), 71 of which were uploaded in the database. Finally during 2010, an additional 64 individuals were analysed. In total 269 individual were thus analysed, 112 from France, 8 from Germany, 96 from Spain, 30 from Italie and 23 from Georgia (Annex 1). Additional analyses were also realized by partner 0 on material from Slovakia. Data is presently being controlled before transfer to the data base. The 269 wild individual analysed within this sub-task correspond for P00 to the plant already introduced in the collection, easily exchangeable. For P02, a selection of 96 individuals was performed on both location in Spain and morphological data. For the other partners, the choice was at random. Analysis of the data for the lambrusques in comparison to the cultivars revealed a lower diversity in the wild sample (Table 1). Table 1 : General statistics of the analysis : number of individuals, expected and observed heterozygosity and number of alleles for the cultivars (CV) and wild (Lamb) individuals Population Sample size Loci typed Unbiased Hz Obs Hz No Alleles CV 2531 9 0.8508 +- 0.0095 0.8238+-0.0026 30.11+-7.25 Lamb 241 9 0.7309 +- 0.023 0.6282 +- 0.011 11.78 +-2.73 The wild sample indeed show much lower number of alleles and almost no specific alleles (Table 2) : the wild samples display from 6 to 15 alleles and only 3 specific alleles while the cultivars displayed between 20 and 37 alleles. Annex 8 presents the frequencies of each allele in both compartments. Table 2: Alleles revealed by the analysis: Total number of alleles, and number of alleles revealed for the cultivars (CV) and wild (Lamb) individuals. Number of alleles specific of each compartment

locus VVMD5

VVMD7

VVMD25

VVMD27

VVMD28

VVMD32

VVS2

VrZAG62

VrZAG79

nb alleles total 26 28 25 37 36 43 32 27 21 CV 26 24 25 37 36 43 32 27 20 Wild 10 15 12 11 15 12 13 6 12

nb alleles specific to

Wild 0 2 0 0 0 0 0 0 1 CV 16 13 13 26 21 31 19 21 9

When all data was compared, a set of 2772 unique profiles was defined. We performed the analysis with missing data (2772) and without (2239). In both cases, part of the wild samples clustered at the periphery of the cultivars, with some wild individuals being in the cultivated group. Further analysis


should be made to check these individuals for their wild status (see annex 9 for the analysis with the 2239 individuals). Next Steps (2011, out of the GrapeGen06 project) Partner 0. INRA (France): the 81 wild individuals collected in October 2010 will be planted in the French collection in March 2011. Then, we will extract DNA in order to perform the SSR analysis. Once the results available, we will “upload” the data into the European Vitis Database.

Conclusions

In conclusion, within WP4, 1854 wild individuals were identified in 218 populations in 11 countries, confirming their endangered status. Out of theses, we collected data and/or DNA for SSR analysis out of 649, the other being impossible to reach in most of the cases. The SSR analysis has been performed for 269 of the individuals. Analysis of the diversity and comparison with cultivars will be presented in the final report. Even if their number is small, they nevertheless represent a quite interesting genetic material. In comparison to the possible 5,000-10,000 cultivars held in the collections, this still represent a fairly good number of accessions and possible sources of valuable genes : disease resistance gene but also several abiotic stress resistance, quality traits,... Extra work should be performed on this material. A core-collection can now be defined on which extra analysis either genotyping or phenotyping should be performed.

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Updating of partners MCPD for descriptors related to “Trueness to type” During the 3° workshop held in 2009 and later, efforts were addressed to clearly explain the established rules for several passport descriptors related to “Trueness to type”, in order for all partners to properly review and upload the MCPD of their collections with special regard to true identification of materials when known. It was stated that:

the descriptor “Trueness to type”, referring to the “Variety name”, becomes “Trueness to type of the variety” and envisages the following choice options: yes, no, not checked, no reference;

the descriptor “Remarks to the accession name” indicates the trueness to type status of the accession; choice options are: dialect term, homonym, misnomer, synonym, true name;

the descriptor “Variety number VIVC” was added for retrieving synonyms among different countries, or to link, through the variety number indicated in the Vitis International Variety Catalogue (http://www.vivc.de/index.php), a same cultivar called with different names in different countries (example: Trebbiano toscano, ITA = Ugni blanc, FRA = Talia, PRT)

a set of further descriptors completing the trueness to type status were conceived, in order to indicate whether the identification of an accession with a known reference variety was achieved through:

oampelography (plant morphology)

oSSR markers (matching genetic profiles)

obibliography (historical or actual descriptions), with precise indication of the literature references

The above listed descriptors related to “Trueness to type” were incorporated in the EU-database under WP6 and their use extended and recommended during 2010.

A short document clarifying what is “trueness to type”, and how this concept was developed inside the EU-database, was prepared for project partners and further users/contributors to the database. The document will be available on-line and linked to “characterization descriptors” and “trueness to type” descriptor and in the users’ handbook. (http://www1.montpellier.inra.fr/grapegen06/technical/menu_activities_room_index/page_wpV/wpV.html) See Annex 11 & 12)

The above described action (action A) was aimed to retrieve and to disseminate information that partners, as collection curators, could achieve or had already achieved by focusing their activities on accession identification (through field surveys, bibliography, reference examination, etc.).

For accessions still remained “questionables” (unknown, un-identified or believed misnomer), action B described below was carried on. Results achieved

At the end of 2010, 36 institutions from within or outside the EU project had entered the passport data of their collections into the Eu-database. For as many as 19.299 accessions the true to type status was given. 11.278 accessions were considered true to type by collection curators, 7612 were “not checked”, and further 409 uncertain or without reference for their true to type status.

As to the Grapegen06 partners, 26.702 are the uploaded accessions, with 9.674 considered true to type varieties, and 7.788 not checked yet for their true to type status. Those indicated as true to type by collection curators include: accessions truly named (therefore corresponding to the name of the variety), synonyms, homonyms or misnomer. As an example, the accession “Acini piccoli” (number ITA360-516) is an obvious misnomer, whose the true name is “Trevisana nera” (local cultivar registered in the Italian Catalogue); the “Moscatel de grano menudo” (true name) is sometimes wrongly called Albariño in Spain (accession number ESP080-BGVCAM217), etc.

The descriptors related to “Trueness to type confirmation”, every time they could be filled up with bibliography notations, certificate the true references for the variety, essential especially for local, autochthonous cultivars, whose identity is often unknown, confused or questionable.


1. Verifying synonyms and homonyms in the database (in relation with WP1)

The SSR data available in the database were analyzed using the macro MSstool enabling to identification and listing of identical individual. The data from WP1 was analysed carefully in order to identify the unique profiles and the statistics of the presence of the accession in the collections. Out of the 3147 new profiles, the redundant profiles from each partners were removed (for example 54 accessions of Syrah, 47 accessions of Pinot N, 38 accessions of Gamay and Chardonnay and 33 accessions of Grenache noir were identified). It leads to 2721 accessions (except missing data and possible errors) from different collections. The comparison of this data identified 2105 unique genotypes corresponding to the core of the non-redundant accessions of the European germplasm. These genotypes are present either in one collection (1737) corresponding for the moment to the unique genotypes, to up to 8 collections (Pinot noir). Similarly, it also identified a first list of synonyms (Annex 13) to be confirmed and certainly extended (see task 7.4). In addition, several partners carried out the comparison of the thousands of SSR profiles provided under WP1, with the SSR database developed by their institutions, searching for matching profiles. This additional task was performed in order to improve the overall quality of data available in the EU-database for material identity and trueness to type. This work has also to be regarded as an important first test on how external users through the public access of data could compare allelic profiles of their proper accessions with the ones available on the database. Conclusions Within sub task 2 & 3 of WP5, considerable work was achieved in order to help us understand the composition of the collection. Out of 3147 SSR profiles, only 2105 unique profiles (around 66 % of the total accessions) represent unique cultivars. Extrapolation to the25 583 accessions in the collections brings the possible number of different cultivars to maximum of 16 000 cultivars in the collections.

Sub Task 5.3: Solving questionable varieties

Start date: January 2007

No of participating partner(s) P0 P01 P03 P05 P06 P07 P09 P14

P15 P16 The task consisted in the identification of a list of questionable accessions through the comparison of their genetic SSR profiles and ampelographic descriptors (morphology and photos) with reference, known varieties. In every collection are present a certain number of varieties that, in the feeling of the collection curators, are “questionable” regarding their identity. “Questionable” accessions are those clearly misnamed (i.e. “False Cabernet Sauvignon”), those without a proper name (“Unknown”), or those that, being identified in a certain area (often by local historical references), should be checked for their occurrence in other places/countries (perhaps under different names). A list of “questionable” varieties to be submitted was therefore prepared by each partners, who also carried out DNA analyses and data coding of these accessions (according to the methods established under WP1) as well as morphological descriptions and photos (according to WP2). Since the European Vitis database was still under development in the implementation of genetic profiles, and considering that mainly local, autochthonous varieties would have been uploaded, “questionables” were analyzed by comparing their allelic patterns with those included in the SSR databases developed by each partner institution, that were therefore used as reference databases. Each partner contributing to the analysis of “questionables” carried out the coding of its own SSR database (see Annex 14 for the example of partner 7 ) and the comparison of genetic profiles by means of the “macro”


application specifically realized by Partner 0 to be used in the project. During Year 2007, a list of 161 questionable was defined. By June 2008 other partners joined this sub-task and the list of questionable to be analyzed included then 212 accessions from 13 partners (0, 1, 2, 3, 5, 6, 7, 8, 9, 10, 14, 15, 16). In 2009 partners participating to the questionable work were asked to give specific information (official name in the country, acreage and growing areas, historical background) about the varieties identified in 2008. Partners were also asked to provide morphological descriptions and photos of their “questionables”, to be uploaded into the database and to corroborate identification output resulting from SSR profile comparison. Furthermore, in 2009 a second call for “questionables” was launched, asking all partners to submit another set of unknown or unidentified accessions to be analyzed. Partners agreed to send the SSR profiles (from 6 to 9 common markers) of their “questionables”, ampelographic descriptions and photos. In order to increase the chance to discover the true identity of the largest number of “questionables”, it was decided that all partners with SSR analysis facilities (owning at least a minimum set of SSR genetic profiles) should participate to the comparison of “questionable” profiles with their SSR data (data that was not necessarily included in the European database). All partners were thus requested to code their own molecular data set, according to the coding system set up under WP 1 and the “macro” available on Extranet, and be prepared to compare them with the new set of “questionables”. In order to give to the varieties still unknown from the first “questionables” call (nearly 100) a second chance to be identified, their molecular coded profiles were also included in the data set to be analysed by all partners. The new set of SSR profiles has been collected, prepared and on January 21rst 2010 sent to all partners by WP 5 leader. In total 450 “questionable” accessions from 15 partners will be analysed at this time. 177 “questionables” (40% of the submitted ones), were truly identified and correctly named. For further 66 accessions an identity hypothesis was proposed, but more investigations (more markers or more information on variety background for correct name assignment) were required. 207 accessions (46%) still remained un-identified. For the 177 identified accessions, as many as 530 were the matching allelic profiles: the identity of every “questionable” was therefore confirmed on average by 3 reference SSR profiles (from different partner databases), leading to reliable recognition.

Morphological descriptors and photos were also checked by partners in order to corroborate the output of the identification resulting from SSR profile comparison. Several examples of such morphological comparison are included in the annex 15,

All questionable accessions submitted are indicated when looking through the EU-database. The passport descriptor “Questionable” was in fact designed for aware users either about accession identity still questionables (“questionable: yes”) or about their solutions (“questionable: identified”).

Conclusions

Work on 450 questionnable cultivar has been achieved. It represents an important work which only enabled the identification of 117 cultivars (26%). The quantity of work done within sub-task 5.3 was due to the motivation of the WP leader and the willingness of the partners to perform this task.


Work package number WP 5 Start date.: January 07..... Month 1 Work package title: True-to-type analysis

Conclusions

The work carried out under WP 5 developed and experimented methods aimed to:

specify the trueness to type of the accessions held in the European collections and their correct denominations (according also to the publication of the linked Grape Cultivar Catalogues of the European and Third Party States);

develop a method for improving EU-database quality and performance about the warning of true to type status of materials, as far as contributors will acquire information through ampelography, genetics ad bibliography recognition;

develop a cooperative approach aimed to solve the identification of accessions considered “questionables” (i.e. misnomer or unknown);

specify synonyms and homonyms of the autochthonous grape cultivars held in the European collections.

The EU-database was therefore developed to acquaint users with the true to type status of every accession, as far as it becomes known to the collection curators through ampelography, bibliography or DNA fingerprinting analyses. The designed descriptors referring to trueness to type showed to be suitable for giving complete and exhaustive information on the identity of materials held in collection. The enhancement of information and documentation about true accession identity, however, is an ongoing process, progressing as far as partners (as well as further contributors to the EU-database) will be acquainted with the precise identification of their varieties and the appropriateness of their names. It is therefore expected an enhancement in the quality of the information provided by the EU-database in the near future.

The development of a reliable method for the analysis of accessions considered “questionables” by partners (i.e. misnomer or unknown), through allelic patterns comparison of true references and further evidences by morphology, has to be regarded as a remarkable cooperative approach to variety identification that could be extended to other regions (in and out of Europe) and/or applied to other crops. The results achieved, with nearly half of the problematic accessions being identified, appear significant, justifying the expectation of an improvement of the identification capacity as far as other partners will joint the action, sharing data and information on grape varieties through the EU-database. The assignment of the proper name to the identified accessions, however, still remains a crucial task, that requires different competences and specific information about variety background (such as provenance, growing areas, synonyms, homonyms, history, etc.) on both “questionable” and reference cultivar/cultivars.

The cooperation among partners or future contributors to the EU-database is regarded as essential for the enhancement of data quality, especially as material trueness to type concerns, which is regarded as a complex and delicate process. In this respect the identification of varieties hold as “questionables” by partners (i.e. un-known or surely misnomers) under Grapegen06 has to be regarded as a encouraging achievement either for the number of solved varieties (nearly half of the analyzed “questionables”) either for the methodology specifically developed.

AGRI GEN RES 0008 - Acronym: Grapegen06 AGRI-2006- Part C: 33/123



Work package title: Database improvement and enrichment

No of lead partner P01 No of participating partner(s) All Partners

Main objectives

Global objective of WP6 : Establishment of a sustainable EU-Viti-database, comprising all the data of Genres081 and the data recorded and elaborated within GrapeGen06

Objective 1 : Transformation of data already stored in the database according to new standards.

Objective 2 : Improvement of data management capacities of the database

Objective 3 : Increase the content of the database

Introduction Preservation and monitoring of genetic resources and thus networks of germplasm repositories tightly depend on documenting systems, like the European Central Crop Databases (ECCDBs). To date in all 58 ECCDBs data upload and data management has been done by ECCDB managers. With respect to the European Vitis Database in order to disburden the manager from this task and in addition to offer the curators of collections more flexibility on data maintenance and aiming to achieve a continuous follow up even after the termination of GrapeGen06 an alternative had to be found. Hence for the first time in an ECCDB an on-line uploading application and interactive modification system has been implemented.

Sub Task 6.1 : Updating of existing data in the database (update of passport data using MCPD system) and entering passport data for the new partners from Eastern Europe

As the European Vitis Database (www.eu-vitis.de) had to be newly created (see Sub Task 6.2) the alignment with the EURISCO system exigencies could be easily solved. On the contrary the transfer of the content of the previous European Vitis database, generated in the scope of Genres081 has been more complex. More precisely the accession-number, which is the primary key of the database and thus a unique identifier linking accession specific information (descriptor data, pictures, SSR-marker data and virus status), used in Genres081 had to be modified. The reason was that within Genres081 the same accession number had been assigned twice or even more often. Hence within Genres081 the correct linking of data had not always been possible. Thus the Multi-Crop Passport Descriptor (MCPD) data uploading program of the newly created European Vitis Database needed to recognize the old accession-number of Genres081 and to replace it by the new accession number. As a consequence and with respect to the photo file names, characterization and marker data of the previous Genres081 project, accession numbers had to be replaced by the new ones as well. It was decoded that imperatively the accession numbers designed by GrapeGen06 partners had to start with the partners institute code. Now, the MCPD data uploading program is rejecting accessions with identical accession numbers. With respect to MCPD data, the FAO / IPGRI (MCPD) format used by EURISCO, encompassing 35 descriptors, was fully adopted by GrapeGen06-partners. According to Vitis specific demands 14 further passport descriptors have been added, resulting in a total of 49 descriptors. Supplemented descriptors help to better describe the accession (criteria: berry colour, country of origin of the variety, year of crossing) and to specify facts related to trueness to type (criteria: trueness to type, variety name, confirmation of the accessions identity by ampelography, SSR-markers, bibliography, remarks to the accession name). Previous to the transfer of new data into the European Vitis Database, characterization data of Genres081 had to be adapted. The reason was that in June 2007 the 2nd edition of the OIV descriptor list for grapevine varieties and Vitis species had been accepted by the OIV General Assembly. For this 2nd edition descriptors had been completely revised. As a consequence all primary and secondary descriptor data recorded in the scope of Genres081 were converted to be compatible with the new descriptor specifications of the 2nd edition


and the characterization data recorded within GrapeGen06. With respect to all other data than MCPDs, again to achieve standardized data registering, the use of descriptor recording rules has been recommended. They had been agreed upon by the members of the ECPGR Vitis working group Palic, Serbia, June 2003 (Appendix II of the ECPGR Vitis working group meeting) and are to be found in the document describing the Multi Crop Passport Descriptors - Specific for Grapevine. Data transferred from the Genres081 to the GrapeGen06 database comprised: MCPD-Data from 18 grapevine collections Characterization data from 834 accessions Photos: 1803 pictures from shoot tips, leaves and clusters SSR-marker data from 47 accessions Data from grapegen06 partners numbers (Annex 16) : 28.085 MCPD-Data Characterization data from 2.070 accessions Photos: 3.473 pictures from shoot tips, leaves and clusters SSR-marker data from 4.086 accessions Virus data from 464 accessions. In addition MCPD data of 8 grapevine collections gathered in the scope of the Black Sea Project from 5 countries (Armenia, Azerbaijan, Georgia, Russia and Ukraine) encompassing 2096 accessions have been uploaded into the European Vitis Database (See annex 17).

Sub Task 6.2: Improvement of the database structures and utilities (sorting features, identification of synonyms from coded SSR data,…..)

Start date: January 2007 No of participating partner(s) All Partners

In the scope of the European project Genres081 (1997-2002) the first European Vitis Database has been established. Owing to new orientation of institutions competences the responsibility for the website of the European Vitis Database had changed just before GrapeGen06 started. Thus with the need to rebuild a completely new database, the opportunity to implement a future-oriented on-line uploading documentation system was given. The technical infrastructure applied for that purpose encompassed the following tools: (1) the relational database management system MySQL, (2) the internet programming language PHP, (3) JavaScript, (4) Cascading Style Sheets (CSS), (5) PEAR-Spreadsheet, (6) FPDF, (7) Ajax, (8) the operating system LINUX and (9) the web server APACHE. The implementation of a decentralized uploading system required three access levels: - public access - all partner access ((1) encompassing data not for public use and protected by a confidentiality

agreement among the GrapeGen06 partners, (2) providing separate access to WP1, WP2, WP3, WP4 and WP5, (3) offering downloading of search results)

- partner specific access (for upload of data and interactive modification of data). Protocols for descriptor recording, agreed upon by the partners and standardized file formats for all descriptor categories have been designed (See figure from Annex 18 ). They are downloadable and to be found on the “public access” level via “Descriptors/file formats”. For photo upload a standardized format for photo file names has been defined by the partners: accenumb_accename_category(_additional text).jpg. Total implemented applications are given in Annex 19 . In the following the most important innovations are


summarized: - development of modules for independent MCPD data, characterization data, SSR-marker data, virus

data and photo upload by partners via partner specific access. - development of modules enabling partners to modify their records on their own partner specific access

level: addition, updating and deletion of single records - export of MCPD, characterization, SSR-marker and virus data.

Particularities with respect to the import program for MCPD data needed to be respected. It is a universal phenomenon that even though standardized file formats are available modifications on headings, addition of columns etc. occur. Hence an import module for MCPDs was developed checking the format of the import files first. In case of none conform format, like incorrect field names, non-acceptable columns or lines, mathematical functions in excel tables, they can be modified, respectively are deleted. Furthermore in the beginning for most of the MCPDs no agreement on term standardization had been made. All data had been imported as they had been delivered by the partners. The result achieved is illustrated by the example genus, species and the species author. After having imported the MCPD data of several partners, various different spellings of the same species, including species author occurred. As a consequence several queries had to be carried out to find all accessions corresponding to the search term and respectively a single search barely displayed 100 % of the accessions concerned. Hence terminology has been defined for a series of MCPDs like berry color (green, rose, red, grey, in total 16 combinations are accepted), trueness-to-type (yes, no, uncertain, no reference, not checked) and crop use (wine grape, table grape, raisin grape, rootstock, ornamental grape, wild grape). In addition data entry had to follow fixed format rules, e.g. for criteria where the year had to be entered, the year had to be mentioned first, followed by the month and by the day, for example: 20020623. In that context institute codes, needed for the standardized entry of MCPDs like holding institution, donor, breeder, etc., can be retrieved via “Public access”/“Institute codes”. Institute codes are assigned by the World Information and Early Warning System (WIEWS) on Plant Genetic Resources, established by FAO. Finally if MCPD data are uploaded and terms do not correspond to the predetermined needs, MCPD data can be modified whilst import procedure. Implemented search modes are given in Annex 18, too. The three access levels can retrieve all data, except to SSR-marker data, reserved to GrapeGen06 partners having signed the confidentiality agreement. All levels encompass a “Quick search” option (4 criteria) and an “Advanced search” option (37 criteria) for MCPDs. Photos, characterization data, SSR-marker data and virus data can be retrieved. A selective search for grapevine varieties corresponding to specified expression stages of characteristics has been set up for characterization data. Drop down menus for notations facilitate the input of the search values. With respect to SSR-marker data coded allele lengths can be entered to search for accessions with identical profiles. The latter two search options are to be found via “Advanced search”. Within GrapeGen06 on-farm evaluation has been carried out and Vitis sylvestris populations and plants inventories have been set up. Upload modules for respective records have been implemented. At present, 218 Vitis sylvestris populations over all Europe have been surveyed and are recorded in the database. They contains on average 8.5 plants per population, a results confirming the endangered status of Vitis sylvestris in Europe. The sex ratio is near to males:females = 1:1 and only 118 plants did not display flowers, confirming the wild status of these populations (cultivated grapevines are essentially hermaphrodite). A catalogue of varieties has been implemented on public access level. It offers to users a one side description of an accession, downloadable as a pdf document. The description encompasses morphological and agronomical characteristics and photos of the shoot tip, leaves and a bunch. A handbook for users has been written, describing how to proceed to obtaining the desired information via the offered search options and explaining how data are uploaded. It is available on the public user level, as well.


Sub Task 6.3: Transfer of new data in database (SSR, primary, secondary data and pictures, information on on-farm sites, data for V. sylvestris sites and individuals including SSR)

Start date: January 2008 No of participating partner(s) P0 P2 After implementation of import applications, data upload was carried out by GrapeGen06 partners for all categories, except on-farm evaluation and Vitis sylvestris data. The latter have been imported by the European Vitis Database manager. The categories and number of recorded descriptors per category are the following:

MCPDs: 47 descriptors (e.g. accession number, accession name, holding institution, etc.) Characterization: 48 descriptors of the OIV descriptor list for grapevine varieties and species (e.g.

Young leaf: colour of upper side of blade (4th leaf) (OIV 051), Mature leaf: number of lobes (OIV 068), Berry: shape (OIV 223), Time of bud burst (OIV 301), Bunch: density (OIV 204), Bunch: single bunch weight (OIV 502)).

SSR-marker-analysis: 6 loci of Genres081 (VVS2, VVMD5, VVMD7, VVMD27, VrZAG62 and VrZAG79 plus 3 loci of GrapeGen06 (VVMD25 VVMD28, VVMD32)

Virus: Grapevine Fanleaf Virus, Arabis Mosaic Virus, Leaf Roll Virus type1, Leaf Roll Virus type 3. Photos: shoot tips, leaves and bunches. Full size photos are uploaded. A small sized copy is generated

for screen display, whereas the full size photo is used to create the pdf-document. on-farm evaluation: general description of the cultivar, vineyard, morphology, agronomic and

oenological features and results from public wine tastings Vitis sylvestris populations and plants: adapted FAO / IPGRI MCPDs.

Database contents (Annex 17) Finally the European Vitis Database registers MCDP data gathered within the two European projects GrapeGen06 and Genres081 and the Black Sea-project. Altogether inventories of 33 grapevine collections, located in 21 countries are included, maintaining 33.690 accessions. In total, Characterisation data are available for 2333 accessions of old and rare varieties, 4.086 accessions have been genotyped by SSR-marker and virus status has been checked at 464 accessions. For the number of described accessions per partner see table 2. In total the partners imported 3.731 photographs. On farm-evaluation has been carried out on 54 cultivars. With 222 locations a considerable number of Vitis sylvestris sites have been gathered, from which 631 Vitis sylvestris plants have been safeguarded and are preserved by 7 countries in 7 repositories. For detailed information see the European Vitis Database public access level and therein “on farm maintenance”, “Vitis sylvestris populations” and “Vitis sylvestris plants”. Last but not least it has to be emphasised that during implementation GrapeGen06 partners checked the European Vitis Database for functionality and made several valuable suggestions for improvements.


Work package number WP 6 Start date.: January 07..... Month 1 Work package title: Database improvement and enrichment

Conclusions

Conclusion Data upload carried out by collection holders themselves disburdens the database manager from a time-consuming task. During the Genres081 project numerous file formats, photo names and data of partners had to be readapted by the database manager previous to import. Data set modifications requested by partners had to be carried out by the database manager as well. In contrast to that experience, the uploading and interactive modification system represents a real progress. The fact that curators don’t need an intermediate to upload their data increases the probability of further data updating and hence database enrichment. Upload is possible any time and there is no risk to disturb the database manager. Export of all kind of generated data and photos assists in comparison and thus helps identification of accessions. In particular exported MCPD data provide standardized data sets. Therefore if an updating becomes necessary, it is recommend exporting MCDP data, to add or modify records and to use this file for import, thus avoiding time-consuming adaptation to the requested standards. With respect to the easiness of data import and data administration the acceptance of further collection inventories is realistic. To continue gathering grapevine diversity further partners in particular from countries not present in the European Vitis Database is envisaged. With the implementation of this future oriented tool, a basis has been laid to establish a network of grapevine collections with true to type accessions and organizing duplicate conservation for endangered genotypes.




Work package title: Elaboration of a long term conservation strategy

No of lead partner P00 No of participating

partner(s) All partners

Main objectives

Global objective of WP7 : Elaboration of a long term conservation plan for EU genetic resources

Objective 1 : Development of alternative methodologies for conservation (cryo, on farm, ex-situ initiatives for V. sylvestris …) Objective 2 : Introduction of endangered cultivars and V. sylvestris accession in the collections Objective 3 : Safety duplication of variety and clonal material in collections Objective 4: Dissemination of data

Sub Task 7.1 : Development of cryo-conservation techniques and collections

Start date.: January 07..... No of participating partner(s) P00 The cryopreservation section of the GrapeGen06 project was possibly the most prospective one as little data was available on many of these aspects at the onset of the project. The project was carried out under the responsibility of Dr J.P. Péros up to April 2008, then of Dr Ph. Chatelet onwards with the collaboration of colleagues at IRD (principally Dr S. Dussert). Main results Seed cryopreservation An original protocol has been designed (collaboration S. Dussert, IRD) and implemented on wild species accessions from the Vassal grapevine repository. A pip cryobank has therefore been initiated with seed lots (see tables 1 and 2 ). From 2009 pip cryopreservation campaign (sixteen accessions from the Vassal collection), test samples displayed post-thawing germination percentages from 8 to 98%, confirming protocol effectiveness and the stimulating effect on germination sometimes observed in LN treated seed lots (mean germination rates for control seed lots 47% opposed to 61% post thawing), likely related to integument micro-rupture leading to seed increased access to water and oxygen. Through GrapeGen06 contacts, seeds from V. vinifera sylvestris wild populations collected in Germany (Dr Erica Maul) and Italy (Dr Anna Schneider) were sent to Montpellier and cryopreserved using the protocol developed. Pollen cryopreservation An improved medium for pollen in vitro germination has been proposed. A protocol for pollen cryopreservation has been designed and a pollen cryobank has been initiated (see table). If fecundating ability of cryopreserved pollen could not be tested yet, post-thaw in vitro germination has not been found significantly reduced when working with purified pollen samples. Bud cryopreservation: No published data were available on grapevine dormant buds cryopreservation, so a complete routine had to be set up and implemented in our conditions. A fully working protocol to ensure dehydration follow-up has been successfully adapted and implemented on grapevine providing a precise estimate of bud actual moisture content. A disinfection method has been designed ensuring over 90% recovery of axenic explants from field-grown vines.

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Valorization to date Please note: In order to ensure optimal valorization, all detailed protocols will be made publicly available as soon as the corresponding scientific papers have been accepted for publication in suitable journals, although interested GrapeGen06 partners will be given full details and assistance on simple request. Seed (pip) cryopreservation: an oral communication at international level has been delivered in 2009 (First ISHS Symposium on Cryopreservation in Horticultural Crops, Leuven, April 2009). A corresponding scientific paper is to be published in 2011 in collaboration with colleagues at IRD. The corresponding validated protocol has been written in full, and submitted with annual report 2008. Pollen cryopreservation: a working protocol has been written in full and submitted with annual report 2009. Bud cryopreservation: A tentative protocol awaiting full validation has been written with a complete description of the methodology currently used for node dehydration, cooling, thawing and meristem culture. Protocol is to be submitted together with Technical report 2010, although dissemination will not be carried out until additional results allow to improve its efficiency. Training: A 10-day training session on dormant bud cryopreservation methods applied to grapevine has been provided to one project member (P. Jadrna, Czech Republic) in 2009; After an initial 6-month training period in 2010 in Montpellier (encapsulation/dehydration and droplet vitrification cryopreservation techniques) developed on in vitro grapevines (collaboration with Partner 1 - IRD Montpellier), Miss Zvjezdana Marković, MSc, teaching assistant at the Faculty of Agriculture, Zagreb (partner 20), has enrolled in a doctorate thesis (collaboration U. of Zagreb/ Montpellier SupAgro) on cryopreservation and cryotherapy techniques on in vitro grown grapevines (joint supervision Prof. J. Kontić/Dr F. Engelmann) Conclusion In conclusion, we demonstrated that cryo-conservation of pollen and seeds is a viable option to preserve allelic diversity in grapevine. This appear to be a very good option to preserve wild grapevine diversity, since the genotype (cultivar) in this case is not important, while allelic diversity is. Conserving the wild genetic diversity in liquid nitrogen is however a static option, i.e. individuals are not allowed to intercross, recombine, and thus adapt or evolve under mutating environmental conditions. Cryo-conservation is thus considered an ultimate strategy for safeguarding genetic diversity when there is a large risk of extinction (local or genus-wide). Since in our evaluation, the wild grapevine populations are effectively at risk of extinction (see the very low average number of sexual mature plants in wild population inventories, WP4), cryo-conservation is a pertinent option for wild grapevines. For the cultivated compartment, where the genotype of plants has a significance (if we want to preserve old autochthonous cultivars, for example), important progresses were obtained for the first time, since this project was the first in the scientific community to be able to propagate one cultivar from cryo-preserved bud meristems. Nevertheless, we acknowledge that the technique is still quite sensitive to starting conditions (year of harvest / conditioning of material / specific cultivar) and further work is needed before this option could be made available on a large scale to satisfy the general objective of grapevine cultivars preservation. This project being finished on December 2010, further work will be carried out externally to this project. Finally, this finding stress the importance to keep and maintain fully developed cultivar collections with living plants in the field, such as those described in WP2 and develop safeguarding strategies


based on living plants.

Sub Task 7.2: Introduction of material in field collection (including safety duplication) or on-farm

Start date.: January 08 No of participating partner(s) All partners

Since the beginning of the project, several accessions were exchanged between the collections for safety duplication . During the four years of the project, a total of 164, 42, 164, 149 and 510 accessions were exchanged in 2006, 2007, 2008, 2009 and 2010/2011 respectively (Annex 21). Similarly, a total of 486 wild accessions have been introduced during the 4 years by the Grapegen06 partners (Annex 22).

The exchanges involved, as donor or beneficiary, 14 project partners and one external partner [University of Milan]). Partner 0, holding the biggest collection from any of the partners was the one to send most of the material.

The principles for choosing the cultivar to exchange were : they should be of interest for the receiving partner (possibly old national cultivars that had been lost in the receiving Country), they should be old autochthonous varieties, they should be rare ones, represented only once or twice in European collections and thus worth duplicating.

Conclusion:

The exchange of plant material was thus quite important during the project, even if relatively small in regds to the 28 thousands accessions hold in the collections. It nevertheless represents a good way to ensure safer conservation of rare and important cultivars.

Sub Task 7.3: Development of in-situ conservation procedures

Start date.: January 08or 09 No of participating partner(s) For wild grapevine, in situ conservation procedure needs the identification of natural populations to be conserved, information about their status as well as if they are at risk of destruction because of other development projects at the local level (such as construction of roads, urbanisation, industry etc.). This work of data collection was done in WP4 and the data available on the EVDB. One decision made in this sub-task was to prepare a letter for raising the awareness of local territorial authorities, and send it out to those with geographical competence on the described populations. The letter is on Annex 10. It was sent out by the concerned partners together with a geographical map of the population. Other initiatives were taken such as contacting national or regional parks for establishing conservation areas within their land (examples in Piedmont, Italy, or the Botanic Conservatory of Porquerolle, France), but the time was too short for these contacts to be finalised with an agreement. In some cases the wild populations were found not belonging to a public entity and thus it is not possible to expect a constructive contact with a private owner, which in addition appears of very difficult identification. The best we can aim with this project is to raise awareness of local environmental authorities to wild grapevine conservation, and possibly to raise their interest for asking us cuttings or seeds of identified wild genetic resources for reintroduction on their land. We found out that in this project we can not force local authorities


(mainly regional parks), to introduce new grapevines, nevertheless we hope that they have got the message and something can be developed in the next years after the end of the project. Several identified wild populations entered into other national projects, such as a project funded by the French National Agency for Research or the Ministry of Environment of Georgia. Overall, we believe that the fact that these populations were identified by an European project will add credibility to future initiatives for in situ conservation. For cultivated grapevines, several partners promoted local initiatives for spreading and introduction of autochthonous cultivars on farm, in particular under WP3. Many of the autochthonous cultivars studied were completely lost on farm and were re-introduced with materials remained available in collection only. All our collections have an important activity of spreading lost or rare cultivars to interested growers. This activity is thus considered an in situ conservation activity and can be an example of successful reintroduction and enrichment of local cultivar diversity. For exemple, P11 reintroduced several neglected autochthonous cultivars completely lost by the production sector of Hungary, and available today only in germplasm collection, to a winery at Somló hill (Kreinbacher Birtok Kft.). This winery was interested in the reconstuction of the famous medieval and new age wines of the region. Based on the historical documentation, the original assortment and ratio of cultivars were reconstructed. Since nowdays, only the Kéknyelű, Sylvaner green and the Furmint are in production, the other cultivars cited in the history of the region: Mézes, Lisztes fehér, Góhér, Bakator, Sárfehér, were reintroduced to the Kreinbacher Birtok winery from the gene bank of the Institute of Viticulture and Enology-Pécs, where they were preserved in 10-10 stock parcells. All partners participating to WP3 took initiatives in this direction. In total, our project raised the interest and actual in-situ conservation of 59 old forgotten autochthonous varieties, which are presently well-described both in term of morphology and agronomic characteristics as well as for their wine characteristics. These 59 old autochthonous varieties are now conserved in 115 agricultural fields on-farm, each variety being on average conserved in 2 different vineyards (annex 4). This duplication ensures conservation and an optimal risk management regarding possible mortality, on the medium/long-term. Since in each vineyard the number of plants is always above the hundred, we believe our project succeeded in demonstrating that the revitalisation of old rare cultivars is feasible and interest the viticulturists. In addition, the publicity given to these on-farm old autochtonous varieties through seminars, wine tasting and agricultural fairs (list of publications) raised the interest of other viticulturists willing to spread these interesting novelties in other viticulture situations, with the objective to feed the market demand for regional quality products. With the spreading of these varieties, we believe our project has shown in practice the feasibility of reintroducing old rare regional varieties on in-situ situations, promoting conservation and spreading of valuable old rare neglected cultivars in Europe.

Sub Task 7.4.: Development of a management plan (2009-2019) for Vitis resources

Start date...... No of participating partner(s) All During the project, the work on the management plan was concentrated at the end of the action, since we needed the data produced by the other projects. The management plan is presented in Annex 23.

Sub Task 7.5.: Coordination of the project

Start date......


No of participating partner(s) All partners P..

See section The coordination of the project was done by the scientific coordinator (P This), and the administrative coordinator (R Bacilieri), with the help of the executive committee (composed of: C Taillaferie, contract office P00 ; T Lacombe, curator of the field collection of Vassal P00 ; V Laucou, molecular biologist P00 ; JM Boursiquot, ampelography and viticulture P00 and P14 ; E Maul database curator P01), and of the governing board (7 WP managers). The coordination along the year concerned essentially data centralization and organization, from the partners to the coordinator and to the database. The main problem of inconsistency or late arrival of MCPD data was addressed and solved by a note to all partners from the database curator. Few problems of SSR coding were also addressed bilaterally by the coordinators and the partners. In general, the coordinator observed a large need, among partners, of explanation and training concerning protocols, procedures, data coding and uploading. Each partner needs an individualized follow-up along the process of data preparation and uploading. This was provided all along the year by the administrative coordinator on a partner-by-partner basis (208 E-mail conversations were held among the administrator and single partners in 2010 only). In several significant cases the training was provided by the WP leaders, such as for WP5 (questionable work) and WP6 (database functioning). A large effort was also devoted to find measures to avoid uploading of low quality data (containing mistakes, false alleles, etc.). Present data uploading (2007-2010) has undergone large reviewing and double checking by P00, P08 and P20 (WP leaders) in 2010, so that that part is now considered error-proof. For the future (2011 and beyond) and especially as far as new partners and contributors (not part of the present project) are concerned, a protocol has been published on the database. Continuous training will be provided by P00 and P01 and new partners will only be allowed to enter new data if they accomplish all the expected step-by-step procedures. General management decisions were taken, by the coordinators, first consulting all the partners, and then for the main points, by organizing a vote and obtaining general approval. Decisions and instructions were put on the project website. The annual project meeting was held at the P01 Institute, JKI Siebeldingen (Germany), on June 1st and 2nd, 2010. The meeting was attended by 25 persons from 17 partners. The other partners were consulted on the main decisions to be taken by mail. A final meeting was organized in 2011 by P00 in Montpellier (France), involving the WP leaders only, with the objective to finalise the analysis and the final technical documents.

Work package number

WP 7 Start date.: January 07..... Month 1

Work package title: Elaboration of a long term conservation strategy

Conclusions

The protocols for seed and pollen cryoconservation are ready, seed and pollen have already been conserved. Very promising results were obtained for dormant buds but unfortunately this still cannot be proposed as an alternative for the management plan. The introduction of new material in the collections and the exchange of material as safety have been quite abundant in 2010. The “Official European Catalogue” was finished in 2010 and will be published in 2011 and will be an important part of the management plan. SSR and morphological data have also proven their usefulness for the comparison of the database.

Finally a management plan was proposed for the 10 next years of grape genetic resources management and characterization.

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4.SUMMARYOFTECHNICALPROBLEM(S)ENCOUNTERED(IFANY)

Technical problem(s) and solution(s)

Preceding Years: Only the main problems that modified the course of the project are listed here. Other problems, were resolved during the course of the project and had little effect on the running of the project. 2007 WP4: For this WP, problems were identified concerning several definitions:

how to define a site, a station and how to link these physical notions to the definition of populations which is important for the survival of the plant material.

The subjectivity of the preservation status Discussions or a specific workshop have been conducted in order to reach common definitions. .WP6: The development of the database took longer that expected, and there has been a slight delay in availabilities of the different functions of the databases. The final product have been made available during 2009. 2008 The biggest problem the project had to face was the defection of P17: because of financial problem the Slovakian government decided to close the institute. Plant material was send to Montpellier and P00 will do the SSR analyses that were planned by P17. Partner P17 (Slovakia) has performed most of its tasks and a fair amount of work concerning WP2 and WP4 (all the wild populations and individuals have been identified and recorded ; more than 60 living samples (cuttings) were sent to P00 for further characterization and duplication). The analysis of DNA from these wild individuals has been performed and replicates of them have been planted in the Vassal collection (France) by P00. 2009 The biggest problem the project faced was the absence of work by some of the non-funded partners. Despite the fact that the partners agreed to participate to the project, the funding from their agencies were not increased and they could not participate in the Analysis. Only the Maroccan and the Georgian partners were able to perform initial planed works. Additional work has been made by the other partners to replace their work. Regarding the work from P04, this partner encountered severe technical problems with the automatic sequencer. The group decided that the DNA should be send to P15, which performed the analysis for P4 and send the data by September 2009 to both WP1 coordinator and P04. Part of P04's budget have thus been transferred to P15 for this work. On November 2009, P0 delivered to P4 80 full coded genotypes while, due to low DNA quality, 24 samples are still missing. They were tried again in 2010 in collaboration between P04 and P15. Because MCPD data for P4 were not uploaded, this data could not be uploaded in the database. CONCLUSION: No technical problems remained unsolved at the end of the project. The amont of data produced was according to the plan.


5.SUMMARYOFTHEMANAGEMENTOFTHEACTION

Meetings, workshops and seminars organised by the coordinator and the partners

Preceding Years: 2007 The initial workshop was held in Versailles, France from 21st to 23th of March, 2007, in order to start the action. The Minutes of the workshop were transmitted to the DG-Agri end of April 2007. All the non-eligible partners as well as INRA and Bioversity International attended a meeting entitled “Conservation and sustainable use of grapevine genetic resources in the Caucasus and Northern Black Sea region” organized jointly with the Kuban State Agrarian University from 15 to 17 October 2007 in Krasnodar (Russia). During 2007 only two meetings were organized because the need of other ones did not arise. The coordinator may have to be more cautious about the arising need next year and should encourage WP leaders to organize specific work shop or meetings using any mean 2008 The second meeting was organized in Godollo by Erzebet Kiss (P13) . The minutes of the meeting are presented in annex 10 This meeting have been important in the management of the project since we have discussed about most of all aspects of the project. The next meeting will be held in Italy during sumer time Preceise date to define). Several scientists have been invited to this meeting either to introduce them to GrapeGen06 project or in order to start some collaboration (ex. Claudio d’Onofrio involved in the development of a database). 2009 Stella Grando (P08) organized the third meeting in San Michele. This meeting have been important in the management of the project since we have discussed about most of all aspects of the project. Several scientists have been invited to this meeting (see annex 11) either to introduce them to GrapeGen06 project or in order to start some collaboration (ex. Claudio d’Onofrio , S. Imazio, A. Jung who agreed to send data to the project. Final Year The 2010 meeting was organized in Germany at the Geilweilherhof institute by Erika Maul. The minutes of the meeting were posted on the web site at the following address : This last meeting have been important in the management of the project since we have discussed about the finishing of the work and how to proceed with the data analysis and the preparation of the final scientific journals.


CONCLUSION Nevertheless, the finishing of the project has been difficult. The partners were quite slow to send the data. A final meeting meant for the writing of the reports and of the papers was organized in Montpellier on June 1st 2011 for the coordinators. All coordinators attended this meeting except Ivan Pejic and Jose Eiras-Dias who participated through visio-conference.


Changes in the action's management structure (If any)

A number of changes in the management structure (names and locations of the Institutes, or in the persons managing the action) were brought to the attention of DG-Agri with an electronic mail and attached files on 23/11/2007 (R. Bacilieri to O. Diana copy DG-Agri). We summarise the most important ones : ALREADY TRANSMITTED MODIFICATIONS (23/11/2007) 1), In contrast to what is written on page 66 of the annexes to the contract (annex IIb), the exact partner numbers should be :

Partner n. Acronym Country Postal code, town 0 INRA France F-34000 Montpellier 1 BAZ – IRZ Germany D-76833 Siebeldingen 2 HBLAuBA Austria A-3400 Klosterneuburg 3 IMIDRA Spain E-28800 Madrid 4 NAGREF Greece GR-14123 Lykovrissi 5 EVN - INIAP Portugal P-2560 Dois Portos 6 CRA – VIT Italy I-31058 Susegana - Conegliano 7 CNR-IVV Italy I-10095 Grugliasco 8 IASMA Italy I-38010 San Michele all' Adige 9 DISAA - UNIUD Italy I-33100 Udine

10 DiSteMeV Italy I-37029 S. Floriano 11 AGROSCOPE-RAC Switzerland CH -1009 Pully 12 FVM SZBKI Hungary H-7634 Pécs 13 SIU DGPB Hungary H-2103 – Godollo 14 RICP Czech Republic CZE-26718 Karlstein 15 IFV France F-34000 Montpellier 16 ARI Cyprus CY-1516 Nicosia 17 VŠSVVM Slovakia 900 01 Modra 18 INVV Moldavia MD-2019 Kishinev 19 GRI Azerbaijan AZ-1106 Baku 20 FAZ Croatia HR-10000 Zagreb 21 IBB Georgia GE-Tbilisi, 0159 22 Institute of Botany Georgia GE-0105Tbilisi 23 IHVO Georgia GE-Tbilisi, 0159 24 UFR BV Morocco 93000 Tetouan

Using these numbers allows us not to create additional confusion by changing all the specific informations spread over all the contract, concerning the partners references in the WP descriptions [p. 56 to 63] and in the general tables (involvement of institutes by tasks [p. 44], personnel by WP [p. 64]), etc. 2) On page 66 of the annexes of the contract there was an error in the name of the Czech partner, which is not “Research Station Viticulture” but “Research Institution of Crop Production” (RICP) 3) INRA : Mr. Philippe Vissac replaced Mr. Bernard Itier as President of the INRA Centre of Montpellier and by consequent as representant of INRA for the present contract (page 1 main contract). 4) ENTAV became ENTAV-ITV according to a decision of the French governement 5) University of Verona replaced University of Florence 6) The German partner BAZ has informed of a change in his Bank coordinates 7) There was a change in the management persons of the CRA-ISV in Italy, due to end-of-carrier retirements. The new scientific manager is Ms . Manna Crespan in the place of Angelo Costacurta, and the new financially responible person is Dr. Romualdo Coviello in the place of Antonio Calo' 8) We corrected some approximation errors in the tables A.3.5, B.1.6, B.2.4, and B.2.2 (when we approximated 0,5 month to 1)


NEW MODIFICATIONS proposed after 23/11/2007 1) ENTAV change its name in Institut Français de la Vigne (IFV). 2) There was a new change in the management persons of the CRA-ISV in Italy, starting from 21/12/2007 the person financially responsible is Dr. Giovanni Lo Piparo (in the place of Romualdo Coviello). Furthermore, the Institute changed its name, the present being : Consiglio per la Ricerca e la sperimentazione in Agricoltura - Centro di Ricerca per la Viticoltura ; acronym : CRA-VIT 3) Several Institutes informed us of transitional changes owing to their government re-structuring the national research system. This is the case of

a) The FVM Szöleszeti es Boraszati Kutatointezete (Hungary), the status of indipendent Institute of which was recently taken away with the objective of (probably) integrating it with the University of Pécs. However on 25/01/2008 P. Kozma wrote to me “The position of the Institute is still not certain. We have budget only up to February”. One direct consequence is that our plan to organise the next 2008 workshop in Pécs cannot be practically pursued in these conditions, and we have to find a new host institution.

b) A similar situation occurs at the Research and Breeding Station for Viticulture of Modra (Slovakia). This once large Institute went under several restructurations and it is at present a government-funded non-profit organisation. However the financial resources of 2008 are not yet delivered and salaries of 2008 are not being paid yet (information coming from D. Pospisilova on 14/02/2008). The same delays, of course, affects the running budget for the GrapeGen06 action. We are in close contact with them and will keep the DG-Agri promptly informed.

c) Similar difficulties touch the Georgian grapevine collection, since the surface where the grapevine resources are grown near Tbilisi (by D. Maghradze, IHVO) was sold to a supermarket, and the government did not deliver yet any fund for moving the resources. However the salaries are not in question in this case. We have already done a very satisfactory work with D. Maghradze.

d) Other institutional changes also affected the IASMA and CRA-VIT in Italy, as well as the University of Verona which replaced of the Un. of Florence. This brought some difficulty to carry out the work at the expected speed, mainly because of difficulties to spend the funds given the undergoing modifications in the financial structure of the institutes. However for these three latter Italian cases we are sure that we can catch up the delays in 2008.

We suppose that these situations and especially those of the Eastern European and non-European Countries have to be watched carefully, with an objective of encouraging and supporting the partners for their extraordinary efforts (in particular the three institutes from Hungary, Slovakia and Georgia provided or are providing to the project a large number of accessions of wild grapevines or old autochtonous varieties, a very important contribution) and not sanctioning them further.



Distribution of the labor in the project

The project was structured in 7 WPs, of equal importance for the project, but not necessarily of equal importance in term of man-power. So WP2 and WP7 represent more than half of the project.

Wp2 is based on the analysis of plant material in the field. It is rather more time consuming than other WPs because the analysis is made all along the growing seasons and evolved lots of traits to be analysed on the plants. The main justification of this WP is thus man-power.

For WP7, most of the partners did work on this aspect since all partners were involved

(which is not the case for all the WPs). Much work was also performed on this aspect by P0, most especially for the administrative aspects that were very heavy due to the number of partners and the demand of the European commission. In this WP, we also included the work on the cryoconservation. This part of the project did not go as planned and much more work was performed that originaly planed.

Similarly, the work was not necessarily identical between the partners:

P0 as the coordinator of the project and because in charge of the development of the cryoconservation methods (the most innovative aspects of the project) was the partner who declared the most man-power.

P11 declared the second most man-power because it was involved in both WP2 but also perfomed much work on WP3 (also quite intensive in term of man-power).

P1 was in charge and perform all the work for WP6, which even if not important in comparison to the whole project, nevertheless consisted in a quite important aspect of the project.

P13, was a new partner of the project and therefore needed to catch up with the project. This partner performed an important amount of work for the prospection of wild individual in it’s country.


6.SUMMARYOFTHEDISSEMINATIONOFALLTHERESULTS

During the four years of the project, the dissemination of the results have been performed under 4 types of communications : as documents published on the database, as publications in scientific journals, in journals and magazines for growers and general public, and as disseminations during scientific symposiums and dissemination for general public and stakeholders including tasting of wine. The final section list several documents still in preparation. Even if the project is officially finished, as for every project, data is still being analysed, paper and or presentation are still in progress.

Documents published on the database

A handbook for users has been written, describing how to proceed to obtain the desired information via the multiple search options. It is available on the public user level. An appropriate disclaimer for intellectual property of data, citation when using data, and no responsibility assumption for their use was also made available.

A manual for standardization has been submitted by partner 3 with 90 pictures of different examples included in the descriptors used. The manual will be also published in the European Vitis Database.

Publications in scientific papers

The publications are listed by year and by alphabetical order 2007

Pospíšilová, D.: Úloha lesného viniča vo vývojovom procese súčasných odrôd (Role of woody grapevine in the evolution process of the contemporary cultivars). Vinič a víno, 2007, No. 2, 34-35 pp.

Pospíšilová, D., Šimora, R., Valachovič, M.: Prieskumná a zberová expedícia lesného viniča Vitis vinifera ssp. sylvestris Gmel. (Investigation and collection of Vitis vinifera ssp. sylvestris Gmel.). Spravodajca Genofond (Newsletter Genofond), 2007, No. 11, 32-33 pp.

2008

Cunha, J., J.P. Cunha, L.C. Carneiro, P. Fevereiro, J.E. Eiras-Dias, 2007. Carpometria da graínha na identificação de ancestrais selvagens de Vitis vinifera L. Ciência Téc. Vitiv. 22 (2), 29-34.

Cunha, J., Margarida Baleiras-Couto, J.P. Cunha, Jorgete Banza, Adelaide Soveral, L.C. Carneiro, J.E. Eiras-Dias, 2007. Characterization of Portuguese populations of Vitis vinifera L. ssp. sylvestris (Gmelin) Hegi. Genet Resour Crop Evol 54, 981-988.

Institute of Horticulture, Viticulture and Oenology (2008) Grapevine and Fruits Germplasm of Georgia” (The book in Georgian of collected research articles was composed by our editorial leading at the Institute of Horticulture, Viticulture and Oenology).

Maghradze D., Chkhartishvili N., Vashakidze L., Kvaliashvilli V. (2008) Georgian germplasm of grapevine and fruits: current situation, investigation and utilization (This book includes original articles and translated works on Georgian language, among which are also information about the GrapeGen06 project).


Pospíšilová D., Sekera, D., Šimora, R..Old vine cvs. cultivated in Slovakia. 1. part : Varieties. Vinařský Obzor (Czek Republik), sent for Nb.11/2008

Pospíšilová, D., Sekera, D. Šimora, R.: Projekt EÚ GrapeGen06 a slovenská účasť v ňom (The project EU GrapeGen06 and the Slovak participation on it. Vinič a víno 8, 2008, 1, p.10

Pospíšilová, D., Šimora, R., Sekera, D.: Old vine cvs. of Slovakia. 2. part: Scientific work. Vinařský obzor (Czek Rep.) Sent for Nb. 12/2008

Vakhtangadze T., Maghradze D., Chipashvili R., Abashidze E., 2008. Role of Georgian native varieties of grapevine in breeding. Viticulture and Winemaking “Magarach”. Yalta. 3: 11-13. (In Russian)

2009

Cunha, J., 2009. Diversidade biológica de Vitis vinifera L. em Portugal: contribuição genética da subsp. sylvestris (Gmelin) Hegi para a origem das castas Portuguesas, subsp. vinifera. 183pp. PhD thesis, UN Lisboa, Oeiras.

Cunha, J., Margarida Teixeira Santos, L.C. Carneiro, P. Fevereiro, J.E. Eiras-Dias, 2009. Portuguese traditional grapevine cultivars and wild vines (Vitis vinifera L.) share morphological and genetic traits. Genet Resour Crop Evol 56, 975-989.

Maghradze D, Failla O, Turok J, Amanov M, Avidzba A, Chkhartishvili N, Costantini L, Cornea V, Hausman JF, Gasparian S, Gogishvili K, Gorislavets S, Maul E, Melyan G, Pollulyakh A, Risovanaya V, Savin G, Scienza A, Smurigin A, Troshin L, Tsertsvadze N, Volynkin V., 2009. Conservation and sustainable use of grapevine genetic resources in the Caucasus and Northern black Sea area. Acta Hort (ISHS) 2009, 827, 155-158

Meneghetti S., Costacurta A., Crespan M., Maul E., Hack R., Regner F., 2009 Deepening inside the homonyms of Wildbacher by means of SSR markers Vitis 48 (3): 123-129.

Rodriguez-Torres I., Ibáñez J., De Andrés M.T., Rubio, Borrego J., Cabello F., Zerolo J., Muñoz-Organero G., 2009. Synonymous and homonymous of “Malvasia” cultivars (Vitis vinifera L.) existing in Spain. Spanish Journal of Agricultural Research. Vol. 7, Nº 3.

2010

Cunha J, Teixeira Santos M, Brazao J, Carneiro LC, Veloso M, Fevereiro P, Eiras Dias J, 2010. Genetic Diversity in Portuguese native Vitis vinifera ssp vinifera and ssp sylvestris In : Holubec V (Ed.) “Monitoring, Collection and Conservation of Landraces and Wild Plant Genetic Resources in-situ and on-farm”. Chzech Journal of Genetics and Plant Breeding. Vol 46, Special Issue, Prague 2010.

Cunha J, Teixeira Santos M, Carneiro LC, Fevereiro P, Eiras Dias J, 2010. Vitis vinifera ssp sylvestris (Gmeli) Hegi populations in southern Portugal : Assessing the genetic diversity for its future management and conservation. In : Holubec V (Ed.) “Monitoring, Collection and Conservation of Landraces and Wild Plant Genetic Resources in-situ and on-farm”. Chzech Journal of Genetics and Plant Breeding. Vol 46, Special Issue, Prague 2010.

Ekhvaia J, Akhalkatsi M, 2010. Morphological variation and relationships of Georgian populations of Vitis vinifera L. subsp. sylvestris (C.C. Gmel.) Hegi. Flora 205: 608-617.


Gardiman M. (2010). Recupero, conservazione e valorizzazione delle risorse genetiche viticole. Riv. Vitic. Enol., n. 1-2-3-4/2010: 7-12.

Gardiman M., Borgo M., Giust M. (2010). Vite. In: Piotto B., Giacanelli V., Ercole S. (A cura di), 2010. La conservazione ex situ della biodiversità delle specie vegetali spontanee e coltivate in Italia. Stato dell’arte, criticità e azioni da compiere. Manuali e linee guida ISPRA 54/2010. 63-65.

Gardiman M., Giust M., Carraro R., Barile M. (2010). Il ruolo delle collezioni CRA-VIT nella conservazione, caratterizzazione e valorizzazione della biodiversità viticola. Riv. Vitic. Enol., n. 1-2-3-4/2010:13-18.

Giannetto S., Caruana R., La Notte P., Costacurta A., Crespan M. (2010). A survey of Maltese grapevine germplasm using SSR markers. AJEV, 61 (3): 419-424

Giannetto S., Caruana R., La Notte P., Costacurta A., Crespan M., 2010. A survey of Maltese grapevine germplasm using SSR markers. AJEV, 61 (3): 419-424.

Goryslavets S. , Risovanna V., Bacilieri R, Hausman J.F., M. Heuertz.. 2010. Genetic relationships of four recently bred Ukrainian wine grape varieties using microsatellite markers. Cytology and Genetics, 44:95-102.

Jandurova O, Casal R, 2010. Successful reintroduction of ancient grape varieties in the Czech Republic. In : Holubec V (Ed.) “Monitoring, Collection and Conservation of Landraces and Wild Plant Genetic Resources in-situ and on-farm”. Chzech Journal of Genetics and Plant Breeding. Vol 46, Special Issue, Prague 2010.

Katula-Debreceni D, Szöke A, Veres A, Heszky L, Kiss E, 2010. Management and conservation of grapevine genetic resources and the GrapeGen06 project. Hungarian Agricultural Research, 2010, p. 9-12.

Pospisilova D, Simora R, Sekera D, 2010. Research work on Vitis vinifera ssp sylvestris in Slovakia. In : Holubec V (Ed.) “Monitoring, Collection and Conservation of Landraces and Wild Plant Genetic Resources in-situ and on-farm”. Chzech Journal of Genetics and Plant Breeding. Vol 46, Special Issue, Prague 2010.

Salayeva S, Akhundova E, Mammadov A, 2010. Evaluation of DNA Polymorphism among Cultivated and Wild Grapevine Accessions from Azerbaijan. Czech Journal of Genetics and Plant Breeding. 46, 2:75-84.

Schneider A., Torello Marinoni D., De Andrés M.T., Raimondi S., Cabello F., Ruffa P., Garcia-Muñoz S., Muñoz Organero G., 2010- Prié blanc and Legiruela: a unique grape cultivar grown in distant European regions. J. Int. Sci. Vigne Vin, 44, 1, 1-7.

Torello Marinoni D., Raimondi S., Ruffa P., Lacombe T. Schneider A., 2009. Identification of grape cultivars from Liguria (north-western Italy). Vitis, 48 (4), 175-183.

Vrana P, Jandurova O, 2010. Must characteristics of selected grapevine varieties used for local wine production. In : Holubec V (Ed.) “Monitoring, Collection and Conservation of Landraces and Wild Plant Genetic Resources in-situ and on-farm”. Chzech Journal of Genetics and Plant Breeding. Vol 46, Special Issue, Prague 2010.


2011

Lacombe T., Audeguin L., Boselli M., Bucchetti B., Cabello F., Crespan M., D’Onofrio C., Eiras Dias J., Ercisli S., Gardiman M., Grando S., Imazio S., Jandurova O., Jung A., Kiss E., Kozma P., Maul E., Maghradze D., Martinez C., Muñoz G., Pátková JK., Pejic I., Peterlunger E., Pitsoli D., Preiner D., Raimondi S., Regner F., Savin G., Savvides S., Schneider A.,Spring JL.,Szoke A., Veres A., Boursiquot JM., Bacilieri R., This P. 2011. European Grapevine Catalogue: Towards a Comprehensive List. Vitis Journal 50 :65-68 (http://www1.montpellier.inra.fr/grapegen06/page_results/EU-catalogue.php).

Maul E, Sudharma KN, Kecke S, Marx G, Müller C, Audeguin L,Boselli M, Boursiquot JM, Bucchetti B, Cabello F, Carraro R, Crespan M, Eiras Dias J, Ekhvaia J, Gaforio L, Gardiman M, Grando S, Gyropoulos D, Jandurova O, Kiss E, Kontic J, Kozma P, Lacombe T, Laucou V, Legrand D, Maghradze D, Marinoni D, Maletic E, Moreira F, Muñoz G, Nakhutsrishvili G, Pejic I, Peterlunger E, Pitsoli D, Pospisilova D, Preiner D, Raimondi S, Regner F, Savin G, Savvides S, Schneider A, Sereno C, Simon S, Staraz M, Zulini L, Bacilieri R, This T, 2010. The European Vitis Database (www.eu-vitis.de) – a technical innovation through an on-line uploading and interactive modification system. Submitted to Vitis.

Righetti S., Boselli M., 2011. Caratterizzazione genetica e attitudini produttive della varietà Righetti (già Arcé) reperita nella Valpolicella (provincia di Verona). VQ, settembre. In press.

Disseminations to scientist

2007

Pospíšilová, D., Šimora, R., Valachovič, M.: Výskyt Vitis vinifera ssp. sylvestris Gmel. na Slovensku a možnosti jeho uplatnenia v šľachtení viniča (Occurrence of Vitis vinifera ssp. sylvestris Gmel. in Slovakia and possibility of its apply in the grape improvement). Zborník zo 14. medzinárodnej vedeckej konferencie : Nové poznatky z genetiky a šľachtenia poľnohospodárskych rastlín (Proceedings of the 14th International Scientific Conference : New Knowledge’s of Agricultural Plant-Genetics and -Breeding), Piešťany, november, 2007, 70-73 pp. 2008

Pospíšilova, D., Šimora, R., Sekera, D.: Research work on Vitis vinifera ssp. sylvestris Gmel. in Slovakia. 2nd Int. Monitoring Collection and Conservation of Landraces and Wild Plant Genetic Resources in situ, on farm. CRI-Praha-Ruzyně, 3. 12. 2008. Papers will be printed in Proceedings

2010

Maghradze D., Failla O., Bacilieri R., Imazio S., Vashakidze L., Chipashvili R., Mdinaradze I., Chkhartishvili N., This P., A. Scienza, 2010. Il germoplasma georgiano di vite: utilizzo, conservazione e studio. III Convegno Nazionale di Viticoltura. Fondazione Edmund Mach, San Michele all’Adige (Trento) 05-09 luglio 2010.

R. Bacilieri, G. De Lorenzis, O. Failla, S. Imazio, D. Magradze, A. Scienza, P. This. 2010. Molecular survey of Georgian traditional grapevine genetic resources. 10th International Conference on Grapevine Breeding and Genetics. Geneva, 1-5 August 2010.


Schaal B., Beck J., Hsu S.-C. , Beridze T., Gamkrelidze M., Gogniashvili M., Pipia I., Tabidze V., This P., Bacilieri R., Gotsiridze V., Glonti M., 2010. Plastid DNA sequence diversity in a worldwide set of grapevine cultivars (Vitis vinifera L. subsp. vinifera). 10th International Conference on Grapevine Breeding and Genetics. Geneva, 1-5 August 2010.

2011

Ekhvaia, J., F. R. Blattner, M. Gurushidze; M. Akhalkatsi. 2011. Study of genetic diversity and relationships between wild grapevine (Vitis vinifera subsp. sylvestris) populations and aboriginal cultivars in Georgia. Biosystematics conference, 21-27 February, 2011, Berlin, Germany.

Publications in general magazines for growers, public and stake holders.

2007

Bacilieri R. 2007. GrapeGen06-management and conservation of grapevine GR. Bioversity Newsletter for Europe No. 34, Bioversity, Rome. p. 16..

2009

Eiras-Dias, J.E., J. Cunha, Geni Zanol, J. Brazão, 2009. Rede europeia de conservação da videira. Frutas, Legumes e Flores (104), 22-23.

2010

Bucchetti B, Marrazzo MT, Cipriani G, Peterlunger E, 2010. “Caratterizzazione genetica di germoplasma viticolo autoctono del Friuli”. Poster , IX Giornate Scientifiche SOI, 10-12 March 2010, Firenze. The article is in press to “Italus hortus” journal.

Campara E., Galvani S., Di Vecchi Staraz M., Tornielli G.B., Tosi E., Boselli M., 2011. Recupero e caratterizzazione del vitigno Negretta identificato nel territorio di Molina nell’alta Valpolicella (Verona). Enoforum, VII Edizione. Arezzo, 3-5 maggio. In press.

Gardiman M., Borgo M., Giust M. (2010). Vite. In: Piotto B., Giacanelli V., Ercole S. (A cura di), 2010. La conservazione ex situ della biodiversità delle specie vegetali spontanee e coltivate in Italia. Stato dell’arte, criticità e azioni da compiere. Manuali e linee guida ISPRA 54/2010. 63-65.

Righetti S., Boselli M., 2011. Caratterizzazione genetica e attitudini produttive della varietà Righetti (già Arcé) reperita nella Valpolicella (provincia di Verona). VQ, settembre. In press.

Dissemination to growers, general public & stakeholders (genetic resources)

2007

Dekanosidze T., Maghradze D., 2008. Georgian germplasm of grapevine – the national thesaurus. Newspaper “24 hours”. Tbilisi. The 1th March. No 48 (1792). Pp.A6. (in Georgian )

2008


Pospíšilová, D., Sekera, D. Šimora, R.: Dávno pestované odrody viniča na Slovensku (Old grapevine varieties cultivated in Slovakia) Genofond - Spravodajca (Reporter). 2008/12 - in print

2009

Pejic I., Maul E., This P., 2009. New Achievements in Assessing Grapevine Diversity – Impact on Wine Producers, Consumers and Modern Breeding. 32nd OIV World Congress of Vine and Wine – Zagreb (June 28 – July 3, 2009)

2010

Bacilieri R., Maghradze D., Grando S., Pejic I., Maul E., Munoz G., Eiras-Dias J., Schneider A., Boselli M., This P. 2010. Conservation, characterisation and management of grapevine genetic resources : the European project GrapeGen06. 33rd World Congress of Vine and Wine and 8th General Assembly of the OIV. Tbilisi, June 20-27 2010.

Campara E., Galvani S., Di Vecchi Staraz M., Tornielli G.B., Tosi E., Boselli M., 2011. Recupero e caratterizzazione del vitigno Negretta identificato nel territorio di Molina nell’alta Valpolicella (Verona). Enoforum, VII Edizione. Arezzo, 3-5 maggio..

Die Europäische Vitis Datenbank – eine technische Innovation für die langfristige Erhaltung der genetischen Ressourcen der Rebe. Deutsches Weinbaujahrbuch 2012, Maul and GrapeGen06 partners 2011, submitted.

Ekhvaia J, Blattner FR, Akhalkatsi M, 2010. Genetic diversity and relationships between wild grapevine Vitis vinifera L. subsp. sylvestris populations and aboriginal cultivars in Georgia. 33rd World Congress of Vine and Wine and 8th General Assembly of the OIV. Tbilisi, June 20-27 2010.

Gardiman M., Giust M., Carraro R. (2010). Attività di conservazione e caratterizzazione della biodiversità viticola presso il CRA-Centro di ricerca per la viticoltura. Abstract Convegno “BIOD 2010 Coltivare la biodiversità”, Milano 10 – 11 giugno 2010.

Maghradze D., Failla O., Bacilieri R., Imazio S., Vashakidze L., Chipashvili R., Mdinaradze I., Chkhartishvili N., This P., Scienza A.. 2010. Georgian Vitis germplasm: usage, conservation and investigation. 33rd World Congress of Vine and Wine and 8th General Assembly of the OIV. Tbilisi, June 20-27 2010.

Maul E, Ngurah Sudharma K, Müller C, Marx G, Kecke S, Grando S, Pejic I, Munoz G, Eiras Dias J, Schneider A, Boselli M, Bacilieri R, This P., 2010. Die Europäische Vitis Datenbank – eine technische Innovation für die langfristige Erhaltung der genetischen Ressourcen der Rebe. Submitted to : Deutsches Weinbaujahrbuch 2011.

Peterlunger E., 2010. Il progetto europeo GRAPEGEN06: le ricerche svolte dall’Università di Udine sul germoplasma viticolo del Friuli. Oral communication, AGRIEST, 27-30 January 2011.

The European Vitis Database - online uploading and interactive modification system. Bioversity International Newsletter, E. Maul, submitted

Wine tasting were organized by the following partners at their institutes


P01: Public tasting at Weintage Südliche Weinstraße

P02: research institute panel

P02: research institute panel

P07: research institute panel (wine experts)

P10: Public wine tasting with growers at Enoforum

P15: Research institute panel

Finally additional papers are also still in preparation and should be published in the near future:

Pejic et al, on the analysis of molecular data

Schneider et al, : a paper titled “An European cooperative approach to grape varieties identification” describing methodology developed and results achieved under GrapeGen06 on the identification of accessions considered “questionables” is in preparation.

This et al, on the management plan

Conclusion

The important data set is now available online for users (wine growers, viticulturists, scientists, regulators, etc.). The production of publications is in full force in 2011, now that quality checked data are available. More dissemination will be realised in 2012

AGRI GEN RES 0.. - Acronym: …. AGRI-2006- Part C - 61 / 123

7.Overviewofalldeliverablesfromtheproject

Deliverable

number WP

Planned completion

Description

Status

Achieved On going* Remarks

D 01 WP1 Month 04 List of cultivars to be analyzed in WP1 Month 7

D 02 WP1 Month 04 List of SSR-markers necessary for analysis Month 4

D 03 WP1 Month 10 Software for automatic coding Month 7

D 04 WP1 Month 13 List of reference cultivars and alleles for new cultivars Month 18

D 05 WP1 Month 48 Publication in database of coded data for all cultivars End of

project*

D 06 WP2 Month 04 List of a new set of analysis for phytosanitary test (viruses). Month 4

D 07 WP2 Month 04 List of old, endangered and autochthonous varieties to be analysed in WP2

Month 12

D 09 WP2 Month 48 Primary and secondary descriptors data and pictures available via the EU-Vitas-database

Month 46

D 10 WP3 Month 04 Protocol for evaluation of the different characteristics for on-farm analysis

Month 6

D 11 WP3 Month 04 List of vineyards and the cultivars to be analyzed within WP3 Month 10

D 13 WP3 Month 46 Description of the agronomic features of the analyzed varieties / clones (final data)

End of project

D 14 WP3 Month 48 Description of the sanitary status of the analyzed varieties / clones

End of project

D 15 WP3 Month 48 Publication of the results in wine growers magazines to promote the use of rare varieties and outstanding clones of varieties which are still in use.

End of project


D 16 WP4 Month 04 Protocol for the inventory of V. sylvestris Month 5

D 17 WP4 Month 13 First list of sites with Vitis sylvestris plants in Europe and their status regarding their protection

Month 13

D 20 WP4 Month 36 Description of the agronomic features of the analyzed populations (intermediary data) WP4

Month 36

D 21 WP4 Month 46 Description of the agronomic features of the analyzed populations (intermediary data)

End of project

D 22 WP4 Month 48 Final list of sites with Vitis sylvestris plants in Europe and their status regarding their protection

End of project

D 23 WP4 Month 48 Protocol for identification of Vitis sylvestris in Europe Month 6

D 24 WP4 Month 48 SSR data for the V. silvestris populations End of project

D 27 WP5 Month 36 Extended list of cultivars with questionable status and their true status

Month 36

D 28 WP5 Month 46 List of synonyms, proper definition of homonyms “analysed” in collection in Europe

End of project

D 29- WP5 Month 46 Genotypes for true-to-type cultivars (= defined allelic pattern of varieties)

End of project

D 30 WP5 Month 46 List of identified local old, autochthonous cultivars maintained in collections in Europe

End of project

D 31 All Month 13 Data from year 1 for all WP published via the EU-Vitis-database Month 32

D 33 All Month 36 Data published via the EU-Vitis-database (http://www.genres.de/eccdb/vitis) Month 36

D 34 WP5 Month 48 Publication of a table summarizing the entered data subdivided by work packages via the EU-Vitis-database (http://www.genres.de/eccdb/vitis)

End of project

D 35 WP6 Month 48 Dissemination of the results via wine growers magazines End of project

* will be continued after the project end

D 36 WP6 Month 48 Publication of the meeting reports End of project

D 37 WP6 Month 48 Publication of all data via the EU-Vitis-database (http://www.genres.de/eccdb/vitis)

End of project


D 38 WP7 Month 13 Protocoles for cryopreservation of pollen and seeds Month 4

D 39 WP7 Month 36 Protocols for cryopreservation of pollen and seeds Month 24

D 40 WP7 Month 36 1st list of Vitis sylvestris accessions transferred in cryoconservation

Month 36

D 41 Wp4 Month 36 1st list of old autochthonous cultivars transferred in on-farm conservation

Month 36

D 42 WP7 Month 36 1st list of old autochthonous cultivars and Vitis sylvestris accessions transferred in collection

Month 36

D 43 WP7 Month 48 Final list of Vitis sylvestris accessions transferred in cryoconservation

End of project

D 44 WP7 Month 48 Final list of old autochthonous cultivars transferred in on-farm conservation

End of project

D 45 WP7 Month 48 Final list old autochthonous cultivars and Vitis sylvestris accessions transferred in collection

End of project

D 46 WP7 Month 48 List of endangered material in the European collections End of project

* end of project means that the data is available on the version of the database of September 2011.


8.Overviewofallmilestonesfortheproject

Milestone number

WP Planned

completion Description

Status

Achieved On going Remarks

ALL Month 12

Registration of passport data of collections, on-farm víneyards and in-situ sites of Vitis sylvestris in the EU-Vitis- database as well as the registration of SSR-marker data and the installation of the coding and identification program based on SSR-markers

Month 13

M 1 WP1 Month 04 Definition of the list of SSR markers and cultivars to analysed by SSR markers

Month 4

M2 WP1 Month 12 Choice of the cultivars and comparison of data between laboratories for reference alleles Month 13

M3 WP1 Month 12 Coding of SSR data and transfer to WP6 (for available data) Month 18




M07 WP1 Month 48 Publication of complete data in the database Month 48

M 08 WP2 Month 04 Harmonisation and centralization of already existing data available from WP2 Month 18

M 09 WP2 Month 12 Shipment of Primary, secondary descriptors data and pictures data to the coordinator

Month 13


Month 25


All years


M 12 WP2 Month 48 Publication of the descriptor data via the European Vitis Database. End of project

M 13 WP2 Month 48 Catalogue of autochthonous and endangered varieties in Europe. End of project

M 14 WP3 Month 04 Definition of the protocols to be followed for WP3 Month 12

M 15 WP3 Month 12 Inventory of vineyards suitable for analysis and registration of location characteristics

Month 13

M 16 WP3 Month 24 Shipment of agronomic features, wine quality, virus infestation to the coordinator of WP6

Month 25


Month 36


End of project

M 19 WP3 Month 48 Publication of all data in the database End of project

M 20 WP4 Month 12 Inventory of sites in Europe Month 12

M 21 WP4 Month 24 Shipment of morphological and agronomical data for the wild population to coordinator of WP6

Month 24

M 22 WP4 Month 36 Shipment of morphological and agronomical data for the wild population to coordinator of WP6

Month 24

M 23 WP4 Month 46 Shipment of SSR, morphological and agronomical data to the coordinator of WP6

Month 46

M 24 WP4 Month 48 Publication of protocol for identification of V. sylvestris End of project

M 25 WP5 Month 04 Definition of the list of questionable cultivars to be verified in Year 1 Month 12

M 26 WP5 Month 12 Shipment of data recorded for year 1 on questionable cultivars Month 12






M 31 WP5 Month 46 Shipment of data recorded on questionable cultivars End of project

M 32 WP5 Month 46 Analysis of significant citations reported in literature and on database about questionable or old autochthonous cultivars

End of project

M 33 WP6 Month 12 Registration of primary and secondary data and photos of old rare autochthonous varieties / accessions / clones and registration of true-to type SSR-marker data in the EU-Vitis- database

Month 18


Month 24


Month 36

M 36 WP6 Month 48 Registration of primary and secondary data and pictures of old rare autochthonous varieties / accessions / clones and registration of true-to type SSR-marker data in the EU-Vitis- database

End of project

M 38 WP7 Month 36 Collection of pollen and seeds for cryopreservation and shipment of list to WP6

Month 36

M 39 WP7 Month 36 Collection of wood for transfer in collection and shipment of list to WP6 Month 46

M 42 WP7 Month 48 Definition of the plan for duplication of the endangered germplasm in Europe

End of project

M 41 WP7 Month 48 Collection of wood for transfer in collection and shipment of list to WP6 End of project

M 40 WP7 Month 48 Collection of pollen, seeds and buds for cryopreservation and shipment of list to WP6

End of project

___________________________________________________________________________


9.ANNEXES


ANNEX 0 : Results of the coding for the 9 markers.

VVS2 VVS2 VVMD5 VVMD5 VVMD7 VVMD7 VVMD27 VVMD27 VVMD25 VVMD25 VVMD28 VVMD28 VVMD32 VVMD32 VrZAG62 VrZAG62 VrZAG79

VrZAG79 120 N 219 N 231 N 172 N 234 N 214 N 234 N 173 N-1 238 N 122 N+2 223 N+4 233 N+2 176 N+4 236 N+2 216 N+2 236 N+2 174 N 240 N+2 124 N+4 225 N+6 235 N+4 178 N+6 237 N+3 218 N+4 237 N+3 178 N+4 244 N+6 126 N+6 227 N+8 237 N+6 180 N+8 238 N+4 224 N+10 239 N+5 180 N+6 246 N+8 128 N+8 229 N+10 239 N+8 182 N+10 240 N+6 227 N+12 243 N+9 184 N+10 248 N+10 131 N+10 232 N+12 243 N+12 183 N+11 242 N+8 229 N+14 244 N+10 186 N+12 250 N+12 133 N+12 234 N+14 245 N+14 184 N+12 244 N+10 233 N+18 245 N+11 188 N+14 252 N+14 135 N+14 236 N+16 247 N+16 186 N+14 246 N+12 235 N+20 249 N+15 190 N+16 254 N+16 137 N+16 238 N+18 249 N+18 188 N+16 248 N+14 239 N+24 251 N+17 191 N+17 256 N+18 139 N+18 242 N+22 251 N+20 190 N+18 249 N+15 241 N+26 253 N+19 192 N+18 258 N+20 141 N+20 243 N+23 253 N+22 191 N+19 250 N+16 243 N+28 255 N+21 194 N+20 260 N+22 143 N+22 244 N+24 255 N+24 192 N+20 252 N+18 245 N+30 257 N+23 196 N+22 262 N+24 145 N+24 250 N+30 257 N+26 194 N+22 254 N+20 247 N+32 259 N+25 198 N+24 264 N+26 147 N+26 253 N+34 259 N+28 196 N+24 255 N+21 249 N+34 261 N+27 200 N+26 149 N+28 259 N+40 260 N+30 198 N+26 256 N+22 251 N+36 263 N+29 202 N+28 151 N+30 261 N+42 262 N+32 200 N+28 258 N+24 253 N+38 271 N+37 204 N+30 153 N+32 263 N+44 264 N+34 202 N+30 262 N+28 257 N+42 273 N+39 210 N+36 155 N+34 265 N+46 204 N+32 266 N+32 259 N+44 214 N+40 159 N+38 206 N+34 269 N+35 267 N+52 220 N+46

208 N+36 270 N+36 270 N+ 53 210 N+38 276 N+56 212 N+40 214 N+42 216 N+44


ANNEX 1: SSR-marker data done within the project for WP1, Wp4 and WP5 (by July 2011)

Wiews code

Partner Collection

WP1 WP4 WP5 Total size

MCPD data N1 nb

expected%²

old data provided

coded data in database

Total %3

in databa

se

nb genotyped accessions

expected

FRA139 partner00 7502 384 384 100 214 598 573 104 112 43 753

DEU098 partner01 3466 382 384 99 101 483 484 100 8 38 529

AUT024 partner02 927 235 200 118 26 261 198 132 1 262

ESP080 partner03 3416 172 190 91 172 367 47 96 40 308

GRC010 partner04 807 18 100 18 18 77 23 18

PRT051 partner05 647 130 200 65 130 200 65 130

ITA388 partner06 3186 52 48 108 48 100 99 101 59 159

ITA360 partner07 479 140 40 350 140 96 146 30 64 234

ITA362 partner08 1564 147 200 74 147 192 77 25 172

ITA368 partner09 67 28 63 44 28 124 23 32 60

ITAXX01 partner10 94 0 99 0 0 99 0 0

HUN045 partner11 1096 142 - 142 0 142

HUN005 partner12 - 76 150 51 76 150 51 76

CZE041 partner13 55 82 178 46 82 178 46 82

FRA274 partner14 974 794 218 364 185 979 403 243 32 1011

CYP001 partner15 63 0 0 0 0 0

SVK042 partner16 151 0 33 0 0 33 0 0 0

MDA004 partner18 421 0 - 0 0 0

AZE015 partner19 MD 0 - 0 0 0

HRV041 partner20 100 100 100 100 100 124 81 100

GEO013 partner22 - 0 - 0 0 0


GEO014 partner23 147 0 104 0 0 150 0 23* 0

MARXX01

partner24 94 94 94 100 94 94 100 94

ITA41 C. d'Onofrio 15 0 15 15 100 15

DE494 A. Jung 0 0 0 0 0

ITA035 + GEOXX01

S. Imazio 156 17 1053 156 20 895 179

CSIC C. Martinez 0 0 0 0 0

Turkey S. Erciszli 0 0 0 0 0

Total 25 583 3 147 2 802 113 574 3 721 3 676 102 269 333 4 323 1 = Number of accessions analysed within grapeGen06 project 2 = Proportion of analysis performed within grapeGen06 project compared to expected ones 3= Proportion of total number of analysis uploaded in database compared to expected ones *= labelled WP1 and uploaded by Italian colleague under nb ITA035-158 to 180

AGRI GEN RES 008. Acronym: Grapegen06 AGRI-2006. Part A - Annexes 71 / 123

ANNEX 2 – WP1. Methodology and concepts for data analysis

The expected outcomes of the WP1 (publishable results): 1. Usability of the 9 selected SSRs in the management of the V. vinifera germplasm

repositories. Up to date level of SSRs fidelity in decision making when accession have to be determined as synonyms, homonyms and relatives. By using large amount of data (n=2901) and 18 different repositories (if combined with already published data of Laucou et al - TAG, 2011), the GrapeGen06 grapevine molecular dataset would be biggest dataset published so far) we can provide very robust data and conclusions on:

o a proven system for data harmonization by using referent set of grapevine DNAs and its coding methodology, able to deal with the influence of different lab practice, PIC values and reliability of particular SSR markers;

o a proven system for data exchange and comparison among laboratories for identification purposes

o data storage and retrieving capability of an open-access database, offering an easy possibility ensure comparison of single institute data with those stored in the database;

o by contrast, confirmation of the non-usability of SSRs in clone determination. P14 (IFV) provided a huge data set of more than 1000 profiles. More than 800 (>80%) were detected as identical matches, which is a very robust set to confirm inability of SSRs for clone differentiation, as well as good reliability of SSRs when produced in the same lab

2. Reliability of the accessions (true-to-type) from the grapevine repositories. By using

SSR profiles and the several groups of accessions from various collections representing the same cultivars it the current project made it possible to estimate the level of accuracy of accession names. It provided a very reliable estimate of the number of uncertain, rare or duplicated accessions among 18 different collections. This result will greatly improve our knowledge on the current state of the actual ex situ grape diversity, as well as an easy accessibility to the living resources since all accessions are referenced with the holding institution data.

3. Contribution of the newly genotyped GrapeGen06 germplasm to the general

knowledge on grapevine population genetics. The level of diversity (SSR polymorphism) and level of detected synonyms and duplicates in the leading European repositories, as well as contribution of the new ones, might enable revision of total number of cultivars worldwide. The latest and most robust data on number and frequency of 9 SSR alleles, PIC, He, Ho, etc. will improve the general knowledge on grapevine as cultivated species.

Detail of the work (WP1)

1. Each partner data were tested for identical profiles (100% match) and numerous identical genotypes were selected out (mostly clones and synonyms). Selection was performed using „Genetic Analysis in Excel“ macro tool, command „multilocus matches“ (data subset without matches, option: ignore missing data). After the software removed accessions with identical profiles, common data set had 2901 accessions


(different SSR profiles). This data set still a number of synonyms and similar accessions among partners. It has been used for further analyses.

2. This data set (n=2901) has been used to analyze:

a. SSR data consistency (genotyping and coding errors) as reflection to different

lab values, with the purpose to call partners for last data improvement (to repeat scoring or genotyping); - very likely “incorrectly genotyped accessions and wrong alleles” (2%) were marked and sent to owners for revision (or excluded from further analysis)

b. detection of synonyms and very related accessions, i.e. to identify accessions with identical or „very similar“ profiles (differing in up to 2 loci if difference is +/- 2 bp or heterozigous/homozigous);

c. accession name consistency over different partner collections verified by SSR profile, and suggestion of a list of synonyms at the European level for reporting in the database

d. to asses the contribution of the „newly genotyped germplasm“ to the number of new alleles per locus, allele frequencies, core set, etc. (free of false data), as well as to estimate the level of first-degree and parentage relationships (new ones and verification of old ones) – a new (reduced) spreadsheet table with “very reliable” and non redundant (unique profile) data was generated.


ANNEX 3: Summary of the state of advancement for morphological data (data in the database by July 2011)

Wiews code Partner WP2 WP4 WP5

Total Produced within

grapegen06

Virus (WP2)

Pictures

N11 Old Total

done Exp. %

FRA139 partner00 80 111 201 64 144 40 360 71 375 DEU098 partner01 50 220 310 6 32 88 50 176 50 432 AUT024 partner02 23 26 49 23 10 230 104 ESP080 partner03 46 111 522 321 44 411 50 822 45 238 GRC010 partner04 0 0 199 PRT051 partner05 56 116 172 53 109 60 182 399 ITA388 partner06 30 101 169 38 68 10 680 46 327 ITA360 partner07 71 100 233 30 52 153 40 382 91 613 ITA362 partner08 29 100 129 20 49 30 163 105 ITA368 partner09 12 26 57 9 21 30 70 30 220

ITAXX01 partner10 0 0 0 40 0

HUN045 partner11 109 0 109 50 218 207 HUN005 partner12 0 0 - - - CZE041 partner13 30 124 154 30 62 50 35 89 FRA274 partner14 0 0 - - - CYP001 partner15 9 9 9 10 90 76 SVK042 partner16 26 151 137 163 20 815 CHE019 partner17 0 0 0 24 0

MDA004 partner18 0 0 40 0 AZE015 partner19 0 0 35 0 HRV041 partner20 100 100 100 100 100 99 238

GEOXX01 partner21 0 0 0 GEO013 partner22 13 0 13 20 65 GEO014 partner23 25 25 25 50 50 55

MARXX01 partner24 0 0 0 ITA041 C. d'Onofrio 0 0 DE494 A. Jung 0 0 ITA035 S. Imazio 0 0 CSIC C. Martinez 0 0

Turkey S. Erciszli 0 0 Total 709 955 2295 631 175 1515 771 196 467 3678

1 = Number of descriptions performed within grapeGen06 project uploaded in database 2= Proportion of analysis performed compared to expected ones


Annex 3 bis : list of cultivars analysed within Grapegen06 project (WP2) that are virus free 1‐INSTCODE 2‐ACCENUMB 11‐ACCENAME A.Variety Name

CZE041 CZE041‐24V0130047 1527‐1‐EM 1527‐1‐EM

CZE041 CZE041‐24V0130050 1540‐51‐EM 1540 ‐ 51 ‐ EM

CZE041 CZE041‐24V0130051 1624‐42 1624 ‐ 42

CZE041 CZE041‐24V0130052 32‐B‐8 32 ‐ B ‐ 8

ITA360 ITA360‐516 Acini piccoli Trevisana nera

CZE041 CZE041‐24V0130003 Auxerrois Auxerrois

ITA360 ITA360‐163 Avarengo Avarengo

ITA360 ITA360‐605 Baratuciàt Baratuciàt

ITA360 ITA360‐334 Barbarossa 6 Barbarossa

ITA360 ITA360‐179 Barbera 'd Davi Barbera 'd Davi

DEU098 DEU098‐1998‐100 BATTTRAUBE

ITA360 ITA360‐543 Beuma Mollard

ITA360 ITA360‐465 Biauna Doux d'Henry

ITA360 ITA360‐476 Bibiola Bibiola

ITA360 ITA360‐641 Biona

DEU098 DEU098‐1998‐101 BLUSSARD SPAET BLAU GAMAY NOIR

ITA388p ITA388p‐stAN10 BOTTAIO NERO AN10

ITA360 ITA360‐090 Brachetto Migliardi Brachetto (Migliardi)

CZE041 CZE041‐24V0130826 Bratislavské bílé Bratislavské bílé

HRV041 HRV041‐SA#11‐01 Bratkovina crvena Bratkovina crvena

ITA360 ITA360‐206 Bubbierasco

ITA360 ITA360‐518 Bubia

CZE041 CZE041‐24V0130007 C‐3‐53 C ‐ 3 ‐ 53

ITA360 ITA360‐487 Carcairone bianco Hibou blanc

ESP080 ESP080‐BGVCAM1575 CARRASQUÍN CARRASQUÍN

CZE041 CZE041‐24V0100175 Ceaus x Bolgar 2 Ceaus x Bolgar 2

CZE041 CZE041‐24V0100275 Cerven septenvrijski Cerven septenvrijski

ITA388 ITA388‐757 COLORINO AMERICANO

ITA368 ITA368#SANOSVALDO/V7_13‐34.38

CORVINO NIMIS Corvino

CZE041 CZE041‐24V0130008 CSE‐13 CSE ‐ 13

CZE041 CZE041‐24V0130009 CSE‐15 CSE ‐ 15

ESP080 ESP080‐BGVCAM0892 DE REY DE REY

ITA360 ITA360‐633 DiRovasenda 7/2/4 Nebbiolo

ITA360 ITA360‐171 Doux d'Henry Doux d'Henry

ITA360 ITA360‐526 Durasa Durasa

CZE041 CZE041‐24V0130810 Efremovskii rannii Efremovskii rannii

ITA360 ITA360‐060 Erbaluce nero Durasa

CZE041 CZE041‐24V0100949 Faber Faber

ESP080 ESP080‐BGVCAM0971 FERNANDELLA FERNANDELLA

ESP080 ESP080‐BGVCAM1132 FOGONEU FOGONEU

CZE041 CZE041‐24V0130017 Fontanara Fontanara


ITA360 ITA360‐189 Galletta

ESP080 ESP080‐BGVCAM1134 GIRÓ GIRÓ

ITA360 ITA360‐234 Grandurey

ITA360 ITA360‐152 Grisa di Cumiana Grisa nera

ITA360 ITA360‐506 Grisa nera falsa 1 Pelaverga

CZE041 CZE041‐24V0100374 Gutenborner Gutenborner

CZE041 CZE041‐24V0130616 Hedvábné zluté Hedvábné zluté

HRV041 HRV041‐SA#11‐94 Jarbola Jarbola

ESP080 ESP080‐BGVCAM1543 JAUMES BEBA

CZE041 CZE041‐24V0100233 Julski biser Julski biser

DEU098 DEU098‐1998‐111 JUNGFERLEN

CZE041 CZE041‐24V0130022 Kerner Kerner

HRV041 HRV041‐SA#11‐21 Kraljevina Kraljevina

HRV041 HRV041‐SA#11‐22 Krivaja crvena Krivaja crvena

HRV041 HRV041‐SA#11‐25 Kurtelaska Kurtelaska

ITA360 ITA360‐164 Lambrusca vittona Lambrusca vittona

CZE041 CZE041‐24V0100185 Leanka Leanka

HRV041 HRV041‐SA#12‐00 Lipovina Lipovina

ITA360 ITA360‐501 Luglienga Lignan

CZE041 CZE041‐24V0130026 Magarac 360 Magarac 360

ITA388p ITA388p‐stA14CUB MAMMOLO A14CUB

ESP080 ESP080‐BGVCAM1145 MANTO NEGRO MANTO NEGRO

ITA360 ITA360‐596 Maria lunga Dorona veneziana

CZE041 CZE041‐24V0130027 Mariensteiner Mariensteiner

FRA139 FRA139‐188Mtp1 Mècle Mècle

CZE041 CZE041‐24V0130028 MJ x KO 44/2 MJ x KO 44/2

ESP080 ESP080‐BGVCAM1921 MORENILLO MANDÓN

FRA139 FRA139‐0Mtp739 Morenoa Morenoa

ITA360 ITA360‐535 Moretto

ITA360 ITA360‐249 Moscato nero Moscato nero di Acqui

HRV041 HRV041‐SA#11‐32 Moslavac Furmint

ITA360 ITA360‐182 Muliné

ITA360 ITA360‐187 Neirano di Spagna

ITA360 ITA360‐126 Neretto di S.Giorgio Neretto di Bairo

ITA360p ITA360p‐on‐farm‐Neretto duro_2 Neretto duro

ITA360p ITA360p‐on‐farm‐Neretto duro_1 Neretto duro

ITA368 ITA368#SANOSVALDO/V7_14‐4.8 NERO BUIA

CZE041 CZE041‐24V0130032 Nobling Nobling

ITA360p ITA360p‐Q001 Obi

CZE041 CZE041‐24V0130818 Osobyj Osobyi

FRA139 FRA139‐426Mtp1 Pé de Perdrix Pé de Perdrix

ESP080 ESP080‐BGVCAM1985 PEDRO LUIS PEDRO LUIS

ITA388 ITA388‐2127 PEDRO XIMENES 01 PEDRO XIMENES

FRA139 FRA139‐1232Mtp3 Pignon Gros Cabernet

HRV041 HRV041‐SA#11‐40 Plavac mali crni Plavac mali crni


HRV041 HRV041‐SA#11‐45 Plavina Plavina

ESP080 ESP080‐BGVCAM0912 PORTUARTO PUERTO ALTO

HRV041 HRV041‐SA#11‐51 Prsljivka Prsljivka

ITA360 ITA360‐180 Quagliano Quagliano

CZE041 CZE041‐24V0130041 Reichensteiner Reichensteineir

ESP080 ESP080‐BGVCAM1052 REY REY

ITA368 ITA368#SANOSVALDO/V7_9‐4.8 RIBOLLA SPIZADE

ITA360 ITA360‐407 Rossese bianco (Roddino) Rossese bianco

FRA139 FRA139‐0Mtp988 Rossola bianca Rossola bianca

CZE041 CZE041‐24V0100708 Ryzlink aromatický Ryzlink aromatický

CZE041 CZE041‐24V0130629 Ryzlink cervený Ryzlink cervený

CZE041 CZE041‐24V0100193 Ryzlink rýnský Ryzlink rýnský

FRA139 FRA139‐203Mtp1 Saint Laurent Saint‐Laurent

ESP080 ESP080‐BGVCAM1895 SALVADOR SALVADOR

ITA388p ITA388p‐stF16 SANVICETRINO F16

ITA368 ITA368#SANOSVALDO/V7_13‐24.28

SCALA SANTA 4

ITA360 ITA360‐225 Sconosciuta Pinasca Raboso veronese

ITA360 ITA360‐639 Sconosciuta Spineto

HRV041 HRV041‐SA#11‐59 Sipelj Sipelj

ITA360p ITA360p‐on‐farm‐Slarina_2 Slarina Slarina

ITA360p ITA360p‐on‐farm‐Slarina_1 Slarina Slarina

ESP080 ESP080‐BGVCAM1988 SOUSÓN SOUSÓN

HRV041 HRV041‐SA#11‐56 Stara brancevka Stara brancevka

ESP080 ESP080‐BGVCAM1168 SUMOLL SUMOLL

ESP080 ESP080‐BGVCAM1588 TARANTEY TORRONTÉS (Galicia) (BOAL CACH

ESP080 ESP080‐BGVCAM1837 TARDANA PLANTA NOVA

ESP080 ESP080‐BGVCAM1288 TARRAGONÍ TARRAGONÍ

CZE041 CZE041‐24V0101067 TC x VCR 7/6 Záhoranka

ESP080 ESP080‐BGVCAM1589 TINTA FEMIA DE ALDÁN BENEDICTO

ITA360 ITA360‐027 Tinturié (acino rotondo) Teinturier ad acino rotondo

ITA360 ITA360‐591 Uva 'Alberti'

ITA388 ITA388‐2972 UVA ACETO

ITA360 ITA360‐351 Uva del re Kharistvala kolkhuri

ITA360 ITA360‐109 Uva delle cascine

ESP080 ESP080‐BGVCAM1896 VERDIL VERDIL

ITA360 ITA360‐530 Vermeju Moradella

ITA368 ITA368#SANOSVALDO/VC_10 VITOUSKA

HRV041 HRV041‐SA#11‐68 Vranac Vranac

HRV041 HRV041‐SA#11‐70 Vugava bijela Vugava bijela

HRV041 HRV041‐SA#11‐72 Zlatarica blatska Zlatarica blatska


ANNEX 4: List of the cultivars selected by the 12 partners participating at the WP3.

Partner

nber Institute Cvr name

SSRVineyards number

Characte-rization years*

vine making year

Degus-tation year

Virus status

Indemne (I) or

Infected for

1 BAZ -D Heunisch Y 2 2008/2010 2009 2009 y I

1 BAZ -D Elblich blau Y 2 2008/2010 2009 2009 y I

2 HBWO - A

Österreichisch Weiß

Y 1 2008/2010 2008/2010 2010

Y GlRa1v

3 IMIDRA - E Albillo y 2 2008/2009 2009/2010 2010 y I

3 IMIDRA - E Malvar Y 4 2008/2009 2009/2010 2010 Y I

5 EVN- INIAP- P Verdelho B Y 2 2009 2009 N

5 EVN- INIAP- P Galego Dourado B Y 2 2009 2009 N

5 EVN- INIAP- P Malvasia B Y 2 2009 2009 N

5 EVN- INIAP- P Ramisco N Y 2 2009 2009 N

5 EVN- INIAP- P Molar N Y 2 2009 2009 N

6 ISV-Sam-I Aghedene N 1 2007/2008 ? N

6 ISV-Sam-I Barsaglina N 1 2007 ? N

6 ISV-Sam-I Berzamino N 1 2006-2008 ? N

6 ISV-Sam-I Brambana N 1 2006-2008 ? N

6 ISV-Sam-I Capolongo N 1 2004-2007 ? N

6 ISV-Sam-I Cjavalgjàn N 1 2006-2008 ? N

6 ISV-Sam-I Crepolino N 1 2007 ? N

6 ISV-Sam-I Cuneute 1 2006-2007 ? N

6 ISV-Sam-I Fumat 1 2005-2008 ? N

6 ISV-Sam-I Lacrima del Valdarno

1

2003-2008 ? N

6 ISV-Sam-I Lecinaro 1 2004-2007 ? N

6 ISV-Sam-I Maturano bianco 1 2004-2007 ? N

6 ISV-Sam-I Morellone del 1 2007 ? N


Casentino

6 ISV-Sam-I Negrat 1 2005-2007 ? N

6 ISV-Sam-I Orpicchio 1 2007 ? N

6 ISV-Sam-I Pampanaro 1 2004-2007 ? N

6 ISV-Sam-I Rosciola 1 2004-2007 ? N

6 ISV-Sam-I Sagrestana 1 2005-2008 ? N

7 CNR-IVV-I Montanera (di Perosa)

Y 1 2008/2009

2008/2009 Y I

7 CNR-IVV-I Neretto duro Y 2 2008/2009 2008/2009 Y I

7 CNR-IVV-I Slarina Y 2 2008/2009 2008/2009 Y I

8 IASMA-I Casetta Y

1 1995-

2006/2008-2009 2008/2009

2008/2009

N

8 IASMA-I Rossara Y

1 1995-

2006/2008-2009 2008/2009

2008/2009

N

8 IASMA-I Verdealbara Y

1 1995-

2006/2008-2009 2008/2009

2008/2009

N

9 DISAA – UNIUD Berzamin 2 ? 2005 N

9 DISAA – UNIUD Cianorie 2 ? 2005 N

9 DISAA – UNIUD Cividino 2 ? 2005 N

9 DISAA – UNIUD Fumat 2 ? 2006 N

10 UNIVR Arcè n 2 2007-2009 2009 Y I

10 UNIVR Negretta 2 2007-2009 2009 Y I

10 UNIVR Terodola N 2 2007-2009 2009 Y I

11 FVM Kadarka 2009 2009 2009 N

13 RICP- CZ Jakubskè Y 2 2009 2008/2009 Y I

13 RICP- CZ Tramin Bily Y 2 2009 2008/2009 Y I

14 IFV Milgranet 1 2009 2008/2009 2009 N

14 IFV Moussaygues

1

2007 (WP1)-2009 2008/2009 2009

N


14 IFV Negretta 1 2009 2008/2009 2009 N

14 IFV Prunelard 1 2009 2008/2009 2009 N

15 ARI -CY Spourtiko 1 (2007-2009 ? N

15 ARI -CY Yiannoudhi 1 (2007-2009 ? N

20 DVE- Univ Zagreb Babica N 6 2005/2008 ? N

20 DVE- Univ Zagreb Brajdica bijela N 6 2007/2008 ? N

20 DVE- Univ Zagreb Glavinuša 6 2005/2008 ? N

20 DVE- Univ Zagreb Ljutun 6 2005/2008 ? N

20 DVE- Univ Zagreb Mladenka 6 2005/2008 ? N

20 DVE- Univ Zagreb Ninčuša 6 2005/2008 ? N

20 DVE- Univ Zagreb Sansigot 6 2006/2007 ? N

20 DVE- Univ Zagreb Vlaška 2005/2008 ? N

20 DVE- Univ Zagreb Zumic

2006/2007 ?

N

S

S

c

AGRI GE

ANNEX 5:these 2 cu

Screen 1 : p

Screen 2 : p

characterize

EN RES 0.. - Acr

: Screenshoultivars, in

presentatio

presentatio

ed only by a

ronym: ….

ots for 2 cuparticular

on of the cu

on of the ag

ampelograp

ultivars anathe data on

glob

ultivar and

gronomic f

hy

alysed within choice of

bal surface.

Vineyards

features fo

AGRI-2

in WP3, shf the cultiva.

s for Aghed

r Agheden

2006-…. Part A

howing the ar, historica

dene

e : this cult

A –Annexes 80

data recordal data and

tivar has be

0 / 123

ded for d area

een

S

S

AGRI GE

Screen 3 : p

Screen 4 : p

EN RES 0.. - Acr

presentatio

presentatio

ronym: ….

on of the am

on of the cu

mpelograp

ultivar and

phy data fo

Vineyards

AGRI-2

r Agheden

s for Elbling

2006-…. Part A

e

g blau

A –Annexes 81

1 / 123

S

c

AGRI GE

Screen 5 : p

cultivar has

EN RES 0.. - Acr

presentatio

been chara

ronym: ….

on of the am

acterized bo

mpleograp

oth by ampe

phy and agr

elography a

AGRI-2

ronomic fe

nd SSR

2006-…. Part A

eatures for

A –Annexes 82

Elbling bla

2 / 123

au : this

AGRI GEEN RES 0.. - Acr

ANNE

ronym: ….

EX 6: Exam

mple of agro

onomical d

AGRI-2

data for sev

2006-…. Part A

eral cultiva

A –Annexes 83

ars

3 / 123

AGRI GEEN RES 0.. - Acr

AN

ronym: ….

NNEX 7: E

Example of

wine data

AGRI-2

for several

2006-…. Part A

cultivars

A –Annexes 844 / 123

ANNEX 8 : F

AGRI GEN R

Frequencies of e

RES 0.. - Acronym: ….

each alleles at e

.

each of the 9 loc

cus for both the

AGRI-2006-…. Part

e cultivars (CV)

A –Annexes 85 / 123

) and the wild in

3

ndividuals (Lammb)

AGRI GEN RES 0.. - Acronym: …. AGRI-2006-…. Part A –Annexes 86 / 123

ANNEX 9: Multivariate analysis of the samples. Analysis based on the 9 SSR: From the 2772 unique genotype, 2239 with no missing data were selected and analysed (in red the

cultivars, in bleu the wild individuals).

Factorial analysis: Axes 1 / 2

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AGRI GEN RES 0.. - Acronym: …. AGRI-2006-…. Part A –Annexes 87 / 123

ANNEX 10 - WP5 The contribution of partners and the results of the analysis of "questionables" varieties.

Partners N. of submitted questionables

Participation to the

identification (2010) N. of

questionables Identified

N. of questionables

requiring more investigation

N. of questionables un-identified

% Identified

% More investigation

% Un-identified

0 FRA139 INRA, France 46 X 9 6 31 19,6 13,0 67,4

1 DEU098 BAZ – IRZ, Germany 38 X 20 14 4 52,6 36,8 10,5

2 AUT024 HBLAuBA, Austria 26 X 4 2 20 15,4 7,7 76,9

3 ESP080 IMIDRA, Spain 42 X 21 2 19 50,0 4,8 45,2

5 PRT051 EVN - INIAP, Portugal 20 3 8 9 15,0 40,0 45,0

6 ITA388 CRA-ISV, Italy 71 X 25 7 39 35,2 9,9 54,9

7 ITA360 CNR-IVV, Italy 52 X 18 0 34 34,6 0,0 65,4

8 ITA362 IASMA, Italy 28 X 23 3 2 82,1 10,7 7,1

9 ITA368 DISAA UNiUD, Italy 29 X 17 3 9 58,6 10,3 31,0

13 HUN005 SIU DGPB, Hungary 2 1 1 0 50,0 50,0 0,0

14 CZE041 RICP, Czech Republic 13 2 0 11 15,4 0,0 84,6

15 FRA274 ENTAV, France 21 X 16 4 1 76,2 19,0 4,8

20 HRV041 FAZ, Croatia 1 X 1 0 0 100,0 0,0 0,0

Jung, Germany 61 17 16 28 27,9 26,2 45,9

Tot 14 450 10 177 66 207 39,3 14,7 46,0

AGRI GEN RES 008.. - Acronym: Grapegen06 AGRI-2006 Annexes 88/123

ANNEX 11 - WP 5 : What is “trueness to type” and how the concept is developed inside the European Vitis Database?

Vitis vinifera and related species include a very large number of cultivars spread all over the world. Intensive trades (ancient and recent) of grape propagating materials, the wide spreading of this crop through different regions, countries and continents, the development of local uses that link grape varieties to areas, all contributed to the emergence of synonyms (different names used for the same variety), the use of homonyms (same name for different varieties) and the occurrence of errors and misnomers in variety designation (when a variety is wrongly named). Synonyms, homonyms and misnomers are often the source of confusion, misunderstanding, frauds. Hence the definition of what is called “true to type” of a certain variety (i.e. the true, identified form to be used as reference) is of crucial importance. A “true to type” variety (or accession) truly refers to the reference variety, is rather truly identified, and the name of the related accession, when not corresponding to the one of the reference variety, can be an alternative spelling (dialect term), a synonym, or a misnomer when confusingly used for another identified variety. In the European Vitis Database several passport descriptors specifically allow the indication of the true to type status of the variety. When the accession is identified, the “Variety name” is indicated, reporting the name of the reference variety, “Trueness to type” (which refers in this database to the variety name) is “yes” and the descriptor “Remarks to the accession name”, according to the case, can be: dialect term, homonym, misnomer, synonym or true name (in this last case when it precisely corresponds to the name of the reference variety). Therefore, the status “yes” of trueness to type refers to a variety which has been properly identified and correctly named. The corresponding accession can be correctly named or not, according to “Remarks to the accession name”. It is evident that the name of the variety (descriptor “Variety name”) has to be either the official name of the variety (name registered in the National Catalogue) or the local, attested name (documented in historical references) when the variety is not officially registered. In order to link varieties for which synonyms are used in different countries and in different National Catalogues (i.e. Trebbiano toscano ITA, Ugni blanc FRA, Talia PRT) the corresponding number of the variety adopted in the Vitis International Variety Catalogue (http://www.vivc.de/index.php) has been added as passport descriptor (“Variety number VIVC”). A set of further descriptors were conceived in order to indicate whether the identification of an accession with a known reference variety was achieved through: a) “ampelography” (plant morphology), b) “SSR markers” (matching genetic profiles), and c) “bibliography” (historical or actual descriptions, where the precise indication of the literature references are given). If, for example, the identification was achieved through the comparison of the morphological features of the plants with a published ampelographic description, the choice will be “ampelography”, while in “bibliography” the precise literature reference will be given. Finally, several accessions regarded as “questionables” were analyzed under GrapeGen06 with the purpose of their identification. The European Vitis Database envisages curators of the collections indicate the accessions recognized as “questionables” or not. Questionable accessions are those clearly misnamed (i.e. “False Cabernet Sauvignon”), those without a proper name (“Unknown”), or those that, being identified in a certain area (often by local historical references), are checked for their occurrence in other places/countries (perhaps under different names). The “questionables” identified through the analysis carried out in the frame of GrapeGen06 are shown as “identified” in the European Vitis Database.

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Some rules have to be followed for assigning the proper “Variety name”: it should be the official name of the variety reported in at least one National Catalogue when the variety is officially registered; it could be a locally attested name, or a name reported in historical/local documents when the variety is not officially registered because minor, rare, neglected. In order to link varieties for which synonyms are used in different countries and included in different National Catalogues (i.e. Trebbiano toscano ITA = Ugni blanc FRA = Talia PRT) the corresponding number of the variety adopted in the Vitis International Variety Catalogue (http://www.vivc.de/index.php) is used as additional descriptor (“Variety number VIVC”). Finally, “Confirmation by bibliography” can either correspond to an historical reference (if documentation enables identification by comparison), or to a more recent description of the variety to be given as reference: both are often the case for local, minor varieties on which GrapeGen06 is specifically focused.

T

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AGRI-2006 Annexes 9

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1/123

AGRI GEEN RES 008.. - AAcronym: Grapeegen06

AAGRI-2006 Annexes 922/123

AGRI GEEN RES 008.. - AAcronym: Grape

egen06



egen06



egen06



egen06



egen06





egen06






AGRI GEN RES 0.. - Acronym: …. AGRI-2006 Part A : Annexes 102/123

ANNEX 14: Results of the comparison of the grapegen06 database and Partner 7 database.

Sample 1 (GrapeGen06)

11-ACCENAME of sample1

Variety name of sample1

Sample 2 (partner 7, ITA360)

Score N Alleles used

Nb. Alleles Matching

Nb. MD S1

Nb. MD S2

B-BERRY COLOR of sample 1

Trueness to type of the variety

RempartnITA3

FRA139-124Mtp1

Riminese Riminèse Albana 94,44% 18 17 0 0 GREEN yes confiWP5

FRA139-171Mtp2

Hibou Hibou noir Avanà 100,00% 18 18 0 0 BLACK yes alreaknow

FRA139-164Mtp12

Persan (thermo.) Persan Becouet 100,00% 18 18 0 0 BLACK yes alreaknow

FRA139-106Mtp1

Sauvaget Sauvaget noir Berla 'd Crava (di Borgone)

100,00% 18 18 0 0 BLACK yes confiWP5

FRA139-98Mtp8 Ramollon Mollard Beuma 100,00% 18 18 0 0 BLACK yes confiWP5

FRA139-158Mtp5

Verdesse Verdesse Bianc vert 100,00% 18 18 0 0 GREEN yes alreaknow

FRA139-119Mtp2

Carcajolo n°30 Carcajolo blanc Bianca frizzante 94,44% 18 17 0 0 GREEN yes

HRV041-SA#11-64

Topol Topol Bianco d'Alessano 8 88,89% 18 16 0 2 GREEN

ITA362-906 Grecanico Bianco di Pomaretto 94,44% 18 17 0 0 GREEN confiWP5

FRA139-224Mtp6

Verrot moussu Tressot Bizzaria variegata 88,89% 18 16 0 2 BLACK yes alreaknow

ESP080-BGVCAM2155

LEGIRUELA LEGIRUELA Blanc de Morgex 100,00% 18 18 0 0 GREEN yes alreaknow

ITA362-1136 Maiolica Buona qualità 94,44% 18 17 0 0 BLACK true Misn

FRA139-169Mtp5

Martin Cot Hibou blanc Carcairone bianco 94,44% 18 17 0 0 GREEN yes alreaknow


CZE041-24V0100224

Chrupka bílá Chrupka bílá Chasselas blanc 100,00% 18 18 0 0 GREEN yes

CZE041-24V0100225

Chrupka cervená Chrupka cervená Chasselas blanc 100,00% 18 18 0 0 ROSE yes

CZE041-24V0100226

Chrupka Jalabertova

Chrupka Jalabertova

Chasselas blanc 94,44% 18 17 0 0 GREEN not checked

ESP080-BGVCAM1703

TEMPRANO BLANCO

TEMPRANO BLANCO (CHASSELAS DORÉ)

Chasselas blanc 100,00% 18 18 0 0 GREEN yes alreaknow

ESP080-BGVCAM1587

NEGRÓN DE ALDÁN

NEGRÓN DE ALDÁN (PETIT BOUSCHET)

Colorino (di Lucca) 88,89% 18 16 0 2 BLACK yes

FRA139-479Mtp1

Petit Bouschet Petit Bouschet Colorino (di Lucca) 88,89% 18 16 0 2 BLACK uncertain confiWP5

ITA388-2246 PIPIONA PETIT BOUSCHET

Colorino (di Lucca) 88,89% 18 16 0 2 BLACK yes confiWP5

DEU098-1992-069

CORNALIN D'AOSTE

CORNALIN D'AOSTE

Cornalin clone 1 (Humagne rouge)

88,89% 18 16 0 0 BLACK yes alreaknow

FRA274-90817E1

Humagne Rouge N clone 6002

Humagne Rouge N

Cornalin clone 1 (Humagne rouge)

94,44% 18 17 0 0 BLACK yes alreaknow

ITA388-2391 RAMBELLA Famoso 88,89% 18 16 0 2 GREEN alreaknow

ITA388-954 FOGARINA FOGARINA Fogarina RE 88,89% 18 16 0 2 BLACK yes alreaknow

HRV041-SA#11-03

Cipar Grec rouge Grisa rousa 100,00% 18 18 0 0 ROSE alreaknow

AUT024-195 KOTSI FALI Kotsifali 94,44% 18 17 0 0 BLACK GRC005-12/B KOTSIFALI Kotsifali 88,89% 18 16 2 0 BLACK yes GRC005-P06#19-E

ASKATHARI Kotsifali 100,00% 18 18 0 0 GREEN yes MutaCheccolou

HRV041-SA#11- Teran Terrano Lambrusco dal picoll ross 100,00% 18 18 0 0 BLACK alrea


63 RE knowITA388-1982 NEGRETTA Lambrusco

viadanese Lambrusco mantovano RE

94,12% 17 16 0 0 BLACK yes

ITA362-1100 Lambrusco salamino Lambrusco salamino RE 100,00% 18 18 0 0 BLACK DEU098-1993-303

HEUNISCH WEISS

HEUNISCH WEISS

Liseiret 94,44% 18 17 0 0 GREEN yes alreaknow

DEU098-2001-091

VELTLINER GRAU

HEUNISCH WEISS

Liseiret 94,44% 18 17 0 0 GREEN yes alreaknow

HUN045-P14#408

HEUNISCH WEISS Liseiret 88,89% 18 16 0 0 GREEN alreaknow

CZE041-24V0130616

Hedvábné zluté Hedvábné zluté Luglienga bianca (Lignan) 88,89% 18 16 0 0 GREEN not checked

ESP080-BGVCAM1109

BATISTA BATISTA Luverdon 100,00% 18 18 0 0 BLACK yes alreaknow

FRA139-458Mtp10

Gamay malain n°1 Canari Luverdon 100,00% 18 18 0 0 BLACK yes alreaknow

ESP080-BGVCAM1806

MALVASÍA BLANCA Malvasia bianca lunga del Chianti (Grugliasco)

88,89% 18 16 0 2 GREEN not checked

DEU098-1993-231

MALVASIA CANDIDA

MALVASIA CANDIDA

Malvasia di Candia 94,44% 18 17 0 0 GREEN yes

HRV041-SA#12-04

Malvasija dubrovacka

Malvasija dubrovacka

Malvasia Sarda (Malvasia di Lipari)

88,89% 18 16 0 2 GREEN alreaknow

ESP080-BGVCAM1025

MOSCATEL GORDO

MOSCATEL DE ALEJANDRÍA

Moscato di Alessandria 94,44% 18 17 0 0 GREEN yes

GRC005-27/A MOSCHATO OF ALEXANDRIA Moscato di Alessandria 94,44% 18 17 0 0 GREEN yes MARXX01-Sodea-43

muscat doukhala Muscat d'Alexandrie B

Moscato di Alessandria 94,44% 18 17 0 0 no

HUN045-P14#8 ALEXANDRIAI MUSKOTALY Moscato di Alessandria 100,00% 18 18 0 0 GREEN HUN045-P14#225

CSIKOS MUSKOTALY Moscato rosso (mutaz. Bianco)

94,44% 18 17 0 0 GREEN

FRA139-570Mtp11

Orange muscat Muscat fleur d'oranger

Muscadeddu (M. fior arancio)

88,89% 18 16 0 2 GREEN yes alreaknow

ITA362-1328 Muscat Fleur d'Oranger Muscadeddu (M. fior 88,89% 18 16 0 2 GREEN alrea


arancio) knowHRV041-SA#12-08

Muskat ruza omiski

Muskat ruza omiski

Muskat Hambourg 100,00% 18 18 0 0 BLACK confiWP5

FRA139-3019Mtp1

Beran Nebbiolo Nebbiolo Lampia CVT142 100,00% 18 18 0 0 BLACK yes fromBera

FRA139-2458Mtp1

Le Bonaparte Monique Negrun 88,89% 18 16 0 2 BLACK yes ? Mosard

FRA139-51Mtp10

Bourboulenc (thermo.)

Bourboulenc Pansea? Sconosciuta 100,00% 18 18 0 0 GREEN yes

MARXX01-Sodea-69

ferrana Bourboulenc B Pansea? Sconosciuta 100,00% 18 18 0 0 yes

ITA388-1533 INGANNACANE Sanvicetro Pianta uva nera che acinella (Alto Lario)

88,89% 18 16 0 2 BLACK yes

HRV041-SA#11-04

Crljenak kastelanski

Primitivo Primitivo (Pavone 2) 94,44% 18 17 0 0 BLACK

FRA139-146Mtp2

Pougayen Pougayen Quagliano 94,44% 18 17 0 0 BLACK yes see BoutconfiWP5

MARXX01-Sodea-11

38K Dattier de Beyrouth

Regina bianca 100,00% 18 18 0 0 no

MARXX01-Sodea-31

rosaqui Dattier de Beyrouth

Regina bianca 100,00% 18 18 0 0 yes

DEU098-1990-022

PAGADEBITO PAGADEBITO Rollo 1 94,44% 18 17 0 0 GREEN yes alreaknow

FRA139-114Mtp2

Biancone n°2 Pagadebiti Rollo 1 94,44% 18 17 0 0 GREEN yes alreaknow

FRA274-86E9 Tibouren E58 Tibouren Rossese di Dolceacqua 88,89% 18 16 2 0 BLACK yes alreaknow

DEU098-1998-107

GSCHLOFENE ROSSARA TRENTINA

Schiava Bresciana 100,00% 18 18 0 0 BLACK yes

ITA362-1710 Rotberger (Schiava grossaXRiesling) Sconosciuta nera foglia bollosa (lessona)

100,00% 18 18 0 0 BLACK true?Misn


ITA388-923 FARINELLA Raboso veronese Sconosciuta Pinasca (Raboso veronese)

100,00% 18 18 0 0 BLACK yes confiWP5

FRA139-157Mtp3

Corbeau n°2 Corbeau Sgurbà 88,89% 18 16 0 2 BLACK yes confiWP5

DEU098-1990-146

CONTE ROSSO CONTE ROSSO Soria (falsa) Montale 88,89% 18 16 0 2 RED yes

ESP080-BGVCAM1698

GARNACHO GRAN NEGRO (MORRASTEL-BOUSCHET)

Tenjuran (Tinjurin Provera)

88,89% 18 16 0 2 BLACK yes

DEU098-1993-212

SCIACCARELLO SCIACCARELLO Termarina RE 100,00% 18 18 0 0 BLACK yes confiWP5

FRA139-125Mtp1

Sciaccarello Sciaccarello Termarina RE 100,00% 18 18 0 0 BLACK yes confiWP5

ITA362-1199 Mammolo Termarina RE 94,44% 18 17 0 0 BLACK confiWP5

ITA388-881 DURAGUZZA SCIACCARELLO Termarina RE 100,00% 18 18 0 0 BLACK yes confiWP5

DEU098-1998-082

LUGLIENGA NERA Tolentina 100,00% 18 18 0 0 BLACK not checked

MARXX01-Sodea-55

ugni blanc Ugni blanc B Trebbiano toscano Grinzane D7/8

94,44% 18 17 0 0 yes

ITA388-R#1076 MARZEMINA BIANCA

MARZEMINA BIANCA

Uga du Cicu (Montale celli) (Uva 'Pianetta')

94,44% 18 17 0 0 GREEN yes

FRA139-996Mtp1

Ignea Ignea = Pirovano 185

Uva da tavola rosa 94,44% 18 17 0 0 ROSE yes

HRV041-SA#11-00

Bratkovina bijela Bratkovina bijela Uva del monaco (Maruggio)

100,00% 18 18 0 0 GREEN

AUT024-538 Frueher Waelcher Uva del re 88,89% 18 16 0 0 confiWP5

DEU098-1995-063

FRUEHER WAELCHER

KHARISTVALA KOLKHURI

Uva del re 100,00% 18 18 0 0 BLACK yes confiWP5

ITA362-978 Invernenga Uva rugra 1 88,89% 18 16 0 2 GREEN confiWP5

AGRI GE

1 2 3 4

EN RES 008.. - A

MohrenkoBundula leMohrenkoBundula b

Acronym: Grape

oenig leaf (peaf (partneoenig bunchbunch (part

egen06

ANNE

partner 1, Der 7, ITA36h (partner tner 7, ITA

EX 15 – WP

1

3

DEU098) 60) 1, DEU098

A360)

P5

1

8)

AAGRI-2006 Annexes 107

7/123

2

4

1 2

AGRI GE

Furner H Schiava g

1 Malvas2 Moscat

EN RES 008.. - A

Hottlinger (grossa (true

ia di Candiello selvati

Acronym: Grape

(misnomere name) (pa

ia faux (knco (true na

egen06

) (partner artner 7, IT

nown misnoame) (partn

1

1

1, DEU098TA360)

omer) (partner 7, ITA3

8)

tner 0, FRA360)

A

A179)

AGRI-2006 Annexes 1088/123

2

2

AGRI GE

1 G2 H

1 G2 T

EN RES 008.. - A

Gibi (misnoHibou blanc

GarganegaTrebbiano

Acronym: Grape

mer) (partc (true nam

a (misnomertoscano (tr

egen06

ner 3, ESPme) (partner

r) (partnerrue name) (

P080) r 15, FRA1

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1

1

179

0) ITA360)


2

2


Annex 16 : European Vitis Database: imported accessions by collections and number of described accessions by categories for the GrapeGen06 partners

Wiews code Partner

MCPD data 2011

Characteri-sation data 2011

Characteri-sation data 2011

SSR-marker

data 2011 Virus

data 2011

Photos of shoots tips, leaves and

bunches

no of maintained accessions

no of described accessions

no of accessions and repetitions

no of genotyped accessions

no of tested

accessions no of photographed

accessions

FRA139 partner00 7.502 194 208 753 71 375

DEU098 partner01 3.622 233 312 530 50 432

AUT024 partner02 927 43 50 261 104

ESP080 partner03 3.402 189 201 187 45 238

GRC010 partner04 807 105 106 199

PRT051 partner05 721 104 172 130 399

ITA388 partner06 3.186 118 152 132 29 327

ITA388p partner06 28 17 17 27 17

ITA360 partner07 546 131 193 204 88 615

ITA360p partner07 36 30 30 30

ITA362 partner08 2.455 97 129 172 105

ITA368 partner09 376 57 72 55 30 220

ITAXX01 partner10

ITA035 partner29 179 179

ITA041 partner28 15 15

CHE019 partner11 327

HUN045 partner12 1.096 142 1 HUN005 (no collection) partner13 76

CZE041 partner14 124 124 168 82 35 89

FRA274 partner15 1.756 1.011

CYP001 partner16 92 9 9 76

SVK042 partner17 151 151 151

SVN019 69

MDA004 partner18 421

AZE015 partner19

HRV041 partner20 100 100 100 100 99 238

GEOXX01 partner21

GEO013 partner22

GEO014 partner23 147 55

MARXX01 partner24

Total 28.085 1.702 2.070 4.086 464 3.473


Annex 17 : European Vitis Database: imported accessions by collections and number of described accessions by categories for the non GrapeGen06 partners.

Wiews code Partner

MCPD data: no of maintained

accessions no of described

accessions

no of accessions and

repetitions

SSR-marker

dataVirus data

2011 Photos

UKR050 Black Sea Collections 346

RUS 02 Black Sea Collections 500

GEO015 Black Sea Collections 40 25 25 53

GEO017 Black Sea Collections 251



AZE007 Black Sea Collections 397

ARM011 Black Sea Collections 133

BGR013 Genres081 1.673

ESP047 Genres081 1.577 68 107

GRC005 Genres081 259 87 131 205

Subtotal Non Grapegen06 5.605 180 263 258

Total All 33.690 1882 2.333 4.086 464 3; 731

A

AGRI GE

Annex 18 :

EN RES 008.. - A

Listing of (1cate

Acronym: Grape

1) downloadaegories to be

egen06

ble explanatifound on the

ions on descre European V

riptor recordiVitis Database

A

ing and (2) exe homepage.

AGRI-2006 Annexes 112

xcel file form

2/123

ats for all


Annex 19: European Vitis Database: content and data retrieval

The following modules have been implemented (2007-2011):

Content and search options Access levels web site:

Public search All partner access Partner specific access

MCPDs: Download of complete FAO/IPGRI -EURISCO descriptors in

combination with Vitis specific descriptors MCPDs excel file for download Quick search Advanced search in combination with characterization and

SSR-marker data

Characterisation, SSR-marker, virus status: Download of descriptors Descriptor excel file for download Search

Photos: Search

On-farm evaluation, Vitis sylvestris populations and plants Download of descriptors (Vitis sylvestris only) Descriptor excel file for download Search

Upload of data from excel files / photos: MCPD data Descriptor data SSR-marker data Virus status data Photos

Download (csv) of search results: MCPD data Descriptor data SSR-marker data Virus status data On-farm evaluation Vitis sylvestris population and plants

On-line working: - MCPD-data sets

Addition Modification Deletion

- Characterisation, SSR-marker, virus status data sets, photos Deletion

Print view Download of cultivar specific pdf-documents

Handbook Download of a manual


Annex 20 :

WP7.3. Letter to local authorities with competence on the identified wild grapevine populations

Dear Sirs, The project GrapeGen 06 (http://www1.montpellier.inra.fr/grapegen06/accueil.php) is a European project with the aim to systematize the conservation of the Vitis vinifera genetic resources in Europe and involves 24 international research centers from 17 countries. The objectives of this project are to characterize and preserve the genetic resources of this species and to make this information available on a database, the European Vitis Database (http://www.eu-vitis.de/index.php). As Partners of the Project GrapeGen06, we know that there are populations or single plants of wild grapevine (Vitis vinifera subsp. sylvestris) in your Natural Parks or in your countryside. This subsp. is the ancestral of the cultivated grapevine of Vitis vinifera subsp. vinifera (synonym Vitis vinifera subsp. sativa), with great cultural and economic importance for your country and for the whole World. The conservation of this genetic resource is important to keep the biodiversity and to allow the study of the evolution and phylogeny between the two subsp., vinifera and sylvestris. The preservation of Vitis sylvestris is also important for breeding programs, using particularly their resistance characteristics. Wild grapevines are usually found in relictic populations, bearing a very low number of individuals, in river bank forests. The Vitis sylvestris can only survive in these habitats, where, during the winter, the water is on the surface, killing one of the most destructive pest of grapevine, the phylloxera. Sometimes it is also available in other types of forests. These environments have been disrupted in most cases by agriculture, human development, pests and diseases, provoking the reduction of the populations. Specifically, the threat over this subsp. is the threat over the riparian wood, being easy to destroy them when, during the cleaning of the waterline, the roots of the Vitis sylvestris are plucked from the soil. The conservation of their natural ecosystems would also contribute to the preservation of other vegetal and animal species. The populations of Vitis sylvestris are identified by these characteristics:

1. The plants are dioecious, with male and female plants, while the subsp. vinifera is a monoecious (hermaphrodite) plant;

2. Loose and small bunches; 3. Small black berry grapes (mainly); 4. All plants in a population are different due to the sexual multiplication; 5. The relation between width and length of the seed is more than 0,65.

These characteristics allow to distinguish the real Vitis sylvestris from Vitis riparia hybris rootstocks, escaped for cultivation: in general, they are very vigorous, large in size, abundant in number and healthy. That is why preliminary botanical-ampelographic characterization of Vitis sylvestris is essential before creating preservation procedures on it.


Moreover, many laboratories within the GrapeGen06 project have the experience and facilities to analyze the genetic material of grapevines in order to identify potential wild germplasm. They are available for supporting decisions related to the management of uncharacterized accessions. In conclusion, wild grapevine is a valuable resource that keeps the genetic variation for future survival of viticulture and we encourage you to preserve the populations or plants under your safeguard. We are willing to help in what concerns the preservation of the Vitis vinifera subsp. sylvestris. In order to facilitate the preservation of the wild grapes found in your area, we provide you with the map of the area where Vitis sylvestris was detected. Best regards,

José Eduardo Jorge Eiras-Dias Laura Gaforio Nebreda

Stella Grando Ferdinand Regner David Maghradze

Andreas Jung


ANNEX 21–List of the material exchanged during the 4 years of the project

Pays - Institut nb partner Number of

accessions send Nb of accession

received Pays type de matériel Year

Germany, Geilweilerhof IRZ P01 30 - France, INRA Cuttings 2006

Autria HBLAuBA P02 7 - France, INRA Cuttings 2006

Croatia P20, 4 - France, INRA Cuttings 2006

Spain, Madrid IMIDRA P03 24 - France, INRA Cuttings 2006

Georgia, Tbilissi D. Magrazhe 86 Italy, Milan Cuttings 2006

Italy, Udine P09 13 - France, INRA Cuttings 2006

Total Year 2006 164

Spain, Madrid IMIDRA P03 10 - France, INRA Cuttings 2007

Hungary, Gödöllo P12 2 - France, INRA Leaves 2007

Italy Udine P09 2 - France, INRA Cuttings 2007

Switzerland, ACW P17 - 2 France, INRA Cuttings 2007

Germany, Geilweilerhof IRZ P01 - 12 France, INRA Cuttings 2007

Marocco, Université tétouan P24 - 14 France, INRA Cuttings 2007

Total Year 2007 14 28

Total since beginning 206

Germany, Geilweilerhof IRZ Rudolf Eibach 22 4 France, INRA Cuttings 2008

Spain, Madrid IMIDRA Gregorio Muñoz et Felix Cabello 16 15 France, INRA Cuttings 2008

Spain, Madrid IMIDRA Gregorio Muñoz et Felix Cabello 3 7 Germany, BAZ-IRZ Cuttings 2008

Spain, Madrid IMIDRA Gregorio Muñoz et Felix Cabello 7 Portugal, ENV Cuttings 2008

Spain, Madrid IMIDRA Gregorio Muñoz et Felix Cabello 5 Italy, ISMA Cuttings 2008

France, IFV P14 - 11 France, INRA Cuttings 2008

Georgia, Tbilissi D. Magrazhe 20 Italy, Milan Cuttings 2008


Italy, CRA-CRV Conegliano Mirella Gust 1 - France, INRA Cuttings 2008

Slovakia, VSSVVM P16 53 France, INRA Cuttings 2008


Total since beginning 370

Germany, Geilweilerhof IRZ Rudolf Eibach 30 France, INRA Cuttings 2009

Autria, HBLAuBA Ferdinand Regner 8 France, INRA Cuttings 2009

Spain, Madrid IMIDRA Gregorio Muñoz 24 5 France, INRA Cuttings 2009

Spain, Madrid IMIDRA Gregorio Muñoz et Felix Cabello 4 Germany, BAZ-IRZ Cuttings 2009

Spain, Madrid IMIDRA Gregorio Muñoz et Felix Cabello 1

Italy, IASMA San Michele All'Adige Cuttings 2009

Spain, Madrid IMIDRA Gregorio Muñoz et Felix Cabello 4 Cyprus, ARI Cuttings 2009


Hungary, Gödöllo Erzsébet Kiss 27 France, INRA Cuttings 2009

Hongrie University of Pécs Pal Kozma 5 France, INRA Cuttings 2009

Italy, CNR-IVV Anna Schneider 11 3 France, INRA Cuttings 2009 Italy, IASMA San Michele All'Adige

Silvia Vezzuli et Ricardo Velasco 2 France, INRA Cuttings 2009

Italy, CRA-CRV Conegliano 1 Italy, CRA-CRV Conegliano

Italy, CRA-CRV Conegliano Mirella Gust - 2 France, INRA Cuttings 2009

Marocco, Université tétouan P24 - 15 France, INRA Cuttings 2009


Total since Beginning 519

Germany, Geilweilerhof IRZ P01 37 20 France, INRA Cuttings 2010

Autria HBLAuBA P02 2 France, INRA Cuttings 2010

Spain, Logroño ICVV - 92 France, INRA Cuttings 2010

Spain, Madrid IMIDRA P03 70 22 France, INRA Cuttings 2010

Hungary, Gödöllo P12 56 France, INRA Leaves 2010



Italy, IASMA San Michele All'Adige P08 1 0 Spain, IMIDRA Cuttings 2010 Italy, IASMA San Michele All'Adige P08 10 27 France, INRA Cuttings 2010

Italy, CNR-IVV P07 1 France, INRA Cuttings 2010

Italy, Udine P09 39 France, INRA Leaves 2010

Italy, Udine P09 12 8 France, INRA Cuttings 2010

Portugal, EVN P05 - 20 France, INRA Cuttings 2010

Switzerland ACW P17 15 6 France, INRA Cuttings 2010

Germany, Geilweilerhof IRZ P1 - 15 France, INRA Cuttings 2011

Spain, Madrid IMIDRA P2 - 3 France, INRA Cuttings 2011

Spain, CSIC - - 10 France, INRA Cuttings 2011

Italy, CRA-CRV Conegliano P06 - 1 France, INRA Cuttings 2011


Total 1029


Annex 22 : List of the wild accessions introduced in the collections

Country partner #

Nb of sylvestris introduced origin Year

France, INRA P00 25 Albères, France 2007

France, INRA P00 34 Ariège, France 2008

France, INRA P00 53 Slovakia (P16) 2008

France, INRA P00 61 Pyrennées-Atlantiques 2010

France, INRA P00 12 seeds of Pic Saint Loup 2010

France, INRA P00 1 Colmar 2010

France, INRA P00 20 Spain (P03) 2010

France, INRA P00 3 Germany 2010

France, INRA P00 1 Greece 2010

France, INRA P00 27 Italy (San Michele, P08) 2010

France, INRA P00 2 Charentes, France 2010

France, INRA P00 19 Portugal (P5) 2010

France, INRA P00 1 Gaillac, France 2010

France, INRA P00 82 Corsica , France 2010 Germany , Geilweilerhof IRZ P01 30 Ketsch, Germany 2010

Portugal, EVN P05 53 Portugal 2010

Italy, CNR-IVV P07 20 Italy 2010

Georgia, RIHVO P23 42 Georgia 2010

Total 486


Annex 23: Management plan for Vitis resources (2012-2019) During the 4 years of the project, we have performed several actions towards a better management of the grape genetic resources in Europe. More actions should be tacken towards this goal. A total of 16 actions are proposed and described below. We believe that these actions should be performed in the future to ensure safe management and increase the use of the grape genetic resources. The commission should explore ways to enable the funding of such actions.

1. Towards the definition of the exact content of the germplasm collections = the European non-redundant cultivated grape germplasm list

In 2011, the “Official European Catalogue” has been published. It is an important part of the management plan since it is the first document that lists the cultivar that can and are cultivated in each country within EU. In a certain way, it is the list of the material that is today in no risk of extinction. It should also be included in the non-redundant cultivated grape germplasm list. Furthermore, in order to complete non redundant cultivated grape germplasm list the work was performed both in

the search of synonyms/homonyms in the material analyzed present in the database: for this purpose, the work done within WP1 in order to carefully analyze and check the data for errors was very determinant. It allowed the identification of 2105 unique genotypes and many potential synonyms and homonyms

work on the questionable cultivars. Within the program 177 questionables were identified and 207 still need some analysis. The work of questionable cultivar is still underway and should be continued. It will however be done on the partner own funds since the project is now finished.

Nevertheless, the work done within the grapegen06 project is only one step, since a small part of the available accessions (3.744 out of 33.000) have been analyzed. It enabled the identification of 1.737 accessions present solely in one collection throughout EU. In this respect, the definition of the exact content of the collection will only be possible when more data can be obtained from the partners. Action 1: The first step of the plan should be the definition of the list of non-redundant cultivated grape germplasm. Towars this goal, 3 sub-actions needs to be tacken: Action 1a: Now that the list of non-redundant samples is ready, the next step proposed will then be for each partner to compare the content of his database/collection with the list of unique accessions. During the duration of the project, few collections have been compared and this work has demonstrated its usefulness. Action 1b: Finally the last step should be to compare every database from every partner to the other ones in order to compare the full extend. . Action 1c: This should be at least done within the EU countries database, since the number of genotyped accessions is growing. In the future, we have now the methodologies, the experience to do it with the non EU European wine producing countries. A first step has been reach by the acceptance of the COTS action FA1003, which regroup most of all Balkan and Caucasus


countries. Money will however need to be found in order to help these countries to accomplish the analysis of their collection.

2. Towards the definition of the European non-redundant wild grape germplasm and the definition of a European law for the protection of wild Vitis sylvestris

Similarly to what should be done for cultivated germplam, a catalogue of wild germplasm both in collection and in the wild should be realized and published. The work done within this project will be a first stone to the construction of such catalogue. We have identified 218 populations & 1864 individuals. The publication of the 2011 catalogue will be done. The data is already partially available on the web. However, the publication of the catalogue has been retarded because of some animated discussions between the partners about the real danger of publishing exact location of this material. The publication of such data needs to be regulated and a list of possible recipient of the list will be drawn carefully. Action 2: The 2011 catalogue of wild individuals should be made available to a list of agencies and associations in the countries that will be quite receptive to this argument (ecologists, national parks, conservatories, .. ). But the catalogue will not be exhaustive. Prospections were done only once in many regions and in only few selected regions of each country, and as already indicated, the experience of some of the partners in this project shows that the extensive identification of wild individuals require more time. Additional countries from the Balkan and Caucasus region will also need to contribute to this work. Action 3: Additional prospections should be performed in the countries of origin of grape, in relation with scientists from these countries. Nevertheless, the survey done within GrapeGen06 project, demonstrated once again that V. sylvestris taxon is quite engendered. Two immediate actions should be taken

Action 4: Ensure the conservation of the material that was identified in a secure way : Some actions have already be taken for the introduction of some of the material in collections but we will propose additional actions (see later)

Action 5: The European commission should also ask to the European parliament to homogenize the law in the European countries. Indeed for example, in France, V. sylvestris is protected, while is it not in Spain. This taxon should be protected all over Europe !

3. Towards the development of alternative ways of conservation

During the project, we have demonstrated the feasibility of the conservation of seeds as an alternative way of reducing the risk of losing valuable genetic resources within Europe. It can now be proposed to conserve this way all female or hermaphrodite Vitis sylvestris and pure Vitis accessions. A question still remains to be resolved, but we did not include such work in Grapegen06 project: what type of seeds should be conserved? The seeds obtained from open pollination or the seeds from controlled pollination. It is also possible to collect seeds in the wild, but the difficult conditions in which the sylvestris grow in the south of Europe may have a negative impact on their conservation. Wild populations will also have to be selected in order to choose only populations showing at least 5 individuals and at least one male (56 populations in the database). Population showing at least 10 individuals and more than one male are indeed too rare (only 18). Our knowledge is indeed that in any trip, we miss numerous individuals.


Similarly, we have demonstrated that the conservation of pollen is possible, even if the fertility of the resulting pollen may be lower. A lot of pollen from male and hermaphrodite wild accessions can also be conserved in cryotanks. Pollen harvest in wild condition will also probably be interesting, even if again some local condition may have a negative impact on its conservation. Some enquiry should however be made in order to confirm this fact and propose some directives. Action 6: European centers for cryoconservation of genetic resources should be created. At the moment, only partner 0 is equipped for such conservation, but other European partners (such as Milos Faltus from Czech Republic) are also interested and should be contacted in order to have duplicates of the cryoresources. Unfortunately, it has not been possible to demonstrate the feasibility of cryoconservation of dormant bud as a possible way of conservation. Alternatives can be proposed but it is still too early to propose it as a safe alternative. Replication of valuable and endangered cultivars in more than one collection will still be the only way for ensuring the safety of European genetic resources. Action 7: Unless new data on cryoconservation of dormant bud are obtained in the future, the EU should encourage the replication of valuable and endangered cultivars in more than one collection. Action 8: In-situ actions should be promoted for a safe conservation of the wild resources, in relation with the Ecology ministries of the different countries and surely national park and conservatories.

4. Reducing the redundancy of the germplasm collections

In a longer term, when all synonymies and homonymies will be identified, it will then be possible to reduce the redundancy of the collections and to create potential space for introducing the material that need to be replicated. Action 9: Cooperation within collections should be promoted /encouraged/ funded by the EU in order to recuce the redundancy of the collections. A European collection of unique material should thus officially be defined.

5. Replication of the endangered material

The next step will then be the duplication of the endangered material. Endangered material correspond to the accessions present in only one collection, and which present a real interest (i.e: is different from any of the other accession for many molecular markers or present a really interesting phenological traits such for example resistance, or mutation of quality traits, .. ). Again, this step will need further analysis of genetic diversity and of the identification of unique genotypes in the collections. Action 10: Molecular analysis of the collections should be encouraged /funded to facilitate the comparison of collections. We can encourage all Euroepan colleagues to use the 9 markers and the coding method to identify the accessions. Action 11: Once that these accessions will be identified, we can propose to exchange them between the collections in order to have at least 2 copies of each of this material in Europe. The exchange of western material may be easier between European collections and of eastern material between non-European collections because of the quarantine problem. Anyway, potential risk of importation of grape material from Eastern countries should be assessed. It may be partially


answered by the Cost action on Phytoplasm and virus pest but additional funding may be requested for such work. Action 12: Risk of importation of grape material from Eastern countries should be assessed, in particular for the exchange of seeds. Action 13: Additional funding should also be made available to help the European countries for durable conservation of grape GR.

6. Increasing the analysis of the genetic & phenotypic diversity in the collections Until now, much work has been devoted to the SSR and ampelographic analysis of the collections. . Once the European non redundant collection will be defined, the research on GR should focus on additional analysis. The actual search of molecular markers linked to interesting traits will soon enable the identification of gene or even SNPs linked to the useful traits. At the image of the work done for example using the Ren1 resistance gene (Riaz et al, 2010), the DXS gene (Emanuelli et al, 2010) or the MybA1 gene (Fournier-Level et al, 2009), we should extend this analysis to the European Unique Collection. Similarly, SNP markers are quite interesting for the analysis of genetic diversity as demonstrated recently by Myles et al (2010). Several sets of markers will soon be available (Ibanez et al, in preparation, Nicolas et al, in preparation) and such analysis could also be promoted at the European level. Action 14: Analysis of the European collection of unique material with SNPs markers should be promoted. Precise phenotyping analysis should also be encouraged through the continuation of the work done within WP2 or the development of high throughput methodologies reducing the cost and labor necessary for this action. Nevertheless, it is our belief that the development of a core-collection that will maximize the diversity of the European germplasms (both wild and cultivated) should also become a priority. Such collection with a reduced size, but holding a large proportion of the European diversity will be a fundamental tool for the future of Europe. It would indeed be within this core-collection that we should look for the gene necessary to ensure the durability of European viticulture. It was such idea that initiated and permitted the acceptation of the COST action FA 1003. Action 15: Define core-collection(s) for improving the analysis and use of the genetic resources

7. Increasing the use of European genetic resources

This plan so far only considered the analysis and conservation of the GR in Europe. It is also vital that we increase the use of this material directly or for the breeding of new varieties. This was the work done within WP3. We have been able to prepare wines from autochthonous cultivars and propose them for degustation. Such activities need to be encouraged but the actual partners of grapegen06 may not be the only interested parties. It is also a proposition to also contact either the producers associations or the technical organisms in each country that should be good partners for increasing such activities. . Action 16: Promote on-farm analysis of the genetic resources held in the collections, in particular in the scope of the climate changes. The cultivars from southerns regions such as Spain, Portugal, Greece, could indeed be of great relevance in the future for more northerns regions.