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Mohsin, H. F., Abdul Wahab, I., Naz, H., Ali Shah, S. A. and Weber, J. F. F.

Faculty of Pharmacy, Universiti Teknologi MARA, 40450 Shah Alam, Selangor Darul Ehsan

Pandanaceae Research in Support for Industrial Plantation

Pandanaceae Research in Support for Industrial Plantation

2nd International Plantation Industry Conference and Exhibition (IPiCEX 2008), Shah Alam, 18th - 21st November 2008

OBJECTIVE

To present the chromatographic profile of the root extract of Pandanus sanderi.

Alkaloids, terpenoids and phytosterols are Pandanus’ major constituents.

HYPOTHESIS

2nd International Plantation Industry Conference and Exhibition (IPiCEX 2008), Shah Alam, 18th - 21st November 2008

5

2 9 8

74

1

HO3

6

10

1112

13

NO

O

CH3

O

HH

OH3C

1615

4

5

H

1820

21

2N O

O

CH3

O

HH

OH3C

1615

45H

1820

21

2

9

8

9

8 7

N

OO

CH3

O

HH

OH3C

1615

4

5

H

1820

21

29

8 7

O

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EXAMPLES OF PANDANACEAE2nd International Plantation Industry Conference and Exhibition (IPiCEX 2008), Shah Alam, 18th - 21st November 2008

•Unlike the green Pandanus amaryllifolius (pandan wangi), P. sanderi is variegated or having streaks of a different colors. •It is characterized by longitudinal yellow and green stripes of the leaves. •The yellow stripe is at the middle of two green stripes.

• In India, the leaf extract is used to cure thyroid problem and to treat fever in Taiwan (Jong, T.Jong, T.--T. et T. et al., 1998al., 1998).

• Several species were reported to act as a remedy for toothache and rheumatism (Takayama, 2000)

• Busque, (2002) reported that P. amaryllifolius was used for strengthening the heart and as a diuretic.

PHARMACEUTICAL PERSPECTIVE2nd International Plantation Industry Conference and Exhibition (IPiCEX 2008), Shah Alam, 18th - 21st November 2008

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Various compounds from Pandanaceae species.

Isolated compounds from Pandanaceae species

References

1 Polypeptide Ooi, L. S. M. et al. (2004)2 Alkaloids Salim, A. A. et al (2004)

3 Pyrrolidine Alkaloids Takayama, H. et al. (2002)

4 Alkaloids Takayama, H. et al. (2001)

5 2 phenolics, four lignans Jong, T.-T. et al (1998)

6 4-Hydroxybenzoic acid Peungvicha, P. et al (1998)7 Isopentenyl and dimethylallyl

acetates and cinnamatesI. Vahirua-Lechat et al (1996)

8 Piperidine alkaloids Nonato, M. G. et al. (1993)

9 Sesquiterpene hydrocarbons and monoterpene (linalool)

Macleod, A. J. et al. (1982)

PHYTOCHEMICAL PERSPECTIVE2nd International Plantation Industry Conference and Exhibition (IPiCEX 2008), Shah Alam, 18th - 21st November 2008

NO

O CH3

OO

CH3

N

OOCH3

OH3C

O

H

Pandamarilactone-1C18H25NO4

PandanamineC18H23NO4

NO

O

CH3

Pandamarilactonine-AC18H25NO4

O

HH

OH3C

O

OH3C

O

O

CH3

An compound which is not a "natural product" (Salim et al., 2004)

C18H23NO4

16

16

N

NH

O

HH

OH3C

norpandamarilactonine-A norpandamarilactonine-B

NH

O

HH

OH3C

PHYTOCHEMICAL PERSPECTIVE2nd International Plantation Industry Conference and Exhibition (IPiCEX 2008), Shah Alam, 18th - 21st November 2008

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• Pandanus fruits were reported to contain provitamin A carotenoids (Englberger, L. et al., 2003; 2006).

FOOD & BIOTECH PERSPECTIVE

2nd International Plantation Industry Conference and Exhibition (IPiCEX 2008), Shah Alam, 18th - 21st November 2008

METHODOLOGY

• Liquid-liquid partition

• Column Chromatography

• Thin layer chromatography

(TLC)

• Preparative TLC

• HPLC

• Spectroscopy Analysis (NMR)

2nd International Plantation Industry Conference and Exhibition (IPiCEX 2008), Shah Alam, 18th - 21st November 2008

• Sample extraction

• Fractionation of crude extract

• Isolation of pure compounds

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Outline of the normal extraction procedure

Hexane extractChloroform extractMethanol extract

2nd International Plantation Industry Conference and Exhibition (IPiCEX 2008), Shah Alam, 18th - 21st November 2008

Grinding Soaking

RESULTS & DISCUSSION

TLC of the root extract: short wave (hexane:acetone = 80:20)

TLC of the root extract: long wave (hexane:acetone = 50:50)

2nd International Plantation Industry Conference and Exhibition (IPiCEX 2008), Shah Alam, 18th - 21st November 2008

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Results and discussion : Chromatograms

(i) λ=210, 280 and 360 nm

(ii) λ=210 nm (iii) λ=280 nm (iv) λ=360 nm

C18 column: (i) 4.6 x 150 mm

C18 column: (ii) – (iv) 4.6 x 250 mm

Pandanus sanderi- Root- MeOH fraction

HPLC Agilent Technologies' Zorbax® C-18; 4.6 x 250 mm, 5 μm, CH3CN:H2O, 10 to 90%; 38 min, flow rate: 1 ml/min, λ=210, 280, 360 nm

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Pandanus sanderi- Root- Hexane fraction

HPLC Agilent Technologies' Zorbax® RX-Silica; 4.6 x 250 mm, 5 μm, Hexane:EtOAc, 10 to 90%; 40 min, flow rate: 1 ml/min, λ=210, 280, 360 nm

Results and discussion : Chromatogram

The chromatograms that were produced, with and without the utilization of the analytical guard column (4.6 x 12.5 mm).

•tR = 18 – 36 minutes could be isolated and subjected for fractionation.

•The collection methods (at λ=210 nm) are currently in progress, with the employment of the analytical guard column.

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CONCLUSION

Pandanaceae will gain more attention in pharmaceutical investigations, food research and urban landscape improvement.

Future planning for a Pandanaceae plantation could encourage research and development in industrial, environmental and eco-tourism sectors.

2nd International Plantation Industry Conference and Exhibition (IPiCEX 2008), Shah Alam, 18th - 21st November 2008

Beentje, H. 1993. Pandanaceae: Ed. R. M. Polhill. Kew Royal Botanic Gardens, Royal Botanic Gardens, Kew, R. M. Polhill, East African Herbarium, East African Herbarium, CRC Press.

Chih Min, B., Omar-Hor, K. & Chow Lin, O.-Y. 2003. 1001 Garden Plants in Singapore. National Parks, Singapore. pp 184.

Tan, M. A., Takayama, H., Aimi, N., Kitajima, M., Franzblau, S. G. & Nonato, M. G. 2008. Antitubercular triterpenes and phytosterols fromPandanus tectorius Soland. var. laevis. J Nat Med, 62, 232–235.

Mohsin, H. F., Abdul Wahab, I., Naz, H., Ali Shah, S. A. & Weber, J. F. F. Chemical Constituents Of Pandanus sanderi (Pandanaceae). National Conference on Forest Products 2008 (NCFP 2008), 28th – 31st October 2008, Kuala Lumpur.

REFERENCES2nd International Plantation Industry Conference and Exhibition (IPiCEX 2008), Shah Alam, 18th - 21st November 2008

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• Mr. Mohamad Soliheen Bin Mohd. Zaki & Mr. Mohd Rizal Bin Salleh for their technical assistance, under the university’s Student Employment Scheme.

• Ms. Suriana Hanim bt. Ariffin @ Sukri for the literature search.

• Faculty of Pharmacy UiTM, for the support.

Acknowledgements 2nd International Plantation Industry Conference and Exhibition (IPiCEX 2008), Shah Alam, 18th - 21st November 2008

Thank You…