7/28/2019 Paper Virology
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Paper (Practical) MICR 502 3(2-2)GENERAL AND SYSTEMIC
VIROLOGY
Q 1: - Why we use chromium Chloride in IHA? (2)Q 2:- Define 4HA;
calculate 8HA of a sample if the titer of sample is 1: 1024. (4)
Ans (128)Q 3:- Define secondary cell line (2)
Q 4:- Draw a figure representing different routes of
inoculation. (4)Q 5:- You are provided a solution of 100mM of
tannic acid, calculate the conc. of tannic acidRequired for PHA in
20ml final volume. (2) (.01 or .001)
Q 6:- Draw figures for Indirect, direct, Sandwich Indirect and
sandwich Direct ELISA and alsolabel them. (4)
Q 7:- Why we use monoclonal Abs in sandwich ELISA. Define
Conjugate? (2)Q 8: - Enlist name of four techniques to concentrate
virus. (2)Q 9: - Enlist names of four cell lines (Please write
complete names not abbreviations)(2)Q 10:- Deign an experiment in
order to know protective Ab titer against a virus. (6)
Paper (Practical) MICR 502 3(2-2)
GENERAL AND SYSTEMIC VIROLOGYQ 1: - Why we use chromium Chloride
in IHA? (2)Q 2:- Define 4HA; calculate 8HA of a sample if the titer
of sample is 1: 1024. (4)Q 3:- Define secondary cell line (2)Q 4:-
Draw a figure representing different routes of inoculation. (4)Q
5:- You are provided a solution of 100mM of tannic acid, calculate
the conc. of tannic acid
Required for PHA in 20ml final volume. (2)Q 6:- Draw figures for
Indirect, direct, Sandwich Indirect and sandwich Direct ELISA and
also
label them. (4)Q 7:- Why we use monoclonal Abs in sandwich
ELISA. Define Conjugate? (2)Q 8: - Enlist name of four techniques
to concentrate virus. (2)
Q 9: - Enlist names of four cell lines (Please write complete
names not abbreviations)(2)Q 10:- Deign an experiment in order to
know protective Ab titer against a virus. (6)
Paper (Practical) MICR 502 3(2-2)GENERAL AND SYSTEMIC
VIROLOGY
Q 1: - Why we use chromium Chloride in IHA? (2)Q 2:- Define 4HA;
calculate 8HA of a sample if the titer of sample is 1: 1024. (4)Q
3:- Define secondary cell line (2)Q 4:- Draw a figure representing
different routes of inoculation. (4)Q 5:- You are provided a
solution of 100mM of tannic acid, calculate the conc. of tannic
acid
Required for PHA in 20ml final volume. (2)
Q 6:- Draw figures for Indirect, direct, Sandwich Indirect and
sandwich Direct ELISA and alsolabel them. (4)Q 7:- Why we use
monoclonal Abs in sandwich ELISA. Define Conjugate? (2)Q 8: -
Enlist name of four techniques to concentrate virus. (2)Q 9: -
Enlist names of four cell lines (Please write complete names not
abbreviations)(2)Q 10:- Deign an experiment in order to know
protective Ab titer against a virus. (6)
