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CyFlow® MLCyFlow® space
Immunology | Haematology
Pathology | Microbiology
Industrial Applications
Cell Biology | Cell Sorting
Multilaser 16|9 Parameters Desktop FCM Systems
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More than 40 Years of Experience and Professional Expertise
Partec (established 1967)—pioneer in Flow Cytometry since more than 40 years—responds to these requirements with the newest generation of CyFlow® analyzers and CyFlow® sorters featuring innovative computer controlled fluidic systems, modular optical bench systems with advanced PMTs for all optical channels, most modern computer and digital electronic technologies as well as realtime data acquisition and realtime data display.
In more than 100 countries worldwidewww.partec.com/worldwide
01 COMPANY
Flow Cytometry made in Germany
New sophisticated applications and increasing require-ments for reliable results in research and routine with-in the shortest possible time. The challenge for Flow Cytometry instrumen tation, automation and software. Partec. The pioneer of Flow Cytometry.
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02 INTRODUCTION
Excellence in Flow Cytometry
Ultracompact desktop high-end instruments
_ Parallel 16 bit digital pulse processing_ High fluorescence sensitivity: < 100 MESF (FITC) | < 50 MESF (PE)_ Submicron particle detection | < 0,2 µm for scatter_ RobbyWell™ Autoloader option_ True volumetric absolute counting
_ Windows™ FloMax® software for realtime data aquisition, data display and data evaluation_ Automated software compensation online and offline
_ 16 optical parameters: FSC1, FSC2, SSC, FL1–FL13_ Up to 5 light sources_ 488 nm blue solid state laser (200 mW, 50 mW or 20 mW)_ 638 nm red diode laser (25 mW or 40 mW)_ 407 nm violet diode laser (100 mW)_ Choice of UV, green and yellow lasers_ High power UV LED for highest resolution DNA analysis_ Flexible and modular system configurations
CyFlow® ML
Dimensions (cm): L 56 x W 65 x H 30 (stand alone)
Both feature:
Multilaser 16 Parameters— 13 Colors Desktop Flow Cytometer
_ 9 optical parameters—7 colors: FSC, SSC, FL1–FL7_ Up to 3 laser light sources_ 488 nm blue solid state laser (200 mW, 50 mW or 20 mW)_ 638 nm red diode laser (25 mW or 40 mW)_ 407 nm violet diode laser (100 mW)_ 375 nm UV laser (16 mW)_ Other lasers optionally available (green, yellow, etc.)_ Cell sorter upgrade option
Dimensions (cm): L 56 x W 65 x H 30 (stand alone)
3-Laser 9 Parameters—7 Colors Desktop Flow Cytometer
CyFlow® space
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The design of the flow cuvette incorporates more than
40 years of experience in handling fluids with sub-micro-
meter and nanoliter precision. Thanks to the optical and
mechanical precision of the flow cuvette, superior results are
guaranteed for all parameters, e.g. coefficients of variation (CVs)
of about 2% on all fluorescence channels. The sample is trans-
ported with the help of a computer controlled syringe pump,
part of a virtually cross-contamination-free fluid system.
The Partec CyFlow® Sorter is the result of more than 40 years of
experience in the field of Flow Cytometry for research and routine
applications in cell and particle characterization. The sorter is
used as an add-on to the Partec CyFlow® space and serves as an
optimized module for simplified and precise cell sorting.
In order to overcome the problems of droplet sorters (bio-
hazard, contamination) Partec has developed this closed
sorting system. The sorting device can be sterilized safely.
Any environmental contamination is completely avoided
under normal operation conditions. In addition, the sorting
action takes place smoothly. Fragile cells or large particles
are sorted without any distortion and other forces acting on
the cells during deflection. Long sorting runs (for several
hundred milliliters of sample volume) are possible.
The Partec Sorter has a Flow Chamber with a piezo element
and an electronic device for piezoelectric activation. The dia-
mond piezo deflects the sorted cells into a second branch of
the flow cell (see sorting scheme opposite page above).
CyFlow® Sorter as built-in module for safe closed piezo sorting offering highest purity and sorting stability.
Partec Quartz Flow Cuvette
The unique Partec quartz flow cuvette is the heart of the CyFlow® ML/space, ensuring that particles and cells cross the excitation light with the best possible precision.
FORWARD SCATTER
SAMPLESHEATH FLUID (HYDRODYNAMIC FOCUSING)
BLUE SOLID STATE LASER
RED DIODE LASER
UV OR VIOLET DIODE LASER
HIGH NA OBJECTIVES
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During piezoelectric activation, a pressure wave is generated
and, as a consequence, a small volume of sheath fluid is
deflected together with the cell or particle of interest. The
piezo activation of the sorting process is repeated each time
there is a cell to be sorted, according to the acquisition signals.
In the sample flow each cell is analyzed and all the signals
reach the computer, where the decision is made which cell
should be sorted, according to any software gating.
Special feature of the CyFlow® Sorter is the correct sorting
of ALL the cells in a region. The high yield is a result of the
practically zero dead time data acquisition system. No event
is lost because all Partec hard- and software acquisition work
without any “interrups”. In addition, in this way no “wrong” cell
can be sorted by mistake, achieving directly highest purity.
The Partec sorting principle is utmost stable, lasting,
constant, and without vibrations, because the whole system
is closed, non-elastic, and without air. The delay time setting
can be controlled easily on the CCD camera screen, no
microsopic check is needed. The Partec sorter works with
low pressure differences in the capillary system. The mor-
phology and vitality of cells (for e.g. most fragile particles
like plant protoplasts) are not influenced by sorting.
As far as the control of the sorting process is concerned,
Partec instruments have the advantage of an extremely
precise and stable system control of the time delay between
measurement and piezo activation. Another unique feature of
Partec instruments is the built-in CCD camera, which allows
an easy check of the whole process directly in the monitor of
the instrument, this is also maintained while sorting (See the
photograph above: CCD camera shot of the sorting process.
Sorted cells are deflected to the upper right).
Sorting Control Unit
Setting of sorting parameters for piezo crystal energy, delay,
slope, and hold time. Direct connection to processor.
Sorting speed
Input up to 10,000 cells per second, for highest purity (99%)
of sorted fraction up to 100 cells per second (300 cells per
second at 95% puritiy).
Sorting flow cell specifications
Completely closed quartz type flow cell with 200 microns
sorting channel for particle sizes up to 60 microns diameter
(other sorting channel dimensions optional).
Sorting action
Piezo crystal for controlled particle deviation. Adjustable
delay between point of analysis and deflection.
CyFlow® Sorter Module
DIAMOND PIEZO FOR CLOSED CELL SORTING
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Laboratories demand highest flexibility for upcoming applications. The CyFlow® ML/space open system architec-ture is designed for today and the future.
Taking advantage of most recent computer and electronic technologies
Each optical channel is equipped with an independent processor
controlled digital pulse analyzer for parallel pulse processing in
order to minimize signal losses due to coincidence and dead-
time effects. 16 bit (65536 channels) analogue-to-digital conver-
ters for each parameter are the fundament for software-based
color crosstalk compensation algorithms and other numerical
operations, e.g. fluorescence ratio measurements, with out artif-
acts. The computing power of the CyFlow® ML/space system
allows signal analysis, processing and display of each event
generated by a particle in realtime, which is a prerequisite for
precise high speed analysis and accurate absolut counting.
All-in-one design and modular extension for future cytometric analysis
The CyFlow® ML/space in its ultracompact design incorpo-
rates all modules and components within smallest dimen-
sions of L 56 x W 65 x H 30 [cm]. In contrast to other avail-
able FCM systems, the CyFlow® line of instruments does
not require any external equipment. Power supply, pressure
regulators and the fluidic system are completely built-in.
Additional space beside or under your laboratory desk is not
any longer required. In order to be prepared for future appli-
cations, the CyFlow® ML/space offers flexible and modular
configurations in a most compact system architecture.
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The True Volumetric Absolute Counting (TVAC) is a unique feature of all Partec Flow Cytometers, offering highest absolute counting precision and accuracy.
Selection of Fluorochromes for the CyFlow® ML/space Light Source System
The CyFlow® ML/space features True Volumetric Absolute
Counting for precise cell and particle enumeration. This
unique method is based on the fundamental definition of
absolute counts respectively the particle concentration
(c = N/V). In the CyFlow® ML/space, the sample volume
is measured directly by mechanical means, rather than
by calibration with oftenly expensive beads with a “given”
nominal concentration. Thus, the volume measurement is
eliminating any error influence related to eventually varying
bead concentrations or bead aggregations. The CyFlow® ML/
space allows the analysis of a fixed volume as defined by the
distance between two platinum electrodes. Alternatively,
a well defined volume of free choice involving the digital
sample speed control can be used. Benefits of True Volumetric
Absolute Counting:
_ digital volumetric precision by mechanical design: CV < 2%
_ no errors related to calibration _ no additional time and preparation steps_ no expenses for calibration beads _ no separate haematology counter required
Excitation (nm) Detector Exemplary Dyes Exemplary Dyes Exemplary Dyes Exemplary Dyes Exemplary Dyes Exemplary Dyes Exemplary Dyes Exemplary Dyes
Blue 488 Green FITC GFP Alexa Fluor 488 Syto 9-24 Oregon Green JC-1 (monomers) DiOC6(3) H2-DCF-DA
Orange PE YFP Hydroethidine
Orange Red PE-Texas Red PI ECD EB JC-1 (aggregates)
Red I PE-Cy5 PerCP PE-Dy647 Acridine Orange 7-AAD
Red II PE-Cy5.5 PerCP-Cy5.5
Far Red PE-Cy7
Red 638 Red I APC APC-Cy5 Syto 59-63 Dy647 TO-PRO3 Alexa Fluor 647 Draq5 Cy5
Red II APC-Cy5.5 Cy5.5
Far Red APC-Cy7 APC-H7 Alexa Fluor 750 Cy7
Violet 407 Blue Pacific Blue Alexa Fluor 405 Monobromobimane Hoechst 33342
Green AmCyan CFP Qdot 525 Lucifer Yellow
Orange Cascade Yellow Pacific Orange Qdot 585
UV LED365
UV Laser355375
Blue DAPI Hoechst 33342 Alexa Fluor 350 Cascade Blue BFP AMCA Indo-1 (Ca++)
Green Qdot 525
Orange Qdot 585 Indo-1 (no Ca++)
Green 532 Orange mStrawberry DsRed DY590 mOrange
Red mCherry LDS 751
Yellow 561 Orange PE Dy590 Philippin Cy3 mBanana
Red PE-Cy5 PI LDS 751
Orange 594 Orange Red Texas Red Alexa Fluor 594
Red APC mCherry Cell Tracker Red
Far Red APC-Cy7 mPlum mRaspberry mKate Katushka HCRed HCRed
03 OVERVIEW
Fields of Applications
Overview of applications and upgrade options for CyFlow® ML and CyFlow® space
CyFlow® ML CyFlow® space
Healthcare Immunology, Hematology, Pathology, Cancer Research, DNA Analysis
MicrobiologyCell Counting & Live/Dead Analysis in Biotechnology and Cell Culture,
Toxicology, Biomonitoring, Virus Detection, Research
Industrial Applications Quality Control in Food and Beverage Industry, Yeast Analysis, Fermentation Control,
Process Optimization, Particle Counting, Particle Size Distribution
Agrosciences Ploidy Analysis, Plant Genome Size, Apomixis
Cell Counting eukaryotic cell cultures (mammalian cell cultures | plant cell cultures),
prokaryotic cell cultures, leukocyte counting in depleted blood products, counting of immunolabelled subtypes, sperm cell counting
Functional Assays cell cycle and cell proliferation, viability | Live/Dead cell counting, apoptosis
Wine Industry Rapid and Automatic Detection, Quantifi cation and Live/Dead Analysis
of Yeasts in Wine & Champagne
HIV Monitoring and AIDS Patient Follow-up Diagnostics | Dedicated,
Accurate and Affordable CD4 and CD4% Testing for Adults and Children
Cell Sorting
Options & Upgrades RobbyWell™ Autoloader RobbyWell™ Autoloader CyFlow® Sorter for closed and non-hazardous, non-destruc-tive cell and particle sorting
Notes High-End FCM system for a wide range of applications
High-End FCM system for a wide range of applications and cell sorting
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Full flexibility and automation by the multi-tube FloMax® panel system and the FloMax® Report system within the FloMax® software package.
Ready prepared and freely adaptable instrument settings
and panels facilitate switching between different applica-
tions. FloMax® is optimized for immunophenotyping, micro-
biology analysis, cell cycle, DNA ploidy, and scientific flow
cytometric analysis. Data are stored in FCS Flow Cytometry
standard file format for easy exchange with other analysis
software. One of the unique features is the digital on- and
offline color crosstalk compensation of the spectral over-
lap of fluorescence from simultaneously analyzed dyes.
The N-color compensation algorithm allows a correc-
tion of the crosstalk between any parameters without the
need of rerunning a sample. FloMax® optimally supports
the True Volumetric Absolute Counting feature of the
CyFlow® ML/space, displaying particle concentrations for
any subsets of cells, even if defined by a gate at a later time
after the acquisition.
The flexible FloMax® Panel system allows automated analy-
sis of repeating sample series employing different dyes or
instrument settings. The FloMax® Report system generates
easy-to-adapt automated single or multi-tube panel results.
Reports are based on Microsoft Word or Excel written in
Visual Basic.
The Windows™ FloMax® software integrates instrument control including acquisition, on- and offline data analysis, on- and offline compensation into a complete software package.
04 ANALYZE
CyFlow® ML/space FCM System
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05 SPECIFICATIONS
CyFlow® ML/space FCM System
GENERAL_ compact flow cytometer for automated sequential analysis of single cells and microscopic particles_ scatter particle size range: 0.2 µm–200 µm_ fluorescence sensitivity: <100 MESF (FITC) < 50 MESF (PE) _ fluorescence resolution: CV < 2% _ configurations with up to 16/9 optical parameters + time parameter
LIGHT SOURCES_ up to 5/3 light sources_ blue solid state laser: 200 mW@488 nm_ red diode laser: 40 mW@635 nm_ violet diode laser: 100 mW@407 nm_ green DPSS laser 30/100 mW@532 nm_ yellow solid state laser 100 mW@561 nm_ UV LED 365 nm or ultra-violet diode laser 16 mW@375 nm_ other laser power and laser wavelengths available
OPTICS_ modular optical system with up to 16/9 optical
parameters with selected PMTs with integra-ted electronic preamplifier for CyFlow® ML: FSC1, FSC2, SSC, FL1-FL13CyFlow® space: FSC1, SSC, FL1-FL7
_ standard setup and filters_ color CCD camera for video flow monitor_ standard objective mount with high numerical aperture_ immersion gel coupling, e.g. for detection of weak cytokines (option) _ separated intermediate image planes for optimized spatial filtering by diaphragms
FLOW SYSTEM_ synthetic quartz flow cuvette for laminar sample transport with sheath fluid
_ sample port with computer controlled BioSafety cleaning system, avoids sample droplets and minimizes cross contamination_ True Volumetric Absolute Counting based on mechanical volume measurement, no need for reference particles _ contamination-free computer controlled precision syringe pump for sample transport and True Volumetric Absolute Counting, pump speed continuously adjustable from 0-50 µl/s, sheath fluid pressure continuously adjustable from 0-800 mbar_ easily accessible sheath fluid and waste
reservoirs with fluid level sensors
ELECTRONICS _ parallel signal processing for each of the optical channels with selectable linear, 3- or 4-decade logarithmic scale_ pulse height, area, and width analysis for doublet discrimination _ 16 bit analog-to-digital converters, trigger on any parameter or parameter combinations
COMPUTER _ latest industry standard computer _ 19" TFT LCD display_ CCD video camera _ dual screen setup (optional)_ DVD-RW_ keyboard, mouse, barcode reader (optional)_ 100 MB/s and 1000 MB/s Ethernet connection_ DeskJet color printer, b&w or color laser printer (optional), printing via network
SOFTWARE_ Microsoft Windows™ operating system with full network support _ Apple® Macintosh network connection (optional)_ 32 bit Windows™ FloMax® software for routine and research applications_ 64 parameter realtime data acquisition
and analysis
_ 3-spot acquisition from spatially separated light sources with time-window delay system
_ internal floating point numerics with double precision
_ one and two parameter histograms and dotplots_ 64—32768 channels resolution for 1P histograms_ 32/32—1024/1024 channels for 2P dotplots_ multiparameter N-colors crosstalk compen-
sation, settings may be corrected at any time_ multiparameter ColorGating_ ratio measurement_ doublet discrimination based on pulse height, area, and width analysis_ peak and cluster analysis and statistics_ connection to 96 well plate automate_ DNA cell cycle analysis_ DNA peak analysis_ multitube panel system with automated
acquisition_ Flow Cytometry standard data format (FCS) for storage of original and evaluated data _ storage of gates and screen layout _ automated data transfer of graphics, statistics and instrument settings to Word, Excel, PowerPoint and other desktop publishing systems or laboratory information systems (LIS)
Length 560 mm
Width 650 mm
Length 560 mm
Hei
ght 3
00 m
m
Wid
th 6
50 m
mH
eigh
t 300
mm
For in vitro diagnostic use.
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High Quality—Made in Germany
Quality, perfor mance, precision
and affordability of Partec pro-
ducts profit from a unique produc-
tion depth realized at Partec for
manufacturing modules and com-
ponents in optics, electronics, laser
technology, fluidics, and mechanics.
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4.10
Microbiology/ Industrial Applications
Agrosciences/Animal Research/Aquaculture
Medical Diagnostics Diagnostics for HIV/AIDS, TB, Malaria
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Phone +49 (0) 2534 8008-0 Fax +49 (0) 2534 8008-90 [email protected]
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