Paper Reference Tissue Type Marker Stained Product ......Donaldson, Elena Cáceres, Anna E Hunter, Christopher Cowing-Zitron, Lynley D Pound, Michael W Adams, Andreas Zembrzycki, Kevin

Paper Reference Tissue Type Marker Stained Product # Company Dilution

Pancreas (OCT) actin Millipore 1:1000

Pancreas (OCT) active/dephosph

orylated b-

catenin

Millipore 1:500

Pancreas (OCT) total b-catenin Millipore 1:1000-

2000

Pancreas (OCT) BrdU Accurate

Chemicals

1:200

Pancreas (OCT) caspase 3 active Abcam 1:500

Pancreas (OCT) CK-19 Abcam 1:100

Pancreas (OCT) cyclin D1 Cell Signaling 1:500

Pancreas (OCT) p9GSK3b Cell Signaling 1:500

Pancreas (OCT) total GSK3b Cell Signaling 1:500

Pancreas (OCT) Disc1 Novus Biologics 1:150-250

Pancreas (OCT) Sox9 Novus Biologics 1:200

Pancreas (OCT) Glucagon Sigma-Aldrich 1:200

Pancreas (OCT) Insulin Dako 1:250

Pancreas (OCT) Pdx1 R&D Systems 1:200

Pancreas

(Parrafin)

Cyclin-D1 unknown Abcam Unknown

Pancreas

(Parrafin)


Pancreas

(Parrafin)


Taniguchi K, Russell MA, Richardson SJ,

Morgan NG. (2015) The subcellular

distribution of cyclin-D1 and cyclin-D3

within human islet cells varies according

to the status of the pancreas donor.

Diabetologia. 2015 Sep;58(9):2056-63.

doi: 10.1007/s00125-015-3645-1. Epub

2015 Jun 9

Jurczyk A, Nowosielska A, Przewozniak

N, Aryee KE, DiIorio P, Blodgett D, Yang

C, Campbell-Thompson M, Atkinson M,

Shultz L, Rittenhouse A,Harlan D,

Greiner D, Bortell R. (2015) Beyond the

brain: disrupted in schizophrenia 1

regulates pancreatic β-cell function via

glycogen synthase kinase-3β. The

Journal of the Federation of American

Societies for Experimental Biology. 2015

Nov 6. pii: fj.15-279810.












Nov 6. pii: fj.15-279810.

Robert Z. Harmsa, Danielle N. Yardea,

Zachary Guinna, Kristina M. Lorenzo-

Arteagaa, Kevin P. Corleyb, Monina S.

Cabrerab, Nora E. Sarvetnick. (2015)

Increased expression of IL-18 in the

serum and islets of type 1 diabetics.

Molecular Immunology. 2015 April. doi:

10.1016/j.molimm.2014.12.012.

Pancreas IL-18 Unknown Santa Cruz

Biotechnology

Unknown

Pancreas Ucn3 Unknown In-house 1:2000

Glucagon 4031-01F EMD Millipore 1:7000

Insulin A0564 Dako

Cytomation Inc.

1:500

Pancreas

Transplant Biopsy

CD3 M7254 Dako 1:50

Pancreas

Transplant Biopsy

CD4 PA0427 Leica 1:50

Pancreas

Transplant Biopsy

CD8 M7103 Dako 1:50

Pancreas

Transplant Biopsy

CD20 M0755 Dako 1:200

Pancreas

Transplant Biopsy

Glucagon A0565 Dako 1:75

Pancreas

Transplant Biopsy

Insulin A0564 Dako 1:50

Pancreas

Transplant Biopsy

IP-10 ab9807 Abcam 3ug/ml

Pancreas

Transplant Biopsy

CD68 M0814 Dako 1:100

Pancreas

Transplant Biopsy

IFN-alpha 21100-1 PBL 1:50

Vendrame F, Pileggi A, Laughlin E,

Allende G, Martin-Pagola A, Molano

RD, Diamantopoulos S, Standifer N,

Geubtner K, Falk BA, Ichii H,

Takahashi H, Snowhite I, Chen Z,

Mendez A, Chen L, Sageshima J, Ruiz

P, Ciancio G, Ricordi C, Reijonen H,

Nepom GT, Burke GW 3rd, Pugliese A.

(2010) Recurrence of type 1 diabetes

after simultaneous pancreas-kidney

transplantation, despite

immunosuppression, is associated with

autoantibodies and pathogenic

autoreactive CD4 T-

cells. Diabetes. 2010 Apr;59(4):947-

57. doi: 10.2337/db09-0498. Epub

2010 Jan 19.

Talitha van der Meulen, Cynthia J

Donaldson, Elena Cáceres, Anna E

Hunter, Christopher Cowing-Zitron,

Lynley D Pound, Michael W Adams,

Andreas Zembrzycki, Kevin L Grove,

Mark O Huising. (2015) Urocortin3

mediates somatostatin-dependent

negative feedback control of insulin

secretion Nature Medicine 21, 769-776












Nov 6. pii: fj.15-279810.

Pancreas

Transplant Biopsy

MCP-1 ab9858 Abcam 10ug/ml

Pancreas Insulin Unknown Abcam Unknown

Pancreas Glucagon Unknown Abcam Unknown

Pancreas Survivin Unknown Abcam Unknown

Pancreas Insulin 4011-01 Linco 1:200

Pancreas Glucagon Gift M. Appel 1:2000

Pancreas Ki67 558615 BD biosciences 1:200

Pancreas CD3 A0452 Dako 1:50

Pancreas mCD4 Unknown Unknown Unknown

Pancreas hCD4 Unknown Unknown Unknown

Pancreas mCD45R/B220 Unknown Unknown Unknown

Pancreas hCD19 Unknown Unknown Unknown

Pancreas mCD31 Unknown Unknown Unknown

Pancreas mMAdCAM Unknown Unknown Unknown

Pancreas Mlyve-1 Unknown Unknown Unknown

Pancreas Murine

Fibroblast

Unknown Unknown Unknown

Pancreas PNAd Unknown Unknown Unknown

Pancreas Insulin A056401 Dako 1:100

Pancreas Glucagon SAB4501137 Sigma 1:100

Pancreas Chemokines Unknown Unknown 10-15

ug/mL

Vendrame F, Pileggi A, Laughlin E,

Allende G, Martin-Pagola A, Molano

RD, Diamantopoulos S, Standifer N,

Geubtner K, Falk BA, Ichii H,

Takahashi H, Snowhite I, Chen Z,

Mendez A, Chen L, Sageshima J, Ruiz

P, Ciancio G, Ricordi C, Reijonen H,

Nepom GT, Burke GW 3rd, Pugliese A.

(2010) Recurrence of type 1 diabetes

after simultaneous pancreas-kidney

transplantation, despite

immunosuppression, is associated with

autoantibodies and pathogenic

autoreactive CD4 T-

cells. Diabetes. 2010 Apr;59(4):947-

57. doi: 10.2337/db09-0498. Epub

2010 Jan 19.

Gianani, R., Campbell-Thompson, M.,

Sarkar, S. A., Wasserfall, C., Pugliese,

A., Solis, J. M., Kent, S. C., Hering, B.,

West, E., Steck, A., Bonner-Weir, S.,

Atkinson, M., Coppieters, K., von

Herrath, M., & Eisenbarth, G. S. (2010).

Dimorphic histopathology of long

standing childhood-onset

diabetes.Diabetologia, 53(4), 690-8.

Keenan, H.A., Sun, J.K., Levine, J.,

Doria, A., Aiello, L.P., Eisenbarth, G.,

Bonner-Weir, S., & King, G.L. (2010).

Residual insulin production and

pancreatic ß-cell turnover after 50 years

of diabetes: Joslin Medalist

Study. Diabetes, 59(11), 2846-53.

Penaranda, C., Tang, Q., Ruddle, N.H., &

Bluestone, J. A. (2010). Prevention of

diabetes by FTY720-mediated

stabilization of peri-islet tertiary

lymphoid

organs. Diabetes, 59(60), 1461-8.

Sarka, S. A., Lee, C. E., Victorino, F.,

Nguyen, T. T., Walters, J. A., Burrack,

A., Eberlein, J., Hildemann, S. K., &

Homann, D. (2011). Expression and

regulation of chemokines in murine and

human type 1 diabetes.Diabetes, Dec.

30. PMID: 22210319.












Nov 6. pii: fj.15-279810.

Pancreas GLUT1 Unknown Millipore Unknown

Pancreas GLUT2 Unknown Millipore Unknown

Pancreas GLUT3 Unknown Sigma Aldrich Unknown

Pancreas Insulin A0564 Dako Unknown

Pancreas CD68 Unknown Dako Unknown

Pancreas Insulin AB63820 Abcam 1:200


Pancreas Pancreatic

Polypeptide

NB-100-1793 Novus

Biologicals

1:100

Pancreas Pancreatic

Polypeptide

A0619 Dako 1:2000

Pancreas VMAT2 AB1767 Millipore 1:100

Pancreas VMAT2 70808 Abcam 1:100

Pancreas Pan-Laminin 455 Unknown Unknown

Pancreas Laminin α1 317 Unknown Unknown


Pancreas Laminin α2 4H8 Unknown Unknown

Pancreas Laminin 332

(α3β3γ2)

Unknown ImmunDiagnosti

k

Unknown


Pancreas Laminin α4 3D12 Sorokin, L.,

unpublished

Unknown


Pancreas Laminin α5 4C7 Unknown Unknown

Pancreas 3A4 Unknown Unknown

Pancreas Laminin β1 3.00E+05 Unknown Unknown

Pancreas 3.00E+10 Unknown Unknown

Pancreas Laminin β2 489 Unknown Unknown

Pancreas Laminin β2 409 Unknown Unknown

Pancreas Collagen IV Unknown Unknown Unknown

Pancreas Perlecan A716 C11L1 Unknown Unknown

Pancreas Agrin Unknown Unknown Unknown

Pancreas Nidogen-1 Unknown Unknown Unknown

Coppieters, K. T., Wiber, A., Amirian, N.,

Kay, T. W., & von Herrath, M. G. (2011).

Persistent glucose transporter

expression on pancreatic beta cells from

longstanding type 1 diabetic

individuals.Diabetes/Metabolism

Research and Reviews, 27, 746-754.

Freeby M, Ichise M, Harris PE. (2012).

Vesicular monoamine transporter, type

2 (vmat2) expression as it compares

to insulin and pancreatic polypeptide

in the head, body and tail of the

human pancreas. Islets., 4(6):393-

397.

Korpos E, Kadri N, Kappelhoff R,

Wegner J, Overall CM, Weber E,

Holmberg D, Cardell S, Sorokin L.

(2012) The Peri-islet Basement

Membrane, a Barrier to Infiltrating

Leukocytes in Type 1 Diabetes in

Mouse and Human. Diabetes, Nov 8.

Epub ahead of print.












Nov 6. pii: fj.15-279810.

Pancreas Nidogen-2 Unknown Unknown Unknown

Pancreas Collagen I Unknown Chemicon Unknown

Pancreas Collagen III Unknown Southern

Biotech

Unknown

Pancreas Collagen VI Unknown Unknown Unknown

Pancreas Fibronectin Unknown Unknown Unknown

Pancreas Tenascin-C MT4 Unknown Unknown

Pancreas Matrillin-2 Unknown Unknown Unknown

Pancreas Fibrillin-1 Unknown Unknown Unknown

Pancreas Fibrillin-2 Unknown Unknown Unknown

Pancreas β-Dystroglycan Unknown Unknown Unknown

Pancreas Lutheran Blood

Group Protein

Unknown Unknown Unknown

Pancreas CD45 30G12 Unknown Unknown

Pancreas CD45 LB13 Unknown Unknown

Pancreas F4/80 CI:A3-1 Serotec Unknown

Pancreas CD11c N/418 Nordic Biosite Unknown

Pancreas Meca 32 antigen Meca32 Unknown Unknown

Pancreas CD31 Rb10 http://www.hcd

m.org/MoleculeI

nformatio

n/tabid/54/Defa

ult.aspx

Unknown

Pancreas Cathepsin C Unknown R&D Unknown

Pancreas Cathepsin S Unknown From Eckkehard

Weber

Unknown

Pancreas Cathepsin W Unknown Santa Cruz

Biotechnology

Unknown

Pancreas Insulin Unknown Dako Unknown

Pancreas Glucagon Unknown Dako Unknown

PLNs eIF4G 2469 Cell Signaling 1:1000

PLNs Caspase 3 9662 Cell Signaling 1:1000

Korpos E, Kadri N, Kappelhoff R,

Wegner J, Overall CM, Weber E,

Holmberg D, Cardell S, Sorokin L.

(2012) The Peri-islet Basement

Membrane, a Barrier to Infiltrating

Leukocytes in Type 1 Diabetes in

Mouse and Human. Diabetes, Nov 8.

Epub ahead of print.

Yip, L., Creusot, R.J., Pager, C.T.,

Sarnow,P., Fathman, C.G. (2012).

Reduced DEAF1 function during type 1

diabetes inhibits translation in lymph

node stromal cells by suppressing

Eif4g3. J Mol Cell Biol, Epub ahead of

print.












Nov 6. pii: fj.15-279810.

PLNs Beta-Actin conj.

HRP

13E5 Cell Signaling 1:1000

PLNs GAPDH Ab4985 Abcam 1:2500

Pancreas Insulin Unknown Dako 1:600

Pancreas Enteroviral vp1 5D8/1 Dako 1:2000

Pancreas PKR Unknown Abcam 1:1000

Pancreas Mcl1 Unknown Abcam 1:600

Pancreas Insulin 40011-01F Linco 1:1000

Pancreas Glucagon 4031-01 Linco 1:2000

Pancreas Somatostatin 13-2366 ARP 1:500

Pancreas MafA BL1225 Bethyl 1:5000

Pancreas MafB BL1228 Bethyl 1:4000

Pancreas Pdx1 gift from C. Wright 1:10000

Pancreas a-Amylase A8273 Sigma 1:2000

Pancreas Insulin Unknown Dako 1:140

Pancreas HLA-ABC R70000-01 Dako 1:100

Pancreas CD8 561946 BD biosciences 1:100

Pancreas Chromogranin A Unknown Unknown 1:500

Pancreas EV-P1 Unknown Unknown 1:1000

Pancreas dsRNA J2 Unknown Invitrogen 1:1000

Pancreas TLR3 Unknown Imgenex 1:100

Yip, L., Creusot, R.J., Pager, C.T.,

Sarnow,P., Fathman, C.G. (2012).

Reduced DEAF1 function during type 1

diabetes inhibits translation in lymph

node stromal cells by suppressing

Eif4g3. J Mol Cell Biol, Epub ahead of

print.

Richardson, S. J., Leete, P., Bone, A.

J., Foulis, A. K., Morgan N. G. (2012).

Expression of the enteroviral capsid

protein VP1 in the islet cells of

patients with type 1 diabetes is

associated with induction of protein

kinase R and downregulation of Mcl-1.

Diabetologia, Epub ahead of print.

Dai, C., Brissova, M., Hang, Y.,

Thompson, C., Poffenberger, G.,

Shostak, A., Chen, Z., Stein, R., &

Powers, A. C. (2012). Islet-enriched

gene expression and glucose-induced

insulin secretion in human and mouse

islets. Diabetologia, 55(3): 707-18.

Coppieters, K. T., Dotta, F., Amirian,

N., Campbell, P. D., Kay, T. W.,

Atkinson, M. A., Roep, B. O., & von

Herrath, M. G. (2012). Demonstration

of islet-autoreactive CD8 T cells in

insulitic lesions from recent onset and

long-term type 1 diabetes patients. J

Exp Med., 209 (1):51-60.

Anagandula M, Richardson SJ, Oberste

MS, Sioofy-Khojine AB, Hyöty H,

Morgan NG, Korsgren O, Frisk G.

(2013). Infection of human islets of

langerhans with two strains of

coxsackie B virus serotype 1:

Assessment of virus replication,

degree of cell death and induction of

genes involved in the innate immunity

pathway. J Med Virol. 2013 Nov 19.

doi: 10.1002/jmv.23835. [Epub ahead

of print]












Nov 6. pii: fj.15-279810.

Pancreas EV-VP1 Unknown Dako 1:500

Pancreas Insulin Unknown Invitrogen Unknown

Pancreas Glucagon Unknown Invitrogen Unknown

Pancreas Insulin Unknown Abcam 1:50

Pancreas Glucagon Unknown Abcam 1:100

Rowe P, Wasserfall C, Croker B,

Campbell-Thompson M, Pugliese A,

Atkinson M, Schatz D. (2013)

Increased complement activation in

human type 1 diabetes pancreata.

Diabetes Care. doi: 10.2337/dc13-

0203. Epub 2013 Sep 16.

Pancreas C4d 2222-8004 AbD Serotec Unknown

Pancreatic &

nonpancreatic

lymph nodes

AIRE Unknown gift: P.Peterson Unknown

Pancreatic &

nonpancreatic

lymph nodes

HLA-DP, DQ, DR F0817 Dako Unknown

Anagandula M, Richardson SJ, Oberste

MS, Sioofy-Khojine AB, Hyöty H,

Morgan NG, Korsgren O, Frisk G.

(2013). Infection of human islets of

langerhans with two strains of

coxsackie B virus serotype 1:

Assessment of virus replication,

degree of cell death and induction of

genes involved in the innate immunity

pathway. J Med Virol. 2013 Nov 19.

doi: 10.1002/jmv.23835. [Epub ahead

of print]

Kavishwar A, Moore A. (2013)

Sphingomyelin patches on pancreatic

beta-cells are indicative of insulin

secretory capacity. J Histochem

Cytochem. 61(12):910-9. doi:

10.1369/0022155413502792. Epub

2013 Aug 6.

Gardner JM, Metzger TC, McMahon EJ,

Au-Yeung BB, Krawisz AK, Lu W, Price

JD, Johannes KP, Satpathy AT, Murphy

KM, Tarbell KV, Weiss A, Anderson

MS. (2013) Extrathymic Aire-

Expressing Cells are a Distinct Bone

Marrow-Derived Population that

Induce Functional Inactivation of

CD4+ T Cells Immunity. 19;39(3):560-

72. doi:

10.1016/j.immuni.2013.08.005. Epub

2013 Aug 29.












Nov 6. pii: fj.15-279810.

Pancreas IL-15 ab55276 Abcam Unknown

Pancreas IL-15Ralpha Unknown Unknown Unknown

Pancreas Chromogranin A Unknown Unknown 1:500

Pancreas HEVP1 Unknown Unknown 1:1000

Pancreas J2 anti-dsRNA Unknown Scicons Hungary 1:1000


Pancreas IL-12p70 Unknown Bioss 1:40

Pancreas IL-12p35 Unknown Santa Cruz

Biotechnology

1:40

Pancreas Ki67 Unknown Unknown Unknown

Pancreas insulin Unknown Unknown Unknown

Pancreas CD3 Unknown Unknown Unknown

Pancreas glucagon Unknown Unknown Unknown

Pancreas CK-19 Unknown Dako 1:50

Pancreas Glucagon Unknown Immunostar 1:1000

Pancreas Insulin Unknown Invitrogen 1:100

Taylor-Fishwick DA, Weaver JR,

Grzesik W, Chakrabarti S, Green-

Mitchell S, Imai Y, Kuhn N, Nadler JL.

(2013). Production and function of IL-

12 in islets and beta cells.

Diabetologia., 56(1):126-35.

Hodik M, Lukinius A, Korsgren O, Frisk

G. (2013) Tropism Analysis of Two

Coxsackie B5 Strains Reveals Virus

Growth in Human Primary Pancreatic

Islets but not in Exocrine Cell Clusters In

Vitro. Open Virol J. 2013 Apr 5;7:49-56.

doi: 10.2174/1874357901307010049.

Print 2013.

Butler AE, Campbell-Thompson M,

Gurlo T, Dawson DW, Atkinson M,

Butler PC. (2013). Marked Expansion

of Exocrine and Endocrine Pancreas

with Incretin Therapy in Humans with

increased Exocrine Pancreas Dysplasia

and the potential for Glucagon-

producing Neuroendocrine Tumors.

Diabetes, Mar 22.

Chen J, Feigenbaum L, Awasthi P,

Butcher DO, Anver MR, Golubeva YG,

Bamford R, Zhang X, St Claire MB,

Thomas CJ, Discepolo V, Jabri B,

Waldmann TA. (2013) Insulin-

dependent diabetes induced by

pancreatic beta cell expression of IL-

15 and IL-15Rα. Proc Natl Acad Sci U

S A.110(33):13534-9. doi:

10.1073/pnas.1312911110. Epub

2013 Jul 31.












Nov 6. pii: fj.15-279810.

Valle A, Giamporcaro GM, Scavini M,

Stabilini A, Grogan P, Bianconi E,

Sebastiani G, Masini M, Maugeri N,

Porretti L, Bonfanti R, Meschi F, De

Pellegrin M, Lesma A, Rossini S,

Piemonti L, Marchetti P, Dotta F, Bosi

E, Battaglia M. (2013). Reduction of

circulating neutrophils precedes and

accompanies type 1 diabetes.

Diabetes. Jun;62(6):2072-7. doi:

10.2337/db12-1345. Epub 2013 Jan

24.

Pancreas Myeloperoxidase Unknown Abcam Unknown

Pancreas 12-Loa Unknown Sigma 1:10


Pancreas Glucagon Unknown Sigma 1:100

Pancreas CD31 Unknown Abcam 1:100

Pancreas Mac2 Unknown Cedarlane 1:100

Pancreas PPb Unknown Abcam 1:75

Pancreas PPb Unknown Progen 1:50

Pancreas Somatostatin Unknown Sigma 1:1000

Pancreas Vimentin Unknown Abcam 1:250

Pancreas VP1 M706401-1 Dako 1:2000

Pancreas MHC class I ab70328 Abcam 1:1500

Pancreas Insulin C#A056401 Dako 1:600

Pancreas Glucagon C#ab82270 Abcam 1:2000

Pancreas Insulin Unknown USBIO 1:200

Wojciech JG, Nadler Joseph L.,

Machida Y, Jerry L. Nadler, Imai Y, and

Morris MA. (2014) Expression pattern

of 12-lipoxygenases in human islets

with type 1 diabetes and type 2

diabetes. The Journal of Clinical

Endocrinology & Metabolism. 2014 Dec

22. [Epub ahead of print]

Krogvold L, Edwin B, Buanes T, Frisk

G, Skog O, Anagandula M, Korsgren O,

Undlien D, Eike M, Richardson SJ,

Leete P, Morgan NG, Oikarinen S,

Oikarinen M, Laiho JE, Hyöty H,

Ludvigsson J, Hanssen KF, Dahl-

Jørgensen K. (2014) Detection of a

low-grade enteroviral infection in the

islets of Langerhans of living patients

newly diagnosed with type 1 diabetes.

Diabetes. 2014 Nov 24. pii:

DB_141370.

Piran R, Lee S-H, Li C-R, Charbono A,

Bradley L M, and Levine F. (2014)

Pharmacological induction of

pancreatic islet cell

transdifferentiation: relevance to type

I diabetes. Cell Death and Disease 5,

e1357; doi:10.1038/cddis.2014.311.

Published online 31 July 2014.












Nov 6. pii: fj.15-279810.

Pancreas Insulin Unknown Santa Cruz

Biotechnology

1:200


Pancreas Somatostatin Unknown Santa Cruz

Biotechnology

1:200

Pancreas PDX1 Unknown Abcam 1:2000

Pancreas NKX6.1 Unknown Beta Cell Biology

Consortium

1:1000

Pancreas Insulin Unknown DakoCytomation 1:140

Pancreas glucagon Unknown DakoCytomation 1:130

Pancreas CD8alpha 561946 BD biosciences 1:100

Pancreas CD4 MAB379 R&D 1:100

Pancreas CD11c Unknown eBioscience 1:100


Pancreas CD45 M0701 Abcam Unknown

Pancreas Glucagon A0565 Dako Unknown

Pancreas CD20 M0755 Dako Unknown


Pancreas CD8 GR404-4 Abcam Unknown

Pancreas CD3 Unknown Abcam 10 ug/ml

Pancreas CD20 Unknown Abcam 1.8 ug/ml

Pancreas CD31 Unknown Abcam 3.5 ug/ml

Pancreas CD45 Unknown Abcam 5 ug/ml

Pancreas Synaptophysin Unknown Dako 1:100

Pancreas Insulin ab6995 Abcam 1:12000

Piran R, Lee S-H, Li C-R, Charbono A,

Bradley L M, and Levine F. (2014)

Pharmacological induction of

pancreatic islet cell

transdifferentiation: relevance to type

I diabetes. Cell Death and Disease 5,

e1357; doi:10.1038/cddis.2014.311.

Published online 31 July 2014.

Rodriguez-Calvo T, Ekwall O, Amirian

N, Zapardial-Gonzalo J, and von

Herrath MG (2014) Increased Immune

Cell Infiltration of the Exocrine

Pancreas: A Possible Contribution to

the Pathogenesis of Type 1 Diabetes.

Diabetes. Published online before print

June 19, 104. Diabetes, June 19, pii:

DB_140549.

Arif S, Leete P, Nguyen V, Marks K,

Nor NM, Estorninho M, Kronenberg-

Versteeg D, Bingley PJ, Todd JA, Guy

C, Dunger DB, Powrie J, Willcox A,

Foulis AK, Richardson SJ, de Rinaldis

E, Morgan NG, Lorenc A, Peakman M.

(2014) Blood and islet phenotypes

indicate immunological heterogeneity

in type 1 diabetes. Diabetes. Jun 17.

pii: DB_140365. [Epub ahead of

print].

Marika Bogdani, Pamela Y. Johnson,

Susan Potter-Perigo, Nadine Nagy,

Anthony J. Day, Paul L. Bollyky, and

Thomas N. Wight (2014) Hyaluronan

and Hyaluronan Binding Proteins

Accumulate in both Human Type 1

Diabetic Islets and Lymphoid Tissues

and Associate with Inflammatory Cells

in Insulitis. Diabetes Published online

before print March 27, 2014, doi:

10.2337/db13-1658 Diabetes, March

27












Nov 6. pii: fj.15-279810.

Pancreas Glucagon Unknown Unknown 1:1000

Pancreas Somatostatin Unknown Unknown 1:1000

Pancreas Pancreatic

Polypeptide

Unknown Abcam 1:1000

Pancreas TSG-6 Unknown Unknown 1:1000

Pancreas IalphaI Unknown Unknown 1:1000


Pancreas Glucagon A0565 Dako Unknown

Pancreas Somatostatin SC-13099 Santa Cruz

Biotechnology

Unknown

Pancreas GATA4 SC-1237 Santa Cruz

Biotechnology

Unknown

Pancreas Cytokeratin-19 Ab9221 Abcam Unknown

Pancreas Ki67 Ab833 Abcam Unknown

Pancreas CD68 m0876 Dako Unknown


Pancreas Nestin MAB5326 Millipore Unknown

Pancreas CD34 73403 Invitrogen Unknown

Pancreas Insulin Unknown Linco 1:200

Pancreas ATF6alpha Unknown Santa Cruz

Biotechnology

1:20

Pancreas sXBP1 Unknown In house 1:20

Pancreas P-eIF2alpha Unknown Invitrogen 1:20

Pancreas Insulin Unknown Accurate Lab 1:100


Pancreas Somatostatin Unknown Cell Marque 1:200

Pancreas B7-H4 12-5949-41 eBiosciences 1 ug/ml

Pancreas B7-H4 Unknown R&D 1 ug/ml


Pancreas Enteroviral VP1 M7064 Dako 1:2000

Pancreas ATP5B HPA001520 Sigma 1:500

Marika Bogdani, Pamela Y. Johnson,

Susan Potter-Perigo, Nadine Nagy,

Anthony J. Day, Paul L. Bollyky, and

Thomas N. Wight (2014) Hyaluronan

and Hyaluronan Binding Proteins

Accumulate in both Human Type 1

Diabetic Islets and Lymphoid Tissues

and Associate with Inflammatory Cells

in Insulitis. Diabetes Published online

before print March 27, 2014, doi:

10.2337/db13-1658 Diabetes, March

27

Ye J, Vives-Pi M, Gillespie KM (2014)

Maternal microchimerism: increased in

the insulin positive compartment of

type 1 diabetes pancreas but not in

infiltrating immune cells or replicating

islet cells. PLoS One. Jan

31;9(1):e86985. doi:

10.1371/journal.pone.0086985.

eCollection 2014.

Engin F, Nguyen T, Yermalovich A,

Hotamisligil GS. (2014) Aberrant islet

unfolded protein response in type 2

diabetes. Sci Rep. doi:

10.1038/srep04054.

Cheung SS, Ou D, Metzger DL,

Meloche M, Ao Z, Ng SS, Owen D,

Warnock GL. (2014) B7-H4 Expression

in Normal and Diseased Human Islet β

Cells. Pancreas 43(1):128-34. doi:

10.1097/MPA.0b013e31829695d2.

Richardson, S.J., Leete, P., Dhayal, S.,

Russell, MA., Oikarinen, M., Laiho, J.,

Svedin, S., Lind, K., Rosenling, T.,

Chapman, N., Bone, A.J., nPOD-V

Consortium, Foulis, A.K., Frisk, G.,

Flodström-Tullberg, M., Hober, D.,

Hyöty, H., Morgan, N.G. (2014).

Evaluation of the fidelity of

immunolabelling obtained with clone

5D8/1, a monoclonal antibody directed

against the enteroviral capsid protein,

VP1, in human pancreas. Diabetologia

57(2): 392-401.












Nov 6. pii: fj.15-279810.

Pancreas CKB HPA001254 Sigma 1:500


Pancreas Glucagon A0565 Dako 1:200

Pancreas

(parraffin)

Amylase SC-46657 Santa Cruz Unknown

Pancreas

(parraffin)

Amylin MCA11267 Serotec Unknown

Pancreas

(parraffin)

Carbonic

anhydrase 19.9

ab15146 Abcam Unknown

Pancreas

(parraffin)

Caspase-3,

cleaved

9961 Cell Signaling Unknown

Pancreas

(parraffin)

CD20 M0755 DAKO Unknown

Pancreas

(parraffin)

CD3 A0452 DAKO Unknown

Pancreas

(parraffin)

CD34 3569 Cell Signaling Unknown

Pancreas

(parraffin)


Pancreas

(parraffin)


Campbell-Thompson, M. L., Heiple, T.,

Montgomery, E., Zhang, L., Schneider,

L. Staining Protocols for Human

Pancreatic Islets. J. Vis. Exp. (63),

e4068, doi:10.3791/4068 (2012).

Reddy S, Zeng N, Al-Diery H, Jung D,

Yeu C, Joret MO, Merrilees MJ, Wu F.

(2015) Analysis of peri-islet CD45-

positive leucocytic infiltrates in long-

standing type 1 diabetic patients.

Diabetologia. 2015 Feb 17. [Epub

ahead of print]

Richardson, S.J., Leete, P., Dhayal, S.,

Russell, MA., Oikarinen, M., Laiho, J.,

Svedin, S., Lind, K., Rosenling, T.,

Chapman, N., Bone, A.J., nPOD-V

Consortium, Foulis, A.K., Frisk, G.,

Flodström-Tullberg, M., Hober, D.,

Hyöty, H., Morgan, N.G. (2014).

Evaluation of the fidelity of

immunolabelling obtained with clone

5D8/1, a monoclonal antibody directed

against the enteroviral capsid protein,

VP1, in human pancreas. Diabetologia

57(2): 392-401.












Nov 6. pii: fj.15-279810.

Pancreas

(parraffin)


Pancreas

(parraffin)


Pancreas

(parraffin)

Chromogranin A A0430 DAKO Unknown

Pancreas

(parraffin)

CK17 M7046 DAKO Unknown

Pancreas

(parraffin)


Pancreas

(parraffin)


Pancreas

(parraffin)

C-peptide 4593 Cell Signaling Unknown

Pancreas

(parraffin)

Foxp3 14-4776-80 e Bioscience Unknown

Pancreas

(parraffin)

Ghrelin SC-10386 Santa Cruz Unknown

Pancreas

(parraffin)

Ghrelin ab85104 Abcam Unknown

Pancreas

(parraffin)

Glucagon A0565 DAKO Unknown

Pancreas

(parraffin)

Glucagon ab10988 Abcam Unknown

Pancreas

(parraffin)

Glucagon T-5037 Bachem Unknown

Pancreas

(parraffin)

Glut-1 ab40084 Abcam Unknown

Pancreas

(parraffin)

Glut-2 SC-9117 Santa Cruz Unknown

Pancreas

(parraffin)

Insulin A0564 DAKO Unknown

Pancreas

(parraffin)

Ki-67 M7240 DAKO Unknown

Pancreas

(parraffin)

Mafa NB400-137A Novus Biological Unknown





e4068, doi:10.3791/4068 (2012).












Nov 6. pii: fj.15-279810.

Pancreas

(parraffin)

Pancreatic

polypeptide

18-0043 Invitrogen Unknown

Pancreas

(parraffin)

Pdx-1 ab47308 Abcam Unknown

Pancreas

(parraffin)

Proinsulin NCL-proin-

1G4

Novocastra Unknown

Pancreas

(parraffin)

Proinsulin GS-9A8 Developmental

Studies

Hybridoma Bank

Unknown

Pancreas

(parraffin)

Secretagogin HPA 006641 Sigma Unknown

Pancreas

(parraffin)

Smooth muscle

actin

ab5694 Abcam Unknown

Pancreas

(parraffin)

Somatostatin A0566 DAKO Unknown

Pancreas

(parraffin)

Synaptophysin M0776 DAKO Unknown

Pancreas (OCT) CD11b ab6332 Abcam Unknown

Pancreas (OCT) CD11c AHP1226 Serotec Unknown

Pancreas (OCT) CD25 NB 600-564 Novus Biological Unknown

Pancreas (OCT) CD94 ab61874 Abcam Unknown

Pancreas (OCT) GAD65 SC-130569 Santa Cruz Unknown

Pancreas (OCT) HLA-ABC M0736 DAKO Unknown





e4068, doi:10.3791/4068 (2012).












Nov 6. pii: fj.15-279810.

Clone #

Unknown

Unknown

Unknown

Clone #

Unknown

7218

F7.2.38

4B12

C8/144B

L26

Polyclonal

Polyclonal

Polyclonal

Kp1

MMHA-2

Clone #

ab9858

Unknown

Unknown

Unknown

Polyclonal

Unknown

B56

Polyclonal

L3T4

RPA-T4

RA3.6B2

H1B19

390

MECA367

223322

ERTR7

MECA79

Polyclonal

Polyclonal

Goat

Polyclonals

Clone #

Unknown

Unknown

Unknown

Polyclonal

Unknown

Polyclonal

Polyclonal

Polyclonal

Unknown

Polyclonal

Polyclonal

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Clone #

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Polyclonal

Polyclonal

Clone #

monoclonal

Polyclonal

Unknown

monoclonal

Unknown

Unknown

Polyclonal

Polyclonal

Polyclonal

Polyclonal

Unknown

Unknown

Polyclonal

Polyclonal

W6/32

HIT8a

Unknown

Unknown

Unknown

Unknown

Clone #

Unknown

Unknown

Unknown

Unknown

Unknown

10-11

6.1

CR3/43

Clone #

Monoclonal

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Clone #

Monoclonal

Polyclonal

Polyclonal

monoclonal

monoclonal

monoclonal

Polyclonal

Polyclonal

Polyclonal

Polyclonal

5D8/1

EMR8-5

Polyclonal

IMD-7

Unknown

Clone #

Unknown

Unknown

Unknown

Unknown

Unknown

Polyclonal

Polyclonal

HIT8a

34930

3.9

Polyclonal

Unknown

Polyclonal

L26

PG-M1

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

K36aC10

Clone #

Unknown

Unknown

Unknown

Unknown

Unknown

Polyclonal

Polyclonal

Polyclonal

Polyclonal

RCK108

Polyclonal

PG-M1

2B11 +

PD7/26

10C2

B1-3C5

Unknown

Unknown

Polyclonal

Unknown

Unknown

Unknown

Unknown

H74

Unknown

Polyclonal

5D8/1

Polyclonal

Clone #

Polyclonal

Polyclonal

Polyclonal

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Clone #

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Clone #

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Unknown

Clone #

Documents

Paper Reference Tissue Type Marker Stained Product ......Donaldson, Elena Cáceres, Anna E Hunter, Christopher Cowing-Zitron, Lynley D Pound, Michael W Adams, Andreas Zembrzycki, Kevin